JP3381484B2 - Sample separation device by gel electrophoresis - Google Patents
Sample separation device by gel electrophoresisInfo
- Publication number
- JP3381484B2 JP3381484B2 JP27640095A JP27640095A JP3381484B2 JP 3381484 B2 JP3381484 B2 JP 3381484B2 JP 27640095 A JP27640095 A JP 27640095A JP 27640095 A JP27640095 A JP 27640095A JP 3381484 B2 JP3381484 B2 JP 3381484B2
- Authority
- JP
- Japan
- Prior art keywords
- gel
- base
- electrophoretic
- pattern
- band
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44739—Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
Description
【０００１】[0001]
【発明の属する技術分野】本発明はライフサイエンスの
分野において、ゲル電気泳動装置により分離されたＤＮ
Ａや蛋白質などの高分子物質試料の所定の断片部分を分
取する装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention is in the field of life sciences, where DNs separated by gel electrophoresis are used.
The present invention relates to an apparatus for separating a predetermined fragment portion of a polymer substance sample such as A or protein.
【０００２】[0002]
【従来の技術】例えば遺伝子工学の分野では、標準試料
と対象試料とをゲル電気泳動させ、両試料の泳動パター
ンを比較してその相違を検証するとともに、相違してい
る部分の分子配列を決定する必要がある。そのために
は、対象試料の泳動パターンのうち、標準試料の泳動パ
ターンと相違する部分を分取する必要がある。2. Description of the Related Art In the field of genetic engineering, for example, a standard sample and a target sample are subjected to gel electrophoresis, the migration patterns of both samples are compared to verify the difference, and the molecular sequence of the different portion is determined. There is a need to. For that purpose, it is necessary to separate a portion of the migration pattern of the target sample, which is different from the migration pattern of the standard sample.
【０００３】従来は、ゲル電気泳動した対象試料のパタ
ーンのうち分取すべき断片部分のゲルを直接手動でメス
によって切り出すか、その展開した泳動パターンをいっ
たんナイロンメンブレンに転写した後、そのナイロンメ
ンブレンをはさみやメスで切り出すことによって分取し
ている。Conventionally, the gel of the fragment portion to be separated out of the pattern of the target sample subjected to gel electrophoresis is directly cut out manually with a scalpel, or the developed migration pattern is once transferred to a nylon membrane, and then the nylon membrane. Are separated by cutting out with scissors or a scalpel.
【０００４】[0004]
【発明が解決しようとする課題】従来のように泳動パタ
ーンを別の認識装置で光学的に認識し、その情報に基づ
いて所定の部分を切り出すが、泳動パターンの認識と切
り出し作業とは別の装置による独立した作業であるた
め、泳動パターン認識装置で切り出すべき部分を決めて
も、切出しの際にはその位置を再確認することが必要で
ある。またメスなどを用いて手動により行なう切出し作
業は煩雑である。そこで、本発明は泳動パターンを認識
するとともに、その同じ装置で所定部分の切出し又はそ
の部分の泳動バンドの抽出を行なえるようにすることに
より、必要な部分の切出し又は抽出を正確に、かつ容易
に行なえるようにすることを目的とするものである。As in the prior art, the electrophoretic pattern is optically recognized by another recognition device and a predetermined portion is cut out based on the information, but the recognition of the migration pattern and the cutting out work are different. Since it is an independent operation by the device, even if the migration pattern recognition device determines the portion to be cut out, it is necessary to reconfirm the position when cutting out. Further, the manual cutting work using a knife or the like is complicated. Therefore, the present invention recognizes a migration pattern and enables the same device to cut out a predetermined portion or extract a migration band of the portion, so that the necessary portion can be cut out or extracted accurately and easily. The purpose is to be able to do.
【０００５】[0005]
【課題を解決するための手段】本発明のサンプル分取装
置は、スラブ状ゲルを用いた電気泳動による試料の分離
が完了し、そのスラブ状ゲルを前後から挟んで支持して
いた２枚の支持板のうちの少なくとも一方の支持板が剥
がされた状態で露出したゲルが上向きになるように載置
される基台と、その基台の上方に配置され基台上に載置
されたゲルの所定部分を切り出す切出し器具又はその部
分の泳動バンドを電気泳動的に抽出する抽出器具と、そ
の切出し器具又は抽出器具を基台の面に平行なＸ，Ｙ方
向と基台の面に垂直なＺ方向とに移動させてその切出し
器具によるゲルの所定部分の切出し又はその部分の泳動
バンド抽出動作を行なう作業アーム機構と、基台上に載
置されたゲルの泳動パターンを光学的に検出する泳動パ
ターン検出手段と、泳動パターン検出手段の検出結果に
基づいて自動的に又は手動入力により定められた所定の
泳動バンドのゲルを切り取り、又はその泳動バンドを抽
出するように、作業アーム機構を制御する分取制御部と
を備えている。In the sample sorting apparatus of the present invention, separation of a sample by electrophoresis using a slab-like gel is completed, and the slab-like gel is supported by sandwiching it from the front and back. A base that is placed so that the exposed gel faces upward with at least one of the support plates removed, and a gel that is placed above the base and placed on the base. A cutting-out device that cuts out a predetermined part of or the extraction device that electrophoretically extracts the migration band of the part, and the cutting-out device or the extraction device that is perpendicular to the X and Y directions parallel to the surface of the base and the surface of the base. A working arm mechanism that moves in the Z direction and cuts out a predetermined part of the gel by the cutting tool or performs a migration band extraction operation of that part, and optically detects the migration pattern of the gel placed on the base. Electrophoretic pattern detection means and A fractionation control unit that controls the working arm mechanism so as to cut out a gel of a predetermined migration band determined automatically or by manual input based on the detection result of the migration pattern detection means or to extract the migration band. Is equipped with.
【０００６】[0006]
【実施例】図１は一実施例を表わしたものであり、
（Ａ）は平面図、（Ｂ）は分取制御部などをブロック図
で表わした正面図である。基台２上には既に電気泳動を
終了したゲル電気泳動板が載置されている。そのゲル電
気泳動板はポリアクリルアミド又はアガロースなどから
なるゲル４が２枚のガラス板の間に挾まれて支持されて
いたものであり、電気泳動終了後に一方のガラス板を剥
がし、残った他方のガラス板６が下になるように基台２
上に載置されている。ゲル４には試料が電気泳動により
展開して泳動バンド７によるパターンを形成している。
図１（Ａ）の例で、左側のレーンに展開しているパター
ンは標準サンプルのパターン、右側のレーンに展開して
いるパターンは対象試料のパターンとする。基台２の上
方には基台２上に載置されたゲル４の所定部分を切り出
す切出し器具８が配置され、切出し器具８を基台２の面
に平行なＸ，Ｙ方向と基台２の面に垂直なＺ方向とに移
動させて切出し器具８によるゲル４の所定部分の切出し
動作を行なう作業アーム機構１０が設けられている。作
業アーム機構１０は、切出し器具８をＺ方向、すなわち
ゲル４の肉厚方向に移動させるＺ駆動部１２と、切出し
器具８を保持したＺ駆動部１２をＸ軸方向のガイド軸１
４に沿って移動させるＸ駆動部１６と、ガイド軸１４を
Ｙ軸方向のガイド軸１８ａ，１８ｂに沿って移動させる
Ｙ駆動部２０とを備えている。EXAMPLE FIG. 1 shows an example.
(A) is a plan view, (B) is a front view showing a fractionation control unit and the like in a block diagram. On the base 2 is placed a gel electrophoresis plate that has already undergone electrophoresis. The gel electrophoresis plate was one in which a gel 4 made of polyacrylamide or agarose was sandwiched between two glass plates and supported, and one glass plate was peeled off after the electrophoresis was completed, and the other glass plate remained. Base 2 with 6 down
It is placed on top. The sample is developed on the gel 4 by electrophoresis to form a pattern by the migration band 7.
In the example of FIG. 1A, the pattern developed in the left lane is the standard sample pattern, and the pattern developed in the right lane is the target sample pattern. A cutting tool 8 for cutting a predetermined portion of the gel 4 placed on the base 2 is arranged above the base 2, and the cutting tool 8 is parallel to the surface of the base 2 in the X and Y directions and the base 2. There is provided a working arm mechanism 10 which is moved in the Z direction perpendicular to the surface of the cutting tool 8 to cut out a predetermined portion of the gel 4 by the cutting tool 8. The working arm mechanism 10 includes a Z drive unit 12 that moves the cutting instrument 8 in the Z direction, that is, a thickness direction of the gel 4, and a Z driving unit 12 that holds the cutting instrument 8 in the X axis direction of the guide shaft 1.
4 is provided with an X drive unit 16 and a Y drive unit 20 for moving the guide shaft 14 along the Y-axis direction guide shafts 18a and 18b.
【０００７】切出し器具８は図２に示されるような中空
のパイプ状のものであり、そのパイプの先端をゲル４の
肉厚方向に押しつけることによりパイプ内にゲルを詰め
込むことができる。そのパイプをゲル４の所定部分４ａ
で平面上の位置を変えながら上下方向に移動させてゲル
４の肉厚方向に押しつける操作を繰り返すことにより、
ゲル４の所定部分４ａを切出し器具８のパイプ内に順次
詰め込んでいくことができる。この例では、切出し器具
８を４回上下動させることにより、ゲル４の所定の部分
４ａから〜を切り取る。切出し器具８のパイプに詰
め込んだゲルを回収するために、パイプの上部には加圧
空気を供給する押出し機構２２が設けられており、図２
（Ｂ）のように、所定の容器２４内又はその上部に切出
し器具８のパイプを移動させ、押出し機構２２により加
圧空気を供給してパイプに詰め込んだゲルを容器２４へ
吐出する。The cutting instrument 8 is in the form of a hollow pipe as shown in FIG. 2, and the gel can be packed in the pipe by pressing the tip of the pipe in the thickness direction of the gel 4. The pipe is attached to the predetermined part 4a of the gel 4.
By changing the position on the plane and moving it up and down and pressing it in the thickness direction of the gel 4,
The predetermined portion 4a of the gel 4 can be sequentially packed in the pipe of the cutting instrument 8. In this example, the cutting tool 8 is moved up and down four times to cut through from the predetermined portion 4a of the gel 4. In order to collect the gel packed in the pipe of the cutting device 8, an extruding mechanism 22 for supplying pressurized air is provided on the upper part of the pipe.
As shown in (B), the pipe of the cutting instrument 8 is moved into or in a predetermined container 24, and pressurized air is supplied by the pushing mechanism 22 to discharge the gel packed in the pipe into the container 24.
【０００８】図１に戻って説明を続けると、ゲルに展開
した泳動パターンを光学的に認識するため、基台２の上
方には基台２上に載置されたゲル４を照射するレーザ装
置や紫外線光源などの光源３０と、ゲル４に展開した泳
動パターンを光学的に検出するための光電子増倍管や半
導体検出器などの光検出器３２が設けられている。光源
３０と光検出器３２は泳動パターン検出手段を構成して
いる。光検出器３２による検出信号をもとにして切り取
るべき泳動バンドを指定し、作業アーム機構１０を介し
てゲルの切取りを制御する分取制御部３４は、標準サン
プルの泳動パターンと対象試料の泳動パターンとを比較
し、対象試料の特異パターンの泳動バンドを指定し、そ
の指定した泳動バンドの切取り領域を切出し器具８によ
り切り取るように、Ｘ駆動部１６、Ｙ駆動部２０及びＺ
駆動部１２の駆動を制御する。表示部３６は泳動パター
ンを表示するものであり、例えば図３（Ａ）に示される
ような画像データをＣＲＴなどの表示画面に表示する。Returning to FIG. 1, for further explanation, in order to optically recognize the migration pattern developed on the gel, a laser device for irradiating the gel 4 mounted on the base 2 above the base 2 is provided. A light source 30 such as an ultraviolet light source or the like, and a photodetector 32 such as a photomultiplier tube or a semiconductor detector for optically detecting the migration pattern developed on the gel 4 are provided. The light source 30 and the photodetector 32 form a migration pattern detecting means. The preparative control unit 34, which specifies the electrophoretic band to be excised based on the detection signal from the photodetector 32, and controls the excision of the gel through the working arm mechanism 10, is used for the electrophoretic pattern of the standard sample and the electrophoretic migration of the target sample. The X drive unit 16, the Y drive unit 20, and the Z drive unit 20 compare the pattern with the pattern, specify the migration band of the specific pattern of the target sample, and cut out the cutout region of the specified migration band with the cutting instrument 8.
The drive of the drive unit 12 is controlled. The display unit 36 displays a migration pattern, and displays image data as shown in FIG. 3A, for example, on a display screen such as a CRT.
【０００９】いま、図３（Ａ）に示されるような画像デ
ータが分取制御部３４で認識され、対象試料のパターン
のうちｂ印の泳動バンドが特異パターンであり、この泳
動バンドをゲル４から切り出すものと認識したとする。
ｂ印の泳動バンドが切り取るべき特異パターンであると
いう認識は、分取制御部３４の機能を実現するコンピュ
ータのプログラムにより自動的に行なってもよく、表示
部３６に表示された画像をオペレータが見て認識し、分
取制御部３４に手動で入力するようにしてもよい。The image data as shown in FIG. 3A is now recognized by the sorting control section 34, and the electrophoretic band indicated by b in the pattern of the target sample is a unique pattern. Suppose you recognize that you want to cut out from.
The recognition that the electrophoretic band indicated by b is a peculiar pattern to be cut may be automatically performed by a program of a computer that realizes the function of the fractionation control unit 34, and the operator can see the image displayed on the display unit 36. Alternatively, it may be recognized and input manually into the sorting control unit 34.
【００１０】分取制御部３４が切取り領域を指定する場
合、その泳動バンドの平面的な濃縮度合いを光学的に解
析して、図３（Ｂ）の右側に示されるように、濃度信号
Ｉのしきい値Ｉthを設定することにより切出し領域４ａ
の大きさを同図（Ｂ）の左側の図の斜線部のように決
め、その領域内を切り出すように指示を出すようにすれ
ばよい。When the fractionation control unit 34 designates the cutout region, the planar concentration of the migration band is optically analyzed, and as shown on the right side of FIG. The cutout area 4a is set by setting the threshold value Ith.
The size of the area may be determined as shown by the shaded area on the left side of FIG. 3B, and an instruction to cut out the area may be issued.
【００１１】分取制御部３４は、自動的に認識して又は
オペレータが手動入力で指定したゲル４の所定の泳動ハ
ンド７上に切出し器具８を移動させ、Ｚ駆動部１２が切
り出し器具８を上下方向に移動させるとともに、Ｘ駆動
部１６とＹ駆動部２０によって切出し領域４ａ内を移動
させることにより、その領域４ａのゲルが切出し器具８
のパイプに詰め込まれて分取されていく。切出し器具８
に詰め込まれたゲル４ａには目的の断片試料が含まれて
いる。The sorting control section 34 moves the cutting instrument 8 onto a predetermined migration hand 7 of the gel 4 which is automatically recognized or manually specified by the operator, and the Z drive section 12 moves the cutting instrument 8 to the cutting tool 8. The gel in the region 4a is moved by the X drive unit 16 and the Y drive unit 20 while being moved in the vertical direction, and the gel in the region 4a is cut out.
The pipes are packed and sorted. Cutting device 8
The gel 4a packed in contains the target fragment sample.
【００１２】実施例のように切出し器具８としてパイプ
状のものを用いると、切出し器具をゲルの肉厚方向に上
下移動させるだけで切り出すことができ、切り出し操作
が容易になる利点がある。しかし、切出し器具はこのよ
うなパイプ状のものに限らず、メスを使って切り取るも
のであってもよい。When a pipe-shaped cutting instrument 8 is used as in the embodiment, the cutting instrument can be cut out simply by moving the cutting instrument up and down in the thickness direction of the gel, which has the advantage of facilitating the cutting operation. However, the cutting instrument is not limited to such a pipe-shaped one, and may be one that is cut out using a scalpel.
【００１３】図４は第２の実施例における抽出器具の部
分を表わしたものである。この抽出器具４０は、図１，
２に示した第１の実施例の切出し器具８に代えて、切出
し器具８の位置に設置されて使用されるものである。抽
出器具４０はＺ駆動部１２に中空のパイプ４２が設けら
れたものであり、そのパイプ４２にはゲル又は電解液４
４が充填されている。抽出を電気泳動的に実行するため
に、図には現れていないが、電源装置が設けられてお
り、その電源装置からパイプ４２内のゲル又は電解液４
４の上端部に＋電位、ガラス板６上のゲル４に−電位が
印加される。FIG. 4 shows a part of the extraction device according to the second embodiment. This extraction device 40 is shown in FIG.
Instead of the cutting instrument 8 of the first embodiment shown in FIG. 2, the cutting instrument 8 is installed and used at the position of the cutting instrument 8. The extraction device 40 is a Z drive unit 12 provided with a hollow pipe 42, and the pipe 42 has a gel or electrolyte 4
4 is filled. In order to carry out the extraction electrophoretically, a power supply, not shown, is provided, from which the gel or electrolyte 4 in the pipe 42 is provided.
A + potential is applied to the upper end of 4, and a −potential is applied to the gel 4 on the glass plate 6.
【００１４】目的とする泳動バンドを抽出するときに
は、第１の実施例と同様に、抽出器具４の先端が抽出す
べき泳動バンドのゲル部分４ａに接する位置まで移動さ
せられ、パイプ４２中のゲル又は電解液４４が＋電位、
ゲル４が−電位になるように電圧が印加される。これに
より、ゲル部分４ａの泳動ハンドが電気泳動によってパ
イプ４２中のゲル又は電解液４４へ抽出される。When the target electrophoretic band is extracted, the tip of the extraction instrument 4 is moved to a position in contact with the gel portion 4a of the electrophoretic band to be extracted, and the gel in the pipe 42 is extracted, as in the first embodiment. Alternatively, the electrolytic solution 44 is + potential,
A voltage is applied so that the gel 4 has a negative potential. As a result, the electrophoretic hand of the gel portion 4a is extracted into the gel in the pipe 42 or the electrolytic solution 44 by electrophoresis.
【００１５】抽出後の泳動バンドは、パイプ４２中のゲ
ル又は電解液４４とともに図２の場合と同様に容器２４
へ吐出される。ゲル４の泳動バンドを抽出する位置の指
定や抽出器具４０の移動制御は、第１の実施例の場合と
同じである。The electrophoretic band after the extraction, together with the gel in the pipe 42 or the electrolytic solution 44, is the same as in the case of FIG.
Is discharged to. The designation of the extraction position of the migration band of the gel 4 and the movement control of the extraction device 40 are the same as those in the first embodiment.
【００１６】実施例のゲルは別の電気泳動装置により試
料の電気泳動を完了したものを基台２上に載置するもの
を例示しているが、泳動前のゲル４を基台２上に載置す
る場合には、ゲル４の両端に泳動電圧を印加する電源を
設け、基台２上で電気泳動を行なわせた後に、上記の動
作にしたがって所定領域の切出しを行なうようにすれば
よい。As the gel of the embodiment, the gel of which the sample has been electrophoresed by another electrophoresis apparatus is placed on the base 2, but the gel 4 before the electrophoresis is placed on the base 2. When the gel 4 is placed, a power supply for applying an electrophoretic voltage is provided at both ends of the gel 4, electrophoresis is performed on the base 2, and then a predetermined region is cut out in accordance with the above operation. .
【００１７】[0017]
【発明の効果】本発明では、基台２上に載置され、電気
泳動の完了した状態のゲルの泳動パターンを光学的に認
識し、そのままの状態で、作業アーム機構によって切出
し器具又は抽出器具をＸ，Ｙ方向とＺ方向に移動させ
て、ゲルの所定部分を切り出し又は抽出するようにした
ので、泳動パターンの認識から切出し又は抽出完了まで
ゲルを移動させることなく一連の作業として行なうこと
ができ、切出し又は抽出動作が容易で、自動化も可能に
なる。According to the present invention, the electrophoretic pattern of the gel, which is placed on the base 2 and in which electrophoresis has been completed, is optically recognized, and the cutting arm or the extraction tool is used as it is by the working arm mechanism. Was moved in the X, Y and Z directions to cut out or extract a predetermined part of the gel. Therefore, a series of operations can be performed without moving the gel from the recognition of the migration pattern to the completion of cutting or extraction. It is possible, the cutting or extracting operation is easy, and automation is possible.
【図１】一実施例を表わしたものであり、（Ａ）は平面
図、（Ｂ）は分取制御部などをブロック図で表わした正
面図である。FIG. 1 shows an embodiment, (A) is a plan view, and (B) is a front view showing a fractionation control unit and the like in a block diagram.
【図２】切出し部を拡大して示す図であり、（Ａ）は概
略正面断面図、（Ｂ）は切出し器具に詰め込んだゲル部
分を容器に取り出す状態を示す断面図である。2A and 2B are enlarged views of a cutout portion, FIG. 2A is a schematic front cross-sectional view, and FIG. 2B is a cross-sectional view showing a state in which a gel portion packed in a cutout device is taken out into a container.
【図３】表示部に表示される画像データと切出し領域を
指定する方法を示す図であり、（Ａ）は表示部の画像デ
ータを示す図、（Ｂ）は濃度信号（右側）と切出し領域
（左側）を示す図である。3A and 3B are diagrams showing a method of designating image data displayed on a display unit and a cutout region, FIG. 3A is a diagram showing image data on the display unit, and FIG. 3B is a density signal (right side) and cutout region. It is a figure which shows (left side).
【図４】第２の実施例における抽出器具を示す概略正面
断面図である。FIG. 4 is a schematic front sectional view showing an extraction device according to a second embodiment.
【符号の説明】 ２ 基台 ４ ゲル ６ ガラス板 ８ 切出し器具 １０ 作業アーム機構 １２ Ｚ駆動部 １６ Ｘ駆動部 ２０ Ｙ駆動部 ３０ 光源 ３２ 光検出器 ３４ 分取制御部 ３６ 表示部 ４０ 抽出器具[Explanation of symbols] 2 bases 4 gel 6 glass plates 8 cutting equipment 10 Working arm mechanism 12 Z drive 16 X drive 20 Y drive 30 light sources 32 photo detector 34 Preparative control unit 36 Display 40 Extractor
───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 27/447 G01N 1/10 - 1/34 G01N 35/00 - 35/08 ─────────────────────────────────────────────────── ─── Continuation of front page (58) Fields surveyed (Int.Cl. 7 , DB name) G01N 27/447 G01N 1/10-1/34 G01N 35/00-35/08
Claims (2)
料の分離が完了し、そのスラブ状ゲルを前後から挟んで
支持していた２枚の支持板のうちの少なくとも一方の支
持板が剥がされた状態で露出したゲルが上向きになるよ
うに載置される基台と、 その基台の上方に配置され基台上に載置されたゲルの所
定部分を切り出す切出し器具と、 その切出し器具を基台の面に平行なＸ，Ｙ方向と基台の
面に垂直なＺ方向とに移動させてその切出し器具による
ゲルの所定部分の切出し動作を行なう作業アーム機構
と、 基台上に載置されたゲルの泳動パターンを光学的に検出
する泳動パターン検出手段と、 泳動パターン検出手段の検出結果に基づいて自動的に又
は手動入力により定められた所定の泳動バンドのゲルを
切り取るように、前記作業アーム機構を制御する分取制
御部と、を備えたことを特徴とするサンプル分取装置。1. Separation of a sample by electrophoresis using a slab-like gel is completed, and at least one of the two support plates that sandwich and support the slab-like gel is peeled off. The base is placed so that the exposed gel faces upward, the cutting tool that is placed above the base and cuts out a predetermined portion of the gel that is placed on the base, and the cutting tool. A work arm mechanism that moves the X and Y directions parallel to the surface of the base and Z directions perpendicular to the surface of the base to perform a cutting operation of a predetermined portion of the gel by the cutting tool, and a working arm mechanism that is placed on the base. The electrophoretic pattern detecting means for optically detecting the electrophoretic pattern of the formed gel, and the gel of a predetermined electrophoretic band determined automatically or manually based on the detection result of the electrophoretic pattern detecting means is cut out, Working arm machine Samples winding device being characterized in that and a preparative controller for controlling.
料の分離が完了し、そのスラブ状ゲルを前後から挟んで
支持していた２枚の支持板のうちの少なくとも一方の支
持板が剥がされた状態で露出したゲルが上向きになるよ
うに載置される基台と、 その基台の上方に配置され基台上に載置されたゲルの所
定部分の泳動バンドを電気泳動的に抽出する抽出器具
と、 その抽出器具を基台の面に平行なＸ，Ｙ方向と基台の面
に垂直なＺ方向とに移動させてその抽出器具によるゲル
の所定部分の泳動バンドの抽出動作を行なう作業アーム
機構と、 基台上に載置されたゲルの泳動パターンを光学的に検出
する泳動パターン検出手段と、 泳動パターン検出手段の検出結果に基づいて自動的に又
は手動入力により定められた所定の泳動バンドを抽出す
るように、前記作業アーム機構を制御する分取制御部
と、を備えたことを特徴とするサンプル分取装置。2. The separation of the sample by electrophoresis using the slab-like gel is completed, and at least one of the two support plates sandwiching and supporting the slab-like gel from the front and back is peeled off. The electrophoretic extraction of the pedestal placed on the pedestal with the exposed gel facing upward and the electrophoretic band of the predetermined part of the gel placed on the pedestal placed above the pedestal Extraction device and the extraction device is moved in the X and Y directions parallel to the surface of the base and in the Z direction perpendicular to the surface of the base to perform the extraction operation of the migration band of a predetermined portion of the gel by the extraction device. Working arm mechanism, electrophoretic pattern detection means for optically detecting the electrophoretic pattern of the gel placed on the base, and a predetermined value determined automatically or by manual input based on the detection result of the electrophoretic pattern detection means. I will extract the migration band of , The preparative samples, characterized in that it and a preparative control unit for controlling the working arm mechanism device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27640095A JP3381484B2 (en) | 1995-04-26 | 1995-09-29 | Sample separation device by gel electrophoresis |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12721295 | 1995-04-26 | ||
| JP7-127212 | 1995-04-26 | ||
| JP27640095A JP3381484B2 (en) | 1995-04-26 | 1995-09-29 | Sample separation device by gel electrophoresis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0915206A JPH0915206A (en) | 1997-01-17 |
| JP3381484B2 true JP3381484B2 (en) | 2003-02-24 |
Family
ID=26463222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP27640095A Expired - Fee Related JP3381484B2 (en) | 1995-04-26 | 1995-09-29 | Sample separation device by gel electrophoresis |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3381484B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101339770B1 (en) * | 2011-08-17 | 2013-12-11 | 충북대학교 산학협력단 | Cutting tool for band extrcting from gelationous material and band rextrcting method using the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPP930099A0 (en) * | 1999-03-19 | 1999-04-15 | Campbell Corporation Pty Ltd | Improvements in apparatus and method for removing samples |
| JP2002048765A (en) * | 2000-08-03 | 2002-02-15 | Masabumi Youda | Sample introduction device for horizontal plane type gel electrophorestic device, horizontal plane type gel electrophorestic system, its sample introduction method and its sample collection method |
| JP3660907B2 (en) * | 2002-01-29 | 2005-06-15 | アロカ株式会社 | Sample plate processing equipment |
| US7247275B2 (en) | 2004-06-21 | 2007-07-24 | Jeremy Scot Caldwell | Gel extraction device |
| JP4619198B2 (en) * | 2005-05-27 | 2011-01-26 | パナソニック株式会社 | Electrophoresis system |
| JP4619202B2 (en) * | 2005-06-01 | 2011-01-26 | パナソニック株式会社 | Electrophoresis system |
| JP4632876B2 (en) * | 2005-06-24 | 2011-02-16 | パナソニック株式会社 | Electrophoresis device |
| EP1910511A1 (en) * | 2005-08-01 | 2008-04-16 | Jae Gyeong Jeong | Recovery system of dna and rna or protein fragments with agarose gel or polyacrylamide gel |
| WO2007073367A1 (en) | 2005-12-20 | 2007-06-28 | Jeremy Caldwell | Tool for extracting electrophoretic sample |
| JP4617394B1 (en) * | 2010-02-12 | 2011-01-26 | シーシーアイ株式会社 | Fireproof closure |
| EP2587245A4 (en) * | 2010-06-22 | 2017-08-30 | Universal Bio Research Co., Ltd. | Device for trapping biologically-relevant substances and system for collecting biologically-relevant substances |
| JP6027724B2 (en) * | 2011-04-26 | 2016-11-16 | 株式会社住化分析センター | Inspection method for fine particles |
| JP5594501B1 (en) | 2013-04-11 | 2014-09-24 | 株式会社昇竜建設 | Gel plate fragmentation / dispensing device and fragmentation / dispensing method |
-
1995
- 1995-09-29 JP JP27640095A patent/JP3381484B2/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101339770B1 (en) * | 2011-08-17 | 2013-12-11 | 충북대학교 산학협력단 | Cutting tool for band extrcting from gelationous material and band rextrcting method using the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0915206A (en) | 1997-01-17 |
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