JP3381239B2 - Bag for lymphocyte separation - Google Patents
Bag for lymphocyte separationInfo
- Publication number
- JP3381239B2 JP3381239B2 JP28697792A JP28697792A JP3381239B2 JP 3381239 B2 JP3381239 B2 JP 3381239B2 JP 28697792 A JP28697792 A JP 28697792A JP 28697792 A JP28697792 A JP 28697792A JP 3381239 B2 JP3381239 B2 JP 3381239B2
- Authority
- JP
- Japan
- Prior art keywords
- flexible tube
- bag body
- bag
- blood
- inlet port
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Centrifugal Separators (AREA)
- Medical Preparation Storing Or Oral Administration Devices (AREA)
- External Artificial Organs (AREA)
Description
【発明の詳細な説明】
【０００１】
【産業上の利用分野】本発明はリンパ球分離用バツグに
関するものである。
【０００２】
【従来の技術】近年、習慣流産患者に対して、夫リンパ
球を用いた免疫療法が行われるようになった。これは免
疫学的には腎臓移植等の前に行われるｄｏｎｏｒ ｓｐ
ｅｃｉｆｉｃｔｒａｎｓｆｕｓｉｏｎ（ＤＳＴ）に非常
によく似ていると考えられている。
【０００３】免疫療法は、日本産科婦人科学会雑誌４３
巻２号によれば、次のように行われる。夫のヘパリン加
末梢血３０ｍｌよりフイコール（Ｆｉｃｏｌｌ）比重遠
心法でリンパ球を分離し、３．５〜５．０×１０7 個／
ｍｌになるように滅菌生理食塩水で濃度調製し、これを
妻の上腕と前腕に６〜８カ所皮内接種する。これを２〜
４週間間隔で４回施行される。
【０００４】ここでフイコールとはシヨ糖とエピクロロ
ヒドリンを共重合した分子量４０万の水溶性の合成品で
Ｐｈａｒｍａｃｉａ社の商品名であり、溶かすと高密度
・低粘度の溶液となる。濃度５０％（ｗ／ｖ）、密度
１．２ｇ／ｍｌまでの範囲で使用され、生物材料と等し
い浸透圧をつくることにより、細胞・ウイルス・細胞小
器官・膜結合顆粒などを分離する目的で、連続及び不連
続密度勾配遠心法などに用いられる。フイコール自身凝
集作用があるので赤血球及び顆粒球をそれぞれ凝集して
先に沈降させ、血液成分分離をより効果的にするもので
ある。
【０００５】ヘパリン加末梢血などから白血球を分離す
るために、従来用いられている方法は例えば以下のとお
りである。試験管に比重分離液である、株式会社免疫生
物研究所から発売されているリンホセパールＩ３ｍｌを
入れ、リン酸緩衝化食塩水（以下、ＰＢＳと言う。）な
どで２倍に希釈した血液数ｍｌを静かに重層する。次に
１８００ｒｐｍ（４００×ｇ）で室温下３０分間遠心す
る。試験管内では上層から血漿・リンパ球層・比重分離
液・赤血球の順に分離され、リンパ球層を容量２ｍｌ程
度のシリンジで取り出し別の遠心管にとり、ＰＢＳで３
回洗浄して使用される。
【０００６】
【発明が解決しようとする課題】もともと血液成分には
原則として接触しないことが作業者の安全管理上必要だ
が、従来の方法であると、密閉状態で血液成分を分離し
ないので、操作中にリンパ球以外の血液成分に接触する
心配があった。
【０００７】本発明はリンパ球分離において、操作が簡
便で、作業者の安全管理上好ましく使用できるバツグを
提供するものである。
【０００８】
【課題を解決するための手段】本発明は、周縁部が融着
または高周波シールされている透明又は半透明の合成樹
脂からなるバツグ本体において、バツグ本体の上部に入
口ポート及び排出口があり、入口ポートは血液又は薬液
が投入される可撓性チユーブが接続され、排出口のバツ
グ本体の外部の側に分岐管を介して２個の移行バツグに
連結する可撓性チユーブが接続され、排出口のバツグ本
体の内部の側にバツグ本体の内部空間の下部まで達する
可撓性チユーブが接続されていることを特徴とするリン
パ球分離用バツグを要旨とする。
【０００９】本発明のバツグ本体は、容量が３０〜５０
ｍｌが適当であり、材質は塩化ビニルやポリオレフイン
でできている。本発明の可撓性チユーブはバツグ本体と
同じ材質である必要はないが、合成樹脂製である。
【００１０】
【作用】本発明のバツグは、透明又は半透明の材質から
なるが、これは血液を遠心分離した後、バツグの外側か
ら眺めた場合、各成分ごとの存在を確認しやすくしたも
のである。
【００１１】本発明ではバツグ本体に必要な入口ポート
及び排出口をバツグ本体の上部に位置するように設け、
このような位置に配列させることにより、バツグの製造
を容易にし、遠心分離の操作が容易になるようにしてあ
る。
【００１２】本発明では入口ポートから血液又は薬液を
投入する。それにはシリンジ・可撓性チユーブを用いて
行う。
【００１３】本発明のバツグ本体は可撓性チユーブを通
じて、２個の移行バツグに連結されている。このうち１
個の移行バツグには遠心分離後の赤血球・フイコール液
が収納され、もう１個の移行バツグには分離された白血
球が収納される。
【００１４】本発明において、排出口のバツグ本体の内
部の側に接続されている可撓性チユーブはバツグ本体の
内部空間の下部まで達している。これは遠心分離後バツ
グ本体の下部に集まった血液成分を取り出すために必要
である。
【００１５】
【発明の効果】本発明は以上のような構成と作用を有す
るので、本発明のバツグを使用すると、白血球分離の操
作において、リンパ球以外の血液成分に作業者が接触す
る心配がなく、操作も簡便で、作業者の安全管理上好ま
しいと言える。
【００１６】
【実施例】以下に、本発明のリンパ球分離用バツグを用
いた場合の手技例の説明図（図１）に基づいて、具体的
に説明する。バツグ本体２は容量が４０ｍｌで、周縁部
１が高周波シールされている塩化ビニル製とした。
【００１７】入口ポート３からシリンジ（図示されてい
ない。）を用いてリンパ球比重分離液であるフイコール
液を入れた。次に入口ポート３からヘパリン血を界面を
乱さないように静かに入れた。
【００１８】移行バツグ７、８が連結されたままの上記
バツグ本体２を、室温で４００×ｇの強さで３０分間遠
心分離を行った。遠心後はバツグ本体２の上部に血漿が
集まり、バツグ本体２の中央部にリンパ球が集まり、赤
血球はフイコール液を介してリンパ球の下に集まった。
【００１９】バツグ本体２を圧縮し、バツグ本体の内部
空間の下部で開口している可撓性チユーブ１１から赤血
球・フイコール液を移行バツグ７に移送した。同様にし
てリンパ球を移行バツグ８に移送した。分岐管６近辺で
チユーブをシール・切断した。
【００２０】リンパ球を洗浄するために、移行バツグ８
にシリンジを用いて、可撓性チユーブを通じて、ＰＢＳ
を投入し、遠心分離を行い、上層のＰＢＳを廃棄した。
これを３回繰り返し、免疫療法用リンパ球を得た。Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a bag for separating lymphocytes. [0002] In recent years, immunotherapy using a husband's lymphocyte has been performed for a habitual abortion patient. This is done immunologically by donor sp
It is believed to be very similar to effectivetransfusion (DST). [0003] Immunotherapy is a Japanese journal of obstetrics and gynecology 43
According to Vol. 2, the process is performed as follows. Lymphocytes were separated from Fischer's heparinized peripheral blood (30 ml) by Ficoll specific gravity centrifugation, and 3.5 to 5.0 × 10 7 cells /
The concentration is adjusted with sterile physiological saline to a volume of 6 ml, and this is intradermally inoculated into the upper arm and forearm of the wife at 6 to 8 sites. This is 2
It is administered four times every four weeks. [0004] Here, ficoll is a water-soluble synthetic product having a molecular weight of 400,000 which is obtained by copolymerizing sucrose and epichlorohydrin, and is a trade name of Pharmacia. When dissolved, it becomes a solution of high density and low viscosity. It is used at a concentration of 50% (w / v) and a density of up to 1.2 g / ml, and is used to separate cells, viruses, organelles, membrane-bound granules, etc. by creating an osmotic pressure equal to that of biological material. , Continuous and discontinuous density gradient centrifugation. Since ficoll itself has an agglutinating action, red blood cells and granulocytes are each agglutinated and sedimented first to make blood component separation more effective. [0005] A method conventionally used for separating leukocytes from heparinized peripheral blood is as follows, for example. In a test tube, 3 ml of lymphosepal I, which is a specific gravity separation solution sold by the Institute for Immunity and Biological Sciences, is placed, and several ml of blood diluted twice with phosphate buffered saline (hereinafter referred to as PBS) or the like is added. Layer gently. Next, centrifuge at 1800 rpm (400 × g) at room temperature for 30 minutes. In the test tube, the plasma, lymphocyte layer, specific gravity separation solution, and red blood cells are separated in this order from the upper layer, and the lymphocyte layer is taken out with a syringe having a capacity of about 2 ml, taken into another centrifuge tube, and washed with PBS.
Used after washing. [0006] It is originally necessary for the safety management of workers not to come into contact with blood components in principle, but with the conventional method, blood components are not separated in a hermetically sealed state. There was a concern that it would come into contact with blood components other than lymphocytes. [0007] The present invention provides a bag which is easy to operate in lymphocyte separation and can be preferably used for safety management of workers. SUMMARY OF THE INVENTION The present invention relates to a bag body made of a transparent or translucent synthetic resin whose peripheral portion is sealed by fusion or high-frequency sealing. The inlet port is connected to a flexible tube into which blood or a drug solution is injected, and a flexible tube connected to two transition bags via a branch pipe is connected to the outlet outside the bag body. The gist of the present invention provides a bag for separating lymphocytes, wherein a flexible tube reaching the lower part of the internal space of the bag body is connected to the inside of the bag body at the outlet. The bag body of the present invention has a capacity of 30 to 50.
ml is appropriate and the material is made of vinyl chloride or polyolefin. The flexible tube of the present invention need not be made of the same material as the bag body, but is made of synthetic resin. The bag of the present invention is made of a transparent or translucent material, which makes it easy to confirm the presence of each component when blood is centrifuged and viewed from the outside of the bag. It is. In the present invention, an inlet port and a discharge port required for the bag body are provided so as to be located at an upper portion of the bag body.
By arranging at such positions, the manufacture of the bag is facilitated, and the operation of centrifugation is facilitated. In the present invention, blood or a drug solution is introduced from the inlet port. This is done using a syringe / flexible tube. The bag body of the present invention is connected to two transition bags through a flexible tube. One of these
One transfer bag contains the red blood cells and Ficoll solution after centrifugation, and the other transfer bag contains the separated white blood cells. In the present invention, the flexible tube connected to the inside of the bag body at the outlet extends to the lower part of the internal space of the bag body. This is necessary to remove blood components collected at the lower part of the bag body after centrifugation. Since the present invention has the above-described structure and operation, the use of the bag of the present invention may cause a worker to come into contact with blood components other than lymphocytes in the operation of separating white blood cells. In addition, the operation is simple and it can be said that it is preferable in terms of worker safety management. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a specific description will be given based on an explanatory view (FIG. 1) of a procedure example using the bag for separating lymphocytes of the present invention. The bag body 2 was made of vinyl chloride having a capacity of 40 ml and a high frequency seal at the peripheral portion 1. A ficoll solution, which is a specific separation liquid of lymphocytes, was introduced from the inlet port 3 using a syringe (not shown). Next, heparin blood was gently introduced from the inlet port 3 so as not to disturb the interface. The bag main body 2 with the transfer bags 7 and 8 connected was centrifuged at room temperature at 400 × g for 30 minutes. After the centrifugation, plasma was collected at the upper part of the bag body 2, lymphocytes were collected at the center of the bag body 2, and red blood cells were collected under the lymphocytes via the Ficoll solution. The bag body 2 was compressed, and the red blood cell / ficoll solution was transferred to the transfer bag 7 from the flexible tube 11 opened at the lower part of the internal space of the bag body. Similarly, lymphocytes were transferred to the transfer bag 8. The tube was sealed and cut near the branch pipe 6. To wash lymphocytes, transfer bag 8
Using a syringe, pass through a flexible tube, PBS
, And centrifuged, and the upper layer of PBS was discarded.
This was repeated three times to obtain immunotherapy lymphocytes.
【図面の簡単な説明】
【図１】本発明のリンパ球分離用バツグを用いた場合の
手技例の説明図である。
【符号の説明】
１ 周縁部
２ バツグ本体
３ 入口ポート
４ 排出口
５ 可撓性チユーブ
６ 分岐管
７ 移行バツグ
８ 移行バツグ
９ 可撓性チユーブ
１０ バツグ本体の内部
１１ 可撓性チユーブBRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is an explanatory diagram of an example of a technique using the bag for lymphocyte separation of the present invention. [Description of Signs] 1 Peripheral part 2 Bag body 3 Inlet port 4 Outlet 5 Flexible tube 6 Branch pipe 7 Transition bag 8 Transition bag 9 Flexible tube 10 Inside of bag body 11 Flexible tube
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 平３−65177（ＪＰ，Ａ) 特開 昭63−132658（ＪＰ，Ａ) 特開 平５−76581（ＪＰ，Ａ) 特開 平１−230521（ＪＰ，Ａ) 特開 昭64−22258（ＪＰ，Ａ) 特開 昭62−217971（ＪＰ，Ａ) 特開 昭59−155262（ＪＰ，Ａ) 特開 平６−30986（ＪＰ，Ａ) 実開 昭60−42370（ＪＰ，Ｕ) 実開 平２−15149（ＪＰ，Ｕ) 実開 昭58−18552（ＪＰ，Ｕ) 実開 昭62−59034（ＪＰ，Ｕ) 国際公開91／15182（ＷＯ，Ａ１) (58)調査した分野(Int.Cl.7，ＤＢ名) A61J 1/10 ──────────────────────────────────────────────────続 き Continuation of front page (56) References JP-A-3-65177 (JP, A) JP-A-63-132658 (JP, A) JP-A-5-76581 (JP, A) JP-A-1- 230521 (JP, A) JP-A-64-22258 (JP, A) JP-A-62-217971 (JP, A) JP-A-59-155262 (JP, A) JP-A-6-30986 (JP, A) Japanese Utility Model Application Showa 60-42370 (JP, U) Japanese Utility Model Application Hei 2-15149 (JP, U) Japanese Utility Model Application 58-18552 (JP, U) Japanese Utility Model Application Showa 62-59034 (JP, U) International Publication 91/15182 (Japanese) (WO, A1) (58) Field surveyed (Int. Cl. 7 , DB name) A61J 1/10
Claims (1)
いる透明又は半透明の合成樹脂からなるバッグ本体と、
バッグ本体の上部に設けられた入り口ポート及び排出口
と、入口ポートに接続された血液又は薬液が投入される
可撓性チューブと、排出口のバッグ本体の外部の側に連
結された可撓性チューブおよび該可撓性チューブから分
岐管を介して分岐した可撓性チューブと、該可撓性チュ
ーブおよび分岐した可撓性チューブに接続された２個の
移行バッグと、排出口のバッグ本体の内部の側に接続さ
れたバッグ本体の内部空間の下部まで達する可撓性チュ
ーブとからなることを特徴とするリンパ球分離用バッ
グ。(57) [Claim 1] A bag body made of a transparent or translucent synthetic resin whose peripheral portion is fused or high-frequency sealed,
Inlet port and outlet provided at the top of the bag body
And a flexible tube connected to the inlet port , into which blood or a drug solution is injected, and a discharge port connected to the outside of the bag body.
A flexible tube connected to the flexible tube, a flexible tube branched from the flexible tube via a branch tube, and the flexible tube.
Over Bed and branched and two transition bags connected to the flexible tubes, is connected to the inner side of the bag body of the outlet
Lymphocyte separation bag, characterized in that it consists to reach the flexible tube to the bottom of the interior space of the bag body that was.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28697792A JP3381239B2 (en) | 1992-09-30 | 1992-09-30 | Bag for lymphocyte separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28697792A JP3381239B2 (en) | 1992-09-30 | 1992-09-30 | Bag for lymphocyte separation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06114290A JPH06114290A (en) | 1994-04-26 |
| JP3381239B2 true JP3381239B2 (en) | 2003-02-24 |
Family
ID=17711418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP28697792A Expired - Lifetime JP3381239B2 (en) | 1992-09-30 | 1992-09-30 | Bag for lymphocyte separation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3381239B2 (en) |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4007871A (en) * | 1975-11-13 | 1977-02-15 | International Business Machines Corporation | Centrifuge fluid container |
| JPS5638851U (en) * | 1979-08-31 | 1981-04-11 | ||
| JPS56151663U (en) * | 1980-04-08 | 1981-11-13 | ||
| JPS5795247U (en) * | 1980-12-01 | 1982-06-11 | ||
| JPS5818552U (en) * | 1981-07-29 | 1983-02-04 | 株式会社クラレ | Fluid retention bag |
| GB2134789A (en) * | 1983-02-11 | 1984-08-22 | Bard Ltd | Adjustable length tube and seal therefor |
| JPS6042370U (en) * | 1983-09-01 | 1985-03-25 | テルモ株式会社 | liquid separation tube |
| JPS61121830U (en) * | 1985-01-17 | 1986-07-31 | ||
| JPS6259034U (en) * | 1985-10-02 | 1987-04-13 | ||
| JPS62217971A (en) * | 1986-03-19 | 1987-09-25 | 日本赤十字社 | Single needle type serum sampler |
| JPS63132658A (en) * | 1986-11-25 | 1988-06-04 | テルモ株式会社 | Blood preserving container |
| JPS6422258A (en) * | 1987-07-17 | 1989-01-25 | Ube Industries | Blood bag |
| JPH0630199Y2 (en) * | 1988-07-14 | 1994-08-17 | 川澄化学工業株式会社 | Body fluid storage bag |
| JPH01230521A (en) * | 1989-01-13 | 1989-09-14 | Nippon Sekijiyuujishiya | Separation of blood component |
| JP2726708B2 (en) * | 1989-08-01 | 1998-03-11 | 株式会社ニッショー | Cell culture bag |
| SE9001271D0 (en) * | 1990-04-06 | 1990-04-06 | Eric Westberg | BLOOD PASSE FOR APPLICATION FOR SEPARATION OF BLOOD IN COMPONENTS |
| JP3221701B2 (en) * | 1991-09-24 | 2001-10-22 | テルモ株式会社 | Body fluid bag |
| JPH0630986A (en) * | 1992-07-17 | 1994-02-08 | Nissho Corp | Blood bag set |
-
1992
- 1992-09-30 JP JP28697792A patent/JP3381239B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06114290A (en) | 1994-04-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0349188B1 (en) | Method for separating blood into blood components, and blood components separator unit | |
| JPH01502560A (en) | Plasma transfer method and device | |
| EP1683857B1 (en) | Method of preparing cell concentrate and cell composition | |
| JPH01320064A (en) | Blood component separating system | |
| JP3381239B2 (en) | Bag for lymphocyte separation | |
| AU665140B2 (en) | Method for filtering a body fluid using rinse chamber bag | |
| JP2750918B2 (en) | Red blood cell storage bag | |
| JP3208132B2 (en) | Blood component separation method | |
| JPH09206356A (en) | Blood bag and blood component separation method | |
| JPH0741066B2 (en) | Blood component separation bag and blood component separation method | |
| JP3065727B2 (en) | Mononuclear cell separation tube and mononuclear cell separation method | |
| JPS5854130B2 (en) | Leukocyte separation method | |
| JPS6326089B2 (en) | ||
| JPH04236959A (en) | Blood component separating system | |
| JPS5914008B2 (en) | Lymphocyte collection method and device | |
| JPH09276368A (en) | Blood bag set | |
| JPS5854128B2 (en) | Lymphocyte isolation method and device | |
| JPH09168585A (en) | Blood bag system | |
| JPS5854131B2 (en) | How to isolate leukocytes | |
| JPH0531177A (en) | Blood collecting appliance | |
| CN116942938A (en) | Whole blood collection and separation method and device | |
| JPS6328412B2 (en) | ||
| de Wit et al. | A dextran agglomeration method for the preparation in a closed plastic bag system of leucocyte poor blood for transfusion | |
| JPH09168574A (en) | Method of separating blood components | |
| JPH11206875A (en) | Filter set and recovery of blood component using the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081220 Year of fee payment: 6 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20111220 Year of fee payment: 9 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20111220 Year of fee payment: 9 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20121220 Year of fee payment: 10 |
|
| EXPY | Cancellation because of completion of term | ||
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20121220 Year of fee payment: 10 |