JP3356212B2 - Index matching transparent film and cell using index matching transparent film - Google Patents
Index matching transparent film and cell using index matching transparent filmInfo
- Publication number
- JP3356212B2 JP3356212B2 JP2001015558A JP2001015558A JP3356212B2 JP 3356212 B2 JP3356212 B2 JP 3356212B2 JP 2001015558 A JP2001015558 A JP 2001015558A JP 2001015558 A JP2001015558 A JP 2001015558A JP 3356212 B2 JP3356212 B2 JP 3356212B2
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- Japan
- Prior art keywords
- refractive index
- transparent
- metal thin
- index matching
- thin film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Description
【０００１】[0001]
【発明の属する技術分野】本発明は、光学系を用いて被
測定溶液中の特定物質を定量あるいは定性的に測定する
表面プラズモン共鳴現象を利用した測定に用いる屈折率
整合透明フィルムに関し、より詳細には、被測定物に接
した金属薄膜における表面プラズモン共鳴現象を利用
し、液体やガスなどの被測定物の屈折率変化を検知し、
これらの定性・定量などの測定を行う装置に用いる屈折
率整合透明フィルムおよび屈折率整合透明フィルムを利
用したセルに関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a refractive index matching transparent film used for measurement using a surface plasmon resonance phenomenon for quantitatively or qualitatively measuring a specific substance in a solution to be measured using an optical system. Utilizes the surface plasmon resonance phenomenon in a metal thin film in contact with the object to detect the change in the refractive index of the object to be measured such as liquid or gas,
The present invention relates to a refractive index matching transparent film used in an apparatus for performing such qualitative and quantitative measurements and a cell using the refractive index matching transparent film.
【０００２】[0002]
【従来の技術】従来より、科学プロセス計測や環境計
測、また臨床検査などにおいて、呈色反応や免疫反応を
利用した測定が行われている。しかしながら、これらの
測定方法では、被測定物をサンプル抽出する必要がある
ほか、煩雑な操作や標識物質を必要とするなどの問題が
あった。これらに対し、標識物質を必要とすることな
く、高感度で被測定物中の化学物質の定性・定量測定が
可能な測定手法として、光励起表面プラズモン共鳴現象
（Surface Plasmon Resonance）を利用したセンサを用
いる手法が提案され、また実用化されている。2. Description of the Related Art Hitherto, measurement utilizing a color reaction or an immune reaction has been performed in scientific process measurement, environmental measurement, clinical examination, and the like. However, in these measurement methods, there is a problem that a sample to be measured needs to be sampled, and a complicated operation and a labeling substance are required. On the other hand, a sensor that uses photo-excited surface plasmon resonance (Surface Plasmon Resonance) as a measurement method that can perform qualitative and quantitative measurements of chemical substances in an object with high sensitivity without requiring a labeling substance The method used has been proposed and put into practical use.
【０００３】従来のＳＰＲ現象測定装置は、図５に示す
ように、光源５０１、Ｐ偏光光のみを通過させる偏光板
５０２およびレンズ５０３、プリズム５０４、光電子検
出器５０５、金属薄膜５０６、プリズム５０４と同じ屈
折率のガラスや高分子透明体の透明基板５０７、および
透明基板５０７をプリズム５０４に張り付けるためのマ
ッチングオイル５０８とから構成されている。As shown in FIG. 5, a conventional SPR phenomenon measuring apparatus includes a light source 501, a polarizing plate 502 and a lens 503, a prism 504, a photoelectron detector 505, a metal thin film 506, and a prism 504 that allow only P-polarized light to pass therethrough. The transparent substrate 507 is made of glass or a polymer transparent body having the same refractive index, and a matching oil 508 for attaching the transparent substrate 507 to the prism 504.
【０００４】図５のＳＰＲ現像測定装置の動作について
説明すると、まず、光源５０１から発した光を偏光板５
０２，レンズ５０３を通過させる。つぎに、通過させた
光をある入射角θをもってプリズム５０４に入射させ、
入射させた光を被測定物が配置された金属薄膜５０６に
照射する。この結果、金属薄膜５０６で反射した光を、
プリズム５０４を通して光電子検出器５０５で検出す
る。The operation of the SPR development measuring apparatus shown in FIG. 5 will be described. First, the light emitted from the light source 501 is applied to the polarizing plate 5.
02, pass through the lens 503. Next, the transmitted light is incident on the prism 504 at a certain incident angle θ,
The incident light is applied to the metal thin film 506 on which the object to be measured is arranged. As a result, the light reflected by the metal thin film 506 is
The light is detected by a photoelectron detector 505 through a prism 504.
【０００５】光源５０１から発した光は、金属薄膜５０
６の反射面でエバネッセント波となる。この波数は、つ
ぎの数１により表される。[0005] Light emitted from the light source 501 is
An evanescent wave is formed on the reflecting surface 6. This wave number is represented by the following Equation 1.
【０００６】[0006]
【数１】 (Equation 1)
【０００７】上記数１において、ｋpは入射光の波数、
ｎpはプリズム５０４の屈折率、θは入射角である。In the above equation 1, k p is the wave number of the incident light,
n p is the refractive index of the prism 504, and θ is the incident angle.
【０００８】このとき、金属薄膜５０６の反射面では、
表面プラズモン波が生じ、この波数は、以下の数２によ
り表される。At this time, on the reflection surface of the metal thin film 506,
A surface plasmon wave is generated, and this wave number is represented by the following Equation 2.
【０００９】[0009]
【数２】 (Equation 2)
【００１０】上記数２において、ｃは光速、ωは角振動
数、εは金属薄膜５０６の誘電率、ｎは金属薄膜５０６
上に配置された被測定物の屈折率である。In the above equation (2), c is the speed of light, ω is the angular frequency, ε is the dielectric constant of the metal thin film 506, and n is the metal thin film 506.
This is the refractive index of the object placed above.
【００１１】上記エバネッセント波と表面プラズモン波
の波数が一致する入射角θのとき、エバネッセント波
は、表面プラズモンの励起に使われ、光電子検出器５０
５が検出する反射光の光量が減少する。ＳＰＲ現象は、
プリズム５０４および金属薄膜５０６に接した被測定物
の屈折率に依存するため、例えば、被測定物を水とした
場合、図６に示すように、入射角度と反射強度との関係
を示す曲線が、入射角θがある角度（７０度近辺）で極
小を持つものとして得られる。When the wave number of the evanescent wave coincides with the wave number of the surface plasmon wave, the evanescent wave is used for exciting the surface plasmon, and the photoelectron detector 50
5 decreases the amount of reflected light detected. The SPR phenomenon is
Since it depends on the refractive index of the object to be measured in contact with the prism 504 and the metal thin film 506, for example, when the object to be measured is water, a curve indicating the relationship between the incident angle and the reflection intensity is obtained as shown in FIG. , The incident angle θ has a minimum at a certain angle (around 70 degrees).
【００１２】この特徴は物質固有のものであるので、図
５に示したＳＰＲ現象測定によれば、入射角θを変化さ
せて反射強度が極小となるピークの出現する入射角度θ
を求めることで、例えば、被測定物の温度変化による屈
折率変化を測定することができる。また、金属薄膜５０
６上に抗体などを固定化してセンサ膜とし、被測定対象
の抗原と上記抗体との結合によるセンサ膜の屈折率変化
を、前述したことにより測定することで、抗体（特定物
質）の定量を行うことができる。Since this characteristic is inherent to the substance, according to the SPR phenomenon measurement shown in FIG. 5, the incident angle θ at which the peak at which the reflection intensity is minimized appears by changing the incident angle θ.
, It is possible to measure, for example, a change in the refractive index due to a change in the temperature of the measured object. The metal thin film 50
The antibody (specific substance) can be quantified by immobilizing an antibody or the like on 6 to form a sensor membrane and measuring a change in the refractive index of the sensor membrane due to the binding between the antigen to be measured and the antibody as described above. It can be carried out.
【００１３】[0013]
【発明が解決しようとする課題】ところで、上述したよ
うに、金属薄膜５０６を固定した透明基板５０７は、マ
ッチングオイル５０８で、プリズム５０４に固着させて
いる。図５（ａ）に示すように、プリズム５０４の所定
面に、シリコーンオイルをベースとしたマッチングオイ
ル５０８を滴下し、この上に透明基板５０７を貼り合わ
せることで、金属薄膜５０６を透明基板５０７およびマ
ッチングオイル５０８を介してプリズム５０４に張り付
ける。As described above, the transparent substrate 507 to which the metal thin film 506 is fixed is fixed to the prism 504 with the matching oil 508. As shown in FIG. 5A, a matching oil 508 based on silicone oil is dropped on a predetermined surface of the prism 504, and a transparent substrate 507 is bonded thereon, thereby forming the metal thin film 506 into a transparent substrate 507 and a transparent substrate 507. Affixed to prism 504 via matching oil 508.
【００１４】このようにすることで、金属薄膜５０６は
透明基板５０７と共に容易にプリズム５０４より剥離で
きるので、金属薄膜５０６の交換が容易になる。金属薄
膜５０６が、プリズム５０４に完全に固定されている場
合、一度測定を行った後は、金属薄膜の測定面を洗浄し
てからつぎの測定を行う必要があった。しかしながら、
上述したようにマッチングオイル５０８で張り付けるよ
うにすれば、金属薄膜５０６を測定の後で容易に新しい
ものに交換できるようになり、金属膜の洗浄を行わなく
てよいという利点がある。By doing so, the metal thin film 506 can be easily separated from the prism 504 together with the transparent substrate 507, so that the metal thin film 506 can be easily replaced. In the case where the metal thin film 506 is completely fixed to the prism 504, after the measurement is performed once, it is necessary to clean the measurement surface of the metal thin film and then perform the next measurement. However,
When the metal thin film 506 is applied with the matching oil 508 as described above, the metal thin film 506 can be easily replaced with a new one after the measurement, and there is an advantage that the metal film need not be cleaned.
【００１５】しかしながら、マッチングオイルを用いる
場合、所定の測定間隔でプリズム面の洗浄を行う必要が
発生してしまう。また、金属薄膜の上に直接試料を接触
させて測定する場合、マッチングオイルが試料に混合し
てしまう危険性がある。本発明は、以上のような問題点
を解消するためになされたものであり、金属薄膜を固定
した透明基板とプリズムとを貼り合わせるなど、光学部
品同士を貼り合わせるとき、従来より容易に貼り合わせ
ることができるようにすることを目的とする。However, when the matching oil is used, it becomes necessary to clean the prism surface at predetermined measurement intervals. Further, when the measurement is performed by directly contacting the sample on the metal thin film, there is a risk that the matching oil may be mixed with the sample. The present invention has been made in order to solve the above problems, and when bonding optical components together, such as bonding a transparent substrate and a prism to which a metal thin film is fixed, when bonding optical components to each other, bonding is easier than in the past. The purpose is to be able to.
【００１６】[0016]
【課題を解決するための手段】本発明の屈折率整合透明
フィルムは、２つの透明部材を着脱可能に貼り合わせる
屈折率整合透明フィルムであって、ポリ塩化ビニルと可
塑剤とから構成され、例えば、可塑剤が、ポリ塩化ビニ
ルと可塑剤の重量比が１：５となる状態より少ない状態
で構成され、上記透明部材と実質的に屈折率が同一に形
成されたものである。この発明によれば、屈折率整合透
明フィルムで貼り合わされた一方の透明部材に入射した
光が、屈折率整合透明フィルムのところで屈折すること
なく、他方の透明部材より出射する。SUMMARY OF THE INVENTION The refractive index matching transparent film of the present invention is a refractive index matching transparent film in which two transparent members are detachably attached, and is composed of polyvinyl chloride and a plasticizer. The plasticizer is formed in a state where the weight ratio of polyvinyl chloride to the plasticizer is less than 1: 5, and the refractive index is substantially the same as that of the transparent member. According to the present invention, light incident on one transparent member bonded by the refractive index matching transparent film is emitted from the other transparent member without being refracted at the refractive index matching transparent film.
【００１７】上記発明において、可塑剤は、フタル酸ジ
オクチルと燐酸トリクレジルとから構成されたもの、も
しくは、フタル酸ジオクチルと透明部材と実質的に屈折
率が同一のマッチングオイルとから構成されたものであ
ればよい。また、可塑剤は、マッチングオイルに比較し
てフタル酸ジオクチルの方が多く含まれているようにす
る。In the above invention, the plasticizer is composed of dioctyl phthalate and tricresyl phosphate, or is composed of dioctyl phthalate, a transparent member and a matching oil having substantially the same refractive index as the transparent member. I just need. In addition, the plasticizer is designed to contain more dioctyl phthalate than the matching oil.
【００１８】本発明の屈折率整合透明フィルムを利用し
たセルは、上記屈折率整合透明フィルムが裏面に貼り合
わされた透明基板と、この透明基板表面に形成された金
属薄膜膜と、この金属薄膜周囲に形成された枠体とを備
え、金属薄膜と枠体とにより、金属薄膜を底とする容器
が構成されたものである。A cell using the refractive index matching transparent film of the present invention comprises a transparent substrate having the above-mentioned refractive index matching transparent film bonded to the back surface, a metal thin film formed on the surface of the transparent substrate, and a metal thin film surrounding the metal thin film. And a container having the metal thin film as the bottom is constituted by the metal thin film and the frame.
【００１９】[0019]
【発明の実施の形態】以下、本発明の実施の形態につい
て図を参照して説明する。 ＜実施の形態１＞図１は、本発明の実施の形態における
屈折率整合透明フィルムを用いたＳＰＲ現象測定装置の
構成を示す構成図である。図１（ａ）のＳＰＲ測定装置
は、光源１０１、Ｐ偏光光のみを通過させる偏光板１０
２およびレンズ１０３、プリズム１０４、光電子検出器
１０５、金属薄膜１０６、プリズム１０４と同じ屈折率
のガラスや高分子透明体の透明基板１０７を備えてい
る。Embodiments of the present invention will be described below with reference to the drawings. <Embodiment 1> FIG. 1 is a configuration diagram showing a configuration of an SPR phenomenon measuring apparatus using a refractive index matching transparent film according to an embodiment of the present invention. The SPR measuring apparatus shown in FIG. 1A includes a light source 101 and a polarizing plate 10 that allows only P-polarized light to pass therethrough.
2, a lens 103, a prism 104, a photoelectron detector 105, a metal thin film 106, and a transparent substrate 107 made of glass or a polymer transparent material having the same refractive index as the prism 104.
【００２０】加えて、本実施の形態では、透明基板１０
７とプリズム１０４との貼り合わせに、透明粘着フィル
ム（屈折率整合透明フィルム）１０８を用いるようにし
た。透明粘着フィルム１０８は、プリズム１０４と透明
基板１０７に屈折率が整合している。透明粘着フィルム
１０８は、ポリ塩化ビニルに可塑剤を加えた液を、テト
ラヒドロフラン溶液を用いたキャスト法により、フィル
ム状にしたものである。ポリ塩化ビニルとこれに加える
可塑剤の重量比は、１対５より可塑剤が少なければ、所
望とする薄膜化が可能となる。In addition, in the present embodiment, the transparent substrate 10
A transparent adhesive film (refractive index matching transparent film) 108 was used for bonding the 7 and the prism 104. The refractive index of the transparent adhesive film 108 matches that of the prism 104 and the transparent substrate 107. The transparent adhesive film 108 is a film obtained by casting a liquid obtained by adding a plasticizer to polyvinyl chloride by a casting method using a tetrahydrofuran solution. If the weight ratio of polyvinyl chloride to the plasticizer to be added is less than 1: 5, the desired thin film can be obtained.
【００２１】可塑剤は、フタル酸ジオクチル＋マッチン
グオイル、もしくは、フタル酸ジオクチル＋燐酸トリク
レジルを用いればよい。フタル酸ジオクチル＋マッチン
グオイルの場合、重量比は６対４よりもフタル酸ジオク
チルの量を多くする。また、フタル酸ジオクチル＋燐酸
トリクレジルの場合、透明粘着フィルム１０８の屈折率
を、プリズム１０４に整合させるために、各々の重量比
を任意に変える。フタル酸ジオクチルの屈折率は１．４
８４２であり、燐酸トリクレジルの屈折率は１．５５４
９である。例えば、規格ＢＫ７のガラス剤からなるプリ
ズム１０４の場合、この屈折率は１．５１６であるた
め、屈折率を合わせるためには、フタル酸ジオクチルと
燐酸トリクレジルとの重量比を１対１とすればよい。As the plasticizer, dioctyl phthalate + matching oil or dioctyl phthalate + tricresyl phosphate may be used. In the case of dioctyl phthalate + matching oil, the weight ratio makes the amount of dioctyl phthalate larger than 6: 4. In the case of dioctyl phthalate + tricresyl phosphate, the weight ratio of the transparent adhesive film 108 is arbitrarily changed in order to match the refractive index with the prism 104. Dioctyl phthalate has a refractive index of 1.4.
842, and the refractive index of tricresyl phosphate is 1.554.
9 For example, in the case of the prism 104 made of a glass material of the standard BK7, the refractive index is 1.516. To match the refractive indices, the weight ratio of dioctyl phthalate to tricresyl phosphate is set to 1: 1. Good.
【００２２】以上に示した材料、例えば、ポリ塩化ビニ
ル０．２ｇと、可塑剤としてフタル酸ジオクチル０．６
ｇ，マッチングオイル０．４ｇを混合して原料溶液を作
製する。この原料溶液をテトラヒドロフラン溶液上に滴
下し、テトラヒドロフラン溶液の液面に原料溶液を展開
させてフィルム状とすることで、透明粘着フィルム１０
８が作製できる。The materials shown above, for example, 0.2 g of polyvinyl chloride, and 0.6 g of dioctyl phthalate as a plasticizer
g and 0.4 g of matching oil to prepare a raw material solution. This raw material solution is dropped on the tetrahydrofuran solution, and the raw material solution is spread on the liquid surface of the tetrahydrofuran solution to form a film, whereby the transparent adhesive film 10 is formed.
8 can be produced.
【００２３】このようにして作製した透明粘着フィルム
１０８は、図１（ｂ）に示すように、金属薄膜１０６が
形成された透明基板１０７とプリズム１０４の平坦面と
の間に配置して用いる。透明粘着フィルム１０８は、粘
着性があるため、透明基板１０７をプリズム１０４の平
坦面に接着して保持することができる。また、透明粘着
フィルム１０８は、透明基板１０７とプリズム１０４と
を完全に接着固定するものではなく、容易に剥がすこと
が可能である。As shown in FIG. 1B, the transparent pressure-sensitive adhesive film 108 produced in this manner is disposed between the transparent substrate 107 on which the metal thin film 106 is formed and the flat surface of the prism 104 for use. Since the transparent adhesive film 108 has adhesiveness, the transparent substrate 107 can be adhered and held on the flat surface of the prism 104. Further, the transparent adhesive film 108 does not completely adhere and fix the transparent substrate 107 and the prism 104, but can be easily peeled off.
【００２４】例えば、まず、規格ＢＫ７のガラス材より
透明基板１０７を形成し、蒸着法やスパッタ法などによ
り透明基板１０７の主面全域に金属薄膜１０６を形成す
る。金属薄膜１０６は、酸化などの化学反応が起きにく
い材料が好ましく、例えば金を用いるようにすればよ
い。また、金属薄膜１０６形成面に枠体２１１を形成
し、図２に示すように、透明基板１０７，金属薄膜１０
６および枠体２１１で容器が形成された状態とする。For example, first, a transparent substrate 107 is formed from a glass material of standard BK7, and a metal thin film 106 is formed over the entire main surface of the transparent substrate 107 by an evaporation method, a sputtering method, or the like. The metal thin film 106 is preferably made of a material that does not easily cause a chemical reaction such as oxidation. For example, gold may be used. Further, a frame 211 is formed on the surface on which the metal thin film 106 is formed, and as shown in FIG.
6 and the frame 211 are in a state where a container is formed.
【００２５】次いで、透明基板１０７の裏面に、透明基
板１０７およびプリズム１０４と同一屈折率に作製した
透明粘着フィルム１０８を張り付け、被測定溶液を収容
する容器が形成されたセル２０１とする。ＳＰＲ現象の
測定時には、このセル２０１の透明粘着フィルム１０８
の面をプリズム１０４の平坦面に張り付け、枠体２１１
による容器内に被測定液を収容すればよい。Next, a transparent adhesive film 108 having the same refractive index as that of the transparent substrate 107 and the prism 104 is adhered to the back surface of the transparent substrate 107 to form a cell 201 in which a container for storing the solution to be measured is formed. When measuring the SPR phenomenon, the transparent adhesive film 108
Is attached to the flat surface of the prism 104, and the frame body 211
The liquid to be measured may be accommodated in a container according to the above.
【００２６】つぎに、実際の測定について説明する。ま
ず、金属薄膜１０６表面に、Self-Assembly膜作製法に
よって、ヒトアルブミンを固定化することで、金属薄膜
１０６表面にヒトアルブミンからなるセンサ膜を形成す
る。次いで、測定対象である抗ヒトアルブミンの水溶液
をセル２０１内に収容し、プリズム１０４を介して金属
薄膜１０６の裏面に入射する光の角度を変化させ、反射
強度が極小となるピークの出現する入射角度θを求める
ことで、センサ膜の屈折率変化を測定した。Next, the actual measurement will be described. First, human albumin is immobilized on the surface of the metal thin film 106 by a Self-Assembly film forming method, so that a sensor film made of human albumin is formed on the surface of the metal thin film 106. Next, an aqueous solution of anti-human albumin to be measured is accommodated in the cell 201, the angle of light incident on the back surface of the metal thin film 106 via the prism 104 is changed, and the incidence at which a peak at which the reflection intensity becomes minimal appears. The change in the refractive index of the sensor film was measured by obtaining the angle θ.
【００２７】この屈折率変化の測定の結果、図３に示す
ように、測定対象の抗ヒトアルブミンの濃度に比例し
て、上記ピークの出現する角度のシフト量が変化した。
この応答は、従来のように透明基板とプリズムをマッチ
ングオイルで貼り合わせた場合と、同様であった。図３
では、白四角で上記透明粘着フィルム１０８を用いた場
合を示し、白丸でマッチングオイルを用いた場合を示し
ており、両者の間にはほとんど差がない。As a result of the measurement of the change in the refractive index, as shown in FIG. 3, the shift amount of the angle at which the peak appears changes in proportion to the concentration of the anti-human albumin to be measured.
This response was similar to the case where the transparent substrate and the prism were bonded with matching oil as in the related art. FIG.
In the figure, a white square shows a case where the transparent adhesive film 108 is used, and a white circle shows a case where matching oil is used, and there is almost no difference between the two.
【００２８】＜実施の形態２＞つぎに、本発明の他の実
施の形態について説明する。上述では、ポリ塩化ビニル
に可塑剤としてフタル酸ジオクチルとマッチングオイル
とを加えるようにしたが、これに限るものではない。可
塑剤として、フタル酸ジオクチルと燐酸トリクレジルと
を加えるようにしてもよい。Second Embodiment Next, another embodiment of the present invention will be described. In the above description, dioctyl phthalate and a matching oil are added as a plasticizer to polyvinyl chloride, but the present invention is not limited to this. Dioctyl phthalate and tricresyl phosphate may be added as plasticizers.
【００２９】例えば、ポリ塩化ビニル０．２ｇと、可塑
剤としてフタル酸ジオクチル０．５ｇ，燐酸トリクレジ
ル０．５ｇを混合して原料溶液を作製する。この原料溶
液をテトラヒドロフラン溶液上に滴下し、テトラヒドロ
フラン溶液の液面に原料溶液を展開させてフィルム状と
することで、透明粘着フィルム１０８を作製するように
してもよい。For example, a raw material solution is prepared by mixing 0.2 g of polyvinyl chloride, 0.5 g of dioctyl phthalate and 0.5 g of tricresyl phosphate as plasticizers. The transparent adhesive film 108 may be produced by dropping this raw material solution onto a tetrahydrofuran solution and developing the raw material solution on the liquid surface of the tetrahydrofuran solution to form a film.
【００３０】この透明粘着フィルム１０８は、屈折率が
１．５１６１であり、規格ＢＫ７ガラスの屈折率と一致
する。このようにして作製した透明粘着フィルム１０８
は、前述した実施の形態と同様に、金属薄膜１０６が形
成された透明基板１０７とプリズム１０４の平坦面との
間に配置して用いる（図１（ｂ））。This transparent adhesive film 108 has a refractive index of 1.5161, which matches the refractive index of standard BK7 glass. The transparent adhesive film 108 thus produced
Is used between the transparent substrate 107 on which the metal thin film 106 is formed and the flat surface of the prism 104, as in the above-described embodiment (FIG. 1B).
【００３１】つぎに、実際の測定について説明する。ま
ず、金属薄膜１０６表面に、Self-Assembly膜作製法に
よって、ヒトアルブミンを固定化することで、金属薄膜
１０６表面にヒトアルブミンからなるセンサ膜を形成す
る。次いで、測定対象である抗ヒトアルブミンの水溶液
をセル２０１内に収容し、プリズム１０４を介して金属
薄膜１０６の裏面に入射する光の角度を変化させ、反射
強度が極小となるピークの出現する入射角度θを求める
ことで、センサ膜の屈折率変化を測定した。Next, the actual measurement will be described. First, human albumin is immobilized on the surface of the metal thin film 106 by a Self-Assembly film forming method, so that a sensor film made of human albumin is formed on the surface of the metal thin film 106. Next, an aqueous solution of anti-human albumin to be measured is accommodated in the cell 201, the angle of light incident on the back surface of the metal thin film 106 via the prism 104 is changed, and the incidence at which a peak at which the reflection intensity becomes minimal appears. The change in the refractive index of the sensor film was measured by obtaining the angle θ.
【００３２】この屈折率変化の測定の結果、図４に示す
ように、測定対象の抗ヒトアルブミンの濃度に比例し
て、上記ピークの出現する角度のシフト量が変化した。
この応答は、従来のように透明基板とプリズムをマッチ
ングオイルで貼り合わせた場合と、同様であった。図４
では、白四角で上記透明粘着フィルム１０８を用いた場
合を示し、白丸でマッチングオイルを用いた場合を示し
ており、どちらも濃度とシフト量との間に良好な直線性
が得られた。As a result of the measurement of the change in the refractive index, as shown in FIG. 4, the amount of shift of the angle at which the peak appears changed in proportion to the concentration of the anti-human albumin to be measured.
This response was similar to the case where the transparent substrate and the prism were bonded with matching oil as in the related art. FIG.
In the graph, white squares indicate the case where the transparent adhesive film 108 was used, and white circles indicate the case where matching oil was used. In each case, good linearity was obtained between the density and the shift amount.
【００３３】[0033]
【発明の効果】以上説明したように、本発明によれば、
屈折率整合透明フィルムを用いて貼り合わせるようにし
たので、金属薄膜を固定した透明基板とプリズムとを貼
り合わせるなど、光学部品同士を貼り合わせるとき、従
来より容易に貼り合わせることができるようになるとい
う優れた効果が得られる。As described above, according to the present invention,
Since it is bonded using a refractive index matching transparent film, when bonding optical components to each other, such as bonding a transparent substrate on which a metal thin film is fixed to a prism, it becomes easier to bond than before. An excellent effect is obtained.
【図１】 本発明の実施の形態における屈折率整合透明
フィルムを用いたＳＰＲ現象測定装置の構成を示す断面
図である。FIG. 1 is a cross-sectional view illustrating a configuration of an SPR phenomenon measuring apparatus using a refractive index matching transparent film according to an embodiment of the present invention.
【図２】 本発明の実施の形態における屈折率整合透明
フィルムを用いたＳＰＲ現象測定装置の他の形態におけ
る部分的な構成を示す断面図である。FIG. 2 is a cross-sectional view showing a partial configuration of another embodiment of the SPR phenomenon measuring device using the refractive index matching transparent film according to the embodiment of the present invention.
【図３】 抗ヒトアルブミンを被測定対象としたとき
の、濃度と反射強度が極小となるピークの出現する入射
角度θのシフト量との関係を示す相関図である。FIG. 3 is a correlation diagram showing the relationship between the concentration and the shift amount of the incident angle θ at which a peak at which the reflection intensity is minimal occurs when anti-human albumin is the measurement target.
【図４】 抗ヒトアルブミンを被測定対象としたとき
の、濃度と反射強度が極小となるピークの出現する入射
角度θのシフト量との関係を示す相関図である。FIG. 4 is a correlation diagram showing the relationship between the concentration and the shift amount of the incident angle θ at which a peak at which the reflection intensity is minimal occurs when anti-human albumin is a measurement target.
【図５】 従来のＳＰＲ現象測定装置の構成を示す断面
図である。FIG. 5 is a cross-sectional view showing a configuration of a conventional SPR phenomenon measuring device.
【図６】 ＳＰＲ現象測定装置を用いた測定結果を示す
説明図である。FIG. 6 is an explanatory diagram showing a measurement result using an SPR phenomenon measurement device.
１０１…光源、１０２…偏光板、１０３…レンズ、１０
４…プリズム、１０５…光電子検出器、１０６…金属薄
膜、１０７…透明基板、１０８…透明粘着フィルム、２
０１…セル、２１１…枠体。101: light source, 102: polarizing plate, 103: lens, 10
4 prism, 105 photoelectron detector, 106 thin metal film, 107 transparent substrate, 108 transparent adhesive film, 2
01 ... cell, 211 ... frame.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ Ｃ０８Ｌ 27/06 Ｃ０８Ｌ 27/06 91/00 91/00 Ｇ０１Ｎ 21/01 Ｇ０１Ｎ 21/01 Ｂ 21/03 21/03 Ｚ 21/41 21/41 Ｚ (72)発明者 浅野 泰一 青森県八戸市大字田面木字上野平16番地 １高専宿舎202 (72)発明者 正留 隆 福岡県大牟田市東萩尾町150 (72)発明者 今任 稔彦 福岡県福岡市早良区南庄二丁目８番８号 307 (72)発明者 伏貫 義十 鹿児島県鹿児島市武岡一丁目３番地16 (72)発明者 田部井 久男 東京都新宿区西新宿二丁目１番１号 エ ヌ・ティ・ティ・アドバンステクノロジ 株式会社内 (72)発明者 飛田 達也 東京都新宿区西新宿二丁目１番１号 エ ヌ・ティ・ティ・アドバンステクノロジ 株式会社内 (56)参考文献 特開 平10−38800（ＪＰ，Ａ) 特開 平10−104163（ＪＰ，Ａ) 特表 平５−505874（ＪＰ，Ａ) 特表 平５−504626（ＪＰ，Ａ) 特表 平６−506298（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 21/00 - 21/01 G01N 21/17 - 21/61 G01N 21/62 - 21/74 G01N 21/03 - 21/15 ＪＩＣＳＴファイル（ＪＯＩＳ) 実用ファイル（ＰＡＴＯＬＩＳ) 特許ファイル（ＰＡＴＯＬＩＳ)────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. 7 Identification code FI C08L 27/06 C08L 27/06 91/00 91/00 G01N 21/01 G01N 21/01 B 21/03 21/03 Z 21 / 41 21/41 Z (72) Inventor Taiichi Asano 16 Uenodaira, Hachinohe-shi, Aomori Prefecture, Uenodaira, 16-fig. Toshihiko 307-8-2-8 Minamisho, Sawara-ku, Fukuoka City, Japan Inventor Yoshito Fuzuki 1-3-16 Takeoka, Kagoshima-shi, Kagoshima Prefecture, Japan No. 1 NTT Advanced Technology Co., Ltd. (72) Inventor Tatsuya Tobita 2-1-1 Nishi Shinjuku, Shinjuku-ku, Tokyo NTT Advanced Technology Co., Ltd. (56) Reference Literature JP-A-10-38800 (JP, A) JP-A-10-104163 (JP, A) JP-T 5-505874 (JP, A) JP-T 5-504626 (JP, A) JP-T 6-506298 (JP, A) (58) Fields investigated (Int. Cl. 7 , DB name) G01N 21/00-21/01 G01N 21/17-21/61 G01N 21/62-21/74 G01N 21/03- 21/15 JICST file (JOIS) Practical file (PATOLIS) Patent file (PATOLIS)
Claims (6)
る屈折率整合透明フィルムであって、 ポリ塩化ビニルと可塑剤とから構成され、前記透明部材
と実質的に屈折率が同一に形成されたことを特徴とする
屈折率整合透明フィルム。1. A refractive index matching transparent film for removably attaching two transparent members, comprising a polyvinyl chloride and a plasticizer, wherein the refractive index of the transparent member is substantially the same as that of the transparent member. A refractive index matching transparent film characterized by the above-mentioned.
において、 前記可塑剤は、前記ポリ塩化ビニルと前記可塑剤の重量
比が１：５となる状態より少ないことを特徴とする屈折
率整合透明フィルム。2. The refractive index matching transparent film according to claim 1, wherein the plasticizer is less than a weight ratio of the polyvinyl chloride to the plasticizer of 1: 5. Transparent film.
フィルムにおいて、 前記可塑剤は、フタル酸ジオクチルと燐酸トリクレジル
とから構成されたものであることを特徴とする屈折率整
合透明フィルム。3. The refractive index matching transparent film according to claim 1, wherein the plasticizer is composed of dioctyl phthalate and tricresyl phosphate.
フィルムにおいて、 前記可塑剤は、フタル酸ジオクチルと前記透明部材と実
質的に屈折率が同一のマッチングオイルとから構成され
たものであることを特徴とする屈折率整合透明フィル
ム。4. The refractive index matching transparent film according to claim 1, wherein the plasticizer is composed of dioctyl phthalate and a matching oil having substantially the same refractive index as the transparent member. A refractive index matching transparent film characterized by the above-mentioned.
において、 前記可塑剤は、前記マッチングオイルに比較してフタル
酸ジオクチルの方が多く含まれていることを特徴とする
屈折率整合透明フィルム。5. The refractive index matching transparent film according to claim 4, wherein the plasticizer contains more dioctyl phthalate than the matching oil. .
率整合透明フィルムが裏面に貼り合わされた透明基板
と、 この透明基板表面に形成された金属薄膜と、 この金属薄膜周囲に形成された枠体とを備え、 前記金属薄膜と前記枠体とにより、前記金属薄膜を底と
する容器が構成されたことを特徴とする屈折率整合透明
フィルムを利用したセル。6. A transparent substrate having the refractive index matching transparent film according to any one of claims 1 to 5 bonded to a back surface, a metal thin film formed on the surface of the transparent substrate, and a metal thin film formed around the metal thin film. A cell using a refractive index matching transparent film, wherein a container having the metal thin film as a bottom is constituted by the metal thin film and the frame.
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| JP2001015558A JP3356212B2 (en) | 2001-01-24 | 2001-01-24 | Index matching transparent film and cell using index matching transparent film |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006003214A (en) * | 2004-06-17 | 2006-01-05 | Muroran Institute Of Technology | Surface plasmon resonance phenomenon measuring instrument |
| JP2006112808A (en) * | 2004-10-12 | 2006-04-27 | Fujikura Ltd | Surface plasmon sensor |
| JP2008513772A (en) * | 2004-09-15 | 2008-05-01 | エイジェンシー フォー サイエンス， テクノロジー アンド リサーチ | Surface plasmon resonance and quartz crystal microbalance sensor |
| WO2010050203A1 (en) | 2008-10-30 | 2010-05-06 | 日本電信電話株式会社 | Measuring chip installation/removal device, spr measurement system, and measuring chip installation/removal method |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4076962B2 (en) * | 2003-04-23 | 2008-04-16 | 独立行政法人科学技術振興機構 | Differential surface plasmon resonance phenomenon measuring apparatus and measuring method thereof |
| KR101043444B1 (en) * | 2009-03-31 | 2011-06-22 | 케이맥(주) | Optic Interface for Surface Plasmon Resonance Sensor |
-
2001
- 2001-01-24 JP JP2001015558A patent/JP3356212B2/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006003214A (en) * | 2004-06-17 | 2006-01-05 | Muroran Institute Of Technology | Surface plasmon resonance phenomenon measuring instrument |
| US7545500B2 (en) | 2004-06-17 | 2009-06-09 | Muroran Institute Of Technology | Surface plasmon resonance phenomenon measuring equipment |
| JP2008513772A (en) * | 2004-09-15 | 2008-05-01 | エイジェンシー フォー サイエンス， テクノロジー アンド リサーチ | Surface plasmon resonance and quartz crystal microbalance sensor |
| JP2006112808A (en) * | 2004-10-12 | 2006-04-27 | Fujikura Ltd | Surface plasmon sensor |
| WO2010050203A1 (en) | 2008-10-30 | 2010-05-06 | 日本電信電話株式会社 | Measuring chip installation/removal device, spr measurement system, and measuring chip installation/removal method |
| US8531658B2 (en) | 2008-10-30 | 2013-09-10 | Nippon Telegraph And Telephone Corporation | Measuring chip device using magnets for installation/removal |
Also Published As
| Publication number | Publication date |
|---|---|
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