JP3085967B2 - Automatic blood collection method - Google Patents
Automatic blood collection methodInfo
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- JP3085967B2 JP3085967B2 JP22866690A JP22866690A JP3085967B2 JP 3085967 B2 JP3085967 B2 JP 3085967B2 JP 22866690 A JP22866690 A JP 22866690A JP 22866690 A JP22866690 A JP 22866690A JP 3085967 B2 JP3085967 B2 JP 3085967B2
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- sample
- blood
- sample collection
- liquid level
- electrode
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Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、血液自動採取方法に関する。Description: TECHNICAL FIELD The present invention relates to an automatic blood sampling method.
従来、血液試料の自動分析において、試料採取ノズル
を試料容器内に自動的に下降させ、血液試料中に浸漬し
た後、所定量の血液試料を吸引採取していた。その際、
試料採取ノズルが試料液面から所定の深さ位置に達した
ときに試料採取ノズルの下降を停止させるように、液面
検知が行なわれていた。例えば、実開昭54−19790号公
報に開示されている如く、試料採取ノズル及びこれと一
体に並設された電極棒を支持した支持具を試料液面に向
けて下降させ、これら試料採取ノズルと電極棒との間に
血液試料が侵入して電気伝導が生じたことを計測するこ
とによって、試料採取ノズルと電極棒とが血液試料の液
面に到達したことを検知していた。Conventionally, in an automatic analysis of a blood sample, a predetermined amount of a blood sample has been suctioned and collected after automatically lowering a sample collection nozzle into a sample container and immersing the sample in the blood sample. that time,
Liquid level detection has been performed so that the lowering of the sample collection nozzle is stopped when the sample collection nozzle reaches a predetermined depth position from the sample liquid level. For example, as disclosed in Japanese Utility Model Application Laid-Open No. 54-19790, a support for supporting a sample collecting nozzle and an electrode rod integrally provided therewith is lowered toward a sample liquid level, and these sample collecting nozzles are lowered. By measuring that the blood sample has penetrated into the gap between the electrode and the electrode rod and the electric conduction has occurred, it has been detected that the sample collection nozzle and the electrode rod have reached the liquid level of the blood sample.
しかしながら、血液試料を採取した後に、血液試料中
の血清フィブリン等の血液凝固成分が、試料採取ノズル
と電極棒との間に付着しやすい。これにより、試料採取
ノズルと電極棒との間に電気が導通した状態のままにな
る。このため、その後の試料採取サイクルにおいて、試
料採取ノズルが試料液面に到達しなくとも、液面検知状
態と同様な現象が生じる。この結果、支持具の下降が停
止し、適切な試料採取を行うことができない問題があっ
た。However, after collecting a blood sample, blood coagulation components such as serum fibrin in the blood sample tend to adhere between the sampling nozzle and the electrode rod. As a result, the state where electricity is conducted between the sampling nozzle and the electrode rod remains. For this reason, in the subsequent sampling cycle, a phenomenon similar to the liquid level detection state occurs even if the sampling nozzle does not reach the sample liquid level. As a result, there is a problem that the lowering of the support stops, and it is not possible to perform appropriate sampling.
本発明は、かかる点に鑑みてなされたものであり、液
面検出の誤動作を生じることなく、所定量の血液試料の
採取を適切に行うことができる血液自動採取方法を提供
することを目的とする。The present invention has been made in view of the above point, and an object of the present invention is to provide an automatic blood collection method capable of appropriately collecting a predetermined amount of a blood sample without causing a malfunction in liquid level detection. I do.
請求項１に係る本発明は、試料採取ノズルと電極部の
両部材をお互いに離間し、かつ並列して支持する液面検
知センサを、試料容器に向かって下降させる工程と、前
記試料用ノズルと前記電極部の間への血液検体の侵入に
よる両部材間の電気伝導の変化により、前記試料採取ノ
ズルの先端部が前記血液試料の液面に到達したことを検
知して前記試料採取ノズルの先端部を該血液試料の所定
の深さで停止させる工程と、試料採取作業終了後、次の
血液検体の採取を開始する前に、前記試料採取ノズル及
び電極間の電気伝導の有無を測定し、両部材間に付着し
た前記血液凝固成分の付着を検出する工程を具備するこ
とを特徴とする血液自動採取方法である。The present invention according to claim 1, wherein a step of lowering a liquid level detection sensor that supports both a sample collection nozzle and an electrode unit apart from each other and supports the sample collection nozzle toward a sample container, and the sample nozzle And a change in electrical conduction between the two members due to the invasion of the blood sample between the electrode portions, by detecting that the tip of the sample collection nozzle has reached the liquid level of the blood sample, and The step of stopping the tip at a predetermined depth of the blood sample, and after the end of the sample collection operation, before starting the next blood sample collection, measuring the presence or absence of electrical conduction between the sample collection nozzle and the electrode. And a step of detecting the adhesion of the blood coagulation component adhering between the two members.
また、請求項２に係る発明は、互いに離間した１対の
電極部と試料採取ノズルとを並設して支持する液面検知
センサを、試料容器に収容された血液試料に向かって下
降させる工程と、前記電極部の間への血液検体の侵入に
よる両部材間の電気伝導の変化により、前記試料採取ノ
ズルの先端部が前記血液試料の液面に到達したことを検
知して前記試料採取ノズルの先端部を該血液試料の所定
の深さで停止させる工程と、試料採取作業了後、次の血
液検体の採取を開始する前に、前記試料採取ノズル及び
電極間の電気伝導の有無を測定し、両部材間に付着した
前記血液凝固成分の付着を検出する工程を具備すること
を特徴とする血液自動採取方法である。Further, the invention according to claim 2 is a step of lowering a liquid level detection sensor that supports a pair of electrode portions and a sample collection nozzle that are spaced apart from each other in parallel toward a blood sample contained in a sample container. And a change in electrical conduction between the two members due to invasion of the blood sample between the electrode portions, by detecting that the tip of the sample collection nozzle has reached the liquid level of the blood sample, and detecting the sample collection nozzle. Stopping the tip of the blood sample at a predetermined depth and measuring the presence or absence of electrical conduction between the sample collection nozzle and the electrode after the sample collection operation and before starting the next blood sample collection. And a step of detecting the adhesion of the blood coagulation component adhering between the two members.
本発明の血液自動採取方法によれば、一つの試料採取
が終了した後、次の試料採取を行う前に、液面検知セン
サが血液検体と接触していない状態で、試料採取ノズル
と電極部との間の電気伝導を測定する。この電気伝導の
測定値に基づいて、試料採取ノズルと電極部との間の血
液凝固成分の付着を検出できる。According to the automatic blood sampling method of the present invention, after one sample has been collected and before the next sample is collected, the liquid level detection sensor is not in contact with the blood sample, and the sample collection nozzle and the electrode section are not in contact. Measure the electrical conduction between. Based on the measured value of the electric conduction, the adhesion of the blood coagulation component between the sampling nozzle and the electrode unit can be detected.
以下、本発明の実施例について、図面を参照して詳細
に説明する。Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
第１図は、この実施例で使用した血液自動採取装置の
構成を示す説明図である。FIG. 1 is an explanatory diagram showing a configuration of an automatic blood sampling apparatus used in this embodiment.
まず、試料採取ノズル２と電極３を長手方向に並設さ
せ、かつ所定の間隔をおいて支持する液面検知センサ４
を、駆動装置（図示せず）によって血液試料19を収容す
る試料容器18の液面に向かって下降させる。試料採取ノ
ズル２は、導電性材料からなり、電極を兼ねている。試
料採取ノズル２は、交流電圧源23に接続されている。一
方、電極３は、信号増巾器８に接続されている。First, a liquid level detection sensor 4 supporting a sample collection nozzle 2 and an electrode 3 in a longitudinal direction and supporting them at a predetermined interval.
Is lowered toward the liquid surface of the sample container 18 containing the blood sample 19 by a driving device (not shown). The sampling nozzle 2 is made of a conductive material and also serves as an electrode. The sampling nozzle 2 is connected to an AC voltage source 23. On the other hand, the electrode 3 is connected to a signal amplifier 8.
この際に、液面検知センサ４が液面に接触していない
状態では、信号増巾器８の入力信号が、電極４と信号増
巾器８の間に接続された抵抗７を通って、0vとなる。こ
れによって、信号増巾器８の出力信号も0vとなり、信号
比較器９に伝達される。0vの出力信号が信号比較器９に
入力されている状態では、信号比較器９の出力信号は、
“非検知状態”を示す非検知信号として、第１の信号変
換器（I/O）10、分析機11、データプロセッサ13により
順次処理される。At this time, when the liquid level detection sensor 4 is not in contact with the liquid level, the input signal of the signal amplifier 8 passes through the resistor 7 connected between the electrode 4 and the signal amplifier 8, and It becomes 0v. As a result, the output signal of the signal amplifier 8 also becomes 0v and is transmitted to the signal comparator 9. In a state where the output signal of 0v is input to the signal comparator 9, the output signal of the signal comparator 9 is
The first signal converter (I / O) 10, the analyzer 11, and the data processor 13 sequentially process the non-detection signal indicating the “non-detection state”.
降下する試料採取ノズル２及び電極３の先端部が血液
試料19の液面に到達すると、血液試料19を介して、試料
採取ノズル２、電極３の間が導通状態となる。これによ
り、交流電圧源23からの交流出力が、試料採取ノズル
２、血液試料19、電極３を介して、信号増巾器８に入力
される。信号増巾器８からの出力信号は、信号比較器９
で比較されて、しきい値を超えた“検知状態”を示す検
知信号となる。検知信号は、信号変換器10を介して分析
機11に伝達される。分析機11は、検知信号に応じて、駆
動装置、試料採取用ポンプ（両方とも図示せず）に制御
信号を出力する。この制御信号によって、実開昭54−19
790号公報に開示されているように、下降する液面検知
センサ４を所定位置で停止させ、試料採取ノズル２に連
結された試料採取用ポンプによって、試料採取ノズル２
を介して所定量の血液試料19を採取する。血液試料19を
採取した後、液面検知センサ４を、試料容器18から最初
の位置まで上昇させる。次いで、液面検知センサ４を反
応容器（図示せず）の方向に水平移動させ、反応容器の
上方で停止させる。さらに、液面検知センサ４を一定位
置まで下降させ、試料採取ノズル２中に吸入された血液
試料19を反応容器へ吐出させる。吐出終了後に、液面検
知センサ４を元の位置に戻す。When the tips of the descending sampling nozzle 2 and the electrode 3 reach the liquid surface of the blood sample 19, the connection between the sampling nozzle 2 and the electrode 3 is established via the blood sample 19. Thus, the AC output from the AC voltage source 23 is input to the signal amplifier 8 via the sampling nozzle 2, the blood sample 19, and the electrode 3. An output signal from the signal amplifier 8 is output to a signal comparator 9.
And a detection signal indicating a “detection state” exceeding the threshold value. The detection signal is transmitted to the analyzer 11 via the signal converter 10. The analyzer 11 outputs a control signal to a driving device and a sampling pump (both not shown) according to the detection signal. By this control signal,
As disclosed in Japanese Patent Publication No. 790, the descending liquid level detection sensor 4 is stopped at a predetermined position, and a sampling pump connected to the sampling nozzle 2 is used for sampling.
A predetermined amount of blood sample 19 is collected via. After collecting the blood sample 19, the liquid level detection sensor 4 is raised from the sample container 18 to the initial position. Next, the liquid level detection sensor 4 is moved horizontally in the direction of the reaction vessel (not shown) and stopped above the reaction vessel. Further, the liquid level detection sensor 4 is lowered to a certain position, and the blood sample 19 sucked into the sample collection nozzle 2 is discharged to the reaction container. After the discharge is completed, the liquid level detection sensor 4 is returned to the original position.
このような血液自動採取装置１による血液試料19の自
動採取操作において、試料採取ノズル２と電極３との間
での血液試料19中の血液凝固成分20の付着を、次のよう
にして検出する。In such an automatic blood sampling operation by the automatic blood sampling apparatus 1, the adhesion of the blood coagulation component 20 in the blood sample 19 between the sample sampling nozzle 2 and the electrode 3 is detected as follows. .
まず、試料採取後、液面検知センサ４を上昇させる。
そして、上昇後所定のタイミングで、次の試料容器18中
の血液試料19の液面を検知する前に、信号比較器９から
の出力信号を、データプロセッサ13で処理する。この際
に、この出力信号が“検知状態”である場合に、試料採
取ノズル２と電極３との間に血液凝固成分20が付着して
いることが検知される。これに対して、データプロセッ
サ13は、第２の信号変換器15を介して接続された表示装
置16、ブザー17に制御信号を伝達する。この制御信号に
応じて、表示装置（CRT）16は、異常として表示すると
共に、ブザー17を鳴らして警報を発する。First, after sampling, the liquid level detection sensor 4 is raised.
At a predetermined timing after the rise, the output signal from the signal comparator 9 is processed by the data processor 13 before the liquid level of the blood sample 19 in the next sample container 18 is detected. At this time, when the output signal is in the “detection state”, it is detected that the blood coagulation component 20 has adhered between the sampling nozzle 2 and the electrode 3. On the other hand, the data processor 13 transmits a control signal to the display device 16 and the buzzer 17 connected via the second signal converter 15. In response to this control signal, the display device (CRT) 16 displays an error and sounds a buzzer 17 to issue an alarm.
次に、血液凝固成分20の付着が検知され、警報が出さ
れた後、液面検知センサ４を、第２図に示すような洗浄
ステーション30に移動して、洗浄容器34に収容する。次
いで、エアポンプ31を起動し、エアポンプ31と電磁弁32
の間を連結する配管33内の気圧を高める。次に、電磁弁
32を開放して、配管33内の激しく圧縮されたエアを、洗
浄容器34内に噴出し、血液凝固成分20を吹き飛ばして、
血液凝固成分20を除去する。Next, after the adhesion of the blood coagulation component 20 is detected and an alarm is issued, the liquid level detection sensor 4 is moved to the washing station 30 as shown in FIG. Next, the air pump 31 is started, and the air pump 31 and the solenoid valve 32 are started.
The pressure inside the pipe 33 connecting between them is increased. Next, the solenoid valve
32 is opened, the strongly compressed air in the pipe 33 is jetted into the washing container 34, and the blood coagulation component 20 is blown off.
The blood coagulation component 20 is removed.
また、血液凝固成分20を、第３図に示す如く、水で洗
浄することもできる。すなわち、洗浄ステーション40に
おいて、洗浄水タンク41内の洗浄水（真水あるいは洗浄
溶液）42をポンプ43によって、洗浄容器44内の血液凝固
成分20に激しく浴びせかけることによって、血液凝固成
分20を洗い流してもよい。In addition, the blood coagulation component 20 can be washed with water as shown in FIG. That is, in the washing station 40, even if the blood coagulation component 20 in the washing water tank 41 is flushed with the pump 43, and the blood coagulation component 20 in the washing container 44 is vigorously poured by the pump 43. Good.
なお、第１図中６は、電極３と信号増幅器８を電気的
に接続する導線、12は、分析機11を制御するためのプロ
グラム、14は、データプロセッサ13を制御するためのプ
ログラム、21、22は、信号比較器９を直流電源（図示せ
ず）に夫々接続するための接続端子である。In FIG. 1, 6 is a conductor for electrically connecting the electrode 3 and the signal amplifier 8, 12 is a program for controlling the analyzer 11, 14 is a program for controlling the data processor 13, 21 , 22 are connection terminals for connecting the signal comparator 9 to a DC power supply (not shown), respectively.
このような血液自動採取方法によれば、試料採取ノズ
ル２と電極３との間に血液凝固成分20が付着しているこ
とを、試料採取ノズル２と電極３との間の電気伝導の測
定値に基づいて検知する。そして、血液凝固成分20の付
着が検出された場合に、血液凝固成分20を空気または水
により除去する。これによって、付着した血液凝固成分
20による血液試料19の液面検知の誤動作を略完全に防ぐ
ことができた。この結果、適切な血液試料19の採取を容
易に達成することができた。According to such an automatic blood sampling method, the fact that the blood coagulation component 20 has adhered between the sample sampling nozzle 2 and the electrode 3 is a measure of the electrical conduction between the sample sampling nozzle 2 and the electrode 3. Detect based on Then, when the attachment of the blood coagulation component 20 is detected, the blood coagulation component 20 is removed by air or water. As a result, attached blood coagulation components
The malfunction of the liquid level detection of the blood sample 19 due to 20 could be almost completely prevented. As a result, appropriate blood sample 19 could be easily collected.
また、血液凝固成分20の付着を警報することによっ
て、どの時に問題が発生したかが判明するため、血液凝
固成分20の付着の対処が容易にできる。また、血液凝固
成分20の除去により、それ以降の液面検知が正しく行わ
れ、試料、試薬等の浪費を防ぐことができる。In addition, the alarm of the adhesion of the blood coagulation component 20 makes it possible to determine at which time the problem has occurred. Further, by removing the blood coagulation component 20, the subsequent liquid level detection is correctly performed, and waste of the sample, the reagent, and the like can be prevented.
なお、本発明の他の実施例としては、互いに離間した
１対の電極部と試料採取ノズルとを並設して支持する液
面検知センサを使用し、２本の電極間の電気伝導を測定
することによって、第１の実施例と同様に血液凝固成分
の付着を検出することができる。As another embodiment of the present invention, a liquid level detection sensor that supports a pair of electrode parts and a sampling nozzle which are separated from each other and supports them side by side is used to measure the electric conduction between two electrodes. Thus, the adhesion of the blood coagulation component can be detected in the same manner as in the first embodiment.
以上説明した如く、本発明の血液自動採取方法によれ
ば、試料採取ノズルと電極と間の血液凝固成分の付着を
検出し、この血液凝固成分を除去する。これにより、血
液試料の液面検知の誤動作を略完全に防ぐことができ
る。この結果、所定量の血液試料を適切に自動採取する
ことを容易に達成できる等効果を奏するものである。As described above, according to the automatic blood sampling method of the present invention, the adhesion of the blood coagulation component between the sample collection nozzle and the electrode is detected, and the blood coagulation component is removed. As a result, it is possible to almost completely prevent the malfunction of the liquid level detection of the blood sample. As a result, it is possible to easily achieve appropriate automatic collection of a predetermined amount of blood sample.
第１図は、本発明の実施例で使用した血液自動採取装置
の構成を示す説明図、第２図及び第３図は、同実施例で
使用する洗浄ステーションの要部を示す説明図である。 １……血液自動採取装置、２……試料採取ノズル、３…
…電極、４……液面検知センサ、８……信号増巾器、９
……信号比較器、10……第１の信号変換器、11……分析
機、13……データプロセッサ、15……第２の信号変換
器、16……表示装置、17……ブザー、18……試料容器、
19……血液試料、20……血液凝固成分、30、40……洗浄
ステーション。FIG. 1 is an explanatory view showing a configuration of an automatic blood sampling apparatus used in an embodiment of the present invention, and FIGS. 2 and 3 are explanatory views showing a main part of a washing station used in the embodiment. . 1 ... automatic blood sampling device, 2 ... sample sampling nozzle, 3 ...
... Electrode, 4 ... Liquid level detection sensor, 8 ... Signal amplifier, 9
... Signal comparator, 10 first signal converter, 11 analyzer, 13 data processor, 15 second signal converter, 16 display device, 17 buzzer, 18 …… Sample container,
19 blood sample, 20 blood coagulation component, 30, 40 washing station.
フロントページの続き (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 1/00 101 G01N 35/00 - 35/10 Continuation of front page (58) Field surveyed (Int.Cl. 7 , DB name) G01N 1/00 101 G01N 35/00-35/10
Claims (2)
に離間し、かつ並列して支持する液面検知センサを試料
容器に向かって下降させる工程と、 前記試料用ノズルと前記電極部の間への血液検体の侵入
による両部材間の電気伝導の変化により、前記試料採取
ノズルの先端部が前記血液試料の液面に到達したことを
検知して前記試料採取ノズルの先端部を該血液試料の所
定の深さで停止させる工程と、 試料採取作業終了後、次の血液検体の採取を開始する前
に、前記試料採取ノズル及び電極間の電気伝導の有無を
測定し、両部材間に付着した前記血液凝固成分の付着を
検出する工程を具備することを特徴とする血液自動採取
方法。A step of lowering a liquid level detection sensor supporting both a sample collection nozzle and an electrode portion toward each other and supporting the sample collection nozzle and the electrode portion in parallel to a sample container; A change in electric conduction between the two members due to invasion of the blood sample into the space detects that the tip of the sample collection nozzle has reached the liquid level of the blood sample, and changes the tip of the sample collection nozzle to the blood. A step of stopping at a predetermined depth of the sample, after the end of the sample collection operation, before starting the next blood sample collection, measuring the presence or absence of electrical conduction between the sample collection nozzle and the electrode, between the two members A method for automatically collecting blood, comprising a step of detecting the adhesion of the attached blood coagulation component.
ズルとを並設して支持する液面検知センサを、試料容器
に収容された血液試料に向かって下降させる工程と、前
記電極部の間への血液検体の侵入による両部材間の電気
伝導の変化により、前記試料採取ノズルの先端部が前記
血液試料の液面に到達したことを検知して前記試料採取
ノズルの先端部を該血液試料の所定の深さで停止させる
工程と、 試料採取作業了後、次の血液検体の採取を開始する前
に、前記試料採取ノズル及び電極間の電気伝導の有無を
測定し、両部材間に付着した前記血液凝固成分の付着を
検出する工程を具備することを特徴とする血液自動採取
方法。2. A step of lowering a liquid level detection sensor supporting a pair of electrode portions and a sample collection nozzle arranged side by side toward a blood sample contained in a sample container; The change in electrical conduction between the two members due to the invasion of the blood sample into the gap detects that the tip of the sample collection nozzle has reached the liquid level of the blood sample, and changes the tip of the sample collection nozzle. A step of stopping at a predetermined depth of the blood sample, and after completion of the sample collection operation, before starting collection of the next blood sample, measuring the presence or absence of electrical conduction between the sample collection nozzle and the electrode. A method of detecting the adhesion of the blood coagulation component adhered to the blood.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22866690A JP3085967B2 (en) | 1990-08-30 | 1990-08-30 | Automatic blood collection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22866690A JP3085967B2 (en) | 1990-08-30 | 1990-08-30 | Automatic blood collection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04110634A JPH04110634A (en) | 1992-04-13 |
| JP3085967B2 true JP3085967B2 (en) | 2000-09-11 |
Family
ID=16879913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22866690A Expired - Lifetime JP3085967B2 (en) | 1990-08-30 | 1990-08-30 | Automatic blood collection method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3085967B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016201979A (en) * | 2015-04-07 | 2016-12-01 | サムヤン エスエヌイー カンパニー リミテッド | Anti-perching device for solar modules |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102006052833A1 (en) * | 2006-11-09 | 2008-05-15 | Diasys Diagnostic Systems Gmbh | A method for detecting a blockage, a coagulum or a plug at the receiving opening of a dispensing needle |
| JP6491922B2 (en) * | 2015-03-27 | 2019-03-27 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
-
1990
- 1990-08-30 JP JP22866690A patent/JP3085967B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016201979A (en) * | 2015-04-07 | 2016-12-01 | サムヤン エスエヌイー カンパニー リミテッド | Anti-perching device for solar modules |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04110634A (en) | 1992-04-13 |
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