JP3062082B2 - Settling tube for liquid centrifugation - Google Patents
Settling tube for liquid centrifugationInfo
- Publication number
- JP3062082B2 JP3062082B2 JP8122418A JP12241896A JP3062082B2 JP 3062082 B2 JP3062082 B2 JP 3062082B2 JP 8122418 A JP8122418 A JP 8122418A JP 12241896 A JP12241896 A JP 12241896A JP 3062082 B2 JP3062082 B2 JP 3062082B2
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- container body
- cap
- inner tube
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Description
【０００１】[0001]
【発明の属する技術分野】この発明は、２つもしくはそ
れ以上の液相に分離可能な混合液を、遠心力を利用して
比重の差により分離する場合に使用される遠心分離用沈
殿管（以下、「遠沈管」という）に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a centrifugal sedimentation tube used for separating a liquid mixture which can be separated into two or more liquid phases by a difference in specific gravity using centrifugal force. Hereinafter, referred to as “centrifuge tube”).
【０００２】[0002]
【従来の技術】遠心機を使用して混合液、例えば２つの
液相に分離可能な水と有機溶媒とを比重の差により上層
液と下層液とに分離する場合は、遠沈管が使用される。
従来の遠沈管は、図５に示すように、上面が開口した管
状をなす容器本体１と、この容器本体１の上面開口を液
密に閉塞するキャップ２とから構成されている。この遠
沈管を用いて混合液を上層液と下層液とに分離するに
は、容器本体１に混合液を注入し、容器本体１の上面開
口にキャップ２を密嵌させた後、遠沈管を遠心機にかけ
る。これにより、遠沈管内の混合液は、図５に示すよう
に比重の差で上層液３と下層液４とに分離される。2. Description of the Related Art When a mixed liquid, for example, water and an organic solvent which can be separated into two liquid phases is separated into an upper liquid and a lower liquid by a difference in specific gravity using a centrifuge, a centrifuge tube is used. You.
As shown in FIG. 5, a conventional centrifugal tube includes a tubular container body 1 having an open upper surface, and a cap 2 for closing the upper surface opening of the container body 1 in a liquid-tight manner. In order to separate the mixed solution into an upper layer solution and a lower layer solution using this centrifuge tube, the mixed solution is poured into the container body 1, and the cap 2 is tightly fitted to the upper opening of the container body 1, and then the centrifuge tube is removed. Centrifuge. Thereby, the mixed liquid in the centrifuge tube is separated into the upper liquid 3 and the lower liquid 4 with a difference in specific gravity as shown in FIG.
【０００３】遠心力により遠沈管内で分離された上層液
３と下層液４のうち、下層液４を抽出するには、従来、
シリンジを使用し、吸引ノズルを通し上層液３の全部を
吸引して容器本体１内から排出することにより、容器本
体１内に下層液４だけを残す方法が一般的に行なわれて
いる。また、手作業による場合には、ホールピペッタや
ピペッタなどを使用し、上層液３と下層液４との境界面
を目視で確認しながら上層液３の全部を容器本体１内か
ら除去するようにしている。In order to extract the lower layer liquid 4 from the upper layer liquid 3 and the lower layer liquid 4 separated in the centrifuge tube by centrifugal force, conventionally,
A method is generally used in which a syringe is used to suck all of the upper layer liquid 3 through a suction nozzle and discharge it from the container body 1 so that only the lower layer liquid 4 remains in the container body 1. In the case of manual operation, a whole pipette or pipetter is used to remove the entire upper liquid 3 from the container body 1 while visually checking the boundary surface between the upper liquid 3 and the lower liquid 4. I have.
【０００４】[0004]
【発明が解決しようとする課題】しかしながら、シリン
ジにより吸引ノズルを通し上層液３の全部を吸引して容
器本体１内から取り除く方法では、光電スイッチなどを
使用すれば上層液３と下層液４との境界面の位置を検出
して上層液３だけを容器本体１内から排出することは可
能であるが、吸引ノズルによって上層液３を吸引する際
に液流を生じ、このため、上層液３の一部が下層液４と
混ざり合って下層液４にコンタミネーション（汚染）を
生じる、といった問題点がある。また、ホールピペッタ
等を用いた手作業による方法では、上層液３を下層液４
との境界面付近まで排除したときに、液の種類によって
は上層液３の一部が下層液４と混ざり易くなるので、下
層液４にコンタミネーションを生じないように上層液３
だけを容器本体１内から取り除くには、相当の熟練度を
要する、といった問題点がある。また、このような手作
業による方法を自動化することは、非常に困難である。However, in the method of removing all of the upper liquid 3 from the inside of the container main body 1 by suctioning the upper liquid 3 through a suction nozzle with a syringe, the upper liquid 3 and the lower liquid 4 can be separated by using a photoelectric switch or the like. Although it is possible to detect only the upper layer liquid 3 from the inside of the container body 1 by detecting the position of the boundary surface of the upper layer liquid, a liquid flow occurs when the upper layer liquid 3 is sucked by the suction nozzle. Is mixed with the lower layer liquid 4 to cause contamination of the lower layer liquid 4. In the manual method using a hole pipettor or the like, the upper liquid 3 is replaced with the lower liquid 4.
When the liquid is removed up to the vicinity of the boundary with the lower liquid 4, depending on the type of liquid, a part of the upper liquid 3 is easily mixed with the lower liquid 4, so that the upper liquid 3 is not contaminated with the lower liquid 4.
There is a problem that a considerable degree of skill is required to remove only from the container body 1. Further, it is very difficult to automate such a manual method.
【０００５】この発明は、以上のような事情に鑑みてな
されたものであり、遠心力により上層液と下層液とに分
離された２液相系（或いはそれ以上の液相系）から、全
くコンタミネーションを生じることなく下層液だけを抽
出することができ、その抽出操作を手作業によって行な
う場合には、殆んど熟練度を要することが無く、また、
その抽出操作を自動化することも容易になるような遠沈
管を提供することを目的とする。[0005] The present invention has been made in view of the above-described circumstances, and is based on a two-liquid system (or more liquid system) separated into an upper liquid and a lower liquid by centrifugal force. It is possible to extract only the lower layer liquid without causing contamination, and when performing the extraction operation by hand, almost no skill is required, and
It is an object of the present invention to provide a centrifuge tube in which the extraction operation can be easily automated.
【０００６】[0006]
【課題を解決するための手段】請求項１に係る発明は、
上面が開口した管状をなす容器本体の上面開口部に被さ
れるキャップを、中央部に貫通孔を有し前記容器本体の
上端部に密嵌する密栓部と、前記容器本体の内径寸法よ
り小さい外径寸法を有する管状をなし、上端部が前記密
栓部の貫通孔部に連接し、容器本体の上端部に密栓部を
密嵌させたときに下端が容器本体の内底面付近に位置す
る程度の長さに形成された内管部と、この内管部の下端
を液密に閉塞し、かつ、下向きの押圧力によって容易に
脱落もしくは破裂する閉塞部とから構成したことを特徴
とする。The invention according to claim 1 is
A cap which covers a top opening of a tubular container body having an open top, a through hole in the center, a tightly plugged portion which is fitted closely to an upper end of the container body, and a smaller inner diameter than the inner diameter of the container body. It is formed in a tubular shape having an outer diameter dimension, and the upper end is connected to the through hole of the hermetic plug, and the lower end is located near the inner bottom surface of the container main body when the hermetic plug is closely fitted to the upper end of the container main body. , And a closing portion that closes the lower end of the inner tube portion in a liquid-tight manner and easily falls off or bursts by a downward pressing force.
【０００７】請求項２に係る発明は、請求項１記載の遠
沈管において、上記キャップの閉塞部を、上記内管部の
下端口に差し込まれる詰め栓としたことを特徴とする。According to a second aspect of the present invention, in the centrifuge tube according to the first aspect, the closing portion of the cap is a filling plug inserted into a lower end of the inner tube portion.
【０００８】上記した構成の請求項１に係る発明の遠沈
管は、その容器本体内に遠心力で分離しようとする液体
を注入した後、キャップの内管部が容器本体内に深く差
し入れられ液体中に挿入されるようにして、密栓部を容
器本体の上端部に密嵌させ、この状態で遠心機にかけら
れる。これにより、遠沈管内の液体は、比重の差によっ
て上層液と下層液とに分離される。このとき、キャップ
の内管部は、容器本体内の液体中に挿入されてその下端
が容器本体の内底面付近に位置しているので、内管部の
下端は、液密に閉塞されて上層液と下層液との境界面よ
り下方に位置し、内管部の下端付近は下層液中に挿入さ
れた状態になっている。このような状態の遠沈管から下
層液だけを抽出するには、分注ノズル（手作業による場
合はピペッタ等。以下では、分注ノズルを用いたとして
説明する）の下端部を、キャップの密栓部の貫通孔を通
り内管部の内方へ深く差し入れ、分注ノズルの下端で内
管部下端の閉塞部を下向きに押圧する。これにより、内
管部の下端を閉塞している閉塞部が脱落もしくは破裂
し、分注ノズルの下端が下層液中に挿入される。この
後、シリンジを駆動させるなどして分注ノズル内へ液体
を吸入すると、分注ノズルの下端は下層液中に挿入され
ているため、下層液だけが分注ノズル内へ吸入される。
そして、内管部の下端、従って分注ノズルの下端は上層
液と下層液との境界面より下方に位置しているため、上
層液の一部が下層液と混ざり合って下層液にコンタミネ
ーションを生じる心配が全く無い。In the centrifuge tube according to the first aspect of the present invention, the liquid to be separated by centrifugal force is injected into the container body, and then the inner tube portion of the cap is inserted deeply into the container body. The hermetic plug is tightly fitted to the upper end of the container body so as to be inserted into the container, and is then centrifuged in this state. Thus, the liquid in the centrifuge tube is separated into an upper liquid and a lower liquid by a difference in specific gravity. At this time, the inner tube portion of the cap is inserted into the liquid in the container body and its lower end is located near the inner bottom surface of the container body, so that the lower end of the inner tube portion is liquid-tightly closed and the upper layer is closed. It is located below the boundary surface between the liquid and the lower layer liquid, and the lower end of the inner pipe portion is inserted into the lower layer liquid. In order to extract only the lower layer liquid from the centrifuge tube in such a state, the lower end of a dispensing nozzle (a pipetter or the like when manually operated; described below using a dispensing nozzle) is sealed with a closed cap. The pipe is inserted deeply into the inner pipe through the through hole of the pipe, and the lower end of the dispensing nozzle presses the closed portion at the lower end of the inner pipe downward. As a result, the closing part closing the lower end of the inner pipe part falls off or bursts, and the lower end of the dispensing nozzle is inserted into the lower liquid. Thereafter, when the liquid is sucked into the dispensing nozzle by driving the syringe or the like, only the lower layer liquid is sucked into the dispensing nozzle because the lower end of the dispensing nozzle is inserted into the lower layer liquid.
Since the lower end of the inner tube, and thus the lower end of the dispensing nozzle, is located below the boundary between the upper liquid and the lower liquid, a part of the upper liquid mixes with the lower liquid and contaminates the lower liquid. There is no need to worry.
【０００９】請求項２に係る発明の遠沈管では、分注ノ
ズルの下端部を、キャップの密栓部の貫通孔を通って内
管部の内方へ深く差し入れ、内管部の下端口に差し込ま
れて内管部下端を閉塞している詰め栓を分注ノズル下端
で下向きに押圧することにより、内管部の下端から詰め
栓が脱落して、分注ノズルの下端が下層液中に挿入され
る。[0009] In the centrifuge tube according to the second aspect of the invention, the lower end of the dispensing nozzle is inserted deeply into the inner tube through the through-hole of the sealed portion of the cap, and inserted into the lower end of the inner tube. When the filling plug closing the lower end of the inner tube is pressed downward by the lower end of the dispensing nozzle, the filling plug drops off from the lower end of the inner tube and the lower end of the dispensing nozzle is inserted into the lower liquid. Is done.
【００１０】[0010]
【発明の実施の形態】以下、この発明の実施形態につい
て図面を参照しながら説明する。Embodiments of the present invention will be described below with reference to the drawings.
【００１１】図１及び図２は、この発明の１実施形態を
示し、図１は、遠沈管を容器本体とキャップとに分離し
た状態を示す斜視図であり、図２は、容器本体にキャッ
プを装着した状態の縦断面図である。1 and 2 show an embodiment of the present invention. FIG. 1 is a perspective view showing a state in which a centrifuge tube is separated into a container body and a cap. FIG. It is a longitudinal cross-sectional view of the state which attached.
【００１２】この遠沈管は、上面が開口した管状をなす
容器本体１０と、この容器本体１０の上面開口を液密に
閉塞するキャップ１２とから構成されている。キャップ
１２は、密栓部１４と内管部１６と閉塞部１８とから構
成されている。密栓部１４は、容器本体１０の上端部に
差し込まれて外周面が密嵌し、中央部に貫通孔２０が形
成されている。内管部１６は、容器本体１０の内径寸法
より小さい外径寸法を有し下部が次第に細径に形成され
た管状をなしており、その上端部が密栓部１４の貫通孔
２０の内周部に固着されて密栓部１４と一体化されてい
る。また、内管部１６は、容器本体１０の上端部に密栓
部１４を密嵌させたときに下端が容器本体１０の内底面
付近に位置する程度の長さに形成されている。閉塞部１
８は、内管部１６の下端口に上向きに差し込まれる詰め
栓によって形成されており、内管部１６の下端を液密に
閉塞している。この詰め栓からなる閉塞部１８は、下向
きの押圧力、すなわち分注ノズルの下端によって下向き
に押し付けられる力により容易に脱落するようになって
いる。This centrifuge tube comprises a tubular container body 10 having an open upper surface, and a cap 12 for closing the upper surface opening of the container body 10 in a liquid-tight manner. The cap 12 includes a hermetic plug portion 14, an inner tube portion 16, and a closing portion 18. The hermetic plug portion 14 is inserted into the upper end portion of the container main body 10 and has an outer peripheral surface closely fitted thereto, and a through hole 20 is formed in a central portion. The inner tube portion 16 has an outer diameter smaller than the inner diameter of the container body 10, and has a tubular shape in which a lower portion is gradually reduced in diameter, and an upper end portion thereof is formed in an inner peripheral portion of the through hole 20 of the plug portion 14. And is integrated with the sealing portion 14. The inner tube portion 16 is formed to have a length such that the lower end thereof is located near the inner bottom surface of the container body 10 when the stopper 14 is closely fitted to the upper end portion of the container body 10. Closed part 1
Numeral 8 is formed by a filling plug that is inserted upward into the lower end of the inner tube 16, and closes the lower end of the inner tube 16 in a liquid-tight manner. The closing portion 18 made of the filling plug is easily dropped by a downward pressing force, that is, a force pressed downward by the lower end of the dispensing nozzle.
【００１３】次に、上記した構成の遠沈管を使用し、混
合液を上層液と下層液とに分離して下層液のみを抽出す
る方法について、図３を参照しながら説明する。Next, a method for separating the mixed solution into an upper layer solution and a lower layer solution and extracting only the lower layer solution by using the centrifuge tube having the above structure will be described with reference to FIG.
【００１４】まず、キャップ１２を外した状態で本体容
器１０内に混合液を注入した後、内管部１６が容器本体
１０内に深く差し入れられて液体中に挿入されるように
し、密栓部１４が容器本体１０の上端部に密嵌されるよ
うにして、キャップ１２を容器本体１０に装着する。こ
の状態で遠沈管を遠心機にかけることにより、図３に示
すように、容器本体１０の内周面とキャップ１２の内管
部１６の外周面との間に収容された液体が上層液２２と
下層液２４とに分離される。このとき、図３の（ａ）に
示すように、キャップ１２の内管部１６は、その下端が
容器本体１０の内底面付近に位置しているので、内管部
１６の下端は、上層液２２と下層液２４との境界面２６
より下方に位置している。このため、内管部１６の下端
付近は、下層液２４中に挿入された状態になっている。First, after the mixed liquid is poured into the main body container 10 with the cap 12 removed, the inner tube portion 16 is inserted deeply into the container main body 10 so as to be inserted into the liquid. The cap 12 is attached to the container body 10 such that the cap 12 is tightly fitted to the upper end of the container body 10. By centrifuging the centrifuge tube in this state, as shown in FIG. 3, the liquid contained between the inner peripheral surface of the container body 10 and the outer peripheral surface of the inner tube portion 16 of the cap 12 becomes the upper layer liquid 22. And the lower layer liquid 24. At this time, as shown in FIG. 3A, since the lower end of the inner tube 16 of the cap 12 is located near the inner bottom surface of the container body 10, the lower end of the inner tube 16 is Interface 26 between lower fluid 22 and lower layer liquid 24
It is located below. Therefore, the vicinity of the lower end of the inner pipe portion 16 is in a state of being inserted into the lower layer liquid 24.
【００１５】次に、自動分注装置（全体の構成は図示せ
ず）により遠沈管から下層液だけを抽出するには、遠沈
管を自動分注装置の取付け部（図示せず）に固定する。
続いて、ノズル駆動機構（図示せず）により、図３の
（ａ）に示すように分注ノズル２８を下降させ、図３の
（ｂ）に示すように分注ノズル２８の下端部をなしてい
るディスポーザブルチップ（以下、「ディスポチップ」
という）３０を、遠沈管のキャップ１２の密栓部１４の
貫通孔２０を通って内管部１６の内方へ深く差し入れ
る。そして、ディスポチップ３０の下端で内管部１６下
端の閉塞部１８を下向きに押圧することにより、図３の
（ｃ）に示すように、詰め栓からなる閉塞部１８を内管
部１６の下端から脱落させ、ディスポチップ３０の下端
を下層液２４中に挿入させる。この後、分注ノズル２８
に接続されているシリンジ（図示せず）を駆動させるこ
とにより、ディスポチップ３０の下端口を通ってディス
ポチップ３０内に液体を吸入する。この際、ディスポチ
ップ３０の下端は下層液２４中に挿入されているため、
下層液２４だけがディスポチップ３０内へ吸入され、ま
た、ディスポチップ３０の下端は上層液２２と下層液２
４との境界面２６よりずっと下方に位置しているため、
上層液２２の一部が下層液２４と混ざり合ってコンタミ
ネーションを生じる、といった心配は全く無い。Next, in order to extract only the lower layer liquid from the centrifuge tube by an automatic dispensing device (the whole configuration is not shown), the centrifuge tube is fixed to a mounting portion (not shown) of the automatic dispensing device. .
Subsequently, the dispensing nozzle 28 is lowered by a nozzle driving mechanism (not shown) as shown in FIG. 3A, and the lower end of the dispensing nozzle 28 is formed as shown in FIG. 3B. Disposable chips (hereinafter referred to as “disposable chips”)
) 30 is inserted deeply into the inner tube portion 16 through the through hole 20 of the plug portion 14 of the cap 12 of the centrifuge tube. Then, the lower end of the disposable tip 30 presses the closing portion 18 at the lower end of the inner tube portion 16 downward, thereby closing the closing portion 18 formed of a filling plug as shown in FIG. , And the lower end of the disposable chip 30 is inserted into the lower layer liquid 24. Thereafter, the dispensing nozzle 28
By driving a syringe (not shown) connected to the dispenser chip 30, a liquid is sucked into the disposable chip 30 through the lower end opening of the disposable chip 30. At this time, since the lower end of the disposable chip 30 is inserted into the lower layer liquid 24,
Only the lower layer liquid 24 is sucked into the disposable chip 30, and the lower end of the disposable chip 30 is connected to the upper layer liquid 22 and the lower layer liquid 2.
Because it is located much lower than the boundary surface 26 with 4,
There is no concern that a part of the upper liquid 22 is mixed with the lower liquid 24 to cause contamination.
【００１６】図４に縦断面図を示した遠沈管は、キャッ
プ３２の密栓部３４が、容器本体１０の上端に被せられ
て液密に外嵌する構造を有し、その密栓部３４の貫通孔
４０の内周部に内管部３６の上端部が固着されて、密栓
部３４と内管部３６とが一体化されている。また、内管
部３６の下端に薄板状部が一体形成されて閉塞部３８を
成している。この薄板状部からなる閉塞部３８は、分注
ノズルの下端によって下向きに押し付けられることによ
り、容易に破裂するようになっている。The centrifugal tube whose longitudinal section is shown in FIG. 4 has a structure in which the hermetically sealed portion 34 of the cap 32 is placed over the upper end of the container body 10 and externally fits in a liquid-tight manner. The upper end of the inner tube portion 36 is fixed to the inner peripheral portion of the hole 40, so that the plug portion 34 and the inner tube portion 36 are integrated. Further, a thin plate portion is integrally formed at the lower end of the inner tube portion 36 to form a closing portion 38. The closing portion 38 formed of the thin plate portion is easily ruptured by being pressed downward by the lower end of the dispensing nozzle.
【００１７】尚、上記各実施形態では、キャップ１２、
３２を構成する密栓部１４、３４と内管部１６、３６と
が別体とされ、密栓部１４、３４に内管部１６、３６を
固着してそれらを一体化しているが、密栓部と内管部と
を一体形成するようにしてもよい。また、内管部の下端
を液密に閉塞し下向きの押圧力によって容易に脱落もし
くは破裂する閉塞部の構成は、上記実施形態で示した詰
め栓や内管部下端に薄板状部を一体形成したものに限ら
ず、例えば内管部の下端口をフィルムで被覆して液密に
閉塞するような構成であってもよい。また、図３に基づ
いた上記説明では、自動分注装置により遠沈管から下層
液だけを抽出するようにしているが、ホールピペッタ等
を使用して手作業により遠沈管から下層液だけを抽出す
ることも、勿論可能である。In each of the above embodiments, the cap 12,
Although the hermetic plug portions 14 and 34 and the inner tube portions 16 and 36 which constitute 32 are separate from each other, and the inner tube portions 16 and 36 are fixed to the hermetic plug portions 14 and 34 to integrate them, You may make it integrally form with an inner tube part. In addition, the configuration of the closing portion that closes the lower end of the inner pipe portion in a liquid-tight manner and easily falls or ruptures by a downward pressing force is formed by integrally forming a thin plate-shaped portion at the lower end of the filling plug or the inner pipe portion described in the above embodiment. The present invention is not limited to this, and may have a configuration in which, for example, the lower end of the inner tube portion is covered with a film and closed in a liquid-tight manner. Further, in the above description based on FIG. 3, only the lower layer liquid is extracted from the centrifuge tube by the automatic dispensing device. However, only the lower layer liquid is extracted from the centrifuge tube manually by using a hole pipettor or the like. Of course, it is also possible.
【００１８】[0018]
【発明の効果】請求項１に係る発明の遠沈管を使用すれ
ば、遠心力により上層液と下層液とに分離された２液相
系或いはそれ以上の液相系から、上層液とのコンタミネ
ーションを全く生じることなく下層液だけを抽出するこ
とができる。そして、その抽出操作を手作業によって行
なう場合には、殆んど熟練度を要することが無く、一
方、その抽出操作を自動的に行なおうとする場合には、
その自動化が容易である。According to the centrifuge tube according to the first aspect of the present invention, contamination of the upper liquid from the two liquid phase system or more liquid phase system separated into the upper liquid and the lower liquid by centrifugal force is achieved. Only the lower layer liquid can be extracted without producing any nation. When the extraction operation is performed manually, almost no skill is required. On the other hand, when the extraction operation is to be performed automatically,
Its automation is easy.
【００１９】請求項２に係る発明の遠沈管では、分注ノ
ズルやピペッタ等の下端部をキャップの内管部の内方へ
深く差し入れて分注ノズル等の下端で閉塞部を下向きに
押圧することにより、詰め栓からなる閉塞部が容易に脱
落して、分注ノズル等の下端を下層液中に挿入すること
ができ、下層液だけの抽出が可能になる。In the centrifuge tube according to the second aspect of the invention, the lower end of the dispensing nozzle or pipettor is inserted deeply into the inner tube of the cap, and the closed portion is pressed downward by the lower end of the dispensing nozzle or the like. As a result, the closing portion formed by the filling plug easily falls off, and the lower end of the dispensing nozzle or the like can be inserted into the lower layer liquid, and only the lower layer liquid can be extracted.
【図１】この発明の１実施形態である遠沈管を容器本体
とキャップとに分離した状態を示す斜視図である。FIG. 1 is a perspective view showing a state in which a centrifuge tube according to one embodiment of the present invention is separated into a container body and a cap.
【図２】図１に示した遠沈管の容器本体にキャップを装
着した状態の縦断面図である。FIG. 2 is a longitudinal sectional view showing a state in which a cap is attached to the container main body of the centrifuge tube shown in FIG.
【図３】図１及び図２に示した遠沈管を使用し、混合液
を上層液と下層液とに分離して下層液のみを抽出する操
作について説明するための縦断面図である。FIG. 3 is a longitudinal sectional view for explaining an operation of using the centrifuge tube shown in FIGS. 1 and 2 to separate a mixed solution into an upper liquid and a lower liquid and extract only a lower liquid.
【図４】この発明の別の実施形態を示す遠沈管の縦断面
図である。FIG. 4 is a longitudinal sectional view of a centrifuge tube showing another embodiment of the present invention.
【図５】従来の遠沈管の縦断面図である。FIG. 5 is a longitudinal sectional view of a conventional centrifuge tube.
１０ 遠沈管の容器本体 １２、３２ 遠沈管のキャップ １４、３４ キャップの密栓部 １６、３６ キャップの内管部 １８、３８ キャップの閉塞部 ２０、４０ 密栓部の貫通孔 ２２ 上層液 ２４ 下層液 ２６ 上層液と下層液との境界面 ２８ 分注ノズル DESCRIPTION OF SYMBOLS 10 Container body of centrifuge tube 12, 32 Cap of centrifuge tube 14, 34 Sealing part of cap 16, 36 Inner tube part of cap 18, 38 Capping part of cap 20, 40 Through-hole of sealing part 22 Upper liquid 24 Lower liquid 26 Interface between upper and lower liquids 28 Dispensing nozzle
───────────────────────────────────────────────────── フロントページの続き (72)発明者 西村 伸太郎 大阪府摂津市鶴野４−３−34−517 (72)発明者 村田 正好 大阪府豊能郡豊能町東ときわ台７−７− 17 (56)参考文献 特開 平１−297161（ＪＰ，Ａ) 実開 昭58−48351（ＪＰ，Ｕ) 実開 昭49−65090（ＪＰ，Ｕ) 実開 昭57−131856（ＪＰ，Ｕ) 実開 昭49−32692（ＪＰ，Ｕ) 実公 昭25−4611（ＪＰ，Ｙ１) (58)調査した分野(Int.Cl.7，ＤＢ名) B04B 5/02 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Shintaro Nishimura 4-3-34-517, Tsuruno, Settsu-shi, Osaka (72) Inventor Masayoshi Murata 7-7-17, Higashi Tokiwadai, Toyono-cho, Toyono-gun, Osaka (56) References JP-A-1-297161 (JP, A) JP-A-58-48351 (JP, U) JP-A-49-65090 (JP, U) JP-A-57-131856 (JP, U) JP-A-49-32692 (JP, U) J. 25-1461 (JP, Y1) (58) Field surveyed (Int. Cl. 7 , DB name) B04B 5/02
Claims (2)
この容器本体の上面開口部に被されるキャップとからな
る、液体の遠心分離用沈殿管において、 前記キャップを、 中央部に貫通孔を有し、前記容器本体の上端部に密嵌す
る密栓部と、 前記容器本体の内径寸法より小さい外径寸法を有する管
状をなし、上端部が前記密栓部の貫通孔部に連接し、容
器本体の上端部に密栓部を密嵌させたときに下端が容器
本体の内底面付近に位置する程度の長さに形成された内
管部と、 この内管部の下端を液密に閉塞し、かつ、下向きの押圧
力によって容易に脱落もしくは破裂する閉塞部とから構
成したことを特徴とする、液体の遠心分離用沈殿管。A tubular container body having an open upper surface;
In a sedimentation tube for centrifugal separation of a liquid, comprising a cap covered on an opening on the upper surface of the container body, the cap having a through hole in a central portion, and a tightly plug portion which is closely fitted to an upper end portion of the container body. And a tubular shape having an outer diameter smaller than the inner diameter of the container body, the upper end is connected to the through hole of the plug, and the lower end is tightly fitted when the plug is tightly fitted to the upper end of the container. An inner tube formed to have a length near the inner bottom surface of the container body; and a closing portion that closes the lower end of the inner tube in a liquid-tight manner and easily falls off or bursts by a downward pressing force. A sedimentation tube for centrifugal separation of a liquid, comprising:
差し込まれる詰め栓である請求項１記載の、液体の遠心
分離用沈殿管。2. The sedimentation tube for centrifugal separation of liquid according to claim 1, wherein the closing portion is a filling plug inserted into a lower end of an inner tube portion of the cap.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8122418A JP3062082B2 (en) | 1996-04-19 | 1996-04-19 | Settling tube for liquid centrifugation |
| PCT/JP1997/001366 WO1997040357A1 (en) | 1996-04-19 | 1997-04-18 | Automatic extracting equipment and automatic concentration measuring equipment for component substance in liquid sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8122418A JP3062082B2 (en) | 1996-04-19 | 1996-04-19 | Settling tube for liquid centrifugation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH09285740A JPH09285740A (en) | 1997-11-04 |
| JP3062082B2 true JP3062082B2 (en) | 2000-07-10 |
Family
ID=14835342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8122418A Expired - Fee Related JP3062082B2 (en) | 1996-04-19 | 1996-04-19 | Settling tube for liquid centrifugation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3062082B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005030399A1 (en) * | 2003-09-30 | 2005-04-07 | Kabushiki Kaisha Kitazato Supply | Settling tube for centrifugal separation |
| WO2009054441A1 (en) | 2007-10-24 | 2009-04-30 | Jms Co., Ltd. | Separation container, attachment and separation method |
| JP5054161B2 (en) * | 2010-06-29 | 2012-10-24 | 株式会社 ユニフローズ | Extraction container, extraction apparatus and extraction method using the same |
| AU2011327246A1 (en) * | 2010-11-09 | 2013-05-02 | Jms Co., Ltd. | Separation container and separation method |
-
1996
- 1996-04-19 JP JP8122418A patent/JP3062082B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH09285740A (en) | 1997-11-04 |
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