JP3058766B2 - Sample measuring method and sample measuring device - Google Patents
Sample measuring method and sample measuring deviceInfo
- Publication number
- JP3058766B2 JP3058766B2 JP4190063A JP19006392A JP3058766B2 JP 3058766 B2 JP3058766 B2 JP 3058766B2 JP 4190063 A JP4190063 A JP 4190063A JP 19006392 A JP19006392 A JP 19006392A JP 3058766 B2 JP3058766 B2 JP 3058766B2
- Authority
- JP
- Japan
- Prior art keywords
- carrier particles
- gap
- sample
- substance
- measuring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Description
【０００１】[0001]
【産業上の利用分野】本発明は、検体中の複数免疫学的
活性物質を定性的又は定量的に検出する検体測定方法及
び検体測定装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sample measuring method and a sample measuring device for qualitatively or quantitatively detecting a plurality of immunologically active substances in a sample.
【０００２】[0002]
【従来の技術】検体中の抗原、抗体等の免疫学的活性物
質を検出する方法としては、ラテックス粒子、ガラス粒
子、セラミック球、カオリン、カーボンブラック、赤血
球等の動物血液成分等のコロイド粒子等の担体粒子に免
疫学的活性物質を感作させ、その担体粒子を液体媒体中
で検体と反応させて、反応液の凝集状態を検者が肉眼で
観察、確認して、免疫学的活性物質を定性的に検出する
方法がよく知られている。2. Description of the Related Art Methods for detecting immunologically active substances such as antigens and antibodies in a specimen include colloid particles such as latex particles, glass particles, ceramic spheres, animal blood components such as kaolin, carbon black, and red blood cells. The carrier particles are sensitized with an immunologically active substance, the carrier particles are allowed to react with a sample in a liquid medium, and the examiner visually observes and confirms the aggregation state of the reaction solution. Methods for qualitatively detecting are well known.
【０００３】[0003]
【発明が解決しようとする課題】しかしながら上述の従
来例においては、凝集状態を肉眼で判断する場合には、
定量性に乏しい検出しかできず、検出結果の精度、信頼
性を欠いている。また、検出対象について１項目の測定
しかできず、検査項目が拡大するにつれ、同時に多項目
検出が可能な技術が求められている。However, in the above-mentioned conventional example, when the state of aggregation is visually determined,
It can only perform detection with poor quantitativeness, and lacks the accuracy and reliability of detection results. In addition, only one item can be measured for a detection target, and as the number of inspection items increases, a technique capable of simultaneously detecting multiple items is required.
【０００４】本発明の目的は、検体中の複数種の免疫学
的活性物質を同時に検出するための、簡便で精度の高い
検体測定方法及び検体測定装置を提供することにある。An object of the present invention is to provide a simple and accurate sample measuring method and a sample measuring apparatus for simultaneously detecting a plurality of types of immunologically active substances in a sample.
【０００５】[0005]
【課題を解決するための手段】上述の目的を達成するた
めの第１発明に係る検体測定方法は、特定物質と結合す
る物質を担持させた担体粒子と検体との反応液中におけ
る前記担体粒子の凝集の程度により、検体中の前記特定
物質の測定を行う検体測定方法において、第１の特定物
質と結合する第１の物質を担持させた第１の担体粒子、
第２の特定物質と結合する第２の物質を担持させ前記第
１の担体粒子とは異なる種類の第２の担体粒子、検体を
液体媒体中で反応させて反応液を得る第１の工程と、該
反応液中の担体粒子がその凝集径に応じた間隔を持った
間隙位置に挟まれてトラップされるように最大間隔から
間隔が徐々に減少する間隙部に前記第１の工程で得られ
る反応液を注入する第２の工程と、前記第１及び第２の
担体粒子が前記間隙部中で挟まれてトラップされる位置
を基に検体中の前記第１及び第２の特定物質をそれぞれ
区別して測定する第３の工程とを有することを特徴とす
る。According to a first aspect of the present invention, there is provided a method for measuring a specimen, the method comprising the steps of: In the sample measurement method for measuring the specific substance in the sample depending on the degree of aggregation, the first carrier particles carrying the first substance that binds to the first specific substance,
A first step of supporting a second substance that binds to a second specific substance and reacting a second carrier particle of a type different from the first carrier particle and a sample in a liquid medium to obtain a reaction solution; In the first step, the carrier particles in the reaction solution are obtained in the gap portion where the interval gradually decreases from the maximum interval so as to be trapped by being interposed between the gap positions having an interval corresponding to the aggregation diameter. A second step of injecting the reaction solution, and the first and second specific substances in the sample are respectively based on the positions where the first and second carrier particles are trapped by being sandwiched in the gap. And a third step of measuring separately.
【０００６】また、第２発明に係る検体測定装置は、特
定物質と結合する物質を担持させた担体粒子と検体との
反応液中における前記担体粒子の凝集の程度により、検
体中の前記特定物質の測定を行う検体測定装置におい
て、複数の扇型セクタに分割した円板形状を有し、これ
らの各セクタでは中心から外側に向かって一様に又は段
階的に減少した間隙部を有し、少なくとも前記間隙部の
最大間隔部及び最小間隔部の２端を開口し、前記間隙部
を囲む少なくとも１面が透明であり、最大間隔が担体粒
子の径よりも十分大きく、最小間隔が担体粒子の径より
も小さいことを特徴とするものである。Further, the sample measuring apparatus according to the second invention is characterized in that the specific substance in the sample is determined by the degree of aggregation of the carrier particles in the reaction solution between the sample and the carrier particles carrying the substance that binds to the specific substance. In the sample measurement device that performs the measurement, has a disk shape divided into a plurality of sector-shaped sectors, in each of these sectors has a gap that decreases uniformly or stepwise from the center to the outside, At least two ends of the maximum interval and the minimum interval of the gap are opened, at least one surface surrounding the gap is transparent, the maximum interval is sufficiently larger than the diameter of the carrier particles, and the minimum interval is the length of the carrier particles. It is characterized by being smaller than the diameter.
【０００７】[0007]
【作用】上述の構成を有する第１発明に係る検体測定方
法は、最大間隔部の開口から間隙に反応液を注入する
と、間隔差によって大きさが異なる担体粒子、凝集体、
液体媒体等が分離され、凝集程度を判別識別できる。In the sample measuring method according to the first aspect of the present invention having the above-described structure, when the reaction solution is injected into the gap from the opening of the maximum interval, the size of the carrier particles, aggregates,
The liquid medium or the like is separated, and the degree of aggregation can be determined and identified.
【０００８】[0008]
【実施例】本発明を図示の実施例に基づいて詳細に説明
する。図１は第１の実施例の試料台１の外観斜視図であ
り、図２は図１のＡ−Ｂ方向の縦断面図である。透明部
材によって形成される平板状の基板２の上には、透明部
材によって形成され、中央内側に凹部３ａを設けた楔状
のカバー部材３が密着され、基板２と凹部３ａにより間
隙が形成されている。この凹部３ａは図２に示すよう
に、間隙の高さがＡ方向からＢ方向へ一様に減少するよ
うにされ、Ｂ方向の端部の開口の垂直間隔DBは使用する
担体粒子の最小径Ｒよりも小さくされており、Ａ方向の
端部の開口の垂直間隔DAは単体粒子が凝集した凝集体Ｇ
も通過できるように、垂直間隔DBの数倍〜数１００倍程
度とされている。DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described in detail with reference to the illustrated embodiments. FIG. 1 is an external perspective view of a sample stage 1 according to the first embodiment, and FIG. 2 is a longitudinal sectional view in the AB direction of FIG. A wedge-shaped cover member 3 formed of a transparent member and provided with a recess 3a at the center inside is in close contact with the flat substrate 2 formed of the transparent member, and a gap is formed by the substrate 2 and the recess 3a. I have. As shown in FIG. 2, the height of the gap is uniformly reduced from the direction A to the direction B, and the vertical interval DB of the opening at the end in the direction B is the minimum diameter of the carrier particles used. R is smaller than R, and the vertical interval DA of the opening at the end in the A direction is the aggregate G in which the single particles are aggregated.
Is set to several times to several hundred times the vertical interval DB so that the vertical distance DB can pass through.
【０００９】担体粒子F1、F2にそれぞれ異なる免疫学的
活性物質を感作させ、これらの担体粒子F1、F2を水を主
体とする液体媒体中に分散させた試薬と検体とを混合す
ると反応が起こり、複数個の免疫学的活性物質と担体粒
子F1、F2とがそれぞれ凝集体G1、G2を形成する。十分に
反応させた後に、図３に示すようにこの反応液Ｌを基板
２と凹部３ａとの間隙にＡ方向から注入すると、表面張
力によって反応液Ｌは垂直間隔の狭いＢ方向へ侵入して
ゆく。未凝集の担体粒子F1、F2は径が小さいのでＢ方向
の奥まで移動できるが、凝集体G1、G2はその大きさに依
存して途中でトラップされて移動できなくなり、大きい
径を持つ凝集体G1、G2から順にＡ−Ｂ方向に分散する。When the carrier particles F1 and F2 are sensitized to different immunologically active substances, and a reagent and a sample in which the carrier particles F1 and F2 are dispersed in a liquid medium mainly composed of water are mixed with a sample, the reaction is carried out. As a result, the plurality of immunologically active substances and the carrier particles F1, F2 form aggregates G1, G2, respectively. After sufficient reaction, the reaction liquid L is injected into the gap between the substrate 2 and the concave portion 3a from the direction A as shown in FIG. go. The unaggregated carrier particles F1 and F2 can move to the back in the B direction because of their small diameter, but the aggregates G1 and G2 are trapped along the way depending on their size and cannot move, and aggregates having a large diameter Disperse in the AB direction in order from G1 and G2.
【００１０】凝集体G1、G2の径は１個の凝集体G1、G2を
構成する担体粒子F1、F2の個数によって定まる。反応に
よって生成した凝集体G1、G2の凝集状態、つまり凝集体
G1、G2を形成する担体粒子F1、F2の個数及び反応液中の
凝集体G1、G2の数は、反応液Ｌ中に含有される２種類の
免疫学的活性物質のそれぞれの性質及び濃度に依存して
いる。従って、間隙に反応液Ｌを流入すると、担体粒子
F1、F2と、凝集体G1、G2がトラップされている位置及び
その数を判別、識別することによって、２種類の免疫学
的活性物質の定性的又は定量的検出を簡単に行うことが
できる。実際には、既知の免疫学的活性物質を含有する
検出量用検体と反応させた反応液Ｌによって予め検量線
を作成しておき、それと比較することによって定量を行
う。The diameter of the aggregates G1 and G2 is determined by the number of carrier particles F1 and F2 constituting one aggregate G1 and G2. Aggregation state of aggregates G1 and G2 generated by reaction, that is, aggregates
The number of carrier particles F1 and F2 forming G1 and G2 and the number of aggregates G1 and G2 in the reaction solution depend on the respective properties and concentrations of the two immunologically active substances contained in the reaction solution L. Depends. Therefore, when the reaction liquid L flows into the gap, the carrier particles
Qualitative or quantitative detection of two types of immunologically active substances can be easily performed by determining and discriminating the positions where F1 and F2 and the aggregates G1 and G2 are trapped and the number thereof. In practice, a calibration curve is prepared in advance by using a reaction solution L reacted with a detection amount sample containing a known immunologically active substance, and quantification is performed by comparing the curve.
【００１１】良好な測定結果を得る工夫として、基板
２、カバー部材３は、何れか一方を担体粒子Ｆの色調と
対照的に着色した不透明部材としてもよく、また例えば
担体粒子Ｆが白色系統の場合には、明度の低い黒色又は
灰色系統の部材によって形成する工夫をして識別を容易
にすることもできる。In order to obtain good measurement results, either the substrate 2 or the cover member 3 may be an opaque member in which one of the substrates 2 and the cover member 3 is colored in contrast to the color tone of the carrier particles F. In such a case, it is also possible to facilitate the identification by devising a black or gray system member having low brightness.
【００１２】また、反応液Ｌが間隙に侵入し易いよう
に、反応液Ｌの液体媒体と親和性の良い物質を間隙の表
面にコートすると、更に良好な測定結果が得られる。コ
ート材としては、液体媒体が水である場合には、親水性
の物質、例えば界面活性剤、メチルセルロース、カルボ
キシメチルセルロース、ポリビニルアルコール、ポリア
クリルアミド等の水溶性高分子が好ましい。Further, when a substance having a good affinity for the liquid medium of the reaction liquid L is coated on the surface of the gap so that the reaction liquid L can easily enter the gap, a better measurement result can be obtained. When the liquid medium is water, the coating material is preferably a hydrophilic substance, for example, a water-soluble polymer such as a surfactant, methylcellulose, carboxymethylcellulose, polyvinyl alcohol, and polyacrylamide.
【００１３】更に、この実施例においては、基板２を水
平に設置して水平方向に反応液Ｌを注入するが、別の使
用例として図１でＡ方向を上にして、基板２を垂直方向
に立てた状態で測定を行ってもよく、重力の効果によっ
て反応液Ｌの侵入が促進され、良好な測定結果が得られ
る。Further, in this embodiment, the substrate 2 is set horizontally and the reaction liquid L is injected in the horizontal direction. As another example of use, the substrate 2 is placed in the vertical direction with the direction A in FIG. The measurement may be performed in a state of standing, and the effect of gravity promotes the penetration of the reaction solution L, and a good measurement result is obtained.
【００１４】図４は重力の効果を利用した試料台１の構
成図を示し、図１の装置を垂直にしたものに対応してお
り、一部を透明とした基台４の内部に下方向に一様に径
が減少する円錐状の間隙部４ａが垂直方向に設けられて
いる。FIG. 4 is a diagram showing the structure of a sample stage 1 utilizing the effect of gravity. The sample stage 1 corresponds to a vertical one of the apparatus shown in FIG. A conical gap portion 4a whose diameter uniformly decreases is provided in the vertical direction.
【００１５】以上の実施例において、粒子径が異なる２
種の担体粒子F1、F2を用いたが、粒子径及び光学特性が
異なる第１、第２の担体粒子F1、F2を用いてもよい。例
えば、光学特性として色調を利用する場合、図５に示す
ように径だけでなく色調が異なっているので、第１、第
２の担体粒子F1、F2の区別がより明瞭になる。In the above examples, particles 2 having different particle diameters were used.
Although the seed carrier particles F1 and F2 are used, first and second carrier particles F1 and F2 having different particle diameters and optical characteristics may be used. For example, when the color tone is used as the optical characteristic, since the color tone is different from the diameter as shown in FIG. 5, the distinction between the first and second carrier particles F1 and F2 becomes clearer.
【００１６】また、光学的特性として蛍光を利用しても
よく、２種の担体粒子F1、F2に異なる波長を持つ蛍光色
素で標識して、蛍光の波長により担体粒子F1、F2を区別
するようにすることもできる。Fluorescence may be used as an optical property. Two kinds of carrier particles F1 and F2 may be labeled with fluorescent dyes having different wavelengths so that carrier particles F1 and F2 are distinguished by the wavelength of fluorescence. You can also
【００１７】更に、担体粒子の粒子径は同一で光学特性
のみを異ならせてもよい。この際に、検体中に第１、第
２の免疫的活性物質が同時に反応液Ｌ中に存在した場合
は、第１、第２の担体粒子F1、F2は間隙中の同一位置に
混在してトラップされるため、定量的な測定にはあまり
向かないが、定性的な測定は十分に可能である。なお、
光学特性以外の識別標識によっても第１、第２の担体粒
子F1、F2を区別でき、例えば磁化粒子、放射性粒子等が
考えられる。Further, the carrier particles may have the same particle diameter and differ only in the optical characteristics. At this time, when the first and second immunologically active substances are simultaneously present in the reaction solution L in the sample, the first and second carrier particles F1 and F2 are mixed at the same position in the gap. Because it is trapped, it is not very suitable for quantitative measurement, but qualitative measurement is sufficiently possible. In addition,
The first and second carrier particles F1 and F2 can also be distinguished by an identification marker other than the optical characteristics, such as magnetized particles and radioactive particles.
【００１８】図６〜図８は第２の実施例であり、図６は
試料台１の斜視図、図７は縦断面図である。基板２と透
明材料から成るカバー部材３の凹部３ｂとの間隙の垂直
間隔がＡ方向からＢ方向に一様に減少し、使用する担体
粒子Ｆの径よりも小さい垂直間隔DBの位置から間隙の幅
が一定とされ、垂直間隔DBの間隙部SBの容積が、垂直間
隔DBよりも垂直間隔の大きい間隙部SAの容積とほぼ等し
いか、それよりも大きくなるようにされている。FIGS. 6 to 8 show a second embodiment. FIG. 6 is a perspective view of the sample stage 1, and FIG. 7 is a longitudinal sectional view. The vertical interval of the gap between the substrate 2 and the concave portion 3b of the cover member 3 made of a transparent material uniformly decreases from the direction A to the direction B, and the gap from the position of the vertical interval DB smaller than the diameter of the carrier particles F to be used. The width is constant, and the volume of the gap SB at the vertical interval DB is set to be approximately equal to or larger than the volume of the gap SA having the vertical interval larger than the vertical interval DB.
【００１９】この実施例においても、第１の実施例とほ
ぼ同様の作用効果が得られ、間隙部SBの容積が大きいた
めに、検出に不必要な混合液の大部分がこの間隙部SBへ
流入し、垂直間隔の大きい間隙部SAではトラップされた
担体粒子Ｆ、凝集体Ｇのみを検出でき、良好な測定結果
を得ることができる。なお、間隔部SBは容積の条件を満
足してれば任意の形状でよい。In this embodiment, substantially the same operation and effect as those of the first embodiment can be obtained, and since the volume of the gap SB is large, most of the mixed liquid unnecessary for detection is supplied to the gap SB. Only the trapped carrier particles F and the aggregates G can be detected in the gap SA having a large vertical interval, and a good measurement result can be obtained. The space SB may have any shape as long as the space condition is satisfied.
【００２０】この場合においても、図８に示すようにＡ
方向を上にして基板２を垂直方向に立てた状態として、
基台４の上側から垂直方向に一様に径が減少し、担体粒
子Ｆの径よりも小さい径DBの位置から断面で直交する２
方向に間隔DBで延在した間隙部SBが設けられ、径DBより
上の位置で担体粒子Ｆをトラップして、不必要な混合液
をその下側の間隙SBに流すことにより良好な測定結果を
得ることができる。この場合に、間隙部SBの形状は容積
が間隙部SAの容積よりも大きければ任意の形状にでき
る。In this case, as shown in FIG.
With the substrate 2 standing upright with the direction up,
The diameter is uniformly reduced in the vertical direction from the upper side of the base 4 and is orthogonal to the cross section from the position of the diameter DB smaller than the diameter of the carrier particles F 2.
A gap SB extending at an interval DB in the direction is provided, and the carrier particles F are trapped at a position above the diameter DB, and an unnecessary mixed solution is caused to flow through the gap SB below the gap SB. Can be obtained. In this case, the shape of the gap SB can be any shape as long as the volume is larger than the volume of the gap SA.
【００２１】図９〜図１５は第３の実施例であり、図９
は円板状の試料台１の平面図である。中心部に開口部を
有し、図１０に示すような扇型から成る４つの領域５
ａ、５ｂ、５ｃ、５ｄに分割されている。図１１は各セ
クタ５の半径方向の断面図であり、図２と同様に透明部
材によって形成される平板状の基板２の上には、透明部
材によって形成され、中央内側に凹部３ａを設けた楔状
のカバー部材３が密着され、凹部３ａにより間隙が形成
されている。垂直間隔DAは円の中心にあり、垂直間隔DB
は弧の部分である。FIGS. 9 to 15 show a third embodiment.
1 is a plan view of a disk-shaped sample stage 1. FIG. Four regions 5 each having an opening in the center and having a fan shape as shown in FIG.
a, 5b, 5c, and 5d. FIG. 11 is a sectional view of each sector 5 in the radial direction. As shown in FIG. 2, on the flat substrate 2 formed by a transparent member, the sector 5 is formed by a transparent member, and a concave portion 3a is provided inside the center. The wedge-shaped cover member 3 is closely attached, and a gap is formed by the concave portion 3a. Vertical spacing DA is at the center of the circle and vertical spacing DB
Is an arc part.
【００２２】図１２は図９のBa-Bc に沿った縦断面図で
ある。セクタ５ａ、５ｃの最大間隔はそれぞれDAa 及び
DAc であり、最小間隔はDBa 、DBc であり、DAc ＞DBc
＞DAa ＞DBa となっている。FIG. 12 is a longitudinal sectional view taken along the line Ba-Bc of FIG. The maximum intervals between sectors 5a and 5c are DAa and
DAc, minimum interval is DBa, DBc, DAc> DBc
>DAa> DBa.
【００２３】開口部から反応液Ｌを注入すると凝集体Ｇ
のうち、DAa 〜DBa の大きさの凝集体Ｇはセクタ５ａで
検出され、DAc 〜DBc の大きさの凝集体Ｇはセクタ５ｃ
で検出されるため、円板の直径の距離で検出範囲が２通
りとなり、検出感度が向上する。更に、DAa 以上の径を
持つ凝集体Ｇはセクタ５ａに入ることはないので目詰ま
りが生ずることはない。When the reaction liquid L is injected from the opening, the aggregate G
Among them, the aggregate G of the size of DAa to DBa is detected in the sector 5a, and the aggregate G of the size of DAc to DBc is detected in the sector 5c.
, The detection range becomes two in accordance with the distance of the diameter of the disk, and the detection sensitivity is improved. Furthermore, since the aggregate G having a diameter equal to or larger than DAa does not enter the sector 5a, clogging does not occur.
【００２４】図１３は扇型セクタ５の間隙部の形状の第
１の変形例であり、半径方向の断面図である。この間隙
部は凹部３ａと基板２との間隙の高さがＡ方向からＢ方
向に４段階に減少するようにされている。この場合に
は、図１４に示すように凝集体Ｇは段階的に分離され
る。FIG. 13 shows a first modification of the shape of the gap of the sector 5 and is a sectional view in the radial direction. In this gap portion, the height of the gap between the concave portion 3a and the substrate 2 is reduced in four steps from the direction A to the direction B. In this case, the aggregate G is separated stepwise as shown in FIG.
【００２５】図１５は扇型セクタ５の第２の変形例であ
り、最大間隔DAから最小間隔DBまで一様に垂直間隔が減
少する間隙部SAと、垂直間隔がDBと一定な間隙部SBを設
けられている。間隙部SAにおいて凝集体Ｇが分離され、
反応液Ｌ中の検出に不要な液体媒体が間隙部SBに流れ
る。FIG. 15 shows a second modification of the sector sector 5, in which a gap SA in which the vertical interval is uniformly reduced from the maximum interval DA to the minimum interval DB, and a gap SB in which the vertical interval is fixed to DB. Is provided. Aggregate G is separated in gap SA,
A liquid medium unnecessary for detection in the reaction liquid L flows to the gap SB.
【００２６】[0026]
【発明の効果】以上説明したように本発明に係る検体測
定方法及び検体測定装置は、複数種の免疫学的活性物質
をそれぞれ担持させた粒子径又は及び光学的特性の異な
る担体粒子を用い、担体粒子の最大径よりも十分に大き
い最大間隔から担体粒子の最小径よりも小さい最小間隔
まで、徐々に減少した間隙を設けた簡素な試料台におい
て、この間隙の最大間隔の開口から反応液を注入する
と、間隔差によって大きさが異なる担体粒子、凝集体、
液体媒体等が分離できるので、反応液の凝集程度が明瞭
に判別、識別でき、予め作成した検量線と比較する等に
よって、検体中の複数種の免疫学的活性物質の定性的又
は定量的検出を１回の操作で高精度に行うことができ
る。As described above, the sample measuring method and the sample measuring apparatus according to the present invention use carrier particles having different particle diameters and optical characteristics each carrying a plurality of types of immunologically active substances, On a simple sample stage provided with a gradually reduced gap from a maximum interval sufficiently larger than the maximum diameter of the carrier particles to a minimum interval smaller than the minimum diameter of the carrier particles, the reaction solution is supplied from the opening at the maximum interval of the gap. When injected, carrier particles, agglomerates,
Since liquid medium can be separated, the degree of aggregation of the reaction solution can be clearly identified and identified, and qualitative or quantitative detection of multiple types of immunologically active substances in a sample can be performed by comparing with a previously prepared calibration curve. Can be performed with a single operation with high accuracy.
【図１】第１の実施例による試料台の斜視図である。FIG. 1 is a perspective view of a sample stage according to a first embodiment.
【図２】縦断面図である。FIG. 2 is a longitudinal sectional view.
【図３】凝集体の分離状態の説明図である。FIG. 3 is an explanatory diagram of a state of separation of aggregates.
【図４】Ａ−Ｂ方向を鉛直方向とした試料台の斜視図で
ある。FIG. 4 is a perspective view of a sample stage in which the AB direction is a vertical direction.
【図５】色調と粒子径の異なる担体粒子を用いたときの
凝集体の分離状態の説明図である。FIG. 5 is an explanatory diagram of a separated state of an aggregate when carrier particles having different color tones and particle diameters are used.
【図６】第２の実施例による試料台の斜視図である。FIG. 6 is a perspective view of a sample stage according to a second embodiment.
【図７】縦面図である。FIG. 7 is a vertical view.
【図８】Ａ−Ｂ方向を鉛直方向とした試料台の斜視図で
ある。FIG. 8 is a perspective view of a sample stage in which the AB direction is a vertical direction.
【図９】第３の実施例による試料台の平面図である。FIG. 9 is a plan view of a sample stage according to a third embodiment.
【図１０】セクタの斜視図である。FIG. 10 is a perspective view of a sector.
【図１１】セクタの半径方向の断面図である。FIG. 11 is a sectional view in the radial direction of a sector.
【図１２】試料台の断面図である。FIG. 12 is a sectional view of a sample stage.
【図１３】セクタの第１の変形例の断面図である。FIG. 13 is a sectional view of a first modification of the sector.
【図１４】凝集体の分離状態の説明図である。FIG. 14 is an explanatory diagram of a separated state of an aggregate.
【図１５】セクタの第２の変形例の断面図である。FIG. 15 is a sectional view of a second modification of the sector.
１ 試料台 ２ 基板 ３ カバー部材 ３ａ、３ｂ 凹部 ４ 基台 ４ａ、４ｂ 間隙部 ５ セクタ DESCRIPTION OF SYMBOLS 1 Sample stand 2 Substrate 3 Cover member 3a, 3b Depression 4 Base 4a, 4b Gap part 5 sector
フロントページの続き (72)発明者 田中 和實 東京都大田区下丸子三丁目30番２号 キ ヤノン株式会社内 (72)発明者 宮崎 健 東京都大田区下丸子三丁目30番２号 キ ヤノン株式会社内 (56)参考文献 特開 昭62−81567（ＪＰ，Ａ) 実開 昭58−84565（ＪＰ，Ｕ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 33/543 G01B 5/08 Continued on front page (72) Inventor Kazumi Tanaka 3-30-2 Shimomaruko, Ota-ku, Tokyo Canon Inc. (72) Inventor Ken Miyazaki 3-2-2 Shimomaruko, Ota-ku, Tokyo Canon Inc. (56) References JP-A-62-81567 (JP, A) JP-A-58-84565 (JP, U) (58) Fields investigated (Int. Cl. 7 , DB name) G01N 33/543 G01B 5 / 08
Claims (7)
体粒子と検体との反応液中における前記担体粒子の凝集
の程度により、検体中の前記特定物質の測定を行う検体
測定方法において、第１の特定物質と結合する第１の物
質を担持させた第１の担体粒子、第２の特定物質と結合
する第２の物質を担持させ前記第１の担体粒子とは異な
る種類の第２の担体粒子、検体を液体媒体中で反応させ
て反応液を得る第１の工程と、該反応液中の担体粒子が
その凝集径に応じた間隔を持った間隙位置に挟まれてト
ラップされるように最大間隔から間隔が徐々に減少する
間隙部に前記第１の工程で得られる反応液を注入する第
２の工程と、前記第１及び第２の担体粒子が前記間隙部
中で挟まれてトラップされる位置を基に検体中の前記第
１及び第２の特定物質をそれぞれ区別して測定する第３
の工程とを有することを特徴とする検体測定方法。1. A sample measuring method for measuring said specific substance in a sample according to the degree of agglomeration of said carrier particles in a reaction solution between a sample and carrier particles carrying a substance that binds to a specific substance, first carrier particles supporting the first agent that binds to one particular material, different types of second and said to carry a second substance which binds to a second specific substance first support particles Reaction of carrier particles and analyte in liquid medium
The first step of obtaining a reaction solution by the reaction, the carrier particles in the reaction solution
It is sandwiched between gaps with an interval corresponding to the aggregation diameter.
A second step of injecting the reaction solution obtained in the first step into the gap where the gap gradually decreases from the maximum gap so as to be wrapped , and wherein the first and second carrier particles are in the gap. A third method for measuring the first and second specific substances in the specimen separately based on the position where the first and second specific substances are interposed and trapped
And a method for measuring a sample.
なる請求項１に記載の検体測定方法。2. The sample measuring method according to claim 1, wherein the first and second carrier particles have different particle diameters.
異なる請求項１に記載の検体測定方法。3. The sample measuring method according to claim 1, wherein the first and second carrier particles have different optical characteristics.
光学特性が異なる請求項１に記載の検体測定方法。4. The sample measuring method according to claim 1, wherein the first and second carrier particles have different particle diameters and optical characteristics.
体粒子と検体との反応液中における前記担体粒子の凝集
の程度により、検体中の前記特定物質の測定を行う検体
測定装置において、複数の扇型セクタに分割した円板形
状を有し、これらの各セクタでは中心から外側に向かっ
て一様に又は段階的に減少した間隙部を有し、少なくと
も前記間隙部の最大間隔部及び最小間隔部の２端を開口
し、前記間隙部を囲む少なくとも１面が透明であり、最
大間隔が担体粒子の径よりも十分大きく、最小間隔が担
体粒子の径よりも小さいことを特徴とする検体測定装
置。5. A sample measuring apparatus for measuring the specific substance in a sample according to the degree of aggregation of the carrier particles in a reaction liquid between the sample and the carrier particles carrying a substance that binds to the specific substance, Each of these sectors has a gap that decreases uniformly or stepwise from the center to the outside, and at least a maximum gap and a minimum gap of the gap are provided. A specimen characterized in that two ends of the gap are opened, at least one surface surrounding the gap is transparent, the maximum gap is sufficiently larger than the diameter of the carrier particles, and the minimum gap is smaller than the diameter of the carrier particles. measuring device.
異なるようにした請求項５に記載の検体測定装置。6. The sample measuring apparatus according to claim 5, wherein a maximum interval and a minimum interval of each sector are different.
ようにした請求項５に記載の検体測定装置。7. The sample measuring apparatus according to claim 5, wherein the intervals between the sectors are different from each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4190063A JP3058766B2 (en) | 1992-06-24 | 1992-06-24 | Sample measuring method and sample measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4190063A JP3058766B2 (en) | 1992-06-24 | 1992-06-24 | Sample measuring method and sample measuring device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0611508A JPH0611508A (en) | 1994-01-21 |
| JP3058766B2 true JP3058766B2 (en) | 2000-07-04 |
Family
ID=16251733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4190063A Expired - Fee Related JP3058766B2 (en) | 1992-06-24 | 1992-06-24 | Sample measuring method and sample measuring device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3058766B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6242029B1 (en) | 1998-03-12 | 2001-06-05 | International Flavors & Fragrances Inc. | Food and beverage additive composition produced from saccharum officinarum leaves |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5156960B2 (en) * | 2008-02-21 | 2013-03-06 | 新潟県 | Method for separating and analyzing sample components using a microchemical analysis system |
| JP5189201B2 (en) * | 2008-04-02 | 2013-04-24 | アボット ポイント オブ ケア インコーポレイテッド | Virtual separation of bound and free labels in ligand assays to perform immunoassays of biological fluids containing whole blood |
-
1992
- 1992-06-24 JP JP4190063A patent/JP3058766B2/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6242029B1 (en) | 1998-03-12 | 2001-06-05 | International Flavors & Fragrances Inc. | Food and beverage additive composition produced from saccharum officinarum leaves |
| US6245376B1 (en) | 1998-03-12 | 2001-06-12 | International Flavors & Fragrances Inc. | Cola beverages comprising tastand additives from Saccharum officinarum leaves |
| US6251193B1 (en) | 1998-03-12 | 2001-06-26 | International Flavors & Fragrances Inc. | Use of spray-dried and freeze-dried sugarcane leaf essence |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0611508A (en) | 1994-01-21 |
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