JP2966057B2 - Automatic cell processing device - Google Patents
Automatic cell processing deviceInfo
- Publication number
- JP2966057B2 JP2966057B2 JP2189400A JP18940090A JP2966057B2 JP 2966057 B2 JP2966057 B2 JP 2966057B2 JP 2189400 A JP2189400 A JP 2189400A JP 18940090 A JP18940090 A JP 18940090A JP 2966057 B2 JP2966057 B2 JP 2966057B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- tube
- container
- nozzle
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000012545 processing Methods 0.000 title claims description 27
- 239000003153 chemical reaction reagent Substances 0.000 claims description 42
- 239000007788 liquid Substances 0.000 claims description 28
- 238000011068 loading method Methods 0.000 claims description 28
- 238000001914 filtration Methods 0.000 claims description 21
- 238000005406 washing Methods 0.000 claims description 8
- 210000004027 cell Anatomy 0.000 description 39
- 238000000684 flow cytometry Methods 0.000 description 16
- 239000002699 waste material Substances 0.000 description 13
- 239000002245 particle Substances 0.000 description 9
- 239000012530 fluid Substances 0.000 description 7
- 239000007850 fluorescent dye Substances 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 230000035484 reaction time Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000007781 pre-processing Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000007599 discharging Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】 （産業上の利用分野） この発明に係る自動細胞処理装置は、細胞の大きさや
相対的DNA量の測定を行なう為のフローサイトメトリー
を実施する為の前処理等を、自動的に行なう為に利用す
る。DETAILED DESCRIPTION OF THE INVENTION (Industrial application field) The automatic cell processing apparatus according to the present invention includes pretreatment for performing flow cytometry for measuring cell size and relative DNA amount, and the like. , To use automatically.
（従来の技術） 細胞生物学、細胞免疫学、癌の細胞診断学等の分野に
於いて、取り扱う細胞をその大きさや形態、細胞内物質
の含有量等の性格によって分類する為、フローサイトメ
トリーと呼ばれる測定方法を実施する場合がある。(Prior art) In the field of cell biology, cell immunology, cytodiagnosis of cancer, etc., flow cytometry is used to classify cells to be handled according to their size, morphology, and content of intracellular substances. May be implemented.
フローサイトメトリーは、蛍光色素で染色した細胞
を、１個ずつ遊離した状態で細い管の内側を通過させつ
つ、この細胞にレーザ光線を当てて蛍光を発生させ、こ
の蛍光の強弱を測定する事で、細胞の大きさや相対的DN
A量を測定するものである。In flow cytometry, cells stained with a fluorescent dye are allowed to pass through a thin tube in a state of being released one by one, and at the same time, the cells are irradiated with a laser beam to generate fluorescence, and the intensity of the fluorescence is measured. And cell size and relative DN
It measures A content.
この様なフローサイトメトリーによる測定作業を行な
う場合には、測定作業に先立って、測定すべき細胞を蛍
光色素で染色する等の、前処理作業を行なわなければな
らない。When performing such a measurement operation by flow cytometry, a pretreatment operation such as staining the cells to be measured with a fluorescent dye must be performed prior to the measurement operation.
この様な前処理作業は、例えば第７図に示す様な多く
の工程から成っており、この工程を決められた通りの順
番で行なわなければならない。Such a pretreatment operation comprises a number of steps as shown in FIG. 7, for example, and these steps must be performed in a predetermined order.
（発明が解決しようとする課題） 上述の様な複雑な行程を有する前処理作業を、従来は
人手により行なっていたが、作業完了迄に多くの時間を
要し、その間作業員が拘束されるだけでなく、作業員に
よって作業にバラツキが生じる為、省力化と処理の安定
化の為にも、自動化が望まれていた。(Problems to be Solved by the Invention) Conventionally, the pre-processing work having a complicated process as described above has been performed manually, but it takes a lot of time to complete the work, and the worker is restrained during that time. In addition, since the work varies depending on the worker, automation has been desired in order to save labor and stabilize processing.
ところが、前処理作業の行程を総て自動化する事は難
しく、従来は実用的な自動処理装置が知られていなかっ
た。However, it is difficult to automate all the steps of the pre-processing work, and no practical automatic processing apparatus has been hitherto known.
本発明の自動細胞処理装置は、この様な事情に鑑みて
考えられたものである。The automatic cell processing apparatus of the present invention has been conceived in view of such circumstances.
（課題を解決する為の手段） 本発明の自動細胞処理装置は、処理すべき細胞を含む
液状の検体を納めた検体管の上方に、この検体管に対す
る相対的な昇降を自在として設けられ、この検体管内へ
の液体の給排を自在とした出し入れノズルと、この出し
入れノズルに一端を連通自在とした出し入れ容器と、こ
の出し入れ容器と上記出し入れノズルとの間に直列に設
けられた、比較的目の粗い不適検体除去フィルタと、上
記出し入れ容器の他端に直列に接続された、比較的目の
細かい検体濾過フィルタと、一端を上記不適検体除去フ
ィルタと上記出し入れ容器との間に、他端を上記検体濾
過フィルタよりも上記出し入れ容器から離れた部分に、
それぞれ連通及び遮断自在に接続し、上記出し入れ容器
と検体濾過フィルタとを短絡自在なバイパスチューブ
と、上記検体濾過フィルタを介して上記出し入れ容器の
他端に連通自在な負圧ポンプと、この負圧ポンプにより
生じる負圧と大気圧との圧力差よりも大きな圧力差を大
気圧に対して生じさせ、上記負圧ポンプに代わって、上
記検体濾過フィルタを介して上記出し入れ容器の他端に
除去自在な加圧ポンプと、上記検体管内に試薬を滴下す
る為の滴下手段と、検体管内に存在する不要の液体を排
出する為の排液手段と、洗浄液を上記不適検体除去フィ
ルタと出し入れノズルとを通過させてから廃棄する事
で、不適検体除去フィルタに捕集された不適検体を除去
すると共に、上記不適検体除去フィルタと出し入れノズ
ルとを洗浄する洗浄手段とから構成されている。(Means for Solving the Problems) The automatic cell processing apparatus of the present invention is provided above a sample tube containing a liquid sample containing cells to be processed, so as to be freely movable up and down relative to the sample tube, An inlet / outlet nozzle capable of freely supplying / discharging a liquid into / from the sample tube, an inlet / outlet container having one end freely connected to the inlet / outlet nozzle, and a relatively provided in series between the inlet / outlet container and the inlet / outlet nozzle. A coarse unsuitable sample removal filter, a relatively fine sample filtration filter connected in series to the other end of the access container, one end between the unsuitable sample removal filter and the access container, and the other end In the part away from the access container than the sample filtration filter,
A bypass tube, which is connected so as to be able to freely communicate and shut off, and which can short-circuit the access container and the sample filtration filter, a negative pressure pump which can communicate with the other end of the access container via the sample filter, and A pressure difference greater than the pressure difference between the negative pressure generated by the pump and the atmospheric pressure is generated with respect to the atmospheric pressure, and can be removed to the other end of the access container via the sample filtration filter instead of the negative pressure pump. A pressurizing pump, a dropping unit for dropping a reagent into the sample tube, a draining unit for discharging unnecessary liquid existing in the sample tube, The washing means for removing the unsuitable sample collected by the unsuitable sample removal filter and discarding the unsuitable sample removal filter and the inlet / outlet nozzle by discarding after passing through. It is composed of a.
（作用） 上述の様に構成される本発明の自動細胞処理装置によ
り、フローサイトメトリーの為の前処理等、細胞の処理
作業を行なう場合、先ず滴下手段により、処理すべき細
胞を含む液状の検体を納めた検体管内に、蛍光色素等の
所定の試薬を滴下する。(Action) When the cell processing operation such as pretreatment for flow cytometry is performed by the automatic cell processing apparatus of the present invention configured as described above, first, the liquid containing the cell to be processed is dropped by the dropping means. A predetermined reagent such as a fluorescent dye is dropped into a sample tube containing a sample.
次いで検体管内に出し入れノズルを挿入し、負圧ポン
プと出し入れ容器の他端とを連通して、検体管内の液状
検体を出し入れノズルを通じ、出し入れ容器内に吸引す
る。Next, the insertion / exit nozzle is inserted into the sample tube, and the negative pressure pump is communicated with the other end of the insertion / extraction container, and the liquid sample in the sample tube is sucked into the insertion / extraction container through the insertion / exit nozzle.
この状態で出し入れ容器の他端に、負圧ポンプと加圧
ポンプとを交互に連通させる事により、この検体管内の
検体を、上記出し入れノズルに接続された出し入れ容器
に吸引したり、或は出し入れ容器内に吸引された検体
を、再び出し入れノズルを通じて、検体管に戻したりす
る事で、上記検体と試薬とを攪拌し、検体と試薬とを反
応させる。In this state, the negative pressure pump and the pressurizing pump are alternately connected to the other end of the loading / unloading container, so that the sample in the sample tube is sucked into or removed from the loading / unloading container connected to the loading / unloading nozzle. The sample aspirated into the container is returned to the sample tube again through the inlet / outlet nozzle, whereby the sample and the reagent are stirred, and the sample and the reagent are reacted.
次いで、試薬と反応した検体を出し入れ容器内に吸引
し、この出し入れ容器内に保持した状態で、出し入れノ
ズルを検体管から抜き出し、バイパスチューブを通じて
不適検体除去フィルタの後背側に洗浄液を、加圧ポンプ
で加圧しつつ送り込んで、この不適検体除去フィルタに
捕集されていた不適検体を廃液トレイ等に排出すると共
に、出し入れノズル内を洗浄する。Next, the sample that has reacted with the reagent is aspirated into the loading / unloading container, and with the sample held in the loading / unloading container, the loading / unloading nozzle is withdrawn from the sample tube. The unsuitable sample collected by the unsuitable sample removal filter is discharged to a waste liquid tray or the like, and the inside of the taking-out nozzle is washed.
その後、出し入れノズルを再び検体管に挿入した状態
で、加圧ポンプにより検体濾過フィルタの後背側を加圧
し、上記出し入れ容器内に保持されていた検体を、出し
入れノズルを介して検体管に戻す。Thereafter, the back side of the sample filtration filter is pressurized by the pressurizing pump in a state where the access nozzle is inserted again into the sample tube, and the sample held in the access container is returned to the sample tube via the access nozzle.
上述の様な前処理を施された検体を納めた検体管は、
自動細胞処理装置から取り出し、上記検体を、フローサ
イトメトリーを行なう装置に移し替える。The sample tube containing the sample that has been subjected to the pretreatment as described above is
The sample is taken out from the automatic cell processing apparatus, and the sample is transferred to an apparatus for performing flow cytometry.
（実施例） 次に、図示の実施例を説明しつつ、本発明を更に詳し
く説明する。(Example) Next, the present invention will be described in more detail while describing the illustrated example.
第１〜６図は本発明の自動細胞処理装置の回路構成を
示しており、第１図は基本構成を示す回路図、第２〜６
図は細胞処理作業を行程順に示す回路図である。1 to 6 show a circuit configuration of the automatic cell processing apparatus of the present invention. FIG. 1 is a circuit diagram showing a basic configuration, and FIGS.
The figure is a circuit diagram showing the cell processing operation in the order of steps.
１は試験管等の検体管で、この検体管１内には、処理
すべき細胞を含む液状の検体を納める。この検体管１の
上方には出し入れノズル２が、昇降及び水平方向の移動
自在に設けられている。但し、上記検体管１及び後述す
る廃液トレイ27（第４図）を移動自在とすれば、上記出
し入れノズル２を移動自在とする必要はない。Reference numeral 1 denotes a sample tube such as a test tube, in which a liquid sample containing cells to be treated is stored. Above the sample tube 1, a take-in / out nozzle 2 is provided so as to be movable up and down and horizontally. However, if the sample tube 1 and a waste liquid tray 27 (FIG. 4), which will be described later, are movable, it is not necessary to make the loading / unloading nozzle 2 movable.
上記出し入れノズル２には、液状の検体を一時保持し
ておく為の出し入れ容器３の一端を連通自在としてい
る。又、この出し入れ容器３と上記出し入れノズル２と
の間には、上記出し入れノズル２の側から順に、比較的
目の粗い（例えば40μｍ以上の粒子を捕集する。）不適
検体除去フィルタと第一の三方弁５とを、互いに直列に
設けている。One end of a loading / unloading container 3 for temporarily holding a liquid sample is allowed to communicate with the loading / unloading nozzle 2. In addition, between the loading / unloading container 3 and the loading / unloading nozzle 2, in order from the loading / unloading nozzle 2 side, a relatively coarse (for example, to collect particles of 40 μm or more) unsuitable sample removing filter and a first filter. And the three-way valve 5 are provided in series with each other.
上記出し入れ容器３の他端にその一端を接続した、給
排チューブ22の途中には、比較的目の細かい（例えば１
μｍ以上の粒子を捕集する。）検体濾過フィルタ６と第
二の三方弁７とを、上記出し入れ容器３の側から順に、
互いに直列に設けている。そして、上記給排チューブ22
の他端を、第三の三方弁14に接続している。In the middle of the supply / discharge tube 22, one end of which is connected to the other end of the access container 3, relatively fine eyes (for example, 1
Collect particles of μm or more. ) The sample filtration filter 6 and the second three-way valve 7 are sequentially placed from the access container 3 side.
They are provided in series with each other. Then, the supply / discharge tube 22
Is connected to the third three-way valve 14.
上記第一、第二の三方弁５、７の残りのポートには、
それぞれバイパスチューブ８の両端部を接続し、このバ
イパスチューブ８によって、上記出し入れ容器３と検体
濾過フィルタ６とを短絡自在としている。In the remaining ports of the first and second three-way valves 5 and 7,
Both ends of the bypass tube 8 are connected to each other, and the bypass tube 8 allows the access container 3 and the sample filtration filter 6 to be short-circuited freely.
９は、上記検体濾過フィルタ６を介して上記出し入れ
容器３の他端に連通自在な負圧ポンプ、10は、同じく上
記出し入れ容器３の他端に連通自在な加圧ポンプであ
る。この加圧ポンプ10が発生する加圧力は、例えば＋38
0mm/Hg程度と、上記負圧ポンプ９が発生する、例えば−
200mm/Hg程度の負圧力よりも、十分に大きな絶対値を持
っている。尚、負圧ポンプ９と加圧ポンプ10とは、単一
のコンプレッサの吸入口と吐出口とする事も出来る。但
し、この場合には、上記吸入口に負圧調整弁11を、吐出
口に加圧調整弁12を、それぞれ設けて、負圧力と加圧力
とが、上記した条件を満たす様にする。Reference numeral 9 denotes a negative pressure pump that can communicate with the other end of the access container 3 via the sample filtration filter 6, and reference numeral 10 denotes a pressure pump that can also communicate with the other end of the access container 3. The pressure generated by the pressure pump 10 is, for example, +38
When the negative pressure pump 9 is generated at about 0 mm / Hg, for example, −
It has an absolute value sufficiently larger than the negative pressure of about 200 mm / Hg. It should be noted that the negative pressure pump 9 and the pressure pump 10 can be used as a suction port and a discharge port of a single compressor. However, in this case, a negative pressure adjusting valve 11 is provided at the suction port, and a pressure adjusting valve 12 is provided at the discharge port, so that the negative pressure and the pressing force satisfy the above conditions.
上記負圧ポンプ９と加圧ポンプ10との内、負圧ポンプ
９の吸入口に設けた負圧調整弁11と、前記第三の三方弁
14とは、吸引チューブ13により連通している。A negative pressure regulating valve 11 provided at the suction port of the negative pressure pump 9 among the negative pressure pump 9 and the pressure pump 10;
14 is in communication with the suction tube 13.
又、上記加圧ポンプ10の吐出口に設けた加圧調整弁12
と上記第三の三方弁14とは、加圧チューブ15によって連
通自在とし、この加圧チューブ15の途中に、第一の開閉
弁16を設けている。A pressure adjusting valve 12 provided at the discharge port of the pressure pump 10
The third three-way valve 14 and the third three-way valve 14 can communicate with each other by a pressurizing tube 15, and a first on-off valve 16 is provided in the middle of the pressurizing tube 15.
又、上記加圧チューブ15の途中で、加圧調整弁12と第
一の開閉弁16との間位置からその一端を分岐した、分岐
加圧チューブ17の他端を、試薬ボトル18の上部空間に開
口させている。一方、この試薬ボトル18の底部にその一
端を開口させた、送り出しチューブ19の途中に、ロータ
リ式切換弁20、第二の開閉弁21を接続している。この内
のロータリ式切換弁20は、前記検体管１に複数種類の試
薬を滴下する必要がある場合に、これを選択する為に利
用するものである。そして、上記送り出しチューブ19の
他端は、前記加圧チューブ15の途中で、前記第一の開閉
弁16と第三の三方弁14との間位置に接続している。尚、
複数種類の試薬を貯溜する為の、複数の試薬ボトル18の
上部空間には、それぞれ分岐加圧チューブ17の他端を連
通させておく。In the middle of the pressurizing tube 15, one end thereof is branched from a position between the pressurizing regulating valve 12 and the first on-off valve 16, and the other end of the branch pressurizing tube 17 is connected to the upper space of the reagent bottle 18. It has an opening. On the other hand, a rotary switching valve 20 and a second on-off valve 21 are connected in the middle of the delivery tube 19 having one end opened at the bottom of the reagent bottle 18. The rotary switching valve 20 is used to select a plurality of types of reagents when it is necessary to drop them on the sample tube 1. The other end of the delivery tube 19 is connected to a position between the first opening / closing valve 16 and the third three-way valve 14 in the middle of the pressure tube 15. still,
The other ends of the branch pressurizing tubes 17 are respectively connected to upper spaces of the plurality of reagent bottles 18 for storing a plurality of types of reagents.
更に、前記給排チューブ22の途中で、前記第二、第三
の三方弁７、14の間位置にその一端を接続し、途中に第
三の開閉弁25を設けた廃液チューブ23の他端は、排液ボ
トル24内に開口させている。そして、この廃液ボトル24
の上部にその一端を開口した排気チューブ26の他端を、
前記負圧調整弁11を介して、前記負圧ポンプ９の吸入口
に通じている。Further, in the middle of the supply / discharge tube 22, one end thereof is connected between the second and third three-way valves 7 and 14, and the other end of the waste liquid tube 23 provided with a third opening / closing valve 25 in the middle. Are opened in the drainage bottle 24. And this waste bottle 24
The other end of the exhaust tube 26, one end of which is open at the top of the
It communicates with the suction port of the negative pressure pump 9 through the negative pressure regulating valve 11.
上述の様に構成される本発明の自動細胞処理装置によ
り、フローサイトメトリーの為の前処理等、細胞の処理
作業を行なう場合、先ず第２図に太い実線で示す系統を
通じて流体が流れる様に、各弁を切り換え、加圧ポンプ
10を運転する。この結果、試薬ボトル18内の試薬が加圧
され、この試薬が、送り出しチューブ19、ロータリ式切
換弁20、第二の開閉弁21、加圧チューブ15、給排チュー
ブ22、第二の三方弁７、検体濾過フィルタ６、出し入れ
容器３、第一の三方弁５、不適検体除去フィルタ４を通
じて出し入れノズル２に送られ、この出し入れノズル２
から、検体管１内に滴下される。滴下を中止する際に
は、それ迄開いていた第二の開閉弁21を閉じる。When the cell processing operation such as pretreatment for flow cytometry is performed by the automatic cell processing apparatus of the present invention configured as described above, first, the fluid is caused to flow through the system shown by the thick solid line in FIG. , Switching each valve, pressurizing pump
Driving 10 As a result, the reagent in the reagent bottle 18 is pressurized, and the reagent is supplied to the delivery tube 19, the rotary switching valve 20, the second opening / closing valve 21, the pressurizing tube 15, the supply / discharge tube 22, and the second three-way valve. 7, the sample filtration filter 6, the access container 3, the first three-way valve 5, and the inappropriate sample removal filter 4 are sent to the access nozzle 2.
From the sample tube 1. When the dripping is stopped, the second on-off valve 21 that has been opened is closed.
上述の様にして、処理すべき細胞を含む液状の検体を
納めた検体管１内に、蛍光色素等の所定の試薬を滴下し
たならば、次いで出し入れノズル２を下降させる（又は
検体管１を上昇させる）事により、検体管１の底部に迄
出し入れノズル２を挿入する。そしてこの状態のまま、
第３図に太い実線で示す系統を通じて流体が流れる様
に、各弁を切り換え、負圧ポンプ９を運転する。この結
果、出し入れ容器３内の圧力が低下し、検体管１内の液
状の検体が、出し入れノズル２、不適検体除去フィルタ
４、第一の三方弁５を通じて、上記出し入れ容器３内に
吸引される。所定時間経過する事で、検体管１内の検体
の全部又は一部が、出し入れ容器３に移されたならば、
出し入れノズル２の下端を検体管１の底部に迄挿入した
状態のまま、第３図に太い波線で示す系統を通じて流体
が流れる様に、第三の三方弁14を切り換え、加圧ポンプ
10を運転する。この結果、出し入れ容器３内の圧力が上
昇し、出し入れ容器３内の液状の検体が、第一の三方弁
５、不適検体除去フィルタ４、出し入れノズル２を通じ
て、検体管１内に戻される。この様に、検体管１と出し
入れ容器３との間で、試薬を滴下された検体のやり取り
を行なう事により、上記試薬と検体とを攪拌混合し、検
体と試薬とを反応させる。この作業は、前記第７図に示
す様な作業を行なうべく、異なる試薬毎に必要に応じて
繰り返し行なう。尚、出し入れ容器３部分には、必要に
応じて加温、冷却機構を付設し、この出し入れ容器３内
に送り込まれた検体と試薬との反応が、一定条件で行な
われる様にする。As described above, when a predetermined reagent such as a fluorescent dye is dropped into the sample tube 1 containing the liquid sample containing the cells to be treated, the access nozzle 2 is then lowered (or the sample tube 1 is moved down). The nozzle 2 is inserted to the bottom of the sample tube 1. And in this state,
The valves are switched and the negative pressure pump 9 is operated so that the fluid flows through the system shown by the thick solid line in FIG. As a result, the pressure in the loading / unloading container 3 decreases, and the liquid sample in the sample tube 1 is sucked into the loading / unloading container 3 through the loading / unloading nozzle 2, the unsuitable sample removing filter 4, and the first three-way valve 5. . If a predetermined time has elapsed and all or a part of the sample in the sample tube 1 has been transferred to the access container 3,
With the lower end of the access nozzle 2 inserted to the bottom of the sample tube 1, the third three-way valve 14 is switched so that the fluid flows through the system shown by the thick wavy line in FIG.
Driving 10 As a result, the pressure in the loading / unloading container 3 increases, and the liquid sample in the loading / unloading container 3 is returned to the sample tube 1 through the first three-way valve 5, the inappropriate sample removing filter 4, and the loading / unloading nozzle 2. In this manner, by exchanging the sample in which the reagent has been dropped between the sample tube 1 and the loading / unloading container 3, the reagent and the sample are stirred and mixed, and the sample and the reagent are reacted. This operation is repeated as necessary for each different reagent in order to perform the operation shown in FIG. In addition, a heating and cooling mechanism is added to the portion of the loading / unloading container 3 as necessary, so that the reaction between the sample sent into the loading / unloading container 3 and the reagent is performed under constant conditions.
又、検体管１と出し入れ容器３との間で検体及び試薬
のやり取りを行なう事で、検体と試薬とが攪拌混合され
るだけでなく、上記検体中に含まれる細胞塊を分散させ
る事も出来る。即ち、上記検体中には、フローサイトメ
トリーの実施に適さない程大きな細胞塊が存在するが、
この細胞塊は、上記検体管１と出し入れ容器３との間に
設けた不適検体除去フィルタ４を通過する事でほぐさ
れ、個々の細胞に分散される。この様な細胞の分散をよ
り効率的に行なう為、検体管１又は不適検体除去フィル
タ４を超音波振動させる加振機構を設けたり、或は不適
検体除去フィルタ４を、目の大きさの異なる複数のフィ
ルタを互いに直列に配置することにより構成しても良
い。即ち、検体管１の側から順に、例えば1mm迄の塊を
通過させるフィルタと200μｍ迄の塊を通過させるフィ
ルタと40μｍ迄の塊を通過させるフィルタとを直列に配
置すれば、大きな塊を効率良くほぐし、不適検体除去フ
ィルタ４に過度の目詰りが生じない様に出来る。Further, by exchanging the sample and the reagent between the sample tube 1 and the loading / unloading container 3, not only the sample and the reagent are stirred and mixed, but also the cell mass contained in the sample can be dispersed. . That is, in the sample, there is a cell mass large enough to be unsuitable for performing flow cytometry,
The cell mass is loosened by passing through an inappropriate sample removing filter 4 provided between the sample tube 1 and the loading / unloading container 3, and dispersed into individual cells. In order to perform such cell dispersion more efficiently, a vibrating mechanism for ultrasonically oscillating the sample tube 1 or the unsuitable sample removing filter 4 is provided, or the unsuitable sample removing filter 4 is provided with different eye sizes. You may comprise by arrange | positioning several filters mutually in series. That is, if a filter that passes a lump of up to 1 mm, a filter that passes a lump of up to 200 μm, and a filter that passes a lump of up to 40 μm are arranged in series from the sample tube 1 side in order, a large lump can be efficiently removed. It is possible to prevent the clogging of the unsuitable sample removing filter 4 from being excessively clogged.
又、上述の様にして出し入れ容器３内で検体と試薬と
を反応させる途中で、或は反応終了時に出し入れ容器３
内に検体を保持したまま、出し入れノズル２を検体管１
から抜き出して、この出し入れノズル２を廃液トレイ27
上に移動させてから、第４図に太い実線で示す系統を通
じて流体が流れる様に、各弁を切り換えると共に、ロー
タリ式切換弁20の切り換えによって上記系統中に、洗浄
液を貯溜した試薬ボトル18aを接続して、加圧ポンプを
運転する。Also, as described above, during the reaction between the sample and the reagent in the loading / unloading container 3, or at the end of the reaction, the loading / unloading container 3
With the sample held inside, the insertion / removal nozzle 2 is connected to the sample tube 1
From the waste liquid tray 27.
After being moved upward, the valves are switched so that the fluid flows through the system shown by the thick solid line in FIG. 4, and the reagent bottle 18a storing the washing liquid is stored in the system by switching the rotary switching valve 20. Connect and operate the pressurized pump.
この結果、上記試薬ボトル18a内の洗浄液が、送り出
しチューブ19、ロータリ式切換弁20、第二の開閉弁21、
加圧チューブ15、第三の三方弁14、給排チューブ22、第
二の三方弁７、バイパスチューブ８、第一の三方弁５を
介して、不適検体除去フィルタ４の後背側に送り込まれ
る。As a result, the washing liquid in the reagent bottle 18a is supplied by the delivery tube 19, the rotary switching valve 20, the second on-off valve 21,
The filter is sent to the rear of the inappropriate sample removing filter 4 via the pressurizing tube 15, the third three-way valve 14, the supply / discharge tube 22, the second three-way valve 7, the bypass tube 8, and the first three-way valve 5.
加圧ポンプ10から吐出される圧縮空気の圧力に基づ
き、不適検体除去フィルタ４の後背側に送り込まれる洗
浄液の圧力は、負圧ポンプ９の運転に基づいて得られ
る、上記不適検体除去フィルタ４の全面に不適検体を捕
集する為の圧力よりも十分に大きい為、上記洗浄液の送
り込みに伴なって不適検体除去フィルタ４の前面に捕集
されていた比較的大きな粒子（不適検体）が、洗浄液と
共に廃液トレイ27を通じ廃液ボトル24に排出されて、検
体中から不適検体が除去されると共に、不適検体除去フ
ィルタ４及び出し入れノズル２内が奇麗な状態となる。Based on the pressure of the compressed air discharged from the pressurizing pump 10, the pressure of the cleaning liquid sent to the rear side of the inappropriate sample removing filter 4 is obtained based on the operation of the negative pressure pump 9. Since the pressure is sufficiently higher than the pressure for collecting the unsuitable sample on the entire surface, relatively large particles (unsuitable sample) collected on the front surface of the unsuitable sample removing filter 4 due to the feeding of the washing solution are removed. At the same time, the waste liquid is discharged to the waste liquid bottle 24 through the waste liquid tray 27, whereby the unsuitable sample is removed from the sample, and the inside of the unsuitable sample removal filter 4 and the inlet / outlet nozzle 2 is in a beautiful state.
上述の操作を行なって、検体と各試薬との反応が十分
に行なわれ、且つ不適検体の除去が行なわれたならば、
第５図に太い実線で示す様に流体が流れる様に各弁を切
り換え、出し入れ容器３内の検体を検体管１内に戻す。Performing the above operation, if the reaction between the sample and each reagent is sufficiently performed, and if the unsuitable sample is removed,
Each valve is switched so that the fluid flows as indicated by the thick solid line in FIG. 5, and the sample in the access container 3 is returned to the sample tube 1.
この場合に於いて、前述した反応に伴なって凝集した
細胞が、不適検体除去フィルタ４を詰まらせ、出し入れ
容器３から検体管１に検体を戻せなくなる事が考えられ
る。そこで、この様な場合には、第５図に鎖線で示す様
に、途中に吐出弁28を有し、上記不適検体除去フィルタ
４をバイパスさせる吐出管29を介して、出し入れ容器３
から検体管１に検体を戻す様に構成する事も出来る。
尚、上記吐出管29の上流側端部は、第一の三方弁５より
も出し入れ容器３側に接続する事も出来る。In this case, it is conceivable that the cells aggregated by the above-mentioned reaction clog the inappropriate sample removing filter 4 and the sample cannot be returned from the access container 3 to the sample tube 1. In such a case, as shown by the dashed line in FIG. 5, a discharge valve 28 is provided in the middle, and a discharge pipe 29 for bypassing the unsuitable sample removing filter 4 is used.
From the sample tube 1 to the sample tube 1 can be configured.
Note that the upstream end of the discharge pipe 29 can be connected to the container 3 side with respect to the first three-way valve 5.
検体を検体管１内に戻した後、出し入れノズル２の下
端部を検体管１の底部に迄挿入した状態にして、第６図
に太い実線で示す様に、出し入れ容器３の他端を廃液ボ
トル24を介して、負圧ポンプ９の吸入口に通じさせる状
態に、各弁を切り換え、負圧ポンプ９を運転する。この
結果、廃液ボトル24並びに出し入れ容器３内の圧力が低
下し、検体管１内の検体が、同図に太い破線で示す経路
を通じて、出し入れ容器３内に吸引され、更にその内の
液状分が同図に太い実線で示す経路を通じて、廃液ボト
ル24に移され、この廃液ボトル24に溜められる。尚、こ
の作業は、検体を検体管１内に戻す事なく（第５図の状
態を省略し）、出し入れ容器３内に入れた状態から直ち
に行なっても良い。After the sample is returned into the sample tube 1, the lower end of the inlet / outlet nozzle 2 is inserted to the bottom of the sample tube 1, and the other end of the inlet / outlet container 3 is drained as shown by a thick solid line in FIG. The valves are switched to a state in which they are connected to the suction port of the negative pressure pump 9 via the bottle 24, and the negative pressure pump 9 is operated. As a result, the pressures in the waste liquid bottle 24 and the access container 3 are reduced, and the sample in the sample tube 1 is sucked into the access container 3 through a path shown by a thick broken line in FIG. The liquid is transferred to the waste liquid bottle 24 through the path indicated by the thick solid line in FIG. Note that this operation may be performed immediately after the sample is put in the access container 3 without returning the sample into the sample tube 1 (the state of FIG. 5 is omitted).
この様に検体管１内の検体の液状分が出し入れ容器３
を通じて廃液ボトル24に移される過程で、検体中に含ま
れる比較的大きな粒子が未だ残っている場合は、この大
きな粒子は不適検体除去フィルタ４に捕集され、比較的
小さな粒子で、フローサイトメトリーに使用するもの
は、検体濾過フィルタ６に捕集される。Thus, the liquid portion of the sample in the sample tube 1 is taken in and out of the container 3.
When relatively large particles contained in the sample still remain in the process of being transferred to the waste liquid bottle 24 through the process, the large particles are collected by the unsuitable sample removing filter 4 and are relatively small particles, which are used for flow cytometry. Are collected by the sample filtration filter 6.
この様に、比較的小さな検体粒子を検体濾過フィルタ
６に捕集したならば、再び第２図に太い実線で示す系統
を通じて流体が流れる様に、各弁を切り換え、加圧ポン
プ10を運転する。但し、試薬ボトル18は、前述の場合と
別のものとなる様に、ロータリ式切換弁20を切り換えて
おく。この結果、試薬ボトル18内の試薬が加圧され、こ
の試薬が、送り出しチューブ19、ロータリ式切換弁20、
第二の開閉弁21、加圧チューブ15、給排チューブ22、第
二の三方弁７を通じて検体濾過フィルタ６の後背側（第
２図の上側）に送られ、この検体濾過フィルタ６に捕集
されていた検体粒子を押し流しつつ、出し入れ容器３、
第一の三方弁５、不適検体除去フィルタ４を通じて出し
入れノズル２に送られ、この出し入れノズル２から検体
ごと、検体管１内に滴下される。滴下を中止する際に
は、それ迄開いていた第二の開閉弁21を閉じる。When the relatively small sample particles are collected in the sample filtration filter 6 in this manner, the valves are switched and the pressure pump 10 is operated so that the fluid flows again through the system shown by the thick solid line in FIG. . However, the rotary switching valve 20 is switched so that the reagent bottle 18 is different from the case described above. As a result, the reagent in the reagent bottle 18 is pressurized, and the reagent is supplied to the delivery tube 19, the rotary switching valve 20,
The sample is sent to the rear side (upper side in FIG. 2) of the sample filtration filter 6 through the second on-off valve 21, the pressure tube 15, the supply / discharge tube 22, and the second three-way valve 7, and is collected by the sample filtration filter 6. While flushing the sample particles that have been removed,
The sample is sent to the inlet / outlet nozzle 2 through the first three-way valve 5 and the inappropriate sample removing filter 4, and the sample is dropped from the outlet / outlet nozzle 2 into the sample tube 1 together with the sample. When the dripping is stopped, the second on-off valve 21 that has been opened is closed.
尚、この場合に於いても、前述した様に反応に伴なっ
て凝集した細胞が不適検体除去フィルタ４を詰まらせて
いた場合には、前記第５図に鎖線で示した吐出管29を介
して、試薬及び検体を滴下する。Also in this case, as described above, when the cells aggregated due to the reaction clog the unsuitable sample removing filter 4, the cells pass through the discharge pipe 29 shown by a chain line in FIG. Then, the reagent and the sample are dropped.
上述の様にして、検体濾過フィルタ６に捕集されてい
た検体粒子と、蛍光色素等、次の反応に使用する試薬を
検体管１内に戻したならば、前述の作業を繰り返しつ
つ、前記第７図に示す様な処理作業を続ける。As described above, when the sample particles collected in the sample filtration filter 6 and the reagent used for the next reaction, such as a fluorescent dye, are returned into the sample tube 1, the above-described operation is repeated, The processing operation shown in FIG. 7 is continued.
最後の反応が終了したならば、出し入れノズル２を検
体管１内に挿入した状態で、再び第５図に太い実線で示
す系統を通じて流体が流れる様に、各弁を切り換えて、
加圧ポンプ10を運転する。When the last reaction is completed, each valve is switched so that the fluid flows again through the system shown by the thick solid line in FIG.
The pressure pump 10 is operated.
この結果、加圧チューブ15、給排チューブ22を介して
検体濾過フィルタ６の後背側に圧力が作用し、検体が出
し入れ容器３、不適検体除去フィルタ４、出し入れノズ
ル２を介して、検体管１に戻される。As a result, a pressure acts on the rear side of the sample filtration filter 6 via the pressurizing tube 15 and the supply / discharge tube 22, and the sample passes through the container 3, the unsuitable sample removal filter 4, and the inlet / outlet nozzle 2 and the sample tube 1. Is returned to.
以上に述べた行程を順番に行なう事によって、所定の
前処理を施された検体を納めた検体管１は、自動細胞処
理装置から取り出し、上記検体を、フローサイトメトリ
ーを行なう装置に移し替える。但し、本発明の自動細胞
処理装置を構成する検体管１内に処理すべき検体を注入
したり、或は処理された検体をフローサイトメトリーを
行なう装置に移し替えたりする作業を自動化する事も出
来る。By sequentially performing the above-described steps, the sample tube 1 containing the sample that has been subjected to the predetermined pretreatment is removed from the automatic cell processing apparatus, and the sample is transferred to an apparatus that performs flow cytometry. However, it is also possible to automate the work of injecting the sample to be processed into the sample tube 1 constituting the automatic cell processing apparatus of the present invention, or transferring the processed sample to an apparatus for performing flow cytometry. I can do it.
例えば、バイパスチューブ８に一端を接続した送りチ
ューブの他端をフローサイトメトリーを行なう装置に接
続し、この送りチューブを通じて、上述した一連の前処
理を完了した検体を、上記フローサイトメトリーを行な
う装置に送り込む様に構成すれば、検体の移し替え作業
が容易となる。For example, the other end of the feed tube having one end connected to the bypass tube 8 is connected to an apparatus for performing flow cytometry, and the sample that has been subjected to the above-described series of pretreatments is subjected to the above-described apparatus for performing flow cytometry through the feed tube. If the configuration is such that the sample is transferred to the sample, the sample transfer operation becomes easy.
尚、自動細胞処理装置に複数本の検体管１をセット
し、この検体管１内への式薬の滴下作業や攪拌作業を、
１本の出し入れノズル２で行なった場合、検体管１によ
って滴下時期や攪拌時期がずれる事に伴ない、各検体管
１毎に反応時間の相違が生じるが、反応時間を厳密に規
制する必要がある場合には、検体管１の数に合わせて、
出し入れノズル２を複数個設ければ、上記複数の検体管
１への滴下作業等を同時に行なって、各検体管１毎に反
応時間がずれる事を防止出来る。又、反応温度を規制す
る為、恒温槽を設置する等の適宜手段により、検体管１
や出し入れノズル２、出し入れ容器３の温度制御を行な
う。In addition, a plurality of sample tubes 1 are set in the automatic cell processing apparatus, and a dropping operation and a stirring operation of the formula medicine into the sample tubes 1 are performed.
In the case of performing with one inlet / outlet nozzle 2, the reaction time differs for each sample tube 1 due to the shift of the dropping time and the stirring time depending on the sample tube 1, but it is necessary to strictly control the reaction time. In some cases, according to the number of sample tubes 1,
If a plurality of access nozzles 2 are provided, the dropping operation and the like onto the plurality of sample tubes 1 can be performed at the same time, and the reaction time can be prevented from being shifted for each sample tube 1. In addition, in order to regulate the reaction temperature, the sample tube 1 may be provided by an appropriate means such as installing a thermostat.
The temperature of the nozzle 2 and the container 3 is controlled.
更に、各検体管１内に滴下する試薬の中には、４℃程
度と、比較的低温で貯溜する必要のあるものが存在する
が、この様に低温で貯蔵された試薬を、そのまま検体管
１内に滴下した場合、温度が低い事により、反応時間が
長くなり過ぎる場合がある。Further, some of the reagents dropped into each sample tube 1 need to be stored at a relatively low temperature of about 4 ° C., and the reagent stored at such a low temperature is directly transferred to the sample tube. When dropped into 1, the reaction time may be too long due to the low temperature.
そこで、この様な場合には、試薬を低温で貯蔵した貯
蔵容器（図示せず）と出し入れノズル２とを結ぶ配管の
途中に、次回の滴下に使用する程度の、少量の試薬を貯
溜自在な一時貯溜部（図示せず）を、上記配管と直列に
設ければ、貯蔵容器から取り出された試薬が、上記一時
貯溜部に存在する間に、反応温度にしてから上記出し入
れノズル２に送られる様になり、反応時間の長期化を防
止出来る。Therefore, in such a case, a small amount of reagent that can be used for the next dropping can be freely stored in the middle of the pipe connecting the storage container (not shown) storing the reagent at a low temperature and the loading / unloading nozzle 2. If a temporary storage unit (not shown) is provided in series with the pipe, the reagent taken out of the storage container is brought to the reaction temperature and sent to the loading / unloading nozzle 2 while present in the temporary storage unit. The reaction time can be prevented from being prolonged.
（発明の効果） 本発明の自動細胞処理装置は、以上に述べた通り構成
され作用する為、複雑なフローサイトメトリーの前処理
作業等を自動的に行なう事が出来、省力化を図れると同
時に、常に安定した処理作業を行なう事が出来る為、前
処理後に行なうフローサイトメトリー等による病理診断
等の信頼性が向上する。(Effects of the Invention) Since the automatic cell processing apparatus of the present invention is configured and operates as described above, it is possible to automatically perform complicated flow cytometry pretreatment operations and the like, thereby saving labor. Since a stable processing operation can always be performed, the reliability of pathological diagnosis and the like by flow cytometry performed after preprocessing is improved.
尚、本発明の自動細胞処理装置は、フィルタの粗さを
調節する事で、血液中の赤血球を除去する等、フローサ
イトメトリー以外の細胞処理にも利用出来る。The automatic cell processing apparatus of the present invention can be used for cell processing other than flow cytometry, such as removing red blood cells in blood by adjusting the roughness of a filter.
第１〜６図は本発明の自動細胞処理装置の回路構成を示
しており、第１図は基本構成を示す回路図、第２〜６図
は細胞処理作業を行程順に示す回路図、第７図は本発明
の自動細胞処理装置により行なわれる前処理作業の１例
を示すフローチャートである。 1:検体管、2:出し入れノズル、3:出し入れ容器、4:不適
検体除去フィルタ、5:第一の三方弁、6:検体濾過フィル
タ、7:第二の三方弁、8:バイパスチューブ、9:負圧ポン
プ、10:加圧ポンプ、11:負圧調整弁、12:加圧調整弁、1
3:吸引チューブ、14:第三の三方弁、15:加圧チューブ、
16:第一の開閉弁、17:分岐加圧チューブ、18、18a:試薬
ボトル、19:送り出しチューブ、20:ロータリ式切換弁、
21:第二の開閉弁、22:給排チューブ、23:排液チュー
ブ、24:廃液ボトル、25:第三の開閉弁、26:排気チュー
ブ、27:廃液トレイ、28:吐出弁、29:吐出管。1 to 6 show a circuit configuration of the automatic cell processing apparatus of the present invention, FIG. 1 is a circuit diagram showing a basic configuration, FIGS. 2 to 6 are circuit diagrams showing a cell processing operation in the order of steps, FIG. The figure is a flowchart showing an example of a pre-processing operation performed by the automatic cell processing device of the present invention. 1: sample tube, 2: access nozzle, 3: access container, 4: unsuitable sample removal filter, 5: first three-way valve, 6: sample filtration filter, 7: second three-way valve, 8: bypass tube, 9 : Negative pressure pump, 10: Pressure pump, 11: Negative pressure control valve, 12: Pressure control valve, 1
3: suction tube, 14: third three-way valve, 15: pressurized tube,
16: first open / close valve, 17: branch pressurized tube, 18, 18a: reagent bottle, 19: delivery tube, 20: rotary switching valve,
21: second open / close valve, 22: supply / drain tube, 23: drain tube, 24: waste bottle, 25: third open / close valve, 26: exhaust tube, 27: waste tray, 28: discharge valve, 29: Discharge pipe.
Claims (3)
検体管の上方に、この検体管に対する相対的な昇降を自
在として設けられ、この検体管内への液体の給排を自在
とした出し入れノズルと、この出し入れノズルに一端を
連通自在とした出し入れ容器と、この出し入れ容器と上
記出し入れノズルとの間に直列に設けられた、比較的目
の粗い不適検体除去フィルタと、上記出し入れ容器の他
端に直列に接続された、比較的目の細かい検体濾過フィ
ルタと、一端を上記不適検体除去フィルタと上記出し入
れ容器との間に、他端を上記検体濾過フィルタよりも上
記出し入れ容器から離れた部分に、それぞれ連通及び遮
断自在に接続して、上記出し入れ容器と検体濾過フィル
タとを短絡自在なバイパスチューブと、上記検体濾過フ
ィルタを介して上記出し入れ容器の他端に接続自在な負
圧ポンプと、この負圧ポンプにより生じる負圧と大気圧
との圧力差よりも大きな圧力差を大気圧に対して生じさ
せ、上記負圧ポンプに代わって、上記検体濾過フィルタ
を介して上記出し入れ容器の他端に接続自在な加圧ポン
プと、上記検体管内に試薬を滴下する為の滴下手段と、
検体管内に存在する不要の液体を排出する為の排液手段
と、洗浄液を上記不適検体除去フィルタと出し入れノズ
ルとを通過させてから廃棄する事で、不適検体除去フィ
ルタに捕集された不適検体を除去すると共に、上記不適
検体除去フィルタと出し入れノズルとを洗浄する洗浄手
段とから成る自動細胞処理装置。1. A sample tube containing a liquid sample containing cells to be treated is provided above and below the sample tube so that the sample tube can be moved up and down relative to the sample tube. An in / out nozzle, an in / out container whose one end can freely communicate with the in / out nozzle, and a relatively coarse unsuitable sample removing filter provided in series between the in / out container and the in / out nozzle, and the in / out container The other end is connected in series, the relatively fine sample filtration filter, one end between the unsuitable sample removal filter and the access container, the other end farther from the access container than the sample filtration filter Parts are connected to each other so as to be able to communicate and shut off, and the access container and the sample filtration filter can be short-circuited freely via the bypass tube and the sample filtration filter. A negative pressure pump that can be freely connected to the other end of the access container, and a pressure difference greater than the pressure difference between the negative pressure and the atmospheric pressure generated by the negative pressure pump with respect to the atmospheric pressure is generated. A pressure pump connectable to the other end of the access container via the sample filtration filter, and a dropping unit for dropping a reagent into the sample tube,
An unsuitable sample collected by the unsuitable sample removing filter by draining the unnecessary liquid present in the sample tube and passing the washing liquid through the unsuitable sample removing filter and the inlet / outlet nozzle and then discarding the washing solution. And a washing means for washing the unsuitable sample removing filter and the loading / unloading nozzle.
各出し入れノズルに対して直列に接続自在な出し入れ容
器とを、それぞれ複数個設ける事により、複数の検体管
への液体の出し入れ作業を同時に行なえる様にした、請
求項１に記載の自動細胞処理装置。2. A plurality of sample tubes are provided according to the number of sample tubes, and a plurality of inlet / outlet containers which can be connected in series to the respective inlet / outlet nozzles are provided. 2. The automatic cell processing apparatus according to claim 1, wherein the automatic cell processing apparatus can be performed simultaneously.
容器とこの試薬を検体管に滴下する為のノズルとを結ぶ
配管の途中に、少量の試薬を貯溜自在な一時貯溜部を、
上記配管と直列に設ける事により、貯蔵容器から取り出
された試薬が、反応温度に近付いてから上記ノズルに送
られる様にした、請求項１〜２の何れかに記載の自動細
胞処理装置。3. A temporary storage part capable of storing a small amount of a reagent is provided in the middle of a pipe connecting a storage container storing the reagent at a temperature other than the reaction temperature and a nozzle for dropping the reagent onto a sample tube.
The automatic cell processing apparatus according to claim 1, wherein a reagent taken out of a storage container is sent to the nozzle after approaching a reaction temperature by being provided in series with the pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2189400A JP2966057B2 (en) | 1990-07-19 | 1990-07-19 | Automatic cell processing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2189400A JP2966057B2 (en) | 1990-07-19 | 1990-07-19 | Automatic cell processing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0476458A JPH0476458A (en) | 1992-03-11 |
| JP2966057B2 true JP2966057B2 (en) | 1999-10-25 |
Family
ID=16240658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2189400A Expired - Fee Related JP2966057B2 (en) | 1990-07-19 | 1990-07-19 | Automatic cell processing device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2966057B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005103642A1 (en) * | 2004-04-23 | 2005-11-03 | The Furukawa Electric Co., Ltd. | Methods of separating, identifying and dispensing specimen and device therefor, and analyzing device method |
| JP2011169886A (en) * | 2010-01-21 | 2011-09-01 | Sysmex Corp | Sample preparation apparatus |
| WO2012039097A1 (en) * | 2010-09-22 | 2012-03-29 | シスメックス株式会社 | Cell analyzer |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8172702B2 (en) | 2000-06-16 | 2012-05-08 | Skyhawke Technologies, Llc. | Personal golfing assistant and method and system for graphically displaying golf related information and for collection, processing and distribution of golf related data |
| US7121962B2 (en) | 2000-12-19 | 2006-10-17 | Reeves G George | Golf round data system with cellular telephone and player help features |
| CN112924365A (en) * | 2021-01-22 | 2021-06-08 | 贝克曼库尔特生物科技（苏州）有限公司 | Fluidic system and sample processing instrument comprising the same |
-
1990
- 1990-07-19 JP JP2189400A patent/JP2966057B2/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005103642A1 (en) * | 2004-04-23 | 2005-11-03 | The Furukawa Electric Co., Ltd. | Methods of separating, identifying and dispensing specimen and device therefor, and analyzing device method |
| JP2009300458A (en) * | 2004-04-23 | 2009-12-24 | Furukawa Electric Co Ltd:The | Specimen analysis device and specimen analysis method |
| US8264674B2 (en) | 2004-04-23 | 2012-09-11 | The Furukawa Electric Co., Ltd. | Methods of separating, identifying and dispensing specimen and device therefor, and analyzing device method |
| US8586890B2 (en) | 2004-04-23 | 2013-11-19 | The Furukawa Electric Co., Ltd. | Methods of separating, identifying and dispensing specimen and device therefor, and analyzing device method |
| JP2011169886A (en) * | 2010-01-21 | 2011-09-01 | Sysmex Corp | Sample preparation apparatus |
| US9046505B2 (en) | 2010-01-21 | 2015-06-02 | Sysmex Corporation | Sample preparation apparatus |
| WO2012039097A1 (en) * | 2010-09-22 | 2012-03-29 | シスメックス株式会社 | Cell analyzer |
| JP2012068108A (en) * | 2010-09-22 | 2012-04-05 | Sysmex Corp | Cell analyzer |
| CN103109184A (en) * | 2010-09-22 | 2013-05-15 | 希森美康株式会社 | Cell Analyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0476458A (en) | 1992-03-11 |
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