JP2023151641A - Grain seed sterilization method using acetic peracid formulation, acetic peracid formulation used for grain seed sterilization treatment and disease control method - Google Patents
Grain seed sterilization method using acetic peracid formulation, acetic peracid formulation used for grain seed sterilization treatment and disease control method Download PDFInfo
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Abstract
Description
本発明は、穀物種子殺菌処理用の過酢酸製剤および該過酢酸製剤を用いる穀物種子殺菌処理方法ならびに病害防除方法に関する。 The present invention relates to a peracetic acid preparation for sterilizing grain seeds, a method for sterilizing grain seeds, and a method for controlling diseases using the peracetic acid preparation.
日本の主要な穀物として、イネ、コムギ、オオムギなどがあげられる。その内、イネ（水稲）の育苗段階において、もみ枯細菌病やいもち病などの種子伝染性の病害を発生させないために、発芽前の種子の殺菌処理を行うことが一般的に行われている。従来、種子の殺菌（消毒）には、化学農薬、温湯に浸漬する温湯処理による方法、または、微生物農薬によるものなどの防除方法があるが、安全面や環境面、薬剤耐性菌の問題、作業上の不便さなどの問題点を有する（特許文献１、２、非特許文献１など）。 Japan's main grains include rice, wheat, and barley. Among these, during the seedling-raising stage of rice (paddy rice), it is common practice to sterilize the seeds before they germinate in order to prevent the occurrence of seed-borne diseases such as rice blast and rice blast. . Traditionally, seeds have been sterilized (disinfected) using chemical pesticides, immersion in warm water, or microbial pesticides, but these methods have faced safety, environmental concerns, problems with drug-resistant bacteria, and problems with handling. This method has problems such as the above inconvenience (Patent Documents 1 and 2, Non-Patent Document 1, etc.).
一方、近年、過酢酸製剤を農業分野に適用する試みが行われている（特許文献３～８）。過酢酸製剤は、過酸化水素と酢酸を適切に混合し、それらの化学平衡反応により形成された過酢酸を用いるもので、過酢酸、過酸化水素、酢酸および水を含む。耐性の強い芽胞に対し、その酸化作用から殺菌用途に用いられてきた過酸化水素よりも有効であること、有機物存在下でも塩素のように殺菌力が低下しないこと、また、酵素やＤＮＡのような生体必須分子を非可逆的に酸化変性させる殺菌メカニズムから耐性菌が出現しにくいことなどの特徴を有する。さらに、過酢酸製剤は、経時変化的に、あるいはｐＨ調整などによって、水、酸素と生分解が容易である酢酸（塩）になることから、環境面や健康面で取扱いの容易な殺菌・消毒剤と考えられる。また、過酢酸製剤は、食肉・鶏肉・野菜の殺菌用の食品添加物として認可されている。 On the other hand, in recent years, attempts have been made to apply peracetic acid preparations to the agricultural field (Patent Documents 3 to 8). Peracetic acid formulations use peracetic acid formed by appropriately mixing hydrogen peroxide and acetic acid and their chemical equilibrium reaction, and include peracetic acid, hydrogen peroxide, acetic acid, and water. It is more effective against highly resistant spores than hydrogen peroxide, which has been used for sterilization due to its oxidizing action, and its sterilizing power does not decrease like chlorine even in the presence of organic matter. Its sterilization mechanism irreversibly oxidizes and denatures biologically essential molecules, making it difficult for resistant bacteria to emerge. Additionally, peracetic acid preparations change over time or through pH adjustment to form acetic acid (salt), which is easily biodegradable with water and oxygen, making them easy to handle and disinfect from an environmental and health standpoint. It is considered to be a drug. Additionally, peracetic acid preparations are approved as food additives for sterilizing meat, poultry, and vegetables.
具体的には、浸種前や催芽前後のイネ種子を、適当な過酢酸濃度で浸漬処理し、イネ種子伝染性の病害を防除する方法（特許文献３）、イネ種子の催芽処理時に適切な濃度範囲の過酢酸を用いる方法（特許文献５）、適切な濃度範囲の過酢酸製剤を実用的な処理時間で用いる方法（特許文献６）、細菌病であるトマト青枯病に対し過酢酸を土壌潅注して防除する方法（特許文献３）、カンキツかいよう病などの細菌病やイチゴうどんこ病などの糸状菌病に対する防除効果を示す方法（特許文献７）、温州ミカンやピーマンなどの果実の糸状菌による貯蔵病害の抑制方法（特許文献８）などが報告されている。 Specifically, a method for controlling rice seed infectious diseases by immersing rice seeds before and after seed immersion or before and after germination in an appropriate concentration of peracetic acid (Patent Document 3); (Patent Document 5), a method of using a peracetic acid preparation with an appropriate concentration range in a practical treatment time (Patent Document 6), A method of controlling bacterial diseases such as citrus canker and filamentous fungal diseases such as strawberry powdery mildew (patent document 7); A method for suppressing storage diseases caused by bacteria (Patent Document 8) has been reported.
農業用過酢酸製剤として３成分（過酢酸、酢酸および過酸化水素）の最適な組成について言及している知見はない。農業用過酢酸製剤として要望される性能として、有効な殺菌活性を有していること、かつ、植物への薬害の無いことが課題となっている。本発明は、過酢酸、酢酸および過酸化水素を含有する過酢酸製剤であって、イネなどの穀物種子の殺菌処理用として、殺菌活性と植物への薬害の観点から最適な過酢酸製剤の組成を見出すことを目的とする。また、該過酢酸製剤を用いる穀物種子殺菌処理方法ならびに細菌病や糸状菌などの病害防除方法を提供することを目的とする。 There is no knowledge that mentions the optimal composition of the three components (peracetic acid, acetic acid, and hydrogen peroxide) for agricultural peracetic acid preparations. The required performance for agricultural peracetic acid preparations is to have effective bactericidal activity and to have no phytotoxicity to plants. The present invention is a peracetic acid preparation containing peracetic acid, acetic acid, and hydrogen peroxide, and the composition of the peracetic acid preparation is optimal from the viewpoint of bactericidal activity and phytotoxicity to plants for use in the sterilization treatment of grain seeds such as rice. The purpose is to find out. Another object of the present invention is to provide a method for sterilizing grain seeds and a method for controlling diseases such as bacterial diseases and filamentous fungi using the peracetic acid preparation.
本発明者らは、穀物種子の殺菌処理用の過酢酸製剤として、過酢酸、酢酸および過酸化水素を含有する過酢酸製剤を調製し、有効な殺菌活性を有し、かつ、植物への薬害の認められない過酢酸製剤を見出し、該過酢酸製剤を用いる穀物種子殺菌処理方法ならびに病害防除方法を開発した。すなわち、本発明は以下を要旨とする。 The present inventors prepared a peracetic acid preparation containing peracetic acid, acetic acid, and hydrogen peroxide as a peracetic acid preparation for the sterilization treatment of grain seeds. We found a peracetic acid preparation that does not allow the above, and developed a method for sterilizing grain seeds and a method for controlling diseases using this peracetic acid preparation. That is, the gist of the present invention is as follows.
１．有効成分として過酢酸を０．０１質量％～１６質量％含有する過酢酸製剤であって、
酢酸と過酢酸の質量比が、酢酸／過酢酸＝１．０～３．１であって、かつ、
過酸化水素と過酢酸の質量比が、過酸化水素／過酢酸＝０．６～３．１である、
穀物種子殺菌処理用の過酢酸製剤。
1. A peracetic acid preparation containing 0.01% to 16% by mass of peracetic acid as an active ingredient,
The mass ratio of acetic acid and peracetic acid is acetic acid/peracetic acid = 1.0 to 3.1, and
The mass ratio of hydrogen peroxide and peracetic acid is hydrogen peroxide/peracetic acid = 0.6 to 3.1.
Peracetic acid formulation for grain seed sterilization treatment.
２．前記過酢酸製剤を１００～１０００倍に希釈し、浸種前処理に用いる穀物種子殺菌処理方法。 2. A grain seed sterilization treatment method in which the peracetic acid preparation is diluted 100 to 1000 times and used for pre-soaking treatment.
３．前記過酢酸製剤を１００～１０００倍に希釈し、催芽時処理に用いる穀物種子殺菌処理方法。 3. A grain seed sterilization treatment method in which the peracetic acid preparation is diluted 100 to 1000 times and used for germination treatment.
４．前記過酢酸製剤を用いて、浸漬処理、散布処理または土壌処理することを特徴とする穀物種子殺菌処理方法。 4. A method for sterilizing grain seeds, which comprises immersing, spraying, or treating soil using the peracetic acid preparation.
５．前記穀物処理殺菌処理方法を用いる、穀物種子の細菌病または糸状菌病の病害防除方法。 5. A method for controlling bacterial or filamentous fungal diseases of grain seeds using the grain processing and sterilization method.
６．前記細菌病が、もみ枯細菌病、褐条病または苗立枯細菌病である、穀物種子の細菌病の病害防除方法。 6. A method for controlling a bacterial disease of grain seeds, wherein the bacterial disease is a bacterial rice blight disease, a brown streak disease, or a bacterial seedling blight disease.
７．前記糸状菌病が、ばか苗病、いもち病またはごま葉枯病である、穀物種子の糸状菌病の病害防除方法。 7. A method for controlling a filamentous fungal disease of grain seeds, wherein the filamentous fungal disease is Bakanae disease, blast disease, or sesame leaf blight.
本発明の過酢酸製剤は、穀物種子の殺菌処理用として最適な組成を有しており、穀物種子に対して糸状菌病または細菌病などの種子感染性病害を防除し、植物に対する薬害の認められない病害防除方法として有効である。 The peracetic acid preparation of the present invention has an optimal composition for the sterilization treatment of grain seeds, and controls seed-borne diseases such as fungal diseases and bacterial diseases on grain seeds, and prevents phytotoxicity on plants. It is effective as a method of controlling diseases that cannot be avoided.
以下、本発明の実施形態について詳細に説明するが、本発明は、以下の実施形態に限定されず、その要旨の範囲内で様々な形態で実施することができる。 Hereinafter, embodiments of the present invention will be described in detail, but the present invention is not limited to the following embodiments and can be implemented in various forms within the scope of the gist thereof.
本発明の過酢酸製剤は、過酸化水素と酢酸を混合して調製することができる。用いる過酸化水素および酢酸の濃度・量は、公知の過酢酸の平衡定数をもとに、得ようと考える過酢酸の濃度に応じて、適宜選択すればよい。過酸化水素と酢酸を混合し、撹拌して室温で数日間静置すると、両者の化学平衡反応によって過酢酸が生成され、過酢酸製剤が調製される。 The peracetic acid formulation of the present invention can be prepared by mixing hydrogen peroxide and acetic acid. The concentration and amount of hydrogen peroxide and acetic acid to be used may be appropriately selected based on the known equilibrium constant of peracetic acid, depending on the concentration of peracetic acid to be obtained. When hydrogen peroxide and acetic acid are mixed, stirred, and allowed to stand at room temperature for several days, peracetic acid is produced by a chemical equilibrium reaction between the two, and a peracetic acid preparation is prepared.
本発明の過酢酸製剤は、そのまま、もしくは、過酢酸濃度が適切になるように希釈して使用する。具体的には、有効成分として過酢酸を０．０１質量％～１６質量％含有するように希釈することによって得ることができる。また、本発明における過酢酸製剤は、希釈して使用することによって、実際の使用する現場においても安全に使用できるものである。 The peracetic acid preparation of the present invention is used as it is or after being diluted to an appropriate peracetic acid concentration. Specifically, it can be obtained by diluting it so that it contains 0.01% to 16% by mass of peracetic acid as an active ingredient. Furthermore, the peracetic acid preparation of the present invention can be used safely in actual use by diluting it.
本発明の過酢酸製剤において、過酢酸、酢酸、および過酸化水素の濃度の調整は、公知の方法を用いて行ってよい。例えば、カタラーゼなどの添加剤を使用して過酸化水素の濃度を低減させてもよい。 In the peracetic acid formulation of the present invention, the concentrations of peracetic acid, acetic acid, and hydrogen peroxide may be adjusted using known methods. For example, additives such as catalase may be used to reduce the concentration of hydrogen peroxide.
本発明の過酢酸製剤による殺菌効果は、過酢酸の濃度と処理時間、温度、また対象とする病害細菌やカビの種類に依存するため、それらを勘案して適切な濃度、処理時間、処理温度を設定する必要がある。これは過酢酸製剤に含有する過酢酸の殺菌メカニズムによる。生体浸透性および酸化力の強い過酢酸が、病原菌体の膜、酵素、ＤＮＡなど生命維持活動に必須な要素を酸化により不可逆的に変質または不活性化させてしまうため、殺菌効率が良くかつ耐薬性菌が出にくい。 The bactericidal effect of the peracetic acid preparation of the present invention depends on the concentration of peracetic acid, treatment time, and temperature, as well as the types of target disease bacteria and molds. need to be set. This is due to the bactericidal mechanism of peracetic acid contained in peracetic acid preparations. Peracetic acid, which is biopermeable and has strong oxidizing power, irreversibly alters or inactivates elements essential for life-sustaining activities such as the membranes, enzymes, and DNA of pathogenic bacteria through oxidation, so it has high sterilization efficiency and is chemically resistant. Sexual bacteria are hard to come out.
本発明の過酢酸製剤を希釈して使用する場合、過酢酸濃度０．０１質量％～１６質量％の過酢酸製剤を、１００～１０００倍に希釈して用いることが好ましい。その際の過酢酸濃度としては、１００ｐｐｍ～１００００ｐｐｍであることが好ましく、１００～２０００ｐｐｍであることがより好ましい。 When using the peracetic acid preparation of the present invention diluted, it is preferable to use a peracetic acid preparation having a peracetic acid concentration of 0.01% by mass to 16% by mass, diluted 100 to 1000 times. The peracetic acid concentration at this time is preferably 100 ppm to 10,000 ppm, more preferably 100 to 2,000 ppm.
本発明の過酢酸製剤は、希釈して穀物種子の殺菌処理方法において用いる場合、浸種前処理または催芽時処理に用いることが好ましい。浸種前処理を行う場合の温度は、１０～１５℃が好ましい。浸種前処理を行う時間は、６０分～４８時間が好ましく、６０分～２４時間がより好ましい。 When the peracetic acid preparation of the present invention is diluted and used in a method for sterilizing grain seeds, it is preferably used for pre-soaking treatment or treatment during germination. The temperature when performing seed soaking pretreatment is preferably 10 to 15°C. The time for performing the pre-soaking treatment is preferably 60 minutes to 48 hours, more preferably 60 minutes to 24 hours.
本発明の過酢酸製剤は、催芽処理時に処理を行う場合は、３０℃～３２℃程度の温水での催芽処理時で行うことが好ましい。催芽処理時を行う時間は、６０分～２４時間が好ましい。 If the peracetic acid preparation of the present invention is to be treated during germination treatment, it is preferably carried out at the time of germination treatment with warm water of about 30°C to 32°C. The time for conducting the germination treatment is preferably 60 minutes to 24 hours.
本発明における、穀物種子の病害の種類としては、細菌病、糸状菌病によるものに対して効果が高い。細菌病としては、もみ枯細菌病、褐条病、または、苗立枯細菌病などがあげられ、もみ枯細菌病に対してより効果が高い。糸状菌病としては、ばか苗病、いもち病、ごま葉枯病があげられ、ばか苗病またはいもち病に対してより効果が高い。 The present invention is highly effective against bacterial diseases and fungal diseases of grain seeds. Bacterial diseases include bacterial rice blight, brown streak disease, and bacterial seedling blight, and are more effective against bacterial rice blight. Examples of filamentous fungal diseases include Bakanae disease, rice blast, and sesame leaf blight, and it is more effective against Bakanae disease or rice blast.
本発明の過酢酸製剤での殺菌処理方法において、種子をそのままで過酢酸製剤中に浸漬処理してもよいが、布袋やネットなどに入れて処理を行なってもよい。特に、高濃度で短時間処理する場合には、過酢酸製剤液中からすばやく出し入れする必要があり、その場合は、液切れ性も考えると、ネットのような目の粗いものに入れて処理を行なうのが好ましい。 In the sterilization method using a peracetic acid preparation of the present invention, the seeds may be immersed as they are in the peracetic acid preparation, but they may also be placed in a cloth bag or net for treatment. In particular, when processing at high concentrations for a short period of time, it is necessary to quickly remove the peracetic acid from the solution. It is preferable to do so.
本発明の過酢酸製剤での殺菌処理を行なった場合、特にイネの種子では、続いて浸種処理、催芽処理を行い、健全に発芽、育苗させることが出来る。過酢酸の殺菌効果に関して、防除価を用いて評価する。防除価は無処理区における細菌や糸状菌などによる病害の被害に対する処理区の防除効果の程度を表す指数であり、一般的に、防除価が高いほど防除効果があることを示す。一般的に、
防除価＝（１００－（処理区の発病度／無処理区の発病度）×１００）の式によって計算する。防除価が高いほど防除効果があることを示す。
When sterilization treatment is performed using the peracetic acid preparation of the present invention, rice seeds in particular can be subsequently subjected to seed soaking treatment and germination treatment to ensure healthy germination and seedling growth. The bactericidal effect of peracetic acid is evaluated using the control value. The control value is an index representing the degree of control effect of the treated area against damage caused by diseases such as bacteria and filamentous fungi in the untreated area, and generally, the higher the control value, the more effective the control is. Typically,
Control value is calculated using the formula: (100 - (infectivity in treated area/incidence in non-treated area) x 100). The higher the control value, the greater the control effect.
以下、本発明を実施例により具体的に説明するが、本発明はこれらの実施例に限定されない。 EXAMPLES Hereinafter, the present invention will be specifically explained with reference to Examples, but the present invention is not limited to these Examples.
［実施例１］ [Example 1]
［製剤実施例１および２、製剤比較例１および２］
過酢酸、酢酸および過酸化水素の濃度が、それぞれ表１に記載した組成の濃度になるように調製し、製剤実施例１（過酢酸Ｂ）、製剤実施例２（ＭＯ－１、オキシペール１００（製品名、保土谷化学工業株式会社製））を用意した。また、比較対照として、製剤比較例１（過酢酸Ａ）および市販のイネ種子消毒剤（商品名：テクリードＣフロアブル、成分：イプコナゾール、水酸化第二銅）を用意した。
[Formulation Examples 1 and 2, Formulation Comparative Examples 1 and 2]
The concentrations of peracetic acid, acetic acid, and hydrogen peroxide were adjusted to the concentrations of the compositions listed in Table 1. Product name (manufactured by Hodogaya Chemical Industry Co., Ltd.)) was prepared. In addition, as a comparison control, Formulation Comparative Example 1 (peracetic acid A) and a commercially available rice seed disinfectant (trade name: Techled C Flowable, ingredients: ipconazole, cupric hydroxide) were prepared.
［実施例１、実施例２、比較例１および比較例２］
＜イネもみ枯細菌病防除試験＞
［浸種前処理］
表１の各過酢酸製剤を水で希釈して水溶液を濃度（過酢酸濃度：１００ｐｐｍ）調製した。この水溶液２５ｍＬを１００ｍＬ容ビーカーに入れ、これにイネもみ枯細菌病に汚染されたイネ籾１２ｇを浸漬処理し、防除効果を調べた。浸漬（浸種前処理）は、１５℃で２４時間行った。
浸漬（浸種前処理）後のイネ籾は、浸種（３日間、１５℃）および催芽（３２℃）を２４時間実施した。発芽した籾を育苗箱の１／１２サイズのプラスチックケースに培土を入れて播種し、３２℃で２日間保った後、温室内で管理した。
播種１４日後に、発病苗率および発病度を下式により算出し、防除価を算出した。
発病指数
３：枯死苗
２：重症苗（萎凋苗）
１：軽症苗（黄白化苗、奇形苗）
０：健全
発病度＝｛Σ（発病程度別苗数）×（発病指数）÷（調査苗数×３）｝×１００
薬害は、発芽数を計測し、無処理区の発芽数を１００として、
以下の式から発芽阻害率（％）を算出した。
発芽阻害率（％）＝（１－（処理区の発芽数／無処理区の発芽数））×１００ （％）
比較のため、製剤比較例１として「過酢酸Ａ」（酢酸／過酢酸＝４．６（質量比））、および、製剤比較例２として市販のイネ種子消毒剤（商品名：テクリードＣフロアブル、成分：イプコナゾール、水酸化第二銅）を用いた。防除価による効果判定、および、発芽阻害率（％）による薬害判定は、表２の基準で評価した。試験結果を表３に示す。
[Example 1, Example 2, Comparative Example 1 and Comparative Example 2]
<Rice rice blight bacterial disease control test>
[Seeding pretreatment]
Each peracetic acid preparation in Table 1 was diluted with water to prepare an aqueous solution (peracetic acid concentration: 100 ppm). 25 mL of this aqueous solution was put into a 100 mL beaker, and 12 g of rice grains contaminated with rice blight was immersed therein to examine the control effect. Soaking (soaking pretreatment) was carried out at 15° C. for 24 hours.
The rice rice after soaking (pre-seeding treatment) was subjected to seed soaking (3 days, 15°C) and germination (32°C) for 24 hours. Germinated paddy was sown in a 1/12-sized plastic case of a seedling box with culture soil, kept at 32°C for 2 days, and then managed in a greenhouse.
Fourteen days after sowing, the rate of diseased seedlings and disease severity were calculated using the following formula, and the control value was calculated.
Disease index 3: Dead seedlings 2: Severe seedlings (wilted seedlings)
1: Mild seedlings (yellowing and malformed seedlings)
0: Healthy disease severity = {Σ (number of seedlings by disease severity) x (disease index) ÷ (number of surveyed seedlings x 3)} x 100
For chemical damage, the number of germination was measured, and the number of germination in the untreated area was set as 100.
The germination inhibition rate (%) was calculated from the following formula.
Germination inhibition rate (%) = (1 - (number of germination in treated area / number of germination in untreated area)) x 100 (%)
For comparison, "Peracetic acid A" (acetic acid/peracetic acid = 4.6 (mass ratio)) was used as Formulation Comparative Example 1, and a commercially available rice seed disinfectant (trade name: Techled C Flowable, Ingredients: ipconazole, cupric hydroxide) were used. Efficacy evaluation based on control value and phytotoxicity evaluation based on germination inhibition rate (%) were evaluated based on the criteria shown in Table 2. The test results are shown in Table 3.
表３から、本発明の過酢酸製剤を用いたものは、比較例より薬害が認められないことがわかった。 From Table 3, it was found that the products using the peracetic acid preparation of the present invention showed no drug damage compared to the comparative example.
［実施例３、実施例４、比較例３および比較例２］
＜イネもみ枯細菌病防除試験＞
［催芽時処理］
実施例１および２と同様に、過酢酸、酢酸および過酸化水素の濃度が、それぞれ表１に記載した組成の濃度になるように調製し、製剤実施例１（過酢酸Ｂ）、製剤実施例２（ＭＯ－１、オキシペール１００（製品名、保土谷化学工業株式会社製））を用意した。また、比較対照として、製剤比較例１（過酢酸Ａ）および市販のイネ種子消毒剤（商品名：テクリードＣフロアブル、成分：イプコナゾール、水酸化第二銅）を用意した。
[Example 3, Example 4, Comparative Example 3 and Comparative Example 2]
<Rice rice blight bacterial disease control test>
[Processing during germination]
Similarly to Examples 1 and 2, the concentrations of peracetic acid, acetic acid, and hydrogen peroxide were adjusted to the concentrations of the compositions listed in Table 1, respectively, and Formulation Example 1 (Peracetic acid B) and Formulation Example 2 (MO-1, Oxiper 100 (product name, manufactured by Hodogaya Chemical Industry Co., Ltd.)) was prepared. In addition, as a comparison control, Formulation Comparative Example 1 (peracetic acid A) and a commercially available rice seed disinfectant (trade name: Techled C Flowable, ingredients: ipconazole, cupric hydroxide) were prepared.
各過酢酸製剤の水溶液を３濃度（２５０、３３３、５００ｐｐｍ）調製した。本水溶液４５ｍＬを１００ｍＬ容ビーカーに入れ、これにイネもみ枯細菌病に汚染されたイネ籾１２ｇを浸漬（催芽時処理）し、防除効果を調べた。イネ籾は１５℃で浸種されたものを用いた。浸漬（催芽時処理）は、３２℃で２４時間行った。
発芽した籾を育苗箱の１／１２サイズのプラスチックケースに培土を入れて播種し、３２℃で２日間保った後、温室内で管理した。
播種１４日後に、発病苗率及び、実施例１および２と同様に、下式に従い発病度を算出し、防除価を算出した。
発病指数
３：枯死苗
２：重症苗（萎凋苗）
１：軽症苗（黄白化苗、奇形苗）
０：健全
発病度＝｛Σ（発病程度別苗数）×（発病指数）÷（調査苗数×３）｝×１００
薬害については、発芽数を計測し、無処理区の発芽数を100として、以下の式から発芽阻害率(％)を算出した。
発芽阻害率（％）＝（１－（処理区の発芽数／無処理区の発芽数））×１００ （％）
比較のため、製剤比較例１として「過酢酸Ａ」（酢酸／過酢酸＝４．６（質量比））、および、製剤比較例２として市販のイネ種子消毒剤（商品名：テクリードＣフロアブル、成分：イプコナゾール、水酸化第二銅）を用いた。防除価による効果判定、および、発芽阻害率（％）による薬害判定は、表２の基準で評価した。試験結果を表４に示す。
Aqueous solutions of each peracetic acid formulation were prepared at three concentrations (250, 333, and 500 ppm). 45 mL of this aqueous solution was put into a 100 mL beaker, and 12 g of rice grains contaminated with rice blight was immersed in it (treatment during germination) to examine the control effect. The rice used was seed soaked at 15°C. Soaking (treatment during germination) was performed at 32°C for 24 hours.
Germinated paddy was sown in a 1/12-sized plastic case of a seedling box with culture soil, kept at 32°C for 2 days, and then managed in a greenhouse.
Fourteen days after sowing, the rate of diseased seedlings and the severity of the disease were calculated according to the following formula in the same manner as in Examples 1 and 2, and the control value was calculated.
Disease index 3: Dead seedlings 2: Severe seedlings (wilted seedlings)
1: Mild seedlings (yellowing and malformed seedlings)
0: Healthy disease severity = {Σ (number of seedlings by disease severity) x (disease index) ÷ (number of surveyed seedlings x 3)} x 100
Regarding chemical damage, the number of germination was measured, and the germination inhibition rate (%) was calculated from the following formula, setting the number of germination in the untreated plot as 100.
Germination inhibition rate (%) = (1 - (number of germination in treated area / number of germination in untreated area)) x 100 (%)
For comparison, "Peracetic acid A" (acetic acid/peracetic acid = 4.6 (mass ratio)) was used as Formulation Comparative Example 1, and a commercially available rice seed disinfectant (trade name: Techled C Flowable, Ingredients: ipconazole, cupric hydroxide) were used. Efficacy evaluation based on control value and phytotoxicity evaluation based on germination inhibition rate (%) were evaluated based on the criteria shown in Table 2. The test results are shown in Table 4.
表４から、本発明の過酢酸製剤を用いたものは、上記の処理濃度で薬害が認められず、比較例より高い防除効果を有することがわかった。 From Table 4, it was found that the products using the peracetic acid preparation of the present invention showed no phytotoxicity at the above-mentioned treatment concentration and had a higher pesticidal effect than the comparative example.
本発明の過酢酸製剤は、殺菌活性と穀物などの植物に対する薬害に関する観点から、過酢酸製剤の最適な３成分（過酢酸、酢酸および過酸化水素）の組成を有しており、本発明の過酢酸製剤を用いる穀物種子の殺菌処理方法は、穀物種子に対して糸状菌病または細菌病などの種子感染性病害を防除し、穀物などの植物に対する薬害の認められない病害防除方法として有効である。 The peracetic acid preparation of the present invention has the optimal composition of three components (peracetic acid, acetic acid, and hydrogen peroxide) for a peracetic acid preparation from the viewpoint of bactericidal activity and phytotoxicity to plants such as grains. The method of sterilizing grain seeds using a peracetic acid preparation is effective for controlling seed-borne diseases such as fungal diseases and bacterial diseases on grain seeds, and as a disease control method that does not cause phytotoxicity on plants such as grains. be.
Claims (7)
酢酸と過酢酸の質量比が、酢酸／過酢酸＝１．０～３．１であって、かつ、
過酸化水素と過酢酸の質量比が、過酸化水素／過酢酸＝０．６～３．１である、
穀物種子殺菌処理用の過酢酸製剤。 A peracetic acid preparation containing 0.01% to 16% by mass of peracetic acid as an active ingredient,
The mass ratio of acetic acid and peracetic acid is acetic acid/peracetic acid = 1.0 to 3.1, and
The mass ratio of hydrogen peroxide and peracetic acid is hydrogen peroxide/peracetic acid = 0.6 to 3.1.
Peracetic acid formulation for grain seed sterilization treatment.
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