JP2021516552A - 高速ポリメラーゼ連鎖反応分析プレート - Google Patents
高速ポリメラーゼ連鎖反応分析プレート Download PDFInfo
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Abstract
Description
(b)1次PCRが進行される過程は、図9の実施例で上述したように、逆止弁Vの動作により反応部上でPCR反応が行われる。
(c)以後、1次PCRが終結すると、1次仕切弁D1を押圧しているピストンPG1を上に持ち上げて正圧で満たされていた1次PCR反応部の溶液が流路に沿って第1仕切弁D1を通過して精製部FTを過ぎて第2のPCR反応部W2に移動する。この際、最大限溶液を全部移動させるために、後方ヒーティングブロックHB2を持ち上げ、前方ヒーティングブロック1 HB1で押圧して溶液を完全に移動させた後、1次仕切弁D1のピストンPG1を押圧して流路を遮断する。以後、高温と低温のヒーティングブロックHB2を交互に押圧して2次PCRサイクルを進行して2次PCRを行う。この際、第1仕切弁D1および第2仕切弁D2は、全部閉弁していなければならない。
(d)2次PCRが終結した後、第2仕切弁D2を開放してラテラルフロー分析部LSのローディングパッドに2次PCR溶液が流れるようにする。これを通じて、2次PCR反応で生成された単一らせん鎖のDNAがターゲットプローブ核酸に会って移動を止めると、各バンドのラベルを検出してターゲットを検出することができる。
W 反応部
F 弾性フィルム
W1 第1のPCR反応部
W2 第2のPCR反応部
W3 第3のPCR反応部
J 流路部
J2 第2流路部
J3 第3流路部
V 逆止弁
Va 経路形成ブロック
K 逆止弁溝
Ha 流入溝
D、D1、D2 仕切弁
LS ラテラルフロー分析部
HB、HB1、HB2 ヒーティングブロック
FT 精製部
h1 注入ホール
hd1、hd2 ホール
L1 ローディングパッド
L2 プローブ固定バンド
L3 吸着パッド
Claims (16)
- 注入口が形成されたベース基板(S);
前記ベース基板(S)上に、前記注入口を含む閉鎖線形態で弾性を有するシーリングフィルムが融着して密閉された構造で具現される反応部(W);および
前記反応部(W)の注入口と連結される流路部(J)と、前記反応部(W)との間に形成される仕切弁(D);
を含む高速ポリメラーゼ連鎖反応分析プレート。 - 注入口が形成されたベース基板(S);
前記ベース基板(S)上に注入口を含む閉鎖線形態で弾性を有するシーリングフィルムが融着して密閉された反応部(W);および
前記反応部(W)の前記注入口と連結される流路部(J)と、前記反応部(W)に注入された溶液の逆流を防止する逆止弁(V)とを含む弁部;
を含む高速ポリメラーゼ連鎖反応分析プレート。 - 前記ベース基板上の前記反応部(W)は、
閉鎖線形態で弾性を有するシーリングフィルムが融着して密閉された第1のPCR反応部(W1)から離隔する第2のPCR反応部(W2);をさらに含み、
第1のPCR反応部(W1)と第2のPCR反応部(W2)は、前記ベース基板の下部に具現される第2流路部(J2)を媒介として連通し、
前記反応部(W)と前記第2反応部(W2)との間の第2流路を通した流体の流れを仕切制御する仕切弁(D);
をさらに含むことを特徴とする、請求項1または2に記載の高速ポリメラーゼ連鎖反応分析プレート。 - 前記第2のPCR反応部は、
複数個の第2のPCR反応部が第2注入口を含む閉鎖線形態で弾性を有するシーリングフィルムが融着して形成され;
前記第1のPCR反応部の排出口から始まって分岐点で複数個の流路に分岐して複数の第2のPCR反応部の注入口に並列連結される第2流路部;
をさらに含むことを特徴とする、請求項3に記載の高速ポリメラーゼ連鎖反応分析プレート。 - 前記ベース基板の反応部(W)の一端に排出口が形成され、
前記排出口と連結される第2流路部を通じて連通するラテラルフロー分析部(LS);
前記ラテラルフロー分析部(LS)は、核酸プローブが固定されたラテラルフロー分析モジュールと第2注入口を含み、閉鎖線形態で弾性を有するシーリングフィルムが融着して形成され、反応部の排出口とラテラルフロー分析部の注入口を連結する第2流路部の溶液の流れを仕切る仕切弁(D);
をさらに含むことを特徴とする、請求項1または2に記載の高速ポリメラーゼ連鎖反応分析プレート。 - 前記第2のPCR反応部(W2)から離隔して配置され、第2排出口が形成され、第3注入口と核酸プローブが固定されたラテラルフロー分析モジュールを含むラテラルフロー分析部(LS)が閉鎖線形態で弾性を有するシーリングフィルムが融着して形成され、
前記第2のPCR反応部(W2)の第2排出口とラテラルフロー分析部の第3注入口を連結する第3流路部;および
前記第3流路部の溶液の流れを仕切る第2仕切弁(D2);
をさらに含むことを特徴とする、請求項3に記載の高速ポリメラーゼ連鎖反応分析プレート。 - 前記逆止弁(V)は、弾性体として流路に注入される溶液の圧力により開放される形態であり、
シーリングフィルムの内側と接触する表面は、溶液が染み込むことができるように円錐型または上部の直径が1/2以下である2段円筒形または上部面に突起が形成されている円筒形で具現され、
前記逆止弁の下部面(V2)は、前記ベース基板(S)上に注入される面と密着するようになめらかな鏡面で具現されることを特徴とする、請求項1または2に記載の高速ポリメラーゼ連鎖反応分析プレート。 - 前記仕切弁(D)は、
前記仕切弁の入口と出口を含む閉鎖線形態で前記弾性を有するシーリングフィルムにより融着して形成され、前記入口から入る溶液の圧力によりシーリングフィルムが伸びて前記出口に排出されて開通し、
前記シーリングフィルムの上部を弁圧着部が押して弾性を有するシーリングフィルムとベース基板を密着させて前記仕切弁の内側空間のすべての溶液が排出されることによって入口と出口が閉鎖されることを特徴とする、請求項1に記載の高速ポリメラーゼ連鎖反応分析プレート。 - 前記ベース基板と弾性を有するシーリングフィルムが融着される閉鎖線は、0.5mm〜2mmの線幅で融着することを特徴とする、請求項1または2に記載の高速ポリメラーゼ連鎖反応分析プレート。
- 前記ベース基板は、
弾性を有するシーリングフィルムが融着する閉鎖線がベース基板(S)から2mm以下の高さで突出して形成されることを特徴とする、請求項1または2に記載の高速ポリメラーゼ連鎖反応分析プレート。 - 前記のラテラルフロー分析モジュールは、核酸プローブが固定されたラテラルフローペーパー構造であって、始端にローディングパッド、末端に吸収パッドがそれぞれ付着しており、溶液の流れと垂直方向に多数の線形アレイ形態で1つ以上の核酸プローブが固定されていることを特徴とする、請求項5に記載の高速ポリメラーゼ連鎖反応分析プレート。
- 前記反応部の注入口に隣接して、一対以上のプライマー、またはプライマー/プローブ乾燥物がコーティングされており、ポリメラーゼが含まれたターゲット核酸溶液の注入により流路部と反応器内部の空気が圧縮されてターゲット核酸溶液と前記乾燥物とが混ざり、前記弾性を有するシーリングフィルムが凸状に伸び、
前記弾性を有するシーリングフィルムの凸状のフィルム面を圧着ブロックで押圧する操作を繰り返すことによって、注入された溶液が反応部内で動いて前記PCR反応水溶液を均一にすることを特徴とする、請求項1または2に記載の高速ポリメラーゼ連鎖反応分析プレート。 - 前記反応部の注入口と隣接して、1つ以上のプライマー、またはプライマー/プローブが含まれたPCR混合乾燥物がコーティングされており、ターゲット核酸溶液の注入によって流路部と反応器内部の空気が圧縮されて溶液と乾燥物とが混ざり、前記弾性を有するシーリングフィルムが凸状に伸び、前記弾性を有するシーリングフィルムの前記弾性を有するシーリングフィルムの凸状のフィルム面を圧着ブロックで押圧する操作を繰り返すことによって、注入された溶液を移動させて前記PCR反応水溶液を均一にすることを特徴とする、請求項1または2に記載の高速ポリメラーゼ連鎖反応分析プレート。
- 前記第2反応部にネスティッドPCRのためのプライマー/プローブ、またはプライマーとDNA検出用蛍光染料が含まれたPCR混合乾燥物がコーティングされていることを特徴とする、請求項3に記載の高速ポリメラーゼ連鎖反応分析プレート。
- 前記第2反応部(W)に増幅されたDNAの片方の鎖に相補的な5’位置にラベリングされたプライマーとDNAポリメラーゼ反応のための混合物が乾燥されており、混成化分析モジュールのプローブは、前記ラベリングされたプライマーにより合成される単一らせんDNAと相補的な塩基配列を有して固相に固定されていることを特徴とする、請求項5に記載の高速ポリメラーゼ連鎖反応分析プレート。
- ラベリングされたネスティッドプライマーは、
蛍光、化学発光、金ナノ粒子でラベリングされたことを特徴とする、請求項12に記載の高速ポリメラーゼ連鎖反応分析プレート。
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