JP2019189571A - 廃用性筋萎縮抑制剤及び廃用性筋萎縮抑制用食品組成物 - Google Patents
廃用性筋萎縮抑制剤及び廃用性筋萎縮抑制用食品組成物 Download PDFInfo
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- JP2019189571A JP2019189571A JP2018085635A JP2018085635A JP2019189571A JP 2019189571 A JP2019189571 A JP 2019189571A JP 2018085635 A JP2018085635 A JP 2018085635A JP 2018085635 A JP2018085635 A JP 2018085635A JP 2019189571 A JP2019189571 A JP 2019189571A
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- muscular atrophy
- eriocitrin
- muscle
- atrophy
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Abstract
Description
(レモン果皮抽出物の調製)
レモン果皮抽出物は以下の方法で調製した。インライン搾汁後のレモン果皮に2倍量の水を加え、25℃で2時間抽出した。得られた抽出液を珪藻土濾過し、次いで濾液をMF濾過した。得られた濾液を、Brix値40°になるまで減圧濃縮した。Brix値を参考にして算出した可溶性固形分量の2倍量のデキストリン(商品名:NSD300,サンエイ糖化製)を得られた濃縮液に添加した。次いで、デキストリンを添加した濃縮液を凍結乾燥して粉末化した。得られた粉末をレモン果皮抽出物(エリオシトリン0.5w/w%含有)として試験に供した。
日本エスエルシー社より購入した雄性C57BL/6NCrSlcマウスを試験に供した。マウスは、温度24±1℃、湿度48±4%、12時間の明暗サイクル(明期8:00−20:00)の環境下で飼育した。
坐骨神経を切除することで、不動化による筋萎縮(廃用性筋萎縮)を誘導させたマウスを用いて、レモン果皮抽出物の廃用性筋萎縮抑制作用を評価した。
腓腹筋量及び腓腹筋維持率の評価結果を表1及び図1に示す。表1及び図1に示す腓腹筋量及び腓腹筋維持率の値は、平均値±標準誤差(n=5〜6)である。統計解析はJMP 13(SAS)ソフトを用いた。腓腹筋量はTukeyの検定で多重比較を行い、また腓腹筋維持率はStudentのt検定で2群比較を行い、危険率5%未満を有意とした。
(エリオシトリンの調製)
エリオシトリンは以下の方法で調製した。レモン果皮抽出物(粉末)に酢酸エチルと蒸留水を加えて分配し、酢酸エチル画分と水画分を得た。水画分を回収した後、この水画分にn−ブタノールを加えて分配し、n−ブタノール画分と水画分を得た。n−ブタノール画分を回収した後、このn−ブタノール画分を中圧分取液体クロマトグラフ(LC)により分画し、エリオシトリンを多く含む画分を回収した。次いで、回収した画分をリサイクル分取LCにより分画して、エリオシトリンを精製した。精製したエリオシトリンを、高速液体クロマトグラフ(HPLC)により分析した結果、純度は95%以上であった。
試験食として、AIN−93Mの0.25w/w%を精製したエリオシトリンで置換した粉末飼料を使用したこと、及び対照食として、AIN−93Mの0.25w/w%をデキストリン(商品名:NSD300,サンエイ糖化製)で置換した粉末飼料を使用したこと以外は、実施例1と同様の手順で、腓腹筋量及び腓腹筋維持率を評価した。
腓腹筋量及び腓腹筋維持率の評価結果を表5及び図2に示す。表5及び図2に示す腓腹筋量及び腓腹筋維持率の値は、平均値±標準誤差(n=7)である。統計解析はJMP 13(SAS)ソフトを用いた。腓腹筋量はTukeyの検定で多重比較を行い、また腓腹筋維持率はStudentのt検定で2群比較を行い、危険率5%未満を有意とした。
(クエン酸の廃用性筋萎縮抑制作用の評価)
試験食として、AIN−93Mの1.0w/w%をクエン酸で置換した粉末飼料を使用したこと、及び対照食として、AIN−93Mの1.0w/w%をデキストリン(商品名:NSD300,サンエイ糖化製)で置換した粉末飼料を使用したこと以外は、実施例1と同様の手順で、腓腹筋量及び腓腹筋維持率を評価した。
腓腹筋量及び腓腹筋維持率の評価結果を表6及び図3に示す。表6及び図3に示す腓腹筋量及び腓腹筋維持率の値は、平均値±標準誤差(n=7〜8)である。統計解析はJMP 13(SAS)ソフトを用いた。腓腹筋量はTukeyの検定で多重比較を行い、また腓腹筋維持率はStudentのt検定で2群比較を行い、危険率5%未満を有意とした。
Claims (6)
- エリオシトリンを有効成分として含有する、廃用性筋萎縮抑制剤。
- エリオシトリンを含む柑橘類抽出物を有効成分として含有する、廃用性筋萎縮抑制剤。
- 前記柑橘類抽出物がレモン果皮抽出物である、請求項2に記載の廃用性筋萎縮抑制剤。
- エリオシトリンを有効成分として含有する、廃用性筋萎縮抑制用食品組成物。
- エリオシトリンを含む柑橘類抽出物を有効成分として含有する、廃用性筋萎縮抑制用食品組成物。
- 前記柑橘類抽出物がレモン果皮抽出物である、請求項5に記載の廃用性筋萎縮抑制用食品組成物。
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JPWO2013099982A1 (ja) * | 2011-12-26 | 2015-05-11 | 森永乳業株式会社 | 筋萎縮抑制剤 |
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JP2008156294A (ja) * | 2006-12-25 | 2008-07-10 | Lion Corp | 筋芽細胞活性化剤 |
JPWO2013099982A1 (ja) * | 2011-12-26 | 2015-05-11 | 森永乳業株式会社 | 筋萎縮抑制剤 |
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