JP2018020981A - Composition for improving intestinal environment - Google Patents
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- JP2018020981A JP2018020981A JP2016153805A JP2016153805A JP2018020981A JP 2018020981 A JP2018020981 A JP 2018020981A JP 2016153805 A JP2016153805 A JP 2016153805A JP 2016153805 A JP2016153805 A JP 2016153805A JP 2018020981 A JP2018020981 A JP 2018020981A
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Abstract
Description
本発明は、腸内環境改善用組成物に関する。 The present invention relates to a composition for improving the intestinal environment.
近年、腸内細菌叢（腸内フローラ）と健康との関係について研究が進んでいる。特に、次世代シーケンサーを用いた研究により、腸内フローラを構成する菌種のバランスが崩れると、多様な疾患が引き起こされることが分かってきた。つまり、腸内フローラの菌種のバランスが健康維持に極めて重要であるとの知見が得られてきている。このため、菌そのもの、あるいは低分子化合物などを投与するなどして、腸内フローラの構成を変化させ、疾患を治療・予防したり、健康を維持しようとするという試みも始まっている。 In recent years, research has been conducted on the relationship between intestinal flora (intestinal flora) and health. In particular, research using a next-generation sequencer has revealed that various diseases are caused when the balance of the bacterial species constituting the intestinal flora is lost. That is, the knowledge that the balance of the bacterial species of the intestinal flora is extremely important for health maintenance has been obtained. For this reason, attempts have been made to change the composition of the intestinal flora by treating bacteria themselves or low molecular weight compounds to treat / prevent diseases and maintain health.
また、日常摂取する食事の内容によって腸内細菌のバランスが異なることが知られている。（ｉ）脂肪分や動物性タンパクの多い食事を摂る人と、（ｉｉ）野菜中心で炭水化物の多い食事を摂る人とでは、腸内に存在する微生物の比率が異なることが分かっている。特に、前記（ｉ）（ｉｉ）の人の腸内の特定の種の細菌の比率に基づくと、二つのカテゴリー（すなわち、エンテロタイプ：ｅｎｔｅｒｏｔｙｐｅ）に分けられる。具体的には、前記（ｉ）の人ではバクテロイデス（Ｂａｃｔｅｒｏｉｄｅｓ）属の比率が高く、一方前記（ｉｉ）の人ではプレボテラ（Ｐｒｅｖｏｔｅｌｌａ）属の比率が高い（非特許文献１）。 In addition, it is known that the balance of intestinal bacteria varies depending on the content of meals taken daily. It has been found that the ratio of microorganisms present in the intestine is different between (i) a person who eats a diet rich in fat and animal protein, and (ii) a person who eats a diet rich in carbohydrates mainly in vegetables. In particular, based on the proportion of bacteria of a specific species in the human intestine of (i) (ii) above, it can be divided into two categories (ie, enterotype). Specifically, the ratio of the genus Bacteroides is high in the person (i), while the ratio of the genus Prevotella is high in the person (ii) (Non-patent Document 1).
さらには、地中海式食事のような野菜を中心とした食物繊維が多い食事は、腸内細菌叢に良い影響をもたらし、健康レベルを上げることも知られている。 Furthermore, it is also known that a diet rich in dietary fiber centered on vegetables, such as a Mediterranean diet, has a positive effect on the gut microbiota and increases the level of health.
これらのことから、より健康的な生活を送るためには、腸内フローラにおいて、プレボテラ属の菌の占有比率を高め、腸内環境を改善することが重要と考えられる。 From these facts, in order to live a healthier life, it is considered important to improve the intestinal environment by increasing the occupation ratio of Prebotella bacteria in the intestinal flora.
本発明は、新規な腸内環境改善方法の提供を課題とする。 An object of the present invention is to provide a novel method for improving the intestinal environment.
本発明者らは、β−クリプトキサンチンが腸内フローラにおけるプレボテラ属の菌の比率を高めることを見出し、さらに改良を重ねて本発明を完成させるに至った。 The present inventors have found that β-cryptoxanthin increases the ratio of Prevotella spp. In the intestinal flora, and further improved it to complete the present invention.
本発明は例えば以下の項に記載の主題を包含する。
項１．
β−クリプトキサンチンを含有する腸内環境改善用組成物。
項２．
β−クリプトキサンチンを含有する、プレボテラ属菌の腸内フローラ占有割合を高めるための組成物。
項３．
プレボテラ属菌の腸内フローラ占有割合を高めるための、項１に記載の組成物。
項４．
医薬品組成物又は食品組成物である、項１〜３のいずれかに記載の組成物。
The invention encompasses, for example, the subject matter described in the following sections.
Item 1.
A composition for improving the intestinal environment, comprising β-cryptoxanthin.
Item 2.
A composition for increasing the intestinal flora occupation ratio of Prevotella spp., comprising β-cryptoxanthin.
Item 3.
Item 2. The composition according to Item 1, for increasing an intestinal flora occupation ratio of Prevotella spp.
Item 4.
Item 4. The composition according to any one of Items 1 to 3, which is a pharmaceutical composition or a food composition.
本発明によれば、健常者はもちろん腸内環境が優れない者であっても腸内環境を改善及び／又は良好に維持することができる。特に、本発明では、β−クリプトキサンチンが腸内フローラにおけるプレボテラ属の菌の比率を高めるため、プレボテラ属の菌の比率が比較的低い者（例えば脂肪分や動物性タンパクの多い食事を主に摂取する人）に特に好適である。 According to the present invention, the intestinal environment can be improved and / or well maintained even by a healthy person as well as a person who is not excellent in the intestinal environment. In particular, in the present invention, β-cryptoxanthin increases the ratio of Prevotella spp. In the intestinal flora, so that the ratio of Prevotella spp. Is relatively low (for example, a diet high in fat or animal protein). It is particularly suitable for those who take it.
本発明に包含される組成物（例えば上記項１〜４に記載の組成物）は、腸内改善のために用いることができる。当該組成物は、β−クリプトキサンチンを含む。β−クリプトキサンチンは、例えばミカンやグレープフルーツなどの柑橘類に多く含まれる化合物であり、特に温州ミカンに多く含まれる。本発明に用いるβ−クリプトキサンチンは、特に制限はされず、例えば柑橘類（特に好ましくは温州ミカン）由来のものでもよいし、微生物から抽出されたものや合成品であってもよい。温州ミカンなどの柑橘類由来のものを用いる場合、柑橘類の抽出物や、柑橘類の酵素処理物などを用いることもできる。また、市販品を用いることもできる。例えば、「酵素処理温州ミカン」（株式会社ダイセル）、「クリプトベータ」（アークレイ株式会社）等が好ましく例示できる。 The composition included in the present invention (for example, the composition described in Items 1 to 4 above) can be used for intestinal improvement. The composition includes β-cryptoxanthin. β-cryptoxanthin is a compound that is abundant in citrus fruits such as mandarin orange and grapefruit, and is particularly abundant in Satsuma mandarin. The β-cryptoxanthin used in the present invention is not particularly limited, and may be derived from, for example, citrus fruits (particularly preferably Citrus unshiu), or may be one extracted from a microorganism or a synthetic product. When using a citrus-derived citrus fruit such as Satsuma mandarin, a citrus extract or a citrus enzyme-treated product can also be used. Commercial products can also be used. For example, “enzyme-treated mandarin orange” (Daicel Co., Ltd.), “crypto beta” (Arkray Co., Ltd.) and the like can be preferably exemplified.
組成物におけるβ−クリプトキサンチンの含有量は、本発明の効果が損なわれない限り特に制限されないが、例えば０．０１〜１００重量％が好ましく、０．１〜９９重量％がさらに好ましい。 The content of β-cryptoxanthin in the composition is not particularly limited as long as the effect of the present invention is not impaired, but is preferably 0.01 to 100% by weight, and more preferably 0.1 to 99% by weight.
本発明に係る組成物の摂取量は、本発明の効果が得られる範囲であれば特に制限されない。例えば、含有されるβ−クリプトキサンチン量が、成人一日あたり、５μｇ〜６４．８ｍｇ程度が好ましく、１０μｇ〜１０ｍｇ程度がより好ましく、１５μｇ〜１ｍｇ程度がさらに好ましく、２０μｇ〜５００μｇ程度がよりさらに好ましく、２５μｇ〜１００μｇ程度が特に好ましい。
最も好ましい。
The intake of the composition according to the present invention is not particularly limited as long as the effects of the present invention are obtained. For example, the amount of β-cryptoxanthin contained is preferably about 5 μg to 64.8 mg per adult day, more preferably about 10 μg to 10 mg, further preferably about 15 μg to 1 mg, and still more preferably about 20 μg to 500 μg. About 25 μg to 100 μg is particularly preferable.
Most preferred.
また、本発明に係る組成物は、１回又は複数回（好ましくは２〜３回）に分けて摂取することができる。適用対象はヒトが好ましいが、ヒト以外の非ヒト哺乳動物であってもよい。適用対象が非ヒト哺乳動物（例えばペット又は家畜、より具体的には、イヌ、ネコ、ウシ、ブタ、ニワトリ、ヒツジ等）の場合も、当該ヒトの投与又は摂取量を参考として適宜設定することができる。また、本発明に係る組成物の生体への適用方法は、腸に適用できれば特に制限されないが、経口又は経腸適用（つまり、経口組成物又は経腸組成物）であることが好ましく、経口で適用することがより好ましい。 In addition, the composition according to the present invention can be taken in one or a plurality of times (preferably 2 to 3 times). The subject of application is preferably a human, but may be a non-human mammal other than a human. Even when the subject of application is a non-human mammal (eg, a pet or livestock, more specifically, a dog, cat, cow, pig, chicken, sheep, etc.), the administration or intake amount of the human should be set as appropriate. Can do. Further, the method of applying the composition according to the present invention to a living body is not particularly limited as long as it can be applied to the intestine. However, oral or enteral application (that is, oral composition or enteral composition) is preferable. It is more preferable to apply.
本発明に係る組成物を用いることで、上述の通り、健常者はもちろん腸内環境が優れない者であっても腸内環境を改善することができる。より具体的には、例えば、「おなかの調子を整える」、「腸内環境を改善する／整える」、「腸内環境を良好にする／良好に保つ」、「腸の調子を改善する／整える」、「腸内フローラ（腸内細菌叢）の菌のバランスを改善する／整える」、「腸内フローラ（腸内細菌叢）を良好にする／良好に保つ」、などの効果を期待して使用することが出来る。 By using the composition according to the present invention, as described above, the intestinal environment can be improved even by healthy persons as well as those who are not excellent in the intestinal environment. More specifically, for example, “condition the tummy”, “improve / condition the intestinal environment”, “make the intestinal environment good / maintain well”, “improve / condition the intestinal condition” ”,“ Improve / adjust the balance of bacteria in the intestinal flora (intestinal flora) ”,“ I want to improve / keep the intestinal flora (intestinal flora) good ”, etc. Can be used.
特に、本発明に係る組成物は、腸内フローラにおけるプレボテラ属の菌の比率を高める又は調整することができる。このため、プレボテラ属の菌の比率が比較的低い者（例えば、外食する機会が多い人、インスタント食品を食べる機会の多い人、脂肪分や動物性タンパクの多い食事を好む人、高脂肪及び高タンパク質の食事が気になる人、野菜や根菜など繊維質を多く含む食品が嫌いな人や食べる機会の少ない人）に特に好適である。 In particular, the composition according to the present invention can increase or adjust the proportion of Prevotella spp. In the intestinal flora. For this reason, people with a relatively low proportion of Prevotella spp. (For example, people who have a lot of opportunities to eat out, people who have a lot of opportunities to eat instant foods, people who prefer a diet high in fat and animal protein, high fat and high It is particularly suitable for those who are worried about protein diet, those who do not like foods containing a lot of fiber such as vegetables and root vegetables, and those who have few opportunities to eat.
本発明に係る組成物は、β−クリプトキサンチンを含み、さらに他の成分を含むことができる。 The composition according to the present invention contains β-cryptoxanthin and may further contain other components.
例えば、上述したように、β−クリプトキサンチンとして柑橘類の抽出物や、柑橘類の酵素処理物などを用いる場合には、これらに含まれるβ−クリプトキサンチン以外の成分を当該他の成分として含むことができる。 For example, as described above, when a citrus extract or a citrus enzyme-treated product is used as β-cryptoxanthin, a component other than β-cryptoxanthin contained therein may be included as the other component. it can.
また、該他の成分として、当該組成物を用いる分野に応じて適宜公知の成分を選択して用いることもできる。例えば、薬学的又は食品衛生学的に許容される担体を用いることができる。 Moreover, as this other component, a well-known component can also be suitably selected and used according to the field | area which uses the said composition. For example, a pharmaceutically or food hygienically acceptable carrier can be used.
本発明に係る組成物は、例えば医薬組成物、食品組成物（飲料組成物及び食品添加物組成物を包含する）として好ましく用いることができる。 The composition according to the present invention can be preferably used as, for example, a pharmaceutical composition or a food composition (including a beverage composition and a food additive composition).
医薬組成物として用いる場合、他の成分としては、薬学的に許容される基剤、担体、及び／又は添加剤（例えば溶剤、分散剤、乳化剤、緩衝剤、安定剤、賦形剤、結合剤、崩壊剤、滑沢剤等）等が例示できる。また、当該医薬組成物の形態も特に制限されず、錠剤、タブレット剤、口中崩壊剤、丸剤、散剤、液剤、顆粒剤、カプセル剤等が例示できる。これらの形態の医薬製剤は、必要に応じて当該他の成分と、β−クリプトキサンチンを組み合わせて常法により調製することができる。 When used as a pharmaceutical composition, other components include pharmaceutically acceptable bases, carriers, and / or additives (eg, solvents, dispersants, emulsifiers, buffers, stabilizers, excipients, binders). , Disintegrants, lubricants, etc.). The form of the pharmaceutical composition is not particularly limited, and examples thereof include tablets, tablets, disintegrants in the mouth, pills, powders, liquids, granules, capsules and the like. These forms of pharmaceutical preparations can be prepared by a conventional method by combining the other components and β-cryptoxanthin as necessary.
食品組成物として用いる場合、他の成分としては、食品衛生学上許容される基剤、担体、添加剤や、その他食品として利用され得る成分・材料が例示できる。また、当該食品組成物の形態も特に制限されず、例えば加工食品、健康食品（栄養補助食品、栄養機能食品、病者用食品、特定保健用食品、機能性表示商品等）、サプリメント、病者向け食品（病院食、病人食又は介護食等）等が例示できる。これらは常法により調製することができる。特に、健康食品（栄養補助食品、栄養機能食品、病者用食品、特定保健用食品、機能性表示食品等）、又はサプリメントとして、食品組成物を調製する場合は、継続的な摂取が行いやすいように、例えば顆粒、カプセル（ソフトカプセル、ハードカプセル、マルチカプセルなど）、タブレット、錠剤（チュアブル剤等を含む）、ゼリー（易崩壊ゲル／ゼリーを含む）、飲料（粉末／顆粒飲料、タブレット飲料、ドリンク剤等）等の形態で調製することが好ましく、なかでもカプセル、タブレット、錠剤、ゼリー、粉末／顆粒飲料、タブレット飲料、ドリンク剤の形態が摂取の簡便さの点からは好ましいが、特にこれらに限定されるものではない。なお、食品組成物の中でも食品添加物組成物として用いる場合には、その形態として、例えば液状、粉末状、フレーク状、顆粒状、ペースト状のものが挙げられる。より具体的には、調味料（醤油、ソース、ケチャップ、ドレッシング等）、フレーク（ふりかけ）、焼き肉のたれ、スパイス、ルーペースト（カレールーペースト等）等が例示できる。 When used as a food composition, examples of other components include bases, carriers, additives, and other components and materials that can be used as food. Also, the form of the food composition is not particularly limited. For example, processed foods, health foods (dietary supplements, nutritional functional foods, foods for the sick, foods for specified health, functional indication products, etc.), supplements, sick people Foods for hospitals (hospital foods, sick foods, nursing foods, etc.) can be exemplified. These can be prepared by conventional methods. In particular, when preparing food compositions as health foods (dietary supplements, nutritional functional foods, foods for the sick, foods for specified health use, functional labeling foods, etc.) or supplements, continuous intake is easy to perform. For example, granules, capsules (soft capsules, hard capsules, multi-capsules, etc.), tablets, tablets (including chewables etc.), jelly (including easily disintegrating gel / jelly), beverages (powder / granular beverages, tablet beverages, drinks) In particular, capsules, tablets, tablets, jelly, powder / granular drinks, tablet drinks, and drinks are preferable in terms of ease of ingestion. It is not limited. When used as a food additive composition among food compositions, examples of the form include liquid, powder, flake, granule, and paste. More specifically, seasonings (soy sauce, sauce, ketchup, dressing, etc.), flakes (sprinkles), grilled meat sauce, spices, roux paste (carrere paste, etc.) and the like can be exemplified.
なお、上述の通り、（ｉ）脂肪分や動物性タンパクの多い食事を摂る人と、（ｉｉ）野菜中心で炭水化物の多い食事を摂る人とでは、（ｉ）の人では腸内フローラにおいてバクテロイデス（Ｂａｃｔｅｒｏｉｄｅｓ）属の比率が高く、一方（ｉｉ）の人では腸内フローラにおいてプレボテラ（Ｐｒｅｖｏｔｅｌｌａ）属の比率が高いことから、プレボテラ属の菌の腸内フローラ占有率を高めるためには、野菜や炭水化物を多く摂取することも有効であると考えられる。よって、本発明の組成物においては、他の成分として、野菜や炭水化物を含むことも好適であり、特に食品組成物において野菜や米、麺類などの炭水化物を多く他の成分として含むことは好ましい。例えば、食品組成物がゼリーや飲料である場合には、本発明の食品組成物がβ−クリプトキサンチンを含む野菜ゼリーや野菜ジュースであることが中でも好適である。また例えば、β−クリプトキサンチンを含む野菜ピューレ及び当該ピューレを用いた食品も好適である。 In addition, as described above, (i) a person who eats a diet high in fat and animal protein, and (ii) a person who eats a diet high in carbohydrates mainly in vegetables, the person in (i) has a Bacteroides in the intestinal flora. Since the ratio of the genus (Bacteroides) is high and the ratio of the genus Prevoterra in the intestinal flora is high in the person of (ii), in order to increase the intestinal flora occupancy rate of the bacteria of the genus Prebotella, It is considered effective to consume a lot of carbohydrates. Therefore, in the composition of this invention, it is also suitable to contain vegetables and carbohydrates as another component, and it is preferable to contain many carbohydrates, such as vegetables, rice, and noodles, as other components especially in a food composition. For example, when the food composition is a jelly or a beverage, it is particularly preferable that the food composition of the present invention is a vegetable jelly or vegetable juice containing β-cryptoxanthin. For example, vegetable purees containing β-cryptoxanthin and foods using the purees are also suitable.
なお、本明細書において「含む」とは、「本質的にからなる」と、「からなる」をも包含する（Ｔｈｅ ｔｅｒｍ “ｃｏｍｐｒｉｓｉｎｇ” ｉｎｃｌｕｄｅｓ “ｃｏｎｓｉｓｔｉｎｇ ｅｓｓｅｎｔｉａｌｌｙ ｏｆ” ａｎｄ “ｃｏｎｓｉｓｔｉｎｇ ｏｆ．”）。 In this specification, “comprising” includes “consisting essentially of” and “consisting of” (The term “comprising” includes “consisting essentially of” and “consisting of.”).
以下、本発明を具体的に説明するが、本発明は下記の例に限定されるものではない。 Hereinafter, the present invention will be specifically described, but the present invention is not limited to the following examples.
＜使用動物及び群分け＞
動物種：マウス（ＳＰＦ）
系統：Ｃ５７ＢＬ／６Ｊ 日本チャールス・リバー株式会社
雄、４週齢
<Animals used and grouping>
Animal species: Mouse (SPF)
System: C57BL / 6J Nippon Charles River Co., Ltd. Male, 4 weeks old
マウスを購入後、５日間の検疫期間、その後２日間の馴化期間を設け、体重推移及び一般状態に異常の認められないものを群分けに用いた。群分けはコンピュータシステム（ＩＢＵＫＩ、株式会社日本バイオリサーチセンター）を用いて、体重を層別に分けたのち、無作為抽出法により各群の平均体重がほぼ等しくなるように馴化終了日に行った。 After the mice were purchased, a quarantine period of 5 days and a acclimation period of 2 days were provided, and mice with no abnormalities in weight transition and general condition were used for grouping. Grouping was carried out on the acclimatization end date by using a computer system (IBUKI, Japan Bioresearch Center Co., Ltd.), dividing the body weight into layers, and using a random sampling method so that the average body weight of each group was almost equal.
具体的には、表１に記載の５群にｎ＝５づつ振り分けた。 Specifically, n = 5 were assigned to 5 groups shown in Table 1.
＜被験物質＞
抗肥満効果のある素材として知られる、あしたばを対象に用いて、βクリプトキサンチンの効果を検討した。
<Test substance>
The effect of β-cryptoxanthin was examined using Ashitaba, which is known as an anti-obesity material.
被検物質はオリーブ油（和光純薬工業株式会社）を用いて４０ｍｇ／ｍＬまたは１６０ｍｇ／ｍＬとなるように溶解し、ゾンデを用いて経口で投与した。一日あたりの被験物質投与量を表１に示す。 The test substance was dissolved in olive oil (Wako Pure Chemical Industries, Ltd.) to 40 mg / mL or 160 mg / mL and administered orally using a sonde. Table 1 shows the dose of the test substance per day.
用いた被験物質の詳細は次の通りである。
・あした葉ポリフェノール（ＣＡＬＳＡＰ）： 株式会社日本生物化学研究所
・酵素処理温州ミカン β−クリプトキサンチン０．１５％含有：株式会社ダイセル
The details of the test substance used are as follows.
・ Tomorrow leaf polyphenol (CALSAP): Nippon Biochemical Laboratories Co., Ltd. ・ Enzyme-treated Unshu mandarin β-cryptoxanthin 0.15% contained: Daicel Corporation
＜飼料及び食餌条件＞
普通食飼料：ＣＲＦ−１、オリエンタル酵母工業株式会社
高脂肪食飼料：ＨＦＤ−６０、オリエンタル酵母工業株式会社
検疫・馴化期間及び群分け後の普通食群はＣＲＦ−１を給餌器に入れて自由に摂取させた。群分け後のその他の群はＨＦＤ−６０を給餌器に入れ、自由に摂取させた。
群分け前日から当日、群分け後（すなわち試験開始から）２５日〜２６日、２６日〜２７日、及び投与２７日〜２８日に、各個体のケージ中の糞便を採取した。糞便を入れたチューブは超低温フリーザー中で凍結保存した。
<Feed and diet conditions>
Normal diet feed: CRF-1, Oriental Yeast Co., Ltd. High fat diet feed: HFD-60, Oriental Yeast Industry Co., Ltd. Quarantine / acclimation period and regular diet group after grouping are free to put CRF-1 in the feeder Ingested. The other groups after grouping placed HFD-60 in a feeder and allowed to eat freely.
Feces in the cage of each individual were collected from the day before the grouping, the day after grouping (ie, from the start of the test) from 25 to 26 days, 26 to 27 days, and 27 to 28 days after administration. The tube containing feces was stored frozen in an ultra-low temperature freezer.
＜菌叢解析＞
糞便中の菌叢解析については、各検体の細菌叢に由来する１６ＳｒＤＮＡ部分塩基配列のＴ−ＲＦＬＰ（Ｔｅｒｍｉｎａｌ Ｒｅｓｔｒｉｃｔｉｏｎ Ｆｒａｇｍｅｎｔ Ｌｅｎｇｔｈ Ｐｏｌｙｍｏｒｐｈｉｓｍ）解析を行い、得られたデータに基づいて検体中の主要な分類群の簡易的な推定およびクラスター解析による検体間の比較を実施した。なお、当該菌叢解析には、２５日〜２６日、２６日〜２７日、及び投与２７日〜２８日に、採取した糞便を混合したものを検体として用いた。また、解析は株式会社テクノスルガラボに依頼した。
<Bacteria analysis>
For the analysis of faecal flora in feces, T-RFLP (Terminal Restriction Fragment Length Polymorphism) analysis of the 16S rDNA partial nucleotide sequence derived from the bacterial flora of each specimen is performed, and the main taxonomic groups in the specimen based on the obtained data Comparison between specimens was performed by simple estimation and cluster analysis. In addition, in the said microflora analysis, what mixed the extract | collected feces on 25th-26th, 26th-27th, and administration 27th-28th was used as a test substance. Analysis was requested from Techno Suruga Lab.
菌叢解析の結果算出された、プレボテラ属の菌が腸内フローラにおいて占める割合をグラフとして図１に示す。なお、図１では、高脂肪食をＦＦＤと標記する。 FIG. 1 shows the ratio of Prevotella spp. In the intestinal flora calculated as a result of the flora analysis as a graph. In FIG. 1, the high fat diet is denoted as FFD.
あしたば及びβクリプトキサンチンは、いずれも抗肥満素材として知られているが、対照的な結果を示した。すなわち、あしたばを高脂肪食とあわせて摂取した群では、プレボテラ属の菌が腸内フローラにおいて占める割合は改善（増加）するどころか、むしろ高脂肪食のみを摂取した群よりも有意に悪化（減少）した。一方、β−クリプトキサンチンを高脂肪食とあわせて摂取した群では、プレボテラ属の菌が腸内フローラにおいて占める割合は高脂肪食のみを摂取した群に比べて改善（増加）しており、普通食のみを摂取した群と比べても遜色なかった。 Ashitaba and β-cryptoxanthin, both known as anti-obesity materials, showed contrasting results. In other words, in the group that took tomato together with the high-fat diet, the proportion of Prevotella spp. In the intestinal flora improved (increased), but was significantly worse (decreased) than the group that took only the high-fat diet. )did. On the other hand, in the group that ingested β-cryptoxanthin with a high-fat diet, the proportion of Prevotella spp. In the intestinal flora has improved (increased) compared to the group that ingested only a high-fat diet. Compared to the group that took only food, it was inferior.
またさらに、Ｔ−ＲＦＬＰ解析プロファイルに基づき、クラスター解析を実施した結果（デンドログラム）を図２に示す。β−クリプトキサンチンを高脂肪食とあわせて摂取した群と、普通食のみを摂取した群とが、よく似たプロファイルを有することが明らかとなった。なお、クラスター解析には、以下のソフト及び手法を用いた。
解析ソフト：Ｇｅｎｅ Ｍａｔｈｓ（Ａｐｐｌｉｅｄ１Ｍａｔｈｓ、Ｂｅｌｇｉｕｍ）
クラスタリング方法：ＵＰＧＭＡ
距離関数：ピアソンの相関関数
Furthermore, the result (dendrogram) which implemented cluster analysis based on the T-RFLP analysis profile is shown in FIG. It was clarified that the group that ingested β-cryptoxanthin together with the high fat diet and the group that ingested only the normal diet had similar profiles. The following software and method were used for cluster analysis.
Analysis software: Gene Maths (Applied 1 Maths, Belgium)
Clustering method: UPGMA
Distance function: Pearson's correlation function
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001269135A (en) * | 2000-03-28 | 2001-10-02 | Meiji Milk Prod Co Ltd | Nutritional composition capable of promoting body protein accumulation efficiency |
| WO2014208511A1 (en) * | 2013-06-27 | 2014-12-31 | 学校法人同志社 | Intestinal flora improving composition containing astaxanthin |
| JP2015520176A (en) * | 2012-06-06 | 2015-07-16 | シャンハイ ジャオ トン ユニバーシティ | Methods and compositions for improving gut microbiota population |
| JP2016069317A (en) * | 2014-09-29 | 2016-05-09 | 株式会社ユーグレナ | Inhibitors that inhibit progression of renal failure, prophylactics of renal failure, and inhibitors of indoxyl sulfate production |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001269135A (en) * | 2000-03-28 | 2001-10-02 | Meiji Milk Prod Co Ltd | Nutritional composition capable of promoting body protein accumulation efficiency |
| JP2015520176A (en) * | 2012-06-06 | 2015-07-16 | シャンハイ ジャオ トン ユニバーシティ | Methods and compositions for improving gut microbiota population |
| WO2014208511A1 (en) * | 2013-06-27 | 2014-12-31 | 学校法人同志社 | Intestinal flora improving composition containing astaxanthin |
| JP2016069317A (en) * | 2014-09-29 | 2016-05-09 | 株式会社ユーグレナ | Inhibitors that inhibit progression of renal failure, prophylactics of renal failure, and inhibitors of indoxyl sulfate production |
Non-Patent Citations (1)
| Title |
|---|
| "β−クリプトキサンチン高含有ミカンジュースが食餌誘導肥満マウスの炎症応答と盲腸内細菌叢に及ぼす影響の", 日本農芸化学会大会講演要旨集, vol. 2016, JPN6020016219, March 2016 (2016-03-01), pages 3 - 101, ISSN: 0004406415 * |
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