JP2014507146A - B型肝炎ウイルスコアタンパク質をコードする核酸分子およびそれを含むワクチン - Google Patents
B型肝炎ウイルスコアタンパク質をコードする核酸分子およびそれを含むワクチン Download PDFInfo
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Abstract
【選択図】なし
Description
a.アジュバント
b.抗体
c.コード配列
d.補体
e.コンセンサスまたはコンセンサス配列
f.電気穿孔
g.フラグメント
h.遺伝子構築物
i.同一
j.免疫応答
k.核酸
l.動作可能に連結する
m.プロモーター
n.シグナルペプチド
o.ストリンジェントなハイブリダイゼーション条件
p.実質的に相補的
q.実質的に同一
r.亜型または血清型
s.変種
t.ベクター
2.HBVコア抗原
3.遺伝子配列、構築物、およびプラスミド
C>G 241 CMVプロモーター、
C>T 1942 主鎖、ウシ成長ホルモンポリアデニル化シグナル(bGHpolyA)の下流、
A>− 2876 主鎖、カンマイシン遺伝子の下流、
C>T 3277 pUCの複製の起点(複製起点)高コピー数突然変異体(Nucleic Acid Research 1985を参照すること)、
G>C 3753 RNASeH部位の上流のpUC複製起点の最末端、
塩基対2、3、および4は、CMVプロモーターの上流の主鎖においてACTからCTGに変わる。
ベクターの主鎖は、pAV0242であり得る。ベクターは、複製欠損アデノウイルス型5(Ad5)ベクターであり得る。
4.薬学的組成物
5.送達の方法
a.併用治療
b.投与経路
c.電気穿孔
d.ワクチンを調製する方法
Claims (23)
- 配列番号2を含むタンパク質、配列番号2と98%相同であるタンパク質、少なくとも20個のアミノ酸であり、配列番号2を含むタンパク質の免疫原性フラグメント、および少なくとも20個のアミノ酸である配列番号2と98%相同であるタンパク質の免疫原性フラグメントからなる群から選択される1つ以上のタンパク質をコードするコード配列を含む、核酸分子。
- 前記タンパク質のN末端に連結されるシグナルペプチドを更に含む、請求項1に記載の核酸分子。
- 配列番号2、配列番号4、および配列番号6からなる群から選択される1つ以上のタンパク質をコードする、請求項1に記載の核酸分子。
- 配列番号1を含む核酸配列、配列番号1と98%相同である核酸配列、配列番号1によってコードされる、少なくとも20個のアミノ酸を含む免疫原性フラグメントをコードする核酸配列を含むそのフラグメント、および配列番号1によってコードされるタンパク質と98%相同であるタンパク質の少なくとも20個のアミノ酸を含む免疫原性フラグメントをコードする核酸配列を含むそのフラグメントからなる群から選択される1つ以上の配列を含む、請求項1に記載の核酸分子。
- 前記核酸配列の5’末端に連結されるシグナルペプチドを更に含む、請求項1に記載の核酸分子。
- 配列番号1、配列番号3、および配列番号5からなる群から選択される1つ以上のヌクレオチド配列を含む、請求項1に記載の核酸分子。
- 前記核酸分子がプラスミドである、請求項1に記載の核酸分子。
- 前記核酸分子が発現ベクターであり、前記1つ以上のタンパク質をコードする配列が調節要素に動作可能に連結される、請求項1に記載の核酸分子。
- 前記核酸分子がウイルス粒子に組み込まれる、請求項1に記載の核酸分子。
- 配列番号2を含むタンパク質の前記免疫原性フラグメントが、少なくとも60個、少なくとも120個、または少なくとも180個のアミノ酸である、請求項1に記載の核酸分子。
- 配列番号2と98%相同であるタンパク質の前記免疫原性フラグメントが、少なくとも60個、少なくとも120個、または少なくとも180個のアミノ酸である、請求項1に記載の核酸分子。
- 請求項1に記載の核酸分子を個人に投与することを含む、HBV抗原に対する免疫応答を誘導する方法。
- 請求項1に記載の核酸分子を個人に投与することを含む、個人をHBV感染から保護する方法。
- 請求項1に記載の核酸分子を個人に投与することを含む、HBV感染と診断された個人を保護する方法。
- 配列番号2、
配列番号2と98%相同であるタンパク質、
配列番号2の20個以上のアミノ酸を含む配列番号2の免疫原性フラグメント、
20個以上のアミノ酸を含む配列番号2と98%相同であるタンパク質の免疫原性フラグメント、
からなる群から選択される、タンパク質。 - 配列番号2を含むタンパク質の前記免疫原性フラグメントが、少なくとも60個、少なくとも120個、または少なくとも180個のアミノ酸である、請求項15に記載のタンパク質。
- 配列番号2と98%相同であるタンパク質の前記免疫原性フラグメントが、少なくとも60個、少なくとも120個、または少なくとも180個のアミノ酸である、請求項15に記載のタンパク質。
- 配列番号2、配列番号4、および配列番号6からなる群から選択されるタンパク質をコードする、請求項15に記載のタンパク質。
- 請求項15に記載の核酸分子を個人に投与することを含む、HBV抗原に対する免疫応答を誘導する方法。
- 請求項15に記載の核酸分子を個人に投与することを含む、個人をHBV感染から保護する方法。
- 請求項15に記載の核酸分子を個人に投与することを含む、HBV感染と診断された個人を保護する方法。
- 請求項1に記載の核酸分子と、
アジュバント分子と、
を含む、対象におけるHBVに対する免疫応答の生成に有用なワクチン。 - 前記アジュバントが、IL−12、IL−15、IL−28、またはランテスである、請求項22に記載のワクチン。
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US9238679B2 (en) | 2011-02-11 | 2016-01-19 | The Trustees Of The University Of Pennslyvania | Nucleic acid molecule encoding hepatitis B virus core protein and surface antigen protein and vaccine comprising the same |
MX343830B (es) | 2011-02-11 | 2016-11-24 | Univ Pennsylvania | Molecula de acido nucleico que codifica la proteina nuclear del virus de la hepatitis b y vacuna que comprende la misma. |
EP3222677A1 (de) * | 2016-03-23 | 2017-09-27 | LANXESS Deutschland GmbH | Metallazopigmente |
WO2017180770A1 (en) | 2016-04-13 | 2017-10-19 | Synthetic Genomics, Inc. | Recombinant arterivirus replicon systems and uses thereof |
CN110073002B (zh) | 2016-10-17 | 2024-06-11 | 杨森制药公司 | 重组病毒复制子体系及其用途 |
KR102655641B1 (ko) | 2016-12-05 | 2024-04-05 | 얀센 파마슈티칼즈, 인코포레이티드 | 유전자 발현을 향상시키기 위한 조성물 및 방법 |
EA202091517A1 (ru) | 2017-12-19 | 2020-11-03 | Янссен Сайенсиз Айрлэнд Анлимитед Компани | Способы и устройство для доставки вакцин против вируса гепатита b (hbv) |
EA202091513A1 (ru) | 2017-12-19 | 2020-09-09 | Янссен Сайенсиз Айрлэнд Анлимитед Компани | Вакцины против вируса гепатита b (hbv) и их применение |
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