JP2014111652A - Methioninase inhibitor and composition for oral cavity and food and drink containing the same - Google Patents
Methioninase inhibitor and composition for oral cavity and food and drink containing the same Download PDFInfo
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- JP2014111652A JP2014111652A JP2014039776A JP2014039776A JP2014111652A JP 2014111652 A JP2014111652 A JP 2014111652A JP 2014039776 A JP2014039776 A JP 2014039776A JP 2014039776 A JP2014039776 A JP 2014039776A JP 2014111652 A JP2014111652 A JP 2014111652A
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Abstract
Description
本発明は、口臭の原因物質であるメチルメルカプタンの産生に関与するメチオニナーゼ阻害剤及びそれを含有する口腔用組成物並びに飲食品に関する。 The present invention relates to a methioninase inhibitor involved in the production of methyl mercaptan which is a causative substance of bad breath, an oral composition containing it, and a food and drink.
口臭は、口腔より出される悪臭の総称である。口臭強度と相関性のある成分は硫化水素、メチルメルカプタン及びジメチルスルフィド等の揮発性硫黄化合物であり、中でもメチルメルカプタンは口臭と強い相関関係が認められる。これらは食物残渣、口腔内の剥離上皮細胞及び唾液タンパク質等を口腔内細菌が代謝、分解することにより発生する。メチルメルカプタンをはじめとする揮発性硫黄化合物は、口腔内での蛋白質合成の抑制、コラーゲン合成の抑制及び口腔内粘膜の透過性の亢進により人体に対して毒性が高いことが知られている。従ってメチルメルカプタンは単に口臭の原因物質であるだけではなく、体内環境、特に口腔疾患の悪化の一端を担っており、その低減化は重要な課題である。 Bad breath is a general term for bad odors emitted from the oral cavity. Components that correlate with bad breath intensity are volatile sulfur compounds such as hydrogen sulfide, methyl mercaptan, and dimethyl sulfide, and methyl mercaptan has a strong correlation with bad breath. These occur when oral bacteria metabolize and decompose food residues, exfoliated epithelial cells in the oral cavity, salivary proteins, and the like. It is known that volatile sulfur compounds such as methyl mercaptan are highly toxic to the human body due to suppression of protein synthesis in the oral cavity, suppression of collagen synthesis, and increased permeability of the oral mucosa. Therefore, methyl mercaptan is not only a causative substance of bad breath, but also plays a part in the deterioration of the body environment, particularly oral diseases, and its reduction is an important issue.
従来の口臭除去製品として歯磨き剤、洗口剤、マウススプレーに代表される口中清涼剤、ガム、キャンディ及びタブレット等の口中清涼菓子が提案されている。これらの製品の中には口臭除去の目的のために悪臭成分に対する直接的な消臭素材が配合されていることが多い。メチルメルカプタンに化学的に反応する消臭素材としては緑茶（例えば、特許文献１参照。）、延命草（例えば、特許文献２参照。）及びラズベリー（例えば、特許文献３参照。）等、多数報告されている。また、抗菌剤を配合している製品も数多く存在する。 Mouth refreshing products such as toothpastes, mouth washes, mouth refreshing agents such as mouth sprays, gums, candy, and tablets have been proposed as conventional products for removing bad breath. These products often contain a direct deodorizing material for malodorous components for the purpose of removing bad breath. As a deodorizing material that chemically reacts with methyl mercaptan, many reports such as green tea (for example, see Patent Document 1), life-prolonging grass (for example, see Patent Document 2), and raspberry (for example, see Patent Document 3) are reported. Has been. There are also many products that contain antibacterial agents.
口臭の主原因であるメチルメルカプタンは、口腔内のタンパク質分解産物であるＬ−メチオニンを基質として、フゾバクテリウム・ヌクレイタム（Ｆｕｓｏｂａｃｔｅｒｉｕｍ ｎｕｃｌｅａｔｕｍ）及びポルフィロモナス・ジンジバリス（Ｐｏｒｐｈｙｒｏｍｏｎａｓ ｇｉｎｇｉｖａｌｉｓ）等の口腔内細菌の菌体内酵素のメチオニナーゼ（Ｌ−メチオニンγリアーゼ）により産生される。近年、このメチオニナーゼを阻害することによる口臭予防の試みがなされている。メチオニナーゼを阻害することは、メチルメルカプタンの産生そのものを抑制することから非常に効果的である。また、メチオニナーゼは人体内には存在しないことからメチオニナーゼを阻害することは生体に影響を与えない。このことからメチルメルカプタン発生のカギとなる口腔内細菌由来のメチオニナーゼを阻害することにより、効果的かつ安全な口臭抑制が可能である。 Methyl mercaptan, which is the main cause of bad breath, is the body of oral bacteria such as Fusobacterium nucleatum and Porphyromonas gingivalis, using L-methionine, a proteolytic product in the oral cavity, as a substrate. Produced by the enzyme methioninase (L-methionine gamma lyase). In recent years, attempts have been made to prevent bad breath by inhibiting this methioninase. Inhibiting methioninase is very effective because it suppresses the production of methyl mercaptan itself. Further, since methioninase does not exist in the human body, inhibiting methioninase does not affect the living body. For this reason, it is possible to effectively and safely suppress bad breath by inhibiting methioninase derived from oral bacteria, which is the key to generation of methyl mercaptan.
現在まで、メチオナーゼの阻害剤としてトマト抽出物、アセンヤク抽出物及びキラヤ樹皮抽出物等、天然物由来のものが報告されており（例えば、特許文献４参照。）、これらが口腔内細菌ポルフィロモナス・ジンジバリスのメチルメルカプタン産生を抑制することが明らかになっている。また、キク科のキク属、チョウセンアザミ属及びマンジュギク属植物（例えば、特許文献５参照。）及び植物精油成分（例えば、非特許文献１参照。）が口腔内細菌フゾバクテリウム・ヌクレイタムのメチルメルカプタン産生抑制効果を示すことが、特定の香料成分（例えば、特許文献６、７参照。）及びケト酸とその塩類（例えば、特許文献８参照。）が口腔内細菌のメチルメルカプタン産生を抑制することが報告されている。 To date, natural substances such as tomato extract, asenyaku extract, and quilla bark extract have been reported as inhibitors of methionase (see, for example, Patent Document 4), which are oral bacteria Porphyromonas. -It has been shown that gingivalis suppresses methyl mercaptan production. In addition, plants belonging to the genus Chrysanthemum, Chrysanthemum genus and Manjugiku (for example, see Patent Document 5) and plant essential oil components (for example, see Non-Patent Document 1) inhibit methylmercaptan production of oral bacteria Fusobacterium nucleatum. It is reported that specific perfume ingredients (for example, see Patent Documents 6 and 7) and keto acids and salts thereof (for example, see Patent Document 8) suppress the production of methyl mercaptan by oral bacteria. Has been.
また、クワ葉を有効成分とする口腔用組成物及びグァバを含有する液体う蝕予防剤組成物の歯垢形成酵素であるグルコシルトランスフェラーゼに対する阻害効果（例えば、特許文献９、１０参照。）や、グァバ葉抽出エキスを有効成分とする歯周病防止剤が開示されている（例えば、特許文献１１参照。）が、メチオニナーゼ阻害効果は明らかにされてはいない。ホアハウンドについては硫酸還元菌の硫化水素産生を抑制することが明らかになっている（例えば、特許文献１２参照。）が、メチオニナーゼの阻害効果は明らかになっていない。オオバコについてはプロテアーゼ阻害効果が明らかとなっている（例えば、特許文献１３参照）が、メチオニナーゼ阻害効果については明らかになっていない。フェンネルについてはプロテアーゼ阻害剤としてポルフィロモナス・ジンジバリスの粗酵素の阻害効果が明示されている（例えば、特許文献１３参照。）が、メチオニナーゼ阻害効果については触れられていない。 Moreover, the inhibitory effect (for example, refer patent document 9, 10) with respect to the glucosyltransferase which is a plaque forming enzyme of the composition for oral cavity which uses mulberry leaves as an active ingredient, and the liquid caries preventive composition containing guava, A periodontal disease inhibitor containing guava leaf extract as an active ingredient is disclosed (for example, see Patent Document 11), but the methioninase inhibitory effect has not been clarified. For Hoehound, it has been clarified that hydrogen sulfide production by sulfate-reducing bacteria is suppressed (see, for example, Patent Document 12), but the inhibitory effect of methioninase has not been clarified. For psyllium, the protease inhibitory effect has been clarified (see, for example, Patent Document 13), but the methioninase inhibitory effect has not been clarified. Regarding fennel, the inhibitory effect of the crude enzyme of Porphyromonas gingivalis as a protease inhibitor is clearly shown (for example, see Patent Document 13), but the methioninase inhibitory effect is not mentioned.
さらに、シソについては口腔組成物として虫歯の原因菌であるミュータンス菌のグルコシルトランスフェラーゼ阻害効果が明示されている（例えば、特許文献１４参照。）が、メチオニナーゼ阻害効果については触れられていない。緑茶についてはでポルフィロモナス・ジンジバリスのプロテアーゼ阻害効果は認められるが、メチオナーゼ阻害効果はないことが記載されている（例えば、特許文献４参照。）。 Furthermore, with regard to perilla, the glucosyltransferase inhibitory effect of mutans bacteria, which is a causative bacterium of dental caries, is clearly shown as an oral composition (for example, see Patent Document 14), but the methioninase inhibitory effect is not mentioned. Regarding green tea, it is described that the protease inhibitory effect of Porphyromonas gingivalis is recognized, but there is no methionase inhibitory effect (see, for example, Patent Document 4).
本発明の目的は、人体に影響がなく安心して使用できる安全性の高い天然植物抽出物を有効成分とする、細菌由来のメチオニナーゼを阻害することによりメチルメルカプタンの産生抑制作用を有する阻害剤及びそれを含有する口腔用組成物並びに飲食品を提供することである。 An object of the present invention is to use a highly safe natural plant extract that does not affect the human body and can be used with confidence as an active ingredient, and an inhibitor having an inhibitory effect on methyl mercaptan production by inhibiting bacterial methioninase and the same It is providing the composition for oral cavity containing foodstuffs, and food-drinks.
本発明者らは、上記課題を解決するために、副作用がなく安全性が高く古来より利用されている生薬及びハーブ等の天然物抽出物に注目し、高いメチオニナーゼ活性を有する口臭原因菌の一つであるフゾバクテリウム・ヌクレイタムＡＴＣＣ２５５８６由来の菌破砕液を用いてその阻害試験を実施した結果、アカメガシワ（Ｍａｌｌｏｔｕｓ ｊａｐｏｎｉｃｕｓ）、クワ（Ｍｏｒｕｓ ａｌｂａ）、アイスランドゴケ（Ｃｅｔｒａｒｉａ ｉｓｌａｎｄｉｃａ）、グァバ（Ｐｓｉｄｉｕｍ ｇｕａｊａｖａ）、ホアハウンド（Ｍａｒｒｕｂｉｕｍ ｖｕｌｇａｒｅ）、ガラナ（Ｐａｕｌｌｉｎｉａ ｃｕｐａｎａ）、オオバコ（Ｐｌａｎｔａｇｏ ａｓｉａｔｉｃａ）、アルカネット（Ａｎｃｈｕｓａ ｏｆｆｉｃｉｎａｌｉｓ）、アケビ（Ａｋｅｂｉａ ｑｕｉｎａｔａ）、クコ（Ｌｙｃｉｕｍ ｃｈｉｎｅｎｓｅ）、カゴソウ（Ｐｒｕｎｅｌｌａ ｖｕｌｇａｒｉｓ）、オレガノ（Ｏｒｉｇａｎｕｍ ｖｕｌｇａｒｅ）、フェンネル（Ｆｏｅｎｉｃｕｌｕｍ ｖｕｌｇａｒｅ）、ハス（Ｎｅｌｕｍｂｏ ｎｕｃｉｆｅｒａ）、キンカン（Ｆｏｒｔｕｎｅｌｌａ ｊａｐｏｎｉｃａ）、緑茶（Ｃａｍｅｌｌｉａ ｓｉｎｅｎｓｉｓ）及びシソ（Ｐｅｒｉｌｌａ ｆｒｕｔｅｓｃｅｎｓ）より得られる抽出物が、メチオニナーゼ阻害活性を有することを見出し、本発明品を完成させた。 In order to solve the above-mentioned problems, the present inventors have focused on natural product extracts such as herbal medicines and herbs that have no side effects and are highly safe and have been used since ancient times. One of the bad breath-causing bacteria having high methioninase activity. As a result of carrying out the inhibition test using the lysate derived from Fusobacterium nucleatum ATCC25586, the results showed that the plant was found to be a mulberry, Morus alba, Icelandic moss, Psidium guaja, Psidium guaja (Marrubium vulgare), Guarana (Paulinia cupana), Psyllium (Plantago asiatica), Alkanet (Anchusa officinalis), Akebei (Ak) ebia quinata), wolfberry (Prunella vulgaris), oregano (Orangeum vulgare), fennel (Fenicum vulgare), lotus (Nelumbo nuci) ) Was found to have methioninase inhibitory activity, and the product of the present invention was completed.
即ち、本発明は、上記植物から選択される１種の植物抽出物を有効成分とすることを特徴とするメチオニナーゼ阻害剤及びそれを含有することを特徴とする口腔用組成物並びに飲食品である。 That is, the present invention is a methioninase inhibitor characterized by comprising one plant extract selected from the above-mentioned plants as an active ingredient, and an oral composition and a food or drink comprising the same. .
本発明のメチオニナーゼ阻害剤は、メチルメルカプタンの産生を抑制する作用を有する。従って、これらを医薬品として、また飲食品に添加して経口的に摂取したり、口中清涼剤、歯磨き粉等の口腔用組成物として口腔内で使用することにより、口臭の減少及び口腔内環境の改善が可能である。 The methioninase inhibitor of the present invention has an action of suppressing the production of methyl mercaptan. Therefore, these can be taken orally by adding them to medicines and foods and used in the oral cavity as oral compositions such as mouth fresheners and toothpastes, thereby reducing bad breath and improving the oral environment. Is possible.
また、本発明品で使用する植物は、いずれも食品素材、ハーブティ及び天然添加物として古くより用いられているものであり、その安全性については全く問題ない。 In addition, the plants used in the present invention are all used as food materials, herbal teas and natural additives for a long time, and there is no problem with respect to their safety.
本発明品で使用する植物、カラホオ（Ｍａｇｎｏｌｉａ ｏｆｆｉｃｉｎａｌｉｓ）、ホオノキ（Ｍａｇｎｏｌｉａ ｏｂｏｖａｔａ）、アカメガシワ（Ｍａｌｌｏｔｕｓ ｊａｐｏｎｉｃｕｓ）、クワ（Ｍｏｒｕｓ ａｌｂａ）、アイスランドゴケ（Ｃｅｔｒａｒｉａ ｉｓｌａｎｄｉｃａ）、グァバ（Ｐｓｉｄｉｕｍ ｇｕａｊａｖａ）、ホアハウンド（Ｍａｒｒｕｂｉｕｍ ｖｕｌｇａｒｅ）、ガラナ（Ｐａｕｌｌｉｎｉａ ｃｕｐａｎａ）、オオバコ（Ｐｌａｎｔａｇｏ ａｓｉａｔｉｃａ）、アルカネット（Ａｎｃｈｕｓａ ｏｆｆｉｃｉｎａｌｉｓ）、アケビ（Ａｋｅｂｉａ ｑｕｉｎａｔａ）、クコ（Ｌｙｃｉｕｍ ｃｈｉｎｅｎｓｅ）、カゴソウ（Ｐｒｕｎｅｌｌａ ｖｕｌｇａｒｉｓ）、オレガノ（Ｏｒｉｇａｎｕｍ ｖｕｌｇａｒｅ）、フェンネル（Ｆｏｅｎｉｃｕｌｕｍ ｖｕｌｇａｒｅ）、ハス（Ｎｅｌｕｍｂｏ ｎｕｃｉｆｅｒａ）、キンカン（Ｆｏｒｔｕｎｅｌｌａ ｊａｐｏｎｉｃａ）、緑茶（Ｃａｍｅｌｌｉａ ｓｉｎｅｎｓｉｓ）及びシソ（Ｐｅｒｉｌｌａ ｆｒｕｔｅｓｃｅｎｓ）は、その全体、葉、果実、花、樹皮、根等の部位を使用することができるが、オレガノ、クワ、クコ、シソ及びハスについてはその葉を、アカメガシワについてはその樹皮を、アルカネットについてはその根及び根茎を、アケビ、ガラナ、キンカン及びフェンネルについてはその果肉、果実を、オオバコはその種子を、カゴソウ及びグァバは、好ましくはその花、カラホオ及びホオノキについてはその樹皮、果実を、アイスランドゴケ及びホアハウンドについてはその全草を、緑茶はその葉を使用することが望ましい。 Plants used in the present invention, calahoo (Magnolia officinalis), honoki (Magnolia obobata), red mulberry (Mallouta muradua), mulberry (Morusu varu) ), Guarana (Paulinia cupana), psyllium (Plantago asiatica), alkanet (Anchusa officinalis), akebi (Akebia quinata), wolfberry (O), gul (v), gal e), fennel (Forenicum vulgare), lotus (Nelumbo nucifera), kumquat (Fortunella japonica), green tea (Camellia sinensis) and perilla (Perilla frutescens), its parts, leaves, fruit, Can be used, but for oregano, mulberry, wolfberry, perilla and lotus, its bark for red wrinkles, its roots and rhizomes for alcanet, and its flesh for akebi, guarana, kumquat and fennel Fruit, psyllium, its seeds, kasou and guava, preferably its flowers, calaho and honoki bark, fruit, iceland moss and hoahound its whole plant, It is desirable to use the green tea leaves.
本発明における植物抽出物を得るには、従来公知の抽出方法によって作製することができ特に限定はしないが、一般的には最初に上記植物を適当な手段で粉砕する。次に、水、メタノール、エタノール、ｎ−プロパノール並びにｎ−ブタノール等の低級アルコール、エーテル、クロロホルム、酢酸エチル、アセトン、グリセリン、プロピレングリコール等の有機溶剤の１種または２種以上の混合溶媒を加えて、従来行なわれている抽出方法によって抽出する。しかし、本発明品は、医薬品、口腔内組成物としてまた飲食物として用いるものであることを考慮すると、抽出溶媒としては安全性の面から水とエタノールとの組み合わせを用いるのが望ましい。抽出条件としては、高温、室温、低温のいずれかの温度で抽出することができるが、５０〜８０℃で１〜５時間程度が好ましい。 In order to obtain the plant extract in the present invention, it can be prepared by a conventionally known extraction method and is not particularly limited, but generally, the plant is first pulverized by an appropriate means. Next, water, methanol, ethanol, n-propanol and lower alcohols such as n-butanol, organic solvents such as ether, chloroform, ethyl acetate, acetone, glycerin, propylene glycol or a mixed solvent of two or more are added. Thus, extraction is performed by a conventional extraction method. However, considering that the product of the present invention is used as a pharmaceutical product, an oral composition, or a food or drink, it is desirable to use a combination of water and ethanol as an extraction solvent from the viewpoint of safety. As extraction conditions, extraction can be performed at any one of high temperature, room temperature, and low temperature, but preferably at 50 to 80 ° C. for about 1 to 5 hours.
このようにして得られた抽出物は、濾過し、減圧下において濃縮または凍結乾燥したものを使用することもできる。また、これらの抽出物を有機溶剤、カラムクロマトグラフィー等により分画精製したものも使用することも可能である。 The extract thus obtained can be filtered and concentrated or lyophilized under reduced pressure. In addition, it is also possible to use a product obtained by fractionating and purifying these extracts with an organic solvent, column chromatography or the like.
本発明のメチオニナーゼ阻害剤は、上記方法によって作製した植物抽出物の１種または２種以上を有効成分として使用することにより作製することができる。必要により適当な液体単体に溶解するか或いは分散させ、または適当な粉末単体と混合するか或いはこれに吸着させ、場合によっては、さらにこれに乳化剤、安定剤、分散剤等を添加して、錠剤、散剤、乳剤、水和剤等の製剤として使用してもよい。この場合の添加量としては、剤に対して乾燥抽出物を０．００１〜５０重量％、好ましくは０．０１〜２５重量％の割合になるように添加するのが好適である。 The methioninase inhibitor of the present invention can be prepared by using one or more plant extracts prepared by the above method as an active ingredient. If necessary, dissolve or disperse in an appropriate liquid alone, or mix with or adsorb to an appropriate powder alone, and optionally add an emulsifier, stabilizer, dispersant, etc. , Powders, emulsions, wettable powders and the like. As an addition amount in this case, it is suitable to add the dry extract so as to have a ratio of 0.001 to 50% by weight, preferably 0.01 to 25% by weight with respect to the agent.
また本発明のメチオニナーゼ阻害剤は、香り、呈味性に優れ、安全性が高いことから、例えば、練り歯磨、含嗽剤、消臭スプレー等の口腔用組成物、チューインガム、キャンディ、錠菓、グミゼリー、チョコレート、ビスケット等の菓子、アイスクリーム、シャーベット、氷菓等の冷菓、飲料、スープ並びにジャム等の飲食品に配合し、日常的に利用することが可能である。 Further, the methioninase inhibitor of the present invention is excellent in fragrance, taste, and safety, and therefore, for example, oral compositions such as toothpaste, gargle, deodorant spray, chewing gum, candy, tablet confectionery, gummy jelly It can be blended into confectionery such as chocolate and biscuits, frozen confectionery such as ice cream, sherbet and ice confectionery, beverages and soups, and food and drink such as jam and used on a daily basis.
添加量としては、飲食品または組成物に対して乾燥抽出物を約０．００１重量％以上、好ましくは約０．０１重量％以上添加する。さらに飲食品においては、特に嗜好性の面を考慮すると約０．００１〜５重量％、好ましくは約０．０１〜１重量％の割合になるように添加するのが好適である。 As an addition amount, about 0.001% by weight or more, preferably about 0.01% by weight or more of the dry extract is added to the food or drink or the composition. Furthermore, in the case of food / beverage products, it is preferable to add so that the ratio is about 0.001 to 5% by weight, preferably about 0.01 to 1% by weight, particularly in view of palatability.
本発明で使用する植物は、シソ、ガラナ、キンカンをはじめとして、いずれも生薬、食品素材、ハーブティ並びに天然添加物として古くより用いられているものであり、これらの抽出物並びにこれを配合した飲食品及び組成物の安全性については全く問題ない。 Plants used in the present invention, including perilla, guarana, and kumquat, have been used for a long time as herbal medicines, food materials, herbal teas, and natural additives. There is no problem with the safety of the product and the composition.
以下、実施例及び試験例を挙げて本発明品を更に詳細に説明するが、それらによって本発明品の範囲を制限するものではない。 EXAMPLES Hereinafter, although an Example and a test example are given and this invention product is demonstrated still in detail, the range of this invention product is not restrict | limited by them.
オレガノ乾燥葉粉末１０ｇに水１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を２．００ｇ得た。 Add 100 ml of water to 10 g of dried oregano leaf powder, attach a reflux condenser, and extract with reflux for 1 hour. The obtained extract was separated by filtration, the solvent was removed, and lyophilized to obtain 2.00 g of the extract.
同様にして、カラホオ樹皮、ホオノキ樹皮、アカメガシワ樹皮、クワ葉、アイスランドゴケ全草、グァバ葉、ホアハウンド地上部、ガラナ実、オオバコ種子、アルカネット根、アケビ実、クコ葉、カゴソウ花、フェンネル実、ハス葉、キンカン実、緑茶葉、シソ葉、カキ葉（対照）、ビワ葉（対照）及びスギナ地上部（対照）について、水を用いて抽出し、抽出液を濃縮または凍結乾燥することにより抽出物を調製した。抽出物の収率を表１に示した。 In the same way, Calahoo bark, Honoki bark, Akamegashi bark, Mulberry leaf, Icelandic moss whole plant, Guava leaf, Hoahound aboveground, Garana seed, Psyllium seed, Arkanet root, Akebet fruit, wolfberry leaf, Kagosou flower, Fennel fruit By extracting water, lotus leaf, kumquat fruit, green tea leaf, perilla leaf, persimmon leaf (control), loquat leaf (control), and sugiina aerial part (control), and concentrating or lyophilizing the extract An extract was prepared. The yield of the extract is shown in Table 1.
アルカネット乾燥根粉末１０ｇに５０％エタノール１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を０．５９ｇ得た。 Add 100 ml of 50% ethanol to 10 g of dried alcanet powder, attach a reflux condenser, and extract with reflux for 1 hour. The obtained extract was filtered off, the solvent was removed, and the residue was freeze-dried to obtain 0.59 g of the extract.
同様にして、カラホオ樹皮、ホオノキ樹皮、アカメガシワ樹皮、クワ葉、アイスランドゴケ全草、グァバ葉、ホアハウンド地上部、ガラナ実、オオバコ種子、アケビ実、クコ葉、カゴソウ花、オレガノ葉、フェンネル実、ハス葉、キンカン実、緑茶葉、シソ葉、カキ葉（対照）、ビワ葉（対照）及びスギナ地上部（対照）について、５０％エタノールを用いて抽出し、抽出液を濃縮または凍結乾燥することにより抽出物を調製した。抽出物の収率を表１に示した。 In the same manner, Calahoo bark, Honoki bark, Akamegashi bark, mulberry leaves, Icelandic moss whole grass, guava leaves, hoahound aerial parts, guarana seeds, psyllium seeds, akebi berries, wolfberry leaves, kagosou flower, oregano leaves, fennel fruit, Extract lotus leaf, kumquat fruit, green tea leaf, perilla leaf, oyster leaf (control), loquat leaf (control) and above-ground part of horsetail (control) using 50% ethanol, and concentrate or freeze-dry the extract. The extract was prepared by The yield of the extract is shown in Table 1.
アイスランドゴケ粉末１０ｇに７０％エタノール１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を０．８８ｇ得た。 Add 100 ml of 70% ethanol to 10 g of Icelandic moss powder, attach a reflux condenser and extract with reflux for 1 hour. The obtained extract was separated by filtration, the solvent was removed, and freeze-dried to obtain 0.88 g of the extract.
同様にして、カラホオ樹皮、ホオノキ樹皮、アカメガシワ樹皮、グァバ葉及びホアハウンド地上部について、７０％エタノールを用いて抽出し、抽出液を濃縮または凍結乾燥することにより抽出物を調製した。抽出物の収率を表１に示した。 Similarly, Calahoo bark, Honoki bark, Akamegashi bark, guava leaf and hoahound above-ground part were extracted using 70% ethanol, and the extract was concentrated or freeze-dried to prepare an extract. The yield of the extract is shown in Table 1.
ホアハウンド地上部粉末１０ｇに１００％エタノール１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を０．６０ｇ得た。 Add 100 ml of 100% ethanol to 10 g of Hoahound aboveground powder, attach a reflux condenser and extract with reflux for 1 hour. The obtained extract was separated by filtration, the solvent was removed, and freeze-dried to obtain 0.60 g of the extract.
同様にして、カラホオ樹皮、ホオノキ樹皮、アカメガシワ樹皮、クワ葉、アイスランドゴケ全草、グァバ葉、ガラナ実、オオバコ種子、アルカネット根、アケビ実、クコ葉、カゴソウ花、オレガノ葉、フェンネル実、ハス葉、キンカン実、緑茶葉、シソ葉、カキ葉（対照）、ビワ葉（対照）及びスギナ地上部（対照）について１００％エタノールを用いて抽出し、抽出液を濃縮または凍結乾燥することにより抽出物を調製した。各抽出物の収率を表１に示した。 In the same way, Calahoo bark, Honoki bark, Akamegashi bark, mulberry leaves, Icelandic moss whole plant, guava leaves, guarana seeds, psyllium seeds, alcanette roots, akebi seeds, wolfberry leaves, kagosou flower, oregano leaves, fennel fruit, Extracting lotus leaves, kumquat, green tea leaves, perilla leaves, oyster leaves (control), loquat leaves (control) and sugi (Japanese cedar) using 100% ethanol, and concentrating or freeze-drying the extract An extract was prepared. The yield of each extract is shown in Table 1.
クワ葉粉末１０ｇに１００％酢酸エチル１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を４．０９ｇ得た。 Add 100 ml of 100% ethyl acetate to 10 g of mulberry leaf powder, attach a reflux condenser and extract with reflux for 1 hour. The obtained extract was separated by filtration, the solvent was removed, and freeze-dried to obtain 4.09 g of the extract.
同様にして、カラホオ樹皮、ホオノキ樹皮、オオバコ種子、アルカネット根及びフェンネル実について１００％酢酸エチルを用いて抽出し、抽出液を濃縮または凍結乾燥することにより抽出物を調製した。各抽出物の収率を表１に示した。 Similarly, Calahoo bark, honoki bark, psyllium seed, alkanette root and fennel fruit were extracted using 100% ethyl acetate, and the extract was prepared by concentrating or freeze-drying the extract. The yield of each extract is shown in Table 1.
ホオノキ樹皮粉末１０ｇに１００％アセトン１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を２．５３ｇ得た。 Add 100 ml of 100% acetone to 10 g of honoki bark powder, attach a reflux condenser, and extract with reflux for 1 hour. The obtained extract was filtered off, the solvent was removed, and the residue was freeze-dried to obtain 2.53 g of the extract.
同様にして、カラホオ樹皮、アイスランドゴケ全草及びオレガノ葉について１００％アセトンを用いて抽出し、抽出液を濃縮または凍結乾燥することにより抽出物を調製した。各抽出物の収率を表１に示した。 Similarly, Calahoo bark, Icelandic moss whole grass and oregano leaves were extracted with 100% acetone, and the extract was concentrated or freeze-dried to prepare an extract. The yield of each extract is shown in Table 1.
アイスランドゴケ粉末１０ｇに１００％ｎ−ブタノール１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を０．７７ｇ得た。 100 g of 100% n-butanol is added to 10 g of Icelandic moss powder, a reflux condenser is attached, and extraction is performed while refluxing for 1 hour. The obtained extract was filtered off, the solvent was removed, and the residue was freeze-dried to obtain 0.77 g of the extract.
同様にして、オオバコ種子及びクコ葉について１００％ｎ−ブタノールを用いて抽出し、抽出液を濃縮または凍結乾燥することにより抽出物を調製した。各抽出物の収率を表１に示した。 Similarly, psyllium seeds and wolfberry leaves were extracted with 100% n-butanol, and the extract was prepared by concentrating or lyophilizing the extract. The yield of each extract is shown in Table 1.
ホアハウンド地上部粉末１０ｇに１００％メタノール１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を０．８０ｇ得た。抽出物の収率を表１に示した。 Add 100 ml of 100% methanol to 10 g of Hoahound aboveground powder, attach a reflux condenser, and extract with reflux for 1 hour. The obtained extract was filtered off, the solvent was removed, and freeze-dried to obtain 0.80 g of the extract. The yield of the extract is shown in Table 1.
オオバコ種子粉末１０ｇに１００％エーテル１００ｍｌを加え、還流冷却器をつけて、１時間還流しながら抽出する。得られた抽出液を濾別し、溶媒を除去した後、凍結乾燥することにより抽出物を０．８９ｇ得た。抽出物の収率を表１に示した。 Add 100 ml of 100% ether to 10 g of psyllium seed powder, attach a reflux condenser, and extract with reflux for 1 hour. The obtained extract was separated by filtration, the solvent was removed, and freeze-dried to obtain 0.89 g of the extract. The yield of the extract is shown in Table 1.
メチオニンはメチオニナーゼの反応によりメチルメルカプタン、アンモニア及び２−ケト酪酸が生成する。この反応生成物の中で比較的、安定な物質である２−ケト酪酸の生成量を酵素反応の指標とした。具体的な方法としては、嫌気条件で２日間培養したフゾバクテリウム・ヌクレイタムＡＴＣＣ２５５８６を超音波処理により破壊することで得た酵素、メチオニン、ピリドキサールリン酸及び素材（終濃度：２００μｇ／ｍｌ）を、リン酸緩衝液（５０ｍＭ、ｐＨ７．６）中で混合した。３７℃で１時間反応させた後、０．５ｍｌの反応液を同量の過塩素酸水溶液（６％）と混合し、タンパク質を変性させた後、３０００×ｇ、１０分間遠心して沈殿物を取り除き試料液とした。反応の副生成物である２−ケト酪酸を定量するために、試料液０．５ｍｌに０．０５％３−メチル−２−ベンゾチアゾリノンヒドラゾン（ＭＢＴＨ）溶液０．５ｍｌと１Ｍ酢酸ナトリウム緩衝液（ｐＨ５．０）１ｍｌを混合し、５０℃で３０分間反応させた。反応後、反応液が室温にまで低下したことを確認し、吸光度（３３５ｎｍ）を測定した。 Methionine produces methyl mercaptan, ammonia and 2-ketobutyric acid by the reaction of methioninase. Among these reaction products, the amount of 2-ketobutyric acid, which is a relatively stable substance, was used as an index for the enzyme reaction. As a specific method, an enzyme, methionine, pyridoxal phosphate, and a material (final concentration: 200 μg / ml) obtained by disrupting Fusobacterium nucleatum ATCC25586 cultured for 2 days under anaerobic conditions by sonication were used. Mixed in buffer (50 mM, pH 7.6). After reacting at 37 ° C. for 1 hour, 0.5 ml of the reaction solution was mixed with the same volume of perchloric acid aqueous solution (6%) to denature the protein, and then centrifuged at 3000 × g for 10 minutes. The sample solution was removed. To quantify 2-ketobutyric acid, a byproduct of the reaction, 0.5 ml of 0.05% 3-methyl-2-benzothiazolinone hydrazone (MBTH) solution and 1 M sodium acetate buffer were added to 0.5 ml of the sample solution. 1 ml of liquid (pH 5.0) was mixed and reacted at 50 ° C. for 30 minutes. After the reaction, it was confirmed that the reaction solution had dropped to room temperature, and the absorbance (335 nm) was measured.
ただし、評価素材の色が２−ケト酪酸の定量反応に多少の影響を与えることから、サンプルブランク（上記反応でメチオニンだけを除いた反応液）を差し引くことで吸光度の補正を行なった。 However, since the color of the evaluation material has some influence on the quantitative reaction of 2-ketobutyric acid, the absorbance was corrected by subtracting the sample blank (reaction liquid from which only methionine was removed in the above reaction).
２−ケト酪酸量は予め作成した検量線により求め、下記計算式により阻害率を算出した。 The amount of 2-ketobutyric acid was determined from a calibration curve prepared in advance, and the inhibition rate was calculated using the following formula.
阻害率（％）＝（（Ｃ−Ｓ）／Ｃ）×１００
式中、Ｃはコントロールの２−ケト酪酸量、Ｓは試料添加時の２−ケト酪酸量である。
Inhibition rate (%) = ((C−S) / C) × 100
In the formula, C is the amount of control 2-ketobutyric acid, and S is the amount of 2-ketobutyric acid at the time of sample addition.
メチオニナーゼに対する阻害効果の測定結果を表２に示した。口腔内細菌（ポルフィロモナス・ジンジバリス）由来メチオニナーゼ阻害効果が知られているカキ葉抽出物、ビワ葉抽出物及びスギナ地上部抽出物（特開２００２−３３５３号公報）を対照として評価したところ、阻害率はカキ葉抽出物で４０．４％、ビワ葉抽出物で１４．８％、スギナ地上部抽出物で２１．０％であった。本発明品であるカラホオ樹皮及び実、ホオノキ樹皮、アカメガシワ樹皮、クワ葉、アイスランドゴケ全草、グァバ葉、ホアハウンド地上部、ガラナ実、オオバコ種子、アルカネット根、アケビ実、クコ葉、カゴソウ花、オレガノ葉、フェンネル実、ハス葉、キンカン実、緑茶抽出物及びシソ葉の抽出物はいずれもメチオニナーゼに対して高い阻害活性を示した。特にオオバコ種子、ホアハウンド全草、カラホオ樹皮、ホオノキ樹皮及びクワ葉抽出物が９０％を超える非常に強い活性を有することがわかった。 The measurement results of the inhibitory effect on methioninase are shown in Table 2. When oyster leaf extract, loquat leaf extract and Japanese horsetail extract from which oral bacterium (Porphyromonas gingivalis) derived methioninase inhibitory effect is known were evaluated as a control, The inhibition rate was 40.4% for the oyster leaf extract, 14.8% for the loquat leaf extract, and 21.0% for the cedar aerial extract. Calahoo bark and fruit, honoki bark, red mulberry bark, mulberry leaf, Icelandic moss whole grass, guava leaf, guahound above ground, guarana fruit, psyllium seeds, alcanet root, akebi fruit, wolfberry leaf, kagosou flower The oregano leaf, fennel fruit, lotus leaf, kumquat fruit, green tea extract and perilla leaf extract all showed high inhibitory activity against methioninase. In particular, it was found that psyllium seed, hoahound whole grass, calahoo bark, honoki bark and mulberry leaf extract have very strong activity exceeding 90%.
以上の試験結果により、本発明品であるカラホオ、ホオノキ、アカメガシワ、クワ、アイスランドゴケ、グァバ、ホアハウンド、ガラナ、オオバコ、アルカネット、アケビ、クコ、カゴソウ、オレガノ、フェンネル、ハス、キンカン、緑茶及びシソより得られた抽出物は、メチオニナーゼ阻害活性を持つことが今回初めて明らかになった。 Based on the above test results, the product of the present invention, Calahoo, Honoki, Akamegashiwa, Mulberry, Icelandic moss, Guava, Hoahound, Guarana, Psyllium, Alcanet, Akebi, Kuko, Kagosou, Oregano, Fennel, Lotus, Kumquat, Green tea and It has been revealed for the first time that an extract obtained from perilla has methioninase inhibitory activity.
実施例１、２、３、４、５、６、７、８及び９で示した方法により調製した本発明品を用いて、以下の処方により、練り歯磨、含嗽剤、消臭スプレー、口臭用スプレー、錠剤、粉末剤等の組成物、チューインガム、キャンディ、錠菓、グミゼリー、チョコレート、ビスケット等の菓子、アイスクリーム、シャーベット、氷菓等の冷菓、飲料、スープ及びジャム等の飲食品を製造した。 Using the products of the present invention prepared by the methods shown in Examples 1, 2, 3, 4, 5, 6, 7, 8 and 9, toothpaste, gargle, deodorant spray, bad breath Compositions such as sprays, tablets and powders, chewing gums, candy, tablet confectionery, gummy jelly, chocolate, biscuits and other confectionery, ice cream, sorbets, frozen confectionery such as ice confectionery, beverages, soups and jams and other food and drink were produced.
練り歯磨の処方
炭酸カルシウム ５０．０重量％
グリセリン ２０．０
カルボオキシメチルセルロース ２．０
ラウリル硫酸ナトリウム ２．０
香料 １．０
サッカリン ０．１
ホオノキ樹皮１００％エタノール抽出物（実施例４） １．０
クロルヘキシジン ０．０１
水 残
１００．０
Toothpaste prescription calcium carbonate 50.0% by weight
Glycerin 20.0
Carboxymethylcellulose 2.0
Sodium lauryl sulfate 2.0
Fragrance 1.0
Saccharin 0.1
Honoki bark 100% ethanol extract (Example 4) 1.0
Chlorhexidine 0.01
Water remaining
100.0
含嗽剤の処方
エタノール ２．０ 重量％
香料 １．０
サッカリン ０．０５
塩酸クロルヘキシジン ０．０１
緑茶葉５０％エタノール抽出物（実施例２） ０．５
水 残
１００．０
Prescription of gargle ethanol 2.0% by weight
Fragrance 1.0
Saccharin 0.05
Chlorhexidine hydrochloride 0.01
Green tea leaf 50% ethanol extract (Example 2) 0.5
Water remaining
100.0
口臭用スプレーの処方
エタノール １０．０重量％
グリセリン ５．０
アカメガシワ樹皮５０％エタノール抽出物（実施例２） １．０
香料 ０．０５
着色料 ０．００１
水 残
１００．０
Prescription ethanol for bad breath spray 10.0% by weight
Glycerin 5.0
Acamegawashi bark 50% ethanol extract (Example 2) 1.0
Fragrance 0.05
Coloring 0.001
Water remaining
100.0
トローチ剤の処方
ブドウ糖 ７２．３重量％
乳糖 １９．０
アラビアゴム ６．０
香料 １．０
モノフルオロリン酸ナトリウム ０．７
カラホオ樹皮７０％エタノール抽出物（実施例３） １．０
１００．０
Troche prescription glucose 72.3% by weight
Lactose 19.0
Gum arabic 6.0
Fragrance 1.0
Sodium monofluorophosphate 0.7
Calahoo bark 70% ethanol extract (Example 3) 1.0
100.0
チューインガムの処方
ガムベース ２０．０重量％
砂糖 ５５．０
グルコース １５．０
水飴 ９．０
香料 ０．５
オオバコ種子１００％酢酸エチル抽出物（実施例５） ０．５
１００．０
Chewing gum formula gum base 20.0% by weight
Sugar 55.0
Glucose 15.0
Minamata 9.0
Fragrance 0.5
Psyllium seed 100% ethyl acetate extract (Example 5) 0.5
100.0
キャンディの処方
砂糖 ５０．０重量％
水飴 ３４．０
香料 ０．５
オレガノ葉１００％アセトン抽出物（実施例６） ０．５
水 残
１００．０
Candy prescription sugar 50.0% by weight
Minamata 34.0
Fragrance 0.5
Oregano leaf 100% acetone extract (Example 6) 0.5
Water remaining
100.0
錠菓の処方
砂糖 ７６．４重量％
グルコース １９．０
ショ糖脂肪酸エステル ０．２
香料 ０．２
オオバコ種子１００％エーテル抽出物（実施例９） ０．１
水 残
１００．０
Prescription sugar for tablet candy 76.4% by weight
Glucose 19.0
Sucrose fatty acid ester 0.2
Fragrance 0.2
Psyllium seed 100% ether extract (Example 9) 0.1
Water remaining
100.0
グミゼリーの処方
ゼラチン ６０．０重量％
水飴 ２３．０
砂糖 ８．５
植物油脂 ４．５
マンニトール ２．９５
レモン果汁 １．０
クコ葉１００％ｎ−ブタノール抽出物（実施例７） ０．０５
１００．０
Gummy jelly prescription gelatin 60.0% by weight
Minamata 23.0
8.5 sugar
Vegetable oil 4.5
Mannitol 2.95
Lemon juice 1.0
Wolfberry leaf 100% n-butanol extract (Example 7) 0.05
100.0
チョコレートの処方
粉糖 ４１．８重量％
カカオビター ２０．０
全脂粉乳 ２０．０
カカオバター １７．０
マンニトール ２．９５
シソ葉水抽出物（実施例１） １．０
香料 ０．２
１００．０
Chocolate formula powdered sugar 41.8% by weight
Cocoa bitter 20.0
Whole milk powder 20.0
Cocoa butter 17.0
Mannitol 2.95
Perilla leaf water extract (Example 1) 1.0
Fragrance 0.2
100.0
ビスケットの処方
薄力１級 ２５．５９重量％
中力１級 ２２．２２
精白糖 ４．８
食塩 ０．７３
ブドウ糖 ０．７８
パームショートニング １１．７８
炭酸水素ナトリウム ０．１７
重亜硫酸ナトリウム ０．１６
米粉 １．４５
全脂粉乳 １．１６
代用粉乳 ０．２９
ホアハウンド地上部１００％メタノール抽出物（実施例８） ０．５
水 残
１００．０
Biscuit prescription thinness grade 1 25.59% by weight
Intermediate 1st class 22.22
Refined sugar 4.8
Salt 0.73
Glucose 0.78
Palm shortening 11.78
Sodium bicarbonate 0.17
Sodium bisulfite 0.16
Rice flour 1.45
Whole milk powder 1.16
Substitute milk powder 0.29
Hoahound above-ground 100% methanol extract (Example 8) 0.5
Water remaining
100.0
アイスクリームの処方
脱脂粉乳 ５０．０重量％
生クリーム ２５．０
砂糖 １０．０
卵黄 １０．０
フェンネル実１００％エタノール抽出物（実施例４） １．０
香料 ０．１
水 残
１００．０
Ice cream formula nonfat dry milk 50.0% by weight
Fresh cream 25.0
Sugar 10.0
Egg yolk 10.0
Fennel real 100% ethanol extract (Example 4) 1.0
Fragrance 0.1
Water remaining
100.0
シャーベットの処方
オレンジ果汁 ２５．０重量％
砂糖 ２５．０
卵白 １０．０
アルカネット根水抽出物（実施例１） ２．０
水 残
１００．０
Sherbet prescription orange juice 25.0% by weight
Sugar 25.0
Egg white 10.0
Alcanet root water extract (Example 1) 2.0
Water remaining
100.0
飲料の処方
オレンジ果汁 ３０．０重量％
異性化糖 １５．２４
クエン酸 ０．１
ビタミンＣ ０．０４
香料 ０．１
グァバ葉７０％エタノール抽出物（実施例３） １．０
水 残
１００．０
Prescription orange juice 30.0% by weight
Isomerized sugar 15.24
Citric acid 0.1
Vitamin C 0.04
Fragrance 0.1
Guava leaf 70% ethanol extract (Example 3) 1.0
Water remaining
100.0
スープの処方
牛乳 ６０．００重量％
たまねぎ ２０．００
にんじん １０．００
野菜ブイヨン １．００
バター ０．１０
コショウ ０．０５
塩 ０．０５
オレガノ葉１００％エタノール抽出物（実施例４） １．０
水 残
１００．０
Soup prescription milk 60.00 wt%
Onion 20.00
Carrot 10.00
Vegetable bouillon 1.00
Butter 0.10
Pepper 0.05
0.05 salt
Oregano leaf 100% ethanol extract (Example 4) 1.0
Water remaining
100.0
ジャムの処方
果肉 ４．０重量％
砂糖 ６５．０
清澄果汁 ２５．０
クエン酸 ０．５
アケビ実１００％エタノール抽出物（実施例４） ２．０
水 残
１００．０
Prescription pulp of jam 4.0% by weight
Sugar 65.0
Kiyosumi juice 25.0
Citric acid 0.5
Akebi 100% ethanol extract (Example 4) 2.0
Water remaining
100.0
練り歯磨の処方
炭酸カルシウム ５０．０重量％
グリセリン ２０．０
カルボオキシメチルセルロース ２．０
ラウリル硫酸ナトリウム ２．０
香料 １．０
サッカリン ０．１
カラホオ樹皮１００％エタノール抽出物（実施例４） １．０
ホオノキ樹皮７０％エタノール抽出物（実施例３） １．０
クロルヘキシジン ０．０１
水 残
１００．０
Toothpaste prescription calcium carbonate 50.0% by weight
Glycerin 20.0
Carboxymethylcellulose 2.0
Sodium lauryl sulfate 2.0
Fragrance 1.0
Saccharin 0.1
Calahoo bark 100% ethanol extract (Example 4) 1.0
Honoki bark 70% ethanol extract (Example 3) 1.0
Chlorhexidine 0.01
Water remaining
100.0
口臭用スプレーの処方
エタノール １０．０重量％
グリセリン ５．０
クワ葉１００％エタノール抽出物（実施例４） １．０
アイスランドゴケ７０％エタノール抽出物（実施例３） １．０
香料 ０．０５
着色料 ０．００１
水 残
１００．０
Prescription ethanol for bad breath spray 10.0% by weight
Glycerin 5.0
Mulberry leaf 100% ethanol extract (Example 4) 1.0
Iceland Poke 70% ethanol extract (Example 3) 1.0
Fragrance 0.05
Coloring 0.001
Water remaining
100.0
チューインガムの処方
ガムベース ２０．０重量％
砂糖 ５５．０
グルコース １５．０
水飴 ９．０
香料 ０．５
ガラナ実１００％エタノール抽出物（実施例４） ０．５
ハス葉１００％エタノール抽出物（実施例４） ０．５
１００．０
Chewing gum formula gum base 20.0% by weight
Sugar 55.0
Glucose 15.0
Minamata 9.0
Fragrance 0.5
Guarana 100% ethanol extract (Example 4) 0.5
Lotus leaf 100% ethanol extract (Example 4) 0.5
100.0
キャンディの処方
砂糖 ５０．０重量％
水飴 ３４．０
香料 ０．５
クコ葉１００％ｎ−ブタノール抽出物（実施例７） ０．５
カゴソウ花１００％エタノール抽出物（実施例４） ０．５
水 残
１００．０
Candy prescription sugar 50.0% by weight
Minamata 34.0
Fragrance 0.5
Wolfberry leaf 100% n-butanol extract (Example 7) 0.5
Kagosou flower 100% ethanol extract (Example 4) 0.5
Water remaining
100.0
錠菓の処方
砂糖 ７６．４重量％
グルコース １９．０
ショ糖脂肪酸エステル ０．２
香料 ０．２
キンカン実１００％エタノール抽出物（実施例４） ０．１
シソ葉１００％エタノール抽出物（実施例４） ０．１
水 残
１００．０
Prescription sugar for tablet candy 76.4% by weight
Glucose 19.0
Sucrose fatty acid ester 0.2
Fragrance 0.2
Kumquat fruit 100% ethanol extract (Example 4) 0.1
Perilla leaf 100% ethanol extract (Example 4) 0.1
Water remaining
100.0
錠剤の処方
カラホオ樹皮５０％エタノール抽出物（実施例２） １０．０重量％
ラクトース ７０．０
結晶性セルロース １５．０
ステアリン酸マグネシウム ５．０
１００．０
Tablet formulation Calahoo bark 50% ethanol extract (Example 2) 10.0 wt%
Lactose 70.0
Crystalline cellulose 15.0
Magnesium stearate 5.0
100.0
前記成分を細かく粉砕混合した後、直打法（ｄｉｒｅｃｔｔａｂｌｅｔｉｎｇ ｍｅｔｈｏｄ）によって錠剤を製造した。各錠剤の総量は１００ｍｇであり、そのうちの有効成分は１０ｍｇである。 After the above components were finely pulverized and mixed, tablets were produced by a directing method. The total amount of each tablet is 100 mg, of which the active ingredient is 10 mg.
粉末剤の処方
アカメガシワ樹皮水抽出物（実施例１） ５．０重量％
トウモロコシ澱粉 ５５．０
カルボキシセルロース ４０．０
１００．０
Powder formulation Akamegasiwa bark water extract (Example 1) 5.0 wt%
Corn starch 55.0
Carboxycellulose 40.0
100.0
前記成分を細かく粉砕混合して粉末を製造した。硬質カプセルに粉末１００ｍｇを入れてカプセル剤を製造した。 The above ingredients were finely pulverized and mixed to produce a powder. A capsule was prepared by putting 100 mg of powder into a hard capsule.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014039776A JP2014111652A (en) | 2014-02-28 | 2014-02-28 | Methioninase inhibitor and composition for oral cavity and food and drink containing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014039776A JP2014111652A (en) | 2014-02-28 | 2014-02-28 | Methioninase inhibitor and composition for oral cavity and food and drink containing the same |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011211682A Division JP5539941B2 (en) | 2011-09-27 | 2011-09-27 | Methioninase inhibitor and oral composition containing the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2014111652A true JP2014111652A (en) | 2014-06-19 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014039776A Pending JP2014111652A (en) | 2014-02-28 | 2014-02-28 | Methioninase inhibitor and composition for oral cavity and food and drink containing the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2014111652A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016084311A (en) * | 2014-10-28 | 2016-05-19 | 国立大学法人広島大学 | Mouth odor inhibitor and oral composition |
| WO2017007140A1 (en) * | 2015-07-08 | 2017-01-12 | 대한민국(환경부 국립생물자원관장) | Oral composition for preventing and treating periodontitis, comprising mulberry root bark extract and winged spindle extract as active ingredients |
| JP2017189160A (en) * | 2016-04-12 | 2017-10-19 | キム ソン ペＫｉｍ Ｓｏｎｇ Ｂａｅ | Manufacturing method of akebia quinata extract and functional food using the extract |
| US10195904B2 (en) | 2014-05-29 | 2019-02-05 | Bridgestone Corporation | Pneumatic tire |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5933231A (en) * | 1982-08-19 | 1984-02-23 | Seikou Kin | Apparatus for extracting chlorophyll |
| JPS63307881A (en) * | 1987-06-08 | 1988-12-15 | Osaka Seiken:Kk | Method for inhibiting mutagen activity |
| JPH05271085A (en) * | 1992-03-30 | 1993-10-19 | Sunstar Inc | Agent for preventing periodontosis |
| JPH0640868A (en) * | 1992-07-16 | 1994-02-15 | Lotte Co Ltd | Composition for oral cavity application |
| JPH11246424A (en) * | 1998-02-25 | 1999-09-14 | Maruzen Seiyaku Kk | Tyrosinase inhibitor and its production |
-
2014
- 2014-02-28 JP JP2014039776A patent/JP2014111652A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5933231A (en) * | 1982-08-19 | 1984-02-23 | Seikou Kin | Apparatus for extracting chlorophyll |
| JPS63307881A (en) * | 1987-06-08 | 1988-12-15 | Osaka Seiken:Kk | Method for inhibiting mutagen activity |
| JPH05271085A (en) * | 1992-03-30 | 1993-10-19 | Sunstar Inc | Agent for preventing periodontosis |
| JPH0640868A (en) * | 1992-07-16 | 1994-02-15 | Lotte Co Ltd | Composition for oral cavity application |
| JPH11246424A (en) * | 1998-02-25 | 1999-09-14 | Maruzen Seiyaku Kk | Tyrosinase inhibitor and its production |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10195904B2 (en) | 2014-05-29 | 2019-02-05 | Bridgestone Corporation | Pneumatic tire |
| JP2016084311A (en) * | 2014-10-28 | 2016-05-19 | 国立大学法人広島大学 | Mouth odor inhibitor and oral composition |
| WO2017007140A1 (en) * | 2015-07-08 | 2017-01-12 | 대한민국(환경부 국립생물자원관장) | Oral composition for preventing and treating periodontitis, comprising mulberry root bark extract and winged spindle extract as active ingredients |
| JP2017189160A (en) * | 2016-04-12 | 2017-10-19 | キム ソン ペＫｉｍ Ｓｏｎｇ Ｂａｅ | Manufacturing method of akebia quinata extract and functional food using the extract |
| US10463706B2 (en) | 2016-04-12 | 2019-11-05 | Song Bae Kim | Method of preparing akebia quinata extract and functional food using the same |
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