JP2013108774A - Pressure inspection device - Google Patents

Pressure inspection device Download PDF

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JP2013108774A
JP2013108774A JP2011252077A JP2011252077A JP2013108774A JP 2013108774 A JP2013108774 A JP 2013108774A JP 2011252077 A JP2011252077 A JP 2011252077A JP 2011252077 A JP2011252077 A JP 2011252077A JP 2013108774 A JP2013108774 A JP 2013108774A
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filter
pressure
main surfaces
flow rate
gas layer
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Tetsuya Nishizaki
哲也 西▲崎▼
Eiji Kawada
栄二 川田
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Toppan Inc
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Toppan Printing Co Ltd
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Abstract

PROBLEM TO BE SOLVED: To provide a pressure inspection device and the like capable of detecting a failure or the like (whether or not there is a tear, a chip, or a gap, etc.) of a filter (typically, filter for purifying a genome) made of a porous material capable of adsorbing a genome in a dissolved cell.SOLUTION: A pressure inspection device comprises: pressurizing means (600, 1) for pressurizing, with a gas layer (typically, air layer), one of the main surfaces of a filter (23) that is made of a porous material capable of adsorbing a genome in a dissolved cell and that contains a predetermined liquid (typically, pure water); and pressure detection means (500) for detecting pressure of the air layer on one of the main surfaces pressurized by the pressurizing means.

Description

本発明は、フィルタの不良（破れ、欠け、隙間の有無等）等を圧力値にて検知することのできる圧力検査装置に関する。   The present invention relates to a pressure inspection apparatus capable of detecting a filter failure (breakage, chipping, presence of gaps, etc.) by a pressure value.

遺伝子解析研究において、人などの血液よりゲノムを自動的に精製（抽出）するシステムに、材質がガラス等で構成されている円形の上記フィルタを用いており、当該フィルタにゲノムを吸着させ、空気層による圧力を掛けることで或る程度の量のゲノムが精製される仕組みとなっている。   In gene analysis research, the above-mentioned circular filter made of glass or the like is used in a system that automatically purifies (extracts) genomes from human blood. A certain amount of genome is purified by applying pressure by the layer.

また、前記フィルタは樹脂成型等にて作製されたフィルタホルダに組み込まれた状態で使用され、前記フィルタホルダには前記フィルタの外径と同等の内径の設置箇所が設けられている。   The filter is used in a state of being incorporated in a filter holder manufactured by resin molding or the like, and the filter holder is provided with an installation location having an inner diameter equivalent to the outer diameter of the filter.

前記フィルタを、前記フィルタホルダに組み付けた状態で使用することにより、前記フィルタホルダが器となり、人などの血液以外にゲノム精製に必要な溶液を分注ならびに加圧することが可能となる。   By using the filter in a state assembled to the filter holder, the filter holder becomes a vessel, and it is possible to dispense and pressurize a solution necessary for genome purification in addition to blood such as humans.

しかし、前記フィルタに破れや欠け等があり、又、前記フィルタを前記フィルタホルダに組み付けた際に位置ずれが生じた場合、前記フィルタホルダと前記フィルタの端部に隙間ができ、この状態で加圧を行った場合、前記フィルタ全面に均一な圧力が掛からず、前記フィルタにゲノムが付着し辛くなり、又、前記フィルタに付着しているゲノムや溶液等が残留してしまい、その結果、得られるゲノムの精製量（抽出量）が減少してしまう。また、反応に関する不具合の発生率が高くなる。   However, if the filter is torn or chipped, or if the filter is displaced when the filter is assembled to the filter holder, a gap is created between the filter holder and the end of the filter. When pressure is applied, uniform pressure is not applied to the entire surface of the filter, and it is difficult for the genome to adhere to the filter, and the genome, solution, etc. attached to the filter remain. The amount of purified (extracted) genome is reduced. In addition, the occurrence rate of defects related to the reaction is increased.

人などの血液からゲノムを精製する工程をより詳細に説明すると、（１）血液の溶解 → （２）溶解した血液を前記フィルタ上部へ移動させ、ゲノムを吸着させる → （３）前記フィルタの洗浄 → （４）前記フィルタの乾燥 → （５）前記フィルタからゲノムの回収、という流れとなる。   The process of purifying the genome from human blood will be described in more detail. (1) Lysis of blood → (2) Move the dissolved blood to the top of the filter and adsorb the genome → (3) Wash the filter → (4) Drying of the filter → (5) Recovery of genome from the filter.

上記工程の（２）〜（５）は全て、前記フィルタに空気層による圧力を掛ける必要があり、例えば（２）溶解した血液を前記フィルタ上部へ移動させ、ゲノムを吸着させる工程において、前記フィルタに破れ、欠け等、また前記フィルタホルダと前記フィルタの端部に隙間が生じている場合、溶解した血液の一部は前記フィルタの全面を通過することなく、前記フィルタの破れ、隙間等から流れ出てしまう。   In the above steps (2) to (5), it is necessary to apply pressure by an air layer to the filter. For example, in the step of (2) moving the dissolved blood to the upper part of the filter and adsorbing the genome, the filter If there is a gap between the filter holder and the end of the filter, a part of the dissolved blood does not pass through the entire surface of the filter and flows out of the filter. End up.

また（４）前記フィルタの乾燥工程においては、前記フィルタに破れ、欠け等、また前記フィルタホルダと前記フィルタの端部に隙間が生じている場合、空気層による圧力が前記フィルタ全面に均一に掛からず、前記フィルタの破れ、隙間等から流れ出てしまい乾燥が不十分となり、その結果、（３）前記フィルタの洗浄工程で用いた洗浄液中のエタノールが残留してしまい、前記フィルタからゲノムを回収した際に、回収したゲノム（溶液）にエタノールが混入してしまい、回収したゲノムを用いた反応に関する不具合の発生率が高くなる。   (4) In the drying process of the filter, when the filter is torn or chipped, or a gap is formed between the filter holder and the end of the filter, the pressure of the air layer is applied uniformly over the entire surface of the filter. First, the filter was broken and flowed out from the gaps and the like, resulting in insufficient drying. As a result, (3) ethanol in the cleaning solution used in the filter cleaning process remained, and the genome was collected from the filter. At this time, ethanol is mixed into the collected genome (solution), and the occurrence rate of defects related to the reaction using the collected genome is increased.

ここで、管路等からの漏れを検出する従来技術として、例えば、特許文献１及び２がある。   Here, as a prior art for detecting a leak from a pipeline or the like, for example, there are Patent Documents 1 and 2.

特開２００７−２３２６６６号公報JP 2007-232666 A 特開平１１−３４４４１２号公報Japanese Patent Application Laid-Open No. 11-344412

しかしながら、上記した従来技術では、以上に説明した問題を有効に解決できなかった。   However, the above-described conventional technology cannot effectively solve the problems described above.

それ故に、本発明の目的は、溶解された細胞中のゲノムを吸着できる多孔質材料から成るフィルタ（典型的には、ゲノム精製用フィルタ）の不良等（破れ、欠け、隙間の有無等）を検知できる圧力検査装置等を提供することである。   Therefore, it is an object of the present invention to eliminate a defect (typically, a genome purification filter) made of a porous material capable of adsorbing a genome in a lysed cell (such as the presence or absence of a tear, a chip or a gap). It is to provide a pressure inspection device that can be detected.

上記の目的を達成するために、本発明は以下の構成を採用した。なお、括弧内の参照符号、説明文言等は、本発明の理解を助けるために後述する実施形態との対応関係を示したものであって、本発明の範囲を何ら限定するものではない。   In order to achieve the above object, the present invention employs the following configuration. In addition, reference numerals in parentheses, explanations, and the like indicate correspondence with embodiments described later in order to help understanding of the present invention, and do not limit the scope of the present invention.

圧力検査装置であって、溶解された細胞中のゲノムを吸着できる多孔質材料から成り、所定の液体（典型的には、純水）を含んだ状態のフィルタ（２３）の主面の一方に対して、気体層（典型的には、空気層）によって圧力をかける加圧手段（６００、１）と、前記加圧手段によって圧力がかけられた前記主面の一方における前記気体層の圧力を検出する圧力検出手段（５００）とを備える。   A pressure testing device, which is made of a porous material capable of adsorbing a lysed genome of cells and includes a predetermined liquid (typically pure water) on one of the main surfaces of the filter (23). On the other hand, the pressure means (600, 1) for applying pressure by the gas layer (typically, the air layer) and the pressure of the gas layer on one of the main surfaces to which pressure is applied by the pressure means Pressure detecting means (500) for detecting.

また、前記加圧手段は、前記フィルタが組み付けられることによって閉塞された筒部材（フィルタホルダ２１）の一方の開放端側に対して圧力をかけることによって、当該フィルタの主面の一方に対して圧力をかけてもよい。   Further, the pressurizing means applies pressure to one open end side of the cylindrical member (filter holder 21) closed by assembling the filter, so that one of the main surfaces of the filter is applied. Pressure may be applied.

また、前記加圧手段によって前記フィルタの主面の一方に対して圧力をかけるための前記気体層の流量を検出する流量検出手段（４００）と、前記気体層の流量を調整する流量調整手段（３００）とを備えてもよい。   Further, a flow rate detecting means (400) for detecting the flow rate of the gas layer for applying pressure to one of the principal surfaces of the filter by the pressurizing means, and a flow rate adjusting means (for adjusting the flow rate of the gas layer) 300).

また、前記加圧手段によって前記フィルタの主面の一方に対してかけられる圧力を設定する圧力設定手段（２００）を備えてもよい。   Moreover, you may provide the pressure setting means (200) which sets the pressure applied with respect to one of the main surfaces of the said filter by the said pressurization means.

なお、以上では、本発明を圧力検査装置として記載したが、本発明は、圧力検査方法としてとらえることもできる。   In the above, the present invention has been described as a pressure inspection device, but the present invention can also be regarded as a pressure inspection method.

本発明によれば、溶解された細胞中のゲノムを吸着できる多孔質材料から成るフィルタ（典型的には、ゲノム精製用フィルタ）の不良等（破れ、欠け、隙間の有無等）を検知できる。また、本発明によれば、上記フィルタとそれを納める筒部材（フィルタホルダ２１）との組み付けが不適切なことによる両者間の不適切な隙間による不良を検知できる。また、本発明によれば、上記フィルタにかける圧力を設定できるので、上記不良を確実に検知できる。   According to the present invention, it is possible to detect a defect (typically, the presence or absence of breakage, chipping, gaps, etc.) of a filter (typically, a genome purification filter) made of a porous material that can adsorb the genome in lysed cells. Further, according to the present invention, it is possible to detect a defect due to an inappropriate gap between the two due to improper assembly of the filter and the cylindrical member (filter holder 21) for housing the filter. In addition, according to the present invention, since the pressure applied to the filter can be set, the defect can be reliably detected.

圧力検査装置の概略構成図Schematic configuration diagram of pressure inspection device フィルタ加圧部を示す図The figure which shows a filter pressurization part フィルタを加圧する際の動作を説明するための図。The figure for demonstrating the operation | movement at the time of pressurizing a filter. （a）フィルタホルダとそれに組み込まれたフィルタを示す図、（ｂ）フィルタホルダを側面からみた図、（ｃ）フィルタホルダを上部（上側開口部）からみた図(A) The figure which shows the filter holder and the filter incorporated in it, (b) The figure which looked at the filter holder from the side, (c) The figure which looked at the filter holder from the upper part (upper side opening part) フィルタホルダに純水が分注されている様子を示す図Diagram showing how pure water is dispensed to the filter holder 検証結果１を示す図Diagram showing verification result 1 検証結果２を示す図Diagram showing verification result 2

以下、図面を参照して本発明に係る注水圧力検査装置の一実施形態を説明する。   Hereinafter, an embodiment of a water injection pressure inspection apparatus according to the present invention will be described with reference to the drawings.

図１に、本実施形態に係る圧力検査装置の概略構成図を示す。図１に示すように、本実施形態の圧力検査装置は、コンプレッサー１００と、圧力コントローラー２００と、流量調整器３００と、流量センサ４００と、圧力センサ５００と、フィルタ加圧部６００と、これらを繋ぐ配管とを含む。コンプレッサー１００から、圧縮された空気層が白抜き矢印で示す通りに配管内を流れる。まず、コンプレッサー１００によって圧縮された空気層は、圧力コントローラー２００により１００［ｋＰａ］に制限される。また、最下流であるフィルタ加圧部６００における配管の出口において、空気層が抵抗無く流れている状態で、流量センサ４００にて流量が２６５［ｍＬ／ｍｉｎ］になるよう流量調整器にて調整することにより、フィルタ加圧部６００手前の圧力センサ５００のモニタには０［ｋＰａ］が示される。   In FIG. 1, the schematic block diagram of the pressure inspection apparatus which concerns on this embodiment is shown. As shown in FIG. 1, the pressure inspection apparatus of this embodiment includes a compressor 100, a pressure controller 200, a flow rate regulator 300, a flow rate sensor 400, a pressure sensor 500, a filter pressurizing unit 600, and these. Including connecting piping. From the compressor 100, the compressed air layer flows through the piping as indicated by the white arrows. First, the air layer compressed by the compressor 100 is limited to 100 [kPa] by the pressure controller 200. In addition, the flow rate sensor 400 adjusts the flow rate to 265 [mL / min] with the flow rate sensor 400 in the state where the air layer flows without resistance at the outlet of the piping in the filter pressurizing unit 600 which is the most downstream. Thus, 0 [kPa] is indicated on the monitor of the pressure sensor 500 in front of the filter pressurizing unit 600.

図１の最下流がフィルタ加圧部６００であるが、そのフィルタ加圧部６００の構成を図２に示す。図２に示すように、フィルタ加圧部６００は、フィルタ加圧ヘッド１、加重用ステージ２、スライダ３、Ｌ字支柱４、土台５を含む。   The most downstream side of FIG. 1 is the filter pressurizing unit 600, and the configuration of the filter pressurizing unit 600 is shown in FIG. As shown in FIG. 2, the filter pressurization unit 600 includes a filter pressurization head 1, a weighting stage 2, a slider 3, an L-shaped column 4, and a base 5.

図４（a）に、フィルタホルダ２１に、材質がガラス等で構成されているフィルタ２３ならびにフィルタ２３を支える部材２４が組み込まれている状態（断面形状）を示す。フィルタホルダ２１の先端部２２を下向きとし、入り口側である開口部が大きい方から空気層による圧力をかける。また、フィルタ２３ならびにフィルタ２３を支える部材２４の設置箇所は、フィルタホルダ２１の底部に近い、フィルタ２３の外径と同等の内径の設置箇所に取り付ける。   FIG. 4A shows a state (cross-sectional shape) in which a filter 23 made of glass or the like and a member 24 supporting the filter 23 are incorporated in the filter holder 21. With the tip 22 of the filter holder 21 facing downward, pressure from the air layer is applied from the larger opening on the entrance side. Moreover, the installation location of the filter 23 and the member 24 that supports the filter 23 is attached to an installation location that is close to the bottom of the filter holder 21 and has an inner diameter equivalent to the outer diameter of the filter 23.

フィルタ２３を構成するフィルタ材としては、ゲノム（全染色体を構成するＤＮＡの全塩基配列）を吸着・脱着することが可能で、ゲノム抽出、洗浄等の薬品に耐性のある多孔質な物質（多孔質材料）を使用することができ、具体的にはガラス繊維等が挙げられる。   As a filter material constituting the filter 23, it is possible to adsorb and desorb a genome (all base sequences of DNA constituting all chromosomes), and is a porous substance (porous) resistant to chemicals such as genome extraction and washing. Material) can be used, and specific examples include glass fiber.

フィルタホルダ２１の具体的形状（断面形状）を図４（ｂ）に示す。フィルタホルダ２１は、フィルタ２３を適切に固定することが可能であれば良い。具体的には、図４（ｂ）に示すように、フィルタホルダ２１の本体底部に支持部２６を形成する。図４（ｃ）にフィルタホルダ２１の開口部上部からみた様子を示している。図４（ｃ）に示すように、フィルタ２３を固定する支持部２６は、４箇所に設けられており、これによりフィルタ２３を支える。また、材質がガラス等（多孔質）で構成されているフィルタ２３を設置する前に、フィルタ２３を面全体にて支えるための部材２４を、フィルタホルダ２１の本体底部の支持部２６に取り付けることにより、フィルタ２３が上部からの空気層による圧力をかけても、フィルタ２３は変形等せずフィルタ２３に均一に圧力をかけることが可能となる。   A specific shape (cross-sectional shape) of the filter holder 21 is shown in FIG. The filter holder 21 only needs to be able to fix the filter 23 appropriately. Specifically, as shown in FIG. 4B, a support portion 26 is formed on the bottom of the main body of the filter holder 21. FIG. 4C shows a state seen from the upper part of the opening of the filter holder 21. As shown in FIG. 4C, support portions 26 that fix the filter 23 are provided at four locations, thereby supporting the filter 23. Further, before installing the filter 23 made of glass or the like (porous), a member 24 for supporting the filter 23 over the entire surface is attached to the support portion 26 at the bottom of the main body of the filter holder 21. Thus, even when the filter 23 applies pressure by the air layer from above, the filter 23 is not deformed and the pressure can be uniformly applied to the filter 23.

フィルタ２３を支えるための部材２４としては、剛性が高く、気孔径が大きく空気層や液体等が非常に通過し易いものがよく、例えばＰＰ材やメッシュ材のものが適当である。   As the member 24 for supporting the filter 23, a member having a high rigidity, a large pore diameter, and an air layer, a liquid or the like can be easily passed. For example, a PP material or a mesh material is suitable.

本実施形態によれば、フィルタ２３の破れや欠け等の不良を検出できるので、フィルタ２３の良否を判定できる。また、本実施形態によれば、フィルタ２３を組み付けた状態におけるフィルタホルダ２３（図４（ａ）参照）において、適正な組み付け状態となっていない等の理由で生じたフィルタ２３外径とフィルタホルダ２１内壁との間の不適切な隙間等による不良の有無を判定できる。   According to the present embodiment, it is possible to detect defects such as breakage or chipping of the filter 23, so that the quality of the filter 23 can be determined. Further, according to the present embodiment, the outer diameter of the filter 23 and the filter holder generated due to the reason that the filter holder 23 (see FIG. 4A) in the assembled state is not properly assembled. The presence or absence of a defect due to an inappropriate gap between the inner wall 21 and the like can be determined.

図２のフィルタ加圧ヘッド１は、図４のフィルタホルダ２１の上側の開口部全周を全て覆うようにフィルタホルダ２１の当該開口部の径より少し大きな径にて構成されている。また、フィルタ加圧ヘッド１の先端部にはドーナツ形状のゴムシートが取り付けられており、フィルタホルダ２１の上側の開口部全周と密着して接触することができる。さらに密着度を高めるために、図２に示すように、フィルタ加圧ヘッド１の上部には加重用ステージ２が設けられている。Ｌ字支柱４にスライダ３がネジ接続されており、前記フィルタ加圧ヘッド１ならびに加重用ステージ２が、スライダ３に接続されて上下する機構となっている。また、Ｌ字支柱４が、高さのある土台５に接続することで、検査対象物のセットが容易に行える。   The filter pressurizing head 1 of FIG. 2 is configured with a diameter slightly larger than the diameter of the opening of the filter holder 21 so as to cover the entire circumference of the upper opening of the filter holder 21 of FIG. A donut-shaped rubber sheet is attached to the tip of the filter pressurizing head 1, and can be brought into close contact with the entire circumference of the upper opening of the filter holder 21. In order to further increase the degree of adhesion, as shown in FIG. 2, a weighting stage 2 is provided above the filter pressure head 1. A slider 3 is screwed to the L-shaped support column 4, and the filter pressure head 1 and the weighting stage 2 are connected to the slider 3 to move up and down. In addition, the inspection object can be easily set by connecting the L-shaped column 4 to the base 5 having a height.

図３には、実際に検査を行うときのフィルタ加圧部６００の動作を示す。図３に示すように、フィルタ加圧ヘッド１ならびに加重用ステージ２は、スライダ３の動きに従って、フィルタ加圧ヘッド１の真下に設置されている検査対象物１１に接触するまで下がる。検査対象物１１とは、フィルタ２３が組み込まれたフィルタホルダ２１である（図４（ａ）参照）。その後、加重用ステージ２に２［ｋｇ］以上の重り１２を載せることで、フィルタ加圧ヘッド１の先端に加工されているゴムシートとフィルタホルダ２１（検査対象物１１）の上側の開口部全周と密着して接触することができ、圧縮された空気層が前記接続箇所から漏れることが防止され、フィルタ２３の全面に圧力を適切に掛けることが可能となる。どの程度の重りを使用するかは、検査対象のフィルタホルダ２１とフィルタ加圧ヘッド１との密着性から、適宜決定することができる。   FIG. 3 shows the operation of the filter pressurizing unit 600 when the inspection is actually performed. As shown in FIG. 3, the filter pressurizing head 1 and the weighting stage 2 are lowered according to the movement of the slider 3 until they come into contact with the inspection object 11 installed just below the filter pressurizing head 1. The inspection object 11 is a filter holder 21 in which a filter 23 is incorporated (see FIG. 4A). Thereafter, by placing a weight 12 of 2 [kg] or more on the weighting stage 2, the rubber sheet processed at the tip of the filter pressurizing head 1 and the entire opening on the upper side of the filter holder 21 (inspection object 11). It is possible to make close contact with the circumference, prevent the compressed air layer from leaking from the connection portion, and appropriately apply pressure to the entire surface of the filter 23. How much weight is used can be appropriately determined from the adhesion between the filter holder 21 to be inspected and the filter pressure head 1.

また、上記検査実施の際には、図５に示すように、フィルタホルダ２１の上側の開口部より純水３１を分注し、フィルタ２３を濡らした状態で実施する。このような手順で検査を実施することで、フィルタ２３において、圧力値にて良品ならびに、フィルタ全面ならびに端部における不良品（破れ、欠け、隙間の有無等）を検知することが可能になる。   Further, when performing the inspection, as shown in FIG. 5, pure water 31 is dispensed from the upper opening of the filter holder 21 and the filter 23 is wetted. By performing the inspection in such a procedure, it becomes possible to detect the non-defective product and the defective product (the presence or absence of tears, chips, gaps, etc.) on the entire surface and end of the filter by the pressure value.

以上に説明した検査の検証結果の一例を図６に示す。図６の検証では、図５に示した状態（つまり、フィルタ２３を濡らした状態）のフィルタホルダ２１を検証対象として２９個使用した。ここで、これらのフィルタホルダ２１のうち、番号１〜１０、１２〜２７、２９のフィルタホルダ２１が良品であり、番号１１及び２８のフィルタホルダ２１が不良品である。なお、ここで言う不良品とは、フィルタ２３自体が不良（つまり、破れや欠け等による不良）である場合と、フィルタ２３とフィルタホルダ２１との組み付け状態が不適切であるために生じたフィルタ２３外径とフィルタホルダ２１内壁との間の不適切な隙間による組み付け不良の場合とを含む。また、ここで言う良品とは、フィルタ２３自体が良品であって、又、上記組み付け状態も適切である場合をいう。   An example of the verification result of the inspection described above is shown in FIG. In the verification of FIG. 6, 29 filter holders 21 in the state shown in FIG. 5 (that is, the state in which the filter 23 is wet) are used as verification targets. Here, among these filter holders 21, the filter holders 21 of numbers 1 to 10, 12 to 27, and 29 are non-defective products, and the filter holders 21 of numbers 11 and 28 are defective products. The defective product referred to here is a filter produced because the filter 23 itself is defective (that is, defective due to tearing, chipping, etc.) and the assembled state between the filter 23 and the filter holder 21 is inappropriate. 23 and a case of poor assembly due to an inappropriate gap between the outer diameter of the filter holder 21 and the inner wall of the filter holder 21. The non-defective product referred to here is a case where the filter 23 itself is a non-defective product, and the assembled state is also appropriate.

そして、図１に示す圧力コントローラー２００にて、当該圧力コントローラー２００の後段側の設定圧を５０［ｋＰａ］と１００［ｋＰａ］の二つの条件を設定して、それぞれ検証を実施した。図６に示すように、フィルタホルダ２１の良品と不良品とで、フィルタ加圧ヘッド１によって加圧されたフィルタホルダ２１の上側開口部の圧力（つまり、圧力センサ５００によって測定されたフィルタ２３上側の圧力：以下、測定圧力値という）の差が明確なことが確認できる。具体的には、上記設定圧が１００［ｋＰａ］の場合、良品の測定圧力値が約４８［ｋＰａ］であるのに対して、不良品である番号１１のフィルタホルダ２１の測定圧力値は約１４［ｋＰａ］となっている。また、上記設定圧が５０［ｋＰａ］の場合、良品の測定圧力値が約２９［ｋＰａ］であるのに対して、不良品である番号１１のフィルタホルダ２１の測定圧力値は約６［ｋＰａ］となっている。また、上記設定圧が１００［ｋＰａ］の場合、不良品である番号２８のフィルタホルダ２１の測定圧力値は約３１［ｋＰａ］となっており、上記設定圧が５０［ｋＰａ］の場合、不良品である番号２８のフィルタホルダ２１の測定圧力値は約２７［ｋＰａ］となっている。ここで、不良品である番号２８のフィルタホルダ２１における上記設定圧が１００［ｋＰａ］の場合の測定圧力値と上記設定圧が５０［ｋＰａ］の場合の測定圧力値とから解るように、設定圧が高い１００［ｋＰａ］の方が、より不良品の検知精度が高いことが確認できた。   Then, with the pressure controller 200 shown in FIG. 1, two conditions of 50 [kPa] and 100 [kPa] were set as the set pressure on the subsequent stage of the pressure controller 200, and verification was performed. As shown in FIG. 6, the pressure of the upper opening of the filter holder 21 pressurized by the filter pressurizing head 1 between the non-defective product and the defective product of the filter holder 21 (that is, the upper side of the filter 23 measured by the pressure sensor 500). It can be confirmed that there is a clear difference in pressure (hereinafter referred to as measured pressure value). Specifically, when the set pressure is 100 [kPa], the measured pressure value of the non-defective product is about 48 [kPa], whereas the measured pressure value of the filter holder 21 of No. 11 which is a defective product is about 14 [kPa]. Further, when the set pressure is 50 [kPa], the measured pressure value of the non-defective product is about 29 [kPa], whereas the measured pressure value of the filter holder 21 of No. 11 which is a defective product is about 6 [kPa]. ]. Further, when the set pressure is 100 [kPa], the measured pressure value of the filter holder 21 of number 28, which is a defective product, is about 31 [kPa], and when the set pressure is 50 [kPa], the measured pressure value is not good. The measured pressure value of the non-defective filter holder 21 of No. 28 is about 27 [kPa]. Here, it is set so as to be understood from the measured pressure value when the set pressure is 100 [kPa] and the measured pressure value when the set pressure is 50 [kPa] in the filter holder 21 of number 28 which is a defective product. It was confirmed that the higher the pressure was 100 [kPa], the higher the detection accuracy of defective products.

ここで、図５に示す純水３１を分注せずに検査を実施した場合の検証結果の一例について図７に示す。図７の検証では、図６の検証に対して、図４（ａ）に示した状態（つまり、フィルタ２３を濡らさない状態）のフィルタホルダ２１を検証対象として１４個使用した。ここで、これらのフィルタホルダ２１のうち、番号１〜１０、１２〜１４のフィルタホルダ２１が良品であり、番号１１のフィルタホルダ２１が不良品である。図７から解るように、フィルタ２３を濡らさない状態で検査を実施した場合、フィルタホルダ２１の良品と不良品とで測定圧力値は殆ど差が無く、良品、不良品に関わらず殆ど上昇しないことが確認できる。   Here, FIG. 7 shows an example of a verification result when the inspection is performed without dispensing the pure water 31 shown in FIG. In the verification of FIG. 7, 14 filter holders 21 in the state shown in FIG. 4A (that is, the state in which the filter 23 is not wetted) are used as verification targets, compared to the verification of FIG. 6. Here, among these filter holders 21, the filter holders 21 having numbers 1 to 10 and 12 to 14 are non-defective products, and the filter holder 21 having number 11 is a defective product. As can be seen from FIG. 7, when the inspection is carried out in a state where the filter 23 is not wetted, there is almost no difference in the measured pressure value between the non-defective product and the defective product of the filter holder 21, and hardly increases regardless of the good product or the defective product. Can be confirmed.

以上のように、本実施形態によれば、フィルタ２３自体の不良（又は、フィルタ２３とフィルタホルダ２１との組み付け不良）を検出することができる。   As described above, according to the present embodiment, it is possible to detect a defect in the filter 23 itself (or a defective assembly between the filter 23 and the filter holder 21).

上記の本実施形態では、血液の細胞からゲノムを抽出する場合を一例に挙げて説明した。しかし、本実施形態において、血液以外の細胞からゲノムを抽出してもよい。   In the above-described embodiment, the case of extracting a genome from blood cells has been described as an example. However, in this embodiment, the genome may be extracted from cells other than blood.

上記の本実施形態では、フィルタ２３を純水で濡らして検査を行った。しかし、本実施形態において、ゲノム精製（抽出）に支障をきたさない他の液体でフィルタ２３を濡らして検査を行ってもよい。例えば、フィルタ２３のガラス繊維やフィルタホルダ２１（プラスチック）等を傷めない他の液体でフィルタ２３を濡らして検査を行ってもよい。   In the above-described embodiment, the inspection was performed by wetting the filter 23 with pure water. However, in this embodiment, the test may be performed by wetting the filter 23 with another liquid that does not interfere with genome purification (extraction). For example, the inspection may be performed by wetting the filter 23 with another liquid that does not damage the glass fiber of the filter 23, the filter holder 21 (plastic), or the like.

上記の本実施形態では、空気層で加圧することによって検査を行った。しかし、本実施形態において、ゲノム精製（抽出）に支障をきたさない他の気体層（ガス）で加圧することによって検査を行ってもよい。例えば、ガラス繊維やプラスチック類と反応せず、ゲノムを壊さない他の気体層で加圧することによって検査を行ってもよい。   In the present embodiment, the inspection is performed by pressurizing with an air layer. However, in this embodiment, you may test | inspect by pressurizing with the other gas layer (gas) which does not interfere with genome refinement | purification (extraction). For example, you may test | inspect by pressurizing with the other gas layer which does not react with glass fiber or plastics and does not destroy a genome.

本発明は、フィルタ等の品質検査等に関して利用可能であり、特に、血液からゲノムを精製するために使用されるフィルタの品質検査等に有用である。   The present invention can be used for quality inspection of filters and the like, and is particularly useful for quality inspection of filters used for purifying genome from blood.

１・・・フィルタ加圧ヘッド
２・・・加重用ステージ
３・・・スライダ
４・・・Ｌ字支柱
５・・・土台
１１・・・検査対象物
１２・・・重り
２１・・・フィルタホルダ
２２・・・フィルタホルダ先端部
２３・・・フィルタ
３１・・・純水
１００・・・コンプレッサー
２００・・・圧力コントローラー
３００・・・流量調整記
４００・・・流量センサ
５００・・・圧力センサ
６００・・・フィルタ加圧部 DESCRIPTION OF SYMBOLS 1 ... Filter pressurization head 2 ... Weighting stage 3 ... Slider 4 ... L-shaped support | pillar 5 ... Base 11 ... Inspection object 12 ... Weight 21 ... Filter holder 22 ... Filter holder tip 23 ... Filter 31 ... Pure water 100 ... Compressor 200 ... Pressure controller 300 ... Flow rate adjustment 400 ... Flow rate sensor 500 ... Pressure sensor 600 ... Filter pressurizing part

Claims (8)

溶解された細胞中のゲノムを吸着できる多孔質材料から成り、所定の液体を含んだ状態のフィルタの主面の一方に対して、気体層によって圧力をかける加圧手段と、
前記加圧手段によって圧力がかけられた前記主面の一方における前記気体層の圧力を検出する圧力検出手段とを備える、圧力検査装置。 A pressurizing means made of a porous material capable of adsorbing the lysed genome in the cell, and applying a pressure by a gas layer to one of the main surfaces of the filter containing a predetermined liquid;
A pressure inspection device comprising: pressure detection means for detecting the pressure of the gas layer on one of the main surfaces to which pressure is applied by the pressure means. 前記加圧手段は、前記フィルタが組み付けられることによって閉塞された筒部材の一方の開放端側に対して圧力をかけることによって、当該フィルタの主面の一方に対して圧力をかける、請求項１に記載の圧力検査装置。   The pressure means applies pressure to one of the main surfaces of the filter by applying pressure to one open end side of the cylindrical member closed by assembling the filter. The pressure inspection device described in 1. 前記加圧手段によって前記フィルタの主面の一方に対して圧力をかけるための前記気体層の流量を検出する流量検出手段と、
前記気体層の流量を調整する流量調整手段とを備える、請求項１又は２に記載の圧力検査装置。 A flow rate detecting means for detecting a flow rate of the gas layer for applying pressure to one of the main surfaces of the filter by the pressurizing means;
The pressure inspection apparatus according to claim 1, further comprising a flow rate adjusting unit that adjusts a flow rate of the gas layer. 前記加圧手段によって前記フィルタの主面の一方に対してかけられる圧力を設定する圧力設定手段を備える、請求項１〜３のいずれかに記載の圧力検査装置。   The pressure test | inspection apparatus in any one of Claims 1-3 provided with the pressure setting means which sets the pressure applied with respect to one of the main surfaces of the said filter by the said pressurization means. 溶解された細胞中のゲノムを吸着できる多孔質材料から成り、所定の液体を含んだ状態のフィルタの主面の一方に対して、気体層によって圧力をかける加圧ステップと、
前記加圧ステップによって圧力がかけられた前記主面の一方における前記気体層の圧力を検出する圧力検出ステップとを備える、圧力検査方法。 A pressurizing step of applying a pressure by a gas layer to one of the main surfaces of the filter made of a porous material capable of adsorbing the lysed cell genome and containing a predetermined liquid;
And a pressure detecting step of detecting a pressure of the gas layer on one of the main surfaces to which pressure is applied by the pressurizing step. 前記加圧ステップでは、前記フィルタが組み付けられることによって閉塞された筒部材の一方の開放端側に対して圧力をかけることによって、当該フィルタの主面の一方に対して圧力をかける、請求項５に記載の圧力検査方法。   6. In the pressurizing step, pressure is applied to one of the main surfaces of the filter by applying pressure to one open end side of the cylindrical member closed by assembling the filter. The pressure inspection method described in 1. 前記加圧ステップによって前記フィルタの主面の一方に対して圧力をかけるための前記気体層の流量を検出する流量検出ステップと、
前記気体層の流量を調整する流量調整ステップとを備える、請求項５又は６に記載の圧力検査方法。 A flow rate detecting step for detecting a flow rate of the gas layer for applying pressure to one of the main surfaces of the filter by the pressurizing step;
The pressure inspection method according to claim 5, further comprising a flow rate adjustment step of adjusting a flow rate of the gas layer. 前記加圧ステップによって前記フィルタの主面の一方に対してかけられる圧力を設定する圧力設定ステップを備える、請求項５〜７のいずれかに記載の圧力検査方法。   The pressure inspection method according to claim 5, further comprising a pressure setting step for setting a pressure applied to one of the main surfaces of the filter by the pressurizing step.
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