JP2013027378A - Separation method and separation system of microalgae - Google Patents

Separation method and separation system of microalgae Download PDF

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JP2013027378A
JP2013027378A JP2011167424A JP2011167424A JP2013027378A JP 2013027378 A JP2013027378 A JP 2013027378A JP 2011167424 A JP2011167424 A JP 2011167424A JP 2011167424 A JP2011167424 A JP 2011167424A JP 2013027378 A JP2013027378 A JP 2013027378A
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Hiroshi Tanaka
浩 田中
Katsuaki Matsuzawa
克明 松澤
Kosuke Ishii
浩介 石井
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Abstract

PROBLEM TO BE SOLVED: To provide a separation method in which microalgae can be separated from a medium with purity and by good productivity, and a system of the same.SOLUTION: A separation technique of the microalgae in which a medium including the microalgae is sequentially applied to two or more separators 10, 12, and 14 with mutually different pore diameters, thereby the microalgae and a foreign substance are fractionated according to the size is disclosed, and especially the colony of the microalgae is fractionated from the medium-small foreign substances and the large-scale foreign substance.

Description

本発明は微細藻類を培地（培養液）から純粋かつ収率よく分離する方法及びそのシステムに関するものである。   The present invention relates to a method and system for separating microalgae from a medium (culture medium) in a pure and high yield.

バイオマスが豊富である微細藻類からバイオ燃料を生産することが古くから着目されている。そして、近年では、バイオ燃料の大量生産のための技術開発が盛んに進められている。技術開発の主眼の一に、微細藻類を安価に大規模生産することがある。   Producing biofuels from microalgae rich in biomass has long attracted attention. In recent years, technological development for mass production of biofuels has been actively promoted. One of the main focus of technological development is the large-scale production of microalgae at a low cost.

従来、微細藻類の大量培養技術は、健康食品、医薬など付加価値の高い生理活性物質の商業生産を目的に開発されてきた。その一つとして、微細藻類の培養パネルを垂直に置き、水平方向から光を照射することにより、藻類を高密度で高速に増殖させることが行われてきた。   Conventionally, a technique for mass culture of microalgae has been developed for the purpose of commercial production of highly active physiologically active substances such as health foods and pharmaceuticals. As one of them, algae can be grown at high density and at high speed by placing a culture panel of microalgae vertically and irradiating light from the horizontal direction.

しかしながら、生理活性物質よりも単位重量当りの対価が低いバイオ燃料の製造では、既述の方式では、装置の稼働に要するエネルギーの消費量が大きく採算が合わないため、野外の開放系装置における藻類の大量培養方式が行われている。   However, in the production of biofuel, which has a lower price per unit weight than bioactive substances, the energy consumption required for the operation of the device is large and unprofitable with the above-described method. A large-scale culture method is used.

藻類を野外で大量培養した後バイオマスを回収するためには、固形分としての微細藻類と液体成分としての培地とを分離して、先ず、微細藻類を凝縮する。次いで、ボールミルやホモジナイザーで微細藻類を破壊してバイオ燃料を藻体の外に取り出した後、破砕物を脱水乾燥する。脱水乾燥物からバイオ燃料を取り出すためには、ヘキサン等の極性溶媒や超臨界油媒を用いて、脱水乾燥物からバイオ燃料を抽出する。   In order to collect biomass after algae are cultured in large quantities in the field, the microalgae as a solid component and the medium as a liquid component are separated, and the microalgae are first condensed. Next, the microalgae are destroyed with a ball mill or a homogenizer, and the biofuel is taken out of the alga body, and then the crushed material is dehydrated and dried. In order to extract biofuel from the dehydrated dried product, the biofuel is extracted from the dehydrated dried product using a polar solvent such as hexane or a supercritical oil medium.

特表２０１０−５１５４４１号公報には、藻類を培養後、培養液から遠心分離によって藻類を凝縮し、次いで、バイオ燃料を得ることが記載されている。培養液から藻類を分離する他の形態としては、フィルタを用いる形態もある。   Japanese Patent Application Publication No. 2010-515441 describes that after culturing algae, the algae is condensed from the culture solution by centrifugation, and then a biofuel is obtained. Another form for separating algae from the culture solution is a form using a filter.

特表２０１０−５１５４４１号公報Special table 2010-515441

既述の分離方法は、液体成分（培地）と固体成分（藻体）との分離を目的としているが、固体成分が目的の藻体だけであるとは限らない。すなわち、固体成分の中には、不要なバクテリアや大気中から混入したゴミや昆虫、大型藻類の切れ端等の異物が含まれていることが多い。このため、遠心分離法では、固体成分の中には、微細藻類の中に大型の異物が混入するおそれがある。これらの異物がそのまま次の脱水・乾燥工程に送られると、バイオ燃料等の最終製品の品質が低下する問題がある。これを避けるためには、最終製品から異物を除去するための工程や装置が別に必要になる。   The above-described separation method is intended to separate the liquid component (medium) and the solid component (algae), but the solid component is not necessarily the target alga body. That is, the solid component often contains foreign substances such as unwanted bacteria, dust and insects mixed from the atmosphere, and fragments of large algae. For this reason, in the centrifugal separation method, a large foreign substance may be mixed in the microalgae in the solid component. When these foreign substances are sent to the next dehydration / drying process as they are, there is a problem that the quality of the final product such as biofuel is deteriorated. In order to avoid this, a separate process or apparatus for removing foreign substances from the final product is required.

また、フィルタを用いた分離法では、大型異物を除くことができる半面、バクテリアなどの小型異物を除くことができない。さらに、フィルタを用いた分離法では、微細藻類の群体（コロニー）も異物と一緒に取り除かれてしまうおそれがあり、最終製品の収率を低下させるおそれがある。   Moreover, the separation method using a filter can remove large foreign matters, but cannot remove small foreign matters such as bacteria. Furthermore, in the separation method using a filter, microalgae colonies may be removed together with foreign substances, which may reduce the yield of the final product.

そこで、本発明は、微細藻類を培地から純粋かつ収率よく分離できる分離方法及びそのシステムを提供することを目的とする。   Then, an object of this invention is to provide the isolation | separation method and its system which can isolate | separate a micro algae from a culture medium purely and with a sufficient yield.

本発明者は、前記目的を達成するために、バイオ燃料などの最終有用物を得るための微細藻類として、コロニー形成能を有する微細藻類に着目した。この微細藻類が形成するコロニーの直径サイズは３０μｍ〜３００μｍ（平均１００μｍ程度）である。このサイズは、バクテリア等を含む小型異物（１〜５０μｍ程度）、ゴミ、昆虫等の大型異物（１ｍｍ以上）とオーダー単位で異なるため、細孔径が互いに異なる複数の分離手段を組み合わせることにより、これら成分を互いに分画することができる。   In order to achieve the above object, the present inventor has focused on microalgae having colony-forming ability as microalgae for obtaining final useful substances such as biofuels. The diameter size of colonies formed by these microalgae is 30 μm to 300 μm (average of about 100 μm). This size differs from small foreign matter containing bacteria (about 1 to 50 μm), large foreign matter such as dust and insects (1 mm or more) in order units, so by combining multiple separation means with different pore diameters, The components can be fractionated from each other.

すなわち、本発明は、微細藻類を含む培地を互いに異なる細孔径を持つ複数の分離手段に順番に適用することにより、前記微細藻類と異物とをサイズ別に分画する微細藻類の分離方法であり、特に、微細藻類のコロニーを、前記中小の異物及び大型異物とから分画することを特徴としている。   That is, the present invention is a method for separating microalgae by fractionating the microalgae and foreign substances according to size by sequentially applying a medium containing microalgae to a plurality of separation means having different pore sizes, In particular, the present invention is characterized in that microalgae colonies are fractionated from the small and medium foreign substances and the large foreign substances.

一方、本発明に係る微細藻類の分離システムは、互いに異なる細孔径を持つ複数の分離手段を備え、微細藻類のコロニーを含む培養液を順番に処理して、前記コロニーと異物とをサイズ別に分画することを特徴とするものである。   On the other hand, the microalgae separation system according to the present invention includes a plurality of separation means having different pore sizes, and sequentially processes a culture solution containing colonies of microalgae to separate the colonies and foreign substances according to size. It is characterized by drawing.

本発明の対象となる微細藻類は、コロニー形成能を持ちながらバイオ燃料を生成可能なものであればよく、特に限定されるべきものでないが、例えば、ボツリオコッカス属に属するものが好適である。ボツリオコッカス属に属する株とし、Botryococcus braunii Kutzing (株番号２１９９)、Botryococcus braunii Kutzing （株番号８３６）が知られている。これらの株は、茨城県つくば市小野川16-2所在の（独）国立環境研究所微生物系統保存施設から入手することができる。   The microalgae that is the subject of the present invention is not particularly limited as long as it has a colony-forming ability and can generate biofuel. For example, those belonging to the genus Botulococcus are preferable. . Botryococcus braunii Kutzing (strain number 2199) and Botryococcus braunii Kutzing (strain number 836) are known as strains belonging to the genus Botriococcus. These strains can be obtained from the National Institute for Environmental Studies microbial strain storage facility, 16-2 Onagawa, Tsukuba City, Ibaraki Prefecture.

複数の分離手段は、大型異物と微細藻類のコロニーとを分画するための第１のフィルタを有する第１ユニットと、小型異物と微細藻類のコロニーとを分画するための第２のフィルタを有する第２ユニットと、から構成される。第１の分離手段としての第１ユニットは、培地中の微細藻類のコロニーを含む固体成分を、大型異物からなる第１の画分と、微細藻類のコロニーからなる第２の画分と、に分離し、第２の分離手段としての第２ユニットは、小型異物からなる第３の画分を分離する。   The plurality of separation means includes a first unit having a first filter for fractionating large foreign matter and microalgae colonies, and a second filter for fractionating small foreign matter and microalgae colonies. And a second unit. The first unit as the first separation means includes a solid component containing a microalgae colony in the medium into a first fraction composed of large foreign substances and a second fraction composed of microalgae colonies. The second unit as the second separating means separates the third fraction composed of small foreign matters.

第１のフィルタは、微細藻類のコロニーが通過でき、大型異物が通過できない、ポアサイズを有する。第２のフィルタは、小型異物が通過でき、微細藻類のコロニーが通過できないポアサイズを有する。   The first filter has a pore size through which colonies of microalgae can pass and large foreign substances cannot pass. The second filter has a pore size through which small foreign substances can pass and microalgae colonies cannot pass through.

本発明の分離方法は、液体成分と小型異物以上の個体成分とを分離するための第３のフィルタを有する第３ユニット（第３の分離手段）を備えてもよい。第３のフィルタは、小型異物を通過しない超微細なポアサイズを有する。第３のフィルタによって固体成分が分離された液体成分はバクテリアを含まないろ過水であるため、微細藻類の培地用の水や逆洗水として再利用することができる。その際、水中に含まれるコロイド状物質をＰＡＣや硫酸バンド等の凝集剤を適用して沈殿させて除けばよい。   The separation method of the present invention may include a third unit (third separation means) having a third filter for separating a liquid component and an individual component that is not less than a small foreign object. The third filter has an extremely fine pore size that does not pass through a small foreign matter. Since the liquid component from which the solid component has been separated by the third filter is filtered water containing no bacteria, it can be reused as water for microalgae culture medium or backwash water. At that time, the colloidal substance contained in the water may be removed by applying a flocculant such as PAC or sulfuric acid band.

小型異物の中には、微細藻類の単細胞や、微細藻類のコロニーの断片、あるいは小型コロニーが含まれている可能性も高い。そこで、小型異物を廃棄する前に小型異物を種にして予備的な培養を行い、微細藻類のコロニーが増殖する場合には小型異物の培養液を破棄することなく、そのまま本格培養を継続し、培養液をバイオ燃料用の原料として再利用することができる。予備的培養によって、微細藻類のコロニーが確認できない場合には、小型異物は藻類以外のバクテリアを含む可能性が高いため、小型異物を含むろ過水を破棄する。予備的な培養とは、本格培養のための培養池ではなく、予備的な培養池等で行う比較的短期間の培養方法である。小型異物を微細藻類のコロニーの培養に利用することによって、微細藻類の培養を効率良く進めることができる。   There is a high possibility that small foreign substances include single cells of microalgae, fragments of colonies of microalgae, or small colonies. Therefore, before discarding the small foreign matter, perform a preliminary culture using the small foreign matter as a seed, and if the microalgae colonies grow, continue the full-scale cultivation without discarding the culture solution of the small foreign matter, The culture solution can be reused as a raw material for biofuel. If microalgae colonies cannot be confirmed by preliminary culture, small foreign substances are likely to contain bacteria other than algae, and the filtered water containing small foreign substances is discarded. Preliminary culture is a relatively short-term culture method performed in a preliminary culture pond or the like, not a culture pond for full-scale culture. By using the small foreign matter for culturing the microalgae colonies, the microalgae can be cultivated efficiently.

本発明によれば、微細藻類を培地から純粋かつ収率よく分離できる分離方法及びそのシステムを提供することができる。   ADVANTAGE OF THE INVENTION According to this invention, the isolation | separation method and its system which can isolate | separate a micro algae from a culture medium purely with a sufficient yield can be provided.

本発明に係る微細藻類の分離システムの一形態を示すブロック図である。It is a block diagram which shows one form of the separation system of the micro algae concerning this invention.

次に、本発明の実施形態について説明する。図１は微細藻類の分離システムの一形態を示すブロック図である。微細藻類の分離システムは、第１のフィルタを有する第１ユニット１０と、第２のフィルタを有する第２ユニット１２と、第３のフィルタを有する第３ユニット１４とをこの順に連結させた構成を備えている。   Next, an embodiment of the present invention will be described. FIG. 1 is a block diagram showing an embodiment of a microalgae separation system. The microalgae separation system has a configuration in which a first unit 10 having a first filter, a second unit 12 having a second filter, and a third unit 14 having a third filter are connected in this order. I have.

第１ユニット１０は、例えば、第１のフィルタを備えるドラムスクリーン装置から構成され、第２ユニット１２も同様に第２のフィルタを備えるドラムスクリーン装置から構成される。第３ユニット１４の第３のフィルタは超微細なポアサイズを有するために、ドラムスクリーン装置よりも例えば中空糸からなる精密ろ過膜のユニットから構成される。   For example, the first unit 10 is configured by a drum screen device including a first filter, and the second unit 12 is also configured by a drum screen device including a second filter. Since the third filter of the third unit 14 has an extremely fine pore size, it is constituted by a microfiltration membrane unit made of, for example, a hollow fiber rather than a drum screen device.

第１のフィルタは、微細藻類のコロニーが通過でき、大型異物が通過できない、第１のポアサイズを有する。第１のポアサイズは、例えば、０．１〜１ｍｍである。第１のフィルタは、例えば、第１のポアサイズを有する金属製スクリーンから構成される。   The first filter has a first pore size through which microalgae colonies can pass and large foreign objects cannot pass. The first pore size is, for example, 0.1 to 1 mm. The first filter is formed of, for example, a metal screen having a first pore size.

第２のフィルタは、小型異物が通過でき、微細藻類のコロニーが通過できない第２のポアサイズを有する。第２のポアサイズは、例えば、２０〜５０μｍである。２０μｍ未満では、夾雑物としての種々のバクテリアを十分除けないおそれがある。一方、５０μｍを越えると、微細藻類のコロニーのうち小型のものが異物として除かれてしまうおそがある。第２のフィルタは、例えば、第２のポアサイズを有するナイロン製フィルタから構成される。   The second filter has a second pore size through which small foreign substances can pass and microalgae colonies cannot pass through. The second pore size is, for example, 20 to 50 μm. If it is less than 20 μm, there is a possibility that various bacteria as contaminants cannot be sufficiently removed. On the other hand, if it exceeds 50 μm, small ones among microalgae colonies may be removed as foreign substances. The second filter is composed of, for example, a nylon filter having a second pore size.

第３のフィルタは、小型異物を通過しない超微細な第３のポアサイズを有する。第３のポアサイズは、例えば、０．１μｍ程度である。材質は、例えば下水処理工程での膜ろ過で使われているPVDF（ポリフッ化ビニリデン）である。   The third filter has an extremely fine third pore size that does not pass through the small foreign matter. The third pore size is, for example, about 0.1 μm. The material is, for example, PVDF (polyvinylidene fluoride) used in membrane filtration in the sewage treatment process.

次に、分離システムの動作について説明する。培養池からくみ上げられた培養液（培地）Ｓ１は、第３ユニット１４に供給される。第３ユニット１４は、中空糸膜によって、培養液を固体成分（小型異物、コロニー、大型異物）とろ過水Ｓ２に分離する。既述のとおり、液体成分には所定の浄化工程１６を行い、浄化水を培地用の水Ｓ３と逆洗用の水とに利用する。逆洗水Ｇ１は第３ユニット１４に供給され、逆洗水Ｇ１は中空糸膜外に付着した固体成分をブロアとともに剥ぎ取り、固体成分を含む処理水Ｙ１は第２ユニット１２に供給される。なお，Ｇ２は逆洗用の水として第２ユニット１２に供給される。Ｇ３も逆洗用の水として第１ユニット１０に供給される。   Next, the operation of the separation system will be described. The culture solution (medium) S <b> 1 pumped up from the culture pond is supplied to the third unit 14. The 3rd unit 14 isolate | separates a culture solution into a solid component (a small foreign material, a colony, a large foreign material) and filtered water S2 with a hollow fiber membrane. As described above, the liquid component is subjected to the predetermined purification step 16 and the purified water is used as the medium water S3 and the backwash water. The backwash water G1 is supplied to the third unit 14, the backwash water G1 peels off the solid component adhering to the outside of the hollow fiber membrane together with the blower, and the treated water Y1 containing the solid component is supplied to the second unit 12. G2 is supplied to the second unit 12 as backwash water. G3 is also supplied to the first unit 10 as water for backwashing.

第２ユニット１２のロータリドラムには第２のフィルタが巻回されており、第３ユニット１２からの処理水Ｙ１が供給されると、第２のフィルタは既述の小型異物を通過させるため、処理水Ｙ１は、小型異物を含むろ過水Ｒ１と、第２フィルタを通過できないコロニー及び大型異物に分離される。小型異物を含むろ過水Ｒ１は、既述のとおり、予備培養の対象とされる。   The second filter is wound around the rotary drum of the second unit 12, and when the treated water Y1 from the third unit 12 is supplied, the second filter passes the small foreign matter described above. The treated water Y1 is separated into filtered water R1 containing small foreign matter, colonies that cannot pass through the second filter, and large foreign matter. As described above, the filtered water R1 containing small foreign substances is a subject of preliminary culture.

第２ユニット１２には第３ユニット１４からの逆洗水Ｇ２が供給され、第２のフィルタに付着したコロニー及び大型異物からなる固形物はブロアと逆洗水Ｇ２によって逆洗され、固形物を含む処理水Ｙ２は、第１ユニット１０に供給される。   The second unit 12 is supplied with the backwashing water G2 from the third unit 14, and the solid matter consisting of colonies and large foreign matters adhering to the second filter is backwashed with the blower and the backwashing water G2 to remove the solid matter. The treated water Y2 is supplied to the first unit 10.

第１ユニット１０のロータリドラムには第１のフィルタが巻回されており、第２ユニット１２からの処理水Ｙ２が供給されると、第１のフィルタはコロニーを通過させ、大型異物を通過させないため、第１フィルタは処理水Ｙ２を、コロニーを含むろ過水Ｒ２と大型異物に分離する。コロニーを含むろ過水Ｒ２は、遠心分離されて、コロニーの凝集物が収集される。コロニーの凝集物は、バイオ燃料を生成するための次の工程に送られる。   The first filter is wound around the rotary drum of the first unit 10, and when the treated water Y2 from the second unit 12 is supplied, the first filter passes colonies and does not allow large foreign matters to pass. Therefore, the first filter separates the treated water Y2 into filtered water R2 including colonies and large foreign matters. The filtered water R2 containing colonies is centrifuged to collect colony aggregates. The colony agglomerates are sent to the next step to produce biofuel.

第1ユニット１０には第３ユニット１０から逆洗用の水Ｇ３が供給され、第１のフィルタに付着した大型異物からなる固形物はブロアと逆洗水にＧ３よって逆洗され、大型異物を含む処理水Ｙ３は大型異物の廃棄工程１８に送られる。大型異物はサイズが大きく、脱水性に優れるため、天日乾燥などの乾燥工程を経て焼却処分される。   The first unit 10 is supplied with water G3 for backwashing from the third unit 10, and the solid matter consisting of large foreign matter adhering to the first filter is backwashed by the blower and backwashing water with G3 to remove the large foreign matter. The treated water Y3 contained is sent to the large foreign matter disposal step 18. Large foreign substances are large in size and excellent in dewaterability, and are therefore incinerated through a drying process such as sun drying.

以上の工程によって、培養液から微細藻類の分離が終了する。微細藻類のコロニーが存在するろ過水Ｒ２には、大型異物及びバクテリアなどの小異物が含まれず、微細藻類の純粋な分離が行われる。また、小型異物を含むろ過水Ｒ１には微細藻類の細分化されたコロニーが含まれている可能性がある。微細藻類の細分化されたコロニーが予備培養によって確認されると、細分化されたコロニーを用いて微細藻類の本格培養が継続される。したがって、微細藻類の細分化されたコロニーが廃棄されず再利用されるため、微細藻類の収率が向上する。   Through the above steps, the separation of the microalgae from the culture solution is completed. The filtered water R2 in which microalgae colonies exist does not contain large foreign substances and small foreign substances such as bacteria, and the pure algae are separated. Moreover, there is a possibility that the filtered water R1 containing small foreign matters contains finely divided colonies of algae. When the subdivided colonies of the microalgae are confirmed by the preliminary culture, the full-scale culture of the microalgae is continued using the subdivided colonies. Therefore, since the subdivided colonies of microalgae are reused without being discarded, the yield of microalgae is improved.

予備的培養の一例は次のとおである。まず小型異物を含む固体成分を、培地で希釈するか、または第２のフィルタと同等のフィルタで濃縮して、培養中の微細藻類濃度と同等（おおむね１〜１０ｇ乾燥藻体/Ｌ程度）とし、２〜１０日程度培養する。この予備的培養によって、目的の藻体が増殖したかどうかは、顕微鏡で観察するなどして藻体の形態（細胞の形、コロニーの形、色など）から容易に判断できる。   An example of preliminary culture is as follows. First, the solid component containing small foreign substances is diluted with a medium or concentrated with a filter equivalent to the second filter, so that it is equivalent to the concentration of microalgae in culture (approximately 1 to 10 g dry algae / L). Incubate for about 2 to 10 days. Whether or not the target alga body has grown by this preliminary culture can be easily determined from the form of the alga body (cell shape, colony shape, color, etc.) by observing under a microscope.

なお、既述の実施形態では、培養液Ｓ１を最初に精密ろ過膜によって処理したため、ろ過水を第１ユニット１０と第２ユニット１２用の逆洗水として再利用することができる。なお、既述の実施形態では、培養液を第３ユニットに先ず供給し、次いで、第２ユニット、第３ユニットの順で培養液のろ過を進めたが、第１ユニット側から培養液をろ過するものであってもよい。   In the above-described embodiment, since the culture solution S1 is first processed by the microfiltration membrane, the filtered water can be reused as the backwash water for the first unit 10 and the second unit 12. In the above-described embodiment, the culture solution is first supplied to the third unit, and then the culture solution is filtered in the order of the second unit and the third unit. However, the culture solution is filtered from the first unit side. You may do.

１０ 第１ユニット（第１分離手段）
１２ 第２ユニット（第２分離手段）
１４ 第３ユニット（第３分離手段）
Ｓ１ 微細藻類のコロニーを含む培養液
Ｓ２ 異物を含まないろ過水
Ｒ１ 小型異物（バクテリア、再分化されたコロニーなど）を含むろ過水
Ｒ２ 微細藻類のコロニーを含むろ過水 10 First unit (first separating means)
12 Second unit (second separating means)
14 Third unit (third separating means)
S1 Culture solution containing microalgae colonies S2 Filtered water containing no foreign matter R1 Filtered water containing small foreign matter (bacteria, regenerated colonies, etc.) R2 Filtered water containing microalgal colonies

Claims (9)

微細藻類を含む培養液を互いに異なる細孔径を持つ複数の分離手段を順番に通過させる第１の工程と、
前記培養液を前記複数の分離手段を順番に通過させる過程で、前記微細藻類と異物とをサイズ別に順番に分画する第２の工程と、
を含む微細藻類の分離方法。 A first step of sequentially passing a culture solution containing microalgae through a plurality of separation means having different pore sizes;
A second step of fractionating the microalgae and foreign substances in order according to size in the process of sequentially passing the culture solution through the plurality of separation means;
Method for separating microalgae containing 前記第２の工程は、前記微細藻類のコロニーを前記異物から分画する、請求項１記載の微細藻類の分離方法。   The method for separating microalgae according to claim 1, wherein in the second step, the microalgae colonies are fractionated from the foreign substances. 前記分離手段は、
前記コロニーを通過させて、大型異物を通過させない細孔径を持つ第１のフィルタと、
小型異物を通過させ、前記コロニーを通過させない細孔径を持つ第２のフィルタと、
を備え、
前記第２の工程は、
前記第１のフィルタによって、前記大型異物と前記コロニーとを分画し、
前記第２のフィルタによって、前記小型異物を分画する、
請求項２記載の微細藻類の分離方法。 The separating means includes
A first filter having a pore diameter that allows passage of the colonies and does not allow passage of large foreign matter;
A second filter having a pore size that allows passage of small foreign objects and does not allow passage of the colonies;
With
The second step includes
By the first filter, the large foreign matter and the colony are fractionated,
The small foreign matter is fractionated by the second filter.
The method for separating microalgae according to claim 2. 前記分離手段は、前記小型異物を通過させない細孔径を持つ第３のフィルタを備え、
前記第２の工程は、前記第３のフィルタによって前記コロニー、前記小型異物、及び、前記大型異物を含まないろ過水を分画する、請求項１乃至３の何れか１項記載の微細藻類の分離方法。 The separation means includes a third filter having a pore diameter that does not allow the small foreign matter to pass through,
The microalgae according to any one of claims 1 to 3, wherein in the second step, the colony, the small foreign matter, and filtered water not containing the large foreign matter are fractionated by the third filter. Separation method. 前記小型異物を培養して、前記微細藻類のコロニーが成長するか否かを判定する工程を有し、当該コロニーが成長した場合には、前記第１の工程は、前記小型異物の培養液を前記複数の分離手段に適用する、請求項４記載の微細藻類の分離方法。   Incubating the small foreign matter to determine whether or not the microalgae colonies grow, and when the colonies grow, the first step includes culturing the small foreign matter culture solution. The method for separating microalgae according to claim 4, which is applied to the plurality of separation means. 前記微細藻類がボツリオコッカス属のコロニー生産性の株である、請求項１乃至５の何れか１項記載の微細藻類の分離方法。   The method for separating microalgae according to any one of claims 1 to 5, wherein the microalgae is a colony-producing strain of the genus Botriococcus. 前記第２の工程は、前記培養液を、前記第３のフィルタ、次いで、第２のフィルタ、次いで、第１のフィルタの順に通過させる、請求項４記載の微細藻類の分離方法。   The method for separating microalgae according to claim 4, wherein in the second step, the culture solution is passed in the order of the third filter, then the second filter, and then the first filter. 前記ろ過水を前記第１のフィルタ及び第２のフィルタに逆洗用の水として供給する工程を含む、請求項７記載の微細藻類の分離方法。   The method for separating microalgae according to claim 7, comprising a step of supplying the filtered water as backwash water to the first filter and the second filter. 互いに異なる細孔径を持つ複数の分離手段を備え、
当該複数の分離手段は、微細藻類のコロニーを含む培養液を順番に処理して、前記コロニーと異物とをサイズ別に分画する、微細藻類の分離システム。 A plurality of separation means having different pore sizes from each other,
The said several isolation | separation means processes the culture solution containing the colony of a micro algae in order, and fractionates the said colony and a foreign material according to size, The separation system of a micro algae.
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