JP2012522992A - 抗凝固処理血液薄膜試料における特定の貪食作用の測定によるヒト又は動物の感染症の検出 - Google Patents
抗凝固処理血液薄膜試料における特定の貪食作用の測定によるヒト又は動物の感染症の検出 Download PDFInfo
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- 238000000034 method Methods 0.000 claims abstract description 29
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- 238000012512 characterization method Methods 0.000 description 2
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
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- 241000589969 Borreliella burgdorferi Species 0.000 description 1
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- 241000588653 Neisseria Species 0.000 description 1
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
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Abstract
【選択図】図1
Description
Claims (12)
- 抗凝固処理全生血の単層薄膜試料を分析して、血液ドナーの所定の病原体による最近の曝露又は現在の感染の有無を調べる方法であって、標的検体が、特定の単数又は複数の抗原がコーティングされた粒子に対する、血液試料に含まれる白血球による特定の貪食作用及び/又は結合作用の有無である方法において、
前記血液試料を、前記標的検体に特異的な抗原がコーティングされた第1の検出可能粒子、及び前記抗原がコーティングされていない第2の検出可能粒子と混合し、培養して、前記血液試料と前記第1及び第2の粒子の培養された混合物を生成する工程と;
膜厚方向の厚さが3〜25μmであるサンプルチャンバを用意する工程と;
前記チャンバに前記血液試料混合物を充填する工程と;
前記チャンバを光学的にスキャン又は撮像して、個々の白血球により貪食された前記第1及び第2の粒子の平均量を検出し記録する工程と;
前記スキャン又は撮像工程が、前記血液試料のドナーが前記所定の病原体に感染しているとの兆候があることを示すか否かを決定する工程と
を含む方法。 - 前記分析の有効性を決定する工程をさらに含む請求項1に記載の方法。
- 前記所定の病原体が、ライム病の存在を示すボレリア(Borrelia)菌である請求項1に記載の方法。
- 前記チャンバの膜厚方向の厚さが約4〜5μmである請求項1に記載の方法。
- 抗凝固処理全生血の単層薄膜試料を分析して、血液ドナーの所定の病原体による最近の曝露又は現在の感染の有無を調べる方法であって、標的検体が、特定の単数又は複数の抗原がコーティングされた粒子に対する、血液試料に含まれる白血球による特定の貪食作用及び/又は結合作用の有無である方法において、
前記血液試料を、前記標的検体に特異的な抗原がコーティングされた第1の検出可能粒子と混合し、培養して、前記血液試料と前記第1の粒子の培養された混合物を生成する工程と;
膜厚方向の厚さが3〜12μmであるサンプルチャンバを用意する工程と
前記チャンバに前記血液試料混合物を充填する工程と;
前記チャンバを光学的にスキャン又は撮像して、個々の白血球により貪食された前記第1の粒子の平均量を検出し記録する工程と;
前記スキャン又は撮像工程が、前記血液試料のドナーが前記所定の病原体に感染しているとの兆候があることを示すか否かを決定する工程と
を含む方法。 - 前記培養された混合物に、前記抗原がコーティングされていない第2の独立して検出可能な粒子を含有させて、前記血液試料と前記第1及び第2の粒子の培養された混合物を生成する工程をさらに含む請求項5に記載の方法。
- 前記分析の有効性を決定する工程をさらに含む請求項6に記載の方法。
- 前記所定の病原体が、ライム病の存在を示すボレリア(Borrelia)菌である請求項5に記載の方法。
- 前記チャンバの膜厚方向の厚さが約6μmである請求項5に記載の方法。
- 抗凝固処理全生血試料の単層薄膜試料を分析して、病原体による最近の曝露又は現在の感染の有無を調べる方法であって、
膜厚方向の厚さが約3〜25μmであるサンプルチャンバ、並びに、標的検体に特異的な少なくとも1つの特定の抗原がコーティングされた1つ以上の第1の粒子、及び前記抗原がコーティングされていない1つ以上の第2の粒子とを用意する工程と;
前記チャンバ内に血液試料混合物を配置し、前記血液試料混合物を培養して、血液試料と前記第1及び第2の粒子の培養された混合物を生成する工程と;
前記チャンバを光学的にスキャン又は撮像して、個々の白血球により貪食された前記第1及び第2の粒子の平均量を検出し記録し、前記チャンバ内の試料及び粒子の画像を作成する工程と;
前記チャンバ内の前記試料及び粒子の画像を使用して、前記血液試料が前記所定の病原体に最近感染していた又は現在感染しているとの兆候があるか否かを決定する工程と
を含む方法。 - 前記分析の有効性を決定する工程をさらに含む請求項10に記載の方法。
- 前記チャンバの膜厚方向の厚さが約6〜12μmである請求項11に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/417,296 US9395365B2 (en) | 2009-04-02 | 2009-04-02 | Detection of infectious disease in a human or animal by measuring specific phagocytosis in a thin film sample of their anticoagulated blood |
US12/417,296 | 2009-04-02 | ||
PCT/US2010/029662 WO2010115024A1 (en) | 2009-04-02 | 2010-04-01 | Detection of infectious disease in a human or animal by measuring specific phagocytosis in a thin film sample of their anticoagulated blood |
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JP2012522992A true JP2012522992A (ja) | 2012-09-27 |
JP5670423B2 JP5670423B2 (ja) | 2015-02-18 |
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US (1) | US9395365B2 (ja) |
EP (1) | EP2414537B1 (ja) |
JP (1) | JP5670423B2 (ja) |
CN (1) | CN102388146B (ja) |
AU (1) | AU2010232583B2 (ja) |
CA (1) | CA2757245C (ja) |
WO (1) | WO2010115024A1 (ja) |
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WO2017048881A1 (en) | 2015-09-14 | 2017-03-23 | Essenlix Corporation | Device and system for collecting and analyzing vapor condensate, particularly exhaled breath condensate, as well as method of using the same |
WO2018119318A1 (en) | 2016-12-21 | 2018-06-28 | Essenlix Corporation | Devices and methods for authenticating a sample and use of the same |
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WO2018148469A1 (en) | 2017-02-08 | 2018-08-16 | Essenlix Corp. | Bio/chemical material extraction and assay |
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JP7004732B2 (ja) | 2017-02-09 | 2022-01-21 | エッセンリックス コーポレーション | 異なる間隔の高さを使用するアッセイ法 |
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JP2001517921A (ja) * | 1995-06-07 | 2001-10-09 | ユニヴァーシティー オブ コネティカット | 早期診断検査 |
JP2007532881A (ja) * | 2004-04-07 | 2007-11-15 | ウォードロウ パートナーズ エルピー | 生体液を分析する使い捨てチャンバ |
WO2008151088A2 (en) * | 2007-06-01 | 2008-12-11 | University Of Maryland, Baltimore | Immunoglobulin constant region fc receptor binding agents |
JP2010529043A (ja) * | 2007-06-01 | 2010-08-26 | ユニバーシティー オブ メリーランド,ボルティモア | 免疫グロブリン定常領域Fc受容体結合因子 |
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AU2010232583B2 (en) | 2013-06-20 |
JP5670423B2 (ja) | 2015-02-18 |
CA2757245C (en) | 2015-05-26 |
EP2414537A1 (en) | 2012-02-08 |
CN102388146B (zh) | 2015-12-16 |
CN102388146A (zh) | 2012-03-21 |
AU2010232583A1 (en) | 2011-10-27 |
EP2414537B1 (en) | 2018-12-19 |
WO2010115024A1 (en) | 2010-10-07 |
CA2757245A1 (en) | 2010-10-07 |
US9395365B2 (en) | 2016-07-19 |
US20100255509A1 (en) | 2010-10-07 |
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