JP2011200260A - Feed for fish of thunnus - Google Patents
Feed for fish of thunnus Download PDFInfo
- Publication number
- JP2011200260A JP2011200260A JP2011158363A JP2011158363A JP2011200260A JP 2011200260 A JP2011200260 A JP 2011200260A JP 2011158363 A JP2011158363 A JP 2011158363A JP 2011158363 A JP2011158363 A JP 2011158363A JP 2011200260 A JP2011200260 A JP 2011200260A
- Authority
- JP
- Japan
- Prior art keywords
- feed
- fish
- tuna
- tuna fish
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
Description
本発明はマグロ属魚類用飼料に関する。その目的は、世界のマグロ属魚類資源が減少に向かうなかで、その人為的資源管理に不可欠な種苗生産を、確実かつ効率的に実施するための人工飼料を提供することにある。 The present invention relates to a tuna fish feed. The aim is to provide an artificial feed to ensure reliable and efficient seed production that is indispensable for man-made resource management as the world's tuna fish resources are decreasing.
本発明の出願人である近畿大学の水産研究所は、クロマグロの種苗生産に世界で初めて成功した研究機関であるとともに、種苗生産を産業的に実施できる技術と規模を併せ持つ希少な施設である。
近年、マグロ属魚類資源の減少は著しく、絶滅危惧種にも指定されようとしている。この主たる原因はマグロ属漁獲量の60%を消費する日本にあるとされ、資源回復に向けた早急な研究、技術開発および情報の発信が望まれている。
The Fisheries Research Institute of Kinki University, the applicant of the present invention, is the world's first successful research institute for bluefin tuna seedling production, and is a rare facility that has both technology and scale to enable industrial seedling production.
In recent years, tuna fish stocks have declined significantly and are being designated as endangered species. The main cause is considered to be in Japan, which consumes 60% of the tuna catch, and it is hoped that research, technology development and information dissemination will be made for resource recovery.
従来、マグロ属魚類の種苗生産において、ふ化3日後からシオミズツボワムシを、10日後からマダイやイシダイの卵やふ化仔魚を給与し、稚魚に変態した3週間後からは徐々に、細断したマイワシ、イカナゴ、マアジ、他の多獲性魚類などの生餌に切り替える飼育方法が採用されてきた。特に、マグロ属魚類の稚魚は他の魚種に比べて成長が著しく速いことから、日出から日没まで連続的に給餌する必要があり、また消化性の低い餌飼料を与えると成長が大きく停滞する。 Conventionally, in the seedling production of tuna fish, fallow beetles were fed from 3 days after hatching, and red sea bream and sea bream eggs and hatched larvae were fed 10 days later, and gradually shredded from 3 weeks after transformation into fry. Breeding methods have been adopted that switch to raw foods such as sardines, squid, horse mackerel, and other high-catch fish. In particular, tuna larvae grow significantly faster than other fish species, so they need to be fed continuously from sunrise to sunset, and growth is greater when given a low-digestible diet. Stagnant.
生餌の給餌において、その調製に際しては細断に長時間を要するために生産効率が低く、加えて、栄養素の過不足、疾病の経口感染、自家汚染などの問題を生じる。もし、マグロ属魚類用の人工飼料による飼育が可能になれば、生餌摂取による伝染性細菌およびウイルス症の経口感染の防止や投薬、ストレス耐性低下の防止などが可能になるばかりか、給餌の省力・機械化に貢献し、安定した種苗生産技術の確立をも可能にする。さらには、養殖用はもちろん資源増強を目指した放流用種苗の生産を、省力的かつ効率的に行なうことができる。 In the preparation of raw food, the preparation takes a long time to chop, so that the production efficiency is low. In addition, problems such as excess and deficiency of nutrients, oral infection of diseases, and self-contamination occur. If artificial feed for tuna fish can be raised, it will be possible to prevent oral infection and administration of infectious bacteria and virosis by ingesting raw food, prevent stress tolerance reduction, etc. Contributes to labor saving and mechanization, and enables establishment of stable seed production technology. Furthermore, it is possible to save and efficiently produce seedlings for release aiming at resource enhancement as well as for aquaculture.
しかしながら、これまでに、マグロ属魚類用飼料のタンパク質源として、酵素処理魚粉の高い利用性が明らかにされただけで(特許文献1参照)、脂質、糖質およびビタミンCの給源と、それらの適正な配合割合については不明であることから、人工飼料は実用の段階に至っていないのが現状である。 However, so far only the high availability of enzyme-treated fish meal has been clarified as a protein source for tuna fish feed (see Patent Document 1), and sources of lipids, carbohydrates and vitamin C, and their Since the appropriate blending ratio is unknown, the artificial feed has not yet reached the stage of practical use.
本発明は、優れたクロマグロの種苗生産や飼育技術をもち、好ましい飼育環境を保持できる飼育施設を用いて、鋭意研究を行うことができる本発明者らにおいてのみ成しえたものである。本発明の課題は、マグロ属魚類の栄養要求を満たすとともに、増重率、増体長率、生残率、飼料効率等の飼育成績が優れるマグロ属魚類用の人工飼料を提供することによって、マグロ属魚類の種苗生産、養殖、天然資源の増強と管理に貢献することを目的とする。 The present invention has been achieved only by the present inventors who can conduct intensive research using breeding facilities that have excellent bluefin tuna seedling production and breeding techniques and can maintain a favorable breeding environment. An object of the present invention is to provide an artificial feed for tuna fish that satisfies the nutritional requirements of tuna fish and has excellent breeding results such as weight gain, weight gain rate, survival rate, feed efficiency, etc. It aims to contribute to seedling production, aquaculture, and enhancement and management of natural resources.
請求項1に係る発明は、タンパク源として有機溶媒により脱脂された脱脂酵素処理魚粉、脂質源として極性脂質を含む動物油脂又は植物油脂、及び糖質源としてα化澱粉を含有してなるとともに、ビタミンCを200mg/kg以上配合してなるマグロ属魚類用飼料に関する。
請求項2に係る発明は、前記ビタミンCの配合量が400mg/kg以下であることを特徴とする請求項1に記載のマグロ属魚類用飼料に関する。
請求項3に係る発明は、前記脱脂酵素処理魚粉の含有量が56〜74%であることを特徴とする請求項1又は2に記載のマグロ属魚類用飼料に関する。
請求項4に係る発明は、前記脱脂酵素処理魚粉中の脂質含有量が9%未満であることを特徴とする請求項1乃至3いずれかに記載のマグロ属魚類用飼料に関する。
請求項5に係る発明は、前記動物油脂又は植物油脂の含有量が4〜16%であることを特徴とする請求項1乃至4いずれかに記載のマグロ属魚類用飼料に関する。
The invention according to claim 1 comprises defatted enzyme-treated fish meal degreased with an organic solvent as a protein source, animal oil or vegetable oil containing polar lipids as a lipid source, and pregelatinized starch as a carbohydrate source, The present invention relates to a tuna fish feed comprising 200 mg / kg or more of vitamin C.
The invention according to claim 2 relates to a tuna fish feed according to claim 1, wherein the amount of vitamin C is 400 mg / kg or less.
The invention according to claim 3 relates to a tuna fish feed according to claim 1 or 2, wherein the content of the defatted enzyme-treated fish meal is 56 to 74%.
The invention according to claim 4 relates to a tuna fish feed according to any one of claims 1 to 3, wherein the fat content in the defatted enzyme-treated fish meal is less than 9%.
The invention according to claim 5 relates to a tuna fish feed according to any one of claims 1 to 4, wherein the content of the animal oil or vegetable oil is 4 to 16%.
請求項6に係る発明は、前記α化澱粉の含有量が10%以下であることを特徴とする請求項1乃至5いずれかに記載のマグロ属魚類用飼料に関する。
請求項7に係る発明は、前記マグロ属魚類用飼料が、マグロ属魚類の稚魚用飼料であることを特徴とする請求項1乃至6いずれかに記載のマグロ属魚類用飼料に関する。
請求項8に係る発明は、前記マグロ属魚類の稚魚が、体重が0.2〜50gの稚魚であることを特徴とする請求項7に記載のマグロ属魚類用飼料に関する。
The invention according to claim 6 relates to a tuna fish feed according to any one of claims 1 to 5, wherein the pregelatinized starch content is 10% or less.
The invention according to claim 7 relates to a tuna fish feed according to any one of claims 1 to 6, wherein the tuna fish feed is a tuna fish fry feed.
The invention according to claim 8 relates to the tuna fish feed according to claim 7, wherein the fry of the tuna fish is a fry having a weight of 0.2 to 50 g.
本発明のマグロ属魚類用飼料は、人工飼料である。したがって、従来の生餌が抱えていた問題点、即ち、給餌に伴う生産効率の低下、疾病の経口感染、水槽や養殖場の水質の悪化等の自家汚染などを防ぐことができる。
本発明のマグロ属魚類用飼料は、これのみで長時間飼育した場合でも、マグロ属魚類の栄養要求を完全に満たすことから、増重率、増体長率、生残率、飼料効率等の飼育成績が優れるだけでなく、マグロ属魚類のビタミン欠乏による免疫能低下を防ぐ。
本発明のマグロ属魚類用飼料は、マグロ属魚類の稚魚に与えられた場合、特に優れた飼育成績をもたらす。
The tuna fish feed of the present invention is an artificial feed. Therefore, it is possible to prevent problems with conventional live foods, that is, self-contamination such as a decrease in production efficiency associated with feeding, oral infection of diseases, and deterioration of water quality in aquariums and farms.
Even if the feed for tuna fish of the present invention alone is bred for a long time, it completely satisfies the nutritional requirements of the fish of the genus Tuna, so the breeding rate, weight gain rate, survival rate, feed efficiency, etc. Not only does it perform well, it also prevents immune loss due to vitamin deficiencies in tuna fish.
The feed for tuna fish of the present invention provides particularly excellent rearing results when fed to juvenile tuna fish.
マグロ属魚類の成長、特にその稚魚期の成長は、ブリやマダイのそれらの2〜3倍であり、それらの成長を支えるための餌飼料は、摂餌活性が高く、必要量の各栄養素を含み、且つ、それらが消化しやすいものでなければならない。また、特に稚魚の時期は、噛み合いや水槽壁への衝突死が頻発するなど、種苗生産の成否を決定する重要な期間でもある。
本発明のマグロ属魚類用飼料は摂餌活性が高く、必要とされる栄養素含量を含み、消化しやすい。本発明の他の利点は、その保管のための冷凍庫が不必要で、手撒が容易になるとともに自動給餌機を用いることができることから、給餌に掛かる時間や労力を大きく節約できる。
従って、マグロ属魚類の種苗生産、養殖、天然資源の増強と管理に貢献する。
The growth of tuna fish, especially in the juvenile stage, is 2 to 3 times that of yellowtail and red sea bream, and the feed to support their growth has high feeding activity and the necessary amount of each nutrient. It must contain and be easy to digest. In particular, the fry stage is also an important period for determining success or failure of seedling production, such as frequent occurrence of biting and collision death on the aquarium wall.
The tuna fish feed of the present invention has high feeding activity, contains the required nutrient content, and is easy to digest. Another advantage of the present invention is that a freezer is not required for storage, the handling is facilitated, and an automatic feeding machine can be used, so that time and labor required for feeding can be greatly saved.
Therefore, it contributes to seed production, aquaculture, and natural resource enhancement and management of Tuna fish.
本発明のマグロ属魚類用飼料について説明する。
本発明に係るマグロ属魚類には、クロマグロ(Thunnus orientalis)、大西洋クロマグロ(Thunnus thynnus)、キハダ(Thunnus albacares)、メバチ(Thunnus obesus)、ビンナガ(Thunnus alalunga)、ミナミマグロ(Thunnus maccoyii)、コシナガ(Thunnus tonggol)が含まれる。
The tuna fish feed of the present invention will be described.
Tuna genus fishes according to the present invention, bluefin tuna (Thunnus orientalis), Atlantic bluefin (Thunnus thynnus), KIHA Da (Thunnus albacares), Meba Ji (Thunnus obesus), Bin'na moth (Thunnus alalunga), SBT (Thunnus maccoyii), Cosina moth (Thunnus tonggol) are included.
本発明のマグロ属魚類用飼料は、タンパク源として脱脂酵素処理魚粉、脂質源として極性脂質を含む動物油脂又は植物油脂を、及び糖質源としてα化澱粉を含有してなり、さらに、ビタミンCが多量に配合されてなる。
前記ビタミンCは、マグロ属魚類用飼料1kgあたり、200mg以上配合されてなり、好ましくは200〜400mg配合されてなる。この理由は、ビタミンCが200mg以上配合されてなるマグロ属魚類用飼料は、成長や脳・肝臓のビタミンC含量を正常に維持するため好ましく、一方、200mg未満の場合は、生残率や飼育成績は著しく劣る。
The feed for tuna fish of the present invention comprises defatted enzyme-treated fish meal as a protein source, animal oil or vegetable oil containing polar lipids as a lipid source, and pregelatinized starch as a sugar source. Is blended in a large amount.
The vitamin C is formulated in an amount of 200 mg or more per 1 kg of tuna fish feed, preferably 200 to 400 mg. The reason for this is that tuna fish feed containing 200 mg or more of vitamin C is preferable for maintaining normal growth and vitamin C content of the brain / liver. On the other hand, if it is less than 200 mg, survival rate and breeding The results are significantly inferior.
前記ビタミンCとは、L‐アスコルビン酸、L‐アスコルビン酸カルシウム、L‐アスコルビン酸‐2‐燐酸マグネシウム(APM)、L‐アスコルビン酸‐2‐リン酸ナトリウム・カリウム等のアスコルビン酸誘導体、これらのビタミンC類の2以上を任意の割合で配合した混合物、あるいはビタミンC含有天然物をマグロ属魚類用飼料に添加することにより配合される。 Examples of vitamin C include ascorbic acid derivatives such as L-ascorbic acid, calcium L-ascorbate, L-ascorbic acid-2-magnesium phosphate (APM), L-ascorbic acid-2-sodium phosphate and potassium, etc. It mixes | blends by adding the mixture which mix | blended two or more of vitamin C in arbitrary ratios, or the vitamin C containing natural product to the feed for fish of the genus Tuna.
タンパク源として脱脂酵素処理魚粉を含有する。マグロ属魚類用飼料中の脱脂酵素処理魚粉の含有量は、好ましくは56〜74%である。
本発明で使用する脱脂酵素処理魚粉は、好ましくは特許文献1に記載の酵素処理魚粉を脱脂したものを使用する。即ち、本発明に係る脱脂酵素処理魚粉は以下の特徴を有する酵素処理魚粉を脱脂処理したものである。
Contains defatted enzyme-treated fish meal as a protein source. The content of the defatted enzyme-treated fish meal in the tuna fish feed is preferably 56 to 74%.
The defatted enzyme-treated fish meal used in the present invention is preferably a defatted enzyme-treated fish meal described in Patent Document 1. That is, the defatted enzyme-treated fish meal according to the present invention is obtained by defatting an enzyme-treated fish meal having the following characteristics.
まず酵素処理魚粉を製造するにあたり、魚粉が酵素処理される。使用される酵素は特に限定されないが、好ましくは、ペプチダーゼ、プロテアーゼ又はプロテイナーゼの生物由来酵素が用いられる。酵素処理魚粉は、好ましくはニシン目ニシン科(Clupeidae)、ニシン目カタクチイワシ科(Engraulidae)、スズキ目アジ科(Carangidae)又はスズキ目サバ科(Scombridae)から選択される1種以上の魚粉から製造される。
例えば、マイワシ(Sardinops melanostictus)やカタクチイワシ(Engraulis japonicus)、アンチョビー(ニシン目カタクチイワシ科(Engraulidae)に属する)などのイワシ類、マサバ(Scomber japonicus)やゴマサバ(Scomber australasicus)などのサバ類、マアジ(Trachurus japonicus)、マルアジ(Decapterus maruadsi)のアジ類又は大西洋ニシン(Clupea harengus)などニシン類が挙げられる。また、上記魚類の漁獲海域は特に限定されないが、ペルー・チリ付近の太平洋東部沿岸で漁獲されたものが多く用いられる。このうちアジ類を含有する魚粉からなる酵素処理魚粉は、アミノ酸バランスや保存性に優れているため、特に好ましく用いられる。
First, in producing enzyme-treated fish meal, the fish meal is enzymatically treated. The enzyme to be used is not particularly limited, but a peptidase, protease or proteinase biological enzyme is preferably used. The enzyme-treated fish meal is preferably produced from one or more fish meals selected from the order of the Coleidae, Clapidae, Engraulidae, Carangidae, or Scombridae The
For example, sardines such as sardine (Sardinops melanosticus), anchovy (Engraulis japonicus), anchovy (belonging to Herringidaceae (Engraulidae)), sardine ass (Somabera scorpus) herbs such as japonicus, decapterus maradsi, or Atlantic herring. Moreover, although the fish sea area of the said fish is not specifically limited, what was caught on the eastern Pacific coast near Peru and Chile is often used. Among these, enzyme-treated fish meal comprising fish meal containing horse mackerel is particularly preferably used because of its excellent amino acid balance and storage stability.
前記脱脂酵素処理魚粉中に含まれるその他の成分として、ペプチド、ポリペプチド、アミノ酸、ビタミン類、ミネラル、脂肪酸が含まれている。
前記アミノ酸としては、リジン、メチオニン、トリプトファン、ヒスチジン、アルギニン等の必須アミノ酸等が含まれる。前記ビタミン類としては、塩化コリン、ビタミンA、ビタミンD、ビタミンB群等が含まれ、前記ミネラルとしては、リン、カルシウム、セレン、亜鉛等が含まれている。
As other components contained in the defatted enzyme-treated fish meal, peptides, polypeptides, amino acids, vitamins, minerals, and fatty acids are included.
The amino acid includes essential amino acids such as lysine, methionine, tryptophan, histidine, arginine and the like. The vitamins include choline chloride, vitamin A, vitamin D, vitamin B group and the like, and the minerals include phosphorus, calcium, selenium, zinc and the like.
本発明に係る脱脂酵素処理魚粉とは、前記酵素処理魚粉に脱脂処理を施したものである。この脱脂処理とは、食品安全法で認められている有機溶媒,例えばn-ヘキサンなどで抽出することにより行われる。
本発明に係る脱脂酵素処理魚粉は、好ましくは、脱脂酵素処理魚粉中の脂質含有量が9%未満である。この理由は、脂質含量が9%以上の場合、極性脂質を含む動物油脂又は植物油脂を必要量配合すると、飼料の脂質含量がマグロ属魚類の要求量を越えるため好ましくないからである。脱脂酵素処理魚粉の脂肪酸組成を表1に示す。
The defatted enzyme-treated fish meal according to the present invention is obtained by subjecting the enzyme-treated fish meal to a defatted treatment. This degreasing treatment is performed by extraction with an organic solvent approved by the Food Safety Law, such as n-hexane.
The defatted enzyme-treated fish meal according to the present invention preferably has a lipid content of less than 9% in the defatted enzyme-treated fish meal. This is because, when the lipid content is 9% or more, blending the necessary amount of animal fat or vegetable fat containing polar lipids is not preferable because the lipid content of the feed exceeds the required amount of tuna fish. Table 1 shows the fatty acid composition of the defatted enzyme-treated fish meal.
さらに詳細には、本発明に係る脱脂酵素処理魚粉中、蛋白は65〜80%、好ましくは70〜80%含有されている。 More specifically, the protein is contained in the defatted enzyme-treated fish meal according to the present invention in an amount of 65 to 80%, preferably 70 to 80%.
脂質源として極性脂質を含む動物油脂又は植物油脂を使用し、この油脂は好ましくは極性脂質を多量に含む。動物油脂又は植物油脂中に極性脂質は、好ましくは20%以上含まれる。前記油脂としては、サケ・マス類の卵巣(スジコ)油が好適に使用できるが、さらに好ましくは、その極性画分にドコサヘキサエン酸(DHA)を多量に含む動物性油脂が使用され、前記DHAを好ましくは20%以上含むスジコ油が好適である。尚、このスジコ油は魚卵から抽出した油脂である。 Animal fats or vegetable oils and fats containing polar lipids are used as the lipid source, and these fats and oils preferably contain a large amount of polar lipids. The polar lipid is preferably contained in animal oil or vegetable oil or vegetable oil by 20% or more. As the fats and oils, ovary (sujiko) oil of salmon and trout can be suitably used. More preferably, animal fats and oils containing a large amount of docosahexaenoic acid (DHA) are used in the polar fraction, Sujiko oil containing 20% or more is preferable. In addition, this sujiko oil is the fats and oils extracted from the fish egg.
本発明に係る極性油脂は、好ましくはその脂質含量が90%以上、より好ましくは95%以上である。本発明で使用可能なスジコ油の脂肪酸組成の一例を表2に示す。 The polar fat according to the present invention preferably has a lipid content of 90% or more, more preferably 95% or more. Table 2 shows an example of the fatty acid composition of sujiko oil usable in the present invention.
本発明のマグロ属魚類用飼料中の前記極性脂質を含む動物油脂又は植物油脂の含有量は、好ましくは4〜16%、より望ましくは8〜12%である。但し、飼料中に含まれるその他の成分由来の脂質の総含有量が好ましくは、10〜22%、より望ましくは15〜18%となるように調整される。飼料中の脂質の総含有量が、10%未満或いは22%を超えると、マグロ属魚類の生残率や成長、飼育成績が低下するため、いずれの場合も好ましくない。 The content of the animal oil or vegetable oil or fat containing the polar lipid in the tuna fish feed of the present invention is preferably 4 to 16%, more preferably 8 to 12%. However, the total content of lipids derived from other components contained in the feed is preferably adjusted to 10 to 22%, more desirably 15 to 18%. If the total content of lipids in the feed is less than 10% or more than 22%, the survival rate, growth, and breeding performance of tuna fish will be reduced, which is not preferable in any case.
糖質源としては、α化澱粉が使用される。前記α澱粉としては、馬鈴薯、キャッサバ、トウモロコシ等の植物性のα化澱粉が挙げられる。本発明のマグロ属魚類用飼料中のα化澱粉の含有量は,好ましくは10%以下、より望ましくは3〜8%である。
従来使用されている他の魚類用の飼料と比較して、マグロ属魚類用飼料中のα化澱粉の含有量は少ない。即ち、一般的な草食又は雑食魚類用の飼料中の糖質成分の配合量は40〜20%であり、マグロ属魚類以外の肉食魚用飼料には、11〜20%程度含有される。このような含有量で、即ち、α化澱粉が10%を超えると、マグロ属魚類の成長や飼育成績が低下するため好ましくない。
As the sugar source, pregelatinized starch is used. Examples of the α starch include plant α-starch such as potato, cassava, and corn. The content of pregelatinized starch in the tuna fish feed of the present invention is preferably 10% or less, more desirably 3 to 8%.
Compared to other conventionally used fish feeds, the content of pregelatinized starch in the tuna fish feed is low. That is, the compounding quantity of the saccharide | sugar component in the feed for general herbivores or omnivorous fish is 40 to 20%, and about 11 to 20% is contained in the feed for carnivorous fish other than the tuna fish. With such a content, that is, when the pregelatinized starch exceeds 10%, it is not preferable because the growth and breeding performance of the tuna fish is lowered.
本発明のマグロ属魚類用飼料のエネルギー含量は、好ましくは5190〜5300kcal/kgである。この範囲のエネルギー含量を有するマグロ属魚類用飼料は、成長や飼育成績に優れる。 The energy content of the tuna fish feed of the present invention is preferably 5190-5300 kcal / kg. A tuna fish feed having an energy content in this range is excellent in growth and breeding performance.
本発明のマグロ属魚類用飼料には、任意に薬剤、免疫賦活剤、ハーブ、栄養剤等の添加剤を含んでもよい。本発明のマグロ属魚類用飼料は水分を含んだモイストペレット、乾燥したドライペレットあるいはエクストルーダーなどで調製したEP・EXペレットのいずれの形態でも使用できる。 The feed for tuna fish of the present invention may optionally contain additives such as drugs, immunostimulants, herbs and nutrients. The tuna fish feed of the present invention can be used in any form of moisture-containing moist pellets, dried dry pellets or EP / EX pellets prepared with an extruder.
本発明のマグロ属魚類用飼料は、マグロ属魚類の成魚又は稚魚のいずれに対しても使用できるが、好ましくはマグロ属魚類の稚魚に対して使用される。 The feed for tuna fish of the present invention can be used for either adult fish or fry of tuna fish, but is preferably used for fry of tuna fish .
以下、実施例に基づいて本発明のマグロ属魚類用飼料を詳細に説明するが、本発明はこれに限定されるものではない。
<試験例1>マグロ属魚類用飼料のビタミンC配合量に関する試験
マグロ属魚類のビタミンC要求量はこれまで明らかにされていない。そこで、ビタミンCの給源としてアスコルビン酸‐2‐燐酸マグネシウム(APM)を異なる割合で飼料に配合し、肝臓および脳におけるビタミンC含量から、その要求性と適正配合量を明らかにした。
Hereinafter, although the tuna fish feed of the present invention will be described in detail based on examples, the present invention is not limited thereto.
<Test Example 1> Test on Vitamin C Content of Tuna Fish Feed Vitamin C requirements for tuna fish have not been clarified so far. Therefore, ascorbic acid-2-magnesium phosphate (APM) was blended into the feed as a source of vitamin C, and the requirement and proper blending amount were clarified from the vitamin C content in the liver and brain.
(1)供試魚および飼育方法
近畿大学水産養殖種苗センター浦神事業場で、人工種苗生産した平均体重3.05gのクロマグロ稚魚を、15トンコンクリート製八角水槽に60尾ずつ収容して8試験区を設けた。各水槽には30 L/minで濾過海水を注水した。期間中の平均水温は25.1℃、平均溶存酸素量は8.45mg/Lであった。
表3に示した試験飼料組成に従い、試験飼料を作成した。原料を良く混合してから外割で30%の水道水を加え、直径1.4〜1.8mmのペレットに成型し、凍結乾燥機でドライペレットに調製した。飼育期間は14日間とし毎日7:00、9:00、11:00、13:00、15:00および17:00に、各試験飼料を所定の試験区に飽食給与した。なお、試験区は各試験飼料につき2反復区を設けた。
(1) Test Fish and Breeding Methods At the Kinki University Aquaculture Seedling Center Urakami Plant, artificial fishes and seedlings produced with an average weight of 3.05 g of bluefin tuna larvae were housed in a 15-ton concrete octagonal aquarium with 8 fish in 8 test zones. Was provided. Filtered seawater was poured into each water tank at 30 L / min. The average water temperature during the period was 25.1 ° C., and the average dissolved oxygen amount was 8.45 mg / L.
A test feed was prepared according to the test feed composition shown in Table 3. After mixing the raw materials well, 30% tap water was added in an external ratio, formed into pellets with a diameter of 1.4 to 1.8 mm, and prepared into dry pellets with a freeze dryer. The breeding period was 14 days, and each test feed was satiety fed to a predetermined test section at 7:00, 9:00, 11:00, 13:00, 15:00, and 17:00 every day. In addition, the test group provided 2 repetition groups for each test feed.
試験飼料にはタンパク質源として酵素処理魚粉(BIO-CP/ナガセ生化学品販売(株))を、脂質源としてスケトウダラ肝油9重量部に対してDHA油(70%ドコサヘキサエン酸:播磨化成(株)製)1重量部を混合したものを、糖質源としてα化デンプンを、ビタミンおよびミネラル混合物としてHalver処方のものを、ビタミンC源としてアスコルビン酸‐2‐燐酸マグネシウム(APM:昭和電工(株)製)をそれぞれ用いた。また、摂餌促進物質としてグルタミン酸8.5:ヒスチジン232.8:IMP(イノシン酸2Na塩)200.9(重量比)からなる混合物を添加した。尚、前記酵素処理魚粉と摂餌促進物質については特許文献1に記載されているものを使用する。尚、表3中APM‐0、APM‐500、APM‐1000、APM‐2000(0、500、1000、2000mg/kg)とは、ビタミンCが飼料1kg中にそれぞれ、0、200、400、800mg配合されていることを示す。 In the test feed, enzyme-treated fish meal (BIO-CP / Nagase Biochemical Sales Co., Ltd.) is used as a protein source, and DHA oil (70% docosahexaenoic acid: Harima Kasei Co., Ltd.) is used as a lipid source against 9 parts by weight of walleye pollack liver oil. 1) parts by weight of the mixture, pregelatinized starch as the sugar source, Halver prescription as the vitamin and mineral mixture, ascorbic acid-2-magnesium phosphate as the vitamin C source (APM: Showa Denko K.K.) Used). Moreover, the mixture which consists of glutamic acid 8.5: histidine 232.8: IMP (inosinic acid 2Na salt) 200.9 (weight ratio) was added as a feeding promoting substance. In addition, what is described in patent document 1 is used about the said enzyme-treated fish meal and a feeding promoting substance. In Table 3, APM-0, APM-500, APM-1000, and APM-2000 (0, 500, 1000, 2000 mg / kg) are 0, 200, 400, and 800 mg of vitamin C in 1 kg of feed, respectively. Indicates that it is blended.
APM‐0、APM‐500、APM‐1000、APM‐2000区それぞれについて、肝臓及び脳のビタミンC含量を測定した。測定は、HPLCを用いる定法により行った。
尚、表3中には試験飼料の一般成分として、粗タンパク質、粗脂質、粗灰分、糖質等と、APM含量を示した。
The liver and brain vitamin C contents were measured for APM-0, APM-500, APM-1000, and APM-2000, respectively. The measurement was performed by a conventional method using HPLC.
In Table 3, as general components of the test feed, crude protein, crude lipid, crude ash, carbohydrate, etc., and APM content are shown.
(2)飼育成績
14日間の飼育成績を表4に示す。期間中にはいずれの試験区でも衝突死が頻繁に起こり、終了時における生残率は20%と顕著に低くなった。しかし、1日1尾当たりの摂餌量、成長率などはAPM‐0区が、他のAPM‐500、APM‐1000およびAPM‐2000区より低かった。
(2) Rearing results Table 4 shows the rearing results for 14 days. During the period, collision deaths frequently occurred in any of the test plots, and the survival rate at the end was remarkably low at 20%. However, food intake per day, growth rate, etc. were lower in the APM-0 plot than in the other APM-500, APM-1000 and APM-2000 plots.
APM‐0区では試験開始7日後より、遊泳・摂餌不活発、痩身、群の形成不全などのビタミンC欠乏症と考えられる症状がみられた。 In APM-0, 7 days after the start of the test, symptoms considered to be vitamin C deficiency such as inactivity of swimming / feeding, slimming, and dysplasia of the group were observed.
肝臓および脳のビタミンC含量を図1に示す。いずれの臓器における含量もAPM‐1000区からAPM‐2000区にかけて高くほぼ一定の値を維持した。 The vitamin C content of the liver and brain is shown in FIG. The content in all organs was high and maintained almost constant from APM-1000 to APM-2000.
表4で示す飼育成績および図1で示す臓器のビタミンC含量から、マグロ属魚類用飼料中の適正なビタミンC含量は、APMにして500mg/kg飼料前後、ビタミンCとして200mg/kg、好ましくは、APMにして1000mg/kg飼料以上、ビタミンCとして400mg/kg含まれる飼料が優れていることが分かった。なお、飼育試験ではAPM‐500区で終了時の体重が他の区より若干優れていたが、他の項目に大きな差異や一定の傾向がみられなかった。これは,主に飼育期間が短かったためと考えられる。 From the breeding results shown in Table 4 and the vitamin C content of the organs shown in FIG. 1, the appropriate vitamin C content in the tuna fish feed is around 500 mg / kg feed as APM, 200 mg / kg as vitamin C, preferably It was found that a feed containing 1000 mg / kg or more of APM and 400 mg / kg of vitamin C was excellent. In the breeding test, the weight at the end of the APM-500 group was slightly better than that of the other groups, but there was no significant difference or a certain tendency in other items. This is probably due to the short breeding period.
<試験例2>マグロ属魚類用飼料の適正なタンパク質および脂質含量に関する試験
本発明のマグロ属魚類用飼料のタンパク質源として、n‐ヘキサンで脱脂した酵素処理魚粉(BIO-CP/ナガセ生化学販売(株))と、脂質源としてサケ・マス類の卵巣から調製したスジコ油(日清マリンテック(株))を用いた。これら両者の配合量を補足的に5段階に変化させて、タンパク質および脂質の適正な配合割合について検討した。なお、比較・対照としてイカナゴ切餌を与えた。
<Test Example 2> Test on appropriate protein and lipid content of tuna fish feed As a protein source of tuna fish feed of the present invention, enzyme-treated fish meal degreased with n-hexane (BIO-CP / Nagase Biochemical Sales) Co., Ltd.) and sudiko oil (Nisshin Marine Tech Co., Ltd.) prepared from salmon and trout ovaries as a lipid source. The blending amounts of both of these were supplementarily changed in five stages, and the proper blending ratio of protein and lipid was examined. As a comparison / control, cut squid was given.
(1)供試魚および飼育方法
近畿大学水産養殖種苗センター浦神事業場で人工種苗生産した平均体重0.26gのクロマグロ稚魚を、15トンコンクリート製八角水槽に200尾ずつ収容して各試験区を設けた。各水槽には30 L/minで濾過海水を注水した。期間中の水温は26.6〜27.1℃、溶存酸素料は7.54〜8.09mg/Lであった。
表5に示した試験飼料組成に従い、試験飼料を作成した。原料を良く混合してから外割で15%の水道水を加え、試験用造粒機で直径0.75〜1.2mmのペレットに調製した。飼育期間は9日間とし毎日5:30、8:00、11:00、13:00、15:00および16:30に、所定の飼料を各試験区に飽食給与した。なお、試験区はそれぞれ2反復区を設けた。
尚、表5には試験飼料の一般成分として、粗タンパク質、粗脂質、粗灰分、糖質などと、爆発熱量計で測定したエネルギー含量を示した。
(1) Test fish and rearing method 200 tuna fry fish with an average weight of 0.26g produced by artificial seedling production at the Urakami Plant of Kinki University Aquaculture Seedling Center are housed 200 units each in a 15-ton concrete octagonal aquarium. Provided. Filtered seawater was poured into each water tank at 30 L / min. The water temperature during the period was 26.6 to 27.1 ° C., and the dissolved oxygen was 7.54 to 8.09 mg / L.
According to the test feed composition shown in Table 5, a test feed was prepared. After mixing the raw materials well, 15% of tap water was added in an external ratio and prepared into pellets having a diameter of 0.75 to 1.2 mm with a test granulator. The breeding period was 9 days, and a predetermined feed was satisfactorily fed to each test section at 5:30, 8:00, 11:00, 13:00, 15:00 and 16:30 every day. In addition, each test section was provided with two repeated sections.
Table 5 shows, as general components of the test feed, crude protein, crude lipid, crude ash, carbohydrates, and the like, and the energy content measured with an explosion calorimeter.
(2)飼育成績
9日間の飼育成績を表6に示した。終了時における平均体重、平均体長、平均増重率、飼料効率などは、脱脂酵素処理魚粉68%とスジコ油8%を配合した飼料3区が最も優れており、イカナゴ区に比べても全く遜色はなかった。例えば、飼料効率についてみると、3区が120%、次いで4区118%、5区113%、2区112%、1区が94.7%、そしてイカナゴ区が69.2%と最も低かった。
(2) Rearing results Table 6 shows the rearing results for 9 days. The average weight, average body length, average weight gain, feed efficiency, etc. at the end are the best in the 3rd feed with 68% defatted enzyme-treated fish meal and 8% sugico oil. There was no. For example, in terms of feed efficiency, the 3rd ward was the lowest at 120%, followed by the 4th ward 118%, the 5th ward 113%, the 2nd ward 112%, the 1st ward 94.7%, and the Ikanago ward 69.2%. .
一方、表中の生残率をみると、飼料5区が最も高く飼料1区が最も低かったが、それらは61〜69%の範囲で区間差は僅かであった。なお、これらの多くは噛み合いおよび衝突死が原因であった。 On the other hand, looking at the survival rate in the table, the feed 5 ward was the highest and the feed 1 ward was the lowest, but they were in the range of 61-69%, and the difference in the sections was slight. Many of these were caused by biting and collision death.
飼育成績を総合評価すると、飼料2〜4区で優れていたことから、脱脂酵素処理魚粉の適正な配合割合は62〜74%(飼料粗タンパク質含量約55〜65%)、極性脂質を多く含むスジコ油の適正な配合割合は4〜12%(飼料粗脂質含量約14〜22%)であることが分かった。更に詳細には、飼料3及び4区が特に優れていることを考慮すると、脱脂酵素処理魚粉の適正な配合割合は62〜68%(飼料粗タンパク質含量約55〜61%)、極性脂質を多く含むスジコ油の適正な配合割合は8〜12%(飼料粗脂質含量約17〜22%)であることが分かった。また,適正な飼料タンパク質/脂質比および飼料エネルギー/タンパク質比は,それぞれ2.6〜3.5および35.5〜39.4であった。 The overall evaluation of the breeding results showed that it was excellent in feeds 2 to 4; therefore, the proper blending ratio of defatted enzyme-treated fish meal was 62 to 74% (feed crude protein content of about 55 to 65%), and contained a large amount of polar lipids. It was found that the proper blending ratio of sudiko oil was 4 to 12% (feed crude lipid content of about 14 to 22%). More specifically, considering that the feeds 3 and 4 are particularly excellent, the appropriate blending ratio of the defatted enzyme-treated fish meal is 62 to 68% (feed crude protein content of about 55 to 61%) and a large amount of polar lipids. It was found that the proper blending ratio of the sujiko oil to be included was 8 to 12% (feed crude lipid content of about 17 to 22%). The proper feed protein / lipid ratio and feed energy / protein ratio were 2.6-3.5 and 35.5-39.4, respectively.
<試験例3>マグロ属魚類用飼料の適正な糖質含量に関する試験
本発明に係る糖質源には、α化デンプンすなわちα−馬鈴薯デンプンを用いた。試験例2中の表5に示す飼料3を基本にして、α化デンプンの配合量を4段階に変化させ、最適な配合割合について検討した。なお、飼料のタンパク質/脂質比を3.6〜3.8の範囲内とするように調整した。
<Test Example 3> Test for proper carbohydrate content of tuna fish feed For the sugar source according to the present invention, pregelatinized starch, that is, α-potato starch was used. Based on the feed 3 shown in Table 5 in Test Example 2, the blending amount of pregelatinized starch was changed in four stages, and the optimum blending ratio was examined. The protein / lipid ratio of the feed was adjusted to be in the range of 3.6 to 3.8.
(1)供試魚および飼育方法
近畿大学水産養殖種苗センター浦神事業場で人工種苗生産した平均体重1.65gのクロマグロ稚魚を、15トンコンクリート製八角水槽に150尾ずつ収容して各試験区を設けた。各水槽には30L/minで濾過海水を注水した。期間中の水温は26.3〜26.9℃、溶存酸素量は6.29〜 6.89mg/Lであった。
表7に示した試験飼料組成に従い、試験飼料を作成した。原料を良く混合してから外割で15%の水道水を加え、試験用造粒機で直径3.0mmのペレットに調製した。飼育期間は8日間とし毎日5:30、8:00、11:00、13:00、15:00及び16:30に、所定の飼料を各試験区に飽食給与した。なお、試験区はそれぞれ3反復区を設けた。
尚、表7中には試験飼料の一般成分として、粗タンパク質,粗脂質,粗灰分,糖質などと,爆発熱量計で測定したエネルギー含量を示した。
(1) Test Fish and Breeding Methods Each of the test plots was stored in a 15-ton concrete octagonal tank with 150 bluefin tuna larvae with an average weight of 1.65 g, produced by artificial seedling production at the Kinki University Aquaculture Seedling Center Urakami Plant. Provided. Filtered seawater was poured into each water tank at 30 L / min. The water temperature during the period was 26.3 to 26.9 ° C., and the dissolved oxygen amount was 6.29 to 6.89 mg / L.
A test feed was prepared according to the test feed composition shown in Table 7. After mixing the raw materials well, 15% tap water was added in an external ratio, and prepared with a test granulator into pellets having a diameter of 3.0 mm. The breeding period was 8 days, and a predetermined feed was satisfactorily fed to each test section at 5:30, 8:00, 11:00, 13:00, 15:00 and 16:30 every day. In addition, each test group provided 3 repetition groups.
In Table 7, as general components of the test feed, crude protein, crude lipid, crude ash, carbohydrates, and the like, and the energy content measured with an explosion calorimeter are shown.
(2)飼育成績
8日間の飼育成績を表8に示した。終了時における平均体重、平均体長、平均増重率、飼料効率などは、脱脂酵素処理魚粉68%、スジコ油8%およびα化デンプン3%の飼料3と、脱脂酵素処理魚粉63.8%、スジコ油7.54%およびα化デンプン8%を配合した飼料6を給与した区が同等で優れていた。しかし、α化デンプン配合割合を13および18%と増加させた7および8区で飼育成績は低下した。例えば、飼料効率についてみると、飼料6区が136%、次いで3区127%、7区122%および8区120%の順に低下し、6区と3区の間に有意な区間差はなかったが、6区と7・8区の間に有意差(危険率5%)があった。
(2) Rearing results Table 8 shows the rearing results for 8 days. The average body weight, average body length, average weight increase rate, feed efficiency, etc. at the end were as follows: feed 3 of defatted enzyme-treated fish meal 68%, sudiko oil 8% and pregelatinized starch 3%, and defatted enzyme-treated fish meal 63.8%, The group fed with feed 6 containing 7.54% sudiko oil and 8% pregelatinized starch was equivalent and excellent. However, the breeding performance decreased in 7 and 8 sections where the pregelatinized starch content was increased to 13 and 18%. For example, in terms of feed efficiency, feed 6 ward decreased in order of 136%, then 3 ward 127%, 7 ward 122% and 8 ward 120%, and there was no significant difference between 6 ward and 3 ward. However, there was a significant difference (risk rate 5%) between 6 wards and 7.8 wards.
一方、表中の生残率をみると、飼料8区が最も高く60.2%であったが、飼料3、6および7区が50%前後で区間差は僅かであった。なお、これらは衝突死が主因であり、魚体が大きくなると頻発することが示されている。
飼育成績が飼料3及び6区で優れていたことから、マグロ属魚類用飼料中のα化デンプンの特に好ましい配合割合は、8%、或いはそれ以下である。
On the other hand, looking at the survival rate in the table, the feed 8 ward was the highest at 60.2%, but the feed 3, 6 and 7 wards were around 50%, and the difference between the sections was slight. In addition, these are mainly caused by collision death, and it has been shown that they frequently occur when the fish size increases.
Since the breeding performance was excellent in feeds 3 and 6, the particularly preferred blending ratio of pregelatinized starch in the feed for fish of the genus Tuna is 8% or less.
前述の試験例を総合評価すると、特に適正な飼料タンパク質:脂質:糖質含量比(重量比)は、65〜80:4〜12:3〜10であり、詳細には、62〜74:8〜12:3〜8である。即ち、重量比が12:3:2前後となるようにタンパク質:脂質:糖質を組み合わせることにより優れた飼育成績を生む飼料を製造することができる。
また、試験例2および3より、エネルギー含量が5190〜5300Kcal/kg飼料(21700〜22170kJ/kg飼料)によって格別優れた飼育成績が得られることが明らかとなった。
When the above test examples are comprehensively evaluated, the particularly suitable feed protein: lipid: sugar content ratio (weight ratio) is 65 to 80: 4 to 12: 3 to 10, and specifically 62 to 74: 8. ~ 12: 3-8. That is, a feed that produces excellent breeding results can be produced by combining protein: lipid: saccharide such that the weight ratio is around 12: 3: 2.
In addition, from Test Examples 2 and 3, it was revealed that exceptionally excellent rearing results can be obtained with an energy content of 5190-5300 Kcal / kg feed (21700-22170 kJ / kg feed).
<試験例4>マグロ属魚類用飼料の有効性に関する試験
本発明の有効性を確認するために,試験例2および3と同一起源のクロマグロ稚魚を供試して、飼料6とイカナゴによる飼育試験を行った。
<Test Example 4> Test on effectiveness of tuna fish feed In order to confirm the effectiveness of the present invention, a bluefin tuna fry of the same origin as in Test Examples 2 and 3 was tested, and a feed test using feed 6 and squid was conducted. went.
(1)供試魚および飼育方法
近畿大学水産養殖種苗センター浦神事業場で人工種苗生産した平均体重9.5gのクロマグロ稚魚を、40トンコンクリート製円形水槽に145尾ずつ収容して2試験区を設けた。各水槽には60L/minで濾過海水を注水した。期間中の水温は26.3〜26.9℃、溶存酸素料は6.02〜7.32mg/Lであった。
表9に示した試験飼料組成に従い、試験飼料を作成した。原料を良く混合してから外割で10%の水道水を加え、試験用造粒機で直径3.0mmのペレットに調製した。飼育期間は11日間とし毎日6:00、10:00、14:00および18:00に、飼料6とイカナゴ切餌を所定の試験区に飽食給与した。
(1) Test Fish and Breeding Method 145 fish bluefin tuna with an average weight of 9.5 g produced by artificial seedling production at the Kinki University Aquaculture Seedling Center Uragami Plant, each containing 145 fish in a 40-ton concrete circular aquarium. Provided. Filtered seawater was poured into each water tank at 60 L / min. The water temperature during the period was 26.3 to 26.9 ° C., and the dissolved oxygen content was 6.02 to 7.32 mg / L.
A test feed was prepared according to the test feed composition shown in Table 9. After mixing the raw materials well, 10% tap water was added in an external ratio and prepared into a 3.0 mm diameter pellet with a test granulator. The breeding period was 11 days, and at 6: 00, 10: 00, 14: 00 and 18: 00 daily, feed 6 and cuttlefish were fed to a predetermined test area.
(2)飼育成績
11日間の飼育成績を表10に示す。終了時における平均体重、平均体長、増重率、飼料効率などは、飼料6区では45.9g、14.6cm、340%および143%、イカナゴ区では46.7g、14.5cm、362%および118%であった。平均体重、体長および増重率に顕著な差異はなかったが、飼料効率は飼料6区がイカナゴ区より優れていた。
(2) Rearing results Table 10 shows the rearing results for 11 days. Average body weight, average body length, weight gain, feed efficiency, etc. at the end were 45.9 g, 14.6 cm, 340% and 143% in the 6th feed, 46.7g, 14.5cm, 362% in the Ikanago district and It was 118%. Although there was no significant difference in average body weight, body length, and weight gain rate, feed efficiency was better in the 6th district than in the Ikanago district.
表中の生残率をみると、しかしながら、飼料6区が66.1%であり、イカナゴ区の81.8%より顕著に劣った。これは、飼料6区ではイカナゴ区より遊泳活動が活発で、斃死魚には頭部の変形と脊椎骨の骨折が主に認められたことから、飼料組成に問題があるのでなく、単に衝突死が頻繁に生じた結果と考えられる。一方、イカナゴ区では比較的小さくかつやせた個体が多く斃死し、消化管に食塊も認められなかったことから、摂餌不活発によるものと推察される。なお、実際の種苗生産現場では、試験例1〜4で供試した大きさのクロマグロ稚魚は、海面に設置した直径あるいは一辺が8〜12mの網生簀で飼育することから、衝突死の発生は顕著に低くなる。 Looking at the survival rate in the table, however, the feed 6 ward was 66.1%, which was significantly inferior to the 81.8% of the Ikanago ward. This is because, in the feed 6 ward, the swimming activity was more active than the Ikanago ward, and the dying fish mainly showed head deformation and vertebral fractures, so there was no problem with the feed composition, and there was simply a collision death. This is probably the result of frequent occurrences. On the other hand, in Ikanago Ward, many relatively small and thin individuals were drowned and no bolus was observed in the digestive tract. In actual seed and seedling production sites, the bluefin tuna larvae of the size tested in Test Examples 1 to 4 are bred in a net ginger with a diameter or side of 8 to 12 m installed on the sea surface. Remarkably lower.
飼料6区の飼育成績がイカナゴ切餌のそれに匹敵していたことから、本発明のマグロ属魚類用飼料が、体重50gまでのマグロ属魚類の稚魚に対して、特に好適に使用されることが示唆された。 Since the breeding performance of the feed 6 wards was comparable to that of cuttlefish, the tuna fish feed of the present invention is particularly preferably used for juvenile tuna fish up to 50 g in weight. It was suggested.
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011158363A JP2011200260A (en) | 2011-07-19 | 2011-07-19 | Feed for fish of thunnus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011158363A JP2011200260A (en) | 2011-07-19 | 2011-07-19 | Feed for fish of thunnus |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006341897A Division JP5131618B2 (en) | 2006-12-19 | 2006-12-19 | Feed for fry of tuna fish |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2011200260A true JP2011200260A (en) | 2011-10-13 |
Family
ID=44877699
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011158363A Pending JP2011200260A (en) | 2011-07-19 | 2011-07-19 | Feed for fish of thunnus |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2011200260A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013188151A (en) * | 2012-03-12 | 2013-09-26 | Hayashikane Sangyo Kk | Method of manufacturing fish-breeding feed and fish-breeding feed manufactured by the same |
CN105360721A (en) * | 2015-11-24 | 2016-03-02 | 广州聚注专利研发有限公司 | Tuna special feed and preparation method thereof |
CN113727610A (en) * | 2019-01-28 | 2021-11-30 | 斯克雷廷水产养殖研究中心公司 | Feed for aquatic organisms having stable, soft and elastic texture |
CN116998634A (en) * | 2023-08-28 | 2023-11-07 | 三亚热带水产研究院 | Artificial feed for tuna in yellow fins and domestication and feeding method |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61234747A (en) * | 1985-04-10 | 1986-10-20 | Yasuzo Uchida | Feed for fry |
JP2001161281A (en) * | 1999-12-06 | 2001-06-19 | Naohiko Sato | Feed additive for animals in industry |
JP2002058435A (en) * | 2000-08-15 | 2002-02-26 | Hokkaido Univ | Feed containing docosahexaenoic acid and/or conjugated- type docosahexaenoic acid and method for culturing fish using the same |
JP2003516714A (en) * | 1999-04-21 | 2003-05-20 | アンスティテュ ナスィヨナル ドゥ ラ ルシェルシュ アグロナミーク | Complete feed for fry and its production method |
JP2004337003A (en) * | 2002-04-18 | 2004-12-02 | Showa Denko Kk | Solid feed for cultured fish |
JP2005027613A (en) * | 2003-07-11 | 2005-02-03 | Hayashikane Sangyo Kk | Mixed feed for cultured fish |
JP2006223164A (en) * | 2005-02-16 | 2006-08-31 | Univ Kinki | Feed for fish of thunnus, biting promoting substance for fish of thunnus, and false feed for fishing fish of thunnus |
-
2011
- 2011-07-19 JP JP2011158363A patent/JP2011200260A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61234747A (en) * | 1985-04-10 | 1986-10-20 | Yasuzo Uchida | Feed for fry |
JP2003516714A (en) * | 1999-04-21 | 2003-05-20 | アンスティテュ ナスィヨナル ドゥ ラ ルシェルシュ アグロナミーク | Complete feed for fry and its production method |
JP2001161281A (en) * | 1999-12-06 | 2001-06-19 | Naohiko Sato | Feed additive for animals in industry |
JP2002058435A (en) * | 2000-08-15 | 2002-02-26 | Hokkaido Univ | Feed containing docosahexaenoic acid and/or conjugated- type docosahexaenoic acid and method for culturing fish using the same |
JP2004337003A (en) * | 2002-04-18 | 2004-12-02 | Showa Denko Kk | Solid feed for cultured fish |
JP2005027613A (en) * | 2003-07-11 | 2005-02-03 | Hayashikane Sangyo Kk | Mixed feed for cultured fish |
JP2006223164A (en) * | 2005-02-16 | 2006-08-31 | Univ Kinki | Feed for fish of thunnus, biting promoting substance for fish of thunnus, and false feed for fishing fish of thunnus |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013188151A (en) * | 2012-03-12 | 2013-09-26 | Hayashikane Sangyo Kk | Method of manufacturing fish-breeding feed and fish-breeding feed manufactured by the same |
CN105360721A (en) * | 2015-11-24 | 2016-03-02 | 广州聚注专利研发有限公司 | Tuna special feed and preparation method thereof |
CN113727610A (en) * | 2019-01-28 | 2021-11-30 | 斯克雷廷水产养殖研究中心公司 | Feed for aquatic organisms having stable, soft and elastic texture |
JP2022523067A (en) * | 2019-01-28 | 2022-04-21 | スクレッティング アクアカルチャー リサーチ センター アーエス | Feed for aquatic seeds with a stable, soft and elastic texture |
JP7494189B2 (en) | 2019-01-28 | 2024-06-03 | スクレッティング アクアカルチャー リサーチ センター アーエス | Feed for aquatic species with a stable, soft and chewy texture |
CN116998634A (en) * | 2023-08-28 | 2023-11-07 | 三亚热带水产研究院 | Artificial feed for tuna in yellow fins and domestication and feeding method |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Waiho et al. | Larval rearing of mud crab (Scylla): What lies ahead | |
Harel et al. | Advanced DHA, EPA and ArA enrichment materials for marine aquaculture using single cell heterotrophs | |
Ajiboye et al. | A review of the use of copepods in marine fish larviculture | |
JP2008220180A (en) | Artificial formula feed for fish of thunnus, and rearing method of fish of thunnus | |
Holme et al. | Survival, development and growth response of mud crab, Scylla serrata, megalopae fed semi-purified diets containing various fish oil: corn oil ratios | |
WO2016065231A1 (en) | Aquaculture feed formulation and aquaculture product produced with the same | |
JP2004057156A (en) | Feed for pisciculture | |
Mukhopadhyay et al. | Applied nutrition in freshwater prawn, Macrobrachium rosenbergii, culture | |
JP5131618B2 (en) | Feed for fry of tuna fish | |
AU2015201193A1 (en) | Feed product and/or feed ingredient | |
RU2660264C2 (en) | Selection and production of whole algae as a food additive for the large cattle and bisons feeding to produce meat with useful for human health high omega-3 content | |
JP2011200260A (en) | Feed for fish of thunnus | |
Khanjani et al. | Broodstock and seed production in biofloc technology (BFT): an updated review focused on fish and penaeid shrimp | |
Yeap et al. | Barriers in European spiny lobster (Palinurus elephas) aquaculture: What we know so far? | |
Holme et al. | Use of microbound diets for larval culture of the mud crab, Scylla serrata | |
JP4046239B2 (en) | Tuna fish feeding facilitator, tuna fish formula feed and simulated food for catching tuna fish | |
Muralisankar et al. | Effects of dietary copper on the growth physiology and biochemistry of the freshwater prawn Macrobrachium rosenbergii post larvae | |
JPWO2010010794A1 (en) | Survival limit temperature tolerance imparting agent for seafood and method for culturing seafood | |
Gao et al. | Microalgae as fishmeal alternatives in aquaculture: current status, existing problems, and possible solutions | |
Soltan et al. | Effect of some over-wintering regimes on survival and growth performance of Nile tilapia Oreochromis niloticus | |
Ali et al. | Impact of formulated diets on the growth and survival of ornamental fish Pterophyllum scalare (Angel Fish) | |
Grassi et al. | Growth performance and flesh quality of tilapia (Oreochromis niloticus) fed low concentrations of Rubrivivax gelatinosus, Saccharomyces cerevisiae and Spirulina platensis | |
Chang et al. | Aurantiochytrium dietary supplements reduce intra-cohort cannibalism among orange-spotted groupers (Epinephelus coioides) by modulating brain 5-HT and serum cortisol | |
Agouz et al. | Effect of feed supplementation with L-carnitine on growth and cold tolerance of the Nile tilapia, Oreochromis niloticus | |
CN102379376A (en) | Forage capable of improving broodstock reproductive performance of Cherax quadricarionatus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20110818 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20130703 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20131106 |