JP2010523987A - 個々の輸送体の蛍光分析用バイオチップ - Google Patents
個々の輸送体の蛍光分析用バイオチップ Download PDFInfo
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- JP2010523987A JP2010523987A JP2010502410A JP2010502410A JP2010523987A JP 2010523987 A JP2010523987 A JP 2010523987A JP 2010502410 A JP2010502410 A JP 2010502410A JP 2010502410 A JP2010502410 A JP 2010502410A JP 2010523987 A JP2010523987 A JP 2010523987A
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- biochip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
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- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Optical Measuring Cells (AREA)
Abstract
Description
2 胞
10 担体
15 生物学的細胞
20 層
21 層開口
22 金属層
30 凹所
31 凹所開口
35 凹所群
36 アレイ
40 膜
50 輸送分子
60 基盤
70 輸送
80 励起光
Claims (10)
- 個々の輸送システム(50)の特性を光で測定するためのバイオチップ(1)が、実質的に、1つの透明な担体(10)及び上方へ開く複数の凹所(30)を含み、バイオチップ(1)の開口(31)が膜(40)によって覆われ、こうして閉じた測定室(30)が形成され、膜(40)を経て凹所(30)への基板分子(60)の輸送が検出可能であるように、バイオチップ(1)が構成されている、バイオチップ。
- 担体(10)が大きい屈折率を持つ材料特にガラス、珪素又は酸化珪素から成り、この屈折率が使用される測定溶液の屈折率より大きいことを特徴とする、請求項1に記載のバイオチップ。
- 担体(10)が、その上側に結合される1つ又は複数の層(20)を持っていることを特徴とする、請求項1又は2に記載のバイオチップ。
- 上方へ開く凹所(30)が1つの層又は複数の層(20)に設けられていることを特徴とする、先行する請求項の1つに記載のバイオチップ。
- 凹所(30)の直径が励起光(80)の波長より小さく、従って凹所(30)がゼロモード導波路として構成されていることを特徴とする、先行する請求項の1つに記載のバイオチップ。
- 膜(40)及びその上にある基板分子(60)から励起光(80)を遮蔽するために、層(20)の少なくとも1つが光を透過しない材料特に金属なるべく金又はチタンから成っていることを特徴とする、先行する請求項の1つに記載のバイオチップ。
- 層(20)の1つ又は複数が、結合材により担体(10)に結合され、結合材がなるべくクロム又はチタンであることを特徴とする、先行する請求項の1つに記載のバイオチップ。
- 励起光(80)を反射して基板分子(60)を繰り返し励起するため、層(20)がその下側で反射するように構成されていることを特徴とする、先行する請求項の1つに記載のバイオチップ。
- バイオチップ(1)の表面が、特にアミノ反応するリンカ分子及び/又は膜(40)をチップ(1)に結合する脂質誘導体を持っていることを特徴とする、先行する請求項の1つに記載のバイオチップ。
- 膜(40)を電気的に測定するか又は電気的に制御又は開閉するため、層(20)が導電性で電極として構成されていることを特徴とする、先行する請求項の1つに記載のバイオチップ。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102007016699.2 | 2007-04-04 | ||
DE102007016699A DE102007016699A1 (de) | 2007-04-04 | 2007-04-04 | Biochip für die Fluoreszenzanalyse von einzelnen Transportern |
PCT/DE2008/000532 WO2008122267A2 (de) | 2007-04-04 | 2008-04-02 | Biochip für die fluoreszenzanalyse von einzelnen transportern |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2010523987A true JP2010523987A (ja) | 2010-07-15 |
JP5370864B2 JP5370864B2 (ja) | 2013-12-18 |
Family
ID=39731117
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2010502410A Expired - Fee Related JP5370864B2 (ja) | 2007-04-04 | 2008-04-02 | 個々の輸送体の蛍光分析用バイオチップ |
Country Status (5)
Country | Link |
---|---|
US (1) | US20100092341A1 (ja) |
EP (1) | EP2142911A2 (ja) |
JP (1) | JP5370864B2 (ja) |
DE (1) | DE102007016699A1 (ja) |
WO (1) | WO2008122267A2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013540986A (ja) * | 2010-07-12 | 2013-11-07 | ナノスポツト・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | 人工膜又は生体膜の特性の光学測定のための微小構造化された測定チップ及びその製造のための方法 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102007059166A1 (de) * | 2007-12-06 | 2009-06-10 | Synentec Gmbh | Vorrichtung zur Messung von Transportsystemen |
US20120050734A1 (en) * | 2009-04-15 | 2012-03-01 | Stefan Wennmalm | Inverse-fluorescence correlation spectroscopy |
US20130099143A1 (en) * | 2010-06-30 | 2013-04-25 | Tosoh Corporation | Structure for particle immobilization and apparatus for particle analysis |
DE102014212695B4 (de) | 2013-07-18 | 2018-10-25 | Amo Gmbh | Verfahren zur Herstellung von Kavitäten mit nanoskaligen Blenden |
WO2015110614A1 (en) * | 2014-01-23 | 2015-07-30 | Imec Vzw | Integrated waveguide structure for fluorescence analysis |
JP6607936B2 (ja) * | 2015-06-08 | 2019-11-20 | 国立研究開発法人科学技術振興機構 | 高密度微小チャンバーアレイおよびこれを用いた測定方法 |
CN113607714B (zh) * | 2021-10-08 | 2022-01-11 | 成都齐碳科技有限公司 | 分子膜成膜或表征器件、装置、方法以及生物芯片 |
Citations (7)
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US20030174992A1 (en) * | 2001-09-27 | 2003-09-18 | Levene Michael J. | Zero-mode metal clad waveguides for performing spectroscopy with confined effective observation volumes |
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JP2006506642A (ja) * | 2002-11-20 | 2006-02-23 | コーニング インコーポレイテッド | 生体膜アレイのための安定した表面化学性質を有する基体およびその製造方法 |
WO2006044078A2 (en) * | 2004-09-17 | 2006-04-27 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
US7144486B1 (en) * | 1997-04-30 | 2006-12-05 | Board Of Trustees Of The University Of Arkansas | Multilayer microcavity devices and methods |
WO2006136991A1 (en) * | 2005-06-23 | 2006-12-28 | Koninklijke Philips Electronics N.V. | Luminescence sensors using sub-wavelength apertures or slits |
JP2009539411A (ja) * | 2006-06-12 | 2009-11-19 | パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド | 分析反応を行うための基板 |
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2007
- 2007-04-04 DE DE102007016699A patent/DE102007016699A1/de not_active Withdrawn
-
2008
- 2008-04-02 EP EP08748708A patent/EP2142911A2/de not_active Withdrawn
- 2008-04-02 US US12/450,093 patent/US20100092341A1/en not_active Abandoned
- 2008-04-02 JP JP2010502410A patent/JP5370864B2/ja not_active Expired - Fee Related
- 2008-04-02 WO PCT/DE2008/000532 patent/WO2008122267A2/de active Application Filing
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US20030174992A1 (en) * | 2001-09-27 | 2003-09-18 | Levene Michael J. | Zero-mode metal clad waveguides for performing spectroscopy with confined effective observation volumes |
JP2006505278A (ja) * | 2002-11-07 | 2006-02-16 | コーニング・インコーポレーテッド | 細胞などの対象物の標識フリー検出を可能にする多層凹状体組込み装置 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2013540986A (ja) * | 2010-07-12 | 2013-11-07 | ナノスポツト・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | 人工膜又は生体膜の特性の光学測定のための微小構造化された測定チップ及びその製造のための方法 |
Also Published As
Publication number | Publication date |
---|---|
US20100092341A1 (en) | 2010-04-15 |
DE102007016699A1 (de) | 2008-10-09 |
JP5370864B2 (ja) | 2013-12-18 |
WO2008122267A2 (de) | 2008-10-16 |
EP2142911A2 (de) | 2010-01-13 |
WO2008122267A3 (de) | 2008-12-11 |
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