JP2010142128A - Mixed feed for puffer and method for culturing puffer - Google Patents

Mixed feed for puffer and method for culturing puffer Download PDF

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JP2010142128A
JP2010142128A JP2008319509A JP2008319509A JP2010142128A JP 2010142128 A JP2010142128 A JP 2010142128A JP 2008319509 A JP2008319509 A JP 2008319509A JP 2008319509 A JP2008319509 A JP 2008319509A JP 2010142128 A JP2010142128 A JP 2010142128A
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Kotaro Kikuchi
弘太郎 菊池
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Central Research Institute of Electric Power Industry
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Abstract

<P>PROBLEM TO BE SOLVED: To obtain new mixed feed for puffer, promoting growth of puffer represented by tiger puffer. <P>SOLUTION: The mixed feed contains free alanine as the growth promoting component. The content of free alanine is at least 0.06% based on the feed total weight. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

本発明は、フグ類配合飼料及びフグ類養殖方法に関する。さらに詳述すると、本発明は、トラフグに代表されるフグ類の成長を促進するのに好適なフグ類用配合飼料及びフグ類養殖方法に関する。   The present invention relates to a puffer fish-containing feed and a puffer fish culture method. More specifically, the present invention relates to a pufferfish mixed feed suitable for promoting the growth of pufferfish typified by trough pufferfish and a puffer culture method.

養魚用配合飼料のタンパク質源としては、従来、真鰯やスケトウダラといった多獲性魚類を原料とした魚粉が用いられていた。しかし、近年、多獲性魚類の漁獲量の減少と養殖業の拡大による配合飼料の需要の増加により、魚粉の供給が逼迫するとともにその価格も高騰し、魚粉のみでは養魚用配合飼料の需要がまかなえなくなってきている。そこで、魚粉の一部を安価なタンパク質源である植物性タンパク質で代替することが検討されている。   As a protein source for a mixed feed for fish farming, fish meal made from high-yielding fish such as red snapper and walleye pollen has been conventionally used. However, in recent years, due to a decrease in the catch of high-capacity fish and an increase in the demand for mixed feed due to the expansion of the aquaculture industry, the supply of fish meal has become tight and the price has soared. It's getting out of control. Therefore, replacement of a part of fish meal with vegetable protein, which is an inexpensive protein source, has been studied.

植物性タンパク質を含む原料としては、大豆粕やコーングルテンミール、菜種油滓等といったものが挙げられ、これらは、生産量も豊富で価格も安定していることから魚粉の代替材料として有望視されている。   Examples of raw materials containing vegetable protein include soybean meal, corn gluten meal, rapeseed oil meal, etc. These are promising as substitutes for fish meal because of their abundant production and stable prices. Yes.

しかし、魚粉の一部を植物性タンパク質で代替した配合飼料を使用して養殖を行うと、ある特有の問題が生じる。即ち、魚粉の一部を植物性タンパク質で代替した配合飼料は、嗜好性が低く、養殖魚の摂餌量が低下して成長低下を招く。このことは多くの魚種で明らかとなっている。したがって、配合飼料中の魚粉の一部を植物性タンパク質で代替する場合、養殖魚の成長低下を抑えるために何らかの手段を講じる必要性が生じる。   However, there is a particular problem when culturing using a mixed feed in which part of the fish meal is replaced with vegetable protein. That is, a mixed feed obtained by substituting a part of fish meal with a vegetable protein has low palatability, and the amount of feed of the cultured fish is reduced, leading to a decrease in growth. This is evident in many fish species. Therefore, when a part of the fish meal in the blended feed is replaced with plant protein, it is necessary to take some measures to suppress the growth reduction of the cultured fish.

例えば、非特許文献1では、ムラサキイガイ軟体部粉末乾燥を飼料に配合することで、飼料中の魚粉の一部を脱皮大豆粕(SBM−P)で置換した場合にも、ヒラメの成長が増大することが報告されている。   For example, in Non-Patent Document 1, the growth of Japanese flounder increases even when a portion of fish meal in the feed is replaced with molted soybean meal (SBM-P) by blending dried mussel soft part powder into the feed. It has been reported.

K. Kikuchi. Use of defatted soybean meal as substitutes for fish meal in diets of Japanese flounder (Paralichthys olivaceus). Aquaculture 1999; 179: 3-11.K. Kikuchi. Use of defatted soybean meal as substitutes for fish meal in diets of Japanese flounder (Paralichthys olivaceus). Aquaculture 1999; 179: 3-11.

ところで、トラフグに代表されるフグ類は市場価値の高い所謂高級魚であり、漁獲量が不安定なことから、市場への安定供給が可能な養殖業への期待が大きい。しかしながら、上記の通り、近年、魚粉のみでは養魚用配合飼料の需要がまかなえなくなってきていることから、今後、タンパク質源を全て魚粉としている従来の配合飼料は安定且つ安価には供給できなくなる虞がある。したがって、魚粉の一部を植物性タンパク質で代替した場合にも、フグ類の成長を促進することのできる新たな配合飼料の開発が望まれている。   By the way, puffer fish represented by trough puffer fish are so-called high-quality fish with high market value, and their catch is unstable, so there is a great expectation for aquaculture industry that can stably supply to the market. However, as described above, in recent years, since the demand for fish feed is no longer met with fish meal alone, there is a possibility that the conventional compound feed containing all the protein source as fish meal cannot be supplied stably and inexpensively in the future. is there. Therefore, even when a part of fish meal is replaced with vegetable protein, development of a new mixed feed that can promote the growth of puffer fish is desired.

そこで、本発明は、トラフグに代表されるフグ類の成長を促進することのできる新規なフグ類用配合飼料及びこれを用いたフグ類の養殖方法を提供することを目的とする。   Accordingly, an object of the present invention is to provide a novel blended feed for puffer fish that can promote the growth of puffer fish represented by trough puffer fish, and a puffer fish culture method using the same.

本願発明者は、トラフグを対象とした養殖試験を行う中で、タンパク態のアミノ酸ではない遊離アラニンがトラフグの成長促進成分として機能することを知見し、本願発明を完成するに至った。   The inventor of the present application has found that free alanine, which is not a protein amino acid, functions as a growth promoting component of trough puffer fish while conducting a culture test for trough puffer fish, and has completed the present invention.

即ち、本発明のフグ類用配合飼料は、遊離アラニンを成長促進成分として含むものである。   That is, the blended feed for puffer fish of the present invention contains free alanine as a growth promoting component.

また、本発明のフグ類配合飼料において、遊離アラニンの含有量を飼料総重量に対し少なくとも0.06%とすることが好ましい。   Moreover, in the puffer fish-containing feed of the present invention, the content of free alanine is preferably at least 0.06% with respect to the total weight of the feed.

また、本発明のフグ類養殖方法は、飼料と共に遊離アラニンをフグ類に摂取させることにより、フグ類の成長を促進させるようにしている。   In addition, the puffer fish culture method of the present invention promotes the growth of puffer fish by allowing the puffer fish to ingest free alanine together with the feed.

本発明のフグ類配合飼料及びフグ類養殖方法によれば、フグ類の成長を促進させることができる。したがって、魚粉の一部を植物性タンパク質で代替した場合にも、フグ類の成長状態を良好なものとすることができる。   According to the puffer fish-containing feed and puffer culture method of the present invention, the growth of puffer fish can be promoted. Therefore, even when part of the fish meal is replaced with plant protein, the growth state of puffers can be improved.

以下、本発明を実施するための最良の形態について説明する。   Hereinafter, the best mode for carrying out the present invention will be described.

本発明のフグ類用配合飼料は、遊離アラニンを成長促進成分として含むものである。遊離アラニンを成長促進成分として含むことにより、トラフグ、マフグ、ショウサイフグ、ヒガンフグ、クサフグ、サバフグ等のフグ類全般、特にトラフグの成長を促進する効果が発揮される。   The puffer's blended feed of the present invention contains free alanine as a growth promoting component. By including free alanine as a growth promoting component, the effect of promoting the growth of trough pufferfish in general, such as trough pufferfish, pufferfish, shosaipugu pufferfish, puffer pufferfish, kusafugu, mackerel pufferfish.

本発明のフグ類配合飼料では、遊離アラニンを含む原料あるいは結晶アラニンを飼料に配合することによって、飼料に遊離アラニンを含ませるようにしている。遊離アラニンを含む原料としては、例えばムラサキイガイが挙げられる。ムラサキイガイには遊離アラニンが多量に含まれていることから、その軟体部やエキスを飼料に配合することによって、飼料に遊離アラニンを含ませることができる。尚、ムラサキイガイを利用することで、従来廃棄物とされていたムラサキイガイを有効利用することが可能となる。即ち、海岸の堤防や発電所の取水口、復水器などに付着した多量のムラサキイガイは、これらの設備の機能を低下させるものであることから、除去・回収する必要があり、その年間処理量は2万トン(殻付き新鮮重量)以上と大量であるが(火力原子力発電技術協会 環境対策技術調査委員会(2003):火力発電所における海洋生物対策実態調査報告書、158pp.)、遊離アラニンを含む原料としてムラサキイガイを利用することで、ムラサキイガイの処分上の問題の解決に寄与することができることとなる。しかも、従来廃棄物とされていたムラサキイガイを原料としていることから、飼料にかかる原料費を抑えることもできる。また、上記の通り、ムラサキイガイの年間処理量は2万トン(殻付き新鮮重量)と大量であることから、原料を長期に亘って安定供給することもできる。したがって、安定且つ安価に配合飼料を供給することも可能となる。但し、遊離アラニンを含む原料は、ムラサキイガイに限定されるものではなく、ムラサキイガイ以外の他の原料をフグ類の成長に悪影響を与えない範囲で適宜選択することができる。   In the puffer fish-containing feed of the present invention, the feed contains free alanine by mixing a raw material containing free alanine or crystalline alanine with the feed. Examples of the raw material containing free alanine include mussel. Since mussels contain a large amount of free alanine, free alanine can be included in the feed by blending the soft body part and the extract with the feed. By using mussels, it is possible to effectively use mussels that have been conventionally disposed of as waste. That is, a large amount of blue mussels adhering to coastal dikes, power plant intakes, condensers, etc. will deteriorate the function of these facilities, so it is necessary to remove and collect them. Is a large amount of over 20,000 tons (fresh weight with shell) (Environmental Technology Research Committee for Thermal Power and Nuclear Power Technology (2003): Survey report on marine life countermeasures at thermal power plants, 158 pp.), Free alanine By using mussels as a raw material containing mussel, it is possible to contribute to the solution of mussels disposal problems. Moreover, since mussels that have been conventionally regarded as waste are used as raw materials, the raw material costs for feed can be reduced. In addition, as described above, the annual processing amount of blue mussels is as large as 20,000 tons (fresh weight with shells), so that the raw materials can be stably supplied over a long period of time. Therefore, it becomes possible to supply the mixed feed stably and inexpensively. However, the raw material containing free alanine is not limited to blue mussels, and other raw materials other than blue mussels can be appropriately selected within a range that does not adversely affect the growth of puffers.

本発明のフグ類用配合飼料中の遊離アラニン含有量は、飼料総重量に対し少なくとも0.06%とすることで確実に成長促進効果が得られるが、0.02%超、好ましくは0.04%以上とすれば一定の成長促進効果が得られるものと推定される。0.02%以下とすると、フグ類に対し十分な成長促進成分を与えることができない場合がある。尚、遊離アラニンの含有量が1%を超えても、遊離アラニンによるフグ類に対する成長促進効果が低下することは無いが、遊離アラニンの含有量を多くし過ぎると配合飼料中における遊離アラニンの占める割合が大きくなり、フグ類の成長に必要な成分を十分に含有させることができなくなる場合がある。また、遊離アラニンの含有量を1%とすれば、十分な成長促進効果が得られ、それ以上に遊離アラニンの含有量を増やしても大幅な成長促進効果の向上は見込めないものと考えられる。したがって、遊離アラニンの含有量は、多くとも1%程度とすれば十分である。このように、本発明によれば、遊離アラニンという特定の物質を少量含ませるだけで、フグ類に対する成長促進効果を得ることができる。したがって、フグ類に必要な成分を飼料中に十分に配合しながらも同時にフグ類の成長を促進することのできる極めて優れたフグ類用配合飼料を提供することができる。   The content of free alanine in the blended feed for puffer fish of the present invention is surely at least 0.06% based on the total weight of the feed, so that a growth promoting effect can be surely obtained. If it is 04% or more, it is estimated that a certain growth promoting effect can be obtained. If the content is 0.02% or less, a sufficient growth promoting component may not be provided to the puffer fish. In addition, even if content of free alanine exceeds 1%, the growth promotion effect with respect to puffers by free alanine does not fall, but if content of free alanine is increased too much, free alanine in the mixed feed will occupy In some cases, the ratio increases, and it is impossible to sufficiently contain components necessary for the growth of puffer fish. Further, if the content of free alanine is 1%, a sufficient growth promoting effect is obtained, and it is considered that a significant improvement in the growth promoting effect cannot be expected even if the content of free alanine is increased further. Accordingly, it is sufficient that the content of free alanine is at most about 1%. Thus, according to the present invention, a growth promoting effect on puffer fish can be obtained only by including a small amount of a specific substance called free alanine. Therefore, it is possible to provide an extremely excellent pufferfish mixed feed that can sufficiently promote the growth of pufferfish while sufficiently blending components necessary for pufferfish into the feed.

本発明のフグ類用配合飼料では、魚粉の一部を植物性タンパク質で代替した場合にも、フグ類の成長状態を良好なものとすることができる。植物性タンパク質含有原料としては、例えば、脱皮大豆粕、コーングルテンミール、菜種油滓、綿実粕、ヒマワリ粕といった植物油滓系原料が挙げられる。これらは、生産量も豊富で価格も安定しており、配合飼料に使用するのに好適であるが、植物性タンパク質含有原料はこれらに限定されるものではない。また、タンパク源を全て魚粉とした場合にも、その成長を従来よりも促進することができる。   In the mixed feed for puffer fish according to the present invention, the growth state of puffer fish can be improved even when part of the fish meal is replaced with vegetable protein. Examples of plant protein-containing raw materials include vegetable oil-based materials such as molted soybean meal, corn gluten meal, rapeseed oil meal, cottonseed meal, and sunflower meal. These are abundant in production amount and stable in price, and are suitable for use in blended feeds, but plant protein-containing raw materials are not limited to these. Moreover, also when all protein sources are fish meal, the growth can be promoted more than before.

本発明のフグ類用配合飼料に添加されるその他の成分としては特に限定されるものではなく、従来の配合飼料とほぼ同様の成分を同様の割合で配合することができる。例示すると、馬鈴薯デンプン(例えば5〜10重量%程度)、フィードオイル(例えば5〜10重量%程度)、無機化合物(例えば0.1〜2重量%程度)、ビタミン混合物(例えば1〜3重量%程度)、α化馬鈴薯デンプン(例えば2〜4重量%程度)、デキストリン(例えば1〜3重量%程度)等が挙げられるが、これに限定されるものではない。   The other components added to the puffer's blended feed of the present invention are not particularly limited, and almost the same components as the conventional blended feed can be blended in the same proportions. For example, potato starch (for example, about 5 to 10% by weight), feed oil (for example, about 5 to 10% by weight), inorganic compound (for example, about 0.1 to 2% by weight), vitamin mixture (for example, 1 to 3% by weight) Degree), pregelatinized potato starch (for example, about 2 to 4% by weight), dextrin (for example, about 1 to 3% by weight), and the like, but are not limited thereto.

本発明の配合飼料の製造法は特に限定されるものでなく、ミートチョッパー、エクストルダーなどを用い、常法により行うことができる。また、配合飼料の形状はペレット状やクランブル状として、フグ類が摂餌しやすい大きさとすればよい。   The manufacturing method of the compound feed of this invention is not specifically limited, It can carry out by a conventional method using meat chopper, an extruder, etc. Moreover, the shape of the mixed feed may be a pellet or crumble, and the size is easy for the puffer fish to eat.

上述の形態は本発明の好適な形態の一例ではあるがこれに限定されるものではなく本発明の要旨を逸脱しない範囲において種々変形実施可能である。例えば、上述の実施形態では飼料に遊離アラニンを含むフグ類用配合飼料について説明したが、本発明は、このフグ類用配合飼料を与えてフグ類を養殖する形態には限定されない。例えば、遊離アラニンを含む配合飼料をフグ類に与えることによって遊離アラニンをフグ類に摂取させるのではなく、遊離アラニンを含む原料あるいは結晶アラニンをフグ類の養殖に従来から用いられている一般的な飼料と共にフグ類に与えることによって、遊離アラニンと飼料とをフグ類に同時に摂取させるようにしてもよい。   The above-described embodiment is an example of a preferred embodiment of the present invention, but is not limited thereto, and various modifications can be made without departing from the gist of the present invention. For example, in the above-described embodiment, the mixed feed for puffer fish containing free alanine in the feed has been described. However, the present invention is not limited to the form in which the pufferfish mixed feed is fed and cultured. For example, the feed containing free alanine is not fed into the pufferfish by feeding the pufferfish with the free alanine, but the raw material containing free alanine or crystalline alanine is conventionally used for puffer fish By giving it to the pufferfish together with the feed, the pufferfish may be allowed to ingest free alanine and the feed at the same time.

以下に本発明の実施例を説明するが、本発明はこれら実施例に限られるものではない。   Examples of the present invention will be described below, but the present invention is not limited to these examples.

(実施例1)
各種配合飼料をトラフグに与え、その成長状態について観察した。尚、本実施例において、「%」とは、重量%を意味している。
Example 1
Various mixed feeds were given to the trough and observed for its growth state. In this embodiment, “%” means% by weight.

(1−1 配合飼料の組成)
実験に使用した配合飼料の組成を表1に示す。
(1-1 Composition of formulated feed)
Table 1 shows the composition of the mixed feed used in the experiment.

Figure 2010142128
Figure 2010142128

本実施例で用いた配合飼料では、魚粉(トロールミール)配合量を減らし、脱皮大豆粕(SBM−P)由来のタンパク質が総タンパク質の約30%となるように脱皮大豆粕を27%配合した。飼料Bでは魚粉をさらに5%低下させ、7.5%のムラサキイガイ軟体部乾燥粉末を、飼料Cでは外割り重量比で10%のムラサキイガイエキスを配合した。また、飼料Dでは結晶グリシン(飼料用:昭和電工製)を0.05%配合し、飼料Eでは結晶アラニン(飼料用:昭和電工製)を0.04%配合した。飼料Fでは結晶グリシン0.05%及び結晶アラニン0.04%を配合した。また、本実施例で用いた配合飼料には、馬鈴薯デンプン、フィードオイル(理研ビタミン製)、無機混合物(日本配合飼料株式会社製)、ビタミン混合物(日本配合飼料株式会社製)、α化馬鈴薯デンプン及びデキストリンをさらに配合した。エキスならびに飼料の製造は定法に従って行い、ミートチョッパーにより直径約2.2mm、長さ約3mmの円筒状に成形した。作製した飼料は乾燥後、所定量のフィードオイルを浸透させ、再度乾燥した後、実験に供するまで−35℃で保存した。表1に示した様に、粗脂肪含量は飼料区間で違いはなかったが、粗タンパク質については飼料CとDで高くなった。   In the mixed feed used in this example, the amount of fish meal (trolmeal) was reduced, and 27% of molted soybean meal was formulated so that the protein derived from molted soybean meal (SBM-P) was about 30% of the total protein. . In feed B, the fish meal was further reduced by 5%, 7.5% mussel soft body dry powder was blended, and in feed C, 10% mussel extract was blended in the external weight ratio. In feed D, 0.05% of crystalline glycine (for feed: Showa Denko) was blended, and in feed E, crystalline alanine (for feed: Showa Denko) was blended in 0.04%. Feed F was formulated with 0.05% crystalline glycine and 0.04% crystalline alanine. In addition, potato starch, feed oil (manufactured by Riken Vitamin), inorganic mixture (manufactured by Nippon Compound Feed Co., Ltd.), vitamin mixture (manufactured by Nippon Compound Feed Co., Ltd.), pregelatinized potato starch And dextrin were further blended. The extract and the feed were produced according to a conventional method, and formed into a cylindrical shape having a diameter of about 2.2 mm and a length of about 3 mm using a meat chopper. The prepared feed was dried, impregnated with a predetermined amount of feed oil, dried again, and stored at −35 ° C. until used for experiments. As shown in Table 1, the crude fat content was not different in the feed section, but the crude protein was higher in the feeds C and D.

次に、飼料A〜Fの遊離アミノ酸組成を表2に示す。   Next, Table 2 shows the free amino acid compositions of feeds A to F.

Figure 2010142128
Figure 2010142128

ムラサキイガイ軟体部とエキス配合区(飼料B及びC)では、遊離グリシンと遊離アラニンの含有量が多くなり、特に軟体部を配合した飼料Bで遊離グリシンと遊離アラニンの量が多くなった。また、飼料D及び飼料Fの遊離グリシン含有量は飼料Cとほぼ同程度であり、飼料E及び飼料Fの遊離アラニン含有量も飼料Cとほぼ同程度であった。尚、表2に示される遊離アミノ酸組成から、飼料Aの遊離アラニン含有量は飼料総重量に対し0.02%であり、飼料Eの遊離アラニン含有量は0.06%であることがわかった。   In the mussel soft body part and the extract blending group (feeds B and C), the contents of free glycine and free alanine increased, and especially in feed B blended with the soft body part, the amounts of free glycine and free alanine increased. Moreover, the free glycine content of the feed D and the feed F was almost the same as the feed C, and the free alanine content of the feed E and the feed F was also almost the same as the feed C. From the free amino acid composition shown in Table 2, it was found that the free alanine content of feed A was 0.02% with respect to the total weight of the feed, and the free alanine content of feed E was 0.06%. .

(1−2 飼育実験)
実験に用いたトラフグは、2008年5月8日に日清マリンテック株式会社(愛知県田原市)より体重約3gの個体を購入し、実験に供するまで、総水量約2000リットルの天然海水を収容した循環濾過水槽を用い、水温を20℃に調節して、市販のトラフグ用配合飼料(日清丸紅製)により飼育した。飼育実験は、2008年の5月〜7月において8週間実施した。
(1-2 breeding experiment)
The trough puffer used in the experiment was purchased on May 8, 2008 from Nisshin Marine Tech Co., Ltd. (Tahara City, Aichi Prefecture). Using the accommodated circulating filtration water tank, the water temperature was adjusted to 20 ° C., and reared with a commercially available mixed feed for trough puffer (manufactured by Nisshin Marubeni). The breeding experiment was conducted for 8 weeks in May-July of 2008.

尚、実験の際には、飼育水槽内に浮かべた容量約35リットルの網生けすに、20尾/生けす、3連/飼料区でトラフグを収容し、所定の飼料を1日2回、各飽食量を週6日で与えて8週間給餌した。   In addition, in the experiment, about 35 liters of net cage floating in the breeding aquarium, 20 fish / living, 3 trout / feed troughs are accommodated, and predetermined feed is taken twice a day. Each satiety amount was given 6 days a week and fed for 8 weeks.

(1−3 実験結果)
飼育実験の結果を表3に示す。尚、実験結果は、3連の実験結果の平均と標準偏差で示した。
(1-3 Experimental results)
Table 3 shows the results of the breeding experiment. The experimental results are shown as the average and standard deviation of triplicate experimental results.

Figure 2010142128
Figure 2010142128

「増重率」は、以下に示す数式1により計算した。
(数式1) 増重率(%)= (増重量)/(実験開始時体重)× 100
The “weight increase rate” was calculated by the following formula 1.
(Formula 1) Weight increase rate (%) = (weight increase) / (weight at the start of experiment) × 100

「比成長率」は、以下に示す数式2により計算した。
(数式2) 比成長率 = (lnW−lnW)/56 × 100
ここで、Wは実験終了時体重であり、Wは実験開始時体重である。また、数式2中の「56」という数字は、実験期間を日数で表した値である。
The “specific growth rate” was calculated according to Equation 2 shown below.
(Formula 2) Specific growth rate = (lnW t −lnW 0 ) / 56 × 100
Here, W t is the body weight at the end of the experiment, and W 0 is the body weight at the start of the experiment. Also, the number “56” in Equation 2 is a value representing the experiment period in days.

「飼料効率」は、以下に示す数式3により計算した。
(数式3) 飼料効率(%)=(増重量)/(摂餌量)× 100
“Feed efficiency” was calculated by Equation 3 shown below.
(Formula 3) Feed efficiency (%) = (weight increase) / (food intake) × 100

「タンパク質効率」は、以下に示す数式4により計算した。
(数式4) タンパク質効率 =(増重量)/(タンパク質摂取量)
“Protein efficiency” was calculated by Equation 4 shown below.
(Formula 4) Protein efficiency = (weight increase) / (protein intake)

「日間摂餌率」は、以下に示す数式5により計算した。
(数式5) 日間摂餌率(%)= {(W−W)/2×給餌日数}× 100
The “daily feeding rate” was calculated by the following formula 5.
(Formula 5) Daily feeding rate (%) = {(W t −W 0 ) / 2 × feeding days} × 100

「生残率」は、以下に示す数式6により計算した。
(数式6) 生残率(%)=(実験終了後生残数)/(実験開始時個体数)× 100
The “survival rate” was calculated according to Equation 6 shown below.
(Formula 6) Survival rate (%) = (Number of survivors after the end of the experiment) / (Number of individuals at the start of the experiment) × 100

全ての実験区において、飼育実験開始当初から終了時まで、全てのトラフグが活発に摂餌し、終了時の生残率も97〜100%と高かった。   In all experimental plots, all troughs were actively fed from the beginning to the end of the breeding experiment, and the survival rate at the end was as high as 97 to 100%.

また、飼料Aに対し、ムラサキイガイ軟体部とエキスを添加した飼料BとC、ならびに結晶アラニンを添加した飼料EとFで高い増重率及び成長率が得られた。結晶グリシンを添加した飼料Dの成長は飼料Aと同等だった。また、結晶グリシンを添加した飼料Dでは、飼料効率、タンパク質効率(PER)も他より劣ることが明らかとなった。   Moreover, with respect to the feed A, the high weight increase rate and the growth rate were obtained by the feed B and C which added the mussel soft part and the extract, and the feed E and F which added the crystalline alanine. Growth of feed D to which crystalline glycine was added was comparable to feed A. In addition, it was revealed that the feed D to which crystalline glycine was added was inferior in feed efficiency and protein efficiency (PER).

この実験結果から、遊離アラニンがトラフグの成長促進成分として機能していることが明らかとなった。一方で、遊離グリシンはトラフグの成長促進成分としては機能しないことも明らかとなった。また、遊離アラニンの含有量は飼料総重量に対し少なくとも0.06%とすればトラフグに対する成長促進効果が確実に得られることが確認されたが、遊離アラニンの含有量を0.02%超、好ましくは0.04%以上とすれば一定の成長促進効果が得られるものと推定される。そして、トラフグ以外のフグ類、例えばマフグ、ショウサイフグ、ヒガンフグ、クサフグ、サバフグ等のフグ類についても、この実験結果が当て嵌らないとの積極的な理由が存在しないことから、これらのフグ類についても、遊離アラニンを含有する配合飼料により、その成長を促進することができるものと推定される。   From this experimental result, it became clear that free alanine functions as a growth promoting component of trough puffer fish. On the other hand, it has also been clarified that free glycine does not function as a growth promoting component of trough puffer. In addition, it was confirmed that if the content of free alanine is at least 0.06% with respect to the total weight of the feed, a growth promoting effect on trough puffer can be surely obtained, but the content of free alanine exceeds 0.02%, It is presumed that a constant growth promoting effect can be obtained when the content is preferably 0.04% or more. And there is no positive reason that this experimental result does not apply to puffers other than trough puffers, such as puffer pufferfish, puffer pufferfish, puffer pufferfish, kusa pufferfish, mackerel pufferfish, etc. It is presumed that the growth can be promoted by the formulated feed containing free alanine.

次に、トラフグの血液成分を検査し、本発明の配合飼料がトラフグの健康状態に与える影響について調べた結果を表4及び表5に示す。表4は、血液のヘモグロビン含有量、ヘマトクリット値、総赤血球数、タンパク質含有量、トリグリセリド含有量及びグルコース含有量を調べた結果であり、表5は、血液の無機リン含有量、カルシウム含有量、マグネシウム含有量、ナトリウム含有量及び塩素含有量を調べた結果である。   Next, Table 4 and Table 5 show the results of examining the blood components of trough puffer and examining the influence of the mixed feed of the present invention on the health of trough puffer. Table 4 shows the results of examining blood hemoglobin content, hematocrit value, total red blood cell count, protein content, triglyceride content and glucose content, and Table 5 shows blood inorganic phosphorus content, calcium content, It is the result of having investigated magnesium content, sodium content, and chlorine content.

Figure 2010142128
Figure 2010142128

Figure 2010142128
Figure 2010142128

表4及び表5に示される結果から、グルコースをはじめとして、変動が見られた成分があったものの、飼料組成との明確な関係は認められなかった。少なくとも、トラフグの健康状態に悪影響が及ぼされないことが明らかとなった。   From the results shown in Table 4 and Table 5, although there were components that showed fluctuations including glucose, a clear relationship with the feed composition was not recognized. At least, it became clear that there was no adverse effect on the health of trough puffers.

Claims (3)

遊離アラニンを成長促進成分として含むことを特徴とするフグ類用配合飼料。   Formulated feed for puffer fish containing free alanine as a growth promoting component. 前記遊離アラニンの含有量を飼料総重量に対し少なくとも0.06%とする請求項1記載のフグ類用配合飼料。   The mixed feed for puffer fish according to claim 1, wherein the content of the free alanine is at least 0.06% based on the total weight of the feed. 飼料と共に遊離アラニンをフグ類に摂取させることにより、前記フグ類の成長を促進させることを特徴とするフグ類養殖方法。   A puffer fish culture method characterized by promoting the growth of the pufferfish by allowing the pufferfish to ingest free alanine together with the feed.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102870722A (en) * 2012-11-02 2013-01-16 海阳市黄海水产有限公司 Large scale larva breeding method for navodon septentrionalis
WO2014020925A1 (en) * 2012-08-03 2014-02-06 協和発酵バイオ株式会社 Foodstuff for cultured fish and method for promoting growth of cultured fish using same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1128061A (en) * 1997-07-10 1999-02-02 Central Res Inst Of Electric Power Ind Formula feed for cultured fish
JP2006087348A (en) * 2004-09-24 2006-04-06 Nishinippon Environmental Energy Co Inc Feed for fish culture

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1128061A (en) * 1997-07-10 1999-02-02 Central Res Inst Of Electric Power Ind Formula feed for cultured fish
JP2006087348A (en) * 2004-09-24 2006-04-06 Nishinippon Environmental Energy Co Inc Feed for fish culture

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014020925A1 (en) * 2012-08-03 2014-02-06 協和発酵バイオ株式会社 Foodstuff for cultured fish and method for promoting growth of cultured fish using same
JPWO2014020925A1 (en) * 2012-08-03 2016-07-21 協和発酵バイオ株式会社 Cultured fish feed and method for promoting the growth of cultured fish using the feed
CN102870722A (en) * 2012-11-02 2013-01-16 海阳市黄海水产有限公司 Large scale larva breeding method for navodon septentrionalis

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