JP2010101634A - Spectrophotometer - Google Patents
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Abstract
Description
本発明は、分光光度計に関する。 The present invention relates to a spectrophotometer.
溶液中の化学物質の濃度や純度を測定するために分光光度計が用いられている。特に、バイオテクノロジーの分野では、核酸や蛋白質の濃度を測定するために例えば、１μＬ程度の極微量の試料について測定が行われ、試料の濃度が吸光度にして０．０２／ｃｍ〜７０／ｃｍ程度の広範囲の測定を可能とする分光光度計が求められている。 A spectrophotometer is used to measure the concentration and purity of chemical substances in a solution. In particular, in the field of biotechnology, in order to measure the concentration of nucleic acids and proteins, for example, a very small sample of about 1 μL is measured, and the concentration of the sample is about 0.02 / cm to about 70 / cm in terms of absorbance. There is a need for a spectrophotometer that enables a wide range of measurements.
分光光度計による吸光度の測定は、試料物質がある特定の波長の光を吸収する特性を有することを利用して、その光吸収量が試料物質の濃度と光の透過する光路の長さに比例するという原理（ランバート・ベールの法則）に基づいている。
ランバート・ベールの法則は、下記式（１）および式（２）で表わされる。
Absorbance measurement using a spectrophotometer makes use of the fact that the sample substance absorbs light of a specific wavelength, and its light absorption is proportional to the concentration of the sample substance and the length of the optical path through which the light passes. It is based on the principle of doing (Lambert-Beer's law).
Lambert-Beer's law is expressed by the following equations (1) and (2).
式（１）：Ａ＝ｌｏｇ（Ｉo ／Ｉ）＝α・Ｌ・Ｃ
式（２）：Ｔ＝Ｉ／Ｉo
〔式（１）および式（２）において、Ａは試料物質の吸光度、Ｉo は入射光強度、Ｉは出射光強度、αは試料物質のモル吸光係数、Ｌは試料溶液の厚さ（ｃｍ）、Ｃは試料物質のモル濃度（ｍｏｌ／Ｌ）、Ｔは入射光強度に対する出射光強度の光の透過度である。〕
Formula (1): A = log (I o / I) = α · L · C
Formula (2): T = I / I o
[In the formulas (1) and (2), A is the absorbance of the sample substance, I o is the incident light intensity, I is the outgoing light intensity, α is the molar extinction coefficient of the sample substance, and L is the thickness (cm ), C is the molar concentration (mol / L) of the sample substance, and T is the light transmittance of the emitted light intensity with respect to the incident light intensity. ]
通常、吸光度Ａの基準となる試料溶液の厚さＬは１ｃｍであり、例えば吸光度Ａが７０の試料物質を測定すると、光の透過度Ｔは１／１０70となり、入射光強度Ｉo と出射光強度Ｉの比が途方もなく小さい値となって、試料物質の吸光度を正確に測定することが困難となる場合がある。
従って、試料溶液の厚さＬを１ｍｍ，０．２ｍｍで測定を行い、得られた吸光度をそれぞれ、Ａ1 ，Ａ0.2 とすると、基準となる試料溶液の厚さＬが１ｃｍのときの吸光度Ａは、「１０×Ａ1 」，「５０×Ａ0.2 」で表わされる。Ｌ＝０．２ｍｍのときの吸光度Ａ0.2 ≒１．４つまり、Ｔ＝１／２５で吸光度Ａ＝７０の試料物質の測定が可能となる。また、試料物質の吸光度Ａが１０以下と低いときは、Ｌ＝１ｍｍのときの吸光度Ａ1 ＝１であり、透過度Ｔ＝１／１０となり、充分に検出可能な入射光・出射光比となり、試料物質の吸光度を正確に測定することができる。
以上のように、試料溶液の厚さ（測定光路長）を変えることによって、測定試料の濃度が吸光度にして０．０２／ｃｍ〜７０／ｃｍ程度の広範囲の測定を原理的には行うことができる。
Usually, the thickness L of the sample solution serving as a reference for the absorbance A is 1 cm. For example, when a sample substance having an absorbance A of 70 is measured, the light transmittance T is 1/10 70 , and the incident light intensity I o is output. The ratio of the incident light intensity I becomes a tremendously small value, and it may be difficult to accurately measure the absorbance of the sample substance.
Therefore, when the thickness L of the sample solution is measured at 1 mm and 0.2 mm, and the obtained absorbance is A 1 and A 0.2 , the absorbance A when the thickness L of the reference sample solution is 1 cm. Are represented by “10 × A 1 ” and “50 × A 0.2 ”. Absorbance A 0.2 ≈1.4 when L = 0.2 mm In other words, it is possible to measure a sample substance having an absorbance A = 70 at T = 1/25. When the absorbance A of the sample substance is as low as 10 or less, the absorbance A 1 = 1 when L = 1 mm and the transmittance T = 1/10, which is a sufficiently detectable incident / outgoing light ratio. The absorbance of the sample substance can be accurately measured.
As described above, in principle, by changing the thickness of the sample solution (measurement optical path length), the concentration of the measurement sample can be measured in a wide range from 0.02 / cm to 70 / cm in terms of absorbance. it can.
しかしながら、試料溶液の厚さを選ぶことができて、吸光度測定を簡便に行うことができる分光光度計は未だ知られていない。
また、極微量の測定試料を試料セル中の試料収容室に確実に注入することができ、試料セルの洗浄が容易なもの、すなわち、試料セル内の前回測定時の試料残留液を完全に除去することのできる試料セルが求められている。
However, a spectrophotometer that can select the thickness of the sample solution and can easily measure the absorbance is not yet known.
In addition, an extremely small amount of measurement sample can be reliably injected into the sample storage chamber in the sample cell, and the sample cell can be easily cleaned, that is, the sample residual liquid in the sample cell is completely removed during the previous measurement. There is a need for a sample cell that can be used.
特許文献１には、投射光ファイバーと入射光ファイバーの間に挟まれた間隙に試料となる液滴を注入し、その液滴を変化させることにより、光路長を変えて広範囲の吸光度測定を可能とする技術が開示されている。
しかしながら、この測定方法は、液滴を支持するものが表面張力のみによるため、液滴の形状が不安定となるので、測定の再現性に欠けるという問題がある。また、試料を収容する容器を使用しないため、試料が蒸発するので試料物質の濃度が経時的に変化するという問題が生じる。さらに、光路長が機構的な微調整によって設定されるため、温度変化、振動、衝撃により不安定となるという欠点がある。
In Patent Document 1, a sample droplet is injected into a gap sandwiched between a projection optical fiber and an incident optical fiber, and by changing the liquid droplet, it is possible to measure a wide range of absorbance by changing the optical path length. Technology is disclosed.
However, this measurement method has a problem that the reproducibility of measurement is lacking because the shape of the droplet becomes unstable because only the surface tension supports the droplet. In addition, since the container for storing the sample is not used, the sample evaporates, so that the concentration of the sample substance changes with time. Furthermore, since the optical path length is set by mechanical fine adjustment, there is a disadvantage that the optical path length becomes unstable due to temperature change, vibration, and impact.
特許文献２には、洗浄機構を備えた試料セルを用いる分光光度計が開示されている。
しかしながら、この分光光度計は、構造が複雑であり、また、光路長が固定されているため、単一の試料セルにおいて吸光度の測定範囲が限定されてしまうという問題がある。
Patent Document 2 discloses a spectrophotometer using a sample cell having a cleaning mechanism.
However, this spectrophotometer has a complicated structure and a fixed optical path length, so that there is a problem that the measurement range of absorbance is limited in a single sample cell.
本発明は、以上のような事情を考慮してなされたものであって、その目的は、同一の測定試料について適切な条件で吸光度測定を簡便に行うことができる分光光度計を提供することにある。
また、極微量の測定試料を試料セル中の試料収容室に確実に注入することができ、試料セルの洗浄が容易な分光光度計を提供することにある。
さらに、測定試料の蒸発を防ぐことができる分光光度計を提供することにある。
The present invention has been made in view of the above circumstances, and an object thereof is to provide a spectrophotometer that can easily perform absorbance measurement under appropriate conditions for the same measurement sample. is there.
Another object of the present invention is to provide a spectrophotometer in which a very small amount of a measurement sample can be reliably injected into a sample storage chamber in a sample cell and the sample cell can be easily cleaned.
Furthermore, it is providing the spectrophotometer which can prevent evaporation of a measurement sample.
本発明の分光光度計は、第１凹所およびこの第１凹所と連通し当該第１凹所と幅の異なる第２凹所よりなる試料収容室を有する試料セルと、
第１凹所をその幅方向に測定光が通過する第１の光路と、第２凹所をその幅方向に測定光が通過する第２の光路のいずれか一方を選択する光路選択機構とを有することを特徴とする。
The spectrophotometer of the present invention comprises a sample cell having a sample storage chamber composed of a first recess and a second recess communicating with the first recess and having a width different from that of the first recess.
A first optical path through which the measurement light passes through the first recess in the width direction; and an optical path selection mechanism for selecting one of the second optical paths through which the measurement light passes through the second recess in the width direction. It is characterized by having.
また、本発明の分光光度計においては、第１凹所および第２凹所が試料セルの上面に開口する状態で形成されていることが好ましい。 Moreover, in the spectrophotometer of the present invention, it is preferable that the first recess and the second recess are formed in a state of opening on the upper surface of the sample cell.
また、本発明の分光光度計においては、第１凹所および第２凹所の深さが０．４ｍｍ以下であることが好ましい。 Moreover, in the spectrophotometer of this invention, it is preferable that the depth of a 1st recess and a 2nd recess is 0.4 mm or less.
また、本発明の分光光度計においては、試料セルが、第１凹所および第２凹所の開口の周縁部領域の上面を覆う疎水性の材質よりなるマスク層を有することが好ましい。 Moreover, in the spectrophotometer of this invention, it is preferable that a sample cell has a mask layer which consists of a hydrophobic material which covers the upper surface of the peripheral part area | region of opening of a 1st recess and a 2nd recess.
さらに、本発明の分光光度計においては、試料セルの第１凹所および第２凹所の開口を覆うための開閉可能なカバー体が設けられていることが好ましい。 Furthermore, in the spectrophotometer of the present invention, it is preferable that a cover body that can be opened and closed is provided to cover the openings of the first and second recesses of the sample cell.
本発明の分光光度計によれば、幅の大きさが異なる２つ凹所よりなる試料収容室を有する試料セルと、光路を選択する光路選択機構とを有することにより、測定光路長の異なる光路を任意に選択することができるので、同一の測定試料について適切な条件で吸光度測定を簡便に行うことができる。
従って、吸光度が大きく異なる複数の測定試料についても、試料セルの交換を必要とせず、同一の試料セルで測定することができる。
また、本発明の分光光度計によれば、試料セルにおいて、試料収容室の凹所の深さを小さい構成とすることにより、ティッシュペーパーや布等で測定試料を簡単に拭き取ることができ、試料収容室の洗浄を容易に行うことができる。
さらに、本発明の分光光度計によれば、疎水性の材質よりなるマスク層が試料セルの上面を覆う構成により、極微量の測定試料を試料収容室へ注入するときに、仮に測定試料が試料収容室から外れた場合においても、測定試料が試料収容室の外方へ流れ出ることを防ぐことができる。
さらにまた、本発明の分光光度計によれば、第１凹所および第２凹所の開口を覆うためのカバー体を有することにより、測定試料の蒸発を防ぐことができる。
According to the spectrophotometer of the present invention, an optical path having a different measurement optical path length is provided by including a sample cell having a sample storage chamber composed of two recesses having different widths and an optical path selection mechanism for selecting an optical path. Therefore, the absorbance measurement can be easily performed on the same measurement sample under appropriate conditions.
Therefore, a plurality of measurement samples having greatly different absorbances can be measured in the same sample cell without replacing the sample cell.
Further, according to the spectrophotometer of the present invention, in the sample cell, the measurement sample can be easily wiped off with a tissue paper, cloth, etc., by setting the depth of the recess of the sample storage chamber to be small. The storage chamber can be easily cleaned.
Furthermore, according to the spectrophotometer of the present invention, when a very small amount of a measurement sample is injected into the sample storage chamber by the configuration in which the mask layer made of a hydrophobic material covers the upper surface of the sample cell, the measurement sample is assumed to be a sample. Even when the sample is removed from the storage chamber, the measurement sample can be prevented from flowing out of the sample storage chamber.
Furthermore, according to the spectrophotometer of the present invention, by having the cover body for covering the openings of the first recess and the second recess, evaporation of the measurement sample can be prevented.
以下、本発明の実施形態について具体的に説明する。 Hereinafter, embodiments of the present invention will be specifically described.
図１は、本発明の分光光度計の一例における概略を示す説明図、図２は、図１における分光光度計の要部の説明用拡大斜視図、図３は、本発明に用いられる試料セルの説明用分解斜視図、図４は、本発明に用いられる試料セルの平面図、図５は、図４における試料収容室形成用板のａ−ａ線断面図である。 FIG. 1 is an explanatory view showing an outline of an example of a spectrophotometer of the present invention, FIG. 2 is an enlarged perspective view for explaining the main part of the spectrophotometer in FIG. 1, and FIG. 3 is a sample cell used in the present invention. FIG. 4 is a plan view of a sample cell used in the present invention, and FIG. 5 is a sectional view taken along the line aa of the sample storage chamber forming plate in FIG.
本発明の分光光度計１０における試料セル１１は、透明な材質よりなる光入射側板２７Ａおよび光出射側板２７Ｂと、光入射側板２７Ａと光出射側板２７Ｂとの間に挟まれる状態で設けられる不透明な材質よりなる試料収容室形成用板２６とから構成され、全体として略直方体の形状を有している。試料セル１１は、第１凹所２５Ａと、当該第１凹所２５Ａの幅より小さい幅の第２凹所２５Ｂとからなる測定試料を収容するための試料収容室２８を有している。第１凹所２５Ａと第２凹所２５Ｂは連通し、試料セル１１の上面に開口するように形成され、試料セル１１の長手方向に沿って設けられている。 The sample cell 11 in the spectrophotometer 10 of the present invention is an opaque provided so as to be sandwiched between a light incident side plate 27A and a light output side plate 27B made of a transparent material, and the light incident side plate 27A and the light output side plate 27B. It is comprised from the sample storage chamber formation board 26 which consists of material, and has a substantially rectangular parallelepiped shape as a whole. The sample cell 11 has a sample storage chamber 28 for storing a measurement sample including a first recess 25A and a second recess 25B having a width smaller than the width of the first recess 25A. The first recess 25 </ b> A and the second recess 25 </ b> B communicate with each other, are formed to open on the upper surface of the sample cell 11, and are provided along the longitudinal direction of the sample cell 11.
試料収容室形成用板２６は、上面に、第１凹所２５Ａを形成する第１凹部２９Ａと、第１凹部２９Ａに連続する、第２凹所２５Ｂを形成する第２凹部２９Ｂとを有している。試料収容室形成用板２６は、２つの異なる厚さ部分を有し、第１凹部２９Ａは厚さの大きい部分に形成され、第２凹部２９Ｂは厚さの小さい部分に形成されている。試料収容室形成用板２６における第１凹部２９Ａと第２凹部２９Ｂの試料セル１１の長手方向の両側面は、試料セル１１の上方に向かうに従って広がるように傾斜している。光入射側板２７Ａと光出射側板２７Ｂは、試料収容室形成用板２６の厚さ部分の大きさに応じて、試料セル１１全体が略直方体の形状となるように適合した厚さを有する構成とされている。 The sample storage chamber forming plate 26 has, on the upper surface, a first recess 29A that forms the first recess 25A, and a second recess 29B that forms a second recess 25B that is continuous with the first recess 29A. ing. The sample storage chamber forming plate 26 has two different thickness portions, the first concave portion 29A is formed in a portion having a large thickness, and the second concave portion 29B is formed in a portion having a small thickness. Both side surfaces in the longitudinal direction of the sample cell 11 of the first recess 29A and the second recess 29B in the sample storage chamber forming plate 26 are inclined so as to expand toward the upper side of the sample cell 11. The light incident side plate 27A and the light emitting side plate 27B have a configuration that has a thickness that is adapted so that the entire sample cell 11 has a substantially rectangular parallelepiped shape according to the size of the thickness portion of the sample storage chamber forming plate 26. Has been.
試料収容室形成用板２６の材質は、例えばステンレス鋼であり、光入射側板２７Ａおよび光出射側板２７Ｂの材質は、例えば石英ガラスである。 The material of the sample storage chamber forming plate 26 is, for example, stainless steel, and the material of the light incident side plate 27A and the light emitting side plate 27B is, for example, quartz glass.
試料セル１１における各部位の具体的な寸法例を示すと、第１凹所２５Ａの開口幅Ｌ1 は、１ｍｍ、第２凹所２５Ｂの開口幅Ｌ2 は、０．２ｍｍである。試料収容室２８の第１凹所２５Ａ側の長手方向の長さが１．６ｍｍ、第２凹所２５Ｂの長手方向の長さが１．６ｍｍであり、全長Ｄは３．２ｍｍである。 An example of a specific dimension of each part in the sample cell 11 is shown. The opening width L 1 of the first recess 25A is 1 mm, and the opening width L 2 of the second recess 25B is 0.2 mm. The length of the sample storage chamber 28 in the longitudinal direction on the first recess 25A side is 1.6 mm, the length of the second recess 25B in the longitudinal direction is 1.6 mm, and the total length D is 3.2 mm.
試料セル１１の具体的な寸法例は、例えば、厚さＢが４ｍｍであり、高さＣが１０ｍｍであり、長さＡが１０ｍｍである。 Specific examples of dimensions of the sample cell 11 include, for example, a thickness B of 4 mm, a height C of 10 mm, and a length A of 10 mm.
第１凹所２５Ａおよび第２凹所２５Ｂの深さｈは、０．４ｍｍ以下であることが好ましく、特に０．３ｍｍであることが好ましい。 The depth h of the first recess 25A and the second recess 25B is preferably 0.4 mm or less, and particularly preferably 0.3 mm.
分光光度計１０は、中央部に試料セル収容空間３０が形成されたファイバー可動台１９を有し、ファイバー可動台１９には、光源１２から伸びる投光ファイバー１３の一端に出射端１５ａが設けられ、試料セル収容空間３０を介して出射端１５ａと対向する位置に、受光部１６から伸びる受光ファイバー１４の一端に受光端１５ｂが設けられている。受光部１６は中央演算装置１７に接続されている。投光ファイバー１３と受光ファイバー１４は、出射端１５ａと受光端１５ｂを含む一部がファイバー可動台１９に埋設されている。ファイバー可動台１９は、プランジャー２２を介して電動ソレノイド２３と接続されている。 The spectrophotometer 10 has a fiber movable base 19 in which a sample cell accommodating space 30 is formed in the center, and the fiber movable base 19 is provided with an emission end 15 a at one end of a projecting optical fiber 13 extending from the light source 12. A light receiving end 15 b is provided at one end of the light receiving optical fiber 14 extending from the light receiving portion 16 at a position facing the emission end 15 a through the sample cell accommodating space 30. The light receiving unit 16 is connected to the central processing unit 17. The projecting optical fiber 13 and the receiving optical fiber 14 are partially embedded in the fiber movable table 19 including the emitting end 15 a and the receiving end 15 b. The fiber movable table 19 is connected to an electric solenoid 23 via a plunger 22.
出射端１５ａと受光端１５ｂは一直線上に設けられ、試料セル１１は、出射端１５ａと受光端１５ｂとに挟まれる位置において試料セル収容空間３０内に、出射端１５ａと受光端１５ｂとの一直線上に試料セル１１の長手方向が直交するように配置されている。試料セル１１はリニアベアリング２４によって支持固定されている。 The emitting end 15a and the light receiving end 15b are provided on a straight line, and the sample cell 11 is placed in a straight line between the emitting end 15a and the light receiving end 15b in the sample cell accommodating space 30 at a position sandwiched between the emitting end 15a and the light receiving end 15b. It arrange | positions so that the longitudinal direction of the sample cell 11 may orthogonally cross on a line. The sample cell 11 is supported and fixed by a linear bearing 24.
この分光光度計１０は、光路長選択機構１８を有する。この光路選択機構１８は、電動ソレノイド２３を動力源として、プランジャー２２を介してファイバー可動台１９を試料セル１１の長手方向（図１の矢印方向）に往復移動させることにより、第１凹所２５Ａをその幅方向に測定光が第１光軸２０Ａを通過する第１の光路と、第２凹所２５Ｂをその幅方向に測定光が第２光軸２０Ｂを通過する第２の光路のいずれか一方を選択する機能を有するものとされている。 The spectrophotometer 10 has an optical path length selection mechanism 18. The optical path selection mechanism 18 uses the electric solenoid 23 as a power source to reciprocate the fiber movable table 19 through the plunger 22 in the longitudinal direction of the sample cell 11 (in the direction of the arrow in FIG. 1). The first optical path through which the measurement light passes through the first optical axis 20A in the width direction of 25A and the second optical path through which the measurement light passes through the second optical axis 20B in the width direction of the second recess 25B It has a function of selecting one of them.
第１光軸２０Ａと第２光軸２０Ｂの離間距離Ｇは、例えば１．６ｍｍであり、第１光軸２０Ａおよび第２光軸２０Ｂと試料セル１１の上面までの深さｇは、例えば０．２ｍｍである。 The separation distance G between the first optical axis 20A and the second optical axis 20B is, for example, 1.6 mm, and the depth g to the upper surface of the first optical axis 20A, the second optical axis 20B, and the sample cell 11 is, for example, 0. .2 mm.
本発明の分光光度計１０によれば、測定試料の吸光度を以下のようにして測定することができる。
例えば、まず、ピペットチップ等を用いて測定試料を試料収容室２８に注入し、試料セル１１を試料セル収容空間３０にセットする。次いで、光路選択機構１８によりファイバー可動台１９を移動させ、出射端１５ａと受光端１５ｂとの間の光路位置を第１光軸２０Ａに合わせて第１の光路を選択する。第１の光路を選択した場合において、光源１２から出射された光は、投光ファイバー１３を通過して出射端１５ａから第１光軸２０Ａを通って、受光端１５ｂへ入射する。そして、受光した光が受光ファイバー１４を通過し、受光部１６および中央演算装置１７によって測定試料の吸光度が計測される。第１の光路を選択した場合は、測定光路長（第１凹所２５Ａの開口幅）Ｌ1 ＝１ｍｍで測定が行われる。
According to the spectrophotometer 10 of the present invention, the absorbance of the measurement sample can be measured as follows.
For example, first, a measurement sample is injected into the sample storage chamber 28 using a pipette tip or the like, and the sample cell 11 is set in the sample cell storage space 30. Next, the fiber movable table 19 is moved by the optical path selection mechanism 18, and the first optical path is selected by matching the optical path position between the emitting end 15a and the light receiving end 15b with the first optical axis 20A. When the first optical path is selected, the light emitted from the light source 12 passes through the projecting optical fiber 13 and enters the light receiving end 15b from the emitting end 15a through the first optical axis 20A. Then, the received light passes through the receiving optical fiber 14, and the absorbance of the measurement sample is measured by the light receiving unit 16 and the central processing unit 17. When the first optical path is selected, the measurement is performed with the measurement optical path length (the opening width of the first recess 25A) L 1 = 1 mm.
必要に応じて、第２の光路を選択することができ、光路選択機構１８によりファイバー可動台１９を移動させ、出射端１５ａと受光端１５ｂとの間の光路位置を第２光軸２０Ｂに合わせて第２の光路を選択する。その場合は測定光路長（第２凹所２５Ｂの開口幅）Ｌ2 ＝０．２ｍｍで測定が行われる。 The second optical path can be selected as necessary, and the optical fiber position 19 is moved by the optical path selection mechanism 18 so that the optical path position between the emitting end 15a and the light receiving end 15b is aligned with the second optical axis 20B. To select the second optical path. In that case, the measurement is performed with the measurement optical path length (opening width of the second recess 25B) L 2 = 0.2 mm.
本発明の分光光度計１０によれば、第１の光路の測定光路長Ｌ1 と第２の光路の測定光路長Ｌ2 のいずれか一方を任意に選択することができる。従って、同一の測定試料について適切な条件で吸光度測定を簡便に行うことができる。また、吸光度が大きく異なる複数の測定試料についても、試料セルの交換を必要とせず、同一の試料セルで測定することができる。 According to the spectrophotometer 10 of the present invention, any one of the measurement optical path length L 1 of the first optical path and the measurement optical path length L 2 of the second optical path can be arbitrarily selected. Therefore, the absorbance measurement can be easily performed on the same measurement sample under appropriate conditions. In addition, a plurality of measurement samples having greatly different absorbances can be measured in the same sample cell without replacing the sample cell.
また、本発明の分光光度計１０によれば、第１凹所２５Ａおよび第２凹所２５Ｂの深さを０．４ｍｍ以下とすること、並びに、第１凹部２９Ａと第２凹部２９Ｂの試料セル１１の長手方向の両側面が試料セル１１の上方に向かうに従って広がるように傾斜していることにより、試料収容室２８に収容された測定試料を、ティッシュペーパーや布等で拭い易く、容易に洗浄を行うことができる。特に、ティッシュペーパー等を濡らした状態で拭うとより洗浄効果が向上する。 Further, according to the spectrophotometer 10 of the present invention, the depth of the first recess 25A and the second recess 25B is 0.4 mm or less, and the sample cell of the first recess 29A and the second recess 29B. 11 is inclined so that both side surfaces in the longitudinal direction expand toward the upper side of the sample cell 11, so that the measurement sample accommodated in the sample accommodating chamber 28 can be easily wiped with tissue paper or cloth, and easily washed. It can be performed. In particular, when the tissue paper is wiped in a wet state, the cleaning effect is further improved.
図６および図７に示すように、本発明に係る試料セル１１においては、その上面をマスク層３１で覆う構成とすることができる。このマスク層３１は、試料セル１１上面の第１凹所２５Ａおよび第２凹所２５Ｂの開口の周縁部領域を覆う構成とされる。 As shown in FIGS. 6 and 7, the sample cell 11 according to the present invention can be configured so that the upper surface thereof is covered with a mask layer 31. This mask layer 31 is configured to cover the peripheral region of the opening of the first recess 25A and the second recess 25B on the upper surface of the sample cell 11.
マスク層３１は、疎水性の材質のものが好ましく、さらに不透明な材質のものが好ましい。例えば、プラスティック、金属等が挙げられる。マスク層３１の材質が、疎水性のものであることにより、仮に測定試料２１が試料収容室２８から外れた場合においても、マスク層３１と測定試料２１との間の界面張力が小さいために、測定試料２１が試料収容室２８の外方へ流れ出ることを防ぐことができる。また、マスク層３１が不透明な材質のものであることにより、ピペットチップ等で測定試料２１を注入する際に、先端の位置を確定し易くすることができる。
マスク層３１の厚さは、０．１ｍｍ以下が好ましい。
The mask layer 31 is preferably made of a hydrophobic material, and more preferably made of an opaque material. For example, a plastic, a metal, etc. are mentioned. Since the material of the mask layer 31 is hydrophobic, even when the measurement sample 21 is removed from the sample storage chamber 28, the interfacial tension between the mask layer 31 and the measurement sample 21 is small. It is possible to prevent the measurement sample 21 from flowing out of the sample storage chamber 28. Further, since the mask layer 31 is made of an opaque material, the position of the tip can be easily determined when the measurement sample 21 is injected with a pipette tip or the like.
The thickness of the mask layer 31 is preferably 0.1 mm or less.
図８に示すように、本発明に係る試料セル１１においては、カバー体３２を設ける構成とすることができる。このカバー体３２は、第１凹所２５Ａおよび第２凹所２５Ｂの開口を覆い、開閉可能な構成とされる。カバー体３２には、凸部３３が具えられており、凸部３３は、第１凹所２５Ａおよび第２凹所２５Ｂの開口の周縁部領域を覆うよう設けられたマスク層３１の開口部形状に適合した角柱の形状でカバー体３２に設けられている。 As shown in FIG. 8, in the sample cell 11 according to the present invention, a cover body 32 can be provided. The cover body 32 covers the openings of the first recess 25A and the second recess 25B and is configured to be openable and closable. The cover body 32 is provided with a convex portion 33, and the convex portion 33 has an opening shape of the mask layer 31 provided so as to cover the peripheral region of the opening of the first recess 25A and the second recess 25B. The cover body 32 is provided with a prismatic shape suitable for the above.
本発明の分光光度計１０によれば、凸部３３を具えるカバー体３２を有することにより、第１凹所２５Ａおよび第２凹所２５Ｂの開口を覆うので、測定試料の蒸発を防ぐことができる。
さらに、図８に示すように、測定試料２１が流動性の低いものである場合において、第２凹所２５Ｂのような幅の狭い試料収容室２８に測定試料２１を完全に充填することが困難な場合がある。このような場合において、凸部３３を具えるカバー体３２を用いることにより、上から測定試料２１を押し込むようにして、測定試料２１を試料収容室２８全体に完全に充填することができる。
According to the spectrophotometer 10 of the present invention, by having the cover body 32 having the convex portion 33, the openings of the first recess 25A and the second recess 25B are covered, so that evaporation of the measurement sample can be prevented. it can.
Furthermore, as shown in FIG. 8, when the measurement sample 21 has low fluidity, it is difficult to completely fill the measurement sample 21 into the narrow sample storage chamber 28 such as the second recess 25B. There is a case. In such a case, by using the cover body 32 having the convex portion 33, the measurement sample 21 can be completely filled in the entire sample storage chamber 28 by pushing the measurement sample 21 from above.
上記構成の分光光度計１０によれば、幅の大きさが異なる２つ凹所２５Ａ，２５Ｂよりなる試料収容室２８を有する試料セル１１と、光路を選択する光路選択機構１８とを有することにより、測定光路長の異なる光路を任意に選択することができるので、同一の測定試料について適切な条件で吸光度測定を簡便に行うことができる。従って、吸光度が大きく異なる複数の測定試料についても、試料セルの交換を必要とせず、同一の試料セル１１で測定することができる。
また、本発明の分光光度計１０によれば、試料セル１１において、試料収容室２８の凹所２５Ａ，２５Ｂの深さを小さい構成とすることにより、ティッシュペーパーや布等で測定試料を簡単に拭き取ることができ、試料収容室２８の洗浄を容易に行うことができる。
さらに、本発明の分光光度計１０によれば、疎水性の材質よりなるマスク層３１が試料セル１１の上面を覆う構成により、極微量の測定試料２１を試料収容室２８へ注入するときに、仮に測定試料２１が試料収容室２８から外れた場合においても、測定試料２１が試料収容室２８の外方へ流れ出ることを防ぐことができる。
さらにまた、本発明の分光光度計１０によれば、第１凹所２５Ａおよび第２凹所２５Ｂの開口を覆うためのカバー体３２を有することにより、測定試料２１の蒸発を防ぐことができる。
According to the spectrophotometer 10 having the above-described configuration, by including the sample cell 11 having the sample storage chamber 28 composed of the two recesses 25A and 25B having different widths, and the optical path selection mechanism 18 that selects the optical path. Since optical paths having different measurement optical path lengths can be arbitrarily selected, it is possible to easily perform absorbance measurement under appropriate conditions for the same measurement sample. Therefore, a plurality of measurement samples having greatly different absorbances can be measured with the same sample cell 11 without requiring replacement of the sample cell.
Further, according to the spectrophotometer 10 of the present invention, in the sample cell 11, the depth of the recesses 25 </ b> A and 25 </ b> B of the sample storage chamber 28 is made small so that the measurement sample can be easily made with tissue paper or cloth. The sample storage chamber 28 can be easily cleaned.
Furthermore, according to the spectrophotometer 10 of the present invention, when the mask layer 31 made of a hydrophobic material covers the upper surface of the sample cell 11, when a very small amount of the measurement sample 21 is injected into the sample storage chamber 28, Even if the measurement sample 21 is removed from the sample storage chamber 28, the measurement sample 21 can be prevented from flowing out of the sample storage chamber 28.
Furthermore, according to the spectrophotometer 10 of the present invention, it is possible to prevent evaporation of the measurement sample 21 by having the cover body 32 for covering the openings of the first recess 25A and the second recess 25B.
以上、本発明の実施形態について説明したが、本発明は上記の実施形態に限定されるものではなく、種々の変更を加えることができる。
例えば、本発明の分光光度計においては、光路選択機構と、幅の異なる複数の凹所を有する試料セルとを有する構成とされていれば、その具体的な構成、例えば凹所の数、凹所の形状等は、特に限定されるものではなく、適宜変更することができる。
また、光路選択機構も特に限定されるものではなく、試料セルを移動させて光路長を選択する構成としてもよい。
As mentioned above, although embodiment of this invention was described, this invention is not limited to said embodiment, A various change can be added.
For example, in the spectrophotometer of the present invention, if the optical path selection mechanism and the sample cell having a plurality of recesses having different widths are used, the specific configuration thereof, for example, the number of recesses, The shape of the place is not particularly limited and can be changed as appropriate.
Further, the optical path selection mechanism is not particularly limited, and the optical path length may be selected by moving the sample cell.
１０ 分光光度計
１１ 試料セル
１２ 光源
１３ 投光ファイバー
１４ 受光ファイバー
１５ａ 出射端
１５ｂ 受光端
１６ 受光部
１７ 中央演算装置
１８ 光路選択機構
１９ ファイバー可動台
２０Ａ 第１光軸
２０Ｂ 第２光軸
２１ 測定試料
２２ プランジャー
２３ 電動ソレノイド
２４ リニアベアリング
２５Ａ 第１凹所
２５Ｂ 第２凹所
２６ 試料収容室形成用板
２７Ａ 光入射側板
２７Ｂ 光出射側板
２８ 試料収容室
２９Ａ 第１凹部
２９Ｂ 第２凹部
３０ 試料セル収容空間
３１ マスク層
３２ カバー体
３３ 凸部
DESCRIPTION OF SYMBOLS 10 Spectrophotometer 11 Sample cell 12 Light source 13 Throwing optical fiber 14 Receiving optical fiber 15a Outgoing end 15b Receiving end 16 Light receiving part 17 Central processing unit 18 Optical path selection mechanism 19 Fiber movable stand 20A 1st optical axis 20B 2nd optical axis 21 Measurement sample 22 Plunger 23 Electric solenoid 24 Linear bearing 25A First recess 25B Second recess 26 Sample storage chamber forming plate 27A Light incident side plate 27B Light output side plate 28 Sample storage chamber 29A First recess 29B Second recess 30 Sample cell storage space 31 Mask layer 32 Cover body 33 Projection
Claims (5)
第１凹所をその幅方向に測定光が通過する第１の光路と、第２凹所をその幅方向に測定光が通過する第２の光路のいずれか一方を選択する光路選択機構とを有することを特徴とする分光光度計。 A sample cell having a sample storage chamber comprising a first recess and a second recess communicating with the first recess and having a width different from that of the first recess;
A first optical path through which the measurement light passes through the first recess in the width direction; and an optical path selection mechanism for selecting one of the second optical paths through which the measurement light passes through the second recess in the width direction. A spectrophotometer characterized by comprising:
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55125527U (en) * | 1979-02-28 | 1980-09-05 | ||
| JPH04181146A (en) * | 1990-11-15 | 1992-06-29 | Sumitomo Electric Ind Ltd | Fluid detector using optical fiber |
| JPH08313429A (en) * | 1995-05-17 | 1996-11-29 | Hitachi Ltd | Cell for spectrophotometer |
| JPH11248622A (en) * | 1998-02-27 | 1999-09-17 | Sekisui Chem Co Ltd | Urinalysis device |
| JP2001041879A (en) * | 1999-08-02 | 2001-02-16 | Yokogawa Electric Corp | Spectral analyzing vial bottle |
| JP2006112789A (en) * | 2004-10-12 | 2006-04-27 | Sekisui Chem Co Ltd | Microanalysis method |
| JP2007271560A (en) * | 2006-03-31 | 2007-10-18 | Shimadzu Corp | Spectrophotometer |
-
2008
- 2008-10-21 JP JP2008270700A patent/JP2010101634A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55125527U (en) * | 1979-02-28 | 1980-09-05 | ||
| JPH04181146A (en) * | 1990-11-15 | 1992-06-29 | Sumitomo Electric Ind Ltd | Fluid detector using optical fiber |
| JPH08313429A (en) * | 1995-05-17 | 1996-11-29 | Hitachi Ltd | Cell for spectrophotometer |
| JPH11248622A (en) * | 1998-02-27 | 1999-09-17 | Sekisui Chem Co Ltd | Urinalysis device |
| JP2001041879A (en) * | 1999-08-02 | 2001-02-16 | Yokogawa Electric Corp | Spectral analyzing vial bottle |
| JP2006112789A (en) * | 2004-10-12 | 2006-04-27 | Sekisui Chem Co Ltd | Microanalysis method |
| JP2007271560A (en) * | 2006-03-31 | 2007-10-18 | Shimadzu Corp | Spectrophotometer |
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