JP2009079016A - Raw material for cosmetic and cosmetic - Google Patents
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本発明は、８−ヒドロキシ−２’−デオキシグアノシン形成抑制能およびコラーゲン合成促進能を有する化粧料用原料およびこの化粧料用原料を配合した肌荒れ改善効果の非常に高い化粧料に関する。 The present invention relates to a cosmetic raw material having the ability to suppress the formation of 8-hydroxy-2'-deoxyguanosine and the ability to promote collagen synthesis, and a cosmetic having a very high effect of improving skin roughness by blending this cosmetic raw material.
従来、皮膚外用剤にプロテオグリカンが利用されてきた。例えば、プロテオグリカン抽出液にＬ−アスコルビン酸リン酸塩を配合した美白化粧料が知られている（特許文献１）。また、皮膚外用剤に用いられるプロテオグリカンとして、サメ軟骨由来のプロテオグリカンを得る方法が知られている（特許文献２）。従来使用されていた牛、豚等の高等動物由来のプロテオグリカンと比べ、サメ軟骨由来のプロテオグリカンは透明性が高く、皮膚外用剤の原料として有効であり、シミ、皺、タルミの改善を目的として、このサメ軟骨由来プロテオグリカンにメラニン生成抑制剤や活性酸素消去作用を有する生薬抽出物を組み合わせることが知られている（特許文献３）。
また、酸化防止剤として、サレン−金属錯体を用いることが知られている（特許文献４）。
Conventionally, proteoglycan has been used as an external preparation for skin. For example, a whitening cosmetic is known in which L-ascorbic acid phosphate is blended in a proteoglycan extract (Patent Document 1). Further, as a proteoglycan used in a skin external preparation, a method for obtaining shark cartilage-derived proteoglycan is known (Patent Document 2). Compared to the proteoglycans derived from higher animals such as cattle and pigs that have been used in the past, shark cartilage-derived proteoglycans are highly transparent and effective as a raw material for external preparations for skin, wrinkles, and tarmi. It is known that this shark cartilage-derived proteoglycan is combined with a melanin production inhibitor and a herbal extract having an active oxygen scavenging action (Patent Document 3).
In addition, it is known to use a salen-metal complex as an antioxidant (Patent Document 4).
皮膚外用剤、特に化粧料において求められる効果は、基本的には実際の年齢より若くみせたいという要求を叶えることである。年齢が高く見える理由には、シミもあるが、皺やタルミが占める割合が高い。皺やタルミの原因として、活性酸素の作用によりＤＮＡ中のグアニン塩基が酸化損傷を受けることによる８−ヒドロキシ−２’−デオキシグアノシン（以下、「８−ＯＨｄＧ」という）の形成や、細胞外マトリックス成分であるコラーゲンの減少などが挙げられる。
しかし、８−ＯＨｄＧ形成を抑制すると共にコラーゲン合成を促進することができる化粧料用原料は知られておらず、また、この化粧料用原料を用いて、皺やタルミを減少させて肌荒れ改善効果に優れた化粧料は知られていない。
However, there is no known cosmetic raw material that can suppress 8-OHdG formation and promote collagen synthesis, and this cosmetic raw material can be used to reduce wrinkles and tarmi, thereby improving skin roughness. No good cosmetics are known.
本発明はこのような問題に対処するためになされたもので、特に肌の皺やタルミなどの改善を目的として、８−ＯＨｄＧ形成抑制能およびコラーゲン合成促進能を有する化粧料用原料の提供、およびこの原料を用いることにより肌荒れ改善効果に優れた化粧料の提供を目的とする。 The present invention was made to cope with such problems, and in particular, for the purpose of improving skin wrinkles and talmi, providing a cosmetic raw material having an ability to suppress 8-OHdG formation and an ability to promote collagen synthesis, And it aims at provision of the cosmetics excellent in the rough skin improvement effect by using this raw material.
本発明の化粧料用原料は、サメ軟骨より抽出したプロテオグリカンとエチルビスイミノメチルグアヤコールマンガンクロリドとを含み、８−ＯＨｄＧ形成抑制能およびコラーゲン合成促進能を有することを特徴とする。
また、さらに１種以上のアミノ酸を配合することを特徴とする。上記アミノ酸が、グリシン、グルタミン酸、およびアルギニンから選ばれた少なくとも１つのアミノ酸であることを特徴とする。
The raw material for cosmetics of the present invention contains proteoglycan extracted from shark cartilage and ethylbisiminomethyl guaiacol manganese chloride, and has the ability to suppress 8-OHdG formation and promote collagen synthesis.
Furthermore, it is characterized by further blending one or more amino acids. The amino acid is at least one amino acid selected from glycine, glutamic acid, and arginine.
本発明の化粧料は、上記８−ＯＨｄＧ形成抑制能およびコラーゲン合成促進能を有する化粧料用原料を配合することを特徴とする。 The cosmetic of the present invention is characterized by blending a cosmetic raw material having the above-mentioned 8-OHdG formation inhibitory ability and collagen synthesis promoting ability.
本発明の化粧料用原料は、サメ軟骨より抽出したプロテオグリカンと、サレン金属錯体であるエチルビスイミノメチルグアヤコールマンガンクロリドとを含む。これら２成分を合わせることにより、抗酸化成分である後者の活性酸素を無害化する作用と、前者のマトリックスメタロプロテアーゼ活性を阻害する作用が相乗効果をもたらし、それぞれ単独に用いたものと比較して、優れた８−ＯＨｄＧ形成抑制能を有すると共にコラーゲン合成促進能を有する化粧料用原料が得られる。さらにアミノ酸を配合するので、８−ＯＨｄＧ形成抑制能およびコラーゲン合成促進能が更に向上する。
その結果、この化粧料用原料を配合した化粧料は肌荒れ改善効果が非常に高く、皺に対して非常に有効性が高い。
The cosmetic raw material of the present invention contains proteoglycan extracted from shark cartilage and ethyl biiminomethyl guaiacol manganese chloride, which is a salen metal complex. By combining these two components, the action of detoxifying the latter active oxygen, which is an antioxidant component, and the action of inhibiting the former matrix metalloprotease activity brought about a synergistic effect, compared to those used alone. Thus, a cosmetic raw material having excellent 8-OHdG formation inhibiting ability and collagen synthesis promoting ability is obtained. Furthermore, since an amino acid is blended, the ability to suppress 8-OHdG formation and the ability to promote collagen synthesis are further improved.
As a result, a cosmetic containing this cosmetic raw material has a very high effect on improving rough skin and is very effective against wrinkles.
上記課題に対して研究の結果、サメ軟骨より抽出したプロテオグリカンとエチルビスイミノメチルグアヤコールマンガンクロリドを配合することで問題を解決できた。
プロテオグリカンは蛋白質コアに 100〜200 本のムコ多糖が結合した物質であり、軟骨、皮膚などの結合組織中に存在し、これらの組織構造を維持するために必要な物質である。皮膚外用剤には牛、豚より得られたプロテオグリカンも利用されているが、サメの軟骨より得られたプロテオグリカンの方がマトリックスメタロプロテアーゼ活性を阻害する効果が高かった。本発明の化粧料用原料に使用できるサメの種類に限定はなく、軟骨魚綱に属するサメであればよいが、実際には漁獲高が多く、他の用途にも利用され、漁法が確立しているヨシキリザメ、ネズミザメ、アオザメ等を利用することが多い。これらのサメはその鰭の部分に軟骨が多く、抽出も容易であるが、他のサメや他の部位を用いても問題はない。サメ軟骨からのプロテオグリカンの抽出は常法に従って実施することができる。なお、抽出により得られるプロテオグリカンの純度は特に問題にならないので用途や用法によってその程度を選択することができる。
As a result of research on the above problems, the problem could be solved by adding proteoglycan extracted from shark cartilage and ethylbisiminomethyl guaiacol manganese chloride.
Proteoglycan is a substance in which 100 to 200 mucopolysaccharides are bound to a protein core, is present in connective tissues such as cartilage and skin, and is a substance necessary for maintaining these tissue structures. Proteoglycans obtained from cattle and pigs are also used as topical skin preparations, but proteoglycans obtained from shark cartilage were more effective in inhibiting matrix metalloprotease activity. The type of shark that can be used in the cosmetic raw material of the present invention is not limited, and any shark belonging to the cartilage fish class may be used. Often used are blue sharks, mouse sharks, and gray sharks. These sharks have many cartilage in their wings and can be easily extracted, but there is no problem using other sharks or other parts. Extraction of proteoglycan from shark cartilage can be performed according to a conventional method. In addition, since the purity of proteoglycan obtained by extraction is not particularly problematic, the degree can be selected according to the use and usage.
サメ軟骨よりプロテオグリカンを得る方法を例示すれば、サメの鰭を細断し、水、水と低分子アルコール、塩化ナトリウム、塩化マグネシウム、塩酸グアニジン等の塩の１種または混合物を加えた液を用いて抽出する。
さらに、必要に応じて濃縮、脱塩、脱色、溶媒除去、乾燥（噴霧、凍結）、分子量分画、タンパク分解酵素処理等を行なう。
また、サメ軟骨のプロテオグリカンは化粧品原料として市販されているものを用いることができる。
サメ軟骨より抽出したプロテオグリカンの配合量は用途等によって変化するが、本発明の化粧料用原料全量に対して、固形分として 0.0001〜10 重量％、好ましくは 0.001〜5 重量％配合する。
To illustrate how to obtain proteoglycan from shark cartilage, use a solution obtained by chopping shark wrinkles and adding one or a mixture of water, water and low-molecular alcohol, sodium chloride, magnesium chloride, guanidine hydrochloride and other salts. To extract.
Furthermore, concentration, desalting, decolorization, solvent removal, drying (spraying, freezing), molecular weight fractionation, proteolytic enzyme treatment, etc. are performed as necessary.
As shark cartilage proteoglycans, those commercially available as cosmetic raw materials can be used.
The amount of proteoglycan extracted from shark cartilage varies depending on the application and the like, but is 0.0001 to 10% by weight, preferably 0.001 to 5% by weight as the solid content with respect to the total amount of the cosmetic raw material of the present invention.
サメ軟骨より抽出したプロテオグリカンに加えて、エチルビスイミノメチルグアヤコールマンガンクロリドを配合する。
エチルビスイミノメチルグアヤコールマンガンクロリドは、化学名 Chloro[[2,2'-[1,2-ethanediylbis[(nitrilo-κＮ)methylidyne]]bis[6-methoxyphenolato-κＮ]]]-manganese で、市販品としては、ＡＴＲＩＵＭ社製 商品名 ＥＵＫ−１３４などを利用できる。
エチルビスイミノメチルグアヤコールマンガンクロリドの配合量は、本発明の化粧料用原料全量に対し、0.00001〜1.0 重量％、好ましくは 0.0001〜0.1 重量％ 配合する。
エチルビスイミノメチルグアヤコールマンガンクロリドは、スーパーオキシドジスムターゼやカタラーゼのような働きがあり、傷害性の高い活性酸素や中間生成物でもある過酸化水素を生体に無害な水と酸素に変換することができ、非常に強い活性酸素消去効果が得られる。また、自己再生特性機能を有し、上記効果を持続して得ることができる。さらには広義の活性酸素である一酸化窒素等の消去作用も有する。
エチルビスイミノメチルグアヤコールマンガンクロリドにサメ軟骨より抽出したプロテオグリカンを加えると、エチルビスイミノメチルグアヤコールマンガンクロリドがもつ遺伝子損傷抑制効果（８−ＯＨｄＧ形成抑制）およびコラーゲン合成促進作用が相乗的に強化された。
In addition to proteoglycan extracted from shark cartilage, ethylbisiminomethyl guaiacol manganese chloride is added.
Ethyl bisiminomethyl guaiacol manganese chloride is commercially available under the chemical name Chloro [[2,2 '-[1,2-ethanediylbis [(nitrilo-κN) methylidyne]] bis [6-methoxyphenolato-κN]]]-manganese. For example, trade name EUK-134 manufactured by ATRIUM can be used.
The compounding amount of ethylbisiminomethyl guaiacol manganese chloride is 0.00001 to 1.0% by weight, preferably 0.0001 to 0.1% by weight, based on the total amount of the cosmetic raw material of the present invention.
Ethylbisiminomethyl guaiacol manganese chloride works like superoxide dismutase and catalase, and can convert highly toxic active oxygen and hydrogen peroxide, which is an intermediate product, into water and oxygen that are harmless to the living body. A very strong active oxygen scavenging effect can be obtained. Moreover, it has a self-regenerative characteristic function and can continuously obtain the above effects. Furthermore, it has an erasing action such as nitric oxide which is active oxygen in a broad sense.
Addition of proteoglycan extracted from shark cartilage to ethyl bisiminomethyl guaiacol manganese chloride synergistically enhanced the effect of ethyl bisiminomethyl guaiacol manganese chloride on inhibition of gene damage (suppression of 8-OHdG formation) and the promotion of collagen synthesis. .
さらに、アミノ酸を配合するとより効果を発揮する。アミノ酸としては、グリシン、アラニン、バリン、ロイシン、イソロイシン、セリン、トレオニン、システイン、メチオニン、プロリン、フェニルアラニン、トリプトファン、チロシンのような中性アミノ酸、グルタミン酸、アスパラギン酸のような酸性アミノ酸、リシン、アルギニン、ヒスチジンのような塩基性アミノ酸が挙げられる。これらの中でグリシン、グルタミン酸、およびアルギニンが好ましい。本発明の化粧料用原料では、これらの１種以上を配合する。本発明者らが検討した結果、複数の種類のアミノ酸を混合して用いると効果が高くなることが判明した。特に、中性アミノ酸、酸性アミノ酸、塩基性アミノ酸のそれぞれ１種以上を配合すると非常に効果的なことがわかった。また、大豆やコラーゲン等の加水分解物のようにアミノ酸が混合させた状態の原料を用いることもできる。 Furthermore, when an amino acid is blended, it is more effective. As amino acids, neutral amino acids such as glycine, alanine, valine, leucine, isoleucine, serine, threonine, cysteine, methionine, proline, phenylalanine, tryptophan, tyrosine, acidic amino acids such as glutamic acid, aspartic acid, lysine, arginine, Examples include basic amino acids such as histidine. Of these, glycine, glutamic acid, and arginine are preferred. In the cosmetic raw material of the present invention, one or more of these are blended. As a result of studies by the present inventors, it has been found that the effect is enhanced when a plurality of types of amino acids are used in combination. In particular, it has been found that it is very effective to add one or more of neutral amino acids, acidic amino acids, and basic amino acids. In addition, a raw material in which amino acids are mixed, such as a hydrolyzate such as soybean or collagen, can also be used.
サメ軟骨より抽出したプロテオグリカンは、マトリックスメタロプロテアーゼ（ＭＭＰ）のうち、ＭＭＰ−２，ＭＭＰ−９の活性を阻害する。これらの酵素はVII型コラーゲンやエラスチン等の分解に関与しており、皮膚の細胞外マトリックスを萎縮させる酵素である。これは動物性コラーゲナーゼと呼ばれるが、このほかにも細菌性コラーゲナーゼが存在し、これらに対してアミノ酸が有効であることが知られている。また、サメ軟骨より抽出したプロテオグリカンとアミノ酸を組み合わせることにより、多くの種類のマトリックスメタロプロテアーゼの作用を抑制し、皮膚のマトリックスであるコラーゲンの合成を促進して、コラーゲンの維持や強化を行ない、皺の予防、減少、タルミの防止等を効果的に行なうことができる。
エチルビスイミノメチルグアヤコールマンガンクロリドおよびサメ軟骨より抽出したプロテオグリカンに、さらにアミノ酸を配合することによって、８−ＯＨｄＧ形成抑制能、および特にコラーゲン合成促進作用が相乗的に効果を増すことが本発明者によって見出された。
Proteoglycan extracted from shark cartilage inhibits the activities of MMP-2 and MMP-9 among matrix metalloproteases (MMP). These enzymes are involved in the degradation of type VII collagen and elastin, and are enzymes that atrophy the extracellular matrix of the skin. This is called animal collagenase, but there are other bacterial collagenases, and amino acids are known to be effective against these. In addition, combining proteoglycan extracted from shark cartilage and amino acids suppresses the action of many types of matrix metalloproteases, promotes the synthesis of collagen, the skin matrix, maintains and strengthens collagen, It is possible to effectively prevent, reduce and prevent tarmi.
According to the present inventor, by adding an amino acid to proteoglycan extracted from ethylbisiminomethyl guaiacol manganese chloride and shark cartilage, the ability to suppress 8-OHdG formation, and particularly the action of promoting collagen synthesis, synergistically increases the effect. It was found.
アミノ酸の配合量は、用途、用法によって大きく異なるので、一概には示すことはできないが、本発明の化粧料原料全量に対し、0.1〜5 重量％程度配合されることが好ましい。 The amount of amino acid varies greatly depending on the application and usage, and cannot be generally shown, but it is preferably about 0.1 to 5% by weight based on the total amount of the cosmetic raw material of the present invention.
上述した化粧料用原料のほかに必要な原料を加えて、化粧料を作製する。その中で、他の有効性物質、例えば、アスコルビン酸およびその誘導体、ビタミンＡおよびその誘導体、類似体、ヒアルロン酸およびその塩、各種植物抽出物等を併用することは本発明の付加価値を高めるので配合することがより好ましい。
また本発明の化粧料は、用途等によって、クリーム、乳液、ローション、パック、スプレー、ジェル等任意の剤型より選択することは、なんら問題はない。また、医薬品、医薬部外品、化粧品のいずれでもよく、皮膚に適用するものであれば、入浴剤、ファンデーション等の形態をとってもよい。
In addition to the cosmetic raw materials described above, the necessary raw materials are added to produce cosmetics. Among them, the combined use of other effective substances such as ascorbic acid and its derivatives, vitamin A and its derivatives, analogs, hyaluronic acid and its salts, various plant extracts, etc. increases the added value of the present invention. Therefore, it is more preferable to mix them.
Moreover, there is no problem in selecting the cosmetics of the present invention from arbitrary dosage forms such as creams, emulsions, lotions, packs, sprays, gels, etc. depending on applications. Moreover, any of pharmaceuticals, quasi-drugs, and cosmetics may be used. As long as they are applied to the skin, forms such as bathing agents and foundations may be used.
以下に実施例を記載するが、当然これに限定させるものではない。なお、以下表中の配合量は重量部で表した。 Although an Example is described below, naturally it is not limited to this. In addition, the compounding quantity in the following table | surface was represented by the weight part.
実施例１〜実施例４および比較例１〜比較例４
表１および表２に示す原料を、表１および表２に示す配合割合で攪拌混合し、化粧料用原料を得た。なお、サメ軟骨抽出液はＡＥＴＥＲＮＡ社製 商品名 ＭＤＩＣＯＭＰＬＥＸ（有効成分含量： 0.4 重量％）を用いた。また、エチルビスイミノメチルグアヤコールマンガンクロリドとして、ＡＴＲＩＵＭ社製 商品名 ＥＵＫ−１３４（有効成分含量： 95 重量％以上）を用いた。
得られた化粧料用原料を用いて、以下に示す８−ＯＨｄＧ形成抑制試験およびコラーゲン合成促進試験を行なった。
Examples 1 to 4 and Comparative Examples 1 to 4
The raw materials shown in Tables 1 and 2 were stirred and mixed at the blending ratios shown in Tables 1 and 2 to obtain cosmetic raw materials. The shark cartilage extract used was trade name MDICOMPLEX (active ingredient content: 0.4% by weight) manufactured by AETERA. As ethylbisiminomethyl guaiacol manganese chloride, trade name EUK-134 (active ingredient content: 95% by weight or more) manufactured by ATRIUM was used.
Using the obtained cosmetic raw material, the following 8-OHdG formation inhibition test and collagen synthesis promotion test were conducted.
試験１ ８−ＯＨｄＧ形成抑制試験
ヒト由来のケラチノサイト培養細胞を使用し、上述の実施例１〜４および比較例１〜４の化粧料用原料を用いて、８−ＯＨｄＧ形成抑制効果を調べた。
各実施例および比較例の化粧料用原料の最終濃度が 0.01 ％または 0.1 ％になるようにした培地で４日間培養した。その後、それぞれ 0 mJ/cm2（未照射）、50 mJ/cm2、100 mJ/cm2 の紫外線を照射し、ケラチノサイトからＤＮＡを抽出し、イムノドットブロット法を用いて、８−ＯＨｄＧ形成率を測定した。
Test 1 8-OHdG formation inhibition test Using human-derived keratinocyte cultured cells, the 8-OHdG formation inhibition effect was examined using the cosmetic raw materials of Examples 1 to 4 and Comparative Examples 1 to 4 described above.
Each of the examples and comparative examples was cultured for 4 days in a medium in which the final concentration of the cosmetic raw material was 0.01% or 0.1%. Thereafter, ultraviolet rays of 0 mJ / cm 2 (unirradiated), 50 mJ / cm 2 and 100 mJ / cm 2 were irradiated to extract DNA from keratinocytes, and 8-OHdG formation rate using immunodot blotting Was measured.
また、同様に育成した培養細胞をホルマリン固定後、1 ％クリスタルバイオレット溶液に添加し染色した。各化粧料用原料の各濃度に対する細胞育成率を、モノセレーター（オリンパス社製）で測定した。
これらの結果を表３〜表１０に示す。表中、数値は８−ＯＨｄＧ形成率を、また括弧内の数値は細胞育成率を示し、いずれも実施例（または比較例）を含まない紫外線量が0 mJ/cm2（未照射）のときを１として表示した。
Similarly, cultured cells grown in the same manner were fixed in formalin, added to a 1% crystal violet solution and stained. The cell growth rate for each concentration of each cosmetic raw material was measured with a monocerator (manufactured by Olympus).
These results are shown in Tables 3 to 10. In the table, the numerical value indicates the 8-OHdG formation rate, and the numerical value in parentheses indicates the cell growth rate. When the amount of ultraviolet rays does not include any example (or comparative example) is 0 mJ / cm 2 (unirradiated) Was displayed as 1.
実施例１〜４の化粧料用原料を用いた培地で培養した細胞では比較例を用いた細胞に比べて、紫外線量を増大しても８−ＯＨｄＧ形成が行なわれにくく、優れた８−ＯＨｄＧ形成抑制効果がみられた。また、実施例１〜４を用いた培地で培養したものは比較例を用いたものに比べて、紫外線量を増大しても細胞育成率の低下率が低く、細胞育成能の劣化についても抑制効果がみられた。 In cells cultured in a medium using the cosmetic raw materials of Examples 1 to 4, 8-OHdG formation is less likely to occur even when the amount of ultraviolet rays is increased, compared to cells using Comparative Examples, and excellent 8-OHdG A formation inhibitory effect was observed. Moreover, compared with the thing using the comparative example, even if it increased the amount of ultraviolet rays, the fall rate of the cell growth rate was low and what was cultured with the culture medium using Examples 1-4 also suppressed deterioration of cell growth ability. The effect was seen.
試験２ コラーゲン合成促進試験
ヒト皮膚線維芽細胞（ＡＴＣＣ ＣＣＬ １１０）を用い、９６穴プレートにヒト皮膚線維芽細胞を 5000 cells／well 播種し、10 ％ウシ胎仔血清を含むＥａｇｌｅ'ｓ ＭＥＭ培地にて、37 ℃、5 ％ ＣＯ2条件下でコンフルエントの状態になるまで４日間培養した。これに実施例１〜４および比較例１〜４を、培養濃度を 1.0 ％となるように調整した無血清のＥａｇｌｅ'ｓ ＭＥＭ（ 50 μg／ml Ｌ−アスコルビン酸、50 μg／ml β−アミノプロピオニトリル、18.5 KBq ３Ｈ−プロリン含有）に交換し、２４時間培養した。その後、直ちに各 well にペプシン溶液を加えインキュベーションした後、３Ｈ−プロリン標識されたコラーゲンを抽出した。塩析により精製した後、コラーゲンタンパク中の放射活性を液体シンチレーションカウンターにより測定した。各試料とも 5 well ずつ測定し、試料無添加のものをコントロールとして用い、これを１としてコラーゲン合成促進倍率を表した。結果を表１１に示す。
Test 2 Collagen synthesis promotion test Human skin fibroblasts (ATCC CCL 110) were used to seed 5000 cells / well of human skin fibroblasts in a 96-well plate, and in Eagle's MEM medium containing 10% fetal bovine serum. The cells were cultured for 4 days at 37 ° C. under 5% CO 2 until they became confluent. To this, Examples 1 to 4 and Comparative Examples 1 to 4 were serum-free Eagle's MEM (50 μg / ml L-ascorbic acid, 50 μg / ml β-amino) adjusted to a culture concentration of 1.0%. The mixture was replaced with propionitrile (containing 18.5 KBq 3H-proline) and cultured for 24 hours. Then, immediately after adding a pepsin solution to each well and incubating, 3H-proline labeled collagen was extracted. After purification by salting out, the radioactivity in the collagen protein was measured with a liquid scintillation counter. Each sample was measured in 5 wells, and the sample-free sample was used as a control. The results are shown in Table 11.
実施例１〜４では、比較例に比べ、コラーゲン合成促進倍率において良好な結果を得た。特に、アミノ酸であるグリシン、グルタミン酸およびアルギニンの３種全てを使用した実施例４では、優れたコラーゲン合成促進能を有していた。 In Examples 1 to 4, good results were obtained in the collagen synthesis promotion magnification as compared with Comparative Examples. In particular, Example 4 using all three amino acids glycine, glutamic acid, and arginine had excellent ability to promote collagen synthesis.
実施例５〜実施例８および比較例５〜比較例６
表１２および表１３に示す配合で、Ａ成分とＢ成分とをそれぞれ計量し、それぞれを 70 ℃まで加温して、Ｂ成分にＡ成分を攪拌しつつ徐々に加えたのち、ゆっくり攪拌しつつ 30 ℃まで冷却して化粧料を得た。
得られた化粧料を用いて、以下に示す肌荒れ改善試験を行なった。
Examples 5 to 8 and Comparative Examples 5 to 6
In the formulations shown in Table 12 and Table 13, the A component and the B component were weighed, each was heated to 70 ° C., and the A component was gradually added to the B component while stirring, and then slowly stirred. A cosmetic was obtained by cooling to 30 ° C.
Using the resulting cosmetic, the following rough skin improvement test was conducted.
試験３ 肌荒れ改善試験
ハイドロフィリック エクザフレックス親水性ビニルシリコーン印象材を用いて、女性健常人の顔面の皮膚表面形態のレプリカを取り、実態顕微鏡にて観察した。表１４に示す基準に従って肌荒れ評価４と５と判断された者６０名を３班に分け、顔面左右半々に、実施例および比較例を１日２回ずつ１ヶ月間塗布した。
１ヶ月後、再び上記のレプリカ法によって顔面の皮膚表面形態を観察し、表１４の判定基準に従って評価した。第１班の結果を表１５に、第２班の結果を表１６に、第３班の結果を表１７に示す。
Test 3 Rough skin improvement test Hydrophilic Exaflex hydrophilic vinyl silicone impression material was used to take a replica of the skin surface morphology of a healthy female face and observed with a microscope. According to the criteria shown in Table 14, 60 persons judged to have rough skin evaluations 4 and 5 were divided into 3 groups, and Examples and Comparative Examples were applied to the left and right halves of the face twice a day for 1 month.
One month later, the skin surface morphology of the face was again observed by the above replica method, and evaluated according to the criteria shown in Table 14. The results of the first team are shown in Table 15, the results of the second team are shown in Table 16, and the results of the third team are shown in Table 17.
本発明の化粧料用原料は、８−ＯＨｄＧ形成抑制能およびコラーゲン合成促進能を有するため、肌荒れ改善効果の非常に高い化粧料を得ることができ、クリーム、乳液、ローション、パック、スプレー、ジェル、入浴剤、ファンデーション等の化粧品、医薬品、または医薬部外品として好適に利用できる。 Since the raw material for cosmetics of the present invention has an ability to suppress 8-OHdG formation and promotes collagen synthesis, it can obtain a cosmetic with a very high effect of improving rough skin, such as cream, emulsion, lotion, pack, spray, gel. , Bathing agents, foundations and other cosmetics, pharmaceuticals, or quasi-drugs.
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| JP2019011259A (en) * | 2017-06-29 | 2019-01-24 | 肇 小座野 | Filaggrin production promoter, loricrin production promoter, involucrin production promoter |
| JP2020125319A (en) * | 2015-09-03 | 2020-08-20 | 地方独立行政法人青森県産業技術センター | Anti-glycation agent and external skin preparation |
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