JP2009005625A - Mixing vessel and mixing kit - Google Patents

Mixing vessel and mixing kit Download PDF

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JP2009005625A
JP2009005625A JP2007170271A JP2007170271A JP2009005625A JP 2009005625 A JP2009005625 A JP 2009005625A JP 2007170271 A JP2007170271 A JP 2007170271A JP 2007170271 A JP2007170271 A JP 2007170271A JP 2009005625 A JP2009005625 A JP 2009005625A
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chamber
container
syringe
mixing
partition member
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JP5132207B2 (en
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Shuichi Akashi
秀一 明石
Masahiko Amano
雅彦 天野
Naoya Hosono
直哉 細野
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RIKEN Institute of Physical and Chemical Research
Toppan Inc
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Toppan Printing Co Ltd
RIKEN Institute of Physical and Chemical Research
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a mixing vessel which can easily and accurately mix a plurality of contents, while preventing contamination, and to provide a mixing kit. <P>SOLUTION: This mixing vessel 2 having the first chamber C1 and the second chamber C2 having an opening portion 11 comprises a partition member 10 disposed between the first chamber C1 and the second chamber C2 to separate the first chamber C1 and the second chamber C2 from each other, and a syringe-fixing portion disposed in the opening portion 11 and enabling the mounting of a syringe 3, wherein the partition member 10 can be pressed from the second chamber C2 side to make the first chamber C1 and the second chamber C2 communicate with each other. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

本発明は、異なる２種類以上の内容物を使用時に混合する混合容器及び混合キットに関する。   The present invention relates to a mixing container and a mixing kit for mixing two or more different contents at the time of use.

近年ヒトゲノム解析も終了し、遺伝子の機能解明が急速に行われている。それに伴い、これらの研究成果を利用した、遺伝子を用いる臨床診断も増加しつつある。これらの遺伝子検査を実施する際には、生体サンプルからの核酸抽出が必須である。   In recent years, human genome analysis has been completed, and the function of genes has been elucidated rapidly. Along with this, clinical diagnosis using genes using these research results is also increasing. When carrying out these genetic tests, it is essential to extract nucleic acids from biological samples.

一般的な核酸抽出法としては、細胞をホモジナイザー等の細胞破砕装置又は酵素等で処理後、カラムへ核酸を吸着させてエタノールで洗浄し、その後カラムから溶出させる（例えば、特許文献１参照。）。通常、このような作業において、多種類の試薬の混合及び遠心分離等が必要となることが多い。
特表平９−５０８６３８号公報 As a general nucleic acid extraction method, after treating cells with a cell disruption device such as a homogenizer or an enzyme, the nucleic acid is adsorbed onto the column, washed with ethanol, and then eluted from the column (see, for example, Patent Document 1). . Usually, in such operations, mixing of various types of reagents, centrifugation, and the like are often required.
JP 9-508638 A

しかしながら、特許文献１の方法は作業工程が多く煩雑であり、作業者への負担が大きいという問題がある。
また、作業がすべて開放系で行われるため、サンプル間のコンタミネーションや、試薬の取り違え等のヒューマンエラーによってサンプルが使用に耐えない状態となることがある等の問題もある。従って、特許文献１の方法は、作業者に一定の熟練が必要とされるのが現状である。 However, the method of Patent Document 1 has a problem that it has many work steps and is complicated, and the burden on the worker is large.
In addition, since all the work is performed in an open system, there is a problem that the sample may become unusable due to human error such as contamination between samples and mixing of reagents. Therefore, the method of patent document 1 is that the operator needs a certain skill now.

本発明は、上記事情に鑑みてなされたものであり、複数種の内容物を、汚染を防ぎながら容易かつ正確に混合し、一定量ずつ吐出することのできる混合容器及び混合キットを提供することを目的とする。   The present invention has been made in view of the above circumstances, and provides a mixing container and a mixing kit capable of mixing a plurality of types of contents easily and accurately while preventing contamination and discharging a predetermined amount. With the goal.

本発明の混合容器は、第１室と、開口部を有する第２室とを有する混合容器であって、
前記第１室と前記第２室との間に設けられ、前記第１室と前記第２室とを隔絶する隔壁部材と、前記開口部に設けられ、シリンジの装着を可能にするシリンジ固定部とを備え、前記隔壁部材が前記第２室側から押圧されることによって、前記第１室と前記第２室とを連通させることができることを特徴とする。 The mixing container of the present invention is a mixing container having a first chamber and a second chamber having an opening,
A partition wall member provided between the first chamber and the second chamber, which separates the first chamber from the second chamber, and a syringe fixing portion which is provided in the opening and enables a syringe to be attached. When the partition member is pressed from the second chamber side, the first chamber and the second chamber can be communicated with each other.

本発明の混合容器は、隔壁部材を押圧して開通させることによって、第１室と第２室とが連通し、第１室及び第２室の内容物を混合することが可能になる。   In the mixing container of the present invention, the first chamber and the second chamber communicate with each other by pressing the partition member and opening it, and the contents of the first chamber and the second chamber can be mixed.

前記第１室は、底面を有する第１容器によって形成されており、前記第２室は、第１端部に前記開口部が設けられ、第２端部に前記隔壁部材が装着された第２容器によって形成されており、前記第２容器の前記第２端部が前記第１容器に着脱自在に固定されて形成されてもよい。この場合、第２容器を異なる第２内容物が充填されたものと交換することによって、複数の組み合わせの混合を容易かつ確実に行うことができる。   The first chamber is formed by a first container having a bottom surface, and the second chamber has a second end provided with the opening at a first end and the partition member mounted at a second end. It may be formed by a container, and the second end of the second container may be detachably fixed to the first container. In this case, a plurality of combinations can be easily and reliably mixed by replacing the second container with one filled with a different second content.

前記隔壁部材は、前記開口部に向かって突出する突出部を有してもよい。この場合、隔壁部材を押圧しやすくなり、開通が容易になる。   The partition member may have a protrusion that protrudes toward the opening. In this case, it becomes easy to press the partition member, and opening is facilitated.

本発明の混合キットは、本発明の混合容器と、先端が前記シリンジ固定部に固定可能な形状を有するシリンジ外筒と、先端に突出して設けられた押圧部を有し、前記シリンジ外筒内に摺動可能に配置されたプランジャーと、を有するシリンジとを備え、前記シリンジは、吸引した内容物を所定量ずつ吐出可能に構成されており、前記シリンジを前記シリンジ固定部に装着したときに、前記押圧部が前記シリンジ外筒の先端から前記隔壁部材を押圧できる長さに突出可能であることを特徴とする。   The mixing kit of the present invention includes the mixing container of the present invention, a syringe outer cylinder having a shape whose tip can be fixed to the syringe fixing part, and a pressing part provided protruding from the tip, A syringe having a plunger slidably disposed on the syringe, and the syringe is configured to be able to discharge the sucked contents by a predetermined amount, and when the syringe is attached to the syringe fixing portion In addition, the pressing portion can protrude to a length capable of pressing the partition member from the tip of the syringe outer cylinder.

本発明の混合キットによれば、シリンジ外筒をシリンジ固定部に固定し、プランジャーの押圧部をシリンジ外筒の先端から突出させて隔壁部材を押圧することによって、混合操作を閉鎖系で行うことができる。また、混合後の内容物に対してそのまま分注等の作業を行うことができる。   According to the mixing kit of the present invention, the mixing operation is performed in a closed system by fixing the syringe outer cylinder to the syringe fixing section and causing the pressing portion of the plunger to protrude from the tip of the syringe outer cylinder and pressing the partition wall member. be able to. In addition, operations such as dispensing can be performed on the mixed contents as they are.

本発明の混合容器によれば、複数の内容物を、汚染を防ぎながら容易かつ正確に混合することができる。
本発明の混合キットによれば、内容物の混合操作を、異物の混入を防止して容易かつ正確に行い、混合物を一定量ずつ吐出することができる。 According to the mixing container of the present invention, a plurality of contents can be easily and accurately mixed while preventing contamination.
According to the mixing kit of the present invention, the mixing operation of the contents can be easily and accurately performed while preventing foreign matters from being mixed, and the mixture can be discharged by a certain amount.

以下、本発明の一実施形態の核酸抽出キット（混合キット）１について、図１から図８を参照して説明する。図１に示すように、核酸抽出キット１は、混合容器２と、シリンジ３とを備えて構成されている。
図２は、混合容器２を分解して示す図である。混合容器２は、第１容器４と、第１容器４に装着される第２容器５と、第２容器５の開口部に装着される蓋６とを備えて構成されている。 Hereinafter, a nucleic acid extraction kit (mixing kit) 1 according to an embodiment of the present invention will be described with reference to FIGS. As shown in FIG. 1, the nucleic acid extraction kit 1 includes a mixing container 2 and a syringe 3.
FIG. 2 is an exploded view of the mixing container 2. The mixing container 2 includes a first container 4, a second container 5 attached to the first container 4, and a lid 6 attached to the opening of the second container 5.

第１容器４は、ガラス又は樹脂等からなる略円筒状の容器であり、底面７と、開口した収容部８とを有する。第１容器４の材質としては、耐薬品性に優れた素材が好ましい。また、後述する使用時の操作による内圧の上昇が考えられるため、柔軟性を有する素材が好ましい。   The first container 4 is a substantially cylindrical container made of glass, resin, or the like, and has a bottom surface 7 and an opening accommodating portion 8. The material of the first container 4 is preferably a material excellent in chemical resistance. Moreover, since the raise of the internal pressure by the operation at the time of use mentioned later is considered, the raw material which has a softness | flexibility is preferable.

第２容器５は、樹脂等からなる略円筒状の容器部材９と、容器部材９に装着される隔壁部材１０とから構成されている。容器部材９の材質としては、耐薬品性を有するものが好ましい。
容器部材９の第１端部９Ａは開口しており、混合容器２の開口部１１を形成している。下方の第２端部９Ｂには、隔壁部材１０が嵌合されて装着されている。これによって、第２容器５は、全体として底面を有する容器となっている。隔壁部材１０は、耐薬品性に優れ、かつ柔軟性を有する素材から形成されるのが好ましい。例えばニトリルゴム等のゴムや、ポリプロピレン、ポリエチレン等の樹脂が好ましい。 The second container 5 includes a substantially cylindrical container member 9 made of resin or the like and a partition member 10 attached to the container member 9. As a material of the container member 9, a material having chemical resistance is preferable.
The first end 9 </ b> A of the container member 9 is open and forms the opening 11 of the mixing container 2. A partition wall member 10 is fitted and attached to the lower second end portion 9B. Accordingly, the second container 5 is a container having a bottom surface as a whole. The partition member 10 is preferably formed from a material having excellent chemical resistance and flexibility. For example, rubbers such as nitrile rubber and resins such as polypropylene and polyethylene are preferable.

第２容器５の外径は、第１容器４の収容部８の内径とほぼ同一となっており、第２容器５が収容部８に挿入されて圧入や接着等の手段によって一体に固定されている。第２容器５の外周面には、蓋６を装着するためのネジ溝１２が設けられており、開口部１１の内周面には、シリンジ３が装着されるネジ溝（シリンジ固定部）１３が設けられている。ネジ溝１３に代えて、公知のルアーロック等の固定機構を用いてもよい。   The outer diameter of the second container 5 is substantially the same as the inner diameter of the accommodating portion 8 of the first container 4, and the second container 5 is inserted into the accommodating portion 8 and fixed integrally by means such as press fitting or adhesion. ing. A screw groove 12 for mounting the lid 6 is provided on the outer peripheral surface of the second container 5, and a screw groove (syringe fixing portion) 13 in which the syringe 3 is mounted on the inner peripheral surface of the opening 11. Is provided. Instead of the thread groove 13, a known fixing mechanism such as a luer lock may be used.

従って、混合容器２の内部空間は、開口部１１と底面７との間に設けられた隔壁部材１０によって、隔壁部材１０と底面７を含む第１容器４とによって形成される第１室Ｃ１と、隔壁部材１０と開口部１１を含む第２容器５とによって形成される第２室Ｃ２との２つの空間に分割されている。そして、隔壁部材１０によって、第１室Ｃ１と第２室Ｃ２との間が隔絶されている。
第１室Ｃ１には、中和液Ｌ１が充填されており、第２室Ｃ２には抽出液Ｌ２が充填されている。 Therefore, the internal space of the mixing container 2 is defined by the first chamber C1 formed by the partition member 10 and the first container 4 including the bottom surface 7 by the partition member 10 provided between the opening 11 and the bottom surface 7. The second chamber 5 is divided into two spaces formed by the partition member 10 and the second container 5 including the opening 11. The first chamber C1 and the second chamber C2 are isolated from each other by the partition member 10.
The first chamber C1 is filled with the neutralization liquid L1, and the second chamber C2 is filled with the extraction liquid L2.

シリンジ３は、シリンジ外筒１４と、シリンジ外筒１４の内部に挿入されたプランジャー１５とで形成されている。
シリンジ外筒１４は、円筒状の本体部１６と、本体部１６の端部から延出する挿入部１７とを有している。挿入部１７の外径は、第２容器５の開口部１１の内径と略同一であり、外周面にネジ溝１３と係合するネジ山１７Ａが設けられている。従って、シリンジ外筒１４は開口部１１に装着固定可能に構成されている。また、シリンジ外筒１４の外面には、吸引した内容物を所定量ずつ吐出できるよう、図示しない目盛が設けられている。 The syringe 3 is formed by a syringe outer cylinder 14 and a plunger 15 inserted into the syringe outer cylinder 14.
The syringe outer cylinder 14 includes a cylindrical main body portion 16 and an insertion portion 17 that extends from an end portion of the main body portion 16. The outer diameter of the insertion portion 17 is substantially the same as the inner diameter of the opening 11 of the second container 5, and a screw thread 17 </ b> A that engages with the screw groove 13 is provided on the outer peripheral surface. Therefore, the syringe outer cylinder 14 is configured to be mounted and fixed to the opening 11. Moreover, the scale which is not shown in figure is provided in the outer surface of the syringe outer cylinder 14 so that the suctioned content can be discharged by predetermined amount.

プランジャー１５は、シリンジ外筒１４の本体部１６の内部を摺動可能に配置されており、先端に棒状の押圧部１９が突出して設けられている。押圧部１９の外径は、後述する吸引操作を可能にするために、押圧部１９が挿入部１７に挿通された状態で、通気できるだけの隙間を設けることが好ましい。このとき、押圧部１９の断面形状を、円でなく十字型等に形成することによって上記隙間を確保してもよい。   The plunger 15 is slidably disposed inside the main body portion 16 of the syringe outer cylinder 14 and is provided with a rod-shaped pressing portion 19 protruding from the tip. The outer diameter of the pressing portion 19 is preferably provided with a gap that allows ventilation in a state where the pressing portion 19 is inserted through the insertion portion 17 in order to enable a suction operation described later. At this time, the clearance may be secured by forming the cross-sectional shape of the pressing portion 19 in a cross shape instead of a circle.

プランジャー１５の基端はシリンジ外筒１４から常に突出しており、ユーザによって前進及び後退可能となっている。また、押圧部１９の基端付近には、シリンジ外筒１４の内径とほぼ同一の径を有するパッキン２０が設けられており、プランジャー１５を、シリンジ外筒１４内の任意の位置で止めることができるようになっている。   The base end of the plunger 15 always protrudes from the syringe outer cylinder 14 and can be moved forward and backward by the user. Further, a packing 20 having a diameter substantially the same as the inner diameter of the syringe outer cylinder 14 is provided near the proximal end of the pressing portion 19, and the plunger 15 is stopped at an arbitrary position in the syringe outer cylinder 14. Can be done.

上記のように構成された核酸抽出キット１の使用時の動作について、以下に説明する。
まず、混合容器２の蓋６を取り、図３に示すように、開口部１１からピペット１００等を用いて生体サンプルＳを抽出液Ｌ２に添加する。
生体サンプルＳとしては、対象の生物（生物の死体も含む）の血液、血漿、血清、***等の各種体液、皮膚、毛髪、筋肉組織等の各種組織、糞便、尿等の各種***物又はこれらに由来する試料等が用いられる。 The operation when using the nucleic acid extraction kit 1 configured as described above will be described below.
First, the lid 6 of the mixing container 2 is removed, and as shown in FIG. 3, the biological sample S is added to the extract L2 from the opening 11 using the pipette 100 or the like.
Examples of the biological sample S include various body fluids such as blood, plasma, serum, semen and the like, various tissues such as skin, hair, and muscle tissue, various excrements such as stool and urine, and the like. A sample derived from is used.

次に、図４に示すように、シリンジ外筒１４の挿入部１７のネジ山１７Ａを開口部１１の内側のネジ溝１３に係合させてシリンジ３を混合容器２に装着する。シリンジ３を装着した状態で混合容器２を振盪又は転倒攪拌、或いは攪拌機等を用いて機械的に攪拌し、生体サンプルＳ（不図示）と抽出液Ｌ２とを混合する。   Next, as shown in FIG. 4, the syringe 3 is mounted on the mixing container 2 by engaging the thread 17 </ b> A of the insertion portion 17 of the syringe outer cylinder 14 with the screw groove 13 inside the opening 11. With the syringe 3 attached, the mixing container 2 is shaken or overturned, or mechanically stirred using a stirrer or the like to mix the biological sample S (not shown) and the extract L2.

生体サンプルＳと抽出液Ｌ２とを充分に混合した後、ユーザはシリンジ３のプランジャー１５を押し込み、押圧部１９を挿入部１７から突出させて、隔壁部材１０を押圧する。   After the biological sample S and the extract L2 are sufficiently mixed, the user presses the plunger 15 of the syringe 3, causes the pressing portion 19 to protrude from the insertion portion 17, and presses the partition member 10.

隔壁部材１０は、押圧部１９に押圧されることによって、図５に示すように第２容器５の容器部材９との嵌合が外れて第１容器４内に落下する。こうして、第１室Ｃ１と第２室Ｃ２とが連通される。このとき、第２容器５内の抽出液Ｌ２及び生体サンプルＳも第１容器４内に移動して、中和液Ｌ１と混ざり合う。   When the partition member 10 is pressed by the pressing portion 19, the fitting of the second container 5 with the container member 9 is released and falls into the first container 4 as shown in FIG. 5. Thus, the first chamber C1 and the second chamber C2 are communicated. At this time, the extract L2 and the biological sample S in the second container 5 also move into the first container 4 and mix with the neutralizing liquid L1.

ユーザは、シリンジ３を装着したままシリンジ３及び混合容器２を保持して、転倒攪拌或いは攪拌機等を用いて機械的に攪拌し、中和液Ｌ１と抽出液Ｌ２、及び生体サンプルＳとを充分に攪拌する。   The user holds the syringe 3 and the mixing container 2 with the syringe 3 attached, and mechanically stirs by using an overturn stirrer or a stirrer, etc., and sufficiently supplies the neutralizing solution L1, the extract L2, and the biological sample S. To stir.

攪拌終了後、図６に示すように混合容器２を上にして中和液Ｌ１、抽出液Ｌ２、及び生体サンプルＳの混合物Ｌ３を開口部１１側に移動させ、プランジャー１５を引いて混合物Ｌ３をシリンジ外筒１４内に吸引回収する。衛生的に廃棄するために、使用後の混合容器２に蓋６を装着して、核酸抽出作業が終了する。
シリンジ３に吸引回収した混合物Ｌ３は、シリンジ外筒１４外面の目盛を参照しながらプランジャー１５を操作することによって、例えば遺伝子判定装置等に設置する反応容器のウェル等に、所定量ずつ吐出することができる。 After completion of the stirring, as shown in FIG. 6, the mixture L3 of the neutralizing solution L1, the extract L2, and the biological sample S is moved to the opening 11 side with the mixing container 2 facing up, and the plunger 15 is pulled to mix the mixture L3. Is sucked and collected in the syringe outer cylinder 14. In order to dispose of it hygienically, the lid 6 is attached to the used mixing container 2, and the nucleic acid extraction operation is completed.
The mixture L3 sucked and collected by the syringe 3 is discharged by a predetermined amount, for example, into a well of a reaction vessel or the like installed in a gene determination device or the like by operating the plunger 15 while referring to the scale on the outer surface of the syringe outer cylinder 14. be able to.

核酸抽出キット１を用いて抽出したゲノムと、核酸吸着カラムを使用する従来法を用いて抽出したゲノムとをサンプルに用いてポリメラーゼ連鎖反応法（ＰＣＲ）を行った結果図７に示す。図７において、Ｍは２０ｂｐラダーマーカー、Ｓ１は従来法によって精製したサンプルによるＰＣＲの結果、Ｓ２は核酸抽出キット１を用いて抽出したサンプルによるＰＣＲの結果をそれぞれ示す。
矢印で示すように、各サンプルにおいてほぼ同等のバンドが得られ、核酸抽出キット１による抽出サンプルが実際の検査において問題なく使用できることが示された。 FIG. 7 shows the result of polymerase chain reaction (PCR) using a sample extracted using the nucleic acid extraction kit 1 and a genome extracted using a conventional method using a nucleic acid adsorption column as a sample. In FIG. 7, M is a 20 bp ladder marker, S1 is the result of PCR using a sample purified by a conventional method, and S2 is the result of PCR using a sample extracted using the nucleic acid extraction kit 1.
As shown by the arrows, almost the same band was obtained in each sample, and it was shown that the sample extracted with the nucleic acid extraction kit 1 can be used without any problem in the actual examination.

本実施形態の核酸抽出キット１によれば、混合容器２の第２容器５の底面が、隔壁部材１０を嵌合させることによって形成されるので、第２室Ｃ２内で第１段階の混合調整を行った後に、プランジャー１５で隔壁部材１０を押圧することによって、第２室Ｃ２の抽出液Ｌ２と第１室Ｃ１の中和液Ｌ１とを容易に、かつ他の試薬等と取り違えることなく、確実に混合することができる。   According to the nucleic acid extraction kit 1 of the present embodiment, since the bottom surface of the second container 5 of the mixing container 2 is formed by fitting the partition member 10, the first stage of mixing adjustment in the second chamber C2 After performing the above, by pressing the partition member 10 with the plunger 15, the extraction liquid L2 in the second chamber C2 and the neutralization liquid L1 in the first chamber C1 can be easily mixed with other reagents and the like. Can be mixed reliably.

また、調整に使用する中和液Ｌ１と抽出液Ｌ２とが、必要量だけそれぞれ第１室Ｃ１と第２室Ｃ２とに充填されている。従って、定量分注器等の器具が必要なく、試薬の分量を間違えて投入してしまう等のヒューマンエラーの発生も防止することができる。   Further, the first chamber C1 and the second chamber C2 are filled with the necessary amounts of the neutralizing liquid L1 and the extraction liquid L2 used for the adjustment, respectively. Accordingly, there is no need for an instrument such as a quantitative dispenser, and it is possible to prevent the occurrence of a human error such as an incorrect amount of reagent being charged.

また、最終的に混合物Ｌ３を収容するシリンジ３が、混合容器２の開口部１１に装着されるので、混合容器２内の内容物にコンタミネーション等の汚染が発生しにくい。従って、衛生的に混合作業から混合物回収までの一連の作業を行うことができる。さらに、振盪時に混合容器２内の液体が飛散する等の事故も防止することができる。   Further, since the syringe 3 that finally contains the mixture L3 is attached to the opening 11 of the mixing container 2, contamination such as contamination is unlikely to occur in the contents in the mixing container 2. Therefore, a series of operations from the mixing operation to the mixture recovery can be performed hygienically. Furthermore, it is possible to prevent accidents such as liquid scattering in the mixing container 2 during shaking.

さらに、隔壁部材１０を開通させる押圧部１９がシリンジ３のプランジャー１５と一体に設けられているので、内容物の混合から混合物の吸引回収までの作業を閉鎖系において行うことができる。従って、汚染やヒューマンエラーの発生をさらに抑えることができる。   Furthermore, since the pressing portion 19 for opening the partition member 10 is provided integrally with the plunger 15 of the syringe 3, operations from mixing the contents to sucking and collecting the mixture can be performed in a closed system. Therefore, the occurrence of contamination and human error can be further suppressed.

本実施形態においては、隔壁部材１０がほぼ平坦な形状を有する例を説明したが、これに代えて、図８に示す変形例のように、隔壁部材２１の中央が開口部１１に向かって柱状に突出する突出部２１Ａを有してもよい。このようにすると、プランジャー１５の押圧部１９の長さを短くすることができ、核酸抽出キット１をより操作しやすく構成することができる。   In the present embodiment, an example in which the partition member 10 has a substantially flat shape has been described. Instead, the center of the partition member 21 has a columnar shape toward the opening 11 as in the modification shown in FIG. You may have the protrusion part 21A which protrudes. If it does in this way, the length of the press part 19 of the plunger 15 can be shortened, and the nucleic acid extraction kit 1 can be comprised more easily.

本実施形態においては、混合容器とシリンジとを有する混合キットとして構成された例を説明したが、本発明はこれには限定されず、混合容器のみの構成としてもよい。   In this embodiment, although the example comprised as a mixing kit which has a mixing container and a syringe was demonstrated, this invention is not limited to this, It is good also as a structure only of a mixing container.

以上、本発明の実施形態について説明してきたが、本発明の技術範囲は上記実施の形態に限定されるものではなく、本発明の趣旨を逸脱しない範囲において種々の変更を加えることが可能である。
例えば、混合容器を構成する第２容器は、着脱自在に第１容器に固定されてもよい。このようにすると、第１容器の内容物と混合されうる内容物が２種類以上ある際に、あらかじめ対象となる内容物が充填された第２容器を第１容器に固定することによって、混合容器又は混合キットの汎用性を向上させることができる。 Although the embodiments of the present invention have been described above, the technical scope of the present invention is not limited to the above-described embodiments, and various modifications can be made without departing from the spirit of the present invention. .
For example, the second container constituting the mixing container may be detachably fixed to the first container. In this case, when there are two or more types of contents that can be mixed with the contents of the first container, the second container filled with the target contents in advance is fixed to the first container, whereby the mixing container Or the versatility of a mixing kit can be improved.

また、上述の実施形態においては、混合容器が略円筒状の容器である例を説明したが、これには限定されず、例えば、スタンディングパウチのようにフィルム状の部材を張り合わせて形成されたものでもよい。このようにすると、廃棄の際の容積を小さくすることができる。   In the above-described embodiment, the example in which the mixing container is a substantially cylindrical container has been described. However, the present invention is not limited to this. For example, the mixing container is formed by pasting together film-like members such as a standing pouch. But you can. If it does in this way, the volume at the time of disposal can be made small.

さらに上述の実施形態においては、生体サンプルを添加して核酸抽出を行う例を説明したが、本発明の用途はこれには限定されず、使用直前の衛生的な混合操作を必要とする、例えばコンタクトレンズの洗浄液や、パーマ液、あるいはエポキシ樹脂等の混合操作にも使用することができる。さらに、混合の対象となる各内容物は液体に限定されず、例えばゲル状のものであってもよい。   Furthermore, in the above-described embodiment, an example of performing nucleic acid extraction by adding a biological sample has been described, but the application of the present invention is not limited to this, and requires a sanitary mixing operation immediately before use, for example, It can also be used for mixing contact lens cleaning liquid, permanent liquid, epoxy resin, or the like. Furthermore, each content to be mixed is not limited to a liquid, and may be, for example, a gel.

加えて、上述の実施形態においては、開口部１１が蓋６によって密閉されている例を説明したが、これに代えて、シート状の部材によって開口部が封止され、使用時に当該部材を破る或いは除去することによって開口部が開口されてもよい。   In addition, in the above-described embodiment, the example in which the opening 11 is sealed with the lid 6 has been described. Instead, the opening is sealed with a sheet-like member, and the member is broken during use. Or an opening part may be opened by removing.

本発明の一実施形態の核酸抽出キットを示す図である。It is a figure which shows the nucleic acid extraction kit of one Embodiment of this invention. 同核酸抽出キットの混合容器を分解して示す断面図である。It is sectional drawing which decomposes | disassembles and shows the mixing container of the nucleic acid extraction kit. 同核酸抽出キットの使用時の動作を示す図である。It is a figure which shows the operation | movement at the time of use of the nucleic acid extraction kit. 同核酸抽出キットの使用時の動作を示す図である。It is a figure which shows the operation | movement at the time of use of the nucleic acid extraction kit. 同核酸抽出キットの使用時の動作を示す図である。It is a figure which shows the operation | movement at the time of use of the nucleic acid extraction kit. 同核酸抽出キットの使用時の動作を示す図である。It is a figure which shows the operation | movement at the time of use of the nucleic acid extraction kit. 同核酸抽出キットによって得られたサンプルと、従来法によって得られたサンプルとのＰＣＲの結果を示す図である。It is a figure which shows the result of PCR of the sample obtained by the nucleic acid extraction kit, and the sample obtained by the conventional method. 同核酸抽出キットの変形例の混合容器を示す断面図である。It is sectional drawing which shows the mixing container of the modification of the nucleic acid extraction kit.

符号の説明Explanation of symbols

１ 核酸抽出キット（混合キット）
２ 混合容器
３ シリンジ
４ 第１容器
５ 第２容器
６ 蓋
７ 底面
８ 収容部
９ 容器部材
９Ａ 第１端部
９Ｂ 第２端部
１０、２１ 隔壁部材
１１ 開口部
１２ ネジ溝
１３ ネジ溝（シリンジ固定部）
１４ シリンジ外筒
１５ プランジャー
１６ 本体部
１７ 挿入部
１７Ａ ネジ山
１９ 押圧部
２０ パッキン
２１Ａ 突出部
Ｃ１ 第１室
Ｃ２ 第２室 1 Nucleic acid extraction kit (mixing kit)
2 Mixing container 3 Syringe 4 First container 5 Second container 6 Lid 7 Bottom face 8 Housing part 9 Container member 9A First end part 9B Second end part 10, 21 Bulkhead member 11 Opening part 12 Screw groove 13 Screw groove (Syringe fixing) Part)
14 Syringe outer cylinder 15 Plunger 16 Main body part 17 Insertion part 17A Thread 19 Press part 20 Packing 21A Protrusion part C1 First chamber C2 Second chamber

Claims (4)

第１室と、開口部を有する第２室とを有する混合容器であって、
前記第１室と前記第２室との間に設けられ、前記第１室と前記第２室とを隔絶する隔壁部材と、
前記開口部に設けられ、シリンジの装着を可能にするシリンジ固定部と、
を備え、
前記隔壁部材が前記第２室側から押圧されることによって、前記第１室と前記第２室とを連通させることができることを特徴とする混合容器。 A mixing container having a first chamber and a second chamber having an opening,
A partition member provided between the first chamber and the second chamber, and separating the first chamber and the second chamber;
A syringe fixing portion that is provided in the opening and enables mounting of a syringe;
With
The mixing container, wherein the first chamber and the second chamber can be communicated with each other by pressing the partition member from the second chamber side. 前記第１室は、底面を有する第１容器によって形成されており、
前記第２室は、第１端部に前記開口部が設けられ、第２端部に前記隔壁部材が装着された第２容器によって形成されており、
前記第２容器の前記第２端部が前記第１容器に着脱自在に固定されて形成されていることを特徴とする請求項１に記載の混合容器。 The first chamber is formed by a first container having a bottom surface;
The second chamber is formed by a second container in which the opening is provided at a first end and the partition member is mounted at a second end.
The mixing container according to claim 1, wherein the second end of the second container is formed to be detachably fixed to the first container. 前記隔壁部材は、前記開口部に向かって突出する突出部を有することを特徴とする請求項１又は２に記載の混合容器。   The mixing container according to claim 1, wherein the partition member has a protruding portion that protrudes toward the opening. 請求項１から３のいずれか１項に記載の混合容器と、
先端が前記シリンジ固定部に固定可能な形状を有するシリンジ外筒と、先端に突出して設けられた押圧部を有し、前記シリンジ外筒内に摺動可能に配置されたプランジャーと、を有するシリンジと、
を備え、
前記シリンジは、吸引した内容物を所定量ずつ吐出可能に構成されており、前記シリンジを前記シリンジ固定部に装着したときに、前記押圧部が前記シリンジ外筒の先端から前記隔壁部材を押圧できる長さに突出可能であることを特徴とする混合キット。 A mixing container according to any one of claims 1 to 3,
A syringe outer cylinder having a shape whose tip can be fixed to the syringe fixing part; and a plunger having a pressing part provided to protrude from the tip and slidably disposed in the syringe outer cylinder. A syringe,
With
The syringe is configured to be able to discharge the sucked contents by a predetermined amount, and when the syringe is attached to the syringe fixing portion, the pressing portion can press the partition member from the tip of the syringe outer cylinder. Mixing kit characterized in that it can protrude in length.
JP2007170271A 2007-06-28 2007-06-28 Mixing kit Active JP5132207B2 (en)

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Publication number Priority date Publication date Assignee Title
DE102011078855A1 (en) * 2011-07-08 2013-01-10 Siemens Aktiengesellschaft Device for the defined and metered supply of liquids
WO2015065011A1 (en) * 2013-10-29 2015-05-07 (주)차바이오메드 Centrifugation syringe
CN114317255A (en) * 2022-01-13 2022-04-12 珠海黑马生物科技有限公司 Totally-enclosed full-automatic nucleic acid extraction kit

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JPS62247829A (en) * 1986-02-26 1987-10-28 インテリジエント　メデイスン，インコ−ポレイテイド Mixer
US6098795A (en) * 1997-10-14 2000-08-08 Mollstam; Bo Device for adding a component to a package
JP2004041568A (en) * 2002-07-15 2004-02-12 Nipro Corp Two-ingredient mixture type prefilled syringe
JP2004236962A (en) * 2003-02-07 2004-08-26 Ajinomoto Faruma Kk Multi-chamber type medicine container
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011078855A1 (en) * 2011-07-08 2013-01-10 Siemens Aktiengesellschaft Device for the defined and metered supply of liquids
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WO2015065011A1 (en) * 2013-10-29 2015-05-07 (주)차바이오메드 Centrifugation syringe
CN114317255A (en) * 2022-01-13 2022-04-12 珠海黑马生物科技有限公司 Totally-enclosed full-automatic nucleic acid extraction kit
CN114317255B (en) * 2022-01-13 2022-11-04 珠海黑马生物科技有限公司 Totally-enclosed full-automatic nucleic acid extraction kit

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