JP2008061506A5 - - Google Patents

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JP2008061506A5
JP2008061506A5 JP2006239457A JP2006239457A JP2008061506A5 JP 2008061506 A5 JP2008061506 A5 JP 2008061506A5 JP 2006239457 A JP2006239457 A JP 2006239457A JP 2006239457 A JP2006239457 A JP 2006239457A JP 2008061506 A5 JP2008061506 A5 JP 2008061506A5
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acetoacetyl
synthase
coa
coa synthase
seq
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JP2006239457A
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JP4986547B2 (en
JP2008061506A (en
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アセチルＣｏＡとマロニルＣｏＡの両方を基質としてアセトアセチルＣｏＡを生成する反応を触媒する新規なアセトアセチルＣｏＡ合成酵素。   A novel acetoacetyl CoA synthase that catalyzes a reaction for producing acetoacetyl CoA using both acetyl CoA and malonyl CoA as substrates. アセトアセチルＣｏＡ合成酵素が、メバロン酸経路をもつ放線菌のメバロン酸経路遺伝子クラスター中に含まれるアセトアセチルＣｏＡ合成酵素遺伝子がコードするタンパク質である、請求項１記載のアセトアセチルＣｏＡ合成酵素。   The acetoacetyl CoA synthase according to claim 1, wherein the acetoacetyl CoA synthase is a protein encoded by an acetoacetyl CoA synthase gene contained in a mevalonate pathway gene cluster of actinomycetes having a mevalonate pathway. 配列番号１のアミノ酸配列。   The amino acid sequence of SEQ ID NO: 1. 配列番号１において、１から数個のアミノ酸が欠失、置換、付加及び／または挿入されているアミノ酸配列であって、アセトアセチルＣｏＡ合成酵素活性を有するアミノ酸配列。   An amino acid sequence in which 1 to several amino acids are deleted, substituted, added and / or inserted in SEQ ID NO: 1, and has acetoacetyl CoA synthetase activity. 配列番号１のアミノ酸配列と６０％以上の相同性を有するアミノ酸配列であって、アセトアセチルＣｏＡ合成酵素活性を有するアミノ酸配列。   An amino acid sequence having 60% or more homology with the amino acid sequence of SEQ ID NO: 1 and having acetoacetyl CoA synthetase activity. 配列番号２の塩基配列。   The base sequence of SEQ ID NO: 2. 配列番号２において、１〜数個の塩基配列が欠失、置換、付加及び/または挿入されている塩基配列であって、アセトアセチルＣｏＡ合成酵素を機能させるために必要な配列をすべてコードする塩基配列。   In SEQ ID NO: 2, a nucleotide sequence in which one to several nucleotide sequences are deleted, substituted, added and / or inserted, and which encodes all sequences necessary for functioning acetoacetyl CoA synthase An array. 配列番号２の塩基配列とストリンジェントな条件でハイブリダイズすることができる塩基配列であって、アセトアセチルＣｏＡ合成酵素を機能させるのに必要な配列をすべてコードする塩基配列。   A nucleotide sequence that can hybridize with the nucleotide sequence of SEQ ID NO: 2 under stringent conditions and encodes all sequences necessary for functioning acetoacetyl-CoA synthase. 請求項６〜８の何れかに記載の塩基配列を含むベクター。   A vector comprising the base sequence according to any one of claims 6 to 8. 請求項９に記載のベクターを有する形質転換体。   A transformant comprising the vector according to claim 9. 大腸菌である、請求項１０に記載の形質転換体。   The transformant according to claim 10, which is Escherichia coli. 請求項１０又は１１に記載の形質転換体を培養することを特徴とするアセトアセチルＣｏＡ合成酵素の製造方法。   A method for producing an acetoacetyl-CoA synthase, comprising culturing the transformant according to claim 10 or 11. 少なくとも、請求項１又は２に記載のアセトアセチルＣｏＡ合成酵素をコードする塩基配列又は請求項６〜８の何れかに記載の塩基配列を持つDNAを含むベクターで宿主を形質転換し、当該形質転換体内に少なくともアセトアセチルＣｏＡ合成酵素タンパク質を生成させ、少なくともアセトアセチルＣｏＡ合成酵素タンパク質の機能により、アセトアセチルＣｏＡから生合成される生理活性物質を蓄積させることを特徴とする生理活性物質の製造方法。  A host is transformed with a vector comprising at least the base sequence encoding the acetoacetyl-CoA synthetase according to claim 1 or 2 or the DNA having the base sequence according to any of claims 6 to 8, and the transformation A method for producing a physiologically active substance, characterized in that at least an acetoacetyl CoA synthase protein is produced in the body, and a physiologically active substance biosynthesized from acetoacetyl CoA is accumulated by at least the function of the acetoacetyl CoA synthase protein. 請求項１又は２に記載のアセトアセチルＣｏＡ合成酵素をコードする塩基配列、又は請求項６〜８の何れかに記載の塩基配列、３−ハイドロキシ−３−メチルグルタリルコエンザイムA合成酵素をコードする塩基配列及び３−ハイドロキシ−３−メチルグルタリルコエンザイムA還元酵素をコードする塩基配列の３種類の塩基配列を持つDNAを含むベクターで放線菌を形質転換し、該形質転換体の放線菌細胞中にアセトアセチルＣｏＡ合成酵素、３−ハイドロキシ−３−メチルグルタリルコエンザイムA合成酵素及び３−ハイドロキシ−３−メチルグルタリルコエンザイムＡ還元酵素の３種類の酵素タンパク質を生成させ、アセトアセチルＣｏＡ合成酵素タンパク質、３−ハイドロキシ−３−メチルグルタリルコエンザイムA合成酵素タンパク質および３−ハイドロキシ−３−メチルグルタリルコエンザイムA還元酵素タンパク質の機能によりメバロン酸を蓄積させることを特徴とする新規なメバロン酸の製造方法。   The base sequence encoding the acetoacetyl CoA synthase according to claim 1 or 2, or the base sequence according to any one of claims 6 to 8, encoding 3-hydroxy-3-methylglutaryl coenzyme A synthase Actinomycetes are transformed with a vector comprising a DNA having a base sequence and a DNA having three types of base sequences encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, Acetoacetyl-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, and 3-hydroxy-3-methylglutaryl coenzyme A reductase to produce three types of enzyme proteins. , 3-hydroxy-3-methylglutaryl coenzyme A synthase protein and And a novel method for producing mevalonic acid, characterized in that mevalonic acid is accumulated by the function of 3-hydroxy-3-methylglutaryl coenzyme A reductase protein.
JP2006239457A 2006-09-04 2006-09-04 Novel acetoacetyl CoA synthase, DNA sequence encoding the same, method for producing the enzyme, and method for producing mevalonic acid using the enzyme Active JP4986547B2 (en)

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JP2008061506A JP2008061506A (en) 2008-03-21
JP2008061506A5 true JP2008061506A5 (en) 2011-10-27
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JP2011518564A (en) 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク Isoprene synthase mutants for isoprene production by improved microorganisms
WO2010124146A2 (en) 2009-04-23 2010-10-28 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (en) 2009-05-08 2011-08-31 トヨタ自動車株式会社 Recombinant microorganism having butanol-producing ability and method for producing butanol
JP5630501B2 (en) * 2010-05-14 2014-11-26 トヨタ自動車株式会社 Isopropanol production method and recombinant yeast having isopropanol production ability
JP5056897B2 (en) * 2010-05-14 2012-10-24 トヨタ自動車株式会社 Method for producing 2-butanol and recombinant microorganism having 2-butanol-producing ability
US9217183B2 (en) 2010-05-14 2015-12-22 Toyota Jidosha Kabushiki Kaisha Method for producing isopropanol and recombinant yeast capable of producing isopropanol
US9273298B2 (en) 2010-10-27 2016-03-01 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
SG194690A1 (en) 2011-04-29 2013-12-30 Danisco Us Inc Recombinant microorganisms for enhanced production of mevalonate,isoprene, and isoprenoids
BR112014002661A2 (en) * 2011-08-04 2019-09-24 Danisco Us Inc isoprene production, isoprene precursors, and isoprenes with the use of acetoacetyl coa synthase
AU2012332996A1 (en) 2011-10-07 2014-04-24 Danisco Us Inc. Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
BR112014010053A8 (en) 2011-10-27 2017-06-20 Danisco Us Inc Isoprene synthase variants with enhanced solubility for isoprene production
EP2794876A1 (en) 2011-12-23 2014-10-29 Danisco US Inc. Production of isoprene under reduced oxygen inlet levels
BR112014014947A2 (en) 2011-12-23 2020-10-27 Danisco Us Inc. marked isoprene production with the use of host cells having decreased ispa activity
US9315831B2 (en) 2012-03-30 2016-04-19 Danisco Us Inc. Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
US8895277B2 (en) 2012-05-02 2014-11-25 Danisco Us Inc. Legume isoprene synthase for production of isoprene
WO2013181647A2 (en) 2012-06-01 2013-12-05 Danisco Us Inc. Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms
EP2935364A2 (en) 2012-12-21 2015-10-28 Danisco US Inc. Production of isoprene, isoprenoid, and isoprenoid precursors using an alternative lower mevalonate pathway
SG11201508186XA (en) 2013-04-10 2015-11-27 Danisco Us Inc Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids
WO2015127305A2 (en) 2014-02-20 2015-08-27 Danisco Us Inc. Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-coa-derived products

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