JP2007253131A - Functional water containing anti-oxidant - Google Patents
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Abstract
Description
本発明は、抗酸化剤を含有する機能水に関するものである。 The present invention relates to functional water containing an antioxidant.
特開２００３−１７５３９０号公報には、電解水素が溶存した機能水が開示されている。この機能水は、純水中に塩化ナトリウムを加え、電導率を１００μＳ／ｃｍ以上とした後に、電気分解し、陰極水を中性として得られるものである。
しかしながら、上記機能水の製造には、隔膜を備えた電解槽を使用しているため、陰極水はアルカリ性となり、ｐＨ調節が必要となる。このために、リン酸ナトリウムなどを用意しておき添加するのは手間が掛かる。また、ｐＨ調節のために陽極水を用いると、陽極水中の活性酸素が加えられてしまうので、好ましくない。
このため、本発明者らは、特開２００５−１８６０３４号公報に開示された水素水生成装置を開発した。この装置は、陽極と陰極との間を非隔膜状態で、電気分解を行い、発生した水素ガスを溜め置きし、その水素ガスを高濃度に水中に溶解することにより、機能水を調製するものである。
JP-A-2003-175390 discloses functional water in which electrolytic hydrogen is dissolved. This functional water is obtained by adding sodium chloride to pure water and setting the conductivity to 100 μS / cm or more and then electrolyzing it to make the cathode water neutral.
However, since an electrolytic cell equipped with a diaphragm is used for the production of the functional water, the cathode water becomes alkaline and pH adjustment is required. For this reason, it takes time and effort to prepare and add sodium phosphate and the like. Moreover, it is not preferable to use anodic water for pH adjustment because active oxygen in the anodic water is added.
For this reason, the present inventors have developed a hydrogen water generator disclosed in Japanese Patent Application Laid-Open No. 2005-186034. This device prepares functional water by performing electrolysis in a non-membrane state between the anode and the cathode, storing the generated hydrogen gas, and dissolving the hydrogen gas in water at a high concentration. It is.
しかし、本発明者らが開発に成功した機能水については、その特性および用途が十分に知られていなかった。
本発明は、上記した事情に鑑みてなされたものであり、その目的は、抗酸化剤を安定して含有する機能水を提供することにある。
However, the functional water that the inventors have successfully developed has not been sufficiently known for its properties and uses.
This invention is made | formed in view of an above-described situation, The objective is to provide the functional water which contains an antioxidant stably.
一般にビタミンＣに代表される抗酸化剤は高ｐＨ領域では非常に不安定であり、従来のアルカリイオン水に抗酸化剤を溶解すると、数時間〜数十時間の後には、抗酸化剤が分解してしまうため、その抗酸化剤の効果を十分に発揮させることが困難であった。ところが、本発明者らの検討によれば、特定の製造法により製造した水を用いることにより、そのように不安定な抗酸化剤を長時間に渡って安定して保持できることを見出し、基本的には本発明を完成するに至った。
上記課題を解決するために、第１の発明に係る機能水は、ｐＨが６から８までの中性水（以下、ｐＨが６〜８までの中性領域にある機能水を「中性水」と略記する）と、該中性水を電気分解し発生した水素ガスを溜め置きして、該中性水に溶存させることで高濃度に水素ガスを含ませた水であって、その物理化学的特性は、酸化還元電位が−２００ｍＶ以下であり、溶存水素濃度が０.５ｍｇ／Ｌ以上であり、かつ抗酸化剤を含有することを特徴とする。
In general, antioxidants typified by vitamin C are very unstable in the high pH range, and when antioxidants are dissolved in conventional alkaline ionized water, the antioxidants are decomposed after several hours to several tens of hours. For this reason, it has been difficult to sufficiently exert the effect of the antioxidant. However, according to the study by the present inventors, it has been found that by using water produced by a specific production method, such an unstable antioxidant can be stably maintained over a long period of time. The present invention has been completed.
In order to solve the above-mentioned problem, the functional water according to the first invention is neutral water having a pH of 6 to 8 (hereinafter, functional water in a neutral region of
上記発明において、前記抗酸化剤は、ビタミンＣ、ビタミンＥ、ビタミンＡ、カテキン類、コエンザイムＱ１０、フラボノイド、アントシアニン、プロアントシアニジン、シリマリン、ヘスペリジン、ルチン、アルファリポ酸、フラバンジェノール、ＳＯＤ、アルファカロチン、ベータカロチン、セレン、マンガン、亜鉛、システイン、グルタチオン、メラトニン、エンゾジノール、ピクノジェノール、リコペン、ルテインから選ばれる一つまたはそれ以上の物質を含むことが好ましい。 In the above invention, the antioxidant is vitamin C, vitamin E, vitamin A, catechins, coenzyme Q10, flavonoids, anthocyanins, proanthocyanidins, silymarin, hesperidin, rutin, alpha lipoic acid, flavangenol, SOD, alpha carotene , Beta carotene, selenium, manganese, zinc, cysteine, glutathione, melatonin, enzogenol, pycnogenol, lycopene, lutein.
本発明に関する機能水を製造する装置では、電解ガスを溜め置きする手法を用いているため、陽極側で発生する活性酸素は自己消失して酸素に戻るか、あるいは後段の活性炭フィルタで除去することで、機能水中に活性酸素が含有することを防止している。
本発明の機能水を使用するについては、例えば経口、注射、点滴、経直腸などの方法を用いて体内に摂取することができる。
In the apparatus for producing functional water according to the present invention, since a method of storing electrolytic gas is used, the active oxygen generated on the anode side is self-dissipated to return to oxygen, or removed by a subsequent activated carbon filter. Thus, active oxygen is prevented from being contained in the functional water.
As for the use of the functional water of the present invention, it can be taken into the body using methods such as oral, injection, infusion, and rectal.
このうち、経口摂取することが最も好ましい。この場合には、１日あたり、２０ミリリットル／キログラム（体重）〜７０ミリリットル／キログラム（体重）（好ましくは、４０ミリリットル／キログラム（体重）〜５０ミリリットル／キログラム（体重））の機能水を飲むことにより摂取を行う。なお、機能水をそのまま飲用するにも、例えば料理用の水として用いることができる。また、機能水は１日に数回に分けて摂取することができる。
機能水の経口摂取が困難な場合には、点滴溶液そのものとして、或いは各種医薬品などを調製するための水として機能水を利用することができる。この場合には、１日あたり、５ミリリットル／キログラム（体重）〜７０ミリリットル／キログラム（体重）（好ましくは、１５ミリリットル／キログラム（体重）〜３０ミリリットル／キログラム（体重））の機能水を点滴することができる。
Of these, oral intake is most preferred. In this case, drink functional water of 20 ml / kg (body weight) to 70 ml / kg (body weight) (preferably 40 ml / kg (body weight) to 50 ml / kg (body weight)) per day. Ingest by. In addition, when drinking functional water as it is, it can be used, for example, as cooking water. Functional water can be taken in several divided doses per day.
When oral intake of functional water is difficult, functional water can be used as an infusion solution itself or as water for preparing various pharmaceuticals. In this case, 5 ml / kg (body weight) to 70 ml / kg (body weight) (preferably 15 ml / kg (body weight) to 30 ml / kg (body weight)) of functional water is infused per day. be able to.
本発明によれば、高い溶存水素濃度と低酸化還元電位を示し、高い活性酸素除去能力を備えると共に、一般には分解しやすい抗酸化剤を効率良く保持する活性を備えた新規な機能水が提供される。 According to the present invention, there is provided a novel functional water that exhibits a high dissolved hydrogen concentration and a low redox potential, has a high active oxygen removal capability, and generally has an activity of efficiently retaining an antioxidant that is easily decomposed. Is done.
次に、本発明の実施形態について、図面を参照しつつ説明するが、本発明の技術的範囲は、これらの実施形態によって限定されるものではなく、発明の要旨を変更することなく様々な形態で実施することができる。また、本発明の技術的範囲は、均等の範囲にまで及ぶものである。 Next, embodiments of the present invention will be described with reference to the drawings. However, the technical scope of the present invention is not limited by these embodiments, and various forms are possible without changing the gist of the invention. Can be implemented. Further, the technical scope of the present invention extends to an equivalent range.
＜機能水の物理化学的特性＞
機能水の調製は、特開２００５−１８６０３４号公報に開示された技術を用いて作成した装置により行った。この装置及び機能水の特性を次のようにして確認した。
１．調製された機能水の溶存水素濃度および酸化還元電位の測定
電解条件が２００ｍＡｘ６時間、電解圧力が０.０４ＭＰａ、給水圧力が０.１８ＭＰａ、水温が２７℃の条件で機能水を調製した。この装置から機能水を水量が３.５Ｌ／ｍｉｎ、空間速度（ＳＶ）が１４０ｈｒ−１となる速度で通水したときの時間と、溶存水素・溶存酸素（ｍｇ／Ｌ）および酸化還元電位（ｍＶ）との関係を測定した。
結果を図１に示した。このときの機能水のｐＨは６.６とほぼ中性であった。
<Physicochemical properties of functional water>
The functional water was prepared by an apparatus prepared using the technique disclosed in Japanese Patent Application Laid-Open No. 2005-186034. The characteristics of this apparatus and functional water were confirmed as follows.
1. Measurement of dissolved hydrogen concentration and oxidation-reduction potential of prepared functional water Functional water was prepared under conditions of electrolysis of 200 mA × 6 hours, electrolysis pressure of 0.04 MPa, feed water pressure of 0.18 MPa, and water temperature of 27 ° C. Functional water was passed from this device at a rate of water volume of 3.5 L / min and space velocity (SV) of 140 hr −1 , dissolved hydrogen / dissolved oxygen (mg / L) and redox potential ( mV) was measured.
The results are shown in FIG. The pH of the functional water at this time was almost neutral at 6.6.
２．調製後に放置された装置中の機能水の溶存水素濃度の測定
上記と同様の条件によって、機能水を調製した後に、１４℃または１６℃において、０.５時間〜６０時間の間、装置中に放置された機能水を貯留した装置に通水を行ったときの通水中の溶存水素濃度を測定した。
結果を図２に示した。
2. Measurement of dissolved hydrogen concentration in functional water left in the apparatus after preparation After the functional water was prepared under the same conditions as described above, it was placed in the apparatus at 14 ° C or 16 ° C for 0.5 to 60 hours. The dissolved hydrogen concentration in the water was measured when water was passed through the device storing the left functional water.
The results are shown in FIG.
また、通水量が２.２Ｌのときの溶存水素量（図２において、ほぼピーク時の溶存水素量に該当する）と、放置時間との関係を図３に示した。
本実施形態に用いた機能水製造装置においては、電解水素を貯留する形式で製造する。このため、装置に通水し始めると、時間に応じて、通水中の溶存水素濃度は減少し、酸化還元電位および溶存酸素濃度は上昇する。
Further, FIG. 3 shows the relationship between the dissolved hydrogen amount when the water flow rate is 2.2 L (corresponding to the dissolved hydrogen amount at the peak in FIG. 2) and the standing time.
In the functional water manufacturing apparatus used for this embodiment, it manufactures in the form which stores electrolytic hydrogen. For this reason, when water begins to flow through the apparatus, the dissolved hydrogen concentration in the water decreases with time, and the oxidation-reduction potential and the dissolved oxygen concentration increase.
一般に、家庭で水道水を飲食用として用いる場合には、通水開始からせいぜい１分間程度（容量として２〜３Ｌ程度）で通水処理を完了する。このため、３Ｌ程度の量が流れたとき（図１において、約０.８ｍｉｎの位置）に、十分な溶存水素濃度と酸化還元電位とを維持していれば、機能水は、その効能を十分に備えた状態のままである。
図１〜図３によれば、機能水の溶存水素濃度は、そのような一般的な使用条件において、最高で０.８ｍｇ／Ｌ以上であった。また、このときの酸化還元電位は、−２００ｍＶよりも低かった。
In general, when tap water is used for eating and drinking at home, the water flow treatment is completed in about 1 minute (about 2 to 3 L as capacity) from the start of water flow. For this reason, if an amount of about 3 L flows (position of about 0.8 min in FIG. 1), if sufficient dissolved hydrogen concentration and oxidation-reduction potential are maintained, the functional water has sufficient effect. It remains in the state prepared for.
According to FIGS. 1 to 3, the dissolved hydrogen concentration of the functional water was at most 0.8 mg / L or more under such general use conditions. Moreover, the oxidation-reduction potential at this time was lower than -200 mV.
＜機能水の活性酸素除去能確認試験＞
次に、純水、アルカリイオン水、及び本実施形態の機能水について、活性酸素除去能を比較した。
アルカリイオン水として、医療認可を受けた製造元の異なる２種類のアルカリイオン整水器を選定し、その整水器によって製造されたものを用いた。これらのアルカリイオン水は、水中に乳酸カルシウムなどのイオン源を加えて電解する従来の方法によって製造されたものである。
また、機能水として、溶存水素濃度が０.８ｍｇ／Ｌ以上、酸化還元電位が−２００ｍＶ以下、ｐＨが約７のものを用いた。
<Functional water active oxygen removal ability confirmation test>
Next, the active oxygen removal ability was compared for pure water, alkaline ionized water, and functional water of this embodiment.
As alkaline ionized water, two types of alkaline ionized water adjusters with different manufacturers who received medical approval were selected, and those manufactured by the water conditioner were used. These alkaline ionized waters are produced by a conventional method in which an ion source such as calcium lactate is added to water for electrolysis.
As the functional water, one having a dissolved hydrogen concentration of 0.8 mg / L or more, an oxidation-reduction potential of −200 mV or less, and a pH of about 7 was used.
上記３種類の水中に活性酸素源として過酸化水素を添加し、残存するヒドロキシラジカルをＥＳＲで測定した。
結果を図４に示した。なお、２種類のアルカリイオン水では、ほぼ同等の結果が得られたため、図４（Ｂ）に代表的なグラフを示した。
図より、ヒドロキシラジカル濃度に該当するピーク値（Ｒ）を相対的に比較すると、純水１００％に対し、アルカリイオン水では４８％であったのに対し、機能水では３２％であった。このことから、従来の方法で得られたアルカリイオン水に比べ、本実施形態の機能水は、より強い抗酸化力を持っていることが示された。
Hydrogen peroxide was added as an active oxygen source to the above three types of water, and the remaining hydroxy radicals were measured by ESR.
The results are shown in FIG. In addition, since almost the same result was obtained with two types of alkaline ionized water, a representative graph is shown in FIG.
From the figure, when the peak value (R) corresponding to the hydroxy radical concentration is relatively compared, it was 48% for alkaline ionized water and 32% for functional water compared to 100% for pure water. From this, it was shown that the functional water of this embodiment has stronger antioxidant power than the alkali ion water obtained by the conventional method.
＜ビタミンＣ保持効果確認試験＞
次に、アルカリイオン水と機能水とについて、ビタミンＣを保持する効果を比較した。
アルカリイオン水として、医療認可を受けた製造元の異なる２種類のアルカリイオン整水器を選定し、その整水器によって調製されたものを用いた。
また、機能水として、初期水質が溶存水素濃度０.８ｍｇ／Ｌ、酸化還元電位−２００ｍＶのものを用い、この機能水中にビタミンＣ源としてアスコルビン酸を溶解させた。なお、試験開始時における溶液中の溶存水素濃度は０.５ｍｇ／Ｌ、酸化還元電位−２００ｍＶであった。
アスコルビン酸の初期濃度は、８０μＭとなるようにアルカリイオン水または機能水によって希釈した。この溶液をバイアルに対して適当量に分注した後、３７℃インキュベータに投入した。投入後、６時間及び３０時間して、バイアルをインキュベータから取り出し、ビタミンＣの酸化を防ぐために５０μＭジチオスレイトールを添加し、４℃にて保存した。
<Vitamin C retention effect confirmation test>
Next, the effect of retaining vitamin C was compared between alkaline ionized water and functional water.
As alkaline ionized water, two types of alkaline ionized water purifiers with different manufacturers who received medical approval were selected, and those prepared by the water purifier were used.
Further, as the functional water, an initial water quality having a dissolved hydrogen concentration of 0.8 mg / L and an oxidation-reduction potential of −200 mV was used, and ascorbic acid was dissolved as a vitamin C source in the functional water. The dissolved hydrogen concentration in the solution at the start of the test was 0.5 mg / L, and the oxidation-reduction potential was -200 mV.
The initial concentration of ascorbic acid was diluted with alkaline ionized water or functional water so as to be 80 μM. This solution was dispensed into a vial in an appropriate amount and then put into a 37 ° C. incubator. At 6 and 30 hours after addition, the vials were removed from the incubator and 50 μM dithiothreitol was added to prevent vitamin C oxidation and stored at 4 ° C.
全ての反応が終了後、各サンプル溶液中のビタミンＣ濃度をＨＰＬＣによって測定した。ＨＰＣＬ測定用の移動相として、０.１Ｍ ＫＨ２ＰＯ４−Ｈ３ＰＯ４＋０.１ｍＭ ＥＤＴＡ−２Ｎａを用いた。
結果を図５に示した。図より、アルカリイオン水では、６時間後に３８％、３０時間後には１４％の残存率となっており、ビタミンＣが急速に分解されたことを示した。一方、機能水では、６時間後に９６％、３０時間後には６７％と、非常に良好な残存率を示した。このことより、機能水は、アルカリイオン水に比べて、非常に高いビタミンＣ保持効果を示すことが分かった。
After all the reactions were completed, the vitamin C concentration in each sample solution was measured by HPLC. As the mobile phase for HPCL measurements, using 0.1M KH 2 PO 4 -H 3 PO 4 + 0.1mM EDTA-2Na.
The results are shown in FIG. From the figure, it was found that in alkaline ionized water, the residual rate was 38% after 6 hours and 14% after 30 hours, indicating that vitamin C was rapidly decomposed. On the other hand, functional water showed a very good residual rate of 96% after 6 hours and 67% after 30 hours. From this, it was found that functional water exhibits a very high vitamin C retention effect as compared with alkaline ionized water.
このように、本実施形態によれば、０.５ｍｇ／Ｌ以上の高い溶存水素濃度、−２００ｍＶ以下の酸化還元電位、ｐＨが６〜８を示す機能水に抗酸化剤を含有させることにより、長期間に渡って安定して抗酸化剤を保持できることが分かった。このような抗酸化剤含有機能水は、人体の酸化を防止する作用を有していることから、健康を維持するための水として有効に用いることができる。 Thus, according to this embodiment, by adding an antioxidant to functional water having a high dissolved hydrogen concentration of 0.5 mg / L or higher, a redox potential of −200 mV or lower, and a pH of 6 to 8, It was found that the antioxidant can be retained stably over a long period of time. Such an antioxidant-containing functional water can effectively be used as water for maintaining health because it has an action of preventing oxidation of the human body.
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Cited By (5)
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| KR100982977B1 (en) * | 2007-11-21 | 2010-09-17 | 윤충한 | Treating composition for tap water |
| JP2013532661A (en) * | 2010-07-22 | 2013-08-19 | リベン ファーマシューティカルズ インコーポレイテッド | Methods of treating or ameliorating diseases and methods of improving behavior involving the use of magnetic dipole stabilization solutions |
| JP2016172256A (en) * | 2016-07-01 | 2016-09-29 | 有限会社 シリーズ | Production device for active aqueous solution for animal or plant organism |
| CN110683627A (en) * | 2019-09-23 | 2020-01-14 | 佘建华 | Functional water containing SOD and preparation method thereof |
| US11110053B2 (en) | 2008-07-25 | 2021-09-07 | Reven Pharmaceuticals Inc. | Compositions and methods for the prevention and treatment of cardiovascular diseases |
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