JP2007167057A5 - Inspection method of weak light sample - Google Patents

Inspection method of weak light sample Download PDF

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JP2007167057A5
JP2007167057A5 JP2006124482A JP2006124482A JP2007167057A5 JP 2007167057 A5 JP2007167057 A5 JP 2007167057A5 JP 2006124482 A JP2006124482 A JP 2006124482A JP 2006124482 A JP2006124482 A JP 2006124482A JP 2007167057 A5 JP2007167057 A5 JP 2007167057A5
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weak light
image
light
sample
weak
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JP5265092B2 (en
JP2007167057A (en
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生体試料を含む検体を画像取得可能な状態に準備し、前記検体からの生物学的活性に起因する微弱な光学的データを蓄積して画像解析可能な画像情報を取得する微弱光画像取得手段と連携し、前記微弱光画像を形態的に解析して前記検体中における個々の細胞を認識するとともに、認識した個々の細胞に対応する微弱光の光強度を網羅的に評価するための画像解析を行うことを特徴とする微弱光検体の検査方法。 Weak light image acquisition means for preparing a sample including a biological sample in a state capable of image acquisition, accumulating weak optical data resulting from biological activity from the sample and acquiring image information capable of image analysis In cooperation with each other, the weak light image is morphologically analyzed to recognize individual cells in the sample, and image analysis for comprehensively evaluating the light intensity of the weak light corresponding to the recognized individual cells An inspection method of a weak light sample characterized by performing. 前記微弱光画像取得手段がさらに異なる所望の経過時間ごとの微弱光画像を連続的ないし断続的に取得する構成を有する場合であって、１以上の同一の細胞に関する時間ごとの前記生物学的活性に起因する光学的データの光強度を網羅的に解析することを特徴とする請求項１に記載の微弱光検体の検査方法。 In the case where the weak light image acquisition means has a configuration for continuously or intermittently acquiring a different weak light image for each desired elapsed time, the biological activity with respect to one or more same cells per time can be obtained. The method according to claim 1, wherein the light intensity of the optical data resulting from the light is comprehensively analyzed. 画像取得した時刻に応じた評価を行う工程をさらに有する請求項２に記載の微弱光検体の検査方法。The method of testing a weak light sample according to claim 2, further comprising the step of performing an evaluation according to the time when the image is acquired. 前記微弱光画像取得手段が取得した時間ごとの光学的データに基づく動画を生成することを特徴とする請求項３に記載の微弱光検体の検査方法。The inspection method of a weak light specimen according to claim 3, wherein a moving image is generated based on optical data for each time acquired by the weak light image acquisition means. 前記画像解析した２以上の細胞の光強度および／または光強度パターンに応じて同一または異なる細胞グループに分類することを特徴とする請求項１または２に記載の微弱光検体の検査方法。 The method for testing a weak light specimen according to claim 1 or 2 , wherein the light intensities and / or light intensity patterns of two or more cells subjected to the image analysis are classified into the same or different cell groups.
JP2006124482A 2005-11-22 2006-04-27 Method for testing faint light specimens Expired - Fee Related JP5265092B2 (en)

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JP2006124482A JP5265092B2 (en) 2005-11-22 2006-04-27 Method for testing faint light specimens

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JP2007167057A JP2007167057A (en) 2007-07-05
JP2007167057A5 true JP2007167057A5 (en) 2009-06-18
JP5265092B2 JP5265092B2 (en) 2013-08-14

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JP5307539B2 (en) 2006-05-31 2013-10-02 オリンパス株式会社 Biological sample imaging method and biological sample imaging apparatus
JP5124216B2 (en) * 2007-09-14 2013-01-23 オリンパス株式会社 Optical signal observation method and optical signal observation system
JP5307426B2 (en) * 2008-03-27 2013-10-02 オリンパス株式会社 Complement activity test method
US20100247446A1 (en) * 2009-03-24 2010-09-30 Olympus Corporation Method and system for analyzing optical signal
JPWO2016110980A1 (en) * 2015-01-08 2017-10-12 オリンパス株式会社 Image acquisition apparatus and image acquisition method
CN105092564B (en) * 2015-08-07 2018-06-12 南京理工大学 A kind of ZnTCPP/TOAB lighting electrodes, preparation method and its application in luminescence imaging platform

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WO2000007007A1 (en) * 1998-07-28 2000-02-10 Biometric Imaging, Inc. Device and method for cell motility assay
JP2002310894A (en) * 2001-04-11 2002-10-23 Japan Science & Technology Corp Emission observing device, emitter sorting device and sorting method for emitter
US20030211548A1 (en) * 2002-01-29 2003-11-13 Oncolmmunin, Inc. Visualization and quantitiation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers
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JP4454998B2 (en) * 2003-09-29 2010-04-21 株式会社ニコン Cell observation apparatus and cell observation method
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CN102156346B (en) * 2005-02-21 2012-07-18 奥林巴斯株式会社 Weak-light specimen imaging unit and weak-light specimen imaging device
JP5143348B2 (en) * 2005-10-18 2013-02-13 オリンパス株式会社 Apparatus for long-term evaluation of biological activity or method of automatic analysis
JP5089936B2 (en) * 2006-07-26 2012-12-05 オリンパス株式会社 Detection method of luminescence imaging

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