JP2006519285A - ポリマー合成のためのシステムおよび方法 - Google Patents
ポリマー合成のためのシステムおよび方法 Download PDFInfo
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- JP2006519285A JP2006519285A JP2006501979A JP2006501979A JP2006519285A JP 2006519285 A JP2006519285 A JP 2006519285A JP 2006501979 A JP2006501979 A JP 2006501979A JP 2006501979 A JP2006501979 A JP 2006501979A JP 2006519285 A JP2006519285 A JP 2006519285A
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Abstract
Description
a)複数の官能基を備えるポリマー表面を調製し、
b)前記表面の上に、第一のポリマー構築ブロックからなる第一の微小液滴をアプライし、
c)第一のポリマー構築ブロックをポリマー表面の官能基と反応させて、第一のポリマー構築ブロックをポリマー表面に付着させ、
d)該付着したポリマー構築ブロックの保護基を除去し、
e)前記第一の微小液滴の上に、第一のポリマー構築ブロックとは異なる、あるいはそれと同じ、第二のポリマー構築ブロックからなる第二の微小液滴をアプライし、
f)前記第二のポリマー構築ブロックを、第一のポリマー構築ブロックの脱保護した反応基と反応させ、
g)必要に応じて手順d)−f)を連続して繰り返す、
という手順からなる方法であり、
手順c)の後で、ポリマー表面の未反応の官能基は、化学的に不活性の化学種に変換される。
ポリマー構築ブロック:ポリマー構築ブロック(あるいは「シントン」)という用語は、最終的なポリマーの中に含まれる化学成分を指すものである。それゆえ、その化学成分には、最終的なポリマーの中に組み込まれる前の官能基が含まれることがある。本発明におけるポリマー構築ブロックとして相応しいものを、非限定的な例として列挙すると、置換されたホスホアミダイト、または非置換のホスホアミダイト、モノヌクレオチド、オリゴヌクレオチド、およびポリヌクレオチド、アミノ酸、ペプチド、糖(フラノース、リボース等)、ビオチン、アビジン、ストレプトアビジン、抗体などがある。
図1は、本発明のシステムの望ましい実施態様の一つを概略的に示している。
図2は、本発明のインクジェット・プリント・ヘッドを概略的に示している。
図3は、本発明のシステムの実施態様の一つをさらに概略的に示している。
図4は、本発明のシステムのさらに望ましい実施態様の一つを示している。
図5は、本発明のバイオチップの断面図を概略的に示している。
図6は、本発明のシステムの実施態様の一つをさらに概略的に示している。
図7は、本発明の方法の第一の反応手順を概略的に示している。
図8は、本発明の方法の第二の反応手順、およびその後に続く反応手順を概略的に示している。
1.ホスホアミダイト+活性剤の混合、基板上への噴射、洗浄、酸化、洗浄、表面の未反応官能基の化学的に不活性な化学種への変換、洗浄、脱保護、洗浄、フラッシング、および乾燥。
2.第二のホスホアミダイト+活性剤の混合、基板上への噴射、洗浄、酸化、洗浄、キャップ形成、洗浄、脱保護、洗浄、フラッシング、および乾燥、手順2を必要な頻度で反復。
3.NH3の添加、洗浄;終了。
102、317 基板
107〜110、308〜311、403 微小混合容器
111 輸送手段
112 処理室
200、304〜307 インクジェット・プリント・ヘッド
207 ノズル
401、402 タンク
Claims (24)
- ポリマー構築ブロックを連続して添加することにより、ポリマー鎖を固体基板の上に合成するための自動化されたポリマー合成システムであって、
a)ポリマー構築ブロックを含む微小液滴の発生を制御するためのノズルを複数備えたインクジェット・プリント・ヘッドと、
b)微小液滴を流体と共に載せた基板を処理するための処理装置と、
c)基板をプリント・ヘッドに隣接するようにおよび処理装置に隣接するように輸送させるための輸送手段と、
d)プリント・ヘッドに隣接した微小混合容器であって、この中でポリマー構築ブロックを活性剤と混合することで、一回の反応手順で反応させる量の構築ブロックを含むようにした、微小混合容器、
という要素からなる、システム。 - 要素を直線状に配置していることを特徴とする、請求項1に記載のシステム。
- 要素を円形に配置していることを特徴とする、請求項1に記載のシステム。
- 複数のノズルを直線上に配置していることを特徴とする、請求項1〜請求項3のいずれか一つに記載のシステム。
- 各ノズルが個別に供給可能であることを特徴とする、請求項4に記載のシステム。
- インクジェット・ヘッドに各ノズルと接続されたタンクが一つあることを特徴とする、請求項1〜請求項5のいずれか一つに記載のシステム。
- 各構築ブロックを別個のタンクに収納することを特徴とする、請求項6に記載のシステム。
- 各活性剤を別個のタンクに収納することを特徴とする、請求項6または請求項7に記載のシステム。
- バイオチップまたは遺伝子チップの製造のための、請求項1〜請求項8のいずれか一つに記載のシステムの使用法。
- 除去可能な保護基で保護された反応基を含むポリマー構築ブロックを連続して反応させることからなる、固体基板上にポリマーを合成する方法であり、
a)複数の官能基を備えるポリマー表面を調製し、
b)前記表面の上に、第一のポリマー構築ブロックからなる第一の微小液滴をアプライし、
c)第一のポリマー構築ブロックをポリマー表面の官能基と反応させて、第一のポリマー構築ブロックをポリマー表面に付着させ、
d)該付着したポリマー構築ブロックの保護基を除去し、
e)前記第一の微小液滴の上に、第一のポリマー構築ブロックと同じ、あるいはそれとは異なる、第二のポリマー構築ブロックからなる第二の微小液滴をアプライし、
f)前記第の二ポリマー構築ブロックを、第一のポリマー構築ブロックの脱保護した反応基と反応させ、
g)必要に応じて手順d)−f)を連続して繰り返す、
という手順からなる方法であり、手順c)の後でポリマー表面の未反応の官能基が、化学的に不活性の化学種に変換されることを特徴とする方法。 - 第一の微小液滴の直径が、1μmと1000μmの間であることを特徴とする、請求項10に記載の方法。
- 第一の微小液滴の直径が、第二の微小液滴の直径より小さいことを特徴とする、請求項11に記載の方法。
- 化学的に不活性な化学種がリン基を含むことを特徴とする、請求項12に記載の方法。
- ポリマー表面が、基板の不可分の一部であることを特徴とする、請求項1〜請求項13のいずれか一つに記載の方法。
- ポリマー表面が有機ポリマーからなることを特徴とする、請求項14に記載の方法。
- 反応基がOH、NRHから選ばれ、ここでRは、H、好適にはC1−C4アルキル基、アラルキル基、シクロアルキル基であるアルキル基、およびSHであってよいことを特徴とする、請求項15に記載の方法。
- ポリマー構築ブロックが、ヌクレオシド、ヌクレオチド、アミノ酸、ペプチド、および炭水化物から選ばれることを特徴とする、請求項1〜請求項16のいずれか一つに記載の方法。
- 手順b)の直前に、活性剤を第一のポリマー構築ブロックと微小混合容器の中で混ぜ合わせることを特徴とする、請求項1〜請求項17のいずれか一つに記載の方法。
- 請求項11〜請求項18のいずれか一つに記載の方法により得ることが可能なバイオチップ。
- 表面上に反応基を有する固体基板と、表面上の反応基の一部分に付着した生体分子とからなり、表面の残りの反応基が化学的に不活性な化学種に変換されているバイオチップ。
- 化学的に不活性な化学種がリン基を含むことを特徴とする、請求項20に記載のバイオチップ。
- 生体分子が、ヌクレオチド、オリゴヌクレオチド、ポリペプチド、およびポリ炭水化物からなるグループから選ばれることを特徴とする、請求項20または請求項21に記載のバイオチップ。
- 基板が有機ポリマー物質からなることを特徴とする、請求項1〜請求項22のいずれか一つに記載のバイオチップ。
- 有機ポリマー物質がポリプロピレンであることを特徴とする、請求項24に記載のバイオチップ。
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DE2003108931 DE10308931A1 (de) | 2003-02-28 | 2003-02-28 | System und Verfahren zur Synthese von Polymeren |
PCT/EP2004/002040 WO2004076059A2 (en) | 2003-02-28 | 2004-03-01 | System and process for the synthesis of polymers |
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Publication number | Priority date | Publication date | Assignee | Title |
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US20040185169A1 (en) * | 2003-03-21 | 2004-09-23 | Peck Bill J. | Methods for fabricating biopolymer arrays |
DE102004024364A1 (de) * | 2004-05-17 | 2005-12-15 | Apibio Sas | Verfahren zur Herstellung von Polymeren |
US20060165895A1 (en) * | 2005-01-24 | 2006-07-27 | Julio Cartagena | System and a method for synthesizing nanoparticle arrays in-situ |
EP2066437A2 (en) * | 2006-09-21 | 2009-06-10 | Koninklijke Philips Electronics N.V. | Ink jet device and method for producing a biological assay substrate by releasing a plurality of substances onto the substrate |
US8580201B2 (en) | 2010-02-22 | 2013-11-12 | Oligoco, Inc. | Wicking-based reagent-solution draining in an automated system |
WO2011102913A2 (en) * | 2010-02-22 | 2011-08-25 | Oligoco | Wicking-based reagent-solution draining in an automated system |
AU2017363146B2 (en) | 2016-11-16 | 2023-11-02 | Catalog Technologies, Inc. | Systems for nucleic acid-based data storage |
US10650312B2 (en) | 2016-11-16 | 2020-05-12 | Catalog Technologies, Inc. | Nucleic acid-based data storage |
CA3094077A1 (en) | 2018-03-16 | 2019-09-19 | Catalog Technologies, Inc. | Chemical methods for nucleic acid-based data storage |
US20200193301A1 (en) | 2018-05-16 | 2020-06-18 | Catalog Technologies, Inc. | Compositions and methods for nucleic acid-based data storage |
AU2020268440A1 (en) | 2019-05-09 | 2021-12-02 | Catalog Technologies, Inc. | Data structures and operations for searching, computing, and indexing in DNA-based data storage |
JP2022551186A (ja) | 2019-10-11 | 2022-12-07 | カタログ テクノロジーズ, インコーポレイテッド | 核酸セキュリティーおよび認証 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000035940A2 (de) * | 1998-12-14 | 2000-06-22 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Verfahren und vorrichtungen zum aufbringen von substanzen auf einen träger, insbesondere von monomeren für die kombinatorische synthese von molekülbibliotheken |
JP2001518086A (ja) * | 1997-03-20 | 2001-10-09 | ユニバーシティ オブ ワシントン | バイオポリマー合成のための溶媒、溶媒微小液滴、および使用方法 |
JP2003219872A (ja) * | 2002-01-30 | 2003-08-05 | Takahiro Sato | ヌクレオチドをインクジェットプリンティング方式で並べdnaやrnaまたタンパク質を自在に配列する装置。 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB874665A (en) * | 1959-01-26 | 1961-08-10 | Metal Box Co Ltd | Improvements in or relating to apparatus for setting-up cartons |
US5508200A (en) * | 1992-10-19 | 1996-04-16 | Tiffany; Thomas | Method and apparatus for conducting multiple chemical assays |
US6015880A (en) * | 1994-03-16 | 2000-01-18 | California Institute Of Technology | Method and substrate for performing multiple sequential reactions on a matrix |
US5847674A (en) * | 1996-05-02 | 1998-12-08 | Moore Business Forms, Inc. | Apparatus and methods for maintaining optimum print quality in an ink jet printer after periods of inactivity |
US6461812B2 (en) * | 1998-09-09 | 2002-10-08 | Agilent Technologies, Inc. | Method and multiple reservoir apparatus for fabrication of biomolecular arrays |
US6309828B1 (en) * | 1998-11-18 | 2001-10-30 | Agilent Technologies, Inc. | Method and apparatus for fabricating replicate arrays of nucleic acid molecules |
FR2790421A1 (fr) * | 1999-03-01 | 2000-09-08 | Gemplus Card Int | Machine d'impression graphique pour support de memorisation de type carte, procede d'impression graphique desdits supports de memorisation et supports de memorisation |
US6171797B1 (en) * | 1999-10-20 | 2001-01-09 | Agilent Technologies Inc. | Methods of making polymeric arrays |
US6649348B2 (en) * | 2001-06-29 | 2003-11-18 | Agilent Technologies Inc. | Methods for manufacturing arrays |
-
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001518086A (ja) * | 1997-03-20 | 2001-10-09 | ユニバーシティ オブ ワシントン | バイオポリマー合成のための溶媒、溶媒微小液滴、および使用方法 |
WO2000035940A2 (de) * | 1998-12-14 | 2000-06-22 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Verfahren und vorrichtungen zum aufbringen von substanzen auf einen träger, insbesondere von monomeren für die kombinatorische synthese von molekülbibliotheken |
JP2003516308A (ja) * | 1998-12-14 | 2003-05-13 | ドイチェス クレープスフォルシュングスツェントルム シュティフトゥング デス エッフェントリッヒェン レヒツ | 分子ライブラリーをコンビナトリアル合成するための物質、とりわけモノマーを担体に付着させる方法と装置 |
JP2003219872A (ja) * | 2002-01-30 | 2003-08-05 | Takahiro Sato | ヌクレオチドをインクジェットプリンティング方式で並べdnaやrnaまたタンパク質を自在に配列する装置。 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2021522806A (ja) * | 2018-05-09 | 2021-09-02 | ヴィブラント ホールディングス リミテッド ライアビリティ カンパニー | 光活性化された作用物質を使用してポリヌクレオチドアレイを合成する方法 |
JP7422087B2 (ja) | 2018-05-09 | 2024-01-25 | ヴィブラント ホールディングス リミテッド ライアビリティ カンパニー | 光活性化された作用物質を使用してポリヌクレオチドアレイを合成する方法 |
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