JP2006143816A - Manufacturing method of decolored fucoidan - Google Patents
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- JP2006143816A JP2006143816A JP2004333404A JP2004333404A JP2006143816A JP 2006143816 A JP2006143816 A JP 2006143816A JP 2004333404 A JP2004333404 A JP 2004333404A JP 2004333404 A JP2004333404 A JP 2004333404A JP 2006143816 A JP2006143816 A JP 2006143816A
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Abstract
Description
本発明はオゾンを利用した脱色フコイダンの製造方法および当該方法により得られる脱色フコイダンに関する。 The present invention relates to a method for producing decolorized fucoidan using ozone and a decolorized fucoidan obtained by the method.
フコイダンはモズク、ワカメ、コンブ等の褐藻類に含まれるフコースを主成分とする硫酸化多糖の総称である。このフコイダンはアポトーシス誘発(特許文献1)、抗潰瘍(特許文献2)、感染症予防(特許文献3)等の作用が知られているため、薬剤、飲食品、化粧品等への利用が期待されている。 Fucoidan is a general term for sulfated polysaccharides mainly composed of fucose contained in brown algae such as mozuku, wakame, and kombu. This fucoidan is known to be effective in inducing apoptosis (Patent Document 1), anti-ulcer (Patent Document 2), infectious disease prevention (Patent Document 3), etc., and is expected to be used for drugs, foods and drinks, cosmetics, etc. ing.
しかしながら、褐藻類より抽出した状態のフコイダンは褐色であり、この色が薬剤、飲食品、化粧品等への利用の妨げとなっていた。 However, fucoidan in a state extracted from brown algae is brown, and this color has hindered its use for drugs, foods and drinks, cosmetics and the like.
これまで、フコイダンを脱色するために様々な試みがなさられていたが、フコイダンの褐色の原因が不明であるため、脱色に複雑な工程が必要であったり、脱色された場合でも脱色に用いる薬剤の残留やフコイダンの分解がなどの問題が懸念されることがあった。
従って、本発明はフコイダンを安全で且つ簡便に脱色することのできる技術を提供することを課題とする。 Therefore, an object of the present invention is to provide a technique capable of safely and easily decolorizing fucoidan.
本発明者らは上記課題を解決するために鋭意研究した結果、フコイダンを含有する溶液にオゾンを導入することにより、簡便に脱色できることおよび脱色前後でフコイダン含量に殆ど変化がないことを見出し、本発明を完成した。 As a result of intensive studies to solve the above problems, the present inventors have found that by introducing ozone into a solution containing fucoidan, it can be easily decolored and that there is almost no change in fucoidan content before and after decoloration. Completed the invention.
すなわち、本発明はフコイダンを含有する溶液に、オゾンを0.5〜20.0g/hで導入することを特徴とする脱色フコイダンの製造方法を提供するものである。 That is, the present invention provides a method for producing decolorized fucoidan, wherein ozone is introduced into a solution containing fucoidan at 0.5 to 20.0 g / h.
また、本発明は上記製造方法により得られる脱色フコイダンを提供するものである。 Moreover, this invention provides the decoloring fucoidan obtained by the said manufacturing method.
本発明の脱色フコイダンの製造方法は、オゾンを利用して脱色しているため安全であり、且つ脱色前後でフコイダン含量に殆ど変化のないフコイダンを得ることのできる優れた方法である。 The method for producing decolorized fucoidan according to the present invention is safe because decolorization is performed using ozone, and is an excellent method capable of obtaining fucoidan having almost no change in fucoidan content before and after decolorization.
また、本発明の脱色フコイダンの製造方法は、簡便な装置で行うことができるので、容易にスケールアップすることのできる工業的に優れた方法である。 Moreover, since the manufacturing method of the decolorization fucoidan of this invention can be performed with a simple apparatus, it is an industrially excellent method which can be scaled up easily.
本発明の脱色フコイダンの製造方法(以下、「本発明製法」という)は、フコイダンを含有する溶液(以下、単に「フコイダン溶液」という)を原料とし、これを脱色する方法である。 The method for producing decolorized fucoidan of the present invention (hereinafter referred to as “process of the present invention”) is a method for decolorizing a solution containing fucoidan (hereinafter simply referred to as “fucoidan solution”).
原料となるフコイダン溶液は脱色されていないフコイダン、すなわち褐色を呈し、特有の海藻臭を有するフコイダンを含有するものであれば特に制限されないが、例えば、モズク、ワカメ、コンブ等の褐藻類、好ましくはオキナワモズクから常法により調製されるフコイダンを含有する粗抽出液や、その乾燥物を水等に再溶解したもの等を用いることができる。また、このフコイダン溶液はフコイダンを0.1〜20.0質量%程度、特に0.1〜5.0質量%含有するものであることが好ましい。 The fucoidan solution used as a raw material is not particularly limited as long as it contains fucoidan that has not been decolored, i.e. brown, and contains fucoidan having a characteristic seaweed odor, for example, brown algae such as mozuku, wakame, and kombu, preferably A crude extract containing fucoidan prepared from Okinawa mozuku by a conventional method, a solution obtained by re-dissolving the dried product in water or the like can be used. The fucoidan solution preferably contains about 0.1 to 20.0% by mass of fucoidan, particularly 0.1 to 5.0% by mass.
上記のフコイダン溶液中のフコイダンを脱色するにはオゾンを0.5〜20.0g/h、好ましくは1.0〜3.0g/hで導入すればよい。オゾンの導入時間は、脱色の度合いを見て適宜選択すればよいが、5〜120分、好ましくは10〜20分程度である。また、オゾンの導入方法は特に限定されないが、例えば、フコイダン溶液中に直接吹き込むことが好ましい。 In order to decolorize fucoidan in the above fucoidan solution, ozone may be introduced at 0.5 to 20.0 g / h, preferably 1.0 to 3.0 g / h. The introduction time of ozone may be appropriately selected in view of the degree of decolorization, but is about 5 to 120 minutes, preferably about 10 to 20 minutes. The method for introducing ozone is not particularly limited, but it is preferable to blow it directly into a fucoidan solution, for example.
斯くして得られる脱色フコイダンは、その1.0gを100mlの水に溶解した場合に、450nmの吸光度が0.15以下、好ましくは0.10以下で、520nmの吸光度が0.05以下、好ましくは0.03以下のものである。また、脱色前後でフコイダン含量に変化が認められないので、脱色による基本構造の変化はないものと考えられる。 When 1.0 g of the decolorized fucoidan thus obtained is dissolved in 100 ml of water, the absorbance at 450 nm is 0.15 or less, preferably 0.10 or less, and the absorbance at 520 nm is 0.05 or less. Is 0.03 or less. In addition, since no change in the fucoidan content was observed before and after decolorization, it is considered that there was no change in the basic structure due to decolorization.
また、本発明製法には、電気透析、限外濾過等による不純物除去、噴霧乾燥、凍結乾燥等による乾燥、精密濾過等による無菌濾過、超高温殺菌等による殺菌等の工程を追加することができる。 Further, the method of the present invention can be added with steps such as impurity removal by electrodialysis, ultrafiltration, etc., drying by spray drying, freeze drying, etc., aseptic filtration by microfiltration, etc., sterilization by ultra-high temperature sterilization, etc. .
このようにして得られるフコイダンは、脱色されているため、着色を嫌う各種の組成物、例えば、薬剤、飲食品、化粧品等の様々な分野への利用が可能である。 Since the fucoidan thus obtained is decolorized, it can be used in various fields such as various compositions that dislike coloring, such as drugs, foods and drinks, and cosmetics.
参 考 例 1
オキナワモズク抽出液の調製:
オキナワモズクの50kgを50lの水に浸漬し、95℃で60分間加熱攪拌抽出を行い粗抽出液を得た。次いで、この粗抽出液から、遠心分離により固液を分離し、その上清をオキナワモズク抽出液とした。このオキナワモズク抽出液はフコイダンを1.8質量%含有するものであり、その外観色は褐色であった。
Reference example 1
Preparation of Okinawa mozuku extract:
50 kg of Okinawa mozuku was soaked in 50 l of water and subjected to extraction with heating at 95 ° C. for 60 minutes to obtain a crude extract. Next, solid liquid was separated from this crude extract by centrifugation, and the supernatant was used as Okinawa mozuku extract. This Okinawa mozuku extract contained 1.8% by mass of fucoidan, and its appearance color was brown.
実 施 例 1
脱色フコイダンの製造(1):
参考例1で調製したオキナワモズク抽出液500mlに、オゾン処理装置(ON-3-2:日本オゾン(株)製)を用いてオゾンを2g/hで導入した。オゾン導入後0、1、2.5、5、7.5、10分の520nmおよび450nmの吸光度を分光光度計(V-350型:日本分光(株)製)で測定した。また、15分後の脱色率を下記の式により求めた。
Example 1
Manufacture of decolorized fucoidan (1):
To 500 ml of Okinawa mozuku extract prepared in Reference Example 1, ozone was introduced at 2 g / h using an ozone treatment device (ON-3-2: manufactured by Nippon Ozone Co., Ltd.). Absorbance at 520 nm and 450 nm at 0, 1, 2.5, 5, 7.5, 10 minutes after introduction of ozone was measured with a spectrophotometer (V-350 type: manufactured by JASCO Corporation). Further, the decolorization rate after 15 minutes was determined by the following formula.
(式1)
脱色率(%)=100−((脱色後の吸光度/脱色前の吸光度)×100)
(Formula 1)
Decolorization rate (%) = 100 − ((absorbance after decolorization / absorbance before decolorization) × 100)
オゾン導入開始から、15分で約90%の脱色率が得られた。また、さらにオゾンの導入を続けるとほぼ無色にまで脱色することができた。 A decolorization rate of about 90% was obtained in 15 minutes from the start of ozone introduction. Moreover, when ozone was further introduced, it was possible to decolorize to almost colorless.
実 施 例 2
脱色フコイダンの製造(2):
フコイダン((株)サウスプロダクト製)を0.1%、0.5%、1.0%含有する水溶液を調製し、これらに実施例1と同様にオゾンを2g/hで導入した。実施例1と同様に吸光度を測定し、脱色率を求めた。
Example 2
Production of decolorized fucoidan (2):
Aqueous solutions containing 0.1%, 0.5%, and 1.0% fucoidan (manufactured by South Product Co., Ltd.) were prepared, and ozone was introduced at 2 g / h in the same manner as in Example 1. The absorbance was measured in the same manner as in Example 1 to determine the decolorization rate.
何れの濃度のフコイダン含有溶液においても褐色の溶液が、淡黄色もしくはほとんど無色となった。 In any concentration of fucoidan-containing solution, the brown solution became pale yellow or almost colorless.
実 施 例 3
脱色フコイダンの製造(3):
フコイダン((株)サウスプロダクト製)を5%含有する水溶液を調製し、これに実施例1と同様にオゾンを2g/hまたは3g/hで導入した。実施例1と同様に吸光度を測定し、脱色率を求めた。
Example 3
Production of decolorized fucoidan (3):
An aqueous solution containing 5% fucoidan (manufactured by South Product Co., Ltd.) was prepared, and ozone was introduced thereto at 2 g / h or 3 g / h in the same manner as in Example 1. The absorbance was measured in the same manner as in Example 1 to determine the decolorization rate.
フコイダンを5%含有する溶液については、何れのオゾン濃度でも時間経過と共に褐色の溶液が淡黄色に脱色され、脱色率はオゾン供給濃度が2g/hの場合20分で、3g/hでは15分で90%以上の脱色率となり、効率よく脱色されていることが確認された。 For solutions containing 5% fucoidan, the brown solution is decolorized to light yellow over time at any ozone concentration, and the decolorization rate is 20 minutes when the ozone supply concentration is 2 g / h, and 15 minutes at 3 g / h. The decolorization rate was 90% or more, and it was confirmed that the color was efficiently decolored.
実 施 例 4
脱色フコイダンの製造(4):
フコイダン((株)サウスプロダクト製)を5%含有する水溶液を調製し、これに実施例1と同様にオゾンを2g/hで導入した。実施例1と同様に吸光度を測定し、脱色率を求めた。また、脱色前後のフコイダン回収率をアンスロン硫酸法により求めた。
Example 4
Production of decolorized fucoidan (4):
An aqueous solution containing 5% of fucoidan (manufactured by South Product Co., Ltd.) was prepared, and ozone was introduced thereto at 2 g / h as in Example 1. The absorbance was measured in the same manner as in Example 1 to determine the decolorization rate. Further, the fucoidan recovery rate before and after decolorization was determined by the anthrone sulfuric acid method.
脱色の間もフコイダンの含量はほとんど変化がなかった。 There was little change in fucoidan content during decolorization.
実 施 例 5
脱色フコイダンの製造(5):
フコイダン((株)サウスプロダクト製)を5%含有する水溶液450mlに、オゾンを1g/hで30分間導入し、脱色標品を調製した。本標品の脱色率は85%であり、フコイダン回収率(%)は99.7%であった。次いで、この溶液に凍結乾燥処理を行い、海藻臭が除去され、淡黄色の脱色フコイダン粉末17.3gを得た。
Example 5
Production of decolorized fucoidan (5):
Ozone was introduced into 450 ml of an aqueous solution containing 5% fucoidan (manufactured by South Product Co., Ltd.) at 1 g / h for 30 minutes to prepare a decolorized sample. The color removal rate of this sample was 85%, and the fucoidan recovery rate (%) was 99.7%. Next, this solution was freeze-dried to remove seaweed odor, and 17.3 g of pale yellow decolorized fucoidan powder was obtained.
本発明の脱色フコイダンの製造方法によれば、脱色されたフコイダンを簡便に得ることができる。 According to the method for producing a decolorized fucoidan of the present invention, a decolorized fucoidan can be easily obtained.
そして、上記方法により得られる脱色フコイダンは、従来の褐色のフコイダンと比較して、食品・飲料等に添加した場合に色の変化を抑えることができ、しかも、脱色前後でフコイダン含量に殆ど変化がないため、各種飲食品、化粧品、薬剤等の素材としての有利に使用することができるものであり、フコイダンの用途が広がり、フコイダン市場の拡大に繋がることが期待される。
以 上
The decolorized fucoidan obtained by the above method can suppress the color change when added to foods and beverages, etc., compared to the conventional brown fucoidan, and there is almost no change in the fucoidan content before and after decolorization. Therefore, it can be advantageously used as a raw material for various foods, cosmetics, medicines, etc., and the use of fucoidan is expected to expand, leading to the expansion of the fucoidan market.
more than
Claims (4)
Decolorized fucoidan obtained by the production method according to any one of claims 1 to 3.
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Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04293901A (en) * | 1991-03-22 | 1992-10-19 | Shin Etsu Chem Co Ltd | Production of cellulose derivative of improved whiteness |
JPH067200A (en) * | 1992-02-07 | 1994-01-18 | Natl Starch & Chem Investment Holding Corp | Refining of polysaccharide |
JPH07138166A (en) * | 1993-09-24 | 1995-05-30 | Yakult Honsha Co Ltd | Anti-tumor agent and anchoring inhibitor against helicobacter pyrolii |
WO1997047208A1 (en) * | 1996-06-12 | 1997-12-18 | Takara Shuzo Co., Ltd. | Foods or drinks |
JPH10191940A (en) * | 1997-01-16 | 1998-07-28 | Yakult Honsha Co Ltd | Improvement of quality of mozuku extract and mozuku extract |
JPH10237103A (en) * | 1996-12-24 | 1998-09-08 | Masakuni Tako | Acetylfucoidan obtained using cultured okinawa-mozuku as raw material and its production |
JPH1180003A (en) * | 1997-09-16 | 1999-03-23 | Yakult Honsha Co Ltd | Agent for preventing and treating infectious disease |
JP2000351801A (en) * | 1999-06-14 | 2000-12-19 | Yakult Honsha Co Ltd | Highly pure fucoidan and preparation thereof |
-
2004
- 2004-11-17 JP JP2004333404A patent/JP2006143816A/en active Pending
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04293901A (en) * | 1991-03-22 | 1992-10-19 | Shin Etsu Chem Co Ltd | Production of cellulose derivative of improved whiteness |
JPH067200A (en) * | 1992-02-07 | 1994-01-18 | Natl Starch & Chem Investment Holding Corp | Refining of polysaccharide |
JPH07138166A (en) * | 1993-09-24 | 1995-05-30 | Yakult Honsha Co Ltd | Anti-tumor agent and anchoring inhibitor against helicobacter pyrolii |
WO1997047208A1 (en) * | 1996-06-12 | 1997-12-18 | Takara Shuzo Co., Ltd. | Foods or drinks |
JPH10237103A (en) * | 1996-12-24 | 1998-09-08 | Masakuni Tako | Acetylfucoidan obtained using cultured okinawa-mozuku as raw material and its production |
JPH10191940A (en) * | 1997-01-16 | 1998-07-28 | Yakult Honsha Co Ltd | Improvement of quality of mozuku extract and mozuku extract |
JPH1180003A (en) * | 1997-09-16 | 1999-03-23 | Yakult Honsha Co Ltd | Agent for preventing and treating infectious disease |
JP2000351801A (en) * | 1999-06-14 | 2000-12-19 | Yakult Honsha Co Ltd | Highly pure fucoidan and preparation thereof |
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