JP2005082500A - Hair grower - Google Patents
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- JP2005082500A JP2005082500A JP2003313564A JP2003313564A JP2005082500A JP 2005082500 A JP2005082500 A JP 2005082500A JP 2003313564 A JP2003313564 A JP 2003313564A JP 2003313564 A JP2003313564 A JP 2003313564A JP 2005082500 A JP2005082500 A JP 2005082500A
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Abstract
Description
本発明は、育毛促進及び脱毛防止を行ううえで、有用な作用を発揮し得る育毛剤に関するものである。 The present invention relates to a hair restorer that can exhibit a useful effect in promoting hair growth and preventing hair loss.
育毛促進及び脱毛防止の育毛剤については、色々な素材が提供されており、出願人自身、これまで各種柑橘類の果皮およびアロエの日本酒による抽出液の混合液による構成(特開平3−227911)、柑橘類に由来し、所定の物理的化学的性質を有するペプチドによる構成(特開平9−202716)を提唱したことがある。 Various materials are provided for the hair growth-promoting agent for preventing hair growth and preventing hair loss. The applicant himself has so far constituted a mixture of various citrus peels and an extract of aloe sake (Japanese Patent Laid-Open No. 3-227911), A structure derived from citrus fruits and having a predetermined physical and chemical property (JP 9-202716 A) has been proposed.
各素材と育毛促進、又は脱毛防止に関する具体的なプロセスに関する体内の生化学上の詳細なメカニズムは、必ずしも解明されている訳ではないが、これまでアミノ酸の単体を基本構成とする育毛作用の開発は、これまで行われていなかった。 The detailed biochemical mechanism in the body related to each material and the specific process related to hair growth promotion or hair loss prevention has not necessarily been elucidated. Has never been done before.
本発明は、アミノ酸を構成上の基本単位とする育毛剤を提供することを課題とするものである。 An object of the present invention is to provide a hair restorer having an amino acid as a structural basic unit.
前記課題を解決するため、本発明の構成は、L−アスパラギンを含有する育毛剤からなる。 In order to solve the said subject, the structure of this invention consists of a hair restorer containing L-asparagine.
上記のように、本発明は、L−アスパラギンを育毛剤の用途として使用することにより、育毛促進及び脱毛防止などの育毛作用を効率的に実現することができる。 As mentioned above, this invention can implement | achieve hair-growth effect | actions, such as hair growth promotion and hair loss prevention, efficiently by using L-asparagine as a use of a hair-growth agent.
本発明は、単一のアミノ酸による基本構成として、L−アスパラギンを採用しているが、育毛効果を発揮するための最良の形態は、L−アスパラギンの水溶液である。 Although the present invention employs L-asparagine as a basic composition of a single amino acid, the best mode for exhibiting the hair-growth effect is an aqueous solution of L-asparagine.
L−アスパラギンの前記育毛作用は、所謂毛乳頭細胞増殖促進作用を介して確認することができるが、この点に関するL−アスパラギンの水溶液を使用したことによる試験の経過、及び結果は、以下の通りである。 The hair growth effect of L-asparagine can be confirmed through a so-called dermal papilla cell growth promoting effect. The progress and results of the test using an aqueous solution of L-asparagine in this regard are as follows. It is.
1)培養毛乳頭細胞の継代培養及び保存
最初の培養において、十分な細胞増殖が得られた時点(2〜3週間後)でカバーグラスを外し、0.25%トリプシン−0.02% EDTA(エチレンジアミン四酢酸)溶液処理により細胞を剥離し、10%FBS−DMEM培地で1〜2週間毎継代培養した。
1) Subculture and storage of cultured dermal papilla cells In the initial culture, when sufficient cell growth was obtained (after 2 to 3 weeks), the cover glass was removed, and 0.25% trypsin-0.02% EDTA. Cells were detached by treatment with (ethylenediaminetetraacetic acid) solution and subcultured every 10 to 2 weeks in 10% FBS-DMEM medium.
更に、培養毛乳頭細胞を10%DMSO−DMEM培地に懸濁し、−85℃にて凍結保存した。以下の試験には、凍結保存より解凍した培養毛乳頭細胞を継代培養し、継代3〜7代の培養毛乳頭細胞を使用した。 Furthermore, the cultured hair papilla cells were suspended in 10% DMSO-DMEM medium and stored frozen at -85 ° C. In the following tests, cultured hair papilla cells thawed from cryopreservation were subcultured, and cultured hair papilla cells from passage 3 to 7 were used.
2)培養毛乳頭細胞の増殖促進効果の測定の準備
継代培養により十分に増殖した培養毛乳頭細胞を0.25%トリプシン−0.02% EDTA(エチレンジアミン四酢酸)溶液処理し、10%FBS−DMEM培地にて均一な細胞浮遊液(1.5〜2×104 cells/ml)に調整した。
2) Preparation for measurement of growth promoting effect of cultured hair papilla cells Cultured hair papilla cells sufficiently grown by subculture were treated with 0.25% trypsin-0.02% EDTA (ethylenediaminetetraacetic acid) solution and 10% FBS. -It adjusted to the uniform cell suspension (1.5-2 * 10 < 4 > cells / ml) with the DMEM culture medium.
この細胞浮遊液を96培養穴のマルチプレートに培養穴あたり180〜195μl植え込み、L−アスパラギンの水溶液を5〜10μl/培養穴添加し、混和した後、炭酸ガス(CO2)5%、37℃の条件下で3日間培養した後、培地を取り除き、新鮮な10%FBS−DMEM培地を培養穴あたり180〜195μl、L−アスパラギンの水溶液及びその希釈液を5〜10μl/培養穴添加し、混和した後、炭酸ガス(CO2)5%、37℃の条件下で引き続き3日間培養した(2回添加:6日間培養)。 This cell suspension was planted in a 96-well multi-plate at 180 to 195 μl per culture hole, and 5 to 10 μl / culture hole of an L-asparagine aqueous solution was added and mixed, followed by carbon dioxide (CO 2 ) 5%, 37 ° C. After culturing for 3 days under the above conditions, the medium was removed, fresh 10% FBS-DMEM medium was added at 180 to 195 μl per culture well, an aqueous solution of L-asparagine and its diluted solution was added at 5 to 10 μl / culture hole, and mixed. After that, the cells were continuously cultured for 3 days under conditions of 5% carbon dioxide (CO 2 ) and 37 ° C. (added twice: cultured for 6 days).
3)測定結果
細胞の増殖の有無は、MTT assay法による測定を行った。
前記測定によるウサギ髭培養毛乳頭細胞に対する作用の結果は、以下の表1、及び図1に示す通りである。
表1
3) Measurement results The presence or absence of cell proliferation was measured by the MTT assay method.
The result of the effect | action with respect to the rabbit rabbit cultured hair papilla cell by the said measurement is as showing in the following Table 1 and FIG.
Table 1
前記試験結果によれば、L−アスパラギンの水溶液によって、その濃度が約0.3μg/mlの場合において既に毛乳頭細胞増殖促進作用が認められ、前記濃度が、1.25(μg/ml)に至った場合には、約120%の毛乳頭細胞増殖促進作用を有することが判明した。 According to the test results, the L-asparagine aqueous solution has already been shown to promote hair papilla cell proliferation promoting action when the concentration is about 0.3 μg / ml, and the concentration is 1.25 (μg / ml). In the case of reaching, it has been found that the hair papilla cell proliferation promoting action is about 120%.
このように、アミノ酸であるL−アスパラギン酸の単体が、相当程度の育毛作用を有しており、L−アスパラギン酸を育毛剤として使用し得ることが明らかとなった。 Thus, it became clear that the simple substance of L-aspartic acid which is an amino acid has a considerable hair-restoring action, and L-aspartic acid can be used as a hair-restoring agent.
甘夏カン果皮エキスに対し、活性炭カラムクロマトグラフィー、セルロースカラムクロマトグラフィー、HPLC(高速度液体クロマトグラフィー)による各成分の抽出、及び濃縮工程を繰り返し、最終的に得られた白色粉末をNMRによって検査したところ、L−アスパラギンが検出された。 Repeated extraction and concentration steps of activated carbon column chromatography, cellulose column chromatography, HPLC (high-speed liquid chromatography), and concentration process for the sweet summer pericarp extract, and finally obtained white powder was examined by NMR As a result, L-asparagine was detected.
但し、前記白色粉末を得るに至るHPLCの過程、及び前記白色粉末の直前の原材料物質として、同じように白色粉末状を呈している素材に対する赤外線スペクトル等の検査結果によれば、前記白色粉末は、単にL−アスパラギンの単量体だけでなく、二量体及び三量体等の多量体を構成し、L−アスパラギンが相互に結合したことによるペプチドをも含んでいる可能性が極めて強いことが明らかとなった。 However, according to the results of the inspection of the infrared spectrum and the like for the raw material substance just before the white powder as the raw material substance just before the white powder, the white powder, It is very likely that not only L-asparagine monomers but also multimers such as dimers and trimers, and peptides containing L-asparagine bound to each other are included. Became clear.
上記白色物質について、L−アスパラギンと全く同様の試験を行ったところ、下記表2、及び図1に示す結果を得ることができた。
表2
When the white substance was tested in exactly the same manner as L-asparagine, the results shown in Table 2 below and FIG. 1 could be obtained.
Table 2
上記表1と表2との対比、及び図1の表示からも明らかなように、L−アスパラギンの単体のみならず、L−アスパラギン同士の結合によるペプチドと共存している育毛剤の場合には、L−アスパラギンと略同等又はそれ以上の毛乳頭細胞増殖促進効果を有していることが判明した。 As is clear from the comparison between Table 1 and Table 2 and the display of FIG. 1, in the case of a hair restorer coexisting not only with L-asparagine alone but also with a peptide due to the binding of L-asparagine. , L-asparagine was found to have hair dermal papilla cell proliferation promoting effects substantially equivalent to or higher than those of L-asparagine.
出願人が提唱した特開平9−202716号による構成は、前記白色粉末に至るために、HPLCによる抽出を行う前段階の素材に対する赤外線分析、及び加水分解を行った後のアミノ酸分析に由来している。 The configuration according to Japanese Patent Laid-Open No. 9-202716 proposed by the applicant is derived from infrared analysis of the material before the extraction by HPLC and amino acid analysis after hydrolysis to reach the white powder. Yes.
そして、前記白色粉末を最終的なHPLCによって生成する際に、相当量のペプチドを除去していること、及び前記白色粉末に対するNMR検査において、L−アスパラギン以外のペプチドが存在していることが判明していることを考慮するならば、前記特開平9−202716号に係る構成と、前記実施例の構成とは、共通する原材料に基づく素材において、異なる構成成分に着目した関係にあるものと解することが出来る。
And when producing | generating the said white powder by final HPLC, it turned out that a considerable amount of peptides are removed, and the peptide other than L-asparagine exists in the NMR test | inspection with respect to the said white powder. In view of this, it is understood that the configuration according to the above-mentioned Japanese Patent Laid-Open No. 9-202716 and the configuration of the above example are in a relationship based on different components in materials based on common raw materials. I can do it.
本発明は、典型的なアミノ酸であるL−アスパラギンを育毛剤として使用するという新たな産業上の利用可能性を提供している。 The present invention provides a new industrial applicability of using L-asparagine, a typical amino acid, as a hair restorer.
Claims (4)
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JP2003313564A JP2005082500A (en) | 2003-09-05 | 2003-09-05 | Hair grower |
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JP2003313564A JP2005082500A (en) | 2003-09-05 | 2003-09-05 | Hair grower |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2939038A1 (en) * | 2008-12-02 | 2010-06-04 | Oreal | Oral or topical cosmetic use of combination of glutamic acid, threonine, asparagine and alanine, to prevent and/or treat hair loss and/or alopecia in men |
FR2939029A1 (en) * | 2008-12-02 | 2010-06-04 | Oreal | Oral or topical cosmetic use of combination of reduced glutathione or its analogs or antioxidant precursors, glutamine, leucine, asparagine and tyrosine, to prevent and/or treat hair loss and/or alopecia in women |
WO2010064203A1 (en) * | 2008-12-02 | 2010-06-10 | L'oreal | Combination of reduced glutathione and amino acids for improving the quality of the hair in women |
Citations (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH03227911A (en) * | 1990-01-31 | 1991-10-08 | Yutaka Miyauchi | Hair-growing agent |
JPH04290813A (en) * | 1990-12-07 | 1992-10-15 | Unilever Nv | Cosmetic composition |
JPH05286834A (en) * | 1992-04-03 | 1993-11-02 | Kanebo Ltd | Hair-raising agent |
JPH06128125A (en) * | 1992-10-14 | 1994-05-10 | Kose Corp | Hair tonic |
DE4401274A1 (en) * | 1994-01-18 | 1995-07-20 | Rooyan Behdasht Co | Liq. agent for promoting and maintaining hair growth |
JPH08337563A (en) * | 1995-04-11 | 1996-12-24 | Kashima Sekiyu Kk | N-acylamino acid and its salt |
JPH09202716A (en) * | 1996-01-25 | 1997-08-05 | Yutaka Miyauchi | Hair tonic |
JPH09241131A (en) * | 1996-03-01 | 1997-09-16 | Nendo Kagaku Kenkyusho:Kk | Hair tonic |
JPH09278629A (en) * | 1996-04-12 | 1997-10-28 | Yamanashi Kk | Growth-stimulating agent for hair |
JPH11228352A (en) * | 1998-02-19 | 1999-08-24 | Kanebo Ltd | Hair tonic and hair treatment |
JP2000290146A (en) * | 1999-04-02 | 2000-10-17 | Yoshiharu Harimoto | Solution obtained by extracting active ingredient contained in citrus unshu marc. with organic solvent using carrier oil as solvent liquid or hair growth agent and skin care oil with carrier oil containing the same |
JP2002029935A (en) * | 2000-07-17 | 2002-01-29 | Taisho Pharmaceut Co Ltd | Hair-growing agent |
JP2002212034A (en) * | 2001-01-16 | 2002-07-31 | Fancl Corp | Hair cosmetic |
JP2002363035A (en) * | 2001-04-06 | 2002-12-18 | Nakahara Kimiko | Hair grower |
JP2004035531A (en) * | 2002-07-05 | 2004-02-05 | Nobuyo Azuma | Hair grower and cosmetic containing the same |
-
2003
- 2003-09-05 JP JP2003313564A patent/JP2005082500A/en active Pending
Patent Citations (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH03227911A (en) * | 1990-01-31 | 1991-10-08 | Yutaka Miyauchi | Hair-growing agent |
JPH04290813A (en) * | 1990-12-07 | 1992-10-15 | Unilever Nv | Cosmetic composition |
JPH05286834A (en) * | 1992-04-03 | 1993-11-02 | Kanebo Ltd | Hair-raising agent |
JPH06128125A (en) * | 1992-10-14 | 1994-05-10 | Kose Corp | Hair tonic |
DE4401274A1 (en) * | 1994-01-18 | 1995-07-20 | Rooyan Behdasht Co | Liq. agent for promoting and maintaining hair growth |
JPH08337563A (en) * | 1995-04-11 | 1996-12-24 | Kashima Sekiyu Kk | N-acylamino acid and its salt |
JPH09202716A (en) * | 1996-01-25 | 1997-08-05 | Yutaka Miyauchi | Hair tonic |
JPH09241131A (en) * | 1996-03-01 | 1997-09-16 | Nendo Kagaku Kenkyusho:Kk | Hair tonic |
JPH09278629A (en) * | 1996-04-12 | 1997-10-28 | Yamanashi Kk | Growth-stimulating agent for hair |
JPH11228352A (en) * | 1998-02-19 | 1999-08-24 | Kanebo Ltd | Hair tonic and hair treatment |
JP2000290146A (en) * | 1999-04-02 | 2000-10-17 | Yoshiharu Harimoto | Solution obtained by extracting active ingredient contained in citrus unshu marc. with organic solvent using carrier oil as solvent liquid or hair growth agent and skin care oil with carrier oil containing the same |
JP2002029935A (en) * | 2000-07-17 | 2002-01-29 | Taisho Pharmaceut Co Ltd | Hair-growing agent |
JP2002212034A (en) * | 2001-01-16 | 2002-07-31 | Fancl Corp | Hair cosmetic |
JP2002363035A (en) * | 2001-04-06 | 2002-12-18 | Nakahara Kimiko | Hair grower |
JP2004035531A (en) * | 2002-07-05 | 2004-02-05 | Nobuyo Azuma | Hair grower and cosmetic containing the same |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2939038A1 (en) * | 2008-12-02 | 2010-06-04 | Oreal | Oral or topical cosmetic use of combination of glutamic acid, threonine, asparagine and alanine, to prevent and/or treat hair loss and/or alopecia in men |
FR2939029A1 (en) * | 2008-12-02 | 2010-06-04 | Oreal | Oral or topical cosmetic use of combination of reduced glutathione or its analogs or antioxidant precursors, glutamine, leucine, asparagine and tyrosine, to prevent and/or treat hair loss and/or alopecia in women |
WO2010064203A1 (en) * | 2008-12-02 | 2010-06-10 | L'oreal | Combination of reduced glutathione and amino acids for improving the quality of the hair in women |
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