JP2004073964A - Colloidal gold solution and method of producing the same - Google Patents
Colloidal gold solution and method of producing the same Download PDFInfo
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【０００１】
【発明の属する技術分野】
本発明は、保存性に優れた金コロイド溶液及びその製法に関するものである。
【０００２】
【従来の技術】
現在、金コロイド溶液は、医薬品、化粧品、食品及び塗料などの広範な分野に利用されており、特に、医療用の免疫反応を利用した様々な検査用試薬の基材や抗原抗体反応などを利用したバイオセンサーの基材などとして、また 健康関連素材や美容液などにも広く使用されている。
【０００３】
従来、金コロイド溶液は、塩化金酸溶液に還元剤としてクエン酸塩溶液を加えて加熱することにより、金イオンを還元させてコロイドとする溶液内還元反応を用いて製造されている。ところが、この方法で調製された金コロイド液は、保存性が悪いために、使用する毎に調製する、いわゆる用事調製の形を採っているのが実状である。そのため、金コロイド液を使用しようとする際、その都度、金コロイド液を調製しなければならず、その調製に用いる試薬、加熱用の器具及び設備などを必要とする上に、その作業に多大な労力を要するなどの問題があった。
【０００４】
【発明が解決しようとする課題】
本発明は、従来の技術における上記した実状に鑑みてなされたものである。すなわち、本発明の目的は、金微粒子が安定した分散状態で長期に亘り保存され、簡易に繰り返し使用できる高純度の金コロイド溶液を提供することにある。また、本発明の他の目的は、金塩の還元及び得られた金微粒子の安定した分散状態の保持に好適な有機化合物を用いて、金コロイド溶液を容易に調製できる製造方法を提供することにある。
【０００５】
【課題を解決するための手段】
本発明の金コロイド溶液は、金塩を糖類で還元された金微粒子が分散している固体を、水に溶解させたことを特徴とする。
また、本発明の金コロイド溶液の製造方法は、金塩と糖類の混合溶液を加熱して蒸発乾固あるいは混合粉末を加熱融解させた後乾固させて得られた固体を、水に溶解させたことを特徴とする。上記の糖類としては、単糖類、二糖類及び多糖類から選ばれる１種以上を用いることが好ましい。また、金塩としては、塩化金酸を用いることが好ましい。
【０００６】
【発明の実施の形態】
本発明は、金コロイド溶液を必要なときに簡単に繰り返し使用できるように、あらかじめ保存可能な金コロイド溶液の中間体として、長期に亘って保存できる固体状物を作製しておき、これを使用時に水に溶解させるのみで簡易に調製できる高純度の金コロイド溶液を提供するものであって、金塩の還元とその還元により生成する金微粒子を安定した分散状態で長期間固体として保存できる特定の有機物質として、糖類を用いるものである。
【０００７】
本発明において、原料の金塩としては、塩化金酸、その水和物や塩類、金粉や金箔等を王水に溶解させたものなどが用いられる。その金塩を糖類に添加する量は、金イオンが還元されてコロイド状になる範囲であればよく、水に溶解した状態の金コロイド溶液中における金濃度としては０．０００１〜０．１重量％の範囲が好ましい。
【０００８】
金塩の還元に用いられる糖類としては、単糖類、二糖類、多糖類から選ばれる１種以上が挙げられる。その単糖類としては、炭水化物の基本構造となっている物質であって、式 Ｃｎ（Ｈ２Ｏ）ｎ（式中、ｎは１〜１０の整数である。）で表される炭水化物からなり、オキシメチレン基（−ＣＨＯＨ−）が直鎖状に連なった同族体であって、例えば、ペントース類、ヘキソース類等が含まれ、具体的にはフルクトース等が挙げられる。また、二糖類としては、麦芽糖、セロビオース等の還元性のもの、トレハロース、蔗糖等の非還元性のものが挙げられる。さらに、多糖類としては、セルロース、デンプン、グリコーゲンなどが挙げられる。
【０００９】
これらの糖類は、通常、適宜水などと混合した溶液として用い、その溶液中に金塩を添加し溶解させて用いるが、単糖類及び二糖類等は、加熱されると糖類自体に含まれる水分により水飴状になることから、必ずしも水を添加した混合溶液として用いる必要はない。
【００１０】
次に、金塩の溶解した溶液を、加熱して水分などを蒸発により乾固させて固体状物を生成させる。その加熱条件としては、金塩の溶解液から水分などが徐々に蒸発して乾燥し固体状物が得られる温度及び時間であれば良く、例えば、６０〜１２０℃の加熱下に数分間から１２時間放置するなどによって行う。
【００１１】
得られた固体物は、蒸発乾固する過程で金イオンが還元されて、コロイド状金イオンが生成しているとともに、金粒子間に糖類などの還元剤が介在しているから、生成した金粒子同士の凝集がなく、個々の金粒子は分散し安定した状態で存在しているものと考えられる。
この固体状物は、コロイド状金粒子を長期間に亘り安定して保存しているものであり、別途調製して保存しておくと、金コロイド溶液の使用を必要とする時に適量の水に溶解させるのみで、金コロイド溶液を容易に得ることができる。
【００１２】
【実施例】
以下、本発明を実施例によりさらに具体的に説明するが、本発明はこれらの実施例によって何ら限定されるものではない。
実施例１
金０．０７ｇを王水２０ｍｌ中に入れて溶解させた後、蒸発乾固させることによって塩化金酸を得た。これに水５０ｍｌ及び蔗糖０．２ｇを加えて溶解させた後、７０℃の乾燥機中に半日間放置して加熱乾燥させたところ、暗緑色の固形物が生成した。この固形物を水５０ｍｌに溶解させて金コロイド溶液を得た。得られた金コロイド溶液の光透過スペクトル図を、図１に示す。
上記の固形物は、蒸発乾固する過程の粘稠な溶液中において、金イオンが還元されてコロイド状になっているものと解される。その際、金イオンの周囲には糖分の膜が形成されるため、金粒子同士が密着して凝集することはなく、個々の金粒子は分散し安定した状態で存在しているものと考えられる。
【００１３】
実施例２
実施例１における加熱乾燥条件である７０℃の乾燥機中に半日間放置を、８０℃の乾燥機中で１２分間に代えたこと以外は、実施例１と全く同様にして、固形物を生成させ、その固形物から金コロイド溶液を得た。得られた金コロイド溶液の光透過スペクトル図を、図２に示す。
【００１４】
実施例３
実施例１における加熱乾燥条件である７０℃の乾燥機中に半日間放置を、１２０℃の乾燥機中で３分間に代えたこと以外は、実施例１と全く同様にして、固形物を生成させ、その固形物から金コロイド溶液を得た。得られた金コロイド溶液の光透過スペクトル図を、図３に示す。
【００１５】
実施例４
澱粉０．３ｇを濃度７ｍＭの塩化金酸溶液３ｍｌと混合した溶液を、９０℃で１７時間加熱したところ、黒灰色の固形物が生成した。この固形物を水に溶かしたところ、赤紫色の金コロイドを得た。また、１００℃に１５時間加熱しても、同様に金コロイドが得られた。 得られた金コロイド溶液の光透過スペクトル図を、図４に示す。
一般に、澱粉は砂糖よりも水に難溶であるが、このように澱粉も砂糖と同じく金コロイド液を得ることができた。
なお、図１〜４において、横軸は波長であり、縦軸は吸光度であって、図によれば、金コロイド固有の吸収バンドが現れており、金コロイドが生成していることを確認できる。
実施例５
固体の砂糖と塩化金酸の混合粉末を、８０℃で１２分間加熱したところ、同じく金コロイドの前駆体を得ることができた。
【００１６】
各実施例で得られた金コロイド溶液の色は、溶解当初は淡黄色であるが、時間が経つにつれて赤味を増し、１０分経過後には赤ワインのような濃い赤色になった。この赤色液のスペクトル図は、金コロイド液の呈する特有のスペクトルであるとともに、いわゆるチンダル現象も確認されたことから、金コロイド液であることは明白である。この金コロイド溶液の色は、３０分経過すると、ほぼ一定の濃度になった。また、この金コロイド溶液は非常に安定しており、数週間から数ヶ月間ほぼ一定であった。
本発明で得られる金コロイド溶液が安定性を有する主な理由は、従来のクエン酸等を還元剤とする場合とは異なり、得られた金コロイド溶液中に金属イオンが全く含まれない点にあるものと推定される。
【００１７】
【発明の効果】
本発明によれば、金コロイドをあらかじめ水に容易に溶解する固体状態にできるから、必要な時に水に溶解させるのみで簡易に高純度の金コロイド溶液を提供できるとともに、数多くの金コロイド溶液の繰り返しの使用にも迅速に対応可能である。
また、本発明は、金微粒子の分散した安定な固体状態で保存できることは、金コロイド溶液の前駆体として長期に亘って保管できるばかりでなく、移送にも便利であり、利用価値の高いものである。
【図面の簡単な説明】
【図１】本発明における一例の金コロイド溶液の光透過スペクトル図である。
【図２】本発明における他の一例の金コロイド溶液の光透過スペクトル図である。
【図３】本発明における他の一例の金コロイド溶液の光透過スペクトル図である。
【図４】本発明における他の一例の金コロイド溶液の光透過スペクトル図である。[0001]
TECHNICAL FIELD OF THE INVENTION
TECHNICAL FIELD The present invention relates to a gold colloid solution having excellent storage stability and a method for producing the same.
[0002]
[Prior art]
At present, colloidal gold solutions are used in a wide range of fields, such as pharmaceuticals, cosmetics, foods, and paints, and in particular, use the base materials of various test reagents and antigen-antibody reactions utilizing medical immune reactions. It is widely used as a base material for biosensors, as well as health-related materials and serums.
[0003]
Conventionally, a colloidal gold solution has been produced using an in-solution reduction reaction in which a citrate solution is added as a reducing agent to a chloroauric acid solution and heated to reduce gold ions to form a colloid. However, since the colloidal gold solution prepared by this method has poor storage stability, it is actually in the form of so-called business preparation, which is prepared each time it is used. Therefore, every time a colloidal gold solution is to be used, the colloidal gold solution must be prepared, which requires reagents used for the preparation, equipment and equipment for heating, and a great deal of work. There was a problem that required a lot of labor.
[0004]
[Problems to be solved by the invention]
The present invention has been made in view of the above situation in the related art. That is, an object of the present invention is to provide a high-purity gold colloid solution in which gold fine particles are stored in a stable dispersion state for a long period of time and can be used repeatedly easily. Another object of the present invention is to provide a production method capable of easily preparing a gold colloid solution by using an organic compound suitable for reducing a gold salt and maintaining a stable dispersion state of the obtained gold fine particles. It is in.
[0005]
[Means for Solving the Problems]
The gold colloid solution of the present invention is characterized in that a solid in which gold fine particles obtained by reducing a gold salt with a saccharide are dispersed is dissolved in water.
In addition, the method for producing a colloidal gold solution of the present invention comprises dissolving a solid obtained by heating a mixed solution of a gold salt and a saccharide and evaporating it to dryness or heating and melting the mixed powder and then drying to dryness. It is characterized by having. As the above saccharide, it is preferable to use one or more selected from monosaccharides, disaccharides and polysaccharides. As the gold salt, chloroauric acid is preferably used.
[0006]
BEST MODE FOR CARRYING OUT THE INVENTION
The present invention prepares a solid material that can be stored for a long time as an intermediate of a storable gold colloid solution, and uses this as an intermediate of a storable gold colloid solution so that the colloidal gold solution can be easily and repeatedly used when necessary. It provides a high-purity colloidal gold solution that can be easily prepared by simply dissolving it in water at times, and it can reduce the gold salt and store the gold fine particles generated by the reduction as a solid in a stable dispersion state for a long period of time. Saccharides are used as the organic substances.
[0007]
In the present invention, as the gold salt as a raw material, chloroauric acid, hydrates and salts thereof, gold powder, gold foil, or the like dissolved in aqua regia is used. The amount of the gold salt added to the saccharide may be within a range in which the gold ions are reduced to a colloidal form, and the gold concentration in the gold colloid solution dissolved in water is 0.0001 to 0.1% by weight. % Is preferred.
[0008]
Examples of the saccharide used for reducing the gold salt include one or more selected from monosaccharides, disaccharides, and polysaccharides. The monosaccharide is a substance having a basic structure of a carbohydrate, and is composed of a carbohydrate represented by the formula C n (H 2 O) n (where n is an integer of 1 to 10). And oxymethylene groups (—CHOH—) in a straight chain, and include, for example, pentoses and hexoses, and specifically, fructose and the like. Examples of disaccharides include reducing ones such as maltose and cellobiose, and non-reducing ones such as trehalose and sucrose. Further, polysaccharides include cellulose, starch, glycogen and the like.
[0009]
These saccharides are usually used as a solution appropriately mixed with water or the like, and a gold salt is added to the solution to dissolve the saccharides. However, monosaccharides and disaccharides and the like contain water contained in the saccharide itself when heated. Therefore, it is not always necessary to use a mixed solution containing water.
[0010]
Next, the solution in which the gold salt is dissolved is heated to evaporate water and the like to dryness, thereby producing a solid. The heating condition may be a temperature and a time at which water or the like is gradually evaporated from the solution of the gold salt and dried to obtain a solid. For example, the heating is performed at 60 to 120 ° C. for several minutes to 12 hours. It is performed by leaving it for a while.
[0011]
In the obtained solid, gold ions are reduced in the process of evaporating to dryness, colloidal gold ions are generated, and a reducing agent such as a saccharide is interposed between the gold particles. It is considered that there is no aggregation between the particles, and the individual gold particles are present in a dispersed and stable state.
This solid material stores the colloidal gold particles stably for a long period of time, and if separately prepared and stored, can be used in an appropriate amount of water when it is necessary to use the colloidal gold solution. A colloidal gold solution can be easily obtained only by dissolving.
[0012]
【Example】
Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.
Example 1
0.07 g of gold was dissolved in 20 ml of aqua regia and then evaporated to dryness to obtain chloroauric acid. After adding and dissolving 50 ml of water and 0.2 g of sucrose into this, it was left to stand in a dryer at 70 ° C. for half a day and dried by heating, whereby a dark green solid was formed. This solid was dissolved in 50 ml of water to obtain a colloidal gold solution. FIG. 1 shows a light transmission spectrum diagram of the obtained gold colloid solution.
It is understood that the above-mentioned solids are reduced to gold ions in a viscous solution in the process of evaporating to dryness to form colloids. At that time, since a sugar film is formed around the gold ions, the gold particles do not adhere to each other and do not aggregate, and it is considered that the individual gold particles are present in a dispersed and stable state. .
[0013]
Example 2
A solid was produced in exactly the same manner as in Example 1 except that the heating and drying conditions in Example 1 were changed from leaving in a dryer at 70 ° C. for half a day to drying in an 80 ° C. dryer for 12 minutes. Then, a colloidal gold solution was obtained from the solid matter. FIG. 2 shows a light transmission spectrum diagram of the obtained gold colloid solution.
[0014]
Example 3
A solid substance was produced in exactly the same manner as in Example 1 except that the heating and drying conditions in Example 1 were changed from leaving in a dryer at 70 ° C. for half a day to drying in a dryer at 120 ° C. for 3 minutes. Then, a colloidal gold solution was obtained from the solid matter. FIG. 3 shows a light transmission spectrum diagram of the obtained gold colloid solution.
[0015]
Example 4
A solution of 0.3 g of starch mixed with 3 ml of a 7 mM chloroauric acid solution was heated at 90 ° C. for 17 hours to produce a black gray solid. When this solid was dissolved in water, a red-purple gold colloid was obtained. When heated to 100 ° C. for 15 hours, gold colloid was similarly obtained. FIG. 4 shows a light transmission spectrum diagram of the obtained gold colloid solution.
In general, starch is less soluble in water than sugar. In this way, starch can obtain a colloidal gold solution like sugar.
In FIGS. 1 to 4, the horizontal axis represents wavelength, and the vertical axis represents absorbance. According to the figures, an absorption band unique to gold colloid appears, and it can be confirmed that gold colloid is generated. .
Example 5
When the mixed powder of solid sugar and chloroauric acid was heated at 80 ° C. for 12 minutes, a precursor of colloidal gold could be obtained.
[0016]
The color of the gold colloid solution obtained in each example was pale yellow at the beginning of dissolution, but increased in red over time, and turned dark red like red wine after 10 minutes. The spectrum diagram of this red liquid is a unique spectrum exhibited by the colloidal gold solution, and a so-called Tyndall phenomenon has also been confirmed. The color of the colloidal gold solution became almost constant after 30 minutes. The gold colloid solution was also very stable and remained almost constant for several weeks to several months.
The main reason that the gold colloid solution obtained in the present invention has stability is that unlike the conventional case where citric acid or the like is used as a reducing agent, the obtained gold colloid solution contains no metal ions. It is presumed that there is.
[0017]
【The invention's effect】
According to the present invention, since the gold colloid can be made into a solid state that can be easily dissolved in water in advance, a high-purity gold colloid solution can be provided simply by dissolving it in water when necessary, and a large number of gold colloid solutions can be prepared. It can respond quickly to repeated use.
In addition, the present invention can be stored in a stable solid state in which fine gold particles are dispersed, not only can it be stored as a precursor of a gold colloid solution for a long period of time, but also can be conveniently transported and has high utility value. is there.
[Brief description of the drawings]
FIG. 1 is a light transmission spectrum diagram of an example of a gold colloid solution in the present invention.
FIG. 2 is a light transmission spectrum diagram of another example of a gold colloid solution according to the present invention.
FIG. 3 is a light transmission spectrum diagram of another example of a colloidal gold solution according to the present invention.
FIG. 4 is a light transmission spectrum diagram of another example of a colloidal gold solution according to the present invention.
Claims (6)
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| JP2007235057A (en) * | 2006-03-03 | 2007-09-13 | Ishihara Chem Co Ltd | Method for forming photochemical circuit |
| WO2008058693A1 (en) * | 2006-11-17 | 2008-05-22 | Ada Cosmetic Gmbh | Body care or beauty care product |
| WO2009131603A3 (en) * | 2008-01-09 | 2010-04-01 | Umicore Ag & Co Kg | Method for preparing dispersions of precious metal nanoparticles and for isolating such nanoparticles from said dispersions |
| US7927811B2 (en) | 2003-08-08 | 2011-04-19 | Proteus Sciences Co., Ltd. | Polypeptides having brain-localizing activity and uses thereof |
| CN104646682A (en) * | 2015-02-10 | 2015-05-27 | 上海理工大学 | Method for preparing gold nanoparticles by using sugarcane extracting solution |
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- 2002-08-13 JP JP2002235926A patent/JP4051431B2/en not_active Expired - Lifetime
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| US7927811B2 (en) | 2003-08-08 | 2011-04-19 | Proteus Sciences Co., Ltd. | Polypeptides having brain-localizing activity and uses thereof |
| WO2006013650A1 (en) * | 2004-08-06 | 2006-02-09 | Tissue Targeting Japan Inc. | Carrier for migration into cerebral neuron containing metal colloid particle |
| JP2007235057A (en) * | 2006-03-03 | 2007-09-13 | Ishihara Chem Co Ltd | Method for forming photochemical circuit |
| JP4747007B2 (en) * | 2006-03-03 | 2011-08-10 | 石原薬品株式会社 | Photochemical circuit forming method and copper solution for forming photochemical circuit |
| WO2008058693A1 (en) * | 2006-11-17 | 2008-05-22 | Ada Cosmetic Gmbh | Body care or beauty care product |
| WO2009131603A3 (en) * | 2008-01-09 | 2010-04-01 | Umicore Ag & Co Kg | Method for preparing dispersions of precious metal nanoparticles and for isolating such nanoparticles from said dispersions |
| JP2011511885A (en) * | 2008-01-09 | 2011-04-14 | ユミコア・アクチエンゲゼルシャフト・ウント・コムパニー・コマンディットゲゼルシャフト | Method for producing noble metal nanoparticle dispersion and method for isolating such nanoparticles from said dispersion |
| US8529963B2 (en) | 2008-01-09 | 2013-09-10 | Umicore Ag & Co. Kg | Method for preparing dispersions of precious metal nanoparticles and for isolating such nanoparticles from said dispersions |
| CN104646682A (en) * | 2015-02-10 | 2015-05-27 | 上海理工大学 | Method for preparing gold nanoparticles by using sugarcane extracting solution |
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