JP2003315331A - Discrimination method of honey cell - Google Patents
Discrimination method of honey cellInfo
- Publication number
- JP2003315331A JP2003315331A JP2002120616A JP2002120616A JP2003315331A JP 2003315331 A JP2003315331 A JP 2003315331A JP 2002120616 A JP2002120616 A JP 2002120616A JP 2002120616 A JP2002120616 A JP 2002120616A JP 2003315331 A JP2003315331 A JP 2003315331A
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- JP
- Japan
- Prior art keywords
- keratinocytes
- shape
- image
- skin
- differentiating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Image Analysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Image Processing (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、角質細胞の映像及
び角質細胞の形状の鑑別法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for identifying an image of keratinocytes and the shape of keratinocytes.
【０００２】[0002]
【従来の技術】化粧料を使用するにあたり、重要な事項
は適切な化粧料を選択することである。この様な適切な
化粧料の選択を行う必要条件としては、皮膚の状態或い
は特性を正しく鑑別することが挙げられる。この様な皮
膚の状態或いは特性を正しく行うための技術としては、
顔の頬などの部位より粘着テープなどを用いて、ストリ
ッピングにより角質細胞を採取し、ゲンチアナバイオレ
ット等の染色剤を用いて、角質細胞を染色し、角質細胞
の境界を明確にし、角質細胞の面積或いは体積を測定し
て、その値を指標にする技術が既に確立されている。即
ち、面積或いは体積が小さいほど、角質細胞が充分成熟
しない内に最外部に上がってきてしまっており、皮膚バ
リア機能は低く、肌の状態が悪いと鑑別され、面積が大
きいほど皮膚バリア機能が高く、肌の状態が良いと鑑別
される技術である。これに加えて、どの様な形で角質細
胞が剥離されてきているか、言い換えれば、角質細胞が
まとまっていくつか取れている重層剥離が頻繁に観察さ
れるのか、殆どの角質細胞が１個１個離れて剥離してい
るのかによって、過敏な肌なのか否かが鑑別でき、これ
らをあわせて考察することにより、肌特性が的確に鑑別
できるわけである。この様な鑑別の基礎となるのは、何
れも角質細胞の形状であり、かかる形状を正確に知るに
は、これまでは先に述べたように染色剤を用いて染色す
る工程を経なければならなかった。この工程には数日の
時間を要し、適時的な観察を販売現場で行うことは極め
て困難の伴うものであった。加えて、染色に於ける染色
剤を含む廃液の処理は環境問題を考慮する上で、大きな
課題の一つであった。言い換えれば、染色工程無しに角
質細胞の形状を的確に把握できる手段の開発が望まれて
いた。2. Description of the Related Art When using cosmetics, an important matter is to select an appropriate cosmetic. As a necessary condition for selecting such an appropriate cosmetic, it is necessary to correctly distinguish the condition or characteristics of the skin. As a technique for correctly performing such a skin condition or characteristics,
From the cheeks of the face, using adhesive tape, collect keratinocytes by stripping, stain the keratinocytes with a staining agent such as gentian violet, clarify the boundaries of the keratinocytes, Techniques for measuring the area or volume and using the value as an index have already been established. That is, as the area or volume is smaller, the keratinocytes have risen to the outermost part before they have matured sufficiently, the skin barrier function is low, and it is distinguished that the skin condition is poor. It is a technology that is distinguished as being high and in good skin condition. In addition to this, how the keratinocytes have been exfoliated, in other words, whether multiple exfoliation of keratin cells that are collected together is frequently observed, most keratinocytes Whether or not the skin is hypersensitive can be discriminated by whether the skin is separated and peeled off. By considering these together, the skin characteristics can be discriminated accurately. The basis of such discrimination is the shape of keratinocytes, and in order to know such shape accurately, it is necessary to go through the step of staining with a staining agent as described above until now. did not become. This process took several days, and it was extremely difficult to perform timely observation at the sales site. In addition, the treatment of a waste liquid containing a dyeing agent in dyeing was one of the major problems in consideration of environmental problems. In other words, it has been desired to develop means for accurately grasping the shape of keratinocytes without a staining step.
【０００３】一方、皮膚より採取した角質細胞の形状の
鑑別法であって、当該角質細胞を紫外線下観察される像
を、画像として取り込むことを特徴とする、角質細胞の
形状の鑑別法は全く知られていないし、この様な手段に
より、染色工程無しに角質細胞の形状を的確に把握でき
ることも全く知られていない。従って、この様な手段を
取ることにより、適時的に迅速な肌の鑑別が、環境にも
優しく行えることも全く知られていない。On the other hand, a method of differentiating the shape of keratinocytes collected from the skin, which is characterized in that an image of the keratinocytes observed under ultraviolet light is captured as an image, there is no method for distinguishing the shape of keratinocytes. It is not known, and it is not known at all that the shape of keratinocytes can be accurately grasped without a staining step by such means. Therefore, it has not been known at all that it is possible to timely and promptly discriminate the skin by taking such a means and being environmentally friendly.
【０００４】[0004]
【発明が解決しようとする課題】本発明は、この様な状
況下為されたものであり、染色工程無しに角質細胞の形
状を的確に把握できる手段を提供することを課題とす
る。The present invention has been made under such circumstances, and it is an object of the present invention to provide means for accurately grasping the shape of keratinocytes without a staining step.
【０００５】[0005]
【課題の解決手段】本発明者らは、この様な状況に鑑み
て、染色工程無しに角質細胞の形状を的確に把握できる
手段を求めて、鋭意研究努力を重ねた結果、皮膚より採
取した角質細胞を紫外線下、顕微鏡乃至はビデオマイク
ロスコープを介して撮影した映像が、角質細胞の形状把
握に好適であることを見出し、発明を完成させるに至っ
た。即ち、本発明は以下に示す技術に関するものであ
る。
（１）皮膚より採取した角質細胞の形状の鑑別法であっ
て、当該角質細胞を紫外線下観察される像を、画像とし
て取り込むことを特徴とする、角質細胞の形状の鑑別
法。
（２）使用する紫外線が、３４０〜３８０ｎｍに極大波
長を持つものであることを特徴とする、（１）に記載の
角質細胞の形状の鑑別法。
（３）画像取り込みが、顕微鏡乃至はビデオマイクロス
コープによるものであることを特徴とする、（１）又は
（２）に記載の角質細胞の形状の鑑別法。
（４）角質細胞が、顔より粘着面を有する媒体によって
採取されたものであることを特徴とする、（１）〜
（３）何れか１項に記載の角質細胞の形状の鑑別法。
（５）粘着面を有する媒体が、粘着組成物を塗工したプ
ラスチック製のディスクであることを特徴とする、
（４）に記載の角質細胞の形状の鑑別法。
（６）皮膚の状態の鑑別を目的とすることを特徴とす
る、（１）〜（５）何れか１項に記載の角質細胞の形状
の鑑別法。
（７）皮膚より採取した角質細胞の形状の鑑別の為の映
像であって、当該角質細胞を紫外線下、顕微鏡乃至はビ
デオマイクロスコープを介して撮影した映像。
（８）使用する紫外線が、３４０〜３８０ｎｍに極大波
長を持つものであることを特徴とする、（７）に記載の
映像。
（９）ＬＥＤ光源を紫外線の光源とすることを特徴とす
る、（７）又は（８）に記載の映像。
（１０）皮膚の状態の鑑別を目的とすることを特徴とす
る、（７）〜（９）何れか１項に記載の映像。
（１１）（７）〜（１０）何れか１項に記載の映像を用
いて、（１）〜（６）何れか１項に記載の角質細胞の鑑
別法により、角質細胞の形状を鑑別し、これを指標とす
ることを特徴とする、皮膚特性の鑑別法。以下に本発明
について更に詳細に説明を加える。[Means for Solving the Problems] In view of such a situation, the present inventors have made earnest research efforts in search of means capable of accurately grasping the shape of keratinocytes without a staining step, and as a result, collected from skin. The inventors have found that an image of keratinocytes photographed under an ultraviolet ray through a microscope or a video microscope is suitable for grasping the shape of keratinocytes, and completed the invention. That is, the present invention relates to the techniques described below. (1) A method for differentiating the shape of keratinocytes collected from the skin, which is characterized in that an image of the keratinocytes observed under ultraviolet light is captured as an image. (2) The method for differentiating the shape of keratinocytes according to (1), wherein the ultraviolet ray used has a maximum wavelength of 340 to 380 nm. (3) The method for differentiating the shape of keratinocytes according to (1) or (2), wherein the image is captured by a microscope or a video microscope. (4) The keratinocytes are collected from the face by a medium having an adhesive surface, (1) to
(3) The method for differentiating the shape of keratinocytes according to any one of items. (5) The medium having an adhesive surface is a plastic disc coated with the adhesive composition,
The method for differentiating the shape of keratinocytes according to (4). (6) The method for differentiating the shape of keratinocytes according to any one of (1) to (5), which is for the purpose of differentiating a skin condition. (7) An image for distinguishing the shape of keratinocytes collected from the skin, which is an image of the keratinocytes photographed under ultraviolet light through a microscope or a video microscope. (8) The image according to (7), wherein the ultraviolet ray used has a maximum wavelength of 340 to 380 nm. (9) The image according to (7) or (8), wherein the LED light source is an ultraviolet light source. (10) The image according to any one of (7) to (9), which is for the purpose of distinguishing a skin condition. (11) Using the image according to any one of (7) to (10), the shape of the keratinocytes is distinguished by the method according to any one of (1) to (6). , A method for distinguishing skin characteristics, which is characterized by using this as an index. The present invention will be described in more detail below.
【０００６】[0006]
【発明の実施の形態】（１）本発明の角質細胞の形状の
鑑別の為の映像
本発明の角質細胞の形状の鑑別の為の映像は、皮膚より
採取した角質細胞の形状の鑑別の為の映像であって、当
該角質細胞を紫外線下、顕微鏡乃至はビデオマイクロス
コープを介して撮影したことを特徴とする。本発明の映
像作成のための角質細胞標本としては、これまで知られ
ている、染色を必須とする角質細胞標本の作り方に準拠
し、染色工程を省いた形で作成することができる。即
ち、顔の頬などの部位より粘着テープや粘着剤を塗工し
たディスクなどの粘着支持体を用いて、ストリッピング
により角質細胞を採取したものが好適に例示できる。粘
着支持体としては、均一性の点でディスクに粘着剤を塗
工した形態が更に好ましく例示できる。この際、更にも
う一度粘着支持体を用いて角質細胞を転写し、角質細胞
の表面側の面を上面にし、この面を観察することもでき
る。この様な転写を行うことは、角質細胞を均一な一層
にすることができるので、角質細胞の形状の理解にとっ
ては好ましい。かくして得られた角質標本に紫外線を照
射し、その結果、紫外線の反射、透過或いは蛍光によっ
て生じる映像が本発明の映像である。即ち、本発明の映
像としては、反射映像でも、透過映像でも或いは蛍光映
像でも構わない。反射映像であれば、標本に対してムラ
の無いように極近傍の周囲から一様に紫外線を照射し、
中心より映像を取得すればよい。透過画像であれば、予
め粘着支持体自身を使用する紫外線透過性のもので調整
し、標本の裏側より紫外線を照射し、透過光が結んだ映
像を取得すればよい。蛍光画像を得るには、上部乃至は
下部より紫外線を照射し、反射乃至は透過の紫外線を必
要に応じてフィルターによって除去し、蛍光によって生
じる可視光のみを集めて映像を取得すればよい。この様
な映像を作るために照射する紫外線としては長波長でも
短波長でも構わないが、特に好ましいものは３４０〜３
８０ｎｍに極大波長を持つものである。この様な紫外線
を提供する光源としてはＬＥＤ光源（ライトエミッティ
ング・ダイオード光源）が好ましく例示できる。この様
な映像取得に特に好ましい機器としては蛍光顕微鏡が挙
げられる。蛍光顕微鏡においては、下部より標本に紫外
線を照射し、透過光乃至は蛍光をレンズで集め、映像を
取得することができる。かかる映像は、デジタルカメラ
などを介してデジタル画像として取り込むことができ
る。又、ビデオマイクロスコープのビデオカメラの部分
を改良して、カメラの周りに紫外線光源を配し、遮光性
のアタッチメントで光源とカメラを覆い、光源からの光
の反射光のみを拾うような形状にして前記の角質細胞標
本の映像を得ることもできる。この様にして得られる本
発明の映像においては角質細胞は明確にその境界が映し
出されるため、染色無しに、本発明の映像によって、そ
の形状を把握することができる。かかる映像は、その境
界も鮮明であるため、コンピューターソフトを利用し
て、面積などを自動的に計測することもできる。BEST MODE FOR CARRYING OUT THE INVENTION (1) Image for distinguishing the shape of keratinocytes of the present invention The image for distinguishing the shape of keratinocytes of the present invention is for distinguishing the shape of keratinocytes collected from skin. The image of the keratinocytes is photographed under ultraviolet light through a microscope or a video microscope. The keratinocyte specimen for producing an image of the present invention can be prepared in accordance with the known method for preparing a keratinocyte specimen that requires staining, omitting the staining step. That is, a suitable example is one in which keratinocytes have been collected by stripping using an adhesive support such as an adhesive tape or a disc coated with an adhesive from a site such as the cheek of the face. As the pressure sensitive adhesive support, a form in which a pressure sensitive adhesive is applied to a disk can be more preferably exemplified in terms of uniformity. At this time, it is also possible to transfer the keratinocytes again using the adhesive support, and make the surface side of the keratinocytes the upper surface and observe this surface. Such transcription is preferable for understanding the shape of keratinocytes because it allows the keratinocytes to form a uniform layer. The image of the present invention is an image generated by irradiating the thus obtained keratinous sample with ultraviolet rays and, as a result, reflection, transmission or fluorescence of the ultraviolet rays. That is, the image of the present invention may be a reflection image, a transmission image, or a fluorescence image. If it is a reflection image, ultraviolet rays are uniformly radiated from the immediate vicinity so that there is no unevenness on the sample,
You only need to get the image from the center. In the case of a transmission image, it may be adjusted in advance with an ultraviolet-transmissive one using the adhesive support itself, and ultraviolet rays are irradiated from the back side of the sample to obtain an image in which transmitted light is connected. In order to obtain a fluorescent image, it is sufficient to irradiate ultraviolet rays from above or below, remove reflected or transmitted ultraviolet rays by a filter as necessary, and collect only visible light generated by fluorescence to obtain an image. Ultraviolet rays for irradiating such an image may have a long wavelength or a short wavelength, but a particularly preferable one is 340 to 3
It has a maximum wavelength of 80 nm. An LED light source (light emitting diode light source) can be preferably exemplified as a light source that provides such ultraviolet rays. A fluorescence microscope is mentioned as a particularly preferable device for such image acquisition. In a fluorescence microscope, an image can be acquired by irradiating a sample with ultraviolet rays from the lower part and collecting transmitted light or fluorescence with a lens. Such an image can be captured as a digital image via a digital camera or the like. In addition, we improved the video camera part of the video microscope, placed an ultraviolet light source around the camera, covered the light source and the camera with a light shielding attachment, and made it a shape that picks up only the reflected light from the light source. It is also possible to obtain an image of the above-mentioned keratinocyte preparation. In the image of the present invention thus obtained, the boundaries of the keratinocytes are clearly displayed, so that the shape of the keratinocytes can be grasped by the image of the present invention without staining. Since the boundary of such an image is clear, computer software can be used to automatically measure the area and the like.
【０００７】（２）本発明の角質細胞の鑑別法、皮膚特
性の鑑別法
本発明の角質細胞の鑑別法は、前記本発明の角質細胞の
形状の鑑別の為の映像を用いることを特徴とする。角質
細胞の形状により、皮膚特性、例えば、肌の刺激に対す
る感受性、バリア機能を含む肌の状態、肌の老化の程度
を表す肌年齢などを知ることができる。この様な情報を
得るための角質細胞の形状としては、角質細胞の配列の
規則性、大きさ（体積或いはその便宜的代替値としての
面積）、一層で剥離してくるか、数層が重なったまま剥
離してくるかという、角質細胞の剥離のパターン等が例
示できる。本発明の映像を用いることにより、これらの
何れもが、角質細胞標本を染色して得た画像と同程度の
精度で鑑別、測定することができる。配列規則性であれ
ば、個々の細胞の形がどの程度六角形になっているかを
肉眼で判定したりすることにより鑑別できるし、大きさ
であれば、二値化などの処理を施した後に、画像処理ソ
フトを使用することにより自動計測することができる。
剥離パーターンは、配列規則性同様に、肉眼判定できる
し、或いは、画像の濃さ（或いは明るさ）などを、輝度
から算出し、推定することもできる。この様な方法につ
いては、染色標本において使用されている技術をそのま
ま応用することができる。又、この様に鑑別された角質
細胞の形状をもとに、従来知られている角質細胞と皮膚
特性の関係を利用して皮膚特性を鑑別することもでき
る。かかる皮膚特性の鑑別が、本発明の皮膚特性の鑑別
法である。この様な技術で、既に知られているものとし
ては、特開２００２−１７６８８号、特開２００１−１
０８６７４号、特開２００１−１３１３８号、特開２０
００−１１６６２３号、特開平１１−３０４７９８号、
特開平１１−２９９７９２号、特開平７−２０９２９２
号等が例示でき、これらの何れもが使用することができ
る。従来の染色標本法が標本調整に、染色剤などを用い
ることができる環境において数時間要していたのを比
べ、本発明の鑑別法は、通常の環境で、ほぼリアルタイ
ムに鑑別を行うことができる。即ち、染色剤などを用い
ることができる環境への角質細胞の移送時間を加味すれ
ば、従来法に比して、本発明の鑑別法では、数日間の時
間短縮が行える。加えて、染色剤を必要としないことか
ら廃水処理の必要性もなく、環境保全の観点からも本発
明の鑑別法は好ましい。この様に、皮膚特性を鑑別する
ことにより、適切な化粧料の選択が可能となり、誤った
化粧料の選択による、トラブルの発生や無駄な投資を避
けることも可能である。又、本発明の鑑別法は、採取し
た角質細胞には非侵襲的であるので、角質細胞の形状の
鑑別を行った後に、種々の染色標本に供することも可能
であり、従来の方法に比して、更に多面的な情報の入手
も可能となる。(2) Method of Differentiating Keratinocytes and Method of Differentiating Skin Properties of the Present Invention The method of differentiating keratinocytes of the present invention is characterized by using an image for distinguishing the shape of keratinocytes of the present invention. To do. From the shape of keratinocytes, it is possible to know skin characteristics such as sensitivity to skin stimuli, skin condition including barrier function, and skin age indicating the degree of skin aging. The shape of the keratinocytes for obtaining such information is the regularity of the arrangement of the keratinocytes, the size (volume or area as a convenient alternative value thereof), or the layers may be exfoliated or several layers may overlap. A pattern of keratinocyte delamination, such as whether the keratin cells are detached as they are, can be exemplified. By using the image of the present invention, any of these can be distinguished and measured with the same accuracy as an image obtained by staining a corneocyte preparation. If it is array regularity, it can be distinguished by visually observing how hexagonal the shape of each cell is, and if it is large, it can be differentiated after undergoing a process such as binarization. Automatic measurement is possible by using image processing software.
Similar to the arrangement regularity, the peeling pattern can be judged by the naked eye, or the density (or brightness) of the image can be calculated and estimated from the luminance. For such a method, the technique used for the stained specimen can be applied as it is. Further, based on the shape of the keratinocytes thus differentiated, the skin characteristics can be distinguished by utilizing the conventionally known relationship between keratinocytes and skin characteristics. Such discrimination of skin characteristics is the method for discriminating skin characteristics of the present invention. Known examples of such techniques are disclosed in JP-A-2002-17688 and JP-A-2001-1.
08674, JP 2001-13138 A, JP 20
00-116623, JP-A-11-304798,
JP-A-11-299792, JP-A-7-209292
And the like, and any of these can be used. In contrast to the conventional stained specimen method that required several hours for preparing a specimen in an environment in which a staining agent or the like can be used, the discrimination method of the present invention can discriminate in real time in a normal environment. it can. That is, in consideration of the transfer time of keratinocytes to the environment where a stain or the like can be used, the differentiation method of the present invention can shorten the time for several days as compared with the conventional method. In addition, since the dyeing agent is not required, there is no need for wastewater treatment, and the identification method of the present invention is preferable from the viewpoint of environmental protection. In this way, by distinguishing the skin characteristics, it is possible to select an appropriate cosmetic material, and it is possible to avoid troubles and unnecessary investment due to incorrect cosmetic material selection. Further, since the differentiation method of the present invention is non-invasive to the collected keratinocytes, it is also possible to subject it to various stained specimens after the shape of the keratinocytes is differentiated, which is in comparison with the conventional method. Then, it becomes possible to obtain more multifaceted information.
【０００８】[0008]
【実施例】以下に、実施例を挙げて、本発明について更
に詳細に説明を加えるが、本発明がかかる実施例にのみ
限定を受けないことは言うまでもない。EXAMPLES The present invention will be described in more detail below with reference to examples, but it goes without saying that the present invention is not limited to these examples.
【０００９】＜実施例１＞粘着テープを頬部に貼付し、
上から均一に抑えた後、剥離し、これを可視光の透過条
件（通常の光学顕微鏡）及び紫外線の透過条件（中心波
長３６０ｎｍ；蛍光顕微鏡）で観察を行った。これを画
像として取り込んだものを図１（図面代用写真）に示
す。可視光透過条件においては、角質細胞の境界は極め
て不明瞭であり、その形状を認知することはできない
が、紫外線透過条件においては、明瞭に角質細胞の形状
が認知でき、面積などが測定できることがわかる。<Example 1> Adhesive tape was attached to the cheek,
After being uniformly suppressed from above, the film was peeled and observed under visible light transmission conditions (normal optical microscope) and ultraviolet light transmission conditions (center wavelength 360 nm; fluorescence microscope). Figure 1 (picture as a substitute for a drawing) shows this captured as an image. Under visible light transmission conditions, the boundaries of keratinocytes are extremely indistinct and their shape cannot be recognized, but under UV transmission conditions, the shape of keratinocytes can be clearly recognized, and area etc. can be measured. Recognize.
【００１０】＜実施例２＞実施例１の紫外線透過条件で
の映像より角質細胞の平均面積を求めた。又、この角質
細胞標本をゲンチアナバイオレットで染色し、従来法に
従って同様に角質細胞の平均面積を求めた。結果を表１
にしめす。これより、本発明の映像より、従来法と同程
度の精度で面積が測定できることがわかる。<Example 2> The average area of keratinocytes was determined from the image of Example 1 under the ultraviolet transmission conditions. Further, this keratinocyte preparation was stained with gentian violet, and the average area of keratinocytes was similarly determined according to the conventional method. The results are shown in Table 1.
Name it. From this, it is understood from the image of the present invention that the area can be measured with the same accuracy as in the conventional method.
【００１１】[0011]
【表１】 [Table 1]
【００１２】[0012]
【発明の効果】本発明によれば、染色工程無しに角質細
胞の形状を的確に把握できる手段を提供することができ
る。According to the present invention, it is possible to provide means for accurately grasping the shape of keratinocytes without a staining step.
【図１】 実施例１の可視光透過映像と本発明の紫外線
透過映像の比較を示す図である。FIG. 1 is a diagram showing a comparison between a visible light transmission image of Example 1 and an ultraviolet transmission image of the present invention.
フロントページの続き (72)発明者 平井 義和 神奈川県横浜市神奈川区高島台27番地１ ポーラ化成工業株式会社横浜研究所内 (72)発明者 平山 賢哉 神奈川県横浜市神奈川区高島台27番地１ ポーラ化成工業株式会社横浜研究所内 Ｆターム(参考） 2G045 CB09 FA16 FA27 FA29 HA06 JA04 5B057 AA10 BA02 CE12 DA01 DB02 DB09 DC04 DC09 DC22 Continued front page (72) Inventor Yoshikazu Hirai 27-1 Takashimadai, Kanagawa-ku, Yokohama-shi, Kanagawa Pola Kasei Co., Ltd. Yokohama Research Center (72) Inventor Kenya Hirayama 27-1 Takashimadai, Kanagawa-ku, Yokohama-shi, Kanagawa Pola Kasei Co., Ltd. Yokohama Research Center F-term (reference) 2G045 CB09 FA16 FA27 FA29 HA06 JA04 5B057 AA10 BA02 CE12 DA01 DB02 DB09 DC04 DC09 DC22
Claims (11)
法であって、当該角質細胞を紫外線下観察される像を、
画像として取り込むことを特徴とする、角質細胞の形状
の鑑別法。1. A method for differentiating the shape of keratinocytes collected from the skin, comprising:
A method for differentiating the shape of keratinocytes, characterized by being captured as an image.
に極大波長を持つものであることを特徴とする、請求項
１に記載の角質細胞の形状の鑑別法。2. The ultraviolet ray used is 340 to 380 nm
The method for differentiating the shape of keratinocytes according to claim 1, wherein the method has a maximum wavelength in.
イクロスコープによるものであることを特徴とする、請
求項１又は２に記載の角質細胞の形状の鑑別法。3. The method for differentiating the shape of keratinocytes according to claim 1 or 2, wherein the image is captured by a microscope or a video microscope.
によって採取されたものであることを特徴とする、請求
項１〜３何れか１項に記載の角質細胞の形状の鑑別法。4. The method for differentiating the shape of keratinocytes according to any one of claims 1 to 3, wherein the keratinocytes are collected from a face by a medium having an adhesive surface.
工したプラスチック製のディスクであることを特徴とす
る、請求項４に記載の角質細胞の形状の鑑別法。5. The method of differentiating the shape of keratinocytes according to claim 4, wherein the medium having an adhesive surface is a plastic disk coated with the adhesive composition.
徴とする、請求項１〜５何れか１項に記載の角質細胞の
形状の鑑別法。6. The method for differentiating the shape of keratinocytes according to any one of claims 1 to 5, which is intended to distinguish the skin condition.
の為の映像であって、当該角質細胞を紫外線下、顕微鏡
乃至はビデオマイクロスコープを介して撮影した映像。7. An image for differentiating the shape of keratinocytes collected from the skin, the keratinocytes being photographed under ultraviolet light through a microscope or a video microscope.
に極大波長を持つものであることを特徴とする、請求項
７に記載の映像。8. The ultraviolet ray used is 340 to 380 nm
The image according to claim 7, characterized in that it has a maximum wavelength in.
特徴とする、請求項７又は８に記載の映像。9. The image according to claim 7, wherein the LED light source is an ultraviolet light source.
特徴とする、請求項７〜９何れか１項に記載の映像。10. The image according to any one of claims 7 to 9, wherein the image is for distinguishing a skin condition.
像を用いて、請求項１〜６何れか１項に記載の角質細胞
の鑑別法により、角質細胞の形状を鑑別し、これを指標
とすることを特徴とする、皮膚特性の鑑別法。11. The shape of keratinocytes is discriminated by the method for discriminating keratinocytes according to any one of claims 1 to 6, using the image according to any one of claims 7 to 10. A method for distinguishing skin characteristics, characterized by using as an index.
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