JP2003240775A - Housing container for tissue piece - Google Patents
Housing container for tissue pieceInfo
- Publication number
- JP2003240775A JP2003240775A JP2002042693A JP2002042693A JP2003240775A JP 2003240775 A JP2003240775 A JP 2003240775A JP 2002042693 A JP2002042693 A JP 2002042693A JP 2002042693 A JP2002042693 A JP 2002042693A JP 2003240775 A JP2003240775 A JP 2003240775A
- Authority
- JP
- Japan
- Prior art keywords
- container
- tissue piece
- tissue
- liquid surface
- cold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Details Of Rigid Or Semi-Rigid Containers (AREA)
- Packages (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、生物から採取され
た組織片を無菌状態で収容するための容器に関する。詳
しくは、前記組織片の保管及び移送作業を迅速かつ容易
に行うことができ、更には、移送過程において収容され
た組織片が損傷しないように工夫された組織片収容容器
に関する。TECHNICAL FIELD The present invention relates to a container for aseptically containing a piece of tissue collected from an organism. More specifically, the present invention relates to a tissue piece storage container that can store and transfer the tissue piece quickly and easily and is devised so that the tissue piece stored in the transfer process is not damaged.
【０００２】[0002]
【従来の技術】近年、動植物から採取した組織片の培養
を行う組織培養技術が発展し、種々の分野で活用されて
いる。例えば、ヒト細胞から採取された組織片を増殖さ
せることによって得られた組織体を、皮膚や臓器等の損
傷又は欠損部位に定着させて、該部位の組織再生を図
る、いわゆる「再生医療分野」においては、前記組織培
養技術は基本かつ重要な技術である。2. Description of the Related Art In recent years, a tissue culture technique for culturing tissue pieces collected from animals and plants has been developed and utilized in various fields. For example, so-called "regenerative medicine field" in which a tissue body obtained by proliferating a piece of tissue collected from human cells is settled in a damaged or defective site of skin, organ, etc., and tissue regeneration of the site is attempted. In the above, the tissue culture technique is a basic and important technique.
【０００３】かかる組織培養技術では、採取された組織
が細菌やウイルス等に感染することがないように、採取
した組織を確実かつ効率よく、完全無菌状態で分離・増
殖できることが重要である。また、組織の分離・増殖過
程では、病原菌の浸入を防ぐため、組織に空隙、剥離そ
の他の損傷を発生させないようにすることも重要であ
る。更に、採取された組織片が体表組織や粘膜等である
場合には、該組織片は汚染されている可能性があるの
で、採取後直ちに抗生物質を含む培養液（培地）に浸漬
し、消毒後に、細胞の分離・増殖を行う必要がある。In such a tissue culture technique, it is important that the collected tissue can be separated and grown in a completely aseptic state reliably and efficiently so that the collected tissue is not infected with bacteria or viruses. Further, in the process of separating / proliferating the tissue, it is important to prevent voids, peeling and other damages in the tissue in order to prevent invasion of pathogenic bacteria. Furthermore, when the collected tissue piece is body surface tissue or mucous membrane, since the tissue piece may be contaminated, it is immersed in a culture solution (medium) containing an antibiotic immediately after collection, After disinfection, it is necessary to separate and grow cells.
【０００４】[0004]
【発明が解決しようとする課題】しかしながら、従来の
組織培養技術においては、採取された組織片を、一時保
管して所定の処理施設に移送する際には、無菌のリン酸
バッファー溶液その他の塩類溶液に該組織片を浸漬させ
て、密封された容器内に収容しておく作業が必須になる
にもかかわらず、無菌の塩類溶液を都度用意する作業又
は手配には手間がかかり、組織片の分離・増殖作業を迅
速かつ簡易に行うことができなかった。However, in the conventional tissue culture technique, when the collected tissue pieces are temporarily stored and transferred to a predetermined processing facility, a sterile phosphate buffer solution and other salts are used. Although it is essential to immerse the tissue piece in a solution and store it in a sealed container, it is time-consuming to prepare or prepare a sterile saline solution each time, and The separation / proliferation work could not be performed quickly and easily.
【０００５】また、組織片を収容する容器自体について
も、既存のシャーレやフラスコ等の容器を流用してお
り、また、運搬容器も好適なものが存在しなかったの
で、移送・運搬過程の振動等によって、組織片が損傷す
るおそれがあった。Further, as the container itself for storing the tissue piece, the existing container such as a petri dish or a flask is diverted, and there is no suitable transport container. There is a possibility that the tissue piece may be damaged by the above.
【０００６】そこで、本発明は、採取された組織片を保
管及び移送するために好適な専用の組織片収容容器並び
に保冷運搬容器を提供することを主な目的とする。[0006] Therefore, the main object of the present invention is to provide a dedicated tissue piece accommodating container and a cold transport container suitable for storing and transferring collected tissue pieces.
【０００７】[0007]
【課題を解決するための手段】上記技術的課題を解決す
るために、本願では次の手段を提供する。In order to solve the above technical problems, the present application provides the following means.
【０００８】まず、生物から採取された組織片を無菌状
態で収容するための容器であって、前記容器を閉塞する
蓋に、該容器に所定量収容される溶液の液面を封止する
液面封止部位が設けられた組織片収容容器を提供する。
該容器は、例えば、透明なポリカーボネートその他の合
成樹脂によって成形でき、液面封止部位は、蓋から容器
開口部に向けて突設する支持棒の先端部位に固定された
薄板状のフィルター等によって形成することができる。
なお、本組織片収容容器に収容する組織片は、動物、植
物由来の組織片を広く包含し、狭く解釈されない。First, a container for aseptically containing a tissue piece collected from a living organism, a lid for closing the container, and a liquid for sealing a liquid surface of a solution contained in a predetermined amount in the container. Provided is a tissue piece accommodating container provided with a surface sealing part.
The container can be molded of, for example, transparent polycarbonate or other synthetic resin, and the liquid surface sealing portion is formed by a thin plate filter or the like fixed to the tip portion of the support rod protruding from the lid toward the container opening. Can be formed.
It should be noted that the tissue pieces to be stored in the present tissue piece storage container broadly include tissue pieces derived from animals and plants and are not narrowly interpreted.
【０００９】この構成の組織片収容容器によれば、組織
片が浸漬されている溶液の液面が、前記液面封止部位に
よって封止されているので、移送又は運搬過程での振動
によって、容器内の溶液が揺れ動いたり、泡立ったり、
撹拌されたりすることがない。このため、移送又は運搬
過程において、組織片が溶液内を浮遊したり、動いたり
してしまうことがなくなって、溶液内に組織片が静置さ
れるので、組織片に損傷が発生することがない。According to the tissue piece accommodating container of this structure, since the liquid surface of the solution in which the tissue piece is immersed is sealed by the liquid surface sealing portion, it is possible to prevent vibration due to vibration during the transfer or transportation process. The solution in the container shakes, foams,
It is not agitated. Therefore, during the transfer or transportation process, the tissue piece does not float or move in the solution, and the tissue piece is allowed to stand in the solution, which may cause damage to the tissue piece. Absent.
【００１０】ここで、前記組織片収容容器では、容器に
対する蓋の嵌合度合を調節することによって、前記液面
封止部位が前記液面に接着する状態と接着しない状態を
適宜選択できるように工夫する。例えば、容器に対する
蓋のねじ込み作業を二段階で行うことができるように
し、まず、第１段階では、前記液面封止部位が容器に所
定量収容された溶液の液面に接着しない状態を保持し、
更にねじ込む第２段階では、前記液面に接着する状態と
なるように工夫することができる。Here, in the tissue piece accommodating container, by adjusting the fitting degree of the lid with respect to the container, it is possible to appropriately select a state in which the liquid surface sealing portion is adhered to the liquid surface and a state in which the liquid surface sealing portion is not adhered. Devise. For example, the operation of screwing the lid onto the container can be performed in two steps. First, in the first step, the state where the liquid surface sealing portion is not adhered to the liquid surface of the solution stored in the container in a predetermined amount is maintained. Then
Further, in the second step of screwing, it can be devised so as to be in a state of being bonded to the liquid surface.
【００１１】この構成によって、溶液に組織片が浸漬さ
れた場合だけを選択して、前記液面封止部位によって液
面を封止することができる。With this configuration, the liquid surface can be sealed by the liquid surface sealing portion only when the tissue piece is immersed in the solution.
【００１２】また、上記組織片収容容器では、容器の底
部を略円錐状に形成する。この構成によって、容器に収
容された組織片はその自重によって、尖った最深部位に
沈んできて静置することになるので、組織片の存在及び
状態が容易に視認できるようになり、また組織片も静置
状態を保持し易くなる。Further, in the above-mentioned tissue piece accommodating container, the bottom of the container is formed into a substantially conical shape. With this structure, the tissue piece contained in the container is sunk by its own weight to the sharpest deepest portion and left to stand, so that the existence and state of the tissue piece can be easily visually recognized. Also, it becomes easy to maintain the stationary state.
【００１３】ここで、前記したような略円錐状の底部で
は、容器を立て置きすることができないので、組織片収
容作業等や時に不便となることが想定される。そこで、
この尖った容器底部に対して、容器を立て置きするため
の専用の底部材を装着できるように工夫する。なお、底
部材は、例えば、上方に開口し、底面が平坦な円筒状の
透明部材を前記容器底部に螺着又は嵌着する形態が考え
られる。Here, since it is not possible to stand the container upright on the substantially conical bottom portion as described above, it is assumed that it is inconvenient for the work of accommodating the tissue piece or the like. Therefore,
A devised bottom member for standing the container upright can be attached to the pointed container bottom. The bottom member may be, for example, a cylindrical transparent member having an open upper surface and a flat bottom surface, which is screwed or fitted to the bottom of the container.
【００１４】次に、本発明では、生物から採取された組
織片を無菌状態で収容するための容器であって、塩類と
抗生物質を少なくとも含有する無菌溶液が、予め所定
量、密封収容された組織片収容容器を提供する。塩類
は、例えばリン酸、抗生物質は例えばペニシリン、スト
レプトマイシン、バンコマイシン等を挙げることがで
き、目的に応じて適宜選択、組み合わせることができ
る。無菌溶液はガーゼなどに浸漬させてタッパー状又は
シャーレ状の容器に収容しておいてもよいし、容器に注
入しておいてもよい。前記無菌溶液には、塩類、抗生物
質に加えて、グリセリンや粘度調整のための糖（特に植
物由来の糖）等を配合してもよい。Next, according to the present invention, a container for aseptically containing a tissue piece collected from an organism, in which a predetermined amount of a sterile solution containing at least salts and antibiotics is hermetically sealed in advance. A tissue piece storage container is provided. Examples of salts include phosphoric acid, and examples of antibiotics include penicillin, streptomycin, vancomycin, etc., which can be appropriately selected and combined according to the purpose. The sterile solution may be dipped in gauze or the like to be stored in a tapper-shaped or petri dish-shaped container, or may be poured into the container. In addition to salts and antibiotics, glycerin, sugar for viscosity adjustment (particularly sugar of plant origin), and the like may be added to the sterile solution.
【００１５】この構成の組織片収容容器は、組織片を無
菌状態で保管できる抗生物質入りバッファー溶液（塩類
溶液）が、好適な配合、濃度及び容量で予め収容された
状態で作業者（利用者）に提供されることになるので、
作業者は前記溶液の調整又は手配、準備作業を一切行わ
なくてもよい。また、該溶液の容器への注入作業も必要
がない。従って、採取した組織片を容器内に収容し、密
封保管するという作業を迅速に行うことができるように
なる。なお、この容器においても、蓋に上記液面封止部
位を設けるようにしてもよい。The tissue piece accommodating container of this structure is a worker (user) in a state where an antibiotic-containing buffer solution (salt solution) capable of storing tissue pieces in an aseptic state is contained in advance in a suitable formulation, concentration and volume. ) Will be provided to
The operator does not have to perform any adjustment, arrangement, or preparation work of the solution. Further, there is no need to inject the solution into the container. Therefore, the work of accommodating the collected tissue piece in the container and hermetically storing it can be quickly performed. Incidentally, also in this container, the lid may be provided with the liquid surface sealing portion.
【００１６】上記説明した組織片収容容器は、組織片を
確実に無菌状態で保管・移送することができ、組織片の
損傷が発生しないことから、再生医療に用いられるの
が、特に好適である。The above-described container for accommodating a tissue piece is particularly suitable for regenerative medicine because it can reliably store and transfer the tissue piece in an aseptic state without causing damage to the tissue piece. .
【００１７】次に、本願では、上記組織片収容容器が嵌
着できるように形成された穴を複数備える容器配置板
と、該容器配置板が、容器開口部に取り付けられ、保冷
機能を有する外容器と、該外容器の内側に設けられた保
冷剤収容部と、を備え、前記穴に嵌着された前記組織片
容器の組織片収容領域が、前記保冷剤からの冷気に曝さ
れるように構成された保冷運搬容器を提供する。この保
冷運搬容器を用いれば、組織片収容容器を迅速に配置
し、冷温下で運搬することが可能となる。Next, in the present application, a container arrangement plate having a plurality of holes formed so that the tissue piece accommodating container can be fitted therein, and the container arrangement plate is attached to the container opening and has an outside function having a cold insulation function. The tissue piece storage region of the tissue piece container, which is equipped with a container and a cooling agent storage portion provided inside the outer container, and which is fitted into the hole, is exposed to cold air from the cooling agent. There is provided a cold carrier container configured as described above. By using this cold-keeping transport container, the tissue piece storage container can be quickly arranged and transported at a cool temperature.
【００１８】以上のように、本発明は、採取された組織
片を簡単に保管、移送・運搬できる容器を提供するとい
う技術的意義、特に再生医療の現場で有用な容器を提供
するという技術的意義を有している。As described above, the present invention has the technical significance of providing a container capable of easily storing, transferring, and transporting collected tissue pieces, and in particular, provides a container useful in the field of regenerative medicine. It has significance.
【００１９】[0019]
【発明の実施の形態】次に、本発明に係る組織片収容容
器並びに保冷運搬容器の好適な実施形態について、添付
した図面を参照しながら説明する。BEST MODE FOR CARRYING OUT THE INVENTION Next, preferred embodiments of a tissue piece storage container and a cold transport container according to the present invention will be described with reference to the accompanying drawings.
【００２０】まず、図１は、本発明に係る組織片収容容
器の好適な実施形態の部品構成を示す図である。この図
１に示された組織片収容容器は、上方に開口する略円筒
状の容器１と、該容器１を閉塞する蓋２と、を備えてい
る。容器１並びに蓋２は、例えば、ポリカーボネートや
ポリエチレン等の透明合成樹脂で形成することができ
る。First, FIG. 1 is a view showing a component structure of a preferred embodiment of a tissue piece accommodating container according to the present invention. The tissue piece accommodating container shown in FIG. 1 includes a substantially cylindrical container 1 that opens upward, and a lid 2 that closes the container 1. The container 1 and the lid 2 can be formed of a transparent synthetic resin such as polycarbonate or polyethylene, for example.
【００２１】前記容器１は、円筒状の胴体部１１と、該
胴体部１１の下方に形成された略円錐状の底部１２と、
からなり、容器１の内部には、少なくとも塩類と抗生物
質が含まれた無菌溶液４が収容される。胴体部１１の上
端部には、蓋２が螺着されるねじ山１４が切られてお
り、更に胴体部１１の下端部には、底部材３が螺着され
るねじ山１５が切られている。なお、符号１３は、容器
１の開口部を表しており、この開口部１３から採取され
た組織片５が、前記無菌溶液４に投入される。The container 1 has a cylindrical body portion 11 and a substantially conical bottom portion 12 formed below the body portion 11.
The container 1 contains a sterile solution 4 containing at least salts and antibiotics. A screw thread 14 with which the lid 2 is screwed is cut at the upper end of the body 11, and a screw thread 15 with which the bottom member 3 is screwed is cut at the lower end of the body 11. There is. Reference numeral 13 represents an opening of the container 1, and the tissue piece 5 collected from the opening 13 is put into the sterile solution 4.
【００２２】底部材３は、容器１をテーブル上に立て置
きして用いる場合等に、容器１の底部に取り付けられる
付属部材である。この底部材３は、平坦な底面３１（上
げ底でもよい。）を備え、上方開口する内部空洞の円筒
状の容器状を呈している。なお、この底部材３も、容器
１と同じ合成樹脂で形成する。図１中の符号３２は、底
部材３の上方開口部、符号３３は、容器１の前記ねじ山
１５に螺合するねじ部を表している。The bottom member 3 is an accessory member attached to the bottom of the container 1 when the container 1 is used by standing it on a table. The bottom member 3 has a flat bottom surface 31 (may be a raised bottom), and has a cylindrical container shape having an internal cavity that opens upward. The bottom member 3 is also made of the same synthetic resin as the container 1. Reference numeral 32 in FIG. 1 denotes an upper opening portion of the bottom member 3, and reference numeral 33 denotes a screw portion screwed into the screw thread 15 of the container 1.
【００２３】ここで、蓋２は、符号２２で示された「液
面封止部位」が設けられている。この液面封止部位２２
は、蓋２の天面２１（の図示しない下面）に固定されて
垂下する支持軸２２１と、該支持軸２２１の下端部に固
定された円盤状の封止板２２２と、から構成されてい
る。封止板２２２は、無菌溶液４の液面に接着して、移
送・運搬過程での無菌溶液４の揺動を防ぐという機能を
発揮する。Here, the lid 2 is provided with a "liquid surface sealing portion" indicated by reference numeral 22. This liquid level sealing portion 22
Is composed of a support shaft 221 fixed to (the lower surface (not shown) of) the lid 2 and hanging down, and a disc-shaped sealing plate 222 fixed to the lower end of the support shaft 221. . The sealing plate 222 adheres to the liquid surface of the sterile solution 4 and exhibits the function of preventing the swinging of the sterile solution 4 during the transfer / transport process.
【００２４】この封止板２２２は、容器１の内口径より
もやや小さい口径に形成された円形枠体２２２ａと、前
記支持軸２２１の下端に結合し、前記枠体２２２ｂに向
けて放射状に延びる複数の軸枠２２２ｂと、円形枠体２
２２ａ及び軸枠２２２ｂに適宜の手段で固定された膜体
２２２ｃと、から構成されている。なお、液面封止部位
２２の形態は、前記構成に限定するものではなく、液面
を封止できる形態であれば適宜採用できる。The sealing plate 222 is connected to a circular frame 222a having a diameter slightly smaller than the inner diameter of the container 1 and the lower end of the support shaft 221, and extends radially toward the frame 222b. The plurality of shaft frames 222b and the circular frame body 2
22a and a film body 222c fixed to the shaft frame 222b by an appropriate means. The form of the liquid surface sealing portion 22 is not limited to the above-described configuration, and any form that can seal the liquid surface can be appropriately adopted.
【００２５】次に、図２は、同組織片収容容器の組織片
保管時の状態を示す外観斜視図である。この図２に示す
ように、組織片５が収容された容器１は、蓋２で密封さ
れ、無菌溶液４の液面は前記封止板２２２によって封止
されている。なお、組織片５は、その自重によって、底
部１２の最深部１２ａ付近に沈んで静置されることにな
るので、その存在又は状態が視認し易い。Next, FIG. 2 is an external perspective view showing the state of the tissue piece storage container during storage of the tissue piece. As shown in FIG. 2, the container 1 accommodating the tissue piece 5 is sealed with a lid 2, and the liquid surface of the sterile solution 4 is sealed with the sealing plate 222. Since the tissue piece 5 is sunk and settled near the deepest portion 12a of the bottom 12 due to its own weight, its existence or state is easily visible.
【００２６】図２に示された状態で移送した場合には、
組織片５が浸漬されている無菌溶液４の液面が、前記液
面封止部位２２によって封止されているので、移送過程
での振動で該溶液４が揺れ動いたり、泡立ったり、撹拌
されたりすることがない。このため、移送過程におい
て、組織片５が溶液４内を浮遊したり、動いたりしてし
まうことがなくなる。即ち、移送・運搬中においても無
菌溶液４内に組織片５が静置され続けるので、該組織片
５に損傷が発生することがない。When transferred in the state shown in FIG. 2,
Since the liquid surface of the sterile solution 4 in which the tissue piece 5 is immersed is sealed by the liquid surface sealing portion 22, the solution 4 is shaken, foamed, or agitated by the vibration in the transfer process. There is nothing to do. Therefore, the tissue piece 5 will not float or move in the solution 4 during the transfer process. That is, since the tissue piece 5 continues to stand in the sterile solution 4 even during the transportation / transportation, the tissue piece 5 is not damaged.
【００２７】次に、図３に基づいて本発明に係る組織片
収容容器の変形例の構成について説明する。なお、図３
（Ａ）は、液面封止部位の封止板２２が液面４１に接着
してない状態を示す図、図３（Ｂ）は、液面封止部位の
封止板２２が液面４１に接着した状態を示す図である。Next, the structure of a modified example of the tissue piece accommodating container according to the present invention will be described with reference to FIG. Note that FIG.
FIG. 3A is a diagram showing a state in which the sealing plate 22 at the liquid surface sealing portion is not adhered to the liquid surface 41, and FIG. 3B is a diagram showing the sealing plate 22 at the liquid surface sealing portion being at the liquid surface 41. It is a figure which shows the state adhered to.
【００２８】この変形例に係る組織片収容容器では、容
器１に対する蓋２の嵌合度合を調節することによって、
液面封止部位２２の封止板２２２が液面４１に接着する
状態と接着しない状態とを適宜選択できるように工夫さ
れている。In the tissue piece accommodating container according to this modification, by adjusting the degree of fitting of the lid 2 to the container 1,
It is devised so that the state where the sealing plate 222 of the liquid surface sealing portion 22 is adhered to the liquid surface 41 or the state where it is not adhered can be appropriately selected.
【００２９】具体的には、容器１に対する蓋２のねじ込
み作業を二段階で行うことができるようにする。まず、
図３（Ａ）に示す第１段階では、封止板２２２が容器１
に所定量収容された無菌溶液４の液面４１に接着しない
状態が保持され、更に蓋２が下方にねじ込まれた図３
（Ｂ）に示す第２段階では、封止板２２２が前記液面４
１に接着する状態となる。この構成によって、溶液４に
組織片５が浸漬され、移送・運搬する場合だけを選択し
て、液面４１を封止することができるようになるので便
利である。Specifically, the operation of screwing the lid 2 onto the container 1 can be performed in two steps. First,
In the first stage shown in FIG. 3A, the sealing plate 222 is the container 1
The state in which the sterile solution 4 contained in a predetermined amount is not adhered to the liquid surface 41 and the lid 2 is further screwed downward is shown in FIG.
At the second stage shown in (B), the sealing plate 222 causes the liquid level 4
It is in a state of being bonded to 1. With this configuration, the tissue piece 5 is immersed in the solution 4, and the liquid surface 41 can be sealed by selecting only when the tissue piece 5 is transferred / transported.
【００３０】以上説明した組織片収容容器は、予め無菌
溶液４を所定量収容した状態で利用者に提供することが
でき、また、図４に示すように、無菌溶液４をガーゼ６
などに浸漬させて、符号７で示すようなシャーレ状容器
７に収容しておいてもよい。なお、図４に示す符号８
は、前記シャーレ状容器７の蓋を表している。The container for accommodating tissue pieces described above can be provided to the user in a state in which a predetermined amount of the sterile solution 4 has been stored in advance, and as shown in FIG.
Alternatively, it may be dipped into a petri dish-shaped container 7 as shown by reference numeral 7 and then stored. Note that reference numeral 8 shown in FIG.
Represents the lid of the dish-shaped container 7.
【００３１】このような組織片収容容器では、組織片５
を無菌状態で保管できる抗生物質入り塩類溶液（バッフ
ァー溶液）４が、好適な配合、濃度及び容量で予め収容
された状態で作業者に提供されることになるので、作業
者は前記溶液の調整又は手配、準備作業を一切行わなく
てもよくなる。また、該溶液４の容器１又は７への注入
作業も必要がない。In such a tissue piece storage container, the tissue piece 5
The salt solution (buffer solution) 4 containing antibiotics, which can be stored aseptically, will be provided to the worker in a pre-stored state with a suitable formulation, concentration and volume, so that the worker can adjust the solution. Alternatively, no arrangement or preparation work is required. Further, there is no need to inject the solution 4 into the container 1 or 7.
【００３２】次に、図５〜図７に基づいて、上記説明し
た組織片収容容器１の好適な保冷運搬容器について説明
する。なお、図５は、同保冷運搬容器の外観斜視図、図
６は、図５のＩ−Ｉ線断面図、図７は、同保冷運搬容器
の上部縦断面図、である。Next, with reference to FIGS. 5 to 7, a suitable cold carrier container for the tissue piece container 1 described above will be described. 5 is an external perspective view of the cold-reservoir carrying container, FIG. 6 is a cross-sectional view taken along the line I-I of FIG. 5, and FIG.
【００３３】図５〜図７において符号９で示された保冷
運搬容器は、上記構成の容器１を備える組織片収容容器
が嵌め込むことができるように形成された穴Ｈが複数形
成された円形の容器配置板９１を備えている。この容器
配置板９１は、アクリル樹脂その他の合成樹脂で形成さ
れている。容器配置板９１、保冷機能を有する材料、例
えば発泡スチロールによって形成された外容器９２の開
口部Ｓ（図７参照）の周壁所定箇所に着座するように取
り付けられる（図５、図７参照）。The cold carrier container indicated by reference numeral 9 in FIGS. 5 to 7 is a circular shape having a plurality of holes H formed therein so that the tissue piece accommodating container including the container 1 having the above-mentioned configuration can be fitted therein. The container placement plate 91 of FIG. The container placement plate 91 is made of acrylic resin or other synthetic resin. The container arranging plate 91 and a material having a cold-keeping function, for example, is attached so as to be seated on a predetermined position on the peripheral wall of the opening S (see FIG. 7) of the outer container 92 formed of expanded polystyrene (see FIGS. 5 and 7).
【００３４】ここで、外容器９２の内周囲には、保冷剤
１０が収容される保冷剤収容部９３が設けられている。
収容された保冷剤１０から発せられた冷気Ｃは、保冷剤
収容部９３を形成する仕切り壁９４に配設された多数の
孔９４１を通過して、内部空洞部９５に流入する。これ
により、容器配置板９１に配置された組織片収容容器
１，１・・・は、前記冷気Ｃに曝され、移送・運搬過程
において４〜５℃の冷温状態に保持される。Here, on the inner periphery of the outer container 92, there is provided a cold preservation agent accommodating portion 93 for accommodating the cold preservation agent 10.
The cold air C emitted from the stored cold insulating agent 10 passes through the multiple holes 941 arranged in the partition wall 94 forming the cold insulating agent containing portion 93 and flows into the internal cavity 95. As a result, the tissue piece accommodating containers 1, 1 ... Arranged on the container arrangement plate 91 are exposed to the cold air C, and are kept in a cold temperature state of 4 to 5 ° C. during the transfer / transport process.
【００３５】図７における符号９６は、保冷容器９２を
閉塞する蓋を表している。該蓋９６は、例えば透明な合
成樹脂等によって形成され、保冷容器９３の上端外周に
形成された凸部９２１に嵌着される。Reference numeral 96 in FIG. 7 represents a lid for closing the cold insulation container 92. The lid 96 is formed of, for example, a transparent synthetic resin or the like, and is fitted to a convex portion 921 formed on the outer periphery of the upper end of the cold insulating container 93.
【００３６】ここで、蓋９６の下面９６１には、容器配
置板９１に配置された組織片収容容器１，１・・・の動
きを抑止するための円盤状の凸部９６２が下方に突設さ
れている。凸部９６２で抑えられた組織片収容容器１，
１・・・は、移送・運搬中においても容器配置板９１か
ら外れてしまうことがなくなる。また、容器配置版９１
自体も、組織片収容容器１，１・・・の抑止作用を介し
て、外容器９２に押し付けられるので、外容器９２から
はずれてしまうことがない。Here, on the lower surface 961 of the lid 96, a disc-shaped convex portion 962 for restraining the movement of the tissue piece accommodating containers 1, 1 ... Arranged on the container arrangement plate 91 is projected downward. Has been done. Tissue piece storage container 1 suppressed by the convex portion 962
1 does not come off from the container placement plate 91 even during transportation / transportation. Also, the container placement plate 91
Since the tissue itself is also pressed against the outer container 92 via the restraining action of the tissue piece storage containers 1, 1, ..., It does not come off from the outer container 92.
【００３７】なお、蓋下面９６１に凸部９６２を突設さ
せた上記構成の他にも、蓋９６自体を厚く形成したり、
蓋９６が保冷容器９２に嵌着したときの下面９６１の位
置が組織片収容容器１，１・・・に当接するようにした
りすることによって、蓋下面９６１で組織片収容容器
１，１・・・を抑える構成を採用してもよい。In addition to the above structure in which the convex portion 962 is provided on the lower surface 961 of the lid, the lid 96 itself may be formed thicker,
The position of the lower surface 961 when the lid 96 is fitted into the cold insulation container 92 is brought into contact with the tissue piece accommodating containers 1, 1, ..., By the lid lower surface 961, the tissue piece accommodating containers 1, 1, ... -A configuration that suppresses may be adopted.
【００３８】[0038]
【発明の効果】本発明によれば、主に次の効果が奏され
る。According to the present invention, the following effects are mainly exhibited.
【００３９】（１）本発明に係る組織片収容容器によれ
ば、組織片が浸漬されている溶液の液面が、前記液面封
止部位によって封止されているので、移送・運搬過程で
の振動によって、容器内の溶液が揺れ動いたり、泡立っ
たり、撹拌されたりすることがないため、組織片が溶液
内を浮遊したり、動いたりしてしまうことがなく、組織
片に損傷が生じるおそれがない。従って、組織片を所望
の場所に安心して移送・運搬することができ、また、移
送・運搬されてきた組織片は、再生医療等で確実に利用
できる状態で提供されることになる。(1) According to the tissue piece accommodating container of the present invention, since the liquid surface of the solution in which the tissue piece is immersed is sealed by the liquid surface sealing portion, it is possible to perform the transfer / transport process. The solution in the container does not shake, foam, or agitate due to the vibration of the tissue, so the tissue piece does not float or move in the solution, which may damage the tissue piece. There is no. Therefore, the tissue piece can be safely transferred / transported to a desired place, and the tissue piece transferred / transported is provided in a state that can be reliably used in regenerative medicine or the like.
【００４０】（２）蓋の嵌合度合を調節することによっ
て、前記液面封止部位が前記液面に接着する状態と接着
しない状態とを適宜選択できるように工夫することによ
って、溶液に組織片が浸漬された場合だけを選択して、
前記液面封止部位によって液面を封止することができ
る。(2) By adjusting the degree of fitting of the lid, it is possible to appropriately select the state in which the liquid surface sealing portion adheres to the liquid surface and the state in which the liquid surface sealing portion does not adhere to the liquid surface. Select only if the piece is dipped,
The liquid surface can be sealed by the liquid surface sealing portion.
【００４１】（３）また、容器の底部を略円錐状に形成
することによって、容器に収容された組織片が自重によ
って、尖った最深部位に沈んできて静置することになる
ので、組織片の存在及び状態が容易に視認できるように
なる。また、遠心分離器にも挿着し易い。(3) Further, by forming the bottom portion of the container into a substantially conical shape, the tissue piece accommodated in the container is sunk into the sharpest deepest portion by its own weight and is left to stand. The presence and state of the can be easily visually recognized. It is also easy to insert into a centrifuge.
【００４２】（４）略円錐状の容器底部に対して、容器
を立て置きするための底部材を装着させれば、テーブル
等に並列させた容器に対して、組織片を収容する作業を
簡易に行うことができる。(4) If a bottom member for standing the container upright is attached to the substantially conical container bottom, the work of accommodating the tissue pieces in the containers arranged in parallel on the table or the like can be simplified. Can be done.
【００４３】（５）塩類と抗生物質を少なくとも含有す
る無菌溶液が、予め所定量、密封収容された組織片収容
容器を提供することによって、作業者は前記溶液の調整
又は手配、準備作業を一切行わなくてもよくなり、該溶
液の容器への検量・注入作業も必要がなくなるので大変
便利である。(5) By providing a tissue piece accommodating container in which a predetermined amount of a sterile solution containing at least salts and antibiotics is hermetically sealed in advance, the operator does not have to perform any adjustment, arrangement or preparation work of the solution. This is very convenient because it is not necessary to perform it, and the work of calibrating and injecting the solution into the container is not necessary.
【００４４】（６）本発明に係る保冷保管容器によれ
ば、組織片収容容器を迅速に配置し、組織片を冷温下で
確実に運搬することができる。(6) According to the cold storage container of the present invention, the tissue piece accommodating container can be quickly arranged and the tissue piece can be reliably transported at a cool temperature.
【００４５】（７）本発明に係る組織片収容容器並びに
保冷運搬容器は、組織片を確実に無菌状態で保管・移送
することができ、組織片に対する損傷の発生を防止でき
るように工夫されていることから、再生医療の現場で用
いられるのが、特に好適である。(7) The tissue piece accommodating container and the cold transport container according to the present invention are devised so that the tissue piece can be reliably stored and transferred in an aseptic state and damage to the tissue piece can be prevented. Therefore, it is particularly suitable to be used in the field of regenerative medicine.
【図１】本発明に係る組織片収容容器の好適な実施形態
の部品構成を示す図FIG. 1 is a diagram showing a component structure of a preferred embodiment of a tissue piece storage container according to the present invention.
【図２】同組織片収容容器の組織片保管時の一態様を示
す外観斜視図FIG. 2 is an external perspective view showing one aspect of the tissue piece storage container during storage of tissue pieces.
【図３】（Ａ）液面封止部位（２２）の封止板（２２
２）が液面（４１）に接着してない状態を示す図
（Ｂ）同部位（２２）の封止板（２２２）が液面（４
１）に接着した状態を示す図FIG. 3 (A) Sealing plate (22) of liquid level sealing portion (22)
FIG. 2B shows a state in which 2) is not adhered to the liquid surface (41). (B) The sealing plate (222) at the same portion (22) shows the liquid surface (4).
Figure showing the state of being bonded to 1)
【図４】予め無菌溶液（４）が収容された組織片収容器
の一実施形態を表す図FIG. 4 is a diagram showing an embodiment of a tissue piece container in which a sterile solution (4) is stored in advance.
【図５】保冷運搬容器（９）の外観斜視図FIG. 5 is an external perspective view of a cold carrier (9).
【図６】図５のＩ−Ｉ線断面図6 is a sectional view taken along line I-I of FIG.
【図７】同保冷運搬容器（９）の上部縦断面図、FIG. 7 is a vertical cross-sectional view of the upper part of the cold-storage transport container (9).
１ 容器 ２ 蓋 ３ 底部材 ４ 無菌溶液 ５ 組織片 ９ 保冷運搬容器 １０ 保冷剤 １２ （容器１の）底部 ２２ 液面封止部位 ９１ 容器配置板 ９２ 保冷容器 ９３ 保冷剤収容部 Ｃ 冷気 Ｈ （容器配置板の）穴 Ｓ （保冷運搬容器９の）開口部 1 container 2 lid 3 Bottom member 4 Aseptic solution 5 tissue pieces 9 Cold transport container 10 Cooling agent 12 Bottom (of container 1) 22 Liquid level sealing part 91 Container Arrangement Plate 92 Cold storage container 93 Coolant Storage C cold air H (on container placement plate) hole S (for cold storage container 9) opening
フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） Ｂ６５Ｄ 25/10 Ｂ６５Ｄ 25/10 25/20 25/20 Ｒ 77/04 77/04 Ａ 81/18 81/18 Ｂ 81/20 81/20 Ｊ 81/22 81/22 Ｆターム(参考） 2G045 CB01 HA06 HA13 HA14 3E062 AA01 AB07 AC02 CA05 FA02 FB02 FC01 3E067 AA12 AB99 BA01C BA03B BB14B BB14C BC03B BC03C EA32 EB27 EC21 FA04 FB12 FC01 GA02 GA21 GD01 4H011 CA01 CA02 CB05 CB14 CC01 CD06 Continuation of front page (51) Int.Cl. 7 Identification code FI theme code (reference) B65D 25/10 B65D 25/10 25/20 25/20 R 77/04 77/04 A 81/18 81/18 B 81 / 20 81/20 J 81/22 81/22 F-term (reference) 2G045 CB01 HA06 HA13 HA14 3E062 AA01 AB07 AC02 CA05 FA02 FB02 FC01 3E067 AA12 AB99 BA01C BA03B BB14B BB14C BC03B BC03C EA32 EB27 EC21 FA04 GA21 FC01 GA02 GA01 FC01 GA02 FB12 FC01 GA01 CA02 CB05 CB14 CC01 CD06
Claims (7)
収容するための容器であって、前記容器を閉塞する蓋に
は、該容器に所定量収容される溶液の液面を封止する液
面封止部位が設けられていることを特徴とする組織片収
容容器。1. A container for aseptically containing a tissue piece collected from an organism, wherein a lid for closing the container seals a liquid surface of a solution contained in a predetermined amount in the container. A tissue piece accommodating container, which is provided with a liquid surface sealing portion.
とによって、前記液面封止部位が前記液面に接着する状
態と接着しない状態とを適宜選択できるように構成され
たことを特徴とする請求項１に記載の組織片収容容器。2. The liquid level sealing portion can be appropriately selected from a state in which it adheres to the liquid surface and a state in which it does not adhere to the liquid surface by adjusting the degree of fitting of the lid to the container. The tissue piece storage container according to claim 1.
たことを特徴とする請求項１又は請求項２に記載の組織
片容器。3. The tissue piece container according to claim 1, wherein a bottom portion of the container is formed in a substantially conical shape.
の底部材が装着可能とされたことを特徴とする請求項３
に記載の組織片容器。4. The bottom member for mounting the container upright can be attached to the bottom portion.
The tissue piece container described in.
収容するための容器であって、塩類と抗生物質を少なく
とも含有する無菌溶液が、予め所定量、密封収容された
ことを特徴とする組織片収容容器。5. A container for aseptically containing a tissue piece collected from an organism, characterized in that a predetermined amount of a sterile solution containing at least salts and antibiotics is hermetically sealed. A tissue piece container.
のであることを特徴とする請求項１から５に記載の組織
片容器。6. The tissue piece container according to claim 1, wherein the tissue piece is used for regenerative medicine.
組織片収容容器が嵌着できるように形成された穴を複数
備える容器配置板と、 該容器配置板が取り付けられ、保冷機能を有する外容器
と、 該外容器の内側に設けられた保冷剤収容部と、を備え、 前記穴に嵌着された前記組織片容器の組織片収容領域
が、前記保冷剤からの冷気に曝されるように構成された
ことを特徴とする保冷運搬容器。7. A container arrangement plate having a plurality of holes formed so that the tissue piece accommodating container according to any one of claims 1 to 6 can be fitted therein, and the container arrangement plate is attached to the container arrangement plate to provide a cold insulation function. An outer container having an outer container and a cold storage container provided inside the outer container, and a tissue piece storage region of the tissue piece container fitted in the hole is exposed to cool air from the cold storage agent. A cold carrier container characterized by being configured as described above.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002042693A JP2003240775A (en) | 2002-02-20 | 2002-02-20 | Housing container for tissue piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002042693A JP2003240775A (en) | 2002-02-20 | 2002-02-20 | Housing container for tissue piece |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2003240775A true JP2003240775A (en) | 2003-08-27 |
Family
ID=27782710
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002042693A Pending JP2003240775A (en) | 2002-02-20 | 2002-02-20 | Housing container for tissue piece |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2003240775A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008039551A (en) * | 2006-08-04 | 2008-02-21 | Hitachi High-Technologies Corp | Specimen treatment system |
| CN105866368A (en) * | 2016-06-12 | 2016-08-17 | 深圳市清时捷科技有限公司 | Reagent preservation device and method of online water quality analyzer |
| CN105947375A (en) * | 2016-05-31 | 2016-09-21 | 芜湖市佳谷豆工贸有限公司 | Storage box for rice cakes |
| CN109515935A (en) * | 2017-09-20 | 2019-03-26 | 贵港市瑞成科技有限公司 | A kind of living body Sea crab packing box |
| WO2019124303A1 (en) * | 2017-12-18 | 2019-06-27 | テルモ株式会社 | Fragile-object retaining device provided with injection mechanism |
-
2002
- 2002-02-20 JP JP2002042693A patent/JP2003240775A/en active Pending
Cited By (6)
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|---|---|---|---|---|
| JP2008039551A (en) * | 2006-08-04 | 2008-02-21 | Hitachi High-Technologies Corp | Specimen treatment system |
| CN105947375A (en) * | 2016-05-31 | 2016-09-21 | 芜湖市佳谷豆工贸有限公司 | Storage box for rice cakes |
| CN105866368A (en) * | 2016-06-12 | 2016-08-17 | 深圳市清时捷科技有限公司 | Reagent preservation device and method of online water quality analyzer |
| CN105866368B (en) * | 2016-06-12 | 2017-09-26 | 深圳市清时捷科技有限公司 | The reagent save set and its method of a kind of water quality online analyzer |
| CN109515935A (en) * | 2017-09-20 | 2019-03-26 | 贵港市瑞成科技有限公司 | A kind of living body Sea crab packing box |
| WO2019124303A1 (en) * | 2017-12-18 | 2019-06-27 | テルモ株式会社 | Fragile-object retaining device provided with injection mechanism |
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