JP2003227780A - Particle concentrating chip and concentration method for particle using the same - Google Patents
Particle concentrating chip and concentration method for particle using the sameInfo
- Publication number
- JP2003227780A JP2003227780A JP2002025596A JP2002025596A JP2003227780A JP 2003227780 A JP2003227780 A JP 2003227780A JP 2002025596 A JP2002025596 A JP 2002025596A JP 2002025596 A JP2002025596 A JP 2002025596A JP 2003227780 A JP2003227780 A JP 2003227780A
- Authority
- JP
- Japan
- Prior art keywords
- particle
- sample
- flow path
- cleaning liquid
- particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、粒子を濃縮するマ
イクロチップとそれを用いた粒子濃縮方法に関する。TECHNICAL FIELD The present invention relates to a microchip for concentrating particles and a method for concentrating particles using the same.
【０００２】[0002]
【従来の技術】粒子濃度の低い液体試料を濃縮する場
合、大きく分けて２つの方法がある。ひとつは遠心分離
をして上清を除去する方法、他のひとつはフィルタを用
いてろ過する方法である。遠心分離して上清を除去する
方法は、濃縮する粒子が細胞であった場合、細胞にダメ
ージを与えない遠心力で遠心分離する必要があり、時間
を要する。また、ダメージを受けにくい粒子を遠心する
場合においても、遠心後の粒子と液体を分離するために
液体を除去するためデカンテーション等を行う必要があ
り、手間がかかる等の問題点があった。フィルタを用い
てろ過する方法では、粒子が細胞等であった場合、細胞
がフィルタに付着し、細胞を回収する場合において細胞
にダメージを与えてしまうことがあった。また、回収率
が低い等の問題点があった。2. Description of the Related Art There are roughly two methods for concentrating a liquid sample having a low particle concentration. One is a method of removing the supernatant by centrifugation, and the other is a method of filtering using a filter. In the method of centrifuging and removing the supernatant, when the particles to be concentrated are cells, it is necessary to centrifuge with a centrifugal force that does not damage the cells, which requires time. Further, even in the case of centrifuging particles that are not easily damaged, it is necessary to perform decantation or the like in order to remove the liquid in order to separate the liquid from the particles after centrifuging, which is troublesome. In the method of filtering using a filter, when the particles are cells or the like, the cells may adhere to the filter and may damage the cells when the cells are collected. In addition, there were problems such as a low recovery rate.
【０００３】[0003]
【発明が解決しようとする課題】本発明は上記事情に鑑
みてなされたもので、微量であり粒子濃度の低い液体試
料を試料中の細胞などの粒子を傷つけることなく、濃縮
できる粒子濃縮チップとそれを用いた濃縮方法を提供す
ることを課題とするものである。The present invention has been made in view of the above circumstances, and a particle concentration chip capable of concentrating a small amount of a liquid sample having a low particle concentration without damaging particles such as cells in the sample. It is an object to provide a concentration method using the same.
【０００４】[0004]
【課題を解決するための手段】本発明の粒子濃縮チップ
は、粒子捕集部が設けられた第１流路と、前記第１流路
に交差するように設けられた第２流路とを備えたことを
特徴とする。The particle concentrating chip of the present invention comprises a first flow path provided with a particle trap and a second flow path provided so as to intersect with the first flow path. It is characterized by having.
【０００５】また、粒子捕集部が前記第１流路途中に設
けられた複数の支柱であることを特徴とする。粒子捕集
部が前記第１流路途中に設けられた粒子より小さい流径
を持った流路との境界部であっても良い。Further, the particle collecting part is characterized by a plurality of columns provided in the middle of the first flow path. The particle collecting part may be a boundary part with the flow path provided in the middle of the first flow path and having a smaller diameter than the particles.
【０００６】さらに、本発明の粒子濃縮方法は、前記第
１流路に粒子を含む試料を供給する工程と、前記粒子捕
集部で粒子を捕集する工程と、前記粒子捕集部から粒子
を剥離する工程と、前記第２流路に回収液を供給する工
程と、粒子を含む前記回収液を回収する工程からなるこ
とを特徴とする。Further, in the particle concentration method of the present invention, the step of supplying a sample containing particles to the first flow path, the step of collecting particles by the particle collecting section, and the step of collecting particles from the particle collecting section And a step of supplying a recovery liquid to the second flow path, and a step of recovering the recovery liquid containing particles.
【０００７】[0007]
【実施例】以下、図面に示す実施例に基づいてこの発明
を詳述する。これによってこの発明が限定されるもので
はない。DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail with reference to the embodiments shown in the drawings. This does not limit the invention.
【０００８】図１は本発明の粒子濃縮チップDの平面図
である。粒子濃縮チップDの構成について説明する。粒
子濃縮チップDは、試料供給流路５と、前記試料供給流
路５に交差し洗浄液を供給する洗浄液供給流路６、試料
液および洗浄液を排出する排出流路７と、前記排出流路
７に交差し試料中の粒子を回収する回収流路８とを備え
る。試料供給流路５と排出流路７は連通し、ひとつの流
路を形成している。同様に、洗浄液供給流路６と回収流
路８は連通し、ひとつの流路を形成している。試料供給
流路に連通する排出流路７の入口には粒子を捕集するた
めの複数の支柱９が設けられている。この複数の支柱９
は排出流路の入り口に塞ぐように一列に並んでいるが、
これを二重、三重に並べることもできる。また、試料供
給流路５には連通する試料流入口１、洗浄液供給流路６
には連通する洗浄液流入口２、排出流路７には連通する
排出口３、回収流路８には連通する回収口４が設けられ
ている。FIG. 1 is a plan view of the particle concentration chip D of the present invention. The structure of the particle concentration chip D will be described. The particle concentration chip D includes a sample supply flow channel 5, a cleaning liquid supply flow channel 6 that intersects the sample supply flow channel 5 and supplies a cleaning liquid, a discharge flow channel 7 that discharges the sample liquid and the cleaning liquid, and the discharge flow channel 7. And a recovery flow path 8 for recovering particles in the sample. The sample supply channel 5 and the discharge channel 7 communicate with each other to form one channel. Similarly, the cleaning liquid supply channel 6 and the recovery channel 8 communicate with each other to form one channel. A plurality of support columns 9 for collecting particles are provided at the inlet of the discharge channel 7 communicating with the sample supply channel. This plural support 9
Are lined up in a line so as to block the entrance of the discharge channel,
This can be arranged in double or triple. In addition, the sample inlet 1 communicating with the sample supply channel 5 and the cleaning liquid supply channel 6
Is provided with a cleaning liquid inlet 2, which is in communication with the discharge channel 7, a discharge port 3 which is in communication with the discharge channel 7, and a recovery port 4 which is in communication with the recovery channel 8.
【０００９】図２は図1のＡ−Ａ矢視断面図である。こ
の図を用いて粒子濃縮チップＤの構成を説明する。アク
リル樹脂性の基台４１の上部には凹部１５が設けられ、
凹部１５にチップ１０が嵌め込まれている。チップ１０
はガラス板１２と洗浄液流入口２、回収口４、洗浄液供
給流路６、回収流路８を備えるシリコン板１３とから構
成される。基台４１にはチップ１０を押さえる押さえ部
材１１が嵌め込まれている。FIG. 2 is a sectional view taken along the line AA of FIG. The configuration of the particle concentration chip D will be described with reference to this figure. The concave portion 15 is provided on the top of the acrylic resin base 41,
The chip 10 is fitted in the recess 15. Chip 10
Is composed of a glass plate 12, a cleaning liquid inlet 2, a recovery port 4, a cleaning liquid supply channel 6, and a silicon plate 13 having a recovery channel 8. A pressing member 11 for pressing the chip 10 is fitted into the base 41.
【００１０】洗浄液流入口２は連通孔２６を介してチュ
ーブ３６に繋がっている。連通孔２６の上部には凹部２
２が形成されており、ゴム製のＯリング２４が嵌め込ま
れ、洗浄液流入口２の周りから漏水するのを防いでい
る。基台４１には雌ねじ部３４が形成されており、雄ね
じ部３２を形成しているニップル２８が嵌合し、チュー
ブ３６を締め付けて連通孔２６からの漏水を防でいる。The cleaning liquid inlet 2 is connected to a tube 36 via a communication hole 26. The recessed portion 2 is provided above the communication hole 26.
2 is formed, and a rubber O-ring 24 is fitted therein to prevent water from leaking from around the cleaning liquid inlet 2. A female screw portion 34 is formed on the base 41, the nipple 28 forming the male screw portion 32 is fitted thereto, and the tube 36 is tightened to prevent water leakage from the communication hole 26.
【００１１】回収口４は連通孔２５を介してチューブ３
５に繋がっている。連通孔２５の上部には凹部２１が形
成されており、ゴム製のＯリング２３が嵌め込まれ、回
収口４の周りから漏水するのを防いでいる。基台４１に
は雌ねじ部３３が形成されており、雄ねじ部３１を形成
しているニップル２７が嵌合し、チューブ３５を締め付
けて連通孔２５からの漏水を防でいる。The recovery port 4 is connected to the tube 3 through the communication hole 25.
It is connected to 5. A recess 21 is formed in the upper part of the communication hole 25, and a rubber O-ring 23 is fitted therein to prevent water from leaking from around the recovery port 4. A female screw portion 33 is formed on the base 41, the nipple 27 forming the male screw portion 31 is fitted thereto, and the tube 35 is tightened to prevent water leakage from the communication hole 25.
【００１２】チップ１０に形成されている試料供給流路
５、洗浄液供給流路６、排出流路７と回収流路８は幅
１．０ｍｍ×高さ５０μｍであり、試料流入口１から排
出口３まで長さと、洗浄液流入口２から回収口４までの
長さは６．０ｍｍである。支柱９の大きさは縦２５μｍ
×横２５μｍ×高さ５０μｍであり、支柱９間の距離は
１〜５μｍである。試料供給流路５、洗浄液供給流路
６、排出流路７、回収流路８と試料流入口１、洗浄液流
入口２、排出口３、回収口４と複数の支柱９はシリコン
板１３上に半導体製造プロセスを用いたＤeeｐ−RIEに
よる二段階エッチングにより、形成されたものである。The sample supply channel 5, the cleaning liquid supply channel 6, the discharge channel 7 and the recovery channel 8 formed on the chip 10 have a width of 1.0 mm and a height of 50 μm. 3 and the length from the cleaning liquid inflow port 2 to the recovery port 4 are 6.0 mm. The size of the pillar 9 is 25 μm in length
× width 25 μm × height 50 μm, and the distance between the columns 9 is 1 to 5 μm. The sample supply flow channel 5, the cleaning liquid supply flow channel 6, the discharge flow channel 7, the recovery flow channel 8 and the sample flow inlet 1, the cleaning liquid flow inlet 2, the discharge port 3, the recovery port 4 and the plurality of support columns 9 are provided on the silicon plate 13. It is formed by two-step etching by Deep-RIE using a semiconductor manufacturing process.
【００１３】次に、本発明品である粒子濃縮チップを用
いた粒子濃縮方法について説明する。図３は粒子濃縮チ
ップを用いた流体回路図である。まず、洗浄液の吸引工
程について説明する。バルブＳＶ５９をオンにし、シリ
ンジポンプＳＰ７４を吸引動作させることにより、洗浄
液チャンバ１０４からシリンジポンプＳＰ７４のシリン
ジ内に洗浄液を吸引した後、バルブＳＶ５９をオフにす
る。次に、バルブＳＶ５５、５７をオンにし、シリンジ
ポンプＳＰ７２、７３を吸引動作させることにより、洗
浄液チャンバ１０４からシリンジポンプ７２、７３のシ
リンジ内に洗浄液を吸引した後、バルブＳＶ５５、５７
をオフにする。以上の工程により、シリンジポンプＳＰ
７２、７３、７４のシリンジ内に洗浄液が満たされた状
態となる。Next, a particle concentration method using the particle concentration tip of the present invention will be described. FIG. 3 is a fluid circuit diagram using a particle concentration chip. First, the cleaning liquid suction step will be described. The valve SV59 is turned on and the syringe pump SP74 is suctioned to suck the cleaning liquid from the cleaning liquid chamber 104 into the syringe of the syringe pump SP74, and then the valve SV59 is turned off. Next, the valves SV55, 57 are turned on, and the syringe pumps SP72, 73 are suctioned to suck the cleaning liquid from the cleaning liquid chamber 104 into the syringes of the syringe pumps 72, 73, and then the valves SV55, 57.
Turn off. Through the above steps, the syringe pump SP
The cleaning liquid is filled in the syringes 72, 73, and 74.
【００１４】試料の吸引・濃縮工程について説明する。
バルブＳＶ５１をオンにし、シリンジポンプＳＰ７１
を吸引動作させることにより、容器１０１内の試料を試
料吸引ピペットＰからシリンジポンプＳＰ７１のシリン
ジ内に吸引した後、バルブＳＶ５１をオフにする。次
に、バルブＳＶ５３、５４、６２をオンにし、シリンジ
ポンプＳＰ７１を排出動作することにより、シリンジポ
ンプＳＰ７１のシリンジ内の試料は試料流入口１を通し
て試料濃縮チップＤ内に供給される。試料中の粒子は試
料供給流路５を通り、複数の支柱９に捕集される（図
４）。次に、シリンジポンプＳＰ７２を排出動作する
ことにより、シリンジポンプＳＰ７２のシリンジ内の洗
浄液は洗浄液流入口２を通して試料濃縮チップＤ内に供
給される。洗浄液は洗浄液供給流路６、排出流路７、排
出口３を通って、流路内を洗浄した後、排出チャンバ１
０２内に排出される。その後、バルブＳＶ５３、５４を
オフにする。バルブ５５をオンにし、シリンジポンプＳ
Ｐ７２を吸引動作することにより、シリンジポンプＳＰ
７２のシリンジ内に洗浄液を吸引した後、バルブＳＶ５
５をオフにする。、の工程を数回繰り返し、試料の
吸引・濃縮工程を繰り返す。The sample suction / concentration process will be described.
Turn on the valve SV51 and turn on the syringe pump SP71.
By suctioning the sample in the container 101 from the sample suction pipette P into the syringe of the syringe pump SP71, the valve SV51 is turned off. Next, the valves SV53, 54, 62 are turned on, and the syringe pump SP71 is discharged, whereby the sample in the syringe of the syringe pump SP71 is supplied into the sample concentration chip D through the sample inlet 1. Particles in the sample pass through the sample supply channel 5 and are collected by the plurality of columns 9 (FIG. 4). Next, by discharging the syringe pump SP72, the cleaning liquid in the syringe of the syringe pump SP72 is supplied into the sample concentration chip D through the cleaning liquid inlet 2. The cleaning liquid passes through the cleaning liquid supply flow path 6, the discharge flow path 7, and the discharge port 3 to clean the inside of the flow path, and then the discharge chamber 1
It is discharged in 02. After that, the valves SV53, 54 are turned off. Turn on the valve 55 and turn on the syringe pump S.
By suctioning P72, syringe pump SP
After suctioning the cleaning liquid into the 72 syringe, the valve SV5
Turn off 5. The steps of and are repeated several times, and the sample suction / concentration step is repeated.
【００１５】最後の試料の吸引・濃縮工程は、の工程
におけるバルブ動作において、バルブＳＶ５３をオフに
するだけで、シリンジポンプＳＰ７２を吸引動作は行わ
ない。In the final sample suction / concentration step, in the valve operation in the step, only the valve SV53 is turned off, and the syringe pump SP72 is not sucked.
【００１６】試料回収の工程について説明する。シリン
ジポンプＳＰ７２を排出動作することにより、シリンジ
ポンプＳＰ７２のシリンジ内の洗浄液は洗浄液流入口２
を通して試料濃縮チップＤ内に供給される。洗浄液は洗
浄液供給路６、排出流路７、排出口３を通って、流路内
を洗浄した後、排出チャンバ１０２内に排出される。次
に、バルブＳＶ６０、６３をオンにし、バルブＳＶ６２
をオフにする。シリンジポンプＳＰ７３を排出動作する
ことにより、シリンジポンプＳＰ７３のシリンジ内の洗
浄液は排出口３を通して回収流路８を流れ、回収チャン
バ１０３に排出される。シリンジポンプＳＰ７３を一旦
止め、バルブＳＶ６３オフ、オンを繰り返してウォータ
ーハンマーを発生させ、複数の支柱９にトラップされた
粒子を分散させる（図５）。再度、シリンジポンプＳＰ
７３を排出動作することにより、シリンジポンプ３のシ
リンジ内の洗浄液は排出口３を通して回収流路８を流
れ、回収チャンバ１０３に粒子とともに排出される（図
６）。The sample recovery process will be described. By performing the discharging operation of the syringe pump SP72, the cleaning liquid in the syringe of the syringe pump SP72 becomes the cleaning liquid inlet 2
Through the sample concentration chip D. The cleaning liquid passes through the cleaning liquid supply path 6, the discharge flow path 7, and the discharge port 3 to clean the inside of the flow path, and then is discharged into the discharge chamber 102. Next, the valves SV60 and 63 are turned on, and the valve SV62
Turn off. By discharging the syringe pump SP73, the cleaning liquid in the syringe of the syringe pump SP73 flows through the discharge port 3 through the recovery passageway 8 and is discharged to the recovery chamber 103. The syringe pump SP73 is once stopped, the valve SV63 is repeatedly turned off and on to generate a water hammer, and the particles trapped in the plurality of columns 9 are dispersed (FIG. 5). Again, syringe pump SP
By performing the discharge operation of 73, the cleaning liquid in the syringe of the syringe pump 3 flows through the discharge port 3 through the recovery channel 8 and is discharged together with the particles into the recovery chamber 103 (FIG. 6).
【００１７】以上のような濃縮方法によって、濃縮前の
試料は濃度約１０００個／μｌから最終的に濃度１００
００個／μｌに濃縮される。試料中の粒子の回収率は、
９０％以上であり、効率良く回収できる。また、粒子濃
縮チップＤ内を流れる流体を適切に制御することによ
り、ダメージを受け易い細胞などを傷つけることなく回
収が可能である。By the concentration method as described above, the concentration of the sample before concentration is about 1000 pieces / μl to the final concentration of 100.
It is concentrated to 00 cells / μl. The recovery rate of particles in the sample is
It is 90% or more and can be efficiently recovered. Further, by appropriately controlling the fluid flowing in the particle concentrating chip D, it is possible to collect the cells, which are easily damaged, without damaging them.
【００１８】次に、他の実施例について説明する。この
実施例においては、先の実施例と同じ構成要素には同じ
参照番号を付しその説明を省略する。図７は粒子濃縮チ
ップＥの平面図であり、図８は図１のチップ１０のＢ−
Ｂ矢視断面図である。Next, another embodiment will be described. In this embodiment, the same components as those of the previous embodiment are designated by the same reference numerals and the description thereof will be omitted. FIG. 7 is a plan view of the particle concentrating chip E, and FIG. 8 is B- of the chip 10 of FIG.
FIG.
【００１９】排出流路７は、壁８１（境界部）から試料
供給流路５に比べて捕集する粒子より小さい断面積を持
った流路となる。そのため、試料供給流路５から供給さ
れた粒子を含んだ粒子含有液は、排出流路７に入ると
き、粒子のみ壁８１に留まり、捕集される。The discharge channel 7 is a channel having a smaller cross-sectional area than the particles collected from the wall 81 (boundary portion) as compared with the sample supply channel 5. Therefore, when the particle-containing liquid containing particles supplied from the sample supply channel 5 enters the discharge channel 7, only the particles remain on the wall 81 and are collected.
【００２０】チップ１０に形成されている試料供給流路
５、洗浄液供給流路６と回収流路８は幅１．０ｍｍ×高
さ５０μｍ、排出流路７は幅１．０ｍｍ×高さ５μｍで
あり、試料流入口１から排出口３まで長さと、洗浄液流
入口２から回収口４までの長さは６．０ｍｍである。試
料供給流路５、洗浄液供給流路６、排出流路７、回収流
路８と試料流入口１、洗浄液流入口２、排出口３、回収
口４はシリコン板１３上に半導体製造プロセスを用いた
Ｄeeｐ−RIEによる二段階エッチングにより、形成され
たものである。The sample supply channel 5, the cleaning liquid supply channel 6 and the recovery channel 8 formed on the chip 10 have a width of 1.0 mm and a height of 50 μm, and the discharge channel 7 has a width of 1.0 mm and a height of 5 μm. The length from the sample inlet 1 to the outlet 3 and the length from the cleaning liquid inlet 2 to the recovery port 4 are 6.0 mm. The sample supply flow path 5, the cleaning liquid supply flow path 6, the discharge flow path 7, the recovery flow path 8 and the sample inflow port 1, the cleaning liquid inflow port 2, the discharge port 3, and the recovery port 4 are used on the silicon plate 13 for the semiconductor manufacturing process. It was formed by the two-step etching by the Deep-RIE.
【００２１】本実施例の粒子濃縮チップを用いた粒子濃
縮方法は、先の実施例で説明した工程と同じであるた
め、説明は省略する。The method of concentrating particles using the particle concentrating chip of this embodiment is the same as the process described in the previous embodiment, so the explanation is omitted.
【発明の効果】本発明は、微量であり粒子濃度の低い液
体試料を試料中の細胞などの粒子を傷つけることなく、
濃縮できる。INDUSTRIAL APPLICABILITY According to the present invention, a liquid sample having a small amount and a low particle concentration can be used without damaging particles such as cells in the sample.
Can be concentrated.
【図面の簡単な説明】[Brief description of drawings]
【図１】本発明の粒子濃縮チップの平面図である。FIG. 1 is a plan view of a particle concentration chip of the present invention.
【図２】図１のＡ−Ａ矢視断面図である。FIG. 2 is a sectional view taken along the line AA of FIG.
【図３】本発明の粒子濃縮チップを用いた流体回路図で
ある。FIG. 3 is a fluid circuit diagram using the particle concentration chip of the present invention.
【図４】濃縮工程を示した模式図である。FIG. 4 is a schematic diagram showing a concentration step.
【図５】濃縮工程を示した模式図である。FIG. 5 is a schematic diagram showing a concentration step.
【図６】濃縮工程を示した模式図である。FIG. 6 is a schematic diagram showing a concentration step.
【図７】本発明の粒子濃縮チップの平面図である。FIG. 7 is a plan view of the particle concentration chip of the present invention.
【図８】図７のＡ−Ａ矢視断面図の要部である。8 is a main part of a cross-sectional view taken along the line AA of FIG.
１ 試料流入口 ２ 洗浄液流入口 ３ 排出口 ４ 回収口 ５ 試料供給流路 ６ 洗浄液供給流路 ７ 排出流路 ９ 支柱 １０ チップ １１ 押さえ部材 １２ ガラス板 １３ シリコン板 １５ 凹部 ２１、２２ 凹部 ２３、２４ Ｏリング ２５、２６ 連通部 ２７、２８ ニップル ３１、３２ 雄ねじ部 ３３、３４ 雌ねじ部 ３５、３６ チューブ ４１ 基台 1 Sample inlet 2 Cleaning liquid inlet 3 outlets 4 Collection port 5 Sample supply channel 6 Cleaning liquid supply channel 7 discharge channel 9 props 10 chips 11 Holding member 12 glass plates 13 Silicon plate 15 recess 21, 22 recess 23, 24 O-ring 25, 26 Communication part 27, 28 nipples 31, 32 Male thread 33, 34 Female thread part 35, 36 tubes 41 base
───────────────────────────────────────────────────── フロントページの続き Ｆターム(参考） 2G052 AA33 AD34 CA03 CA20 CA29 CA35 DA09 EA05 ED11 FC07 FC11 JA13 JA16 ─────────────────────────────────────────────────── ─── Continued front page F term (reference) 2G052 AA33 AD34 CA03 CA20 CA29 CA35 DA09 EA05 ED11 FC07 FC11 JA13 JA16
Claims (4)
記第１流路に交差するように設けられた第２流路と、を
備えたことを特徴とする粒子濃縮チップ。1. A particle concentration chip, comprising: a first flow path provided with a particle collecting section; and a second flow path provided so as to intersect with the first flow path.
れた複数の支柱であることを特徴とする請求項１記載の
粒子濃縮チップ。2. The particle concentration chip according to claim 1, wherein the particle collecting section is a plurality of columns provided in the middle of the first flow path.
れた粒子より小さい流径を持った流路との境界部である
ことを特徴とする請求項１記載の粒子濃縮チップ。3. The particle concentration chip according to claim 1, wherein the particle collecting part is a boundary part with a flow path provided in the middle of the first flow path and having a flow diameter smaller than that of the particles.
る工程と、前記粒子捕集部で粒子を捕集する工程と、前
記粒子捕集部から粒子を剥離する工程と、前記第２流路
に回収液を供給する工程と、粒子を含む前記回収液を回
収する工程からなることを特徴とする粒子濃縮方法。4. A step of supplying a sample containing particles to the first flow path, a step of collecting particles by the particle collecting section, a step of separating particles from the particle collecting section, A method for concentrating particles, comprising a step of supplying a recovery liquid to two channels and a step of recovering the recovery liquid containing particles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002025596A JP2003227780A (en) | 2002-02-01 | 2002-02-01 | Particle concentrating chip and concentration method for particle using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002025596A JP2003227780A (en) | 2002-02-01 | 2002-02-01 | Particle concentrating chip and concentration method for particle using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003227780A true JP2003227780A (en) | 2003-08-15 |
| JP2003227780A5 JP2003227780A5 (en) | 2005-08-18 |
Family
ID=27747706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002025596A Pending JP2003227780A (en) | 2002-02-01 | 2002-02-01 | Particle concentrating chip and concentration method for particle using the same |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7670561B2 (en) | 2005-05-13 | 2010-03-02 | Hitachi Plant Technologies, Ltd. | Micro fluidic device and joint therefor |
| WO2010061201A2 (en) * | 2008-11-26 | 2010-06-03 | Ucl Business Plc | Microfluidic device |
| WO2011034978A3 (en) * | 2009-09-17 | 2011-07-28 | Innovaprep Llc | Liquid to liquid biological particle concentrator with disposable fluid path |
| US8462332B2 (en) | 2008-08-21 | 2013-06-11 | Siemens Healthcare Diagnostics, Inc. | Multi-layer slides for analysis of urine sediments |
| JP2015014512A (en) * | 2013-07-04 | 2015-01-22 | 住友ゴム工業株式会社 | Micro flow channel chip and medical measurement instrument |
| JP2018205047A (en) * | 2017-05-31 | 2018-12-27 | シスメックス株式会社 | Specimen processing chip, liquid feeder of specimen processing chip, and liquid feeding method |
-
2002
- 2002-02-01 JP JP2002025596A patent/JP2003227780A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7670561B2 (en) | 2005-05-13 | 2010-03-02 | Hitachi Plant Technologies, Ltd. | Micro fluidic device and joint therefor |
| US8462332B2 (en) | 2008-08-21 | 2013-06-11 | Siemens Healthcare Diagnostics, Inc. | Multi-layer slides for analysis of urine sediments |
| WO2010061201A2 (en) * | 2008-11-26 | 2010-06-03 | Ucl Business Plc | Microfluidic device |
| WO2010061201A3 (en) * | 2008-11-26 | 2010-09-10 | Ucl Business Plc | Microfluidic device |
| US11033897B2 (en) | 2008-11-26 | 2021-06-15 | Ucl Business Plc | Microfluidic device |
| WO2011034978A3 (en) * | 2009-09-17 | 2011-07-28 | Innovaprep Llc | Liquid to liquid biological particle concentrator with disposable fluid path |
| JP2015014512A (en) * | 2013-07-04 | 2015-01-22 | 住友ゴム工業株式会社 | Micro flow channel chip and medical measurement instrument |
| JP2018205047A (en) * | 2017-05-31 | 2018-12-27 | シスメックス株式会社 | Specimen processing chip, liquid feeder of specimen processing chip, and liquid feeding method |
| JP7010603B2 (en) | 2017-05-31 | 2022-01-26 | シスメックス株式会社 | Specimen processing chip |
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