JP2003126883A - Decomposition treatment process of persistent pollutants in wastewater - Google Patents
Decomposition treatment process of persistent pollutants in wastewaterInfo
- Publication number
- JP2003126883A JP2003126883A JP2001326123A JP2001326123A JP2003126883A JP 2003126883 A JP2003126883 A JP 2003126883A JP 2001326123 A JP2001326123 A JP 2001326123A JP 2001326123 A JP2001326123 A JP 2001326123A JP 2003126883 A JP2003126883 A JP 2003126883A
- Authority
- JP
- Japan
- Prior art keywords
- wastewater
- substance
- decomposing
- substances
- decomposable substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Biological Treatment Of Waste Water (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Activated Sludge Processes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】この発明は下水処理、し尿処
理、浸出液排水処理、小規模下水処理等における排水処
理方法に関し、更に詳細には、白色腐朽菌や糸状菌など
の難分解性物質分解菌を利用してダイオキシン類や外因
性内分泌撹乱物質（以下、環境ホルモンと云う）などの
難分解性物質を分解処理する排水中の難分解性物質の分
解処理方法に関する。TECHNICAL FIELD The present invention relates to a wastewater treatment method in sewage treatment, night soil treatment, leachate wastewater treatment, small-scale sewage treatment, and the like, and more specifically, to microorganisms that decompose persistent substances such as white-rot fungi and filamentous fungi. The present invention relates to a method for decomposing a hardly decomposable substance in wastewater, which decomposes a hardly decomposable substance such as dioxins and exogenous endocrine disrupting substances (hereinafter referred to as environmental hormones).
【０００２】[0002]
【従来の技術】一般に、下水処理・し尿処理・浸出液排
水処理・小規模下水処理などの排水中には広範囲の有機
物および無機物が含まれており、この中でも近年特に注
目されているダイオキシン類や環境ホルモンなどの難分
解性物質の除去は、従来から使用されている生物反応除
去設備では困難であった。2. Description of the Related Art Generally, a wide range of organic substances and inorganic substances are contained in wastewater such as sewage treatment, human waste treatment, leachate wastewater treatment, and small-scale sewage treatment. Removal of persistent substances such as hormones has been difficult with conventional biological reaction removal equipment.
【０００３】従来の廃水処理方法には、通常の下水処理
において用いられる活性汚泥法、この活性汚泥法の全プ
ロセスを１つの完全混合反応槽で行う回分式活性汚泥
法、また微生物を樹脂材に固定して排水を通水浄化する
生物膜法等がある。これらの生物処理設備では、水溶性
・低重合度・低分子量等の易分解性物質の分解が限界
で、難分解性物質を分解することは困難であった。The conventional wastewater treatment methods include an activated sludge method used in ordinary sewage treatment, a batch activated sludge method in which all processes of this activated sludge method are carried out in one complete mixing reaction tank, and microorganisms are used as resin materials. There is a biofilm method, etc., in which the water is purified by passing it through fixed water. In these biological treatment facilities, decomposition of easily decomposable substances such as water solubility, low degree of polymerization, and low molecular weight is limited, and it is difficult to decompose hardly decomposable substances.
【０００４】難分解性物質としては色度成分と言われる
多くの有色物質が含まれるが、近年特に注目されている
のはダイオキシン類や環境ホルモンである。従来の生物
処理で難分解性物質の除去が困難であるのは、難分解性
物質はその分子間力が極めて強固であり、しかもこれら
に対する分解酵素を有しない微生物が多いためでもあ
る。[0004] As the hardly decomposable substance, many colored substances called chromaticity components are included, but dioxins and environmental hormones have received particular attention in recent years. The reason why it is difficult to remove the hardly decomposable substance by the conventional biological treatment is that the intermolecular force of the hardly decomposable substance is extremely strong and many microorganisms do not have a decomposing enzyme for these substances.
【０００５】ダイオキシン類は猛毒物質で、発ガン性、
変異原性、生殖阻害作用など様々な毒性を有している。
ダイオキシン類は固形物に吸着しやすい性質を有するか
ら、排水の逆浸透膜処理によって分離除去することはで
きる。しかし、この方法はダイオキシン類を濃縮するだ
けで、分解して無害化するものではない。逆に、濃縮さ
れたダイオキシン類を含む廃棄物の処理が問題となる。Dioxins are highly toxic substances, carcinogenic,
It has various toxicities such as mutagenicity and reproductive inhibition.
Since dioxins have a property of being easily adsorbed on solid matter, they can be separated and removed by reverse osmosis membrane treatment of waste water. However, this method only concentrates dioxins and does not decompose them to render them harmless. On the contrary, the treatment of waste containing concentrated dioxins becomes a problem.
【０００６】環境ホルモンは、生物や人の内分泌系を撹
乱する物質として近年知られるようになった。この環境
ホルモンも逆浸透膜処理によって効率的に分離できるこ
とが分かっているが、ダイオキシン類と同様に濃縮排水
の処理が問題となってくる。また、分解処理法について
は種々の研究がされているが、期待される結果が得られ
ているとは言い難い。Environmental hormones have become known in recent years as substances that disturb the endocrine system of organisms and humans. It is known that this environmental hormone can also be efficiently separated by reverse osmosis membrane treatment, but the treatment of concentrated wastewater becomes a problem, as with dioxins. Moreover, although various studies have been conducted on the decomposition treatment method, it is hard to say that the expected results have been obtained.
【０００７】そこで、ダイオキシン類や環境ホルモン等
の難分解性物質を含有した排水を処理するために、主に
用いられている方法は、促進酸化法と呼ばれる物理化学
的処理法である。この方法では、オゾンや過酸化水素や
紫外線により強力な酸化剤であるＯＨラジカルを発生さ
せ、このＯＨラジカルによって難分解性物質を酸化分解
しようとするものである。Therefore, a method mainly used for treating wastewater containing hardly decomposable substances such as dioxins and environmental hormones is a physicochemical treatment method called an accelerated oxidation method. In this method, OH radicals which are strong oxidants are generated by ozone, hydrogen peroxide or ultraviolet rays, and the OH radicals try to oxidize and decompose the hardly decomposable substance.
【０００８】[0008]
【発明が解決しようとする課題】しかし、オゾンや過酸
化水素は薬品代が高価であるばかりでなく、生物に対す
る毒性が極めて強いと言われている。オゾンは変異原性
を有するとともに発ガン性化合物の生成に関与すること
が知られており、同様に過酸化水素も発ガン性が報告さ
れている。However, it is said that ozone and hydrogen peroxide are not only expensive for chemicals but also extremely toxic to living organisms. It is known that ozone has mutagenicity and is involved in the formation of carcinogenic compounds, and hydrogen peroxide is also reported to be carcinogenic.
【０００９】紫外線による分解除去法では、排水中に懸
濁物質や色度成分（有色物質）があると紫外線の進入が
遮断されるため、紫外線が排水の内部に透過し難くな
り、難分解性物質に対する分解作用が急激に低下する。In the decomposition and removal method using ultraviolet rays, if suspended matter or chromaticity components (colored substances) are contained in the wastewater, the penetration of the ultraviolet rays is blocked, so that it becomes difficult for the ultraviolet rays to permeate the inside of the wastewater, which is difficult to decompose. The decomposition effect on the substance decreases sharply.
【００１０】更に、オゾン発生装置や紫外線照射装置は
設備費用が高価であるため、排水処理施設の通常設備と
して設けるには多額の費用を必要とする。また、これら
装置の運転には多量のエネルギーを必要とし、ランニン
グコストが高価になるという弱点もある。Further, since the equipment cost of the ozone generator and the ultraviolet irradiation device is high, a large amount of cost is required to install the ozone generator and the ultraviolet irradiation device as normal equipment of the wastewater treatment facility. In addition, there is a weak point that a large amount of energy is required to operate these devices and the running cost becomes high.
【００１１】また、他の物理化学的処理法として、排水
中にポリアクリルアミドやＰＡＣ（ポリアルミニウムク
ロライド）等の凝集剤を投入する凝集沈殿法がある。し
かし、ポリアクリルアミドは凝集能の点で優れているも
のの、この物質自身が強い変異原性を示す。特に、ポリ
アクリルアミド中に含まれるモノマーのアクリルアミド
に発ガン性・神経毒があることからその残留性が危惧さ
れている。また、ＰＡＣもアルツハイマー病の発現物質
としてその毒性が指摘されている。Further, as another physicochemical treatment method, there is a coagulation-precipitation method in which a coagulant such as polyacrylamide or PAC (polyaluminum chloride) is added to waste water. However, although polyacrylamide is excellent in aggregating ability, this substance itself exhibits strong mutagenicity. In particular, the residual acrylamide is concerned because acrylamide, a monomer contained in polyacrylamide, has carcinogenicity and neurotoxicity. In addition, PAC has been pointed out as its toxicity as a substance expressing Alzheimer's disease.
【００１２】従って、本発明はダイオキシン類や環境ホ
ルモン等の難分解性物質を分解する菌を利用して、排水
中の難分解性物質を生物処理により炭酸ガス及び易分解
性物質にまで分解して無害化し、この易分解性物質を通
常処理を通して除去することにより、低エネルギーで環
境負荷が小さく、しかも安全な排水中の難分解性物質の
分解処理方法を提供することを目的とする。Therefore, the present invention utilizes a bacterium that decomposes hardly-decomposable substances such as dioxins and environmental hormones to decompose the hardly-decomposable substances in wastewater into carbon dioxide and easily decomposable substances by biological treatment. The purpose of the present invention is to provide a method for decomposing a hardly decomposable substance in wastewater which is low energy, has a low environmental load, and is safe by removing the easily decomposable substance through normal treatment.
【００１３】[0013]
【課題を解決するための手段】請求項１の発明は、バイ
オリアクターに配置された難分解性物質を分解する菌株
により排水に含有される難分解性物質を炭酸ガス及び易
分解性物質にまで分解する工程と、前記バイオリアクタ
ーから排出されるリアクター水を膜分離して高分子成分
及び固体成分をバイオリアクターに還流させ液体成分と
易分解性物質からなる排水を透過排出する工程と、この
膜透過水を易分解性物質処理槽に供給して易分解性物質
を分解処理し清澄な処理水を放出することを特徴とする
排水中の難分解性物質の分解処理方法である。According to the invention of claim 1, the hardly decomposable substance contained in the wastewater by the strain which decomposes the hardly decomposable substance arranged in the bioreactor is converted into carbon dioxide gas and easily decomposable substance. A step of decomposing, a step of separating the reactor water discharged from the bioreactor into membranes, refluxing a polymer component and a solid component into the bioreactor, and permeating and discharging a wastewater composed of a liquid component and a readily decomposable substance, and this membrane It is a method for decomposing a hardly decomposable substance in wastewater, which comprises supplying permeated water to a tank for easily decomposing a substance to decompose the easily decomposable substance and releasing clear treated water.
【００１４】請求項２の発明は、前記難分解性物質を分
解する菌株が白色腐朽菌又は糸状菌である請求項１に記
載の排水中の難分解性物質の分解処理方法である。A second aspect of the present invention is the method for decomposing the persistent substance in wastewater according to claim 1, wherein the strain decomposing the persistent substance is a white rot fungus or a filamentous fungus.
【００１５】請求項３の発明は、前記難分解性物質がダ
イオキシン類又は外因性内分泌撹乱物質である請求項１
又は２に記載の排水中の難分解性物質の分解処理方法で
ある。In the invention of claim 3, the hardly decomposable substance is a dioxin or an exogenous endocrine disrupting substance.
Alternatively, the method for decomposing and processing hardly-decomposable substances in wastewater according to the above item 2.
【００１６】請求項４の発明は、難分解性物質を分解す
る菌株を担体に固定した微生物固定担体を前記バイオリ
アクターに配置する請求項１に記載の排水中の難分解性
物質の分解処理方法である。[0016] The invention according to claim 4 is a method for decomposing a hardly-decomposable substance in wastewater according to claim 1, wherein a microorganism-immobilized carrier in which a strain that decomposes the hardly-decomposable substance is immobilized on the carrier is arranged in the bioreactor. Is.
【００１７】請求項５の発明は、前記易分解性物質処理
槽は生物膜処理槽である請求項１に記載の排水中の難分
解性物質の分解処理方法である。A fifth aspect of the present invention is the method for decomposing a hardly decomposable substance in wastewater according to the first aspect, wherein the easily degradable substance treatment tank is a biofilm treatment tank.
【００１８】[0018]
【発明の実施の形態】本発明は排水中の難分解性物質を
微生物を利用して分解し、有害なダイオキシン類や環境
ホルモン等を無害化処理することを意図したものであ
る。難分解性物質を微生物分解した後に得られる易分解
性物質の除去には、活性汚泥法・固定化微生物法・生物
膜処理法などの通常処理法を利用する。易分解性物質の
処理に生物分解法を適用すれば、難分解性物質を一貫し
た生物処理により無害化する排水処理方法を実現でき
る。BEST MODE FOR CARRYING OUT THE INVENTION The present invention is intended to detoxify harmful dioxins, environmental hormones, etc. by decomposing hardly-decomposable substances in wastewater using microorganisms. In order to remove easily degradable substances obtained by microbial decomposition of hardly decomposable substances, ordinary treatment methods such as activated sludge method, immobilized microorganism method, biofilm treatment method and the like are used. If the biodegradation method is applied to the treatment of easily decomposable substances, it is possible to realize a wastewater treatment method that makes harmless degradable substances harmless by consistent biological treatment.
【００１９】この方式は、微生物の生物活性を利用する
ため、簡単な設備で効率よく汚濁物質を除去することが
可能となる。排水中に含有される難分解性物質、特に有
害なダイオキシン類や環境ホルモン等を微生物を利用し
て分解処理することが可能となれば、安価な省エネルギ
ーシステムの構築が可能となる。Since this method utilizes the biological activity of microorganisms, it becomes possible to remove pollutants efficiently with simple equipment. If it becomes possible to decompose and decompose hardly-decomposable substances contained in wastewater, particularly harmful dioxins and environmental hormones, by using microorganisms, an inexpensive energy-saving system can be constructed.
【００２０】前述したように、本発明のターゲットとな
る難分解性物質の一つにダイオキシン類がある。本来、
ダイオキシン類はポリ塩化ジベンゾパラジオキシン及び
ポリ塩化ジベンゾフランの総称であるが、同様な毒性を
示すコプラナーＰＣＢｓをも含めた概念として取り扱わ
れている。ＰＣＢはポリ塩化ビフェニールであり、コプ
ラナーＰＣＢは扁平構造を持つＰＣＢを表す。As mentioned above, dioxins are one of the hardly decomposable substances which is the target of the present invention. Originally
Dioxins are a general term for polychlorinated dibenzoparadioxin and polychlorinated dibenzofuran, but they are treated as a concept including coplanar PCBs showing similar toxicity. PCB is polychlorinated biphenyl and coplanar PCB represents a PCB with a flat structure.
【００２１】ダイオキシン類は多数の異性体を包含して
おり、具体的には、2,3,7,8-TCDD、1,2,3,7,8-PeCDD、
1,2,3,4,7,8-HxCDD、1,2,3,6,7,8-HxCDD、1,2,3,7,8,9-
HxCDD、1,2,3,4,6,7,8-HpCDD、OCDD、2,3,7,8-TCDF、1,
2,3,7,8-PeCDF、2,3,4,7,8-PeCDF、1,2,3,4,7,8-HxCD
F、1,2,3,6,7,8-HxCDF、1,2,3,4,6,7,8-HpCDF等があ
る。Dioxins include many isomers, and specifically, 2,3,7,8-TCDD, 1,2,3,7,8-PeCDD,
1,2,3,4,7,8-HxCDD, 1,2,3,6,7,8-HxCDD, 1,2,3,7,8,9-
HxCDD, 1,2,3,4,6,7,8-HpCDD, OCDD, 2,3,7,8-TCDF, 1,
2,3,7,8-PeCDF, 2,3,4,7,8-PeCDF, 1,2,3,4,7,8-HxCD
F, 1,2,3,6,7,8-HxCDF, 1,2,3,4,6,7,8-HpCDF and the like.
【００２２】また、コプラナーＰＣＢには、3,4,4',5-T
CB、3,3',4,4'-TCB、3,3',4,4',5-PeCB、3,3',4,4',5,
5'-HxCB、2,3,3',4,4'-PeCB、2,3,4,4',5-PeCB、2,3,
3',4,4',5-HxCB、2,3,3',4,4',5,5'-HpCB等がある。The coplanar PCB also contains 3,4,4 ', 5-T
CB, 3,3 ', 4,4'-TCB, 3,3', 4,4 ', 5-PeCB, 3,3', 4,4 ', 5,
5'-HxCB, 2,3,3 ', 4,4'-PeCB, 2,3,4,4', 5-PeCB, 2,3,
3 ', 4,4', 5-HxCB, 2,3,3 ', 4,4', 5,5'-HpCB, etc.
【００２３】最も毒性の強い2,3,7,8-TCDDの毒性を１と
して、夫々の異性体の毒性等価係数が決められており、
各異性体の量と毒性等価係数の積の合計から毒性等量
（ＴＥＱ：Toxic Equvalent）が算出される。ダイオキ
シン類対策特別措置法によりダイオキシン類の水質の環
境基準が定められ、１Ｌ（リットル）あたりについて１
ｐｇ−ＴＥＱ／Ｌとなっている。本発明の排水処理にお
いてもダイオキシン類の分解除去の目標値としてこの環
境基準値を採用する。With the toxicity of 2,3,7,8-TCDD having the highest toxicity as 1, the toxicity equivalent coefficient of each isomer is determined,
Toxic Equivalent (TEQ) is calculated from the sum of the product of the amount of each isomer and the toxicity equivalent coefficient. Environmental standard for water quality of dioxins is established by the Act on Special Measures Against Dioxins, and 1 per 1 liter (liter)
It is pg-TEQ / L. Also in the wastewater treatment of the present invention, this environmental standard value is adopted as a target value for decomposing and removing dioxins.
【００２４】また、本発明の第２のターゲットとなる難
分解性物質の一つに環境ホルモンがある。前述したよう
に、環境ホルモンは動物の生体内に取り込まれた場合
に、本来、その生体内で営まれている正常なホルモン作
用に影響を及ぼす外因性の物質を意味する。その意味
で、環境ホルモンの正式名称は外因性内分泌撹乱化学物
質と呼ばれる。An environmental hormone is one of the persistent substances which is the second target of the present invention. As described above, the environmental hormone means an exogenous substance that, when taken into the living body of an animal, originally affects the normal hormonal action performed in the living body. In that sense, the official name of environmental hormones is called exogenous endocrine disrupting chemicals.
【００２５】環境ホルモンとして疑われている物質は現
在のところ約７０ある。例えば、ダイオキシン類、ポリ
塩化ビフェニール類（ＰＣＢ）、ポリ臭化ビフェニール
類（ＰＢＢ）、ヘキサクロロベンゼン（ＨＣＢ）、ペン
タクロロフェノール（ＰＣＰ）、アミトロール、クロル
デン、ＤＤＴ、ニトロフェン、トキサフェン、トリブチ
ルスズ、トリフェニルスズ、アルキルフェノール、ノニ
ルフェノール、４オクチルフェノール、ビスフェノール
Ａ、ベンゾフェノン等がある。There are currently about 70 substances suspected as environmental hormones. For example, dioxins, polychlorinated biphenyls (PCB), polybrominated biphenyls (PBB), hexachlorobenzene (HCB), pentachlorophenol (PCP), amitrole, chlordane, DDT, nitrophene, toxaphene, tributyltin, triphenyltin. , Alkylphenol, nonylphenol, 4-octylphenol, bisphenol A, benzophenone and the like.
【００２６】これらの環境ホルモンを規制する法律は広
範囲にわたり、その内４０種類以上は農薬の有効成分で
あるから「農薬取締法」により規制されている。また、
ＰＣＢ、ＤＤＨ、ＨＣＢ、クロルデン等は「化学物質の
審査及び製造等の規制に関する法律」で規制され、ダイ
オキシン類及びフラン類は「大気汚染防止法」・「廃棄
物の処理及び清掃に関する法律」で規制され、ビスフェ
ノールＡは「食品衛生法」で規制されている。The laws for controlling these environmental hormones are wide-ranging, and more than 40 kinds of them are active ingredients of agricultural chemicals, and thus are regulated by the "Agricultural Chemicals Control Law". Also,
PCBs, DDH, HCB, chlordane, etc. are regulated by "Law concerning regulation of examination and manufacturing of chemical substances", and dioxins and furans are regulated by "Air Pollution Control Act" and "Law concerning waste treatment and cleaning" Regulated, bisphenol A is regulated by "Food Sanitation Law".
【００２７】しかし、報告された異常と原因物質との因
果関係や、異常が発生するメカニズム等に関しては十分
明らかにされていないのが現状である。その結果、これ
らの環境ホルモンに対する環境基準は現在のところまだ
規定されておらず、本発明が対象とする排水中の基準量
も明示されていない。従って、本発明では、環境ホルモ
ンの基準量についてはできるだけ濃度を低下させること
が重要であるという観点で検討する。However, at present, the causal relationship between the reported abnormality and the causative substance, the mechanism of occurrence of the abnormality, etc. have not been sufficiently clarified. As a result, environmental standards for these endocrine disrupters have not yet been stipulated at present, nor have the standard amounts in the wastewater targeted by the present invention been specified. Therefore, in the present invention, it is considered from the viewpoint that it is important to reduce the concentration of the endocrine disrupter as much as possible.
【００２８】本発明者等は排水中の難分解性物質、特に
ダイオキシン類や環境ホルモン等の有害物質を分解する
微生物を鋭意研究した結果、カビ菌の一種である白色腐
朽菌や糸状菌の一部が本発明の目的を達成できることを
見出すに至った。The inventors of the present invention have conducted extensive studies on microorganisms that decompose persistent substances in wastewater, particularly dioxins and environmental hormones and other harmful substances. As a result, one of mold fungi, white-rot fungi and filamentous fungi It has been found that the department can achieve the objects of the invention.
【００２９】本発明で用いる白色腐朽菌とは担子菌類に
属し、一般的に木材を腐朽させる担子菌のうちセルロー
ス・リグニンを強力に分解する能力を持つ担子菌のこと
である。具体的にはトゥラメテス属（Ｔｒａｍｅｔｅ
ｓ）、レンツィテス・ベツリナ（Ｌｅｎｚｉｔｅｓ ｂ
ｅｔｕｌｉｎａ）、プレウロトゥス・オストゥレアトゥ
ス（Ｐｌｅｕｒｏｔｕｓ ｏｓｔｒｅａｔｕｓ）等の好
気性菌が存し、特にアラゲカワラタケとも云われるトゥ
ラメテス・ヒルスタ（Ｔｒａｍｅｔｅｓ ｈｉｒｓｕｔ
ａ）は、本発明にとって好ましい白色腐朽菌である。The white-rot fungi used in the present invention belong to basidiomycetes, and are basidiomycetes having a capability of strongly degrading cellulose lignin among basidiomycetes which generally cause wood decay. Specifically, the genus Trametes
s), Lenzites b.
etulina), Pleulotus ostreatus, and other aerobic bacteria, and in particular, Turametes hirsut, also referred to as Arakawakawatake
a) is a preferred white-rot fungus for the present invention.
【００３０】また、難分解性物質分解菌として、白色腐
朽菌と同様の性質を示す糸状菌がある。その中でもクラ
ドスポリウム属（Ｃｌａｄｏｓｐｏｒｉｕｍ）の糸状菌
は、色度成分などの難分解性物質を強力に分解できるこ
とが分かった。Further, as a microorganism decomposing a hardly decomposable substance, there is a filamentous fungus having the same properties as the white-rot fungus. Among them, it has been found that filamentous fungi of the genus Cladosporium can strongly decompose persistent substances such as chromaticity components.
【００３１】白色腐朽菌が菌体外に放出する酵素には、
リグニンペルオキシダーゼ、マンガンペルオキシダー
ゼ、ラッカーゼ等がある。リグニンペルオキシダーゼや
マンガンペルオキシダーゼは過酸化水素分解酵素で、白
色腐朽菌が生成する過酸化水素はこれらの酵素により分
解されてＯＨラジカルになる。このＯＨラジカルは強力
な酸化作用を有し、ダイオキシン類・環境ホルモン・色
度成分等の難分解性物質を炭酸ガス及び易分解性物質に
まで酸化分解する。The enzymes released by the white-rot fungi outside the cells include
Examples include lignin peroxidase, manganese peroxidase, and laccase. Lignin peroxidase and manganese peroxidase are hydrogen peroxide-degrading enzymes, and hydrogen peroxide produced by white-rot fungi is decomposed by these enzymes into OH radicals. The OH radical has a strong oxidizing action, and oxidizes and decomposes hardly decomposable substances such as dioxins, environmental hormones, and chromaticity components into carbon dioxide gas and easily decomposable substances.
【００３２】ラッカーゼはフェノール性水酸基を酸化す
る酵素で、例えばヒドロキノン、ポリフェノール、ｐ−
フェニレンジアミン、アスコルビン酸、シアニン色素な
どを酸化し、その作用はＣＯでは阻害されない。この酸
化作用により、ベンゼン環を切断して分解することがで
きる。従って、ベンゼン環から構成されるダイオキシン
類に対しては顕著な分解効果を発揮する。Laccase is an enzyme that oxidizes phenolic hydroxyl groups, such as hydroquinone, polyphenol and p-.
It oxidizes phenylenediamine, ascorbic acid, cyanine dyes, etc., and its action is not inhibited by CO. Due to this oxidizing action, the benzene ring can be cleaved and decomposed. Therefore, it exerts a remarkable decomposition effect on dioxins composed of benzene rings.
【００３３】実排水処理では、難分解性物質分解菌をバ
イオリアクター内において培養するため、栄養源となる
有機物の含量が少ない場合には、炭素源としてグルコー
ス、スクロース、フルクトース等の糖類や、アルコール
類、有機酸を所定量添加することが望ましい。また、他
の雑菌の繁殖を防止するために、実排水を酸性に設定す
るｐＨ調整剤を添加する。このｐＨ調整剤としては、塩
酸などの強酸や酢酸などの有機酸など広範囲の酸が利用
される。In the actual wastewater treatment, the hardly decomposing substance-degrading bacteria are cultured in the bioreactor. Therefore, when the content of organic substances as nutrient sources is small, sugars such as glucose, sucrose, and fructose as carbon sources, and alcohol. It is desirable to add a certain amount of the organic acids. Moreover, in order to prevent the propagation of other miscellaneous bacteria, a pH adjusting agent that sets the actual wastewater to be acidic is added. A wide range of acids such as strong acids such as hydrochloric acid and organic acids such as acetic acid are used as the pH adjuster.
【００３４】本発明は、バイオリアクターに配置された
難分解性物質を分解する菌体により排水に含有される難
分解性物質を炭酸ガス及び易分解性物質にまで分解する
工程と、前記バイオリアクターから排出されるリアクタ
ー水を膜分離して高分子成分及び固体成分をバイオリア
クターに還流させ液体成分と易分解性物質からなる排水
を選択的に透過排出する工程と、この膜透過水を易分解
性物質処理槽に供給して易分解性物質を分解処理する工
程から構成されている。The present invention comprises a step of decomposing a hardly decomposable substance contained in wastewater into carbon dioxide gas and an easily decomposable substance by a microbial cell that decomposes the hardly decomposable substance arranged in the bioreactor, and the bioreactor. Reactor water discharged from the membrane is separated into membranes and polymer components and solid components are returned to the bioreactor to selectively permeate and discharge wastewater consisting of liquid components and easily degradable substances, and the membrane permeated water is easily decomposed. It is composed of a process of decomposing an easily decomposable substance by supplying it to a volatile substance treatment tank.
【００３５】事業所や家庭などから発生する下水・し尿
・浸出液などの1次排水には、砂や無機物などの固形成
分や易分解性物質や難分解性物質が含まれている。従っ
て、砂や無機成分を除去するために、砂沈殿槽により1
次排水から砂成分を除去し、また凝集剤を投入して浮遊
無機成分を凝集沈殿させる。その後、活性汚泥法により
易分解性有機成分を分解除去して、1次排水を清澄な２
次排水に変化させる。Primary effluents such as sewage, night soil, leachate, etc. generated from business establishments and homes contain solid components such as sand and inorganic substances, easily decomposable substances and hardly decomposable substances. Therefore, in order to remove sand and inorganic components, 1
The sand component is removed from the next waste water, and a flocculant is added to coagulate and settle the suspended inorganic components. After that, the easily degradable organic components are decomposed and removed by the activated sludge method, and the primary wastewater is clarified.
Change to the next drainage.
【００３６】しかし、この２次排水には前記通常処理に
よっては除去しきれなかった難分解性物質がまだ含有さ
れている。この残留する難分解性物質が、前述したダイ
オキシン類・環境ホルモン・色度成分などである。本発
明はこの２次排水に適用されるとその効果が大きい。However, this secondary waste water still contains a hardly decomposable substance which cannot be completely removed by the above-mentioned normal treatment. The remaining hardly decomposable substances are the above-mentioned dioxins, environmental hormones, and chromaticity components. When the present invention is applied to this secondary drainage, its effect is great.
【００３７】第１工程のバイオリアクターでは、難分解
性物質分解菌を排水で培養するだけでよく、その結果、
菌体外に難分解性物質分解酵素が放出され、この分解酵
素により難分解性物質が炭酸ガス及び易分解性物質にま
で分解されてゆく。その結果、高分子の難分解性物質が
低分子の易分解性物質に変化し、次工程の分離膜を透過
するまでに細分化される。In the bioreactor of the first step, it is sufficient to cultivate the hardly decomposable substance-decomposing bacteria in the waste water, and as a result,
The enzyme that decomposes the hardly-decomposable substance is released to the outside of the cells, and the hardly-decomposable substance is decomposed into carbon dioxide gas and easily-decomposable substance by this decomposing enzyme. As a result, the polymer hardly decomposable substance is changed into a low molecular weight easily decomposable substance, and the substance is subdivided by the time it passes through the separation membrane in the next step.
【００３８】このバイオリアクターの名称は難分解性物
質分解菌を排水で培養する装置という意味で用いられ、
その意味では難分解性物質分解菌を浮遊状態に配置して
もよいし、担体に固定してもよい。微細な担体に固定し
て担体を排水中に浮遊させてもよく、担体をバイオリア
クター内に移動不能に配置してもよい。従って、活性汚
泥法や微生物膜法や固定化微生物法など種々のバイオリ
アクター方式が採用できる。The name of this bioreactor is used to mean a device for culturing persistently degrading bacteria in wastewater,
In that sense, the hardly decomposing substance-decomposing bacteria may be arranged in a floating state or may be fixed to a carrier. The carrier may be fixed to a fine carrier so that the carrier is suspended in the waste water, or the carrier may be immovably arranged in the bioreactor. Therefore, various bioreactor methods such as the activated sludge method, the microbial membrane method, and the immobilized microorganism method can be adopted.
【００３９】白色腐朽菌や糸状菌などの難分解性物質分
解菌は好気性菌であるから、菌体を活性化するために、
バイオリアクターには酸素を含む気体、主として空気を
供給し、バイオリアクター内の溶存酸素ＤＯは０．５ｍ
ｇ／Ｌ以上を維持することが望ましい。又、バイオリア
クター内の水温は１５〜３０℃、望ましくは２５〜３０
℃に維持する。この温度では難分解性物質分解菌の活性
が高くなり、効果的に分解酵素を産生して分解効率が高
くなる。Since the microorganisms decomposing persistent substances such as white rot fungi and filamentous fungi are aerobic bacteria, in order to activate the bacterial cells,
A gas containing oxygen, mainly air, is supplied to the bioreactor, and the dissolved oxygen DO in the bioreactor is 0.5 m.
It is desirable to maintain g / L or more. The water temperature in the bioreactor is 15 to 30 ° C, preferably 25 to 30 ° C.
Keep at ℃. At this temperature, the activity of the hardly decomposable substance-decomposing bacteria becomes high, and the decomposing enzyme is effectively produced, and the decomposition efficiency becomes high.
【００４０】第２工程では、バイオリアクターから排出
されるリアクター水を膜分離し、固体成分をバイオリア
クターに還流させ、液体成分と易分解性物質からなる排
水を選択的に後段工程に透過排出する。In the second step, the reactor water discharged from the bioreactor is membrane-separated, the solid component is refluxed to the bioreactor, and the waste water consisting of the liquid component and the easily decomposable substance is selectively permeated and discharged to the subsequent step. .
【００４１】バイオリアクター内では、難分解性物質分
解菌による分解処理の結果、水・易分解性物質・難分解
性物質・固形成分・微生物・菌体外酵素などが混合した
リアクター水として存在している。このリアクター水を
膜処理すると、水と低分子化した易分解性物質は膜を透
過して膜透過水を構成し、後段の工程へ送られる。膜と
しては、ＲＯ膜、ＵＦ膜、ＭＦ膜などが利用される。In the bioreactor, as a result of the decomposition treatment by the hardly decomposable substance-decomposing bacteria, water, easily decomposable substances, hardly decomposable substances, solid components, microorganisms, extracellular enzymes, etc. are present as reactor water. ing. When this reactor water is subjected to a membrane treatment, the water and the easily degradable substance having a low molecular weight permeate the membrane to form a membrane permeated water, which is sent to the subsequent step. An RO film, a UF film, an MF film, or the like is used as the film.
【００４２】しかし、高分子である難分解性物質・固形
成分・微生物（固定化微生物を含めて）・菌体外酵素な
どは膜を透過できず、バイオリアクターへとフィードバ
ックされる。この結果、バイオリアクター内の酵素濃度
は上昇し、次々と難分解性物質が分解され続ける。ま
た、フィードバックされた高分子の難分解性物質も再度
バイオリアクター内で処理され低分子化される。他方、
生成された易分解性物質は膜を透過して後段工程へと移
送される。However, macromolecules such as persistent substances, solid components, microorganisms (including immobilized microorganisms) and extracellular enzymes cannot pass through the membrane and are fed back to the bioreactor. As a result, the enzyme concentration in the bioreactor rises and the persistent substances are continuously decomposed. In addition, the fed-back polymer hardly decomposable substance is again processed in the bioreactor to be reduced in molecular weight. On the other hand,
The generated easily decomposable substance permeates the membrane and is transferred to the subsequent step.
【００４３】第３工程は膜透過水に含まれる易分解性物
質を分解処理する工程である。易分解性物質は、一般的
に低分子で水溶性でしかも通常の排水処理で分解除去す
ることが可能である。従って、この易分解性物質処理槽
では通常の排水処理技術が適用でき、例えば、凝集沈殿
槽や生物膜処理槽、活性汚泥処理槽などが適用できる。
生物膜処理法としては、例えば散水ろ床法、接触曝気
法、回転円板法など種々の方法が採用できる。従って、
この第３工程を経ることにより易分解性物質が除去さ
れ、排水は清澄な処理水となる。The third step is a step of decomposing the easily decomposable substance contained in the membrane-permeated water. Easily degradable substances are generally low-molecular, water-soluble, and can be decomposed and removed by ordinary wastewater treatment. Therefore, a normal wastewater treatment technique can be applied to this easily degradable substance treatment tank, for example, a coagulation sedimentation tank, a biofilm treatment tank, an activated sludge treatment tank, or the like.
As the biofilm treatment method, various methods such as a sprinkling filter method, a contact aeration method, and a rotating disk method can be adopted. Therefore,
By passing through the third step, the easily decomposable substance is removed and the wastewater becomes clear treated water.
【００４４】勿論、この第３工程から放出される処理水
に更に高度処理（三次処理）を施す事もできる。しか
し、第３工程を経た段階では既に高度に清澄化されてい
るから、一般の清澄処理水として環境にそのままの形態
で放出することも可能である。Of course, the treated water discharged from the third step can be subjected to a further advanced treatment (tertiary treatment). However, since it has already been highly clarified at the stage of passing through the third step, it is possible to release it as it is into the environment as general clarified treated water.
【００４５】また、第１工程で、雑菌の繁殖を防止する
ためにｐＨ調整剤を排水に投入して酸性化した場合に
は、第２工程の膜処理を経た膜透過水にアルカリ剤を投
入して中和処理する必要がある。そしてこの中和水を第
３工程の易分解性物質処理槽に供給する。In the first step, when a pH adjusting agent is added to the waste water to acidify it in order to prevent the growth of various bacteria, an alkaline agent is added to the membrane permeated water that has undergone the membrane treatment in the second step. Then, it is necessary to neutralize. Then, this neutralized water is supplied to the easily degradable substance treatment tank of the third step.
【００４６】図１は本発明に係る難分解性物質の分解処
理方法の実施形態の構成図である。排水ＯＷは、1次排
水である下水に対し砂沈殿処理・凝集沈殿処理・活性汚
泥処理を施した２次排水で、難分解性物質が残留してい
る。FIG. 1 is a block diagram of an embodiment of a method for decomposing a hardly decomposable substance according to the present invention. Wastewater OW is secondary wastewater obtained by subjecting sewage, which is the primary wastewater, to sand sedimentation treatment, coagulation sedimentation treatment, and activated sludge treatment, and persistent substances remain.
【００４７】排水ＯＷは、難分解性物質分解菌を浮遊状
態又は担体に固定した状態で配置したバイオリアクター
２に供給され、難分解性物質分解菌を培養するために必
要に応じて栄養源Ｎが添加されると同時に、雑菌の繁殖
を防止するために塩酸などのｐＨ調整剤Ｐが添加され
る。The wastewater OW is supplied to the bioreactor 2 in which the hardly-decomposing substance-decomposing bacteria are placed in a floating state or fixed on a carrier, and a nutrient source N is added as necessary for culturing the hardly-decomposing substance-decomposing bacteria. At the same time as is added, a pH adjuster P such as hydrochloric acid is added in order to prevent the growth of various bacteria.
【００４８】ブロアー４により空気Ａを曝気装置６に供
給し、排水ＯＷの中にミクロな空気泡を送って好気性の
難分解性分解菌を活性化させる。バイオリアクター２の
中で難分解性物質は炭酸ガス及び易分解性物質へと分解
され、排水ＯＷはリアクター水ＭＷへと変化する。Air A is supplied to the aerator 6 by the blower 4 and micro air bubbles are sent into the waste water OW to activate the aerobic persistent decomposing bacteria. In the bioreactor 2, the hardly decomposable substance is decomposed into carbon dioxide gas and easily decomposable substance, and the wastewater OW is changed into the reactor water MW.
【００４９】ポンプ８によりリアクター水ＭＷを膜処理
装置９に供給する。リアクター水ＭＷの中の固形成分及
び高分子成分ＦＳは膜を透過できず、この固形成分及び
高分子成分ＦＳはバイオリアクター２にフィードバック
される。固形成分及び高分子成分ＦＳは、難分解性物
質、微生物（固定化微生物を含む）、菌体外酵素などか
らなる。The reactor water MW is supplied to the membrane treatment device 9 by the pump 8. The solid component and the polymer component FS in the reactor water MW cannot pass through the membrane, and the solid component and the polymer component FS are fed back to the bioreactor 2. The solid component and the polymer component FS are composed of a persistent substance, a microorganism (including immobilized microorganisms), an extracellular enzyme, and the like.
【００５０】リアクター水ＭＷの中で、水と易分解性物
質は膜の微小孔を透過し、膜透過水ＩＷとなる。この膜
透過水ＩＷは中和処理槽１０に送られ、ここでアルカリ
性物質が添加されて中和処理が行なわれる。この中和水
ＮＷが次の易分解物質処理槽１２に供給される。In the reactor water MW, water and the easily decomposable substance permeate through the micropores of the membrane to become the membrane permeated water IW. The membrane permeated water IW is sent to the neutralization treatment tank 10, where an alkaline substance is added and the neutralization treatment is performed. This neutralized water NW is supplied to the next easily degradable substance treatment tank 12.
【００５１】易分解性物質処理槽１２では通常の排水処
理である活性汚泥による生物処理を行なってもよい。通
常の活性汚泥法を用いた場合には、汚泥を分離するため
の沈殿池などの設備が必要となる。他方、この実施形態
では生物膜処理槽が採用されている。この易分解性物質
処理槽１２により中和水ＮＷに含まれる易分解性物質が
除去され、極めて清澄な処理水ＴＷが環境へと放出され
る。この処理水の含有物質濃度を更に低減する必要があ
る場合には、別の高度処理が選択的に実施される。In the easily degradable substance treatment tank 12, biological treatment using activated sludge, which is a normal wastewater treatment, may be performed. When the ordinary activated sludge method is used, equipment such as a sedimentation tank for separating the sludge is required. On the other hand, in this embodiment, a biofilm treatment tank is adopted. The easily degradable substance treatment tank 12 removes the easily degradable substance contained in the neutralized water NW, and releases the extremely clear treated water TW to the environment. When it is necessary to further reduce the concentration of substances contained in the treated water, another advanced treatment is selectively carried out.
【００５２】[0052]
【実施例】[実施例１：ダイオキシン類の分解]難分解性
物質分解菌として白色腐朽菌のトゥラメテス・ヒルスタ
（Trametes hirsuta）を用いた。この菌株は財団法人発
酵研究所（ＩＦＯ）から入手できるＩＦＯ４９１７株で
ある。[Examples] [Example 1: Decomposition of dioxins] White rot fungus Trametes hirsuta was used as a microorganism decomposing a hardly decomposable substance. This strain is the IFO4917 strain available from the Fermentation Research Institute (IFO).
【００５３】このＩＦＯ４９１７株をまずＧＰＹ培地に
接種し、２５℃で４日間培養した。この菌体を分離液で
更に７日間培養して馴致した。この馴致した菌体により
バイオリアクターを構成し、実際の排水を用いて分解処
理を行なった。排水、リアクター水、膜透過水の中の毒
性当量を測定した。結果を表１に示す。毒性当量の単位
はpg-TEQ/Lである。The IFO4917 strain was first inoculated into GPY medium and cultured at 25 ° C. for 4 days. The cells were cultivated with the separated liquid for another 7 days for acclimatization. A bioreactor was constructed from the acclimatized cells and the degradation process was performed using actual wastewater. Toxic equivalents in wastewater, reactor water and membrane permeate were measured. The results are shown in Table 1. The unit of toxic equivalent is pg-TEQ / L.
【００５４】 ＜表１＞ダイオキシン類分解試験 ＜排水＞ ＜リアクター水＞ ＜膜透過水＞ ＜毒性当量＞ ＜毒性当量＞＜除去率＞ ＜毒性当量＞ ＜除去率＞ 1.2pg-TEQ/L 0.051pg-TEQ/L 96% 0.0055pg-TEQ/L >99%[0054] <Table 1> Dioxin decomposition test <Drainage> <Reactor water> <Membrane permeate> <Toxic equivalent> <Toxic equivalent> <Removal rate> <Toxic equivalent> <Removal rate> 1.2pg-TEQ / L 0.051pg-TEQ / L 96% 0.0055pg-TEQ / L> 99%
【００５５】ダイオキシン類について排水中の環境基準
値は１pg-TEQ/L以下であるから、排水中の毒性当量は基
準値をやや超えている。しかし、リアクター処理水（リ
アクター水と表記）では排水中のダイオキシン類は９６
％が除去され、膜透過水では９９％以上が除去されてい
る。この発明処理によって排水中濃度の１／１００以下
にダイオキシン類の濃度を低減することに成功した。同
時に、膜透過水の毒性当量は、環境基準値を大幅にクリ
ア−することが実証できた。Regarding dioxin, since the environmental standard value in wastewater is 1 pg-TEQ / L or less, the toxicity equivalent in the wastewater slightly exceeds the standard value. However, in the reactor treated water (denoted as reactor water), dioxins in the wastewater are 96
%, And 99% or more of the membrane-permeated water is removed. The treatment according to the present invention succeeded in reducing the concentration of dioxins to 1/100 or less of the concentration in wastewater. At the same time, it could be demonstrated that the toxic equivalent of the permeated water of the membrane significantly cleared the environmental standard value.
【００５６】[実施例２：環境ホルモンの分解]実施例１
と同様に、難分解性物質分解菌として白色腐朽菌のトゥ
ラメテス・ヒルスタ（Trametes hirsuta）のIＦＯ４９
１７株を用いた。この菌株を前述と同様の条件で培養
し、担体に固定してバイオリアクター内に配置した。[Example 2: Decomposition of environmental hormones] Example 1
Similarly, IFO49 of Trametes hirsuta which is a white-rot fungus that decomposes persistent substances
17 strains were used. This strain was cultured under the same conditions as described above, fixed on a carrier and placed in a bioreactor.
【００５７】実際の排水を用いて、環境ホルモン分解実
験を行なった。排水には環境ホルモンとして、ノニルフ
ェノール、4-t-オクチルフェノール、ベンゾフェノンの
３種類が含まれていた。排水、リアクター水、膜透過水
の中の濃度を測定した。結果を表２に示す。環境基準値
は定められていないので、濃度の単位はμｇ/Lが用いら
れた。An environmental hormone decomposition experiment was carried out using actual waste water. The wastewater contained three types of environmental hormones, nonylphenol, 4-t-octylphenol, and benzophenone. The concentrations in wastewater, reactor water and membrane permeate were measured. The results are shown in Table 2. Since the environmental standard value is not defined, the unit of concentration was μg / L.
【００５８】 ＜表１＞環境ホルモン分解試験 ＜環境ホルモン＞ ＜排水＞ ＜リアクター水＞ ＜膜透過水＞ ＜濃度＞ ＜濃度＞ ＜除去率＞ ＜濃度＞ ＜除去率＞ノニルフェノール 1.6μｇ/L 0.3μｇ/L 81% <0.1μｇ/L >94%* 4-t-オクチルフェノール 2.9 0.7 76 0.32 89ヘ゛ンソ゛フェノン 0.13 0.04 69 <0.025 >81* （測定方法は固相抽出―ＧＣ−ＭＳ法、＊印は定量下限値未満まで除去）<Table 1> Environmental hormone decomposition test <Environmental hormone><Drainage><Reactorwater><Membranepermeate><Concentration><Concentration><Removalrate><Concentration><Removalrate> Nonylphenol 1.6 μg / L 0.3 μg / L 81% <0.1 μg / L> 94% * 4-t-octylphenol 2.9 0.7 76 0.32 89 Benzophenone 0.13 0.04 69 <0.025> 81 * (Measurement method is solid phase extraction-GC-MS method, * mark is quantitative Removed below the lower limit)
【００５９】環境ホルモンに関しては、現段階では環境
基準値がまだ法定されていないので、出来るだけ濃度を
低下させることが望ましいという観点で判断できる。こ
の実施例では、３種類の環境ホルモンに対してこの発明
処理を行なうと、膜透過水の濃度を排水濃度の約１／１
０にまで低下できることが分かった。従って、本発明は
環境ホルモンに対しても有効であることが分かる。Regarding the endocrine disrupters, since the environmental standard values have not yet been statutory at this stage, it can be judged from the viewpoint that it is desirable to reduce the concentration as much as possible. In this example, when the treatment of the present invention was applied to three kinds of environmental hormones, the concentration of the membrane permeated water was reduced to about 1/1 of the wastewater concentration.
It turned out that it can be reduced to zero. Therefore, it can be seen that the present invention is also effective against endocrine disrupters.
【００６０】本発明は上記実施形態及び実施例に限定さ
れるものではなく、本発明の技術的思想を逸脱しない範
囲における種々の変形例及び設計変更をその技術的範囲
内に包含するものであることは言うまでもない。The present invention is not limited to the above-described embodiments and examples, but includes various modifications and design changes within the technical scope of the present invention without departing from the technical idea of the present invention. Needless to say.
【００６１】[0061]
【発明の効果】請求項１の発明によれば、難分解性物質
分解菌を利用して排水中の難分解性物質を分解し、この
分解によって生成された易分解性物質を易分解性物質処
理槽により除去できるから、微生物処理により安価かつ
省エネルギーな排水処理を実現し、大幅なエネルギー削
減を可能にする。According to the first aspect of the present invention, the hardly decomposable substance in the waste water is decomposed by utilizing the hardly decomposable substance-decomposing bacterium, and the easily decomposable substance produced by this decomposition is easily decomposed. Since it can be removed by the treatment tank, the microbial treatment realizes an inexpensive and energy-saving wastewater treatment and enables a significant energy reduction.
【００６２】請求項２の発明によれば、難分解性物質分
解菌として白色腐朽菌又は糸状菌を利用するから、これ
らの微生物が産生する酵素により難分解性物質を分解で
き、排水処理におけるランニングコストの低減化を実現
できる。According to the second aspect of the present invention, since white rot fungi or filamentous fungi are used as the microorganisms decomposing the hardly decomposable substance, it is possible to decompose the hardly decomposable substance by the enzymes produced by these microorganisms, and the running in the wastewater treatment is performed. Cost reduction can be realized.
【００６３】請求項３の発明によれば、排水中のダイオ
キシン類や外因性内分泌撹乱物質（環境ホルモン）を有
効に分解処理できる。According to the third aspect of the present invention, dioxins and exogenous endocrine disrupting substances (environmental hormones) in wastewater can be effectively decomposed.
【００６４】請求項４の発明によれば、難分解性物質分
解菌を担体に固定した微生物固定担体をバイオリアクタ
ーに配置するから、固体触媒と同様に微生物を生体触媒
として繰り返し安全に使用することができる。According to the fourth aspect of the present invention, since the microorganism-immobilized carrier in which the hardly decomposable substance-decomposing bacterium is immobilized on the carrier is arranged in the bioreactor, the microorganism can be repeatedly and safely used as a biocatalyst like the solid catalyst. You can
【００６５】請求項５の発明によれば、易分解性物質処
理槽を生物膜処理槽で構成するから、バイオリアクター
との組み合わせにより難分解性物質を一貫して生物処理
により分解処理することができる。According to the invention of claim 5, since the easily degradable substance treatment tank is constituted by a biofilm treatment tank, it is possible to consistently decompose the hardly decomposable substance by biological treatment in combination with the bioreactor. it can.
【００６６】このように、本発明では白色腐朽菌や糸状
菌などの難分解性物質分解菌を用いるから、従来の生物
処理法では得られなかった排水中の難分解性物質の分解
除去を達成したものである。従って、物理化学的処理に
頼っていた従来技術と比較すると、極めて安価にしかも
大幅なエネルギー削減効果を有する排水の処理方法を提
供できる。As described above, in the present invention, since the microorganisms decomposing hardly decomposable substances such as white rot fungi and filamentous fungi are used, the decomposition and removal of the hardly decomposable substances in the wastewater, which cannot be obtained by the conventional biological treatment method, are achieved. It was done. Therefore, it is possible to provide a method for treating wastewater that is extremely inexpensive and has a large energy reduction effect, as compared with the conventional technique that relies on physicochemical treatment.
【図１】本発明に係る難分解性物質の分解処理方法の実
施形態の構成図である。FIG. 1 is a configuration diagram of an embodiment of a decomposition treatment method for a hardly decomposable substance according to the present invention.
２はバイオリアクター、４はブロワ、６は曝気装置、８
はポンプ、９は膜処理装置、１０は中和処理槽、１２は
易分解性物質処理槽、Ａは空気、ＦＳは固形成分、ＩＷ
は膜透過水、ＭＷはリアクター水（リアクター処理
水）、ＮＷは中和水、ＯＷは排水、ＰはｐＨ調整剤、Ｔ
Ｗは処理水である。2 is a bioreactor, 4 is a blower, 6 is an aerator, 8
Is a pump, 9 is a membrane treatment device, 10 is a neutralization treatment tank, 12 is an easily decomposable substance treatment tank, A is air, FS is a solid component, IW
Is membrane permeated water, MW is reactor water (reactor-treated water), NW is neutralized water, OW is wastewater, P is pH adjuster, T
W is treated water.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） //(Ｃ１２Ｎ 1/00 Ｃ１２Ｒ 1:645 Ｃ１２Ｒ 1:645） (72)発明者 福里 豊 兵庫県尼崎市金楽寺町２丁目２番33号 株 式会社タクマ内 Ｆターム(参考） 4B065 AA57X AC20 BA22 BB06 BB07 BB40 CA56 4D003 AA14 AB02 BA02 BA03 CA02 DA09 FA06 4D006 GA03 GA06 GA07 KA02 KB22 PA01 PB08 PC67 4D028 AB00 AC06 BB02 BC17 BD11 BD17 CA07 CB02 ─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. 7 Identification code FI theme code (reference) // (C12N 1/00 C12R 1: 645 C12R 1: 645) (72) Inventor Yutaka Fukusato Amagasaki City, Hyogo Prefecture 2 2-33 Kinrakuji-cho F-Term in Takuma Co., Ltd. (Reference) 4B065 AA57X AC20 BA22 BB06 BB07 BB40 CA56 4D003 AA14 AB02 BA02 BA03 CA02 DA09 FA06 4D006 GA03 GA06 GA07 KA02 KB22 PA01 PB08 PC67 4D028 AB00 AC11 BD17 BC02 CA07 CB02
Claims (5)
物質を分解する菌株により排水に含有される難分解性物
質を炭酸ガス及び易分解性物質にまで分解する工程と、
前記バイオリアクターから排出されるリアクター水を膜
分離して高分子成分及び固体成分をバイオリアクターに
還流させ液体成分と易分解性物質からなる排水を透過排
出する工程と、この膜透過水を易分解性物質処理槽に供
給して易分解性物質を分解処理し清澄な処理水を放出す
ることを特徴とする排水中の難分解性物質の分解処理方
法。1. A step of decomposing a hardly decomposable substance contained in wastewater into carbon dioxide gas and a readily decomposable substance by a strain that decomposes the hardly decomposable substance arranged in a bioreactor,
Reactor water discharged from the bioreactor is membrane-separated and polymer components and solid components are refluxed to the bioreactor to permeate and discharge wastewater composed of liquid components and easily decomposable substances, and the membrane permeated water is easily decomposed. A method for decomposing a hardly decomposable substance in wastewater, which comprises supplying to a treatment tank for degradable substances to decompose easily decomposable substances and releasing clear treated water.
腐朽菌又は糸状菌である請求項１に記載の排水中の難分
解性物質の分解処理方法。2. The method for decomposing a persistent substance in wastewater according to claim 1, wherein the strain decomposing the persistent substance is a white rot fungus or a filamentous fungus.
外因性内分泌撹乱物質である請求項１又は２に記載の排
水中の難分解性物質の分解処理方法。3. The method for decomposing a hardly decomposable substance in wastewater according to claim 1, wherein the hardly decomposable substance is a dioxin or an exogenous endocrine disrupting substance.
定した微生物固定担体を前記バイオリアクターに配置す
る請求項１に記載の排水中の難分解性物質の分解処理方
法。4. The method for decomposing a hardly decomposable substance in wastewater according to claim 1, wherein a microorganism-immobilized carrier having a strain that decomposes the hardly decomposable substance immobilized on a carrier is arranged in the bioreactor.
である請求項１に記載の排水中の難分解性物質の分解処
理方法。5. The method for decomposing a hardly decomposable substance in wastewater according to claim 1, wherein the easily decomposable substance treatment tank is a biofilm treatment tank.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006281042A (en) * | 2005-03-31 | 2006-10-19 | Suminoe Textile Co Ltd | Continuous porous molded body immobilizing microbe and method for discoloring dye using this |
| CN102259986A (en) * | 2011-07-21 | 2011-11-30 | 绍兴文理学院 | Method for rapidly reducing chromaticity of printing and dying wastewater by utilizing white rot fungi |
-
2001
- 2001-10-24 JP JP2001326123A patent/JP2003126883A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006281042A (en) * | 2005-03-31 | 2006-10-19 | Suminoe Textile Co Ltd | Continuous porous molded body immobilizing microbe and method for discoloring dye using this |
| CN102259986A (en) * | 2011-07-21 | 2011-11-30 | 绍兴文理学院 | Method for rapidly reducing chromaticity of printing and dying wastewater by utilizing white rot fungi |
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