JP2003093038A5 - - Google Patents

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JP2003093038A5
JP2003093038A5 JP2001289938A JP2001289938A JP2003093038A5 JP 2003093038 A5 JP2003093038 A5 JP 2003093038A5 JP 2001289938 A JP2001289938 A JP 2001289938A JP 2001289938 A JP2001289938 A JP 2001289938A JP 2003093038 A5 JP2003093038 A5 JP 2003093038A5
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Japan
Prior art keywords
nucleic acid
unit
container
reaction vessel
reagent
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JP2001289938A
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Japanese (ja)
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JP2003093038A (en
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Priority to JP2001289938A priority Critical patent/JP2003093038A/en
Priority claimed from JP2001289938A external-priority patent/JP2003093038A/en
Priority to US10/246,959 priority patent/US20030059823A1/en
Publication of JP2003093038A publication Critical patent/JP2003093038A/en
Publication of JP2003093038A5 publication Critical patent/JP2003093038A5/ja
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Claims (9)

少なくとも（Ａ）反応容器保持具及び加温・冷却装置を備えてなるディネーチャー部、（Ｂ）反応容器保持具及び加温・冷却装置を備えてなるアニーリング部、（Ｃ）反応容器保持具及び磁力制御装置を備えてなる磁気分離部、（Ｄ）チップラックを備えてなるチップラック収納部、（Ｅ）洗浄液収容容器を備えてなる洗浄液部、（Ｆ）廃液収容容器を備えてなる廃液部及び（Ｇ）複数本のチップノズルを装備し、該チップノズルにチップを装着・脱着させる機構と、装着されたチップが処理液を吸引・注入する機構と、反応容器の把持及び離脱を自由に行わせることが可能なロボットハンド機構とを有し、Ｘ−Ｚ軸方向へ自在に移動可能なアームユニットを備えてなるヘッド部、より構成される自動核酸ハイブリダイゼーション装置。  At least (A) a denaturer unit comprising a reaction vessel holder and a heating / cooling device, (B) an annealing unit comprising a reaction vessel holder and a heating / cooling device, (C) a reaction vessel holder and Magnetic separation unit including a magnetic force control device, (D) Chip rack storage unit including a chip rack, (E) Cleaning liquid unit including a cleaning liquid storage container, (F) Waste liquid unit including a waste liquid storage container And (G) Equipped with a plurality of tip nozzles, a mechanism for attaching / detaching the tip to / from the tip nozzle, a mechanism for the attached tip to suck / inject the processing liquid, and a reaction vessel to be freely grasped and detached An automatic nucleic acid hybridization device comprising a head unit having a robot hand mechanism that can be performed and having an arm unit that can move freely in the XZ axis direction. （Ｆ）廃液部を（Ｄ）チップラック収納部の下部に配置する請求項１記載の自動核酸ハイブリダイゼーション装置。  The automatic nucleic acid hybridization apparatus according to claim 1, wherein (F) the waste liquid part is disposed below the (D) chip rack storage part. 更に、（Ｈ）試薬収容容器を備えてなる試薬部をもつ請求項１又は２記載の自動核酸ハイブリダイゼーション装置。  The automatic nucleic acid hybridization device according to claim 1 or 2, further comprising (H) a reagent part comprising a reagent container. 下記工程（１）〜（１０）、所望により更に工程（１１）〜（１５）を自動で行い、次いで反応容器中の標識核酸量を測定することを特徴とするサンプル中の核酸検出方法。
（１）核酸プローブ固定化磁性粒子、標識プローブ及びサンプル核酸、又は核酸プローブ固定化磁性粒子及び標識されたサンプル核酸が注入・混合された反応容器をディネーチャー部に設置し、加温・冷却装置により反応容器中の温度を核酸のディネーチャー温度に設定し、当該温度を所定時間保持してサンプル核酸を一本鎖化する。
（２）アームユニットを稼働してディネーチャー部の反応容器をアニーリング部に移送する。
（３）アニーリング部の加温・冷却装置により反応容器中の温度を核酸のアニーリング温度に設定し、当該温度を所定時間保持してアニーリングを行なう。
（４）アームユニットをアニーリング部に移動し、反応容器を磁気分離部に移送する。
（５）磁力制御装置を稼働して磁性粒子に結合したサンプル核酸を容器中に偏在させる。
（６）アームユニットをチップラック収納部に移動し、チップノズルにチップを装着する。
（７）アームユニットを磁気分離部に移動し、反応容器中の上清をチップノズルにて吸引する。
（８）アームユニットを廃液部に移動し、チップノズル内の吸引上清を廃液部の廃棄収容容器に排出する。
（９）アームユニットを洗浄液部に移動し、洗浄液収容容器から洗浄液を吸引し、磁気分離部の反応容器中に洗浄液を注入する。
（１０）（７）〜（９）の洗浄動作を所定回数繰り返す。
（１１）（７）〜（８）を行った後、アームユニットを第一試薬部へ移動させ、試薬収容容器から標識試薬を吸引し、磁気分離部の反応容器中にこれを注入し、所定時間保持する。
（１２）反応容器中の上清をチップノズルにて吸引し、アームユニットを廃液部に移動し、チップノズル内の吸引上清を廃液部の廃棄収容容器に排出する。
（１３）アームユニットを第二洗浄液部へ移動し、第二洗浄液収容容器より洗浄液を吸引し、磁気分離部の反応容器中にこれを注入し、所定時間保持する。
（１４）（１２）〜（１３）の洗浄動作を所定回数繰り返した後（１２）の動作を行う。
（１５）アームユニットを第二試薬部へ移動し、試薬収容容器から発色試薬を吸引し、磁気分離部の反応容器中にこれを注入する。A method for detecting a nucleic acid in a sample, comprising automatically performing the following steps (1) to (10), and further, if necessary, further steps (11) to (15) and then measuring the amount of labeled nucleic acid in a reaction vessel.
(1) A nucleic acid probe-immobilized magnetic particle, a labeled probe and a sample nucleic acid, or a reaction vessel in which a nucleic acid probe-immobilized magnetic particle and a labeled sample nucleic acid are injected and mixed is installed in a deinator unit, and a heating / cooling device Thus, the temperature in the reaction vessel is set to the nucleic acid denaturer temperature, and the sample nucleic acid is made into a single strand by maintaining the temperature for a predetermined time.
(2) The arm unit is operated to transfer the reaction vessel of the deny part to the annealing part.
(3) The temperature in the reaction vessel is set to the annealing temperature of the nucleic acid by the heating / cooling device of the annealing section, and annealing is performed while maintaining the temperature for a predetermined time.
(4) The arm unit is moved to the annealing unit, and the reaction vessel is transferred to the magnetic separation unit.
(5) Operate the magnetic force control device to make the sample nucleic acid bound to the magnetic particles unevenly distributed in the container.
(6) The arm unit is moved to the chip rack storage unit, and the chip is mounted on the chip nozzle.
(7) The arm unit is moved to the magnetic separation unit, and the supernatant in the reaction vessel is sucked with the tip nozzle.
(8) The arm unit is moved to the waste liquid part, and the suction supernatant in the tip nozzle is discharged to the waste container of the waste liquid part.
(9) Move the arm unit to the cleaning liquid section, suck the cleaning liquid from the cleaning liquid storage container, and inject the cleaning liquid into the reaction container of the magnetic separation section.
(10) The cleaning operations (7) to (9) are repeated a predetermined number of times.
(11) After performing (7) to (8), the arm unit is moved to the first reagent section, the labeling reagent is sucked from the reagent storage container, and this is injected into the reaction container of the magnetic separation section. Hold for hours.
(12) The supernatant in the reaction container is sucked with the tip nozzle, the arm unit is moved to the waste liquid part, and the suction supernatant in the chip nozzle is discharged into the waste container of the waste liquid part.
(13) The arm unit is moved to the second cleaning liquid section, the cleaning liquid is sucked from the second cleaning liquid storage container, poured into the reaction container of the magnetic separation section, and held for a predetermined time.
(14) After the cleaning operations (12) to (13) are repeated a predetermined number of times, the operation (12) is performed.
(15) The arm unit is moved to the second reagent unit, the coloring reagent is sucked from the reagent storage container, and this is injected into the reaction container of the magnetic separation unit. 反応容器として、核酸プローブ固定化磁性粒子を予め備えた試薬ユニットを使用する請求項４記載の核酸検出方法。  The nucleic acid detection method according to claim 4, wherein a reagent unit provided in advance with nucleic acid probe-immobilized magnetic particles is used as the reaction container. 反応容器として、核酸プローブ固定化磁性粒子と発光標識用プローブとを予め備えた試薬ユニットを使用する請求項４記載の核酸検出方法。  The nucleic acid detection method according to claim 4, wherein a reagent unit previously provided with nucleic acid probe-immobilized magnetic particles and a luminescent labeling probe is used as the reaction container. 磁性粒子が、磁性細菌粒子である請求項４〜６記載の核酸検出方法。  The nucleic acid detection method according to claim 4, wherein the magnetic particles are magnetic bacterial particles. 磁性粒子に固定化された核酸が、一本鎖ＤＮＡ、ＲＮＡ又はＰＮＡである請求項４〜７のいずれか１項記載の核酸検出方法。  The nucleic acid detection method according to any one of claims 4 to 7, wherein the nucleic acid immobilized on the magnetic particles is single-stranded DNA, RNA, or PNA. サンプル核酸が、蛍光色素、アルカリフォスファターゼ又はフェロセンによりマーカー標識されたものである請求項４〜８のいずれか１項記載の核酸検出方法。  The nucleic acid detection method according to any one of claims 4 to 8, wherein the sample nucleic acid is labeled with a fluorescent dye, alkaline phosphatase or ferrocene.
JP2001289938A 2001-09-21 2001-09-21 Hybridization apparatus and method for detecting nucleic acid in sample by using the same Pending JP2003093038A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2001289938A JP2003093038A (en) 2001-09-21 2001-09-21 Hybridization apparatus and method for detecting nucleic acid in sample by using the same
US10/246,959 US20030059823A1 (en) 2001-09-21 2002-09-18 Hybridization apparatus and method for detecting nucleic acid in sample using the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2001289938A JP2003093038A (en) 2001-09-21 2001-09-21 Hybridization apparatus and method for detecting nucleic acid in sample by using the same

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JP2003093038A JP2003093038A (en) 2003-04-02
JP2003093038A5 true JP2003093038A5 (en) 2005-08-25

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Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005008209A2 (en) * 2003-07-16 2005-01-27 Toyo Boseki Device for separating biological component and method of separating biological component with the use thereof
SG125967A1 (en) * 2004-07-09 2006-10-30 Nanyang Polytechnic Hybridization apparatus
EP2192186B1 (en) * 2008-11-28 2016-03-09 F. Hoffmann-La Roche AG System and method for the automated extraction of nucleic acids
JP5933918B2 (en) * 2009-12-10 2016-06-15 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Mold-shaped locking system
JP5689722B2 (en) * 2010-04-01 2015-03-25 株式会社東芝 Automatic analyzer
KR102001137B1 (en) * 2017-07-28 2019-07-17 (주)옵토레인 Sample preparation device, method of manufacturing the same, and method of preparing sample using the same
JP6680742B2 (en) * 2017-10-31 2020-04-15 大研医器株式会社 Magnetic particle collection method and test set
CN111849750B (en) * 2020-06-23 2022-10-28 福建工程学院 Automatic detection system of genotyping chip

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