JP2003061643A - Oil-treating material containing microorganism and method for treating oil by using the same - Google Patents
Oil-treating material containing microorganism and method for treating oil by using the sameInfo
- Publication number
- JP2003061643A JP2003061643A JP2001252063A JP2001252063A JP2003061643A JP 2003061643 A JP2003061643 A JP 2003061643A JP 2001252063 A JP2001252063 A JP 2001252063A JP 2001252063 A JP2001252063 A JP 2001252063A JP 2003061643 A JP2003061643 A JP 2003061643A
- Authority
- JP
- Japan
- Prior art keywords
- oil
- treating
- microorganism
- microorganisms
- treatment material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、工場やレストラン
及び家庭からでる含油排水、水域への油流出事故、操業
及び事故等による陸上への油流出、を安全に処理し、吸
収した油分を処理材の上で微生物により分解・除去する
ための微生物含有油処理材およびそれを用いた油の処理
方法に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention safely treats oil-containing wastewater discharged from factories, restaurants and homes, oil spill accidents to water areas, oil spill to land due to operations and accidents, and processes absorbed oil. TECHNICAL FIELD The present invention relates to a microorganism-containing oil treatment material for decomposing and removing microorganisms on a material and an oil treatment method using the same.
【０００２】[0002]
【従来の技術】現在行なわれている水域及び陸上で流出
した油の処理方法は、吸着材に吸着させる、バケツやヒ
シャクで掬う、バキュームカーや油分回収船で吸収する
などの方法を含めた回収方法等が行なわれている。回収
した油分は廃棄物として燃焼や埋立て処理されている。2. Description of the Related Art Currently used methods for treating oil spilled in waters and onshore include recovery methods such as adsorbing it on an adsorbent, scooping it with a bucket or Hisaku, and absorbing it with a vacuum car or oil recovery ship. The method etc. are carried out. The recovered oil is burned or landfilled as waste.
【０００３】従来の方法では、水域での油分回収に泥
炭、籾殻、活性炭、木、オガクズ、木の実の殻、紙、植
物繊維、綿、布、セルロース、スポンジ、海綿、毛、ガ
ラス繊維、発泡スチロール、セラミック、ポリプロピレ
ン、ウレタンフォームその他多孔質状、又は、毛細管作
用のある物等が油吸収材として使用されていた。これら
の多くのものは一時的に水に浮くものの、徐々に吸水し
て水面下に沈んでしまう欠点があった。さらに、油を吸
収させた後はその処理方法としては焼却処分や埋立て処
分が適用されている。このような処理方法は環境には必
ずしも迎合する処分方法ではない。In the conventional method, peat, rice husk, activated carbon, wood, sawdust, nut shell, paper, vegetable fiber, cotton, cloth, cellulose, sponge, sponge, wool, glass fiber, Styrofoam, are used for recovering oil in water. Ceramics, polypropylene, urethane foam, other porous materials, or materials having a capillary action have been used as oil absorbents. Although many of these float on the water temporarily, they have the drawback that they gradually absorb water and sink below the surface of the water. Furthermore, after absorbing the oil, incineration or landfill is applied as the treatment method. Such treatment methods are not necessarily environmentally compliant disposal methods.
【０００４】[0004]
【発明が解決しようとする課題】本発明の目的は、この
欠点を除くために水に浮遊して迅速に油分を吸収する素
材を用い、吸収材上で吸収した油分を微生物により分解
除去するための微生物含有油処理材およびそれを用いた
油の処理方法を提供する点にある。The object of the present invention is to use a material that floats in water and quickly absorbs oil in order to eliminate this drawback, and to decompose and remove the oil absorbed by the absorbent by microorganisms. Another object of the present invention is to provide an oil treatment material containing a microorganism and an oil treatment method using the same.
【０００５】[0005]
【課題を解決するための手段】本発明の特徴の第１は、
多孔質の嵩密度、すなわち充填密度（日本工業規格ＪＩ
Ｓ Ｋ１４７４、１７ページ）の比較的小さい炭、すな
わち嵩密度０．８以下、好ましくは０．７以下、とくに
好ましくは０．５以下の炭を吸収材として用いる点にあ
る。嵩密度が０．８より大きいものは、実質上粒子の最
上部がやっと水面上にある状態であり、波あるいは撹拌
にさらされると粒空隙の１部に水が侵入し、炭粒子が水
中に沈降する結果になるので好ましくない。この面の安
全性を考えると０．７以下、とくに０．５以下であるこ
とが好ましい。嵩密度の下限は、格別の制約はないが、
おおむね０．０５であり、これ以下になると多孔質粒子
としての取扱いが難しくなるので、好ましくない。油を
吸収する素材は、素材が親油性であるもの、多孔質であ
るもの、更には両方を兼ね備えているものが挙げられ
る。親油性素材は発泡ポリスチロール、発泡ポリプロピ
レン、ウレタンフォーム、泥炭（集積した分解不完全な
植物遺体の堆積物で炭ではない）、木の実の殻、毛等多
くのものが挙げられる。一方、多孔質素材は籾殻、オガ
クズ、紙、植物繊維、綿、布、セルロース、スポンジ、
海綿、ガラス繊維等多くの物が挙げられる。しかしなが
らこれらの物はその性質上、水に浮遊した油を処理する
場合には素材そのものが水を吸って沈んでしまったり、
油吸収量が素材表面に限られるため吸収量が少なかった
り、回収時に吸収材から他の接触している道具や物体へ
油が付着して汚染を拡散することが多かった。その点親
油性で多孔質を兼ね備えた素材はこれら問題を解決する
かに見えるが、素材の種類によっては水に浮遊せず水面
下に沈降してしまうものがある。炭はその性質上親油性
であるが、炭素の比重はその構造上一番軽い無定形炭素
であっても１．８〜２．１であり水より重い。しかしな
がら、炭の製法条件により本発明に適した見かけ上の比
重の軽い、即ち嵩密度（充填密度）の小さい多孔質の炭
を作る事が出来る。この例を下の表に示す。The first feature of the present invention is as follows.
Porous bulk density, that is, packing density (Japanese Industrial Standard JI
SK 1474, p. 17), that is, a comparatively small charcoal, that is, a charcoal having a bulk density of 0.8 or less, preferably 0.7 or less, particularly preferably 0.5 or less is used as an absorbent. When the bulk density is more than 0.8, the uppermost part of the particles is substantially on the water surface, and when exposed to waves or agitation, water penetrates into a part of the particle voids, causing the carbon particles to enter the water. It is not preferable because it will result in sedimentation. Considering the safety in this aspect, it is preferably 0.7 or less, particularly 0.5 or less. The lower limit of bulk density is not particularly limited,
It is about 0.05, and if it is less than 0.05, it becomes difficult to handle as porous particles, which is not preferable. Examples of materials that absorb oil include those that are lipophilic, those that are porous, and those that have both. Examples of lipophilic materials include expanded polystyrene, expanded polypropylene, urethane foam, peat (accumulation of incompletely decomposed plant remains, not charcoal), nut shells, hair and many others. On the other hand, porous materials include chaff, sawdust, paper, vegetable fiber, cotton, cloth, cellulose, sponge,
Many examples include sponges and glass fibers. However, due to the nature of these things, when processing oil floating in water, the material itself absorbs water and sinks,
Since the amount of oil absorbed was limited to the surface of the material, the amount of oil absorbed was small, and during collection, the oil often adhered to other tools or objects that were in contact with it to diffuse the pollution. In that respect, materials that are both lipophilic and porous appear to solve these problems, but depending on the type of material, some materials do not float in water and settle below the water surface. Charcoal is lipophilic in nature, but the specific gravity of carbon is 1.8 to 2.1, which is heavier than water, even if it is the lightest amorphous carbon in its structure. However, porous charcoal having an apparently low specific gravity suitable for the present invention, that is, a porous charcoal having a small bulk density (packing density), can be produced depending on the charcoal production conditions. An example of this is shown in the table below.
【０００６】[0006]
【表１】 [Table 1]
【０００７】これらの炭は、通常０．５ｍｍ〜１０ｍｍ
程度の粒子、とくに細長い米粒状の粒子として使用す
る。These charcoals are usually 0.5 mm to 10 mm
It is used as a medium-sized particle, especially as an elongated rice-shaped particle.
【０００８】本発明の特徴の第２は、油吸収炭すなわち
嵩密度０．８以下の炭粒子に油分解能を有する微生物
（微生物製剤も含む）を共存させる点にある。微生物の
炭粒子に対する担持量は、炭粒子１ｇに対して微生物の
数として１０３以上であればよく、通常１０５程度存在
すれば充分目的を達成できる。炭粒子に対する担持方法
に制限はないが、例えば乾燥微生物製剤と炭粒子を振り
混ぜる程度で、充分担持させることができる。油は大き
くは動植物油と鉱物油とに区分できる。これは化学構造
上の差が基本的にあるが、微生物も油分を分解する能力
を持っていても必ずしも両方の油を共に分解するとは限
らない。従って、ここで述べる微生物もしくは微生物製
剤は単一菌により構成されていても複合菌で成り立って
いても良い。油を分解する微生物は沢山のものが知られ
ており、好気性菌、嫌気性菌、通性嫌気性菌、カビ、酵
母等がある。例えば、比較的多く見受けられるものとし
てはＡｃｈｒｏｍｏｂａｃｔｅｒ、 Ａｒｔｈｒｏｂａ
ｃｔｅｒ、 Ａｃｉｎｅｔｏｂａｃｔｅｒ、 Ｃｏｒｙ
ｎｅｂａｃｔｅｒｉｕｍ、 Ｍｉｃｒｏｃｏｃｃｕｓ、
Ｐｓｅｕｄｏｍｏｎａｓ、 Ｆｌａｂｏｂａｃｔｅｒ
ｉｕｍ、 Ｍｙｃｏｂａｃｔｅｒｉｕｍ、 Ｎｏｃａｒ
ｄｉａ、 Ａｅｒｏｍｏｎａｓ、 Ｂａｃｉｌｌｕｓ、
Ｍｏｒａｘｅｌｌａ、 Ａｌｃａｌｉｇｅｎｅｓ、
Ａｃｔｉｎｏｍｙｃｅｓ、 Ｐｅｎｉｃｉｌｌｉｕｍ、
Ｃｕｎｎｉｎｇｈａｍｅｌｌａ、 Ｂｒｅｖｉｂａｃ
ｔｅｒｉｕｍ、 Ｒｈｏｄｏｃｏｃｃｕｓ、 Ｒｈｏｄ
ｏｔｏｒｕｌａ、 Ａｃｒｅｍｏｎｉｕｍ、 Ｂｅａｕ
ｖｅｒｉａ、 Ｃｌａｄｏｓｐｏｒｉｕｍ等多くの属の
微生物があり、沢山の成書〔石油発酵、石油発酵研究会
編、幸書房（１９７０年）、Ｐｅｔｒｏｌｅｕｍ Ｍｉ
ｃｒｏｂｉｏｌｏｇｙ、Ｊ．Ｂ．Ｄａｖｉｓ， Ｅｌｓ
ｅｖｉｅｒ Ｐｕｂｌｉｓｈｉｎｇ Ｃｏｍｐａｎｙ
（１９６７）、Ｅｎｖｉｒｏｎｍｅｎｔａｌ Ｂｉｏｔ
ｅｃｈｎｏｌｏｇｙ：Ｐｒｉｎｃｉｐｌｅｓ ａｎｄ
Ａｐｐｌｉｃａｔｉｏｎｓ， Ｍ． Ｍｏｏ−Ｙｏｕｎ
ｇ等編、Ｋｌｕｗｅｒ Ａｃａｄｅｍｉｃ Ｐｕｂｌｉ
ｓｈｅｒｓ（１９９６）， Ｒｅｍｅｄｉａｔｉｏｎ
ｏｆ Ｐｅｔｒｏｌｅｕｍ Ｃｏｎｔａｍｉｎａｔｅｄ
Ｓｏｉｌｓ， Ｅ． Ｒｉｓｅｒ−Ｒｏｂｅｒｔｓ，
Ｌｅｗｉｓ Ｐｕｂｌｉｓｈｅｒｓ（１９９８）〕を
参考にできる。The second feature of the present invention resides in that oil-absorbing charcoal, that is, charcoal particles having a bulk density of 0.8 or less, is allowed to coexist with a microorganism having an oil decomposing ability (including a microbial preparation). The amount of the microorganisms carried on the charcoal particles may be 10 3 or more as the number of microorganisms per 1 g of the charcoal particles, and the presence of about 10 5 is sufficient to achieve the purpose. The method for supporting the charcoal particles is not limited, but, for example, the dried microorganism preparation and the charcoal particles can be sufficiently supported by shaking. Oil can be roughly classified into animal and vegetable oils and mineral oils. This basically has a difference in chemical structure, but even if a microorganism has the ability to decompose oil, it does not always decompose both oils. Therefore, the microorganism or microbial preparation described here may be composed of a single bacterium or a complex bacterium. Many microorganisms that decompose oil are known, and there are aerobic bacteria, anaerobic bacteria, facultative anaerobic bacteria, molds, yeasts and the like. For example, Achromobacter and Arthroba are relatively common.
cter, Acinetobacter, Cory
nebacterium, Micrococcus,
Pseudomonas, Flabobacter
ium, Mycobacterium, Nocar
dia, Aeromonas, Bacillus,
Moraxella, Alcaligenes,
Actinomyces, Penicillium,
Cunninghamella, Brevibac
terium, Rhodococcus, Rhod
otorula, Acremonium, Beau
There are many genus of microorganisms such as Veria, Cladosporium, etc., and there are many books [Petroleum Fermentation, Petroleum Fermentation Research Society, edited by Saikoubo (1970), Petroleum Mi
crobiology, J. B. Davis, Els
evi Publishing Company
(1967), Environmental Biot.
technology: Principles and
Applications, M.M. Moo-Youn
G etc., Kluwer Academic Public
shers (1996), Remediation
of Petroleum Contaminated
Soils, E .; Riser-Roberts,
Lewis Publishers (1998)] can be referred to.
【０００９】請求項１〜３いずれか記載の微生物含有油
処理材は、通油性の袋に詰めて使用すれば、運送、取扱
い、吸油後の回収などの作業が便利に実施できる。通油
性の袋としては、不織布などで作ったものを例示するこ
とができる。例えば、Ａ４版、Ｂ４版などの大きさの封
筒状の袋に微生物含有油処理材をつめて使用する。袋に
詰めたときの袋の厚みは、とくに限定するものではない
が、０．５〜３ｃｍ程度である。When the microorganism-containing oil treatment material according to any one of claims 1 to 3 is packed in an oil-permeable bag and used, operations such as transportation, handling, and collection after oil absorption can be carried out conveniently. Examples of the oil-permeable bag include those made of non-woven fabric. For example, a microbe-containing oil treatment material is packed in an envelope-shaped bag having a size such as A4 size and B4 size before use. The thickness of the bag when packed in the bag is not particularly limited, but is about 0.5 to 3 cm.
【００１０】本発明において、微生物含有油吸収材に吸
収された油分を分解除去する条件は菌に適した好気的条
件あるいは嫌気的条件を採用する。油を分解するにあた
っては、微生物の活動を活発化するため、油が含有する
炭素以外の補助栄養源を添加することが好ましい。油中
の炭素以外に窒素とリン成分を添加する。さらに必要に
応じてＮａ、Ｋ、Ｍｇ、Ｃａ、Ｓ、Ｂ、Ｏ、Ｈ、Ｆｅ、
Ｃｏ、Ｃｕ、Ｍｏ、Ｚｎ、Ｍｎ等の元素成分の少なくと
も１種を与えると分解を促進できる。これら無機塩の組
成については沢山の文献が知られており、成書〔石油発
酵、石油発酵研究会編、幸書房（１９７０年）、Ｐｅｔ
ｒｏｌｅｕｍ Ｍｉｃｒｏｂｉｏｌｏｇｙ、Ｊ．Ｂ．Ｄ
ａｖｉｓ， Ｅｌｓｅｖｉｅｒ Ｐｕｂｌｉｓｈｉｎｇ
Ｃｏｍｐａｎｙ（１９６７）、Ｅｎｖｉｒｏｎｍｅｎ
ｔａｌ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ：Ｐｒｉｎｃｉｐ
ｌｅｓ ａｎｄ Ａｐｐｌｉｃａｔｉｏｎｓ， Ｍ．
Ｍｏｏ−Ｙｏｕｎｇ等編、Ｋｌｕｗｅｒ Ａｃａｄｅｍ
ｉｃ Ｐｕｂｌｉｓｈｅｒｓ（１９９６）， Ｒｅｍｅ
ｄｉａｔｉｏｎ ｏｆ Ｐｅｔｒｏｌｅｕｍ Ｃｏｎｔ
ａｍｉｎａｔｅｄ Ｓｏｉｌｓ， Ｅ． Ｒｉｓｅｒ−
Ｒｏｂｅｒｔｓ，Ｌｅｗｉｓ Ｐｕｂｌｉｓｈｅｒｓ
（１９９８）〕を参考にすることが出来る。In the present invention, the conditions for decomposing and removing the oil absorbed by the microorganism-containing oil absorbent are aerobic conditions or anaerobic conditions suitable for bacteria. In decomposing the oil, it is preferable to add a supplemental nutrient source other than carbon contained in the oil in order to activate the activity of microorganisms. In addition to carbon in oil, nitrogen and phosphorus components are added. If necessary, Na, K, Mg, Ca, S, B, O, H, Fe,
Decomposition can be promoted by providing at least one elemental component such as Co, Cu, Mo, Zn, and Mn. A lot of literatures are known about the composition of these inorganic salts, and they are published in the book [Petroleum Fermentation, Petroleum Fermentation Study Group, Koshobo (1970), Pet.
roleum Microbiology, J. Mol. B. D
avis, Elsevier Publishing
Company (1967), Environmen
tal Biotechnology: Princip
les and Applications, M.S.
Edited by Moo-Young, Kluwer Academ
ic Publishers (1996), Reme
direction of Petroleum Cont
aminated Oils, E.I. Riser-
Roberts, Lewis Publishers
(1998)] can be referred to.
【００１１】[0011]
【発明の実施の形態】以下に、実験例、実施例を挙げて
本発明を説明するが、本発明はこれにより何ら限定され
るものではない。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described below with reference to experimental examples and examples, but the present invention is not limited thereto.
【００１２】実験例１ 油分吸収炭〔表１の炭粒子
（イ）〕のＡ重油吸収試験
１０ｍｌの水に０．１ｍｌ、０．２ｍｌ、０．３ｍｌ及
び０．４ｍｌのＡ重油を加えた４つのケースのそれぞれ
に表１の炭粒子（イ）を０．１ｇづつ加えて激しく振
り、混和して１５時間放置し、水の油分濃度を測定し
た。ただし、０．４ｍｌでは油分は水面上に浮遊存在し
ているので水の油分濃度は測定しなかった。炭粒子添加
直後では０．３ｍｌのケースでは若干の油分が残った
が、０．４ｍｌのケースでは明らかに吸収されない油分
が肉眼ではっきりと分かった。１５時間後では０．３ｍ
ｌのところには油滴が観察されなかったのでその水中の
油分濃度も測定してみた。その結果を表２に示す。な
お、表１の炭粒子（ロ）、（ハ）、（ニ）についても、
同じ実験を行なったが、炭粒子（イ）とほぼ同様の結果
であった。Experimental Example 1 A heavy oil absorption test of oil-absorbing coal [charcoal particles (a) in Table 1] 0.1 ml, 0.2 ml, 0.3 ml and 0.4 ml of A heavy oil were added to 10 ml of water 4 To each of the two cases, 0.1 g of the charcoal particles (a) in Table 1 was added, shaken vigorously, mixed and allowed to stand for 15 hours, and the oil content of water was measured. However, at 0.4 ml, the oil content was floating on the water surface, so the oil content concentration of water was not measured. Immediately after the addition of the charcoal particles, some oil remained in the case of 0.3 ml, but in the case of 0.4 ml, the oil that was not clearly absorbed was clearly visible to the naked eye. 0.3m after 15 hours
No oil droplet was observed at l, so the oil concentration in the water was also measured. The results are shown in Table 2. In addition, regarding the carbon particles (b), (c), and (d) in Table 1,
The same experiment was performed, but the result was almost the same as that of the charcoal particle (a).
【００１３】[0013]
【表２】 [Table 2]
【００１４】実験例２
実験例１と同様な試験をガソリン、灯油、軽油、菜種油
についても行った。これらの油種においても０．３ｍｌ
のところでは肉眼的には炭粒子添加後瞬時で油滴が観察
されなくなった。０．４ｍｌのところは肉眼的に油滴が
観察されたので油分の測定はしなかった。残油量の測定
結果を表３に示す。なお、表１の炭粒子（ロ）、
（ハ）、（ニ）についても同じ実験を行なったが、炭粒
子（イ）とほぼ同様の結果であった。Experimental Example 2 The same tests as in Experimental Example 1 were conducted on gasoline, kerosene, light oil and rapeseed oil. 0.3 ml for these oil types
At this point, no oil droplets were visually observed immediately after the addition of the charcoal particles. Since oil droplets were visually observed at 0.4 ml, the oil content was not measured. Table 3 shows the measurement results of the residual oil amount. In addition, the charcoal particles (b) in Table 1,
The same experiment was conducted for (c) and (d), but the results were almost the same as for the carbon particles (a).
【００１５】[0015]
【表３】
鉱物油のみならず植物油でも良く吸収することが明らか
となった。植物油の方が残油量が多いのは植物油が鉱物
油に比べて極性が高く、その分水への溶解性が高いため
と考えられる。[Table 3] It was revealed that it is well absorbed not only by mineral oil but also by vegetable oil. It is considered that the amount of residual oil in vegetable oil is higher because vegetable oil is more polar than mineral oil and its solubility in water is higher.
【００１６】実施例１ 微生物含有油処理材上での微生
物による分解試験Ｒｈｏｄｏｃｏｃｃｕｓ ｓｐ（飽和
炭化水素を良く分解する）及びＡｃｉｎｅｔｏｂａｃｔ
ｅｒ ｓｐ（芳香族炭化水素を良く分解する）を珪藻土
に吸着させた微生物製剤（ＲＢ）０．１ｇを表１の炭粒
子（イ）２ｇに添加し、良く振り混ぜて混和することに
より、微生物製剤が炭に良く付着した微生物含有油処理
材を作った。この微生物含有油処理材２ｇにＡ重油１ｇ
あるいは軽油１ｇを加え、そこへ下記補助栄養源水溶液
を０日目、７日目及び１４日目に夫々１ｍｌづつ添加し
た。なお、この間、系は室温（１５〜２５℃）に保っ
た。Example 1 Microbial Decomposition Test on Microorganism-Containing Oil Treatment Material Rhodococcus sp (which decomposes saturated hydrocarbons well) and Acinetact
er sp (which decomposes aromatic hydrocarbons well) adsorbed on diatomaceous earth (RB) 0.1 g was added to charcoal particles (a) 2 g in Table 1 and mixed well by shaking to mix the microorganisms. A microbial-containing oil treatment material was prepared whose formulation adhered well to charcoal. 2g of this oil-containing material containing microorganisms
Alternatively, 1 g of light oil was added, and 1 ml of the following supplemental nutrient source aqueous solution was added to each of them on days 0, 7, and 14. During this period, the system was kept at room temperature (15 to 25 ° C).
【００１７】補助栄養源水溶液の組成例は下記表４に示
すが、これに限定されるものではない。A composition example of the supplemental nutrient source aqueous solution is shown in Table 4 below, but is not limited thereto.
【００１８】[0018]
【表４】 [Table 4]
【００１９】時間ごとに試料を四塩化炭素１０ｍｌで抽
出後、シリカカラム処理を経て赤外分光器で油分濃度を
測定した。その結果を図１および図２に示す。分解速度
が極めて速いことが判る。After the sample was extracted with 10 ml of carbon tetrachloride every hour, it was treated with a silica column and the oil concentration was measured with an infrared spectroscope. The results are shown in FIGS. 1 and 2. It can be seen that the decomposition rate is extremely fast.
【００２０】実施例２ 微生物含有油処理材上での微生
物による分解試験
微生物としてＡｃｉｎｅｔｏｂａｃｔｅｒ ｓｐ（Ａ）
を炭粒子（イ）上で１０５個／ｇとなるように添加し実
施例１の要領で作った微生物含有油処理材２ｇに菜種油
１ｇを加え、そこへ前記補助栄養源水溶液を（各０、
７、１４日）実施例１と同じ要領で加えて油分の分解経
過を調べた。系の反応温度は室温（１５〜２５℃）で行
った。前記補助栄養源水溶液を０日目、７日目及び１４
日目と夫々１ｍｌを添加した。なお、この間、系は室温
（１５〜２５℃）に保った。所定時間ごとに試料を四塩
化炭素１０ｍｌで抽出後、シリカカラム処理を経て赤外
分光器で油分濃度を測定した。その時の結果は図３に示
す通りであった。この場合も分解速度が極めて速いこと
が判る。Example 2 Microbial Degradation Test on Microbial-Containing Oil Treatment Material Acinetobacter sp (A) was used as a microorganism.
1 g of rapeseed oil was added to 2 g of the microorganism-containing oil treatment material prepared in the same manner as in Example 1 by adding 10 5 pieces / g on charcoal particles (a), and the supplemental nutrient source aqueous solution (0 each) was added thereto. ,
(7, 14 days) In the same manner as in Example 1, the decomposition process of oil was examined. The reaction temperature of the system was room temperature (15 to 25 ° C.). The above-mentioned supplemental nutrient source aqueous solution was applied on days 0, 7 and 14
On day one, 1 ml was added each. During this period, the system was kept at room temperature (15 to 25 ° C). The sample was extracted with carbon tetrachloride (10 ml) every predetermined time, treated with a silica column, and the oil concentration was measured by an infrared spectroscope. The result at that time was as shown in FIG. Also in this case, it can be seen that the decomposition rate is extremely high.
【００２１】[0021]
【発明の効果】１）水上に浮遊している油分を短時間で
吸収除去できる。炭粒子に吸収させた後は、吸収された
油分が他へ付着することがほとんどない。地上部でのこ
ぼした油分等も同様に吸収除去できる。
２）油吸収炭の嵩密度が小さいので、油を吸収する以前
に波間に沈むことがなく、また油分を吸収した炭は水上
に浮遊しているので、水との分離は容易で汚染油の回収
・除去を容易にする。
３）油を吸収した本発明の微生物含有油処理材に窒素、
リン等の補助栄養素を加えると、効率よく油を分解する
ことができる。
４）油分を吸収させて回収した炭は微生物により油分を
分解除去できるので産業廃棄物としての処理量を削減で
きる。
５）炭そのものは土壌改良剤としても利用されており、
微生物含有油吸収材による汚染油の除去・処理は自然環
境を守ることとなる。[Effects of the Invention] 1) Oil floating on water can be absorbed and removed in a short time. After being absorbed by the charcoal particles, the absorbed oil hardly adheres to others. Spilled oil on the ground can be absorbed and removed as well. 2) Since the bulk density of the oil-absorbing coal is small, it does not sink into the waves before absorbing the oil, and the oil-absorbing coal floats on the water, so it is easy to separate it from the water and Facilitates collection and removal. 3) Nitrogen is added to the oil treatment material containing microorganisms of the present invention which has absorbed oil,
Addition of supplemental nutrients such as phosphorus can efficiently decompose oil. 4) The charcoal recovered by absorbing oil can be decomposed and removed by microorganisms, so that the amount of industrial waste can be reduced. 5) Charcoal itself is also used as a soil conditioner,
The removal and treatment of contaminated oil with a microorganism-containing oil absorbent will protect the natural environment.
【図面の簡単な説明】[Brief description of drawings]
【図１】実施例１における油吸収材上での分解試験結果
を示す。縦軸は、油濃度、横軸は経過時間である。FIG. 1 shows the results of a decomposition test on an oil absorbent in Example 1. The vertical axis represents the oil concentration and the horizontal axis represents the elapsed time.
【図２】実施例１における油吸収材上での分解試験結果
を示す。縦軸は、残存油割合、横軸は経過時間である。FIG. 2 shows the results of a decomposition test on an oil absorbent in Example 1. The vertical axis is the residual oil ratio, and the horizontal axis is the elapsed time.
【図３】実施例２における油吸収材上での分解試験結果
を示す。縦軸は、残存油割合、横軸は経過時間である。FIG. 3 shows the results of a decomposition test on an oil absorbent in Example 2. The vertical axis is the residual oil ratio, and the horizontal axis is the elapsed time.
───────────────────────────────────────────────────── フロントページの続き Ｆターム(参考） 4B065 AA04X AA45X BB01 BB10 CA56 4D003 AA06 AA12 EA25 4D024 AA04 AA05 AB06 BA03 BB01 DB14 4D051 AA01 AB07 EA08 EB03 EC03 EC19 ─────────────────────────────────────────────────── ─── Continued front page F term (reference) 4B065 AA04X AA45X BB01 BB10 CA56 4D003 AA06 AA12 EA25 4D024 AA04 AA05 AB06 BA03 BB01 DB14 4D051 AA01 AB07 EA08 EB03 EC03 EC19
Claims (6)
Ｓ Ｋ１４７４により測定）０．８以下の炭粒子に、油
分解能を有する微生物（微生物製剤も含む）を担持させ
たことを特徴とする微生物含有油処理材。1. Bulk density (filling density, Japanese Industrial Standard JI
A microbial-containing oil treatment material, characterized in that a carbon particle of 0.8 or less (measured by SK1474) is loaded with a microorganism having an oil degrading property (including a microbial preparation).
請求項１記載の微生物含有油処理材。2. The microorganism-containing oil treatment material according to claim 1, wherein the coal particles have a bulk density of 0.7 or less.
請求項１記載の微生物含有油処理材。3. The microorganism-containing oil treatment material according to claim 1, wherein the carbon particles have a bulk density of 0.5 or less.
３いずれか記載の微生物含有油処理材。4. The method according to claim 1, wherein the bag is packed in an oil-permeable material bag.
3. The microorganism-containing oil treatment material according to any one of 3 above.
油処理材に油を吸収させた後、これを回収し、微生物に
より分解することを特徴とする油の処理方法。5. A method for treating oil, which comprises absorbing oil with the microorganism-containing oil treatment material according to any one of claims 1 to 4, recovering the oil, and decomposing it with microorganisms.
う請求項５記載の油の処理方法。6. The method for treating oil according to claim 5, wherein the decomposition of microorganisms is carried out in the presence of a supplemental nutrient source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001252063A JP2003061643A (en) | 2001-08-22 | 2001-08-22 | Oil-treating material containing microorganism and method for treating oil by using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001252063A JP2003061643A (en) | 2001-08-22 | 2001-08-22 | Oil-treating material containing microorganism and method for treating oil by using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2003061643A true JP2003061643A (en) | 2003-03-04 |
Family
ID=19080603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001252063A Pending JP2003061643A (en) | 2001-08-22 | 2001-08-22 | Oil-treating material containing microorganism and method for treating oil by using the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2003061643A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006090859A1 (en) * | 2005-02-25 | 2006-08-31 | National University Corporation Tottori University | Method of recycling water-soluble processed liquid, apparatus for recycling water-soluble processed liquid, method of treating oil-containing wastewater and apparatus for treating oil-containing wastewater |
| GB2399567B (en) * | 2003-03-15 | 2006-10-25 | Permavoid Ltd | Storage module |
| JP2007039954A (en) * | 2005-08-02 | 2007-02-15 | Asenthy:Kk | Oil absorber using oil decomposing microorganism and biodegradation nonwoven fabric |
| JP2011057968A (en) * | 2009-08-12 | 2011-03-24 | Fuji Mentenir Kk | Agent for treating oils, method for producing such treatment agent, and method for treating oils using the treatment agent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1099851A (en) * | 1996-09-27 | 1998-04-21 | Taniguchi Shokai Kk | Oil absorbing material and oil absorbing bag |
| JPH1128488A (en) * | 1997-07-14 | 1999-02-02 | Kuraray Chem Corp | Waste water treatment device and treatment of waste water |
| JPH1177074A (en) * | 1997-07-14 | 1999-03-23 | Kuraray Chem Corp | Device and method for treating wastewater |
-
2001
- 2001-08-22 JP JP2001252063A patent/JP2003061643A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1099851A (en) * | 1996-09-27 | 1998-04-21 | Taniguchi Shokai Kk | Oil absorbing material and oil absorbing bag |
| JPH1128488A (en) * | 1997-07-14 | 1999-02-02 | Kuraray Chem Corp | Waste water treatment device and treatment of waste water |
| JPH1177074A (en) * | 1997-07-14 | 1999-03-23 | Kuraray Chem Corp | Device and method for treating wastewater |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2399567B (en) * | 2003-03-15 | 2006-10-25 | Permavoid Ltd | Storage module |
| WO2006090859A1 (en) * | 2005-02-25 | 2006-08-31 | National University Corporation Tottori University | Method of recycling water-soluble processed liquid, apparatus for recycling water-soluble processed liquid, method of treating oil-containing wastewater and apparatus for treating oil-containing wastewater |
| JPWO2006090859A1 (en) * | 2005-02-25 | 2008-07-24 | 国立大学法人鳥取大学 | Water-soluble processing fluid recycling method, water-soluble processing fluid recycling device, oil-containing wastewater treatment method, and oil-containing wastewater treatment device |
| JP5119441B2 (en) * | 2005-02-25 | 2013-01-16 | 国立大学法人鳥取大学 | Water-soluble processing fluid recycling method, water-soluble processing fluid recycling device, oil-containing wastewater treatment method, and oil-containing wastewater treatment device |
| JP2007039954A (en) * | 2005-08-02 | 2007-02-15 | Asenthy:Kk | Oil absorber using oil decomposing microorganism and biodegradation nonwoven fabric |
| JP2011057968A (en) * | 2009-08-12 | 2011-03-24 | Fuji Mentenir Kk | Agent for treating oils, method for producing such treatment agent, and method for treating oils using the treatment agent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Agarry et al. | Kinetic modelling and half-life study on enhanced soil bioremediation of bonny light crude oil amended with crop and animal-derived organic wastes | |
| Al-Asheh et al. | Beneficial reuse of chicken feathers in removal of heavy metals from wastewater | |
| CA2257706C (en) | Process and composition for treating hydrocarbon contaminated material | |
| US7166221B1 (en) | Oil digesting microbe-plastic foam system | |
| Agarry et al. | Application of carbon-nitrogen supplementation from plant and animal sources in in-situ soil bioremediation of diesel oil: experimental analysis and kinetic modelling | |
| Dedov et al. | New materials and ecology: Biocomposites for aquatic remediation | |
| Kalbuadi et al. | The potential use of natural clinoptilolite zeolite for crude oil spill removal from sea water | |
| Mani et al. | Microbes mediated approaches for environmental waste management | |
| Sozina et al. | Microbiological remediation of oil-contaminated soils | |
| JP2003061643A (en) | Oil-treating material containing microorganism and method for treating oil by using the same | |
| Biswas et al. | Microbial uptake of diesel oil sorbed on soil and oil spill clean‐up sorbents | |
| Antwi-Akomeah et al. | Hydrocarbon contaminated water remediation using a locally constructed multi-stage bioreactor incorporated with media filtration. | |
| Kuo et al. | Using enhanced landfarming system to remediate diesel oil-contaminated soils | |
| Margesin et al. | A feasibility study for the in situ remediation of a former tank farm | |
| Mathew et al. | Bioremediation of diesel oil in marine environment | |
| JP2003010834A (en) | Bioremediation method for polluted soil | |
| Bordoloi et al. | Analysis of recent patenting activities in the field of bioremediation of petroleum hydrocarbon pollutants present in the environment | |
| AU767753B2 (en) | Process and composition for treating hydrocarbon contaminated material | |
| KR100455754B1 (en) | Biological-biodegrading adsorbent for removing hydrocarbon compounds such as oil and method for manufacturing thereof | |
| Fokina et al. | Use of mineral sorbents for the purification of natural environments from petroleum products in the Kolsky northern region | |
| US6780631B2 (en) | Method and apparatus for decomposition of oil | |
| Lisichkin et al. | Elimination of emergency oil spills: state of the art and problems | |
| Chekroud et al. | Biodegradation of crude oil in marine medium | |
| JP4421254B2 (en) | Recycling method of oil adsorbent | |
| Binsadiq | Fungal biodegradation of diesel by Ulocladium atrum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20080813 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100510 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110315 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20110531 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110830 |
|
| A911 | Transfer of reconsideration by examiner before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20110913 |
|
| A912 | Removal of reconsideration by examiner before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A912 Effective date: 20111118 |