JP2002531127A5 - - Google Patents
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- JP2002531127A5 JP2002531127A5 JP2000586908A JP2000586908A JP2002531127A5 JP 2002531127 A5 JP2002531127 A5 JP 2002531127A5 JP 2000586908 A JP2000586908 A JP 2000586908A JP 2000586908 A JP2000586908 A JP 2000586908A JP 2002531127 A5 JP2002531127 A5 JP 2002531127A5
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- JP
- Japan
- Prior art keywords
- cells
- uhvec
- haosmc
- hours
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000004027 cells Anatomy 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 210000002889 Endothelial Cells Anatomy 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 210000002966 Serum Anatomy 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 210000003606 Umbilical Veins Anatomy 0.000 description 1
- 210000003433 aortic smooth muscle cell Anatomy 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000001605 fetal Effects 0.000 description 1
- 239000002609 media Substances 0.000 description 1
- 230000002297 mitogenic Effects 0.000 description 1
- 230000031055 positive regulation of mitosis Effects 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
Description
実施例21．
組換えzvegf3を、ヒト大動脈平滑筋細胞（HAoSMC；Clontech Corp.、マリイランド州ウォークスビレ）およびヒト臍静脈内皮細胞（UHVEC；Clontech Corp.）に対する細胞***促進活性について分析した。HAoSMCおよびUHVECを96ウェルの培養プレートの中に5,000細胞／ウェルの密度でプレートし、10％のウシ胎児血清を含有するDMEM中で37℃においてほぼ24時間成長させた。インスリン（5μg／ml）、トランスフェリン（20μg／ml）、およびセレン（16pg／ml）を含有する無血清DMEM／Ham's F−12（ITS）中で細胞を24時間インキュベートすることによって、細胞を休止させた。アッセイ時に、培地を除去し、試験試料をウェルにトリプリケートで添加した。
組換えzvegf3を、ヒト大動脈平滑筋細胞（HAoSMC；Clontech Corp.、マリイランド州ウォークスビレ）およびヒト臍静脈内皮細胞（UHVEC；Clontech Corp.）に対する細胞***促進活性について分析した。HAoSMCおよびUHVECを96ウェルの培養プレートの中に5,000細胞／ウェルの密度でプレートし、10％のウシ胎児血清を含有するDMEM中で37℃においてほぼ24時間成長させた。インスリン（5μg／ml）、トランスフェリン（20μg／ml）、およびセレン（16pg／ml）を含有する無血清DMEM／Ham's F−12（ITS）中で細胞を24時間インキュベートすることによって、細胞を休止させた。アッセイ時に、培地を除去し、試験試料をウェルにトリプリケートで添加した。
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20712098A | 1998-12-07 | 1998-12-07 | |
| US09/207,120 | 1998-12-07 | ||
| US14257699P | 1999-07-06 | 1999-07-06 | |
| US60/142,576 | 1999-07-06 | ||
| US16165399P | 1999-10-21 | 1999-10-21 | |
| US60/161,653 | 1999-10-21 | ||
| US16525599P | 1999-11-12 | 1999-11-12 | |
| US60/165,255 | 1999-11-12 | ||
| PCT/US1999/028968 WO2000034474A2 (en) | 1998-12-07 | 1999-12-07 | Growth factor homolog zvegf3 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010254489A Division JP5345997B2 (ja) | 1998-12-07 | 2010-11-15 | 成長因子相同体ｚｖｅｇｆ３ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002531127A JP2002531127A (ja) | 2002-09-24 |
| JP2002531127A5 true JP2002531127A5 (ja) | 2011-02-17 |
| JP4739526B2 JP4739526B2 (ja) | 2011-08-03 |
Family
ID=27495530
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000586908A Expired - Fee Related JP4739526B2 (ja) | 1998-12-07 | 1999-12-07 | 成長因子相同体ｚｖｅｇｆ３ |
| JP2010254489A Expired - Fee Related JP5345997B2 (ja) | 1998-12-07 | 2010-11-15 | 成長因子相同体ｚｖｅｇｆ３ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010254489A Expired - Fee Related JP5345997B2 (ja) | 1998-12-07 | 2010-11-15 | 成長因子相同体ｚｖｅｇｆ３ |
Country Status (9)
| Country | Link |
|---|---|
| EP (2) | EP1137773B1 (ja) |
| JP (2) | JP4739526B2 (ja) |
| AT (1) | ATE404668T1 (ja) |
| AU (1) | AU764039B2 (ja) |
| CA (1) | CA2354325C (ja) |
| DE (1) | DE69939330D1 (ja) |
| DK (1) | DK1137773T3 (ja) |
| ES (2) | ES2432053T3 (ja) |
| WO (1) | WO2000034474A2 (ja) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| EP1064382B1 (en) | 1998-03-17 | 2008-08-20 | Genentech, Inc. | Polypeptides homologous to vegf and bmp1 |
| ATE556090T1 (de) * | 1998-11-10 | 2012-05-15 | Ludwig Inst Cancer Res | Verkürzter, von blutplättchen abstammender wachstumsfaktor d, dafür kodierendes dns und deren verwendung |
| US7148037B2 (en) | 1998-11-10 | 2006-12-12 | Ludwig Institute For Cancer Research | Platelet-derived growth factor D, DNA coding therefor, and uses thereof |
| US7105481B2 (en) | 1998-11-10 | 2006-09-12 | Ludwig Institute For Cancer Research | Method for stimulating connective tissue growth or wound healing |
| AU3514400A (en) * | 1999-03-08 | 2000-09-28 | Genentech Inc. | Compositions and methods for the treatment of immune related diseases |
| WO2000059940A2 (en) * | 1999-04-06 | 2000-10-12 | Eli Lilly And Company | Platelet-derived growth factor related gene and protein |
| US6630142B2 (en) | 1999-05-03 | 2003-10-07 | Zymogenetics, Inc. | Method of treating fibroproliferative disorders |
| US6468543B1 (en) | 1999-05-03 | 2002-10-22 | Zymogenetics, Inc. | Methods for promoting growth of bone using ZVEGF4 |
| AU4714500A (en) * | 1999-05-03 | 2000-11-17 | Zymogenetics Inc. | Growth factor homolog zvegf4 |
| EP1222279A2 (en) * | 1999-10-07 | 2002-07-17 | Curagen Corporation | Growth factor polypeptides and nucleic acids encoding same |
| US6893637B1 (en) | 1999-10-21 | 2005-05-17 | Zymogenetics, Inc. | Method of treating fibrosis |
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| WO2001057083A1 (en) * | 2000-02-04 | 2001-08-09 | Zymogenetics, Inc. | Methods for promoting growth of bone, ligament, and cartilage using zvegf4 |
| US20040086507A1 (en) * | 2000-10-19 | 2004-05-06 | Kenya Shitara | Antibody inhibiting vplf activity |
| JP2004527506A (ja) * | 2001-03-09 | 2004-09-09 | ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ | 平滑筋細胞増殖のモジュレーション |
| WO2003065043A2 (en) * | 2002-01-29 | 2003-08-07 | King's College London An Institute Incorporated By Royal Charter Of Strand | Identification and relative quantification of proteins |
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| ATE432716T1 (de) * | 2003-07-25 | 2009-06-15 | Zymogenetics Inc | Verfahren zur behandlung von hepatozellulärem karzinom |
| US8629836B2 (en) | 2004-04-30 | 2014-01-14 | Hillcrest Laboratories, Inc. | 3D pointing devices with orientation compensation and improved usability |
| US7239301B2 (en) | 2004-04-30 | 2007-07-03 | Hillcrest Laboratories, Inc. | 3D pointing devices and methods |
| US8137195B2 (en) | 2004-11-23 | 2012-03-20 | Hillcrest Laboratories, Inc. | Semantic gaming and application transformation |
| CA2871528A1 (en) | 2012-04-24 | 2013-10-31 | Thrombogenics N.V. | Anti-pdgf-c antibodies |
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-
1999
- 1999-12-07 DK DK99966032T patent/DK1137773T3/da active
- 1999-12-07 ES ES08075507T patent/ES2432053T3/es not_active Expired - Lifetime
- 1999-12-07 WO PCT/US1999/028968 patent/WO2000034474A2/en active IP Right Grant
- 1999-12-07 JP JP2000586908A patent/JP4739526B2/ja not_active Expired - Fee Related
- 1999-12-07 ES ES99966032T patent/ES2312223T3/es not_active Expired - Lifetime
- 1999-12-07 EP EP99966032A patent/EP1137773B1/en not_active Expired - Lifetime
- 1999-12-07 DE DE69939330T patent/DE69939330D1/de not_active Expired - Lifetime
- 1999-12-07 AT AT99966032T patent/ATE404668T1/de active
- 1999-12-07 CA CA2354325A patent/CA2354325C/en not_active Expired - Fee Related
- 1999-12-07 AU AU21679/00A patent/AU764039B2/en not_active Ceased
- 1999-12-07 EP EP08075507.7A patent/EP1988102B1/en not_active Expired - Lifetime
-
2010
- 2010-11-15 JP JP2010254489A patent/JP5345997B2/ja not_active Expired - Fee Related
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