JP2002336678A - Liquid transferring container - Google Patents
Liquid transferring containerInfo
- Publication number
- JP2002336678A JP2002336678A JP2001141450A JP2001141450A JP2002336678A JP 2002336678 A JP2002336678 A JP 2002336678A JP 2001141450 A JP2001141450 A JP 2001141450A JP 2001141450 A JP2001141450 A JP 2001141450A JP 2002336678 A JP2002336678 A JP 2002336678A
- Authority
- JP
- Japan
- Prior art keywords
- container
- electrode
- sample
- polarity
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Electrostatic Separation (AREA)
- Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は微量の荷電物質を取
り扱う液体移送器に関するものである。本発明の液体移
送器は、例えば微量の溶液を反応容器などから微量分析
装置（マイクロチップ電気泳動装置など）に供給するの
に適している。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid transfer device for handling a trace amount of a charged substance. The liquid transfer device of the present invention is suitable for supplying, for example, a trace amount of a solution from a reaction container or the like to a trace amount analyzer (such as a microchip electrophoresis device).
【０００２】[0002]
【従来の技術】従来、微量の液体サンプルを取り扱う
際、容器として例えば９６穴や３８４穴などのマイクロ
タイタープレートのウエルを用いており、ウエル内のサ
ンプルを取り扱う液体移送器としては、シリンジなどの
吸引吐出機構により管状のノズル内にサンプルを出し入
れする分注器を用いている。また、近年、容器容量にあ
わせた反応スケールだと試薬代が大きな負担となるの
で、例えば種々のスクリーニングやジェノタイピング
（遺伝子型解析）等においては、反応スケールの微量化
（ナノリットルオーダー）が進んでいる。したがって、
微量のサンプルを扱う液体移送器の重要性が増してい
る。2. Description of the Related Art Conventionally, when handling a small amount of a liquid sample, a well of a microtiter plate having, for example, 96 holes or 384 holes is used as a container, and a liquid transfer device such as a syringe is used as a liquid transfer device for handling the sample in the well. A dispenser is used to take a sample in and out of a tubular nozzle by a suction and discharge mechanism. Further, in recent years, a reagent scale becomes a large burden when a reaction scale is adapted to the capacity of a container. Therefore, for example, in various screenings and genotyping (genotyping analysis), the reaction scale is reduced to a small amount (on the order of nanoliters). In. Therefore,
The importance of liquid transporters for handling small amounts of sample is increasing.
【０００３】[0003]
【発明が解決しようとする課題】しかし、シリンジを使
用した従来の液体移送器では、シリンジ容量を起因とす
る微量化対応への限界がある。また、微量なサンプル
は、表面張力などの影響を受けるため一定量の溶液を移
し替える事が困難である。さらに、溶液に含まれる不純
物、泡の除去、容器に移し替える際の泡の発生の防止な
どが困難である。そこで、本発明は、上記課題を解決
し、微量の溶液を取り扱うことができる液体移送器を提
供することを目的とする。However, the conventional liquid transfer device using a syringe has a limitation in miniaturization due to the capacity of the syringe. Further, since a small amount of sample is affected by surface tension and the like, it is difficult to transfer a certain amount of solution. Further, it is difficult to remove impurities and bubbles contained in the solution and to prevent the generation of bubbles when the solution is transferred to a container. Therefore, an object of the present invention is to solve the above-mentioned problems and to provide a liquid transfer device that can handle a small amount of solution.
【０００４】[0004]
【課題を解決するための手段】本発明は、上記課題を解
決するため、開口を有し、荷電物質を吸引するための吸
引部材と、前記吸引部材内に収容される電極と、前記電
極の極性を切換える極性反転器とからなる液体移送器を
提供する。ここで、吸引部材は、細管、多孔質体、ゲル
などで構成することができるが、細管が好ましい。細管
の先端の内径（開口径）は、１〜５００μｍ、長さは、
５〜５０ｍｍが好ましい。なお、細管には、試料との電
気導通を可能とするため、電解液あるいはゲルが表面張
力により管内に保持されることが好ましい。電解液とし
ては、例えばトリス−ホウ酸バッファ、りん酸バッファ
などを用いることができ、ゲルとしては、例えばポリア
クリルアミドゲルなどを用いることができるが、これら
に限定されず、通常使用される電気泳動バッファ、媒体
を利用することができる。なお、電解液あるいはゲルは
多孔質素材に吸収させて細管内に収容させてもよい。電
極は、例えば白金、アルミなどの金属電極を用いること
ができる。極性反転器は、例えば極性の異なる電源を複
数用意してそれを切換えるもの、電源が１つで回路内の
接続スイッチを切換えるものなど周知の極性反転器を用
いることができる。In order to solve the above-mentioned problems, the present invention provides a suction member having an opening for sucking a charged substance, an electrode housed in the suction member, Provided is a liquid transfer device including a polarity inverter for switching polarity. Here, the suction member can be formed of a thin tube, a porous body, a gel, or the like, but a thin tube is preferable. The inner diameter (opening diameter) of the tip of the thin tube is 1 to 500 μm, and the length is
5 to 50 mm is preferred. In addition, it is preferable that an electrolytic solution or a gel is held in the thin tube by surface tension in order to enable electrical conduction with the sample. As the electrolytic solution, for example, a tris-borate buffer, a phosphate buffer, or the like can be used. As the gel, for example, a polyacrylamide gel can be used, but not limited thereto. Buffers and media can be used. The electrolyte or the gel may be absorbed in a porous material and stored in a thin tube. As the electrode, for example, a metal electrode such as platinum or aluminum can be used. As the polarity inverter, for example, a known polarity inverter such as one that prepares a plurality of power supplies having different polarities and switches between them, or one that switches a connection switch in a circuit with one power supply can be used.
【０００５】また、本発明は、液体サンプルを収容又は
吐出する容器と、該容器内に挿入する前述の液体移送器
と、前記容器内のサンプルの電荷を前記液体移送器内の
極性と逆の極性に荷電させる電界手段とを備えてなる液
体吸引・吐出装置をも提供する。ここで、容器は、例え
ばビーカーのみならず、一表面に１又は複数の窪みが形
成されているプレート状のものなど何でもよい。この容
器内に電極を挿入するか、或いは容器自体に電極を刻設
する。また、吐出先は容器に限定されず、例えば、マイ
クロチップ電気泳動装置などの分析装置の試料リザーバ
ーに直接吐出させてもよい。さらに、吸引・吐出する液
体サンプルの量は、電極に印加する電界強度及び電界を
かける時間の制御により行うことができる。Further, the present invention provides a container for containing or discharging a liquid sample, the above-described liquid transfer device inserted into the container, and the charge of the sample in the container having the opposite polarity to the polarity in the liquid transfer device. There is also provided a liquid suction / discharge device including an electric field means for charging to a polarity. Here, the container is not limited to a beaker, for example, and may be a plate-like container having one or more depressions formed on one surface. The electrode is inserted into the container or the electrode is engraved on the container itself. Further, the discharge destination is not limited to the container, and for example, may be directly discharged to a sample reservoir of an analyzer such as a microchip electrophoresis apparatus. Further, the amount of the liquid sample to be sucked / discharged can be controlled by controlling the electric field intensity applied to the electrodes and the time for applying the electric field.
【０００６】[0006]
【発明の実施の形態】本発明の液体移送器の一実施例を
図面に基いて説明する。図１は一部断面を示す図で、１
は細管で、先端部は鋭利化され漏斗状となっている。先
端部の内径は、１〜５００μｍである。細管１内には電
解液（例えばトリス−ホウ酸バッファ）４が収容されて
おり、この電解液４は表面張力により細管内に保持され
ている。また、２は電極であり、電極２は電源３と接続
している。さらに図示しないサンプル容器内に電極６、
６’が収容されており、その電極６、６’とで回路を形
成している。回路には切換スイッチ５、５’が設けてあ
り、切換スイッチ５、５’を切換えることにより、電極
の極性を反転できる。なお、細管１の上部は、図示しな
いが細管保持部材により保持されており、細管保持部材
は圧電素子、ラックピ二オン機構などにより上下、左右
に移動される。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the liquid transfer device of the present invention will be described with reference to the drawings. FIG. 1 shows a partial cross section.
Is a thin tube, the tip of which is sharpened and has a funnel shape. The inner diameter of the tip is 1 to 500 μm. An electrolytic solution (for example, a tris-borate buffer) 4 is contained in the thin tube 1, and the electrolytic solution 4 is held in the thin tube by surface tension. Reference numeral 2 denotes an electrode, and the electrode 2 is connected to a power supply 3. Further, an electrode 6,
6 'is housed, and the electrodes 6 and 6' form a circuit. The circuit is provided with changeover switches 5, 5 ', and by switching the changeover switches 5, 5', the polarity of the electrodes can be inverted. The upper portion of the thin tube 1 is held by a thin tube holding member (not shown), and the thin tube holding member is moved vertically and horizontally by a piezoelectric element, a rack and pinion mechanism, or the like.
【０００７】以上の構成で、マイナス荷電物質を対象と
したサンプラー使用例を説明する。図２（a）はサンプ
ルの吸引を説明する図、図２（ｂ）はサンプルの吐出を
説明する図である。容器Ａ内に液体サンプルＳおよび電
極６が収容され、容器Ｂには電解液および電極６’が収
容されており、容器Ａ内の液体サンプルの目的物質を容
器Ｂに吐出させる。液体サンプルＳに含まれるマイナス
に荷電した対象物質は、細管１を液体サンプルＳにつけ
た状態で、細管１内の電極２をプラスに、容器Ａ内の電
極６をマイナスに荷電することにより、目的物質はその
極性から、細管１内へ流入する。この際、電荷を持たな
い物質は移動しないため、目的物質と同じ極性を持った
物質のみ細管１内へ取り込まれ、極性を持たない物質及
び極性が異なる物質は排除される。したがって、本発明
では、サンプルの吸引とともにサンプルの精製も同時に
行える。An example of using a sampler having the above configuration for a negatively charged substance will be described. FIG. 2A is a diagram illustrating suction of a sample, and FIG. 2B is a diagram illustrating ejection of a sample. The container A contains the liquid sample S and the electrode 6, and the container B contains the electrolytic solution and the electrode 6 ′, and discharges the target substance of the liquid sample in the container A to the container B. The negatively charged target substance contained in the liquid sample S is charged by charging the electrode 2 in the thin tube 1 and the electrode 6 in the container A in the negative state with the thin tube 1 attached to the liquid sample S. The substance flows into the capillary 1 due to its polarity. At this time, since a substance having no charge does not move, only a substance having the same polarity as the target substance is taken into the thin tube 1, and a substance having no polarity and a substance having a different polarity are excluded. Therefore, in the present invention, the sample can be purified simultaneously with the suction of the sample.
【０００８】目的物質を吸引した細管１は、図示しない
上下・左右移動機構により容器Ａから退避させて、容器
Ｂ内に挿入する（図２（ｂ）参照）。細管１内の電極２
の極性を反転させ、電極２をマイナスに、容器Ｂ内の電
極６’をプラスに荷電させる。目的物質はマイナスに荷
電しているので、容器Ｂ内にサンプルは吐出される。例
えば、ＤＮＡはマイナスに荷電しているので、本発明に
よれば、容易にＤＮＡのハンドリングを行うことができ
る。なお、以上の説明で吸引・吐出する液体サンプルの
量は、電極２、６（６’）に印加する電界強度及び電界
をかける時間の制御により行うことができる。The thin tube 1 having sucked the target substance is retracted from the container A by a vertical / left / right moving mechanism (not shown) and inserted into the container B (see FIG. 2B). Electrode 2 in capillary 1
, The electrode 2 is charged negative, and the electrode 6 ′ in the container B is charged positive. Since the target substance is negatively charged, the sample is discharged into the container B. For example, since DNA is negatively charged, DNA can be easily handled according to the present invention. In the above description, the amount of the liquid sample to be sucked / discharged can be controlled by controlling the electric field intensity applied to the electrodes 2 and 6 (6 ′) and the time for applying the electric field.
【０００９】本発明によれば、目的サンプルの吐出は、
溶液を吐出するのではないので、容器内で泡をかんだり
はしない。したがって、例えばマイクロチップ電気泳動
装置へのサンプル供給の際に問題となるサンプル導入の
際に発生する泡が電極を覆い、泳動が安定しないといっ
た問題の低減にも効果がある。According to the present invention, the discharge of the target sample is
It does not discharge the solution, so it does not chew bubbles in the container. Therefore, for example, bubbles generated when introducing a sample, which is a problem when supplying a sample to a microchip electrophoresis apparatus, cover the electrodes, which is also effective in reducing the problem of unstable electrophoresis.
【００１０】なお、本発明の液体移送器は上記構成に限
定されず、図３の構成でもよい。図３（a）は細管の代
わりに溶液またはゲルなどを含む多孔質素材を用いたも
ので、図中３１は多孔質素材を示し、ガラス管３３内に
挿入され、多孔質素材３１には電極３２が設置されてい
る。また、図３（ｂ）は細管の代わりに先端部をゲルで
直接構成したもので、ゲル３４はガラス管３３内に挿入
され、ゲル３４には電極３２が設置されている。多孔質
素材を使用した場合は細管とは異なり多彩な形状のヘッ
ドを作成することができ、直接ゲルで構成する場合は、
ディスポ的な用途に適している。[0010] The liquid transfer device of the present invention is not limited to the above-described structure, and may have the structure shown in FIG. FIG. 3 (a) uses a porous material containing a solution or gel instead of a thin tube. In the figure, reference numeral 31 denotes a porous material, which is inserted into a glass tube 33, and the porous material 31 has an electrode. 32 are installed. FIG. 3 (b) shows a structure in which the tip is directly formed of a gel instead of a thin tube. A gel 34 is inserted into a glass tube 33, and an electrode 32 is provided on the gel 34. When a porous material is used, heads of various shapes can be created unlike capillary tubes, and when directly composed of gel,
Suitable for disposable applications.
【００１１】[0011]
【発明の効果】本発明によれば、液体移送器の先端部の
微細化が可能であるので、微量サンプルを微小な容器内
でハンドリングを行う際に効果がある。また、電気泳動
によりサンプルを吸引するので、サンプル吸引時に同時
にサンプルの精製も行える。According to the present invention, the tip of the liquid transfer device can be miniaturized, which is effective in handling a small amount of sample in a small container. Further, since the sample is aspirated by electrophoresis, the sample can be purified at the same time as the sample is aspirated.
【図１】本発明の液体移送器の一実施例図FIG. 1 is a diagram showing one embodiment of a liquid transfer device of the present invention.
【図２】マイナス荷電物質を対象としたサンプラー使用
例を説明する図FIG. 2 is a diagram illustrating an example of using a sampler for a negatively charged substance.
【図３】本発明の液体移送器の変形実施例図FIG. 3 is a diagram showing a modified embodiment of the liquid transfer device of the present invention.
１：細管 ２、６、６’、３２：電極 ３：電源 ５、５’：切換えスイッチ 1: Capillary tube 2, 6, 6 ', 32: Electrode 3: Power supply 5, 5': Changeover switch
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） Ｇ０１Ｎ 27/447 Ｃ１２Ｍ 1/00 Ａ // Ｃ１２Ｍ 1/00 Ｇ０１Ｎ 27/26 ３０１Ｃ Ｆターム(参考） 2G052 AA28 AB16 AB20 AC11 AD06 AD26 AD46 BA24 CA03 CA04 CA08 CA22 CA23 CA24 CA28 DA03 DA06 DA09 ED04 ED06 GA22 HC03 HC07 HC21 HC38 JA01 JA05 JA11 JA13 4B029 AA09 AA23 BB20 HA05 4D054 FA10 FB01 4G068 AA01 AB11 AB15 AB30 AC17 AD50 ──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. 7 Identification symbol FI theme coat ゛ (reference) G01N 27/447 C12M 1/00 A // C12M 1/00 G01N 27/26 301C F-term (reference) 2G052 AA28 AB16 AB20 AC11 AD06 AD26 AD46 BA24 CA03 CA04 CA08 CA22 CA23 CA24 CA28 DA03 DA06 DA09 ED04 ED06 GA22 HC03 HC07 HC21 HC38 JA01 JA05 JA11 JA13 4B029 AA09 AA23 BB20 HA05 4D054 FA10 FB01 4G068 AA01 AB11 AB15 AB30 AC17
Claims (2)
引部材と、前記吸引部材内に収容される電極と、前記電
極の極性を切換える極性反転器とからなる液体移送器。1. A liquid transfer device comprising: a suction member having an opening for sucking a charged substance; an electrode housed in the suction member; and a polarity inverter for switching the polarity of the electrode.
該容器内に挿入する請求項１記載の液体移送器と、前記
容器内のサンプルの電荷を前記液体移送器内の極性と逆
の極性に荷電させる電界手段とを備えてなる液体吸引・
吐出装置。2. A container for containing or discharging a liquid sample,
2. A liquid suction device comprising: the liquid transfer device according to claim 1, which is inserted into the container; and electric field means for charging a charge of the sample in the container to a polarity opposite to the polarity in the liquid transfer device.
Discharge device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001141450A JP2002336678A (en) | 2001-05-11 | 2001-05-11 | Liquid transferring container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001141450A JP2002336678A (en) | 2001-05-11 | 2001-05-11 | Liquid transferring container |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2002336678A true JP2002336678A (en) | 2002-11-26 |
Family
ID=18987911
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001141450A Pending JP2002336678A (en) | 2001-05-11 | 2001-05-11 | Liquid transferring container |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2002336678A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004059295A1 (en) * | 2002-12-25 | 2004-07-15 | Cluster Technology Co., Ltd. | Method and device for discharging liquid specimen |
| JP2016218070A (en) * | 2015-05-18 | 2016-12-22 | 努 升島 | Electric field capture, free separation and molecular detection method of 1-cell or ultra-micro molecule |
-
2001
- 2001-05-11 JP JP2001141450A patent/JP2002336678A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004059295A1 (en) * | 2002-12-25 | 2004-07-15 | Cluster Technology Co., Ltd. | Method and device for discharging liquid specimen |
| JPWO2004059295A1 (en) * | 2002-12-25 | 2006-04-27 | クラスターテクノロジー株式会社 | Liquid sample discharge method and apparatus |
| JP4517123B2 (en) * | 2002-12-25 | 2010-08-04 | クラスターテクノロジー株式会社 | Liquid sample discharge method and apparatus |
| JP2016218070A (en) * | 2015-05-18 | 2016-12-22 | 努 升島 | Electric field capture, free separation and molecular detection method of 1-cell or ultra-micro molecule |
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