JP2001506135A - 変異ヒトα7アセチルコリン受容体サブユニットならびにその製造法および用途 - Google Patents
変異ヒトα7アセチルコリン受容体サブユニットならびにその製造法および用途Info
- Publication number
- JP2001506135A JP2001506135A JP52893398A JP52893398A JP2001506135A JP 2001506135 A JP2001506135 A JP 2001506135A JP 52893398 A JP52893398 A JP 52893398A JP 52893398 A JP52893398 A JP 52893398A JP 2001506135 A JP2001506135 A JP 2001506135A
- Authority
- JP
- Japan
- Prior art keywords
- human
- nachr
- subunit
- mutant
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Neurology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 野生型ヒトα7ニコチン性アセチルコリン受容体(nAChR)サブユニット の変異体をコードするポリヌクレオチドであって、野生型ヒトα7 nAChRサブユ ニットポリペプチドのバリン274の位置にアミノ酸置換を有するポリペプチドを コードすることを特徴とするポリヌクレオチド、およびその縮重変異体。 2. 該置換がバリン274からトレオニンへの置換である、請求項1に記載のポ リヌクレオチド。 3. 請求項1に記載のポリヌクレオチドを含んでなる宿主細胞。 4. 細菌細胞、哺乳類細胞、酵母細胞、両生類細胞およびヒトデ類細胞よりな る群から選ばれる、請求項3に記載の宿主細胞。 5. 該ポリヌクレオチドが宿主細胞内で発現されるように該ポリヌクレオチド の転写を指令する制御配列に機能しうる形で連結された請求項1に記載のポリヌ クレオチドを含んでなる発現ベクター。 6. 該変異ヒトα7 nAChRがヒトα5V274T nAChRである、請 求項5に記載の発現ベクター。 7. 請求項5または6に記載の発現ベクターを含んでなる宿主細胞。 8. 細菌細胞、哺乳類細胞、酵母細胞および両生類細胞よりなる群から選ばれ る、請求項7に記載の宿主細胞。 9. 野生型ヒトα7 nAChRポリペプチドのバリン274の位置にアミノ酸置換を 含んでなる、単離および精製された変異ヒトα7ニコチン性アセチルコリン受容 体(nAChR)サブユニット。 10. 該置換がバリン274からトレオニンへの置換である、請求項9に記載の変 異ヒトα7受容体。 11. ニコチン性アセチルコリン受容体(nAChR)活性をモジュレーションする 化合物を同定するための方法であって、 (a)野生型ヒトα7 nAChRポリペプチドのバリン274の位置にアミノ酸置換を 有する変異ヒトα7 nAChRポリペプチドを発現する細胞を準備し、 (b)試験化合物と該細胞とを混合し、 (c)(i)該変異α7受容体に対する又は該サブユニットを発現する細胞に対 する該試験化合物の効果、または(ii)該細胞または該受容体に対する該試験化 合物の結合のいずれかを測 定することを含んでなる方法。 12. 細胞保護性化合物を同定するための方法であって、 (a)野生型ヒトα7サブユニットポリペプチドのバリン274の位置にアミノ酸 置換を有する変異ヒトα7サブユニットポリペプチドまたはその断片を発現する 細胞を準備し、 (b)試験化合物と該細胞とを一緒にし、 (c)細胞傷害性の指標に関して該細胞または細胞機能をモニターすることを 含んでなる方法。 13. 試験サンプル中のヒト変異α7サブユニットの標的ポリヌクレオチドを検 出する方法であって、 (a)ヒト変異α7サブユニットの標的ポリヌクレオチドを少なくとも1つの ヒト変異α7サブユニット特異的ポリヌクレオチドプローブまたはその相補体と 接触させ、 (b)該試験サンプル中の標的ポリヌクレオチドとプローブとの複合体の存在 を検出することを含んでなる方法。 14. ヒト変異α7サブユニットの核酸に選択的にハイブリダイズしうる、ヒト 変異α7サブユニットに由来する精製されたポリヌクレオチドまたはその断片で あって、該ポリヌクレオチドの配列が配列番号1またはその一部を含み、該ポリ ヌクレオ チドが組換え技術により製造されることを特徴とするポリヌクレオチド。 15. ヒト変異α7サブユニットポリヌクレオチドにコードされるポリペプチド であって、該ポリペプチドの配列が配列番号2またはその一部を含み、該ポリペ プチドが組換えまたは合成技術により製造されることを特徴とするポリペプチド 。 16. 配列番号2またはその一部のアミノ酸配列を含むヒト変異α7サブユニッ トに特異的に結合するモノクローナル抗体。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/771,737 | 1996-12-20 | ||
US08/771,737 US6323000B2 (en) | 1996-12-20 | 1996-12-20 | Variant human α7 acetylcholine receptor subunit, and methods of production and uses thereof |
PCT/US1997/023405 WO1998028331A2 (en) | 1996-12-20 | 1997-12-22 | A variant human alpha-7 acetylcholine receptor subunit, and methods of production and use thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2001506135A true JP2001506135A (ja) | 2001-05-15 |
Family
ID=25092816
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP52893398A Pending JP2001506135A (ja) | 1996-12-20 | 1997-12-22 | 変異ヒトα7アセチルコリン受容体サブユニットならびにその製造法および用途 |
Country Status (5)
Country | Link |
---|---|
US (2) | US6323000B2 (ja) |
EP (1) | EP0946726A2 (ja) |
JP (1) | JP2001506135A (ja) |
CA (1) | CA2275180A1 (ja) |
WO (1) | WO1998028331A2 (ja) |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004081048A1 (ja) * | 2003-03-13 | 2004-09-23 | Chugai Seiyaku Kabushiki Kaisha | 変異受容体に対するアゴニスト活性を有するリガンド |
US7691588B2 (en) | 2003-03-13 | 2010-04-06 | Chugai Seiyaku Kabushiki Kaisha | Ligand having agonistic activity to mutated receptor |
US7696325B2 (en) | 1999-03-10 | 2010-04-13 | Chugai Seiyaku Kabushiki Kaisha | Polypeptide inducing apoptosis |
US7993642B2 (en) | 2003-12-12 | 2011-08-09 | Chugai Seiyaku Kabushiki Kaisha | Anti-MPL antibodies |
US8034903B2 (en) | 2000-10-20 | 2011-10-11 | Chugai Seiyaku Kabushiki Kaisha | Degraded TPO agonist antibody |
JP2012504966A (ja) * | 2008-10-09 | 2012-03-01 | ハワード ヒューズ メディカル インスティチュート | 新規なキメラリガンド開口型イオンチャネルおよびその使用方法 |
US8158385B2 (en) | 2002-10-11 | 2012-04-17 | Chugai Seiyaku Kabushiki Kaisha | Cell death-inducing agent |
US8945543B2 (en) | 2005-06-10 | 2015-02-03 | Chugai Seiyaku Kabushiki Kaisha | Stabilizer for protein preparation comprising meglumine and use thereof |
US9241994B2 (en) | 2005-06-10 | 2016-01-26 | Chugai Seiyaku Kabushiki Kaisha | Pharmaceutical compositions containing sc(Fv)2 |
US9493569B2 (en) | 2005-03-31 | 2016-11-15 | Chugai Seiyaku Kabushiki Kaisha | Structural isomers of sc(Fv)2 |
US9670269B2 (en) | 2006-03-31 | 2017-06-06 | Chugai Seiyaku Kabushiki Kaisha | Methods of modifying antibodies for purification of bispecific antibodies |
US10011858B2 (en) | 2005-03-31 | 2018-07-03 | Chugai Seiyaku Kabushiki Kaisha | Methods for producing polypeptides by regulating polypeptide association |
US11124576B2 (en) | 2013-09-27 | 2021-09-21 | Chungai Seiyaku Kabushiki Kaisha | Method for producing polypeptide heteromultimer |
US11649262B2 (en) | 2015-12-28 | 2023-05-16 | Chugai Seiyaku Kabushiki Kaisha | Method for promoting efficiency of purification of Fc region-containing polypeptide |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6693172B1 (en) | 1999-05-27 | 2004-02-17 | Pharmacia And Upjohn Company | Double mutant alpha-7 nicotinic acetylcholine receptor |
EP1180142A2 (en) * | 1999-05-27 | 2002-02-20 | PHARMACIA & UPJOHN COMPANY | Methods and compositions for measuring ion channel conductance |
US20030092613A1 (en) * | 2000-08-14 | 2003-05-15 | Lee Daniel H. S. | Alpha7 nicotinic receptor peptides as ligands for beta amyloid peptides |
EP2351838A1 (en) * | 2000-10-20 | 2011-08-03 | Chugai Seiyaku Kabushiki Kaisha | Crosslinking agonistic antibodies |
US7358057B2 (en) * | 2002-05-09 | 2008-04-15 | Memory Pharmaceuticals Corporation | QM-7 and QT-6 cells transfected with mutant cell surface expressed channel receptors and assays using the transfected cells |
US7238715B2 (en) * | 2002-12-06 | 2007-07-03 | The Feinstein Institute For Medical Research | Treatment of pancreatitis using alpha 7 receptor-binding cholinergic agonists |
ES2462545T3 (es) * | 2002-12-06 | 2014-05-23 | The Feinstein Institute For Medical Research | Un procedimiento para la determinación de un agonista colinérgico selectivo para un receptor alfa7 nicotínico |
EP1592710A2 (en) * | 2003-02-14 | 2005-11-09 | Memory Pharmaceutical Corporation | Macaque alpha-7 nicotinic acetylcholine receptor and methods of use thereof |
EP1609803A4 (en) * | 2003-03-31 | 2006-05-24 | Chugai Pharmaceutical Co Ltd | MODIFIED ANTIBODY AGAINST CD22 AND ITS USE |
US8597911B2 (en) * | 2003-06-11 | 2013-12-03 | Chugai Seiyaku Kabushiki Kaisha | Process for producing antibodies |
EP1712565A4 (en) * | 2003-12-12 | 2009-03-11 | Chugai Pharmaceutical Co Ltd | AGENTS INDUCING CELL DEATH |
WO2005056602A1 (ja) * | 2003-12-12 | 2005-06-23 | Chugai Seiyaku Kabushiki Kaisha | アゴニスト活性を有する改変抗体のスクリーニング方法 |
US20050170360A1 (en) * | 2004-01-30 | 2005-08-04 | Papke Roger L. | Variant neuronal nicotinic alpha-7 receptor and methods of use |
WO2005100560A1 (ja) * | 2004-04-09 | 2005-10-27 | Chugai Seiyaku Kabushiki Kaisha | 細胞死誘導剤 |
WO2006123724A1 (ja) * | 2005-05-18 | 2006-11-23 | The University Of Tokushima | 抗hla抗体を利用した新規医薬品 |
TW200718780A (en) * | 2005-06-10 | 2007-05-16 | Chugai Pharmaceutical Co Ltd | Sc(Fv)2 site-directed mutant |
US7879913B2 (en) * | 2005-06-28 | 2011-02-01 | Catholic Healthcare West | Iptakalim hydrochloride for decreasing nicotine use |
US8316104B2 (en) | 2005-11-15 | 2012-11-20 | California Institute Of Technology | Method and apparatus for collaborative system |
RU2009100930A (ru) * | 2006-06-14 | 2010-07-20 | Чугаи Сейяку Кабусики Кайся (Jp) | Лекарственные средства для стимуляции роста гемопоэтических стволовых клеток |
KR20090038896A (ko) * | 2006-07-13 | 2009-04-21 | 추가이 세이야쿠 가부시키가이샤 | 세포사 유도제 |
CL2008000719A1 (es) * | 2007-03-12 | 2008-09-05 | Univ Tokushima Chugai Seiyaku | Agente terapeutico para cancer resistente a agentes quimioterapeuticos que comprende un anticuerpo que reconoce hla de clase i como ingrediente activo; composicion farmaceutica que comprende dicho anticuerpo; y metodo para tratar cancer resistente a |
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CA2071879C (en) * | 1989-10-27 | 2007-05-15 | Stephen F. Heinemann | Glutamate receptor compositions and methods |
GB2286397B (en) * | 1993-03-08 | 1997-09-10 | Salk Inst Biotech Ind | Human neuronal nicotinic acetylcholine receptor compositions and methods of employing same |
-
1996
- 1996-12-20 US US08/771,737 patent/US6323000B2/en not_active Expired - Fee Related
-
1997
- 1997-12-22 CA CA002275180A patent/CA2275180A1/en not_active Abandoned
- 1997-12-22 JP JP52893398A patent/JP2001506135A/ja active Pending
- 1997-12-22 EP EP97954155A patent/EP0946726A2/en not_active Withdrawn
- 1997-12-22 WO PCT/US1997/023405 patent/WO1998028331A2/en active Application Filing
-
2001
- 2001-09-18 US US09/954,936 patent/US6683157B2/en not_active Expired - Fee Related
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7696325B2 (en) | 1999-03-10 | 2010-04-13 | Chugai Seiyaku Kabushiki Kaisha | Polypeptide inducing apoptosis |
US8586039B2 (en) | 2000-10-20 | 2013-11-19 | Chugai Seiyaku Kabushiki Kaisha | Degraded TPO agonist antibody |
US8034903B2 (en) | 2000-10-20 | 2011-10-11 | Chugai Seiyaku Kabushiki Kaisha | Degraded TPO agonist antibody |
US8158385B2 (en) | 2002-10-11 | 2012-04-17 | Chugai Seiyaku Kabushiki Kaisha | Cell death-inducing agent |
JPWO2004081048A1 (ja) * | 2003-03-13 | 2006-08-31 | 中外製薬株式会社 | 変異受容体に対するアゴニスト活性を有するリガンド |
US7691588B2 (en) | 2003-03-13 | 2010-04-06 | Chugai Seiyaku Kabushiki Kaisha | Ligand having agonistic activity to mutated receptor |
JP4739954B2 (ja) * | 2003-03-13 | 2011-08-03 | 中外製薬株式会社 | 変異受容体に対するアゴニスト活性を有するリガンド |
WO2004081048A1 (ja) * | 2003-03-13 | 2004-09-23 | Chugai Seiyaku Kabushiki Kaisha | 変異受容体に対するアゴニスト活性を有するリガンド |
US7993642B2 (en) | 2003-12-12 | 2011-08-09 | Chugai Seiyaku Kabushiki Kaisha | Anti-MPL antibodies |
US8008073B2 (en) | 2003-12-12 | 2011-08-30 | Chugai Seiyaku Kabushiki Kaisha | Anti-Mpl antibodies |
US9493569B2 (en) | 2005-03-31 | 2016-11-15 | Chugai Seiyaku Kabushiki Kaisha | Structural isomers of sc(Fv)2 |
US10011858B2 (en) | 2005-03-31 | 2018-07-03 | Chugai Seiyaku Kabushiki Kaisha | Methods for producing polypeptides by regulating polypeptide association |
US11168344B2 (en) | 2005-03-31 | 2021-11-09 | Chugai Seiyaku Kabushiki Kaisha | Methods for producing polypeptides by regulating polypeptide association |
US8945543B2 (en) | 2005-06-10 | 2015-02-03 | Chugai Seiyaku Kabushiki Kaisha | Stabilizer for protein preparation comprising meglumine and use thereof |
US9241994B2 (en) | 2005-06-10 | 2016-01-26 | Chugai Seiyaku Kabushiki Kaisha | Pharmaceutical compositions containing sc(Fv)2 |
US9777066B2 (en) | 2005-06-10 | 2017-10-03 | Chugai Seiyaku Kabushiki Kaisha | Pharmaceutical compositions containing sc(Fv)2 |
US9670269B2 (en) | 2006-03-31 | 2017-06-06 | Chugai Seiyaku Kabushiki Kaisha | Methods of modifying antibodies for purification of bispecific antibodies |
US10934344B2 (en) | 2006-03-31 | 2021-03-02 | Chugai Seiyaku Kabushiki Kaisha | Methods of modifying antibodies for purification of bispecific antibodies |
JP2012504966A (ja) * | 2008-10-09 | 2012-03-01 | ハワード ヒューズ メディカル インスティチュート | 新規なキメラリガンド開口型イオンチャネルおよびその使用方法 |
US11124576B2 (en) | 2013-09-27 | 2021-09-21 | Chungai Seiyaku Kabushiki Kaisha | Method for producing polypeptide heteromultimer |
US11649262B2 (en) | 2015-12-28 | 2023-05-16 | Chugai Seiyaku Kabushiki Kaisha | Method for promoting efficiency of purification of Fc region-containing polypeptide |
Also Published As
Publication number | Publication date |
---|---|
WO1998028331A3 (en) | 1998-11-05 |
EP0946726A2 (en) | 1999-10-06 |
WO1998028331A2 (en) | 1998-07-02 |
US20030073161A1 (en) | 2003-04-17 |
US6323000B2 (en) | 2001-11-27 |
US6683157B2 (en) | 2004-01-27 |
CA2275180A1 (en) | 1998-07-02 |
US20010006796A1 (en) | 2001-07-05 |
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