JP2001275689A5 - - Google Patents
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- JP2001275689A5 JP2001275689A5 JP2001018359A JP2001018359A JP2001275689A5 JP 2001275689 A5 JP2001275689 A5 JP 2001275689A5 JP 2001018359 A JP2001018359 A JP 2001018359A JP 2001018359 A JP2001018359 A JP 2001018359A JP 2001275689 A5 JP2001275689 A5 JP 2001275689A5
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- protein
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Description
【特許請求の範囲】
【請求項1】 下記(A)又は(B)に示すタンパク質。
(A)配列表の配列番号2に記載のアミノ酸配列を有するタンパク質。
(B)配列表の配列番号2に記載のアミノ酸配列において、1若しくは数個のアミノ酸の置換、欠失、挿入、付加、又は逆位を含むアミノ酸配列からなり、かつ、ホスホセリンホスファターゼ活性を有するタンパク質。
【請求項2】 下記(A)又は(B)に示すタンパク質をコードするDNA。
(A)配列表の配列番号2に記載のアミノ酸配列を有するタンパク質。
(B)配列表の配列番号2に記載のアミノ酸配列において、1若しくは数個のアミノ酸の置換、欠失、挿入、付加、又は逆位を含むアミノ酸配列からなり、かつ、ホスホセリンホスファターゼ活性を有するタンパク質。
【請求項3】 配列表の配列番号2に記載のアミノ酸配列を有するタンパク質をコードするDNAとストリンジェントな条件でハイブリダイズし、かつ、ホスホセリンホスファターゼ活性を有するタンパク質をコードするDNA。
【請求項4】 前記ストリンジェントな条件が、1×SSC及び0.1%SDSに相当する塩濃度で60℃で洗浄が行われる条件である請求項3記載のDNA。
【請求項5】 下記(a)又は(b)に示すDNAである請求項2記載のDNA。
(a)配列表の配列番号1に記載の塩基配列のうち、塩基番号210〜1547からなる塩基配列を含むDNA。
(b)配列表の配列番号1に記載の塩基配列のうち、塩基番号210〜1547からなる塩基配列又はその一部を有するプローブとストリンジェントな条件下でハイブリダイズし、かつ、ホスホセリンホスファターゼ活性を有するタンパク質をコードするDNA。
【請求項6】 前記ストリンジェントな条件が、1×SSC及び0.1%SDSに相当する塩濃度で60℃で洗浄が行われる条件である請求項5記載のDNA。
【請求項7】 請求項1〜6のいずれか一項に記載のDNAを含むベクター。
【請求項8】 請求項1〜6のいずれか一項に記載のDNAによってコードされるホスホセリンホスファターゼの活性が上昇した細菌。
【請求項9】 請求項8記載の細菌を培地に培養し、該培地中にL−セリンを生成蓄積させ、該培地からL−セリンを採取することを特徴とするL−セリンの製造法。
[Claims]
1. A protein represented by the following (A) or (B):
(A) a protein having the amino acid sequence of SEQ ID NO: 2 in the sequence listing;
(B) the amino acid sequence represented by SEQ ID NO: 2 in the sequence listing, comprising an amino acid sequence containing substitution, deletion, insertion, addition, or inversion of one or several amino acids, and having a phosphoserine phosphatase activity; protein.
2. A DNA encoding a protein represented by the following (A) or (B):
(A) a protein having the amino acid sequence of SEQ ID NO: 2 in the sequence listing;
(B) the amino acid sequence represented by SEQ ID NO: 2 in the sequence listing, comprising an amino acid sequence containing substitution, deletion, insertion, addition, or inversion of one or several amino acids, and having a phosphoserine phosphatase activity; protein.
3. A DNA that hybridizes with a DNA encoding a protein having the amino acid sequence of SEQ ID NO: 2 under stringent conditions and encodes a protein having phosphoserine phosphatase activity.
4. The DNA according to claim 3, wherein the stringent condition is a condition in which washing is performed at 60 ° C. at a salt concentration corresponding to 1 × SSC and 0.1% SDS.
5. The DNA according to claim 2, which is a DNA represented by the following (a) or (b):
(A) DNA comprising a base sequence consisting of base numbers 210 to 1547 in the base sequence described in SEQ ID NO: 1 in the sequence listing.
(B) Sequence number of sequence listingIn oneA DNA that hybridizes with a probe having a base sequence consisting of base numbers 210 to 1547 or a part thereof in the described base sequence under stringent conditions and encodes a protein having phosphoserine phosphatase activity.
6. The DNA according to claim 5, wherein the stringent condition is a condition in which washing is performed at 60 ° C. at a salt concentration corresponding to 1 × SSC and 0.1% SDS.
7. ClaimA vector comprising the DNA according to any one of claims 1 to 6.
A bacterium having an increased activity of phosphoserine phosphatase encoded by the DNA according to any one of claims 1 to 6.
9. A method for producing L-serine, comprising culturing the bacterium according to claim 8 in a medium, producing and accumulating L-serine in the medium, and collecting L-serine from the medium.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2001018359A JP4655374B2 (en) | 2000-01-27 | 2001-01-26 | The phosphoserine phosphatase gene of coryneform bacteria |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000023341 | 2000-01-27 | ||
JP2000-23341 | 2000-01-27 | ||
JP2001018359A JP4655374B2 (en) | 2000-01-27 | 2001-01-26 | The phosphoserine phosphatase gene of coryneform bacteria |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2001275689A JP2001275689A (en) | 2001-10-09 |
JP2001275689A5 true JP2001275689A5 (en) | 2007-07-12 |
JP4655374B2 JP4655374B2 (en) | 2011-03-23 |
Family
ID=26584586
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2001018359A Expired - Lifetime JP4655374B2 (en) | 2000-01-27 | 2001-01-26 | The phosphoserine phosphatase gene of coryneform bacteria |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP4655374B2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011067095A (en) | 2008-01-10 | 2011-04-07 | Ajinomoto Co Inc | Method for producing target substance by fermentation process |
WO2015050234A1 (en) | 2013-10-02 | 2015-04-09 | 味の素株式会社 | Ammonia control apparatus and ammonia control method |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SK18872001A3 (en) * | 1999-06-25 | 2002-12-03 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding proteins involved in carbon metabolism and energy production |
KR20070087035A (en) * | 1999-06-25 | 2007-08-27 | 바스프 악티엔게젤샤프트 | Corynebacterium glutamicum genes encoding metabolic pathway proteins |
DE10044831A1 (en) * | 2000-03-01 | 2002-04-04 | Forschungszentrum Juelich Gmbh | Improved process for the microbial production of L-serine and a suitable genetically modified microorganism |
-
2001
- 2001-01-26 JP JP2001018359A patent/JP4655374B2/en not_active Expired - Lifetime
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