JP2001141695A5 - A method for measuring the concentration of dopamine contained in a test sample containing dopamine and ascorbic acid, and a modified electrode used in this method. - Google Patents
A method for measuring the concentration of dopamine contained in a test sample containing dopamine and ascorbic acid, and a modified electrode used in this method. Download PDFInfo
- Publication number
- JP2001141695A5 JP2001141695A5 JP1999324014A JP32401499A JP2001141695A5 JP 2001141695 A5 JP2001141695 A5 JP 2001141695A5 JP 1999324014 A JP1999324014 A JP 1999324014A JP 32401499 A JP32401499 A JP 32401499A JP 2001141695 A5 JP2001141695 A5 JP 2001141695A5
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- Japan
- Prior art keywords
- dopamine
- ascorbic acid
- test sample
- modified electrode
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 title description 40
- 229960003638 dopamine Drugs 0.000 title description 20
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Vitamin C Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title description 11
- 229960005070 ascorbic acid Drugs 0.000 title description 11
- 235000010323 ascorbic acid Nutrition 0.000 title description 11
- 239000011668 ascorbic acid Substances 0.000 title description 11
- 239000000758 substrate Substances 0.000 description 12
- 239000000126 substance Substances 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 6
- JUJWROOIHBZHMG-UHFFFAOYSA-O Pyridinium Chemical group C1=CC=[NH+]C=C1 JUJWROOIHBZHMG-UHFFFAOYSA-O 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O ammonium group Chemical group [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- 125000000129 anionic group Chemical group 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 125000001841 imino group Chemical group [H]N=* 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- JUJWROOIHBZHMG-UHFFFAOYSA-N pyridine Chemical group C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 3
- APQPRKLAWCIJEK-UHFFFAOYSA-N 51-85-4 Chemical compound NCCSSCCN APQPRKLAWCIJEK-UHFFFAOYSA-N 0.000 description 2
- 210000001124 Body Fluids Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000005868 electrolysis reaction Methods 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 210000004369 Blood Anatomy 0.000 description 1
- 210000004556 Brain Anatomy 0.000 description 1
- 210000003296 Saliva Anatomy 0.000 description 1
- 210000004243 Sweat Anatomy 0.000 description 1
- 210000001138 Tears Anatomy 0.000 description 1
- 210000002700 Urine Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 150000001767 cationic compounds Chemical class 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
Description
【特許請求の範囲】
【請求項１】 ドーパミンおよびアスコルビン酸を含む被検試料に含まれるドーパミンの濃度を測定する方法であって、２つの電解室を両側に有する電解セルのうち、一方の電解室に作用電極、参照電極、および修飾電極が入れられており、他方の電解室に対極が入れられており、ここで、前記修飾電極は、導電性の基板を有し、前記基板の表面が、ピリジン基またはピリジニウム基を有する化合物、あるいはイミノ基、アミノ基、またはアンモニウム基を有する化合物によって修飾されており、前記電解セルに前記被検試料を入れた後、前記作用電極の電位を前記参照電極に対して０Ｖ付近からプラス方向に掃印することによって得られる電流−電圧曲線において＋０．２Ｖ付近に現れる電流ピークを前記ドーパミンの濃度として測定する方法。
【請求項２】 前記基板の表面が、チオアルキルアミンによって修飾されている、請求項１に記載の方法。
【請求項３】 前記チオアルキルアミンが、（化１）で示されるジチオビスアルキルアミンである、請求項２に記載の方法。
【化１】
【化２】
【請求項６】 ドーパミンおよびアスコルビン酸を含む被検試料に含まれるドーパミンの濃度を測定するために用いられる修飾電極であって、
前記修飾電極は、導電性の基板を有し、前記基板の表面が、ピリジン基またはピリジニウム基を有する化合物、あるいはイミノ基、アミノ基、またはアンモニウム基を有する化合物によって修飾されている、修飾電極。
【請求項７】 前記基板の表面が、チオアルキルアミンによって修飾されている、請求項６に記載の修飾電極。
【請求項８】 前記チオアルキルアミンが、（化３）で示されるジチオビスアルキルアミンである、請求項７に記載の修飾電極。
【化３】
【化４】
[Claim 1] A method for measuring the concentration of dopamine contained in a test sample containing dopamine and ascorbic acid, in which an action electrode, a reference electrode, and a modified electrode are placed in one of the electrolytic cells having two electrolytic chambers on both sides. The modified electrode has a conductive substrate, and the surface of the substrate has a pyridine group or a pyridinium group, or a compound having a counter electrode in the other electrolytic chamber. It is modified with a compound having an imino group, an amino group, or an ammonium group, and after the test sample is placed in the electrolytic cell, the potential of the working electrode is swept positively from around 0 V with respect to the reference electrode. A method of measuring the current peak appearing near +0.2 V in the current-voltage curve obtained by marking as the concentration of the dopamine...
2. The method of claim 1, wherein the surface of the substrate is modified with a thioalkylamine...
3. SaidThe thioalkylamine is the dithiobisalkylamine represented by (Chemical formula 1).,The second aspectMethod..
[Chemical 1]
[Chemical 2]
6. A modified electrode used to measure the concentration of dopamine contained in a test sample containing dopamine and ascorbic acid.
The modified electrode is a modified electrode having a conductive substrate whose surface is modified with a compound having a pyridine group or a pyridinium group, or a compound having an imino group, an amino group, or an ammonium group.
7. The modified electrode according to claim 6, wherein the surface of the substrate is modified with a thioalkylamine.
8. The modified electrode according to claim 7, wherein the thioalkylamine is the dithiobisalkylamine represented by (Chemical Formula 3).
[Chemical 3]
[Chemical 4]
【０００１】
【発明の属する技術分野】
本発明は、ドーパミンおよびアスコルビン酸を含む被検試料、特に、ヒトに代表される生物の血液、尿、汗、唾液、涙液、分泌液等の体液中に含まれるドーパミンおよびアスコルビン酸を含む被検試料に含まれるドーパミンの濃度を測定する方法、およびこの方法に用いられる修飾電極に関する。
[0001]
[Technical field to which the invention belongs]
The present invention relates to a test sample containing dopamine and ascorbic acid, particularly a subject containing dopamine and ascorbic acid contained in body fluids such as blood, urine, sweat, saliva, tears, and secretions of organisms typified by humans. The present invention relates to a method for measuring the concentration of dopamine contained in a test sample, and a modified electrode used in this method .
このような課題を取り除くために、アダムス（Ａｄａｍｓ）らは、アニオン性ポリマー膜で被覆した金電極を用いることを提案している（Ｂｒａｉｎ Ｒｅｓ．誌第３４巻（１９８５年）第１５１頁参照）。この場合、（化３）で示される、電解質中でプラスに荷電したドーパミンがアニオン性ポリマー膜を被覆した電極に静電的に引き付けられるので、ドーパミンの酸化還元電位をアスコルビン酸に比べて十分マイナスにすることができる。そのためアニオン性ポリマー膜修飾電極の電位を、ドーパミンが酸化され、かつアスコルビン酸が酸化されない電位に固定すると、ドーパミンを選択的に酸化することができ、アスコルビン酸の存在下でのドーパミンの選択的な検出が可能である。その他、特開平８−２１８１９号公報も先行技術文献として挙げることができる。 To eliminate this problem, Adams et al. Have proposed using a gold electrode coated with an anionic polymer film (see Brain Res., Vol. 34 (1985), p. 151). .. In this case, the positively charged dopamine in the electrolyte shown in (Chemical Formula 3) is electrostatically attracted to the electrode coated with the anionic polymer film, so that the redox potential of dopamine is sufficiently negative as compared with ascorbic acid. Can be. Therefore, by fixing the potential of the anionic polymer film-modified electrode to a potential at which dopamine is oxidized and ascorbic acid is not oxidized, dopamine can be selectively oxidized, and dopamine is selectively selected in the presence of ascorbic acid. It can be detected. In addition, Japanese Patent Application Laid-Open No. 8-21819 can also be mentioned as a prior art document.
本発明は、前記従来の問題に鑑みてなされたものであり、その目的は、体液のようにアスコルビン酸を含む被検試料であっても、被検試料中のドーパミンを速い応答速度で容易に定量することのできる方法およびその方法に用いられる修飾電極を提供することである。 The present invention has been made in view of the above-mentioned conventional problems, and an object of the present invention is to easily obtain dopamine in a test sample at a high response speed even in a test sample containing ascorbic acid such as a body fluid. It is to provide a method which can be quantified and a modified electrode used in the method.
【０００９】
【課題を解決するための手段】
前記目的を達成する本発明は、ドーパミンおよびアスコルビン酸を含む被検試料に含まれるドーパミンの濃度を測定する方法であって、２つの電解室を両側に有する電解セルのうち、一方の電解室に作用電極、参照電極、および修飾電極が入れられており、他方の電解室に対極が入れられており、ここで、前記修飾電極は、導電性の基板を有し、前記基板の表面が、ピリジン基またはピリジニウム基を有する化合物、あるいはイミノ基、アミノ基、またはアンモニウム基を有する化合物によって修飾されており、前記電解セルに前記被検試料を入れた後、前記作用電極の電位を前記参照電極に対して０Ｖ付近からプラス方向に掃印することによって得られる電流−電圧曲線において＋０．２Ｖ付近に現れる電流ピークを前記ドーパミンの濃度として測定する。
0009
[Means for solving problems]
The present invention that to achieve the above object is achieved by a method for measuring the concentration of dopamine contained in a test sample containing dopamine and ascorbic acid, among the electrolysis cell having two electrolyte chambers on either side, one of the electrolysis chamber A working electrode, a reference electrode, and a modified electrode are placed in the electrode, and a counter electrode is placed in the other electrolytic chamber. Here, the modified electrode has a conductive substrate, and the surface of the substrate has a surface. It is modified with a compound having a pyridine group or a pyridinium group, or a compound having an imino group, an amino group, or an ammonium group, and after the test sample is placed in the electrolytic cell, the potential of the working electrode is set to the reference electrode. The current peak appearing near + 0.2 V in the current-voltage curve obtained by sweeping from the vicinity of 0 V in the positive direction is measured as the concentration of the dopamine.
前記基板の表面が、チオアルキルアミンによって修飾されていることが好ましい。 It is preferable that the surface of the substrate is modified with a thioalkylamine.
また本発明は、前記の方法に用いられる修飾電極に関する。 The present invention also relates to a modified electrode used in the above method .
【００１９】
【発明の実施の形態】
本発明は、導電性の基板を有し、前記基板表面をカチオン性化合物で化学修飾した修飾電極を、ドーパミンおよびアスコルビン酸検出用の修飾電極として用いる。
0019
BEST MODE FOR CARRYING OUT THE INVENTION
In the present invention, a modified electrode having a conductive substrate and the surface of the substrate chemically modified with a cationic compound is used as a modified electrode for detecting dopamine and ascorbic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP32401499A JP4366791B2 (en) | 1999-11-15 | 1999-11-15 | Modified electrode used to measure the concentration of dopamine in test samples containing dopamine and ascorbic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP32401499A JP4366791B2 (en) | 1999-11-15 | 1999-11-15 | Modified electrode used to measure the concentration of dopamine in test samples containing dopamine and ascorbic acid |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2001141695A JP2001141695A (en) | 2001-05-25 |
| JP2001141695A5 true JP2001141695A5 (en) | 2006-08-10 |
| JP4366791B2 JP4366791B2 (en) | 2009-11-18 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP32401499A Expired - Lifetime JP4366791B2 (en) | 1999-11-15 | 1999-11-15 | Modified electrode used to measure the concentration of dopamine in test samples containing dopamine and ascorbic acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4366791B2 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4553168B2 (en) * | 2000-03-17 | 2010-09-29 | 国立大学法人東京工業大学 | Modified electrode for detection of 3,4-dihydroxyphenylacetic acid and / or homovanillic acid and detection method thereof |
| CN100343660C (en) * | 2005-09-28 | 2007-10-17 | 浙江大学 | Minisize vitamin C sensor and making method thereof |
| JP5366432B2 (en) * | 2008-05-09 | 2013-12-11 | 株式会社船井電機新応用技術研究所 | Gas detection system |
| CN101576530B (en) * | 2009-05-21 | 2012-02-08 | 北京化工大学 | Method for measuring dopamine by utilizing graphite nano-sheet/Nafion composite film to modify electrode |
| CN101726531B (en) * | 2009-12-16 | 2012-07-04 | 厦门大学 | Electrochemical detection method of dopamine in body fluid |
| WO2011093039A1 (en) * | 2010-01-27 | 2011-08-04 | 株式会社クラレ | Raw milk inspection method and raw milk inspection device |
| JP6317927B2 (en) * | 2012-01-09 | 2018-04-25 | ムー・メディカル・デバイスズ・エルエルシーＭｏｅ Ｍｅｄｉｃａｌ Ｄｅｖｉｃｅｓ Ｌｌｃ | Plasma assisted skin treatment |
| CN109444241A (en) * | 2018-12-25 | 2019-03-08 | 湖南科技大学 | A kind of dopamine detection method, detecting electrode and preparation method, electrochemical sensor |
| JP7346680B2 (en) | 2021-11-26 | 2023-09-19 | 株式会社ポケモン | Game program, method, information processing device |
| CN114324530B (en) * | 2022-01-07 | 2024-03-22 | 辽宁大学 | Nano cadmium sulfide modified electrode and method for synchronously measuring dopamine and ascorbic acid |
-
1999
- 1999-11-15 JP JP32401499A patent/JP4366791B2/en not_active Expired - Lifetime
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