JP2000146825A - Feeble emission measuring device - Google Patents
Feeble emission measuring deviceInfo
- Publication number
- JP2000146825A JP2000146825A JP10314225A JP31422598A JP2000146825A JP 2000146825 A JP2000146825 A JP 2000146825A JP 10314225 A JP10314225 A JP 10314225A JP 31422598 A JP31422598 A JP 31422598A JP 2000146825 A JP2000146825 A JP 2000146825A
- Authority
- JP
- Japan
- Prior art keywords
- light
- test tube
- light emission
- measurement
- detection unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は生物発光や化学発光
又は蛍光等微弱な発光を測定する装置に関し、特に、光
電子増倍管を使用した微弱発光計測装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a device for measuring weak light emission such as bioluminescence, chemiluminescence or fluorescence, and more particularly to a device for measuring weak light emission using a photomultiplier tube.
【０００２】[0002]
【従来の技術】生物発光や化学発光により生じる微弱な
発光を計測することにより、過酸化水素の定量、生物が
生活するために必要な代謝活性物質であるＡＴＰ（Aden
osinetriphosphate）の定量、酵素の定量などの様々な
物質の定量を行うことができる。例えば、測定対象物と
測定に必要な試薬等を添加し、発光反応を起こさせその
発光量を測定することで、間接的にＡＴＰ濃度等を計測
する。2. Description of the Related Art By measuring weak luminescence caused by bioluminescence or chemiluminescence, hydrogen peroxide can be quantified and ATP (Aden) which is a metabolic active substance necessary for living organisms to live.
Quantification of various substances such as quantification of osine triphosphate) and enzyme. For example, an ATP concentration or the like is indirectly measured by adding an object to be measured and a reagent required for the measurement, causing a luminescence reaction, and measuring the amount of luminescence.
【０００３】図６はこのような微弱な発光を測定するた
めの従来の計測装置で、その（Ａ）図は上面図、（Ｂ）
図は（Ａ）図のＡ−Ａ断面矢視図、（Ｃ）図はＢ−Ｂ断
面矢視図を示している。FIG. 6 shows a conventional measuring device for measuring such weak light emission. FIG. 6 (A) is a top view, and FIG.
The figure is a sectional view taken along the line AA in FIG. 7A, and the figure (C) is a sectional view taken along the line BB in FIG.
【０００４】これらの図において、３０は発光検出部、
４０は計測部を示し、発光検出部３０は暗室を形成する
暗箱３１と、暗箱３１内に挿入した試験管３４を支持す
る試験管ホルダ３２と、暗箱内で試験管内の発光を検出
する光電子増倍管３３とから成り、光電子増倍管３３は
受光部３３ａを有し、受光部３３ａを暗箱内に向けて暗
箱３１の外部に取り付けられている。光電子増倍管３３
は、受光部３３ａに強い光が入射されると計測不能とな
るため、試験管３４を暗箱３１内に挿出入するときに受
光部に外部からの光が入射しないようにする目的で、受
光部３３ａを常時遮蔽し、測定時にのみ開く第１のシャ
ッタ３５と、暗箱３１の上部に設けられた試験管挿入口
３７を常時閉じ、試験管３４を暗箱３１内に出し入れす
るときに開く第２のシャッタ３６を有する。In these figures, reference numeral 30 denotes a light emission detecting section,
Reference numeral 40 denotes a measuring unit, and a light emission detecting unit 30 includes a dark box 31 forming a dark room, a test tube holder 32 supporting a test tube 34 inserted in the dark box 31, and a photoelectron detector for detecting light emission in a test tube in the dark box. The photomultiplier tube 33 has a light receiving portion 33a, and is mounted outside the dark box 31 with the light receiving portion 33a facing the inside of the dark box. Photomultiplier tube 33
In order to prevent external light from entering the light receiving unit when the test tube 34 is inserted into and out of the dark box 31, the light receiving unit A first shutter 35 which is always shielded and opened only at the time of measurement, and a second shutter 37 which is always closed when the test tube insertion port 37 provided at the upper part of the dark box 31 is opened and the test tube 34 is opened and closed in the dark box 31. It has a shutter 36.
【０００５】発光体の光量の測定は、試験管３４に被測
定物（発光体）を入れ、第２のシャッタ３６を開け、挿
入口３７から試験管３４を入れて試験管ホルダ３２に固
定する。このとき、第１のシャッタ３５は閉じた状態と
なっている。In order to measure the light quantity of the luminous body, an object to be measured (luminous body) is put into a test tube 34, a second shutter 36 is opened, the test tube 34 is inserted through an insertion port 37, and the test tube holder 32 is fixed. . At this time, the first shutter 35 is in a closed state.
【０００６】試験管３４を試験管ホルダ３２に固定した
後、第２のシャッタ３６を閉じる。完全に閉じた後、第
１のシャッタ３５を開いて試験管内の微弱な光を光電子
増倍管３３の受光部３３ａで受光し、受光した光量をそ
れに比例した電気信号（増幅電流）を出力して計測部４
０に入力する。After fixing the test tube 34 to the test tube holder 32, the second shutter 36 is closed. After the shutter is completely closed, the first shutter 35 is opened to receive the weak light in the test tube at the light receiving portion 33a of the photomultiplier tube 33, and to output an electric signal (amplified current) proportional to the received light amount. Measuring unit 4
Enter 0.
【０００７】上記の２つのシャッタは電気的に制御され
るシャッタを使用して上記の操作を行っていた。特に、
第１のシャッタ３５は暗箱内に設置されているため、電
気的に制御可能なシャッタとしなければ、暗箱の外から
の開閉操作はできないため電気的制御可能なシャッタと
する必要があった。[0007] The above two shutters perform the above operations using electrically controlled shutters. In particular,
Since the first shutter 35 is installed in the dark box, unless the shutter is electrically controllable, the shutter cannot be opened and closed from the outside of the dark box.
【０００８】[0008]
【発明が解決しようとする課題】上記の従来の発光検出
部では、光電子増倍管の受光部に強い光が入射しないよ
うにする手段として、電気的に開閉されるシャッタを設
けているため、受光部と試験管の距離が長くなり、測定
感度が低下し、精度向上の阻害要因となっている。In the above-mentioned conventional light emission detecting section, a shutter which is electrically opened and closed is provided as means for preventing strong light from entering the light receiving section of the photomultiplier tube. The distance between the light receiving unit and the test tube becomes longer, the measurement sensitivity decreases, and this is a hindrance to improving the accuracy.
【０００９】また、電気的に制御されるシャッタを使用
するために電気的な制御装置が必要となり、故障原因が
増し、更には、シャッタが高価であるためコストアップ
の要因ともなっている等、解決すべき種々の課題が内在
している。In addition, the use of an electrically controlled shutter requires an electrical control device, which increases the number of causes of failures and further increases the cost because the shutter is expensive. There are various issues to be solved.
【００１０】本発明はこのような課題に鑑み、電気的に
制御されるシャッタは使用せず、しかも、測定精度が高
く信頼のある此の種の装置を提供することを目的とす
る。The present invention has been made in view of the above problems, and it is an object of the present invention to provide a reliable device of this type which does not use an electrically controlled shutter and has high measurement accuracy.
【００１１】[0011]
【課題を解決するための手段】本発明は上記の従来の技
術に有する課題に鑑み、電気的な制御によるシャッタを
使用することなく、しかも、試験管と光電子増倍管との
対向距離短縮して測定感度を向上させる手段を提供する
ものである。SUMMARY OF THE INVENTION In view of the above-mentioned problems in the prior art, the present invention reduces the opposing distance between the test tube and the photomultiplier tube without using an electrically controlled shutter. To improve the measurement sensitivity.
【００１２】しかして、本発明における課題を解決する
ための手段は、光測定対象物を試験管に入れ、該試験管
に光電子増倍管の受光部を対向させて、暗室とした測定
室内で光測定対象物の光を受光して電気信号に変換する
発光検出部と、該発光検出部の電気信号を入力して計測
する計測部とで構成した微弱発光測定装置において、前
記発光検出部は、光電子増倍管の受光部前面に光入射を
遮光する移動可能な遮光部材を設け、この遮光部材を常
時は受光部への光入射を遮光し試験管挿入時に該試験管
によって移動され受光部に光測定対象物の光が入光でき
るようにする。Means for solving the problems in the present invention are as follows. An object to be measured is placed in a test tube, and a light receiving section of a photomultiplier tube is opposed to the test tube. In a weak light emission measurement device configured by a light emission detection unit that receives light from a light measurement target and converts the light into an electric signal, and a measurement unit that inputs and measures the electric signal of the light emission detection unit, the light emission detection unit includes: A movable light-blocking member is provided in front of the light-receiving portion of the photomultiplier tube to block light from entering the light-receiving portion. This light-blocking member always blocks light from entering the light-receiving portion and is moved by the test tube when the test tube is inserted. To allow the light of the light measurement object to enter.
【００１３】このように構成することにより、電気的に
制御されるシャッタは不要となり、更にシャッタがなく
なったことにより、試験管と光電子増倍管の受光部との
距離が短縮される。With this configuration, an electrically controlled shutter becomes unnecessary, and the distance between the test tube and the light receiving portion of the photomultiplier tube is reduced by eliminating the shutter.
【００１４】また、発光検出部は、検出部基体内に形成
された測定室と、この検出部基体に設けられ、測定室と
連通して試験管を挿出入する試験管挿通孔と、前記測定
室に受光部を覗ませて検出部基体に取り付けた光電子増
倍管と、該光電子増倍管の受光部を常時遮蔽し試験管を
挿入したときに該試験管によって移動され受光部に光測
定対象物の光が入光するようにした受光手段と、検出部
基体の試験管挿通孔の入口を開閉する測定室カバーとを
備えた構成とする。[0014] The luminescence detecting section includes a measuring chamber formed in the detecting section base, a test tube insertion hole provided in the detecting section base for communicating with the measuring chamber, and inserting and removing a test tube. The photomultiplier tube attached to the detector base while looking into the light receiving unit in the room, and the light receiving unit of the photomultiplier tube is always shielded, and when the test tube is inserted, it is moved by the test tube and measured by the light receiving unit The light receiving means is configured to receive light from an object, and a measurement chamber cover for opening and closing an entrance of a test tube insertion hole of the detection unit base is provided.
【００１５】そして、前記の測定室の内壁に円筒状で光
電子増倍管対向部を切り抜いた集光用鏡を設けることに
より測定感度を上げることができる。The measurement sensitivity can be increased by providing a condensing mirror having a cylindrical shape and having a cut-out portion facing the photomultiplier tube provided on the inner wall of the measurement chamber.
【００１６】また、試験管挿通孔を断面四角状とすると
ともに、該試験管挿通孔に挿出入する試験管も断面四角
形状とし、その平面部の一面を光電子増倍管に対向させ
ることにより、試験管と光電子増倍管の受光部との距離
をより短縮できる。Further, by forming the test tube insertion hole into a rectangular cross section, the test tube to be inserted into and out of the test tube insertion hole is also formed into a rectangular cross section, and one surface of the flat portion faces the photomultiplier tube. The distance between the test tube and the light receiving section of the photomultiplier tube can be further reduced.
【００１７】更に、測定室を断面四角状となし、該四角
状の測定室の内壁側に断面コ字状の集光用鏡を、そのコ
字状の開放側を光電子増倍管に向けて配設することによ
り、より測定感度を向上することができるようになる。Further, the measuring chamber has a square cross section, and a focusing mirror having a U-shaped cross section is provided on the inner wall side of the square measuring chamber, and the open side of the U-shape is directed toward the photomultiplier tube. By arranging, the measurement sensitivity can be further improved.
【００１８】[0018]
【発明の実施の形態】以下、本発明の実施の形態を図面
によって説明する。Embodiments of the present invention will be described below with reference to the drawings.
【００１９】図１は本発明の実施の形態における全体構
成の上面図で、発光検出部１と、計測部２とにより構成
されている。FIG. 1 is a top view of the overall configuration according to an embodiment of the present invention, which comprises a light emission detection unit 1 and a measurement unit 2.
【００２０】図２は、本発明の発光検出部１の第１の実
施の形態の説明図で、その（Ａ）図および（Ｂ）図は、
図１のＡ−Ａ断面矢視図で、（Ａ）図は試験管を挿入し
て測定室カバーを閉じた状態、（Ｂ）図は測定室カバー
を開いた状態を示す。FIG. 2 is an explanatory view of a first embodiment of the light emission detecting section 1 of the present invention, wherein FIGS.
1A is a cross-sectional view taken along the line AA of FIG. 1, wherein FIG. 1A shows a state in which a test tube is inserted and a measurement chamber cover is closed, and FIG.
【００２１】また、（Ｃ）図および（Ｄ）図は、図１の
Ｂ−Ｂ断面矢視図で、（Ｃ）図は測定室カバーを閉じた
状態、（Ｄ）図は開いた状態を示している。(C) and (D) are cross-sectional views taken along the line BB in FIG. 1, (C) shows a state in which the measurement chamber cover is closed, and (D) shows an open state. Is shown.
【００２２】本発明は、従来と同様に発光検出部１と計
測部２とで構成されているが、発光検出部１に特徴を有
する。The present invention comprises a light emission detecting section 1 and a measuring section 2 as in the prior art, but is characterized by the light emission detecting section 1.
【００２３】本発明における発光検出部１は、基本的構
成として、検出部基体１２と、光電子増倍管１３と、常
時は光電子増倍管１３の受光部を遮蔽し、試験管の挿入
によって移動して遮蔽を解除して被測定物の光量を受光
させる受光手段１４と、測定室カバー１５とから構成さ
れる。The light emission detecting section 1 of the present invention basically has a structure in which the detecting section base 12, the photomultiplier tube 13, and the light receiving section of the photomultiplier tube 13 are always shielded and inserted by inserting a test tube. The light receiving means 14 is configured to release the shielding to receive the light amount of the object to be measured, and the measurement chamber cover 15.
【００２４】検出部基体１２は、垂直方向に試験管１６
を出し入れするための試験管挿通孔１７と、測定室１８
を有し、光電子増倍管１３は、この測定室１８に位置す
る検出部基体１２の外側部に取り付けられ、その受光部
１３ａは、測定室１８の外壁に設けられたガラス筒１９
に近接又は接するように配設される。The detection unit base 12 is vertically mounted on the test tube 16.
Test tube insertion hole 17 for inserting and removing
The photomultiplier tube 13 is attached to the outside of the detection unit base 12 located in the measurement room 18, and the light receiving unit 13 a is provided with a glass tube 19 provided on the outer wall of the measurement room 18.
It is arranged so as to be close to or in contact with.
【００２５】受光手段１４は、測定室１８の下部に設け
られ、ガラス筒１９内を上下動自在の遮蔽部材２０と、
該遮蔽部材２０を、常時受光部１３ａを遮蔽する位置に
押し上げるばね（スプリング）２１とからなり、試験管
１６が挿入されたとき、遮蔽部材２０は下方に押し下げ
られ試験管内の発光を受光部が受光できるようにする。The light receiving means 14 is provided at the lower part of the measuring chamber 18, and is provided with a shielding member 20 which can move up and down inside the glass tube 19.
A spring (spring) 21 that constantly pushes up the shielding member 20 to a position where the light receiving portion 13a is shielded. When the test tube 16 is inserted, the shielding member 20 is pushed down, and the light receiving portion receives light emitted from the test tube. Make it possible to receive light.
【００２６】即ち、図２（Ａ）は試験管１６を試験管挿
通孔１７に挿入して測定カバー１５を閉じた状態で、試
験管１６の先端部が測定室１８に位置し、試験管内の発
光が光電子増倍管１３の受光部１３ａが受光できる状態
となる。また、図２（Ｂ）は、試験管１６を取り出す
（又は挿入する）ために測定室カバー１５を開いた状態
で、この状態では、遮蔽部材２０はばね２１の付勢力に
よって上昇し、受光部に外部からの光が入射しないよう
に遮蔽して保護する。That is, FIG. 2A shows a state in which the test tube 16 is inserted into the test tube insertion hole 17 and the measurement cover 15 is closed, and the distal end of the test tube 16 is located in the measurement chamber 18. Light is emitted by the light receiving portion 13a of the photomultiplier tube 13. FIG. 2B shows a state in which the measurement chamber cover 15 is opened to take out (or insert) the test tube 16. In this state, the shielding member 20 rises by the urging force of the spring 21, and Shields and protects light from entering from outside.
【００２７】測定室カバー１５は、図２（Ｃ），（Ｄ）
図に示すように、一端側は軸Ｓに回動自在に軸支され、
他端側には鎖錠手段２２が設けられ、測定室カバー１５
の鎖錠，解錠をする。この鎖錠手段２２は特別な構成と
する必要が無いが、カバー開閉ボタン２３を押すことに
よって解錠するようにすると操作しやすくなる。The measuring chamber cover 15 is shown in FIGS. 2C and 2D.
As shown in the figure, one end side is rotatably supported by a shaft S,
On the other end side, a locking means 22 is provided,
Lock and unlock the lock. The locking means 22 does not need to have a special configuration, but if the cover is opened by pressing the cover open / close button 23, the operation becomes easier.
【００２８】また、測定室カバー１５の中央部の試験管
挿入孔１７を塞ぐ部分にはゴムパッキン２４が設けら
れ、図２（Ｃ）に示すように試験管１６が挿入され、測
定室カバー１５が閉められたとき測定室１８が完全に外
部の光を遮光するようにする。A rubber packing 24 is provided in the center of the measurement chamber cover 15 to close the test tube insertion hole 17, and a test tube 16 is inserted as shown in FIG. When the is closed, the measurement chamber 18 completely shields external light.
【００２９】以上のように構成されているので、試験管
１６を挿入しないときは、光電子増倍管１３の受光部１
３ａは遮蔽部材２０で完全に遮断される。次に試験管１
６が挿入され、試験管の被測定物の収容されている部分
が測定室１８内に位置したとき、遮蔽部材２０が受光部
１３ａの遮蔽を解除し、光電子増倍管は受光可能とな
る。従って、従来のようなシャッタは不要となり、シャ
ッタを電気的に制御する制御装置も不要となる。また、
従来のように受光部の前面にシャッタが無いので、受光
部を試験管に近接して設置することができるようにな
る。When the test tube 16 is not inserted, the light receiving section 1 of the photomultiplier tube 13 is constructed.
3a is completely blocked by the shielding member 20. Next, test tube 1
When the test tube 6 is inserted and the portion of the test tube in which the object to be measured is stored is located in the measurement chamber 18, the shielding member 20 releases the shielding of the light receiving portion 13a, and the photomultiplier tube can receive light. Therefore, a conventional shutter is not required, and a control device for electrically controlling the shutter is not required. Also,
Since there is no shutter on the front surface of the light receiving unit as in the related art, the light receiving unit can be installed close to the test tube.
【００３０】図３は本発明の第２の実施の形態の説明図
で、その（Ａ）図は図１のＡ−Ａ断面図、（Ｂ）図は、
そのＢ−Ｂ断面図で、いずれも試験管１６を挿入して測
定室カバーを閉じた状態を示し、（Ｃ）図は図３（Ａ）
のＣ−Ｃ断面矢視図を示す。FIG. 3 is an explanatory view of a second embodiment of the present invention. FIG. 3 (A) is a sectional view taken along line AA of FIG. 1, and FIG.
In each of the BB cross-sectional views, a state in which the test tube 16 is inserted and the measurement chamber cover is closed is shown, and FIG.
FIG.
【００３１】この第２の実施の形態は、第１の実施の形
態の測定室１８の内壁に集光用鏡２５を設けたものであ
る。この集光用鏡２５は、円筒状で受光部１３ａに対向
する部分を切り抜いて形成し、試験管内での発光を反射
し、その一部が光電子増倍管に集光させるようになし、
測定感度を上昇させる。In the second embodiment, a converging mirror 25 is provided on the inner wall of the measurement chamber 18 of the first embodiment. The converging mirror 25 is formed by cutting out a cylindrical portion facing the light receiving portion 13a, reflects light emitted in the test tube, and partially condenses the photomultiplier tube.
Increase measurement sensitivity.
【００３２】この集光用鏡２５は、通常のガラスによる
鏡で形成するのが好ましいが、表面に反射面を有するア
ルミ薄又はアルミ材質の材料、又は光沢のあるメッキ層
を施した材料でも良い。The converging mirror 25 is preferably formed of a normal glass mirror, but may be a thin aluminum or aluminum material having a reflective surface on its surface, or a material having a glossy plating layer. .
【００３３】図４は第３の実施の形態の説明図で、その
（Ａ）図は図１のＡ−Ａ断面矢視図で、試験管１６が挿
入され測定室カバーが閉じられた状態を示し、（Ｂ）は
試験管１６と光電子増倍管１３との距離関係の説明図で
ある。FIG. 4 is an explanatory view of the third embodiment, and FIG. 4A is a sectional view taken along the line AA of FIG. 1, showing a state in which the test tube 16 is inserted and the measurement chamber cover is closed. 4B is an explanatory diagram of a distance relationship between the test tube 16 and the photomultiplier tube 13.
【００３４】この第３の実施の形態においては、試験管
挿通孔１７を四角状の孔とし、試験管１６も四角状の直
方体とし、その平面部の一面が光電子増倍管１３と対向
するようにしたことに特徴を有する。このように試験管
１６を四角形状に形成すると、図４（Ｂ）に示すよう
に、試験管と光電子増倍管１３の先端（受光部）との対
向距離ｌが丸の試験管より短くでき、このことにより測
定感度を上昇することができる。In the third embodiment, the test tube insertion hole 17 is a square hole, the test tube 16 is also a rectangular parallelepiped, and one surface of the flat portion faces the photomultiplier tube 13. The feature is that When the test tube 16 is formed in a square shape in this way, as shown in FIG. 4B, the facing distance 1 between the test tube and the tip (light receiving portion) of the photomultiplier tube 13 can be made shorter than that of a round test tube. Thus, the measurement sensitivity can be increased.
【００３５】図５は本発明の第４の実施の形態の説明図
で、第３の実施の形態の測定室を断面四角状に形成し、
その内壁に断面コ字状の集光用鏡２６を設けたものであ
る。FIG. 5 is an explanatory view of a fourth embodiment of the present invention. The measuring chamber of the third embodiment is formed in a square cross section.
A converging mirror 26 having a U-shaped cross section is provided on the inner wall.
【００３６】即ち、図５に示すように、断面コ字状の開
放側を光電子増倍管１３に向けて四角形状の試験管の一
つの平面と光電子増倍管の受光部とを直接対向させる。That is, as shown in FIG. 5, one flat surface of the square test tube and the light receiving portion of the photomultiplier tube are directly opposed with the open side having a U-shaped cross section facing the photomultiplier tube 13. .
【００３７】このように形成することにより、試験管と
光電子増倍管との距離が短くなるとともに、集光用鏡２
６により試験管内の発光が反射し、その一部が光電子増
倍管１３に集光され、測定感度が更に上昇する。By forming in this manner, the distance between the test tube and the photomultiplier tube is shortened, and the focusing mirror 2 is used.
6 reflects the light emitted in the test tube, and a part of the light is collected by the photomultiplier tube 13 to further increase the measurement sensitivity.
【００３８】集光用鏡は、通常のガラス製のものが好ま
しいが、反射面を有するアルミ箔，アルミ材質の材料，
又は表面が光沢のあるメッキ層を施した材料でもよい。The light-collecting mirror is preferably made of ordinary glass, but is preferably made of an aluminum foil having a reflecting surface, an aluminum material,
Alternatively, a material having a glossy plating layer on the surface may be used.
【００３９】以上は、化学発光、生物発光のように光測
定物自体が発光する場合の微弱発光を計測する場合であ
るが、光の刺激によって起こる蛍光の場合は、検出部基
体の測定室部分に必要な光量を発光する光源を埋め込
み、測定室カバーが閉じたとき、測定カバーに押されて
閉成するスイッチを設けて、計測時に試験管内の被測定
物に光を当てて励起させて上記と同様の手段で計測す
る。The above is the case of measuring weak light emission when the light measurement object itself emits light such as chemiluminescence or bioluminescence. In the case of fluorescence generated by light stimulation, the measurement is performed in the measurement chamber portion of the detection unit base. A light source that emits the necessary amount of light is embedded, and when the measurement chamber cover is closed, a switch that is pressed and closed by the measurement cover is provided, and at the time of measurement, light is applied to the DUT in the test tube to excite it. It measures by the same means as.
【００４０】[0040]
【発明の効果】以上のように本発明は、光電子増倍管の
受光部に常時受光部への光入射を遮光する遮光部材を設
け、測定室に試験管が挿入されたとき、該試験管によっ
て遮光部材を移動して受光部を光測定対象物の光が入光
できるようにしたもので次の効果を奏する。As described above, according to the present invention, the light receiving portion of the photomultiplier tube is provided with a light shielding member for always blocking the light incident on the light receiving portion. With this configuration, the light shielding member is moved so that the light of the light measuring object can enter the light receiving portion, and the following effects are obtained.
【００４１】（１）従来のような電気的に制御するシャ
ッタを使用しないので構成が極めてシンプルとなり、ま
た電気的な制御装置が必要なくなったことと相俟って故
障が少なくなるとともにコストダウンが可能となる。(1) Since the conventional electrically controlled shutter is not used, the configuration is extremely simple. In addition to the necessity of the electric control device, the number of failures is reduced and the cost is reduced. It becomes possible.
【００４２】（２）受光部にシャッタが無いので、受光
部と試験管との距離の短縮が可能となり、測定感度を向
上することができる。(2) Since there is no shutter in the light receiving section, the distance between the light receiving section and the test tube can be shortened, and the measurement sensitivity can be improved.
【００４３】（３）第２の実施の形態のように集光用鏡
を設けて鏡の反射を利用して集光することにより、上記
の効果に加え、更に測定感度が向上する。(3) As in the second embodiment, a light collecting mirror is provided and light is collected by using the reflection of the mirror. In addition to the above-described effects, the measurement sensitivity is further improved.
【００４４】（４）第３の実施の形態のように、試験管
を断面四角形状とし、その平面部の一面を光電子増倍管
に面して配置することにより、試験管と光電子増倍管と
の対向距離をより近接することが出来、測定感度を向上
させることができ、この第３の実施の形態に、更に集光
用鏡を設けることにより測定感度をより向上させること
ができる。(4) As in the third embodiment, the test tube and the photomultiplier tube are formed by forming the test tube into a square cross section and arranging one surface of the flat portion facing the photomultiplier tube. And the measurement sensitivity can be improved, and the measurement sensitivity can be further improved by further providing a condensing mirror in the third embodiment.
【図１】本発明の実施の形態における全体構成の上面
図。FIG. 1 is a top view of an overall configuration according to an embodiment of the present invention.
【図２】本発明の発光検出部の第１の実施の形態の説明
図。 （Ａ）測定室カバー閉状態における図１のＡ−Ａ断面矢
視図。 （Ｂ）測定室カバー開状態における図１のＡ−Ａ断面矢
視図。 （Ｃ）測定室カバー閉状態における図１のＢ−Ｂ断面矢
視図。 （Ｄ）測定室カバー開状態における図１のＢ−Ｂ断面矢
視図。FIG. 2 is an explanatory diagram of a first embodiment of a light emission detection unit according to the present invention. (A) The AA sectional view of FIG. 1 in the measurement chamber cover closed state. FIG. 2B is a cross-sectional view taken along the line AA of FIG. 1 in a measurement chamber cover open state. FIG. 2C is a sectional view taken along the line BB of FIG. 1 in a measurement chamber cover closed state. (D) The BB sectional view of FIG. 1 in the measurement chamber cover open state.
【図３】本発明の発光検出部の第２の実施の形態の説明
図。 （Ａ）図１の測定室カバー閉状態におけるＡ−Ａ断面矢
視図。 （Ｂ）図１の測定室カバー閉状態におけるＢ−Ｂ断面矢
視図。 （Ｃ）図３（Ａ）のＣ−Ｃ断面矢視図。FIG. 3 is an explanatory diagram of a second embodiment of the light emission detection unit of the present invention. FIG. 2A is a cross-sectional view taken along the line AA of FIG. 1 in a measurement chamber cover closed state. FIG. 2B is a cross-sectional view taken along a line BB in a closed state of the measurement chamber cover in FIG. 1. FIG. 3C is a sectional view taken along the line CC of FIG.
【図４】本発明の発光検出部の第３の実施の形態の説明
図。 （Ａ）図１の測定室カバー閉状態におけるＡ−Ａ断面矢
視図。 （Ｂ）試験管と光電子増倍管との対向距離の説明図。FIG. 4 is an explanatory diagram of a third embodiment of the light emission detecting section of the present invention. FIG. 2A is a cross-sectional view taken along the line AA of FIG. 1 in a measurement chamber cover closed state. (B) Explanatory drawing of the opposing distance of a test tube and a photomultiplier tube.
【図５】本発明の発光検出部の第４の実施の形態の説明
図。FIG. 5 is an explanatory diagram of a fourth embodiment of the light emission detection unit of the present invention.
【図６】従来の微弱発光計測装置の説明図。 （Ａ）上面図。 （Ｂ）図６（Ａ）のＡ−Ａ断面矢視図。 （Ｃ）図６（Ａ）のＢ−Ｂ断面矢視図。FIG. 6 is an explanatory diagram of a conventional weak light emission measuring device. (A) Top view. FIG. 6B is a sectional view taken along the line AA of FIG. FIG. 7 (C) is a sectional view taken along the line BB in FIG.
１…発光検出部 ２…計測部 １２…検出部基体 １３…光電子増倍管 １４…受光手段 １５…測定室カバー １６…試験管 １７…試験管挿通孔 １８…測定室 １９…ガラス筒 ２０…遮蔽部材 ２１…ばね ２２…鎖錠手段 ２３…カバー開閉ボタン ２４…ゴムパッキン ２５，２６…集光用鏡 ３０…発光検出部 ３１…暗箱 ３２…試験管ホルダ ３３…光電子増倍管 ３４…試験管 ３５…第１のシャッタ ３６…第２のシャッタ ３７…挿入口 ４０…計測部 DESCRIPTION OF SYMBOLS 1 ... Light emission detection part 2 ... Measurement part 12 ... Detection part base 13 ... Photomultiplier tube 14 ... Light receiving means 15 ... Measurement room cover 16 ... Test tube 17 ... Test tube insertion hole 18 ... Measurement room 19 ... Glass cylinder 20 ... Shielding Member 21: Spring 22: Locking means 23: Cover opening / closing button 24: Rubber packing 25, 26: Condensing mirror 30: Light emission detecting unit 31: Dark box 32: Test tube holder 33: Photomultiplier tube 34: Test tube 35 ... first shutter 36 ... second shutter 37 ... insertion port 40 ... measuring unit
フロントページの続き Ｆターム(参考） 2G043 AA01 BA16 CA03 DA06 EA01 GA04 GB01 GB05 GB17 GB19 HA02 HA11 LA02 MA01 NA13 2G054 AA02 AB03 AB07 CA21 CA28 CE02 EA01 EA02 EA03 FA01 FA09 FA12 FA20 FA23 FA38 FB03 FB04 GA09 2G059 AA01 CC20 DD13 EE06 EE07 JJ13 KK02 LL04 Continued on the front page F-term (reference) 2G043 AA01 BA16 CA03 DA06 EA01 GA04 GB01 GB05 GB17 GB19 HA02 HA11 LA02 MA01 NA13 2G054 AA02 AB03 AB07 CA21 CA28 CE02 EA01 EA02 EA03 FA01 FA09 FA12 FA20 FA23 FA38 FB03 FB04 GA01 EE03 A01 JJ13 KK02 LL04
Claims (5)
に光電子増倍管の受光部を対向させて、暗室とした測定
室内で光測定対象物の光を受光して電気信号に変換する
発光検出部と、該発光検出部の電気信号を入力して計測
する計測部とで構成した微弱発光測定装置において、 前記発光検出部は、光電子増倍管の受光部前面に光入射
を遮光する移動可能な遮光部材を設け、この遮光部材
を、常時は受光部への光入射を遮光し試験管挿入時に該
試験管によって移動され受光部に光測定対象物の光が入
光できるようにしたことを特徴とする微弱発光計測装
置。1. A light measuring object is placed in a test tube, and a light receiving portion of a photomultiplier tube is opposed to the test tube. In a weak light emission measurement device configured by a light emission detection unit to be converted and a measurement unit that inputs and measures an electric signal of the light emission detection unit, the light emission detection unit detects light incident on a front surface of a light reception unit of a photomultiplier tube. A movable light-shielding member for shielding light is provided, and the light-shielding member is always shielded from light incident on the light receiving unit so that the light of the light measurement object can enter the light receiving unit by being moved by the test tube when the test tube is inserted. A weak light emission measuring device, characterized in that:
に光電子増倍管の受光部を対向させて、暗室とした測定
室内で光測定対象物の光を受光して電気信号に変換する
発光検出部と、該発光検出部の電気信号を入力して計測
する計測部とで構成した微弱発光測定装置において、 前記発光検出部は、検出部基体内に形成された測定室
と、この検出部基体に設けられ、測定室と連通して試験
管を挿出入する試験管挿通孔と、前記測定室に受光部を
覗ませて検出部基体に取り付けた光電子増倍管と、該光
電子増倍管の受光部を常時遮蔽し試験管を挿入したとき
に該試験管によって移動され受光部に光測定対象物の光
が入光するようにした受光手段と、検出部基体の試験管
挿通孔の入口を開閉する測定室カバーとで構成したこと
を特徴とする微弱発光測定装置。2. A light measuring object is placed in a test tube, a light receiving portion of a photomultiplier tube is opposed to the test tube, and the light of the light measuring object is received and converted into an electric signal in a dark room. In a weak light emission measuring device configured by a light emission detection unit to be converted and a measurement unit that inputs and measures an electric signal of the light emission detection unit, the light emission detection unit includes: a measurement chamber formed in a detection unit base; A test tube insertion hole provided in the detection unit base and communicating with the measurement chamber for inserting and removing a test tube; a photomultiplier tube attached to the detection unit base while allowing the measurement chamber to look into the light receiving unit; A light-receiving means for constantly blocking the light-receiving portion of the intensifier tube and moving by the test tube when the test tube is inserted so that light of an object to be measured enters the light-receiving portion; Weak emission characterized by comprising a measurement chamber cover that opens and closes the entrance of the hole Constant apparatus.
向部を切り抜いた集光用鏡を設けたことを特徴とする請
求項１又は２記載の微弱発光計測装置。3. The weak light emission measuring device according to claim 1, wherein a converging mirror having a cylindrical shape and a cut-out portion facing the photomultiplier tube is provided on an inner wall of the measuring chamber.
に該試験管挿通孔に挿出入する試験管も断面四角形状と
し、その平面部の一面を光電子増倍管に対向させたこと
を特徴とする請求項１又は２記載の微弱発光計測装置。4. A test tube insertion hole having a square cross section, a test tube inserted into and out of the test tube insertion hole also having a square cross section, and one surface of a flat portion thereof is opposed to the photomultiplier tube. The weak light emission measuring device according to claim 1 or 2, wherein
測定室の内壁側に断面コ字状の集光用鏡を、そのコ字状
の開放側を光電子増倍管に向けて配設したことを特徴と
する請求項４記載の微弱発光計測装置。5. A measuring chamber having a rectangular cross section, a converging mirror having a U-shaped cross section on the inner wall side of the rectangular measuring chamber, and a U-shaped open side of the measuring chamber facing the photomultiplier tube. The weak light emission measuring device according to claim 4, wherein the measuring device is provided.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10314225A JP2000146825A (en) | 1998-11-05 | 1998-11-05 | Feeble emission measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10314225A JP2000146825A (en) | 1998-11-05 | 1998-11-05 | Feeble emission measuring device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2000146825A true JP2000146825A (en) | 2000-05-26 |
Family
ID=18050800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10314225A Pending JP2000146825A (en) | 1998-11-05 | 1998-11-05 | Feeble emission measuring device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2000146825A (en) |
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| JP2002202254A (en) * | 2000-10-30 | 2002-07-19 | Dkk Toa Corp | Light measuring method and device therefor |
| JP2008164550A (en) * | 2006-12-29 | 2008-07-17 | Univ Of Tokushima | Fluorophotometer |
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