JP2000125779A - Feed for deodorizing animal feces and urine - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject feed capable of efficiently deodorizing a malodor caused by feces and urine of chicken, cattle, pig, pet, etc. without exerting a band influence on the health of humans and animals due to inhibition of urease derived from microorganisms in enteric canal or from plant and control of production of ammonia by making the feed include an extract of Cinnamomum zeylanicum with water much useful as cinnamon for a spice of food. SOLUTION: This feed contains an extract of Cinnamomum zeylanicum with water. The extract of Cinnamomum zeylanicum with water is obtained, for example, by sufficiently drying leaves, bark, twigs, roots, etc. of Cinnamomum zeylanicum, finely cut, grinding and extracting Cinnamomum zeylanicum with 10 times as much as Cinnamomum zeylanicum. The extract with water may be used as it is or its purified, concentrated or dried substance may be used. A deodorizing effect is obtained several days after administration by continuous administration of the feed.

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【０００１】[0001]

【発明の属する技術分野】本発明は、ケイヒの水抽出物
を有効成分として含有する動物糞尿消臭用飼料に関す
る。TECHNICAL FIELD The present invention relates to a feed for deodorizing animal manure containing an aqueous extract of cinnamon as an active ingredient.

【０００２】[0002]

【従来の技術】養鶏舎、養豚舎、養牛場等から排出され
る糞尿に基づく悪臭や、犬、猫等のペットの糞尿及び体
臭に基づく悪臭は、大きな公害問題となっており、様々
な対処策が取られているが抜本的な対策は見出されてい
ない。例えば、ある種の抗生物質を飼料と共に与え、動
物体内における病原性細菌の生育を抑えると共に、好ま
しい微生物の生育を助長して、結果として悪臭を抑える
方法、鶏糞処理場において、化学的消臭剤と消臭微生物
乃至消臭酵素液を、散布して発酵させ悪臭成分を分解す
る方法（特開平８−３１７９６６号公報）、養鶏にあた
って、消臭と病原菌対策として、ある種の枯草菌、放線
菌、酵母を含む生菌剤の使用などが知られている。2. Description of the Related Art Stench caused by manure discharged from poultry farms, pig farms, cattle farms, etc., and stench caused by manure and body odor of pets such as dogs and cats have become major pollution problems. Countermeasures have been taken, but no drastic measures have been found. For example, a method of feeding certain antibiotics together with feed to suppress the growth of pathogenic bacteria in animals and promote the growth of favorable microorganisms, thereby suppressing malodors. And a method for decomposing malodorous components by spraying and deodorizing microorganisms or a deodorizing enzyme solution (Japanese Patent Application Laid-Open No. 8-317966). Use of a probiotic agent containing yeast is known.

【０００３】[0003]

【発明が解決しようとする課題】本発明は、人畜の健康
に悪影響がなく、しかも鶏、牛、豚、ペット等の糞尿に
起因する悪臭を、効率的に消去する動物糞尿消臭用飼料
を提供する。畜舎や鶏舎における糞尿に起因する悪臭
は、アミンやアンモニア等の窒素化合物、メルカプタン
類の硫黄化合物、その他の有機酸等が主たるものである
が、特に窒素化合物は、畜糞や鶏糞の乾燥、堆肥化等の
工程の時間の経過と共にその悪臭が強まる。本発明は、
生体代謝に関与して体内で発生する悪臭、特にアンモニ
アに起因する動物糞尿の悪臭を軽減、消臭する飼料を提
供する。DISCLOSURE OF THE INVENTION The present invention provides a feed for deodorizing animal manure which has no adverse effect on human and animal health and which efficiently eliminates offensive odor caused by manure of chickens, cows, pigs, pets and the like. provide. The malodors caused by manure in livestock and poultry houses are mainly nitrogen compounds such as amines and ammonia, sulfur compounds of mercaptans, and other organic acids. The odor increases with the lapse of time. The present invention
Provided is a feed that reduces and deodorizes malodor generated in the body by participating in biological metabolism, particularly malodor of animal manure caused by ammonia.

【０００４】[0004]

【課題を解決するための手段】本発明者らは、従来より
シナモンの名前で食品の香料として多用されているケイ
ヒの水抽出物を飼料に添加して与えることにより、これ
ら動物の糞尿の悪臭が大幅に軽減されることを見出し、
この知見に基づいて本発明を完成した。即ち、本発明
は、ケイヒの水抽出物を含有する動物糞尿消臭用飼料に
関する。Means for Solving the Problems The present inventors have added an aqueous extract of cinnamon, which has been frequently used as a flavoring agent in foods under the name of cinnamon, to a feed, and given it to the malodor of manure of these animals. Is found to be significantly reduced,
The present invention has been completed based on this finding. That is, the present invention relates to a feed for deodorizing animal excrement containing an aqueous extract of cinnamon.

【０００５】悪臭の大部分を占めるアンモニアは、生体
内の尿素から嫌気性微生物の持つウレアーゼや植物由来
のウレアーゼにより生成する。そしてアンモニアは、悪
臭の原因となるばかりでなく、ヒトを含む動物に対して
毒性を示し、畜産動物が自身の***した糞尿の臭い（特
にアンモニア）によって生育環境が悪化し肥育障害を起
こす場合も多く見受けられ、出来るだけその発生を抑制
することが望まれる。本発明者らは、吸着、マスキング
といった従来からよく知られた消臭方法ではなく生体内
の尿素を二酸化炭素とアンモニアに分解する腸内微生物
の持つウレアーゼや植物由来のウレアーゼを阻害するこ
とによりアンモニアの産生を抑制し、又更には腸内環境
を整えることによって有用菌の生育を促進し糞尿中の悪
臭を取り除くものである。Ammonia, which accounts for most of the bad odor, is generated from urea in the living body by urease of anaerobic microorganisms or urease of plant origin. Ammonia not only causes odor, but also shows toxicity to animals including humans. In some cases, livestock animals deteriorate their growing environment due to the smell of their excreted manure (especially ammonia) and cause fattening disorders. Many are seen, and it is desired to suppress the occurrence as much as possible. The present inventors have proposed a method of deodorizing urea in a living body into carbon dioxide and ammonia by inhibiting intestinal microorganisms' urease and plant-derived urease, instead of a well-known deodorizing method such as adsorption and masking. It is intended to suppress the production of and to improve the intestinal environment, thereby promoting the growth of useful bacteria and removing the malodor in the manure.

【０００６】ケイヒの水抽出物の原料であるケイヒは、
クスノキ科の常緑樹であって、葉、樹皮、枝根部に芳香
を持ち、生薬として胃腸薬等に配合され、スパイスとし
て食品の賦香に広く用いられている。本発明で用いられ
る原料ケイヒは、国内産、中国産、ベトナム産ケイヒ等
特に産地を選ばない。[0006] Keihi, a raw material of the aqueous extract of Cahi, is
An evergreen tree of the camphoraceae family, which has aroma in leaves, bark and branches and roots, is used as a crude drug in gastrointestinal drugs and the like, and is widely used as a spice for flavoring foods. The raw material used in the present invention is not particularly limited in its place of origin, such as domestic, Chinese, and Vietnamese.

【０００７】又、本発明を実施するに当たって、ケイヒ
の水抽出物は、以下の如くして調製される。例えば、ケ
イヒの葉、樹皮、小枝、根などを充分に乾燥した後、細
断、細切し、これを粉砕機で粉砕、約１０倍量の水で、
必要なら摂氏約６０度乃至１００度の温水乃至熱水で水
抽出した抽出液をそのまま用いるか、使用形態によって
は更に樹脂等による精製操作を加えても良く、又濃縮、
乾燥、例えば、風乾、自然乾燥、温風乾燥、スプレード
ライ乾燥、好ましくは、凍結乾燥したものも用いられ
る。In practicing the present invention, an aqueous extract of cinnamon bark is prepared as follows. For example, after drying the leaves, bark, twigs, roots, etc. of cabbage leaves sufficiently, shred and shred them, pulverize them with a pulverizer, and with about 10 times the amount of water,
If necessary, the extract obtained by water extraction with hot water or hot water at about 60 to 100 degrees Celsius may be used as it is, or may be further purified by a resin or the like depending on the form of use.
Drying, for example, air-drying, natural drying, warm-air drying, spray-drying, preferably freeze-drying is also used.

【０００８】かくして得られたケイヒの水抽出物は、常
法により液状、固体状、ペースト状に適宜調製し、市販
飼料にそのまま添加して、或いは各種飼料原料、例え
ば、ケイ酸、各種の穀類、大豆粕、菜種粕、その他の搾
油粕等の粕類、米糠、フスマ等のヌカ類に添加混合して
飼料を調製して与える。本発明の動物糞尿消臭用飼料に
添加されるケイヒの水抽出物の使用量には特に限定はな
く、継続投与によって投与から数日後には消臭の効果が
現れる。The aqueous extract of cinnamon bark thus obtained is appropriately prepared in a liquid, solid or paste form by a conventional method and added directly to a commercial feed or as a raw material for various feeds such as silicic acid and various cereals. , Soybean meal, rapeseed meal, and other oily meals, such as lees, and rice bran, bran, and other brassica are added and mixed to prepare a feed. The amount of the aqueous extract of cabbage added to the animal manure deodorant feed of the present invention is not particularly limited, and the deodorizing effect appears several days after administration by continuous administration.

【０００９】本発明におけるケイヒの水抽出物が糞尿の
減臭、消臭効果の発生に有効であることのメカニズム
は、腸管内の微生物由来や植物由来のウレアーゼ活性の
抑制とアンモニアの産生を顕著に抑えるからであると考
えられる。The mechanism by which the aqueous extract of cabbage in the present invention is effective for deodorizing and deodorizing feces and urine is based on the suppression of microbial and plant-derived urease activity in the intestinal tract and the production of ammonia. It is considered that this is because

【００１０】[0010]

【発明実施の態様】DETAILED DESCRIPTION OF THE INVENTION

【実施例】実施例１ 細刻した広南ケイヒの乾燥物５０ｇ（日野薬品株式会社
製）を、粉砕機で粉砕、１リットルの共通すり合わせ硬
質ガラスフラスコに入れ、５００ｃｃの水を加えた後、
精油定量器を装置し、定量器の上端に還流冷却器を付け
て加熱し、沸騰させた。５時間沸騰を続けた後、加熱を
止め、常温に１時間放置した。次いで、精油部分を除去
した残りの水抽出液から固形部分を濾去し、濾液を更に
遠心分離にかけ、上澄み液をロータリーエバポレーター
で６０℃に加温し、約半量まで濃縮した。この濃縮液を
凍結乾燥して、ケイヒの水抽出乾燥物５．５６ｇを得
た。Example 1 50 g of a finely chopped dried product of Guangnan Keihi (manufactured by Hino Pharmaceutical Co., Ltd.) was pulverized with a pulverizer, placed in a 1-liter common ground hard glass flask, and after adding 500 cc of water,
An essential oil meter was installed, and a reflux condenser was attached to the upper end of the meter to heat and boil. After boiling for 5 hours, heating was stopped and the mixture was left at room temperature for 1 hour. Subsequently, the solid portion was filtered off from the remaining aqueous extract from which the essential oil portion had been removed, the filtrate was further subjected to centrifugation, and the supernatant was heated to 60 ° C. with a rotary evaporator and concentrated to about half the volume. This concentrated liquid was freeze-dried to obtain 5.56 g of a dried water extract of cinnamon bark.

【００１１】実施例2 市販配合飼料（商品名「ニュースター」日和産業株式会
社製）１０Ｋｇに、上と同様にして得られたケイヒの水
抽出物の凍結乾燥物１０ｇを添加・混合したものと、２
０ｇを添加・混合したものの２種を調製し、飼料とし
た。又、対照区には、ケイヒ水抽出物を添加しない上記
市販飼料をそのまま用いた。Example 2 10 kg of a commercially available compound feed (trade name "Newstar" manufactured by Nichiwa Sangyo Co., Ltd.) was mixed with 10 g of a freeze-dried product of the water extract of cauliflower obtained in the same manner as above. And 2
Two kinds were prepared by adding and mixing 0 g and used as feed. In the control group, the above commercial feed to which no aqueous extract of cinnamon was added was used as it was.

【００１２】鶏が***した糞便を密閉状態で集積し、一
定期間毎に北川式アンモニア検知管によりアンモニア濃
度を測定した。使用した鶏は、養鶏試験場において、１
群２０羽宛の採卵鶏を３０日間レーヤー飼育したもので
ある。測定されたアンモニア濃度を表１に示す。表１か
ら明らかな如く、本発明によって、糞尿中のアンモニア
の産生そのものを抑制することが可能となり、その消臭
効果は大きい。尚、飼育期間中、飼育鶏には各区共格別
健康状態に異常は見出されなかった。The feces excreted by the chickens were collected in a sealed state, and the ammonia concentration was measured at regular intervals by a Kitagawa ammonia detector tube. The chicken used was 1
The hens for a group of 20 birds were reared in a layer for 30 days. Table 1 shows the measured ammonia concentrations. As is clear from Table 1, the present invention makes it possible to suppress the production of ammonia itself in manure and its deodorizing effect is large. During the breeding period, no abnormalities were found in the breeding chickens in each zone.

【００１３】[0013]

【表１】 [Table 1]

【００１４】官能評価試験 実施例２における飼育場所において、消臭化度をパネラ
ー５名に判定させ、５段階臭気強度表示法により表示し
た。結果を表２に示す。Sensory Evaluation Test At the breeding place in Example 2, the degree of deodorization was judged by five panelists and indicated by a five-step odor intensity display method. Table 2 shows the results.

【００１５】[0015]

【表２】 [Table 2]

【００１６】試験例１ 上に得たケイヒ水抽出物乾燥物及び公知の各種ウレアー
ゼ活性阻害剤について、そのウレアーゼ活性阻害比較試
験を下記の如く行った。¹³Ｃ―標識尿素（９９原子％¹³
Ｃ、トレーサーテクノロジー社製）１ｍｇを、外径５ｍ
ｍのＮＭＲチューブ内に入れ、１／１５モル／リットル
濃度のリン酸ナトリウム緩衝液（ｐＨ７）６００μｌを
加えて¹³Ｃ―尿素を溶解し、¹³Ｃ―ＮＭＲ測定を行って
¹³Ｃ―尿素のシグナルを確認した後、ＮＭＲチューブを
１０分間氷冷した。¹³Ｃ―ＮＭＲのリファレンスとして
ＴＳＰ（３―トリメチルシリルプロピオン酸ナトリウム
ーd₄）を用い、ＴＳＰのシグナルを０ｐｐｍとした。Test Example 1 The dried urease extract obtained above and various known urease activity inhibitors were subjected to a urease activity inhibition comparative test as follows. ¹³ C-labeled urea (99 atom% ¹³
C, manufactured by Tracer Technology) 1 mg, outer diameter 5 m
m NMR tube, 600 μl of a sodium phosphate buffer (pH 7) at a concentration of 1/15 mol / liter was added to dissolve ¹³ C-urea, and ¹³ C-NMR measurement was performed.
After confirming the signal of ¹³ C-urea, the NMR tube was ice-cooled for 10 minutes. TSP (sodium 3-trimethylsilylpropionate-d ₄ ) was used as a reference for ¹³ C-NMR, and the signal of TSP was set to 0 ppm.

【００１７】一方、ナタ豆（ジャックビーン）由来のウ
レアーゼ（和光純薬（株）製）２００ｍｇを１／１５モ
ル／リットル濃度のリン酸ナトリウム緩衝液（ｐＨ７）
２０ｍｌ中に含有するウレアーゼ溶液を１０分間氷冷し
た後、このウレアーゼ溶液２０μｌを上記のＮＭＲチュ
ーブ内に加えて、直ちに¹³Ｃ―ＮＭＲ測定を開始し、１
分後から１３分後まで１分毎に積算回数１９回（測定時
間１分）としてＮＭＲスペクトルを測定した。On the other hand, 200 mg of urease (manufactured by Wako Pure Chemical Industries, Ltd.) derived from peanut (jack bean) was treated with a sodium phosphate buffer (pH 7) at a concentration of 1/15 mol / liter.
After the urease solution contained in 20 ml was cooled on ice for 10 minutes, 20 μl of the urease solution was added to the above-mentioned NMR tube, and ¹³ C-NMR measurement was immediately started.
From the minute to the 13th minute, the NMR spectrum was measured every minute with the number of accumulations being 19 (measurement time: 1 minute).

【００１８】反応中、尿素のシグナルは時間の経過と共
に減少し、一方、二酸化炭素のシグナルが経時的に増加
していく様子が確認できた。尚、測定終了後のｐＨは８
であった。又、測定後のＮＭＲチューブの内部から気体
を抜き取り、ＧＣ−ＭＳ測定を行ったところ、¹³Ｃ―標
識二酸化炭素（ｍ／ｚ：４５）の存在が確認できた。During the reaction, it was confirmed that the urea signal decreased with time, while the carbon dioxide signal increased with time. The pH after the measurement was 8
Met. Further, gas was extracted from the inside of the NMR tube after the measurement, and GC-MS measurement was performed. As a result, the presence of ¹³ C-labeled carbon dioxide (m / z: 45) was confirmed.

【００１９】次いで、ＮＭＲチューブにウレアーゼ溶
液、被検物質として、実施例１で得たケイヒ水抽出物乾
燥物又は公知のウレアーゼ阻害剤を表３に示す各量、及
び¹³Ｃ―標識尿素１ｍｇを加え、¹³Ｃ―ＮＭＲ測定を行
い、¹³Ｃ―標識尿素の消失時間を測定した。コントロー
ルは、被検物質を添加しない場合の測定結果である。結
果を表３に示す。Next, the urease solution was placed in an NMR tube, and the dried water extract of cinnamon water extract obtained in Example 1 or a known urease inhibitor as a test substance was added to each of the amounts shown in Table 3 and 1 mg of ¹³ C-labeled urea. In addition, ¹³ C-NMR measurement was performed to determine the disappearance time of ¹³ C-labeled urea. The control is the measurement result when no test substance is added. Table 3 shows the results.

【００２０】表３から明らかなように、ケイヒ水抽出物
乾燥物は、ビタミンＣ（アスコルビン酸）の約５倍のウ
レアーゼ阻害力を有していた。また、シャンピニョンエ
キスはビタミンＣの約１／２の阻害力を有していた。ユ
ッカエキスにはウレアーゼ阻害活性は認められなかっ
た。As is clear from Table 3, the dried water extract of cabbage had urease inhibitory activity about 5 times that of vitamin C (ascorbic acid). Moreover, the Champignon extract had about 1/2 the inhibitory power of vitamin C. No urease inhibitory activity was observed in Yucca extract.

【００２１】[0021]

【表３】 [Table 3]

【００２２】[0022]

【発明の効果】以上の如く、本発明のケイヒ水抽出物を
混和した飼料を用いて飼育することによって、動物の糞
尿の臭気が大幅に軽減、消臭が可能となり、畜舎、鶏舎
等から発生する悪臭公害を大きく軽減できる。As described above, by breeding using feed containing the aqueous extract of cabbage of the present invention, the odor of animal excreta can be significantly reduced and deodorized, and it is generated from livestock pens, poultry houses, etc. It can greatly reduce bad smell pollution.

───────────────────────────────────────────────────── フロントページの続き Ｆターム(参考） 2B005 DA01 2B150 AA01 AA02 AA03 AA05 AA06 AB12 DD31 DD32 DD42 DD57 ──────────────────────────────────────────────────続 き Continued on the front page F term (reference) 2B005 DA01 2B150 AA01 AA02 AA03 AA05 AA06 AB12 DD31 DD32 DD42 DD57

Claims (2)

【特許請求の範囲】[Claims] 【請求項１】 ケイヒの水抽出物を含有する動物糞尿消
臭用飼料。1. A feed for deodorizing animal manure comprising an aqueous extract of cinnamon. 【請求項２】 請求項１における動物糞尿消臭用飼料が
鶏糞の悪臭消臭用である動物糞尿消臭用飼料。2. The animal manure deodorant feed according to claim 1, wherein the animal manure deodorant feed is used for deodorizing chicken manure.