JP2000125776A - Liquid feed using wastewater of rice washing and its production - Google Patents
Liquid feed using wastewater of rice washing and its productionInfo
- Publication number
- JP2000125776A JP2000125776A JP10316872A JP31687298A JP2000125776A JP 2000125776 A JP2000125776 A JP 2000125776A JP 10316872 A JP10316872 A JP 10316872A JP 31687298 A JP31687298 A JP 31687298A JP 2000125776 A JP2000125776 A JP 2000125776A
- Authority
- JP
- Japan
- Prior art keywords
- lactic acid
- rice
- acid bacteria
- wastewater
- liquid feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、洗米廃水を利用す
る新しいタイプの家畜用液体飼料とその製造方法に関す
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a new type of livestock liquid feed utilizing rice washing wastewater and a method for producing the same.
【0002】[0002]
【従来の技術とその課題】精米後の洗米廃水(いわゆる
米のとぎ汁)は、水公害の一因として川や湖の汚染をも
たらす可能性があるとともに、有用成分を棄ててしまう
ことになり、経済的ロスを少なくする見地から、その対
応策が提案されている。その中でも、米をとがずに炊け
るようにした無洗米処理は、洗米に要する水の量を大幅
に減少する(100分の1)ことができるので、環境保
護の面から注目されるとともに、水の使用料を節減し、
且つ洗米による成分ロスも少なくすることができるので
コスト面からも歓迎されている。しかし、無洗米処理に
よっても廃水が完全になくなるわけではなく、無洗米処
理後の廃水は有用成分が高濃度で存在していると考えら
れるので、そのまま廃棄するのは却って有害である。ま
た、有効利用を図るために、動物の飼料に混ぜる等の用
途も考えられるが、そのままの状態では長期間の保存が
効かないので適していない。本発明の目的は、洗米廃水
の有効利用を図ることのできる新しい技術を提供するこ
とにある。2. Description of the Related Art Rice washing wastewater after polishing (so-called rice soup juice) may cause pollution of rivers and lakes as a cause of water pollution, and also discard useful components. Countermeasures have been proposed from the viewpoint of reducing economic losses. Above all, non-washing rice processing, which makes it possible to cook rice without sharpening it, can greatly reduce the amount of water required for washing rice (1/100), so it is attracting attention from the viewpoint of environmental protection, Save on water usage,
In addition, since the component loss due to rice washing can be reduced, it is welcomed in terms of cost. However, wastewater treatment does not completely eliminate wastewater, and wastewater after treatment without washing is considered to contain useful components at a high concentration. In order to use it effectively, it may be used in animal feeds, but it is not suitable because it cannot be preserved for a long period of time. An object of the present invention is to provide a new technology that can effectively utilize rice washing wastewater.
【0003】[0003]
【課題を解決するための手段】本発明は、研究を重ねた
結果、濃厚な洗米廃水を乳酸菌で発酵させることにより
家畜の飼料等として好適な液状生成物が得られることを
見出した。かくして、本発明は、精米後の米表面の有用
成分を含有する洗米廃水を用いて乳酸菌を培養してpH
が4.0以下になるまで乳酸発酵を行わせることにより、
乳酸を生産させ且つ乳酸菌を増殖させることを特徴とす
る液体飼料の製造方法を提供するものである。As a result of repeated studies of the present invention, it has been found that a liquid product suitable as feed for livestock can be obtained by fermenting concentrated rice washing wastewater with lactic acid bacteria. Thus, the present invention provides a method for culturing lactic acid bacteria using rice washing wastewater containing useful components on the surface of rice after milling to adjust pH.
Lactic acid fermentation until is less than 4.0,
An object of the present invention is to provide a method for producing a liquid feed, characterized by producing lactic acid and growing lactic acid bacteria.
【0004】本発明において特に適している洗米廃水
は、精米を無洗米処理した後の廃水である。また、本発
明において使用するのに好ましい乳酸菌は、サイレージ
用乳酸菌またはデンプン資化性乳酸菌である。[0004] The rice washing wastewater that is particularly suitable in the present invention is wastewater obtained by subjecting milled rice to a non-washing treatment. Also, preferred lactic acid bacteria for use in the present invention are lactic acid bacteria for silage or lactic acid bacteria assimilating starch.
【0005】さらに、本発明は、上記のように、洗米廃
水で乳酸菌を培養することによって得られる飼料であっ
て、pHが4.0以下であり、少なくとも約3.0g/lの
乳酸および少なくとも約1.0g/lの乳酸菌を含有する
ことを特徴とする液体飼料も提供する。Further, the present invention provides a feed obtained by culturing lactic acid bacteria in rice washing wastewater as described above, wherein the feed has a pH of 4.0 or less, at least about 3.0 g / l of lactic acid and at least about 3.0 g / l. A liquid feed comprising about 1.0 g / l of lactic acid bacteria is also provided.
【0006】[0006]
【発明の実施の形態】本発明に従い液体飼料を製造する
のに使用される洗米廃水とは、精米後の米表面の有用成
分を比較的高濃度で含有しているものであり、一般的に
は、デンプンを約0.5g/100g以上、および遊離糖
(水溶性糖)を約1.0g/l00g以上含有するととも
に、タンパク質を約1.0g/100g以上、脂質を約1.
0g/100g以上含有するものである。BEST MODE FOR CARRYING OUT THE INVENTION The rice washing wastewater used for producing a liquid feed according to the present invention contains a relatively high concentration of useful components on the surface of rice after rice polishing, and is generally Contains not less than about 0.5 g / 100 g of starch, not less than about 1.0 g / 100 g of free sugar (water-soluble sugar), not less than about 1.0 g / 100 g of protein, and not more than about 1.0 g of lipid.
The content is 0 g / 100 g or more.
【0007】このような条件を満たす洗米廃水として特
に好ましいのは、精米を無洗米処理する際に得られる廃
水である。最近、精米を無洗米処理するための幾つかの
方法が提案されているが、本発明に好適な無洗米処理の
例は、精米と連動させた製造装置内で、精米したての白
米にその重量に対して15%の霧状の水を噴き付け、白
米表面に霧状の水と糠が付いた状態で搗精、遠心脱水機
で糠含みの水を取り除いたあと、乾燥させて仕上げたも
のである。この時に副生する水を用いれば、そのまま乳
酸菌を培養して液体飼料を製造することができる。[0007] Particularly preferred as rice washing wastewater satisfying such conditions is wastewater obtained when non-washed rice is treated. Recently, several methods for treating rice without washing have been proposed. An example of the treatment that is suitable for the present invention is to produce freshly-polished white rice in a production apparatus linked to rice polishing. Sprayed with 15% mist water based on the weight, polished with the mist water and bran on the surface of white rice, removed the water containing bran with a centrifugal dehydrator, and dried and finished It is. At this time, if water produced as a by-product is used, lactic acid bacteria can be cultured as it is to produce a liquid feed.
【0008】例示したような無洗米処理法以外の方法に
よって得られる洗米廃水を用いて本発明の方法を実施す
ることもできる。但し、洗米廃水中に有用成分が上述し
た程度に含有されていない場合は、そのまま乳酸菌の培
養に使用することはできず、該洗米廃水を濃縮してから
乳酸菌を培養することが必要である。[0008] The method of the present invention can also be carried out using rice washing wastewater obtained by a method other than the non-washing rice treatment method as exemplified. However, when the useful component is not contained in the rice washing wastewater to the above-mentioned extent, it cannot be used for culturing lactic acid bacteria as it is, and it is necessary to culture the lactic acid bacteria after concentrating the rice washing wastewater.
【0009】本発明に従えば、上記のような無洗米処理
廃水に代表される洗米廃水を用いて乳酸菌を培養し乳酸
発酵を行わせることにより家畜の飼料として好適な液体
飼料が得られる。培養は、前培養の後、30〜35℃の
温度下に系のpHが4.0またはそれ以下になるまで(通
常、10時間以上)実施することが必要であり、これに
よって、充分量の乳酸および菌体(乳酸菌)を含有し、
しかも長期間保存することのできる液体飼料が得られ
る。According to the present invention, a liquid feed suitable as a feed for livestock can be obtained by culturing lactic acid bacteria using fermented rice wastewater typified by the above-mentioned non-washed rice treated wastewater and performing lactic acid fermentation. After the pre-culture, it is necessary to carry out the culture at a temperature of 30 to 35 ° C. until the pH of the system becomes 4.0 or lower (generally, 10 hours or more). Contains lactic acid and bacteria (lactic acid bacteria)
In addition, a liquid feed that can be stored for a long time is obtained.
【0010】本発明の方法は、原理的には各種の乳酸菌
を用いて実施することができるが、特に好ましい乳酸菌
の例は、サイレージ用乳酸菌またはデンプン資化性乳酸
菌である。前者の例は、Lactobacillus plantarum で
あり、香りや味など特に家畜の嗜好性に適した飼料を製
造することが所望される場合に使用される。デンプン資
化性の乳酸菌として好ましい例は、Lactobacillus am
ylovorusであり、これは乳酸と菌体量が多く且つpHが
充分に低くて、特に有効性や保存生に優れた液体飼料を
得る場合に適している。Although the method of the present invention can be carried out using various lactic acid bacteria in principle, examples of particularly preferred lactic acid bacteria are lactic acid bacteria for silage or starch-utilizing lactic acid bacteria. An example of the former is Lactobacillus plantarum, which is used when it is desired to produce a feed that is particularly suitable for livestock taste, such as aroma and taste. Preferred examples of starch-utilizing lactic acid bacteria include Lactobacillus am
ylovorus , which is high in lactic acid and bacterial mass and has a sufficiently low pH, and is particularly suitable for obtaining a liquid feed excellent in effectiveness and storage life.
【0011】本発明に従い洗米廃水を乳酸発酵して得ら
れる液状生成物は、pHが4.0以下であり、殆どの雑菌
を阻害することが確認されている。したがって、この液
状生成物は、そのまま室温に放置しても長期間にわたっ
て(少なくとも半年間)腐敗や変質することもないの
で、液体飼料として保存生に優れている。The liquid product obtained by lactic acid fermentation of rice washing wastewater according to the present invention has a pH of 4.0 or less, and it has been confirmed that most of the various bacteria are inhibited. Therefore, this liquid product does not spoil or deteriorate over a long period of time (at least half a year) even when left at room temperature, so that it is excellent in storage life as a liquid feed.
【0012】さらに、本発明によって得られるこのよう
な液体飼料は、充分な量の乳酸(少なくとも3.0g/
l)と乳酸菌(少なくとも1.0g/l)を含有するの
で、そのまま、または他の飼料と混合して、ウシなどの
家畜に与えると、その消化を助け整腸作用を促進して家
畜の健全な飼育に資することも見出されている。In addition, such a liquid feed obtained according to the present invention has a sufficient amount of lactic acid (at least 3.0 g / l).
l) and lactic acid bacteria (at least 1.0 g / l). When given to livestock such as cows as they are or mixed with other feeds, they help digestion and promote intestinal regulation to promote livestock health. Has been found to contribute to safe breeding.
【0013】[0013]
【実施例】以下、本発明の特徴をさらに明らかにするた
め実施例を示すが、本発明はこれらの実施例によって制
限されるものではない。実施例1:サイレージ用乳酸菌による乳酸発酵 サイレージ用乳酸菌Lactobacillus plantarum の保存
菌〔Institute for Fermentation, Osaka (IFO:(財)
発酵研究所)よりIFO3070として入手〕を前培養
培地(酵母エキス:0.25%、ポリペプトン:0.5%、
ブドウ糖:0.1%)で培養した後、前培養液1リットル
を20リットルの洗米廃水に接種し、30〜35℃で静
置培養した。用いた洗米廃水は、島根産コシヒカリを前
述のごとき無洗米処理することによって得られた水であ
り、その組成は次のとおりであった:遊離糖(1.4g/
l00g)、デンプン(0.91g/100g)、タンパ
ク質(1.36g/100g)、脂質(1.34g/100
g)、灰分(0.94g/100g)、固形分(65g/
リットル)、BOD(68g/リットル)、COD(5
4g/リットル)。EXAMPLES The following examples are provided to further clarify the features of the present invention, but the present invention is not limited by these examples. Example 1: Lactic acid fermentation by lactic acid bacteria for silage Preservative bacteria of lactic acid bacteria Lactobacillus plantarum for silage [Institute for Fermentation, Osaka (IFO:
Fermentation laboratory) as a preculture medium (yeast extract: 0.25%, polypeptone: 0.5%,
(Glucose: 0.1%), 1 liter of the pre-cultured liquid was inoculated into 20 liters of rice washing wastewater, and cultured at 30 to 35 ° C. The used rice washing wastewater was water obtained by subjecting Shimane-produced Koshihikari to the above-described non-washing rice treatment, and its composition was as follows: free sugar (1.4 g /
100 g), starch (0.91 g / 100 g), protein (1.36 g / 100 g), lipid (1.34 g / 100 g)
g), ash (0.94 g / 100 g), solid content (65 g /
Liter), BOD (68 g / liter), COD (5
4 g / liter).
【0014】なお、遊離糖は、「Somogyi-Nelson法」に
よって分析した。すなわち、適宜稀釈した試料に銅試薬
を加え、加熱後、急冷し、Nelson試薬を加えた後、その
際発する青緑色を、光電比色計で波長660nmの吸光
度を測定し、標準曲線から遊離糖量を求めた。また、そ
の他の成分の分析法または分析機器は次のとおりであ
る:デンプン(用手法)、タンパク質(たんぱく自動分
析装置、TECATOR 社製1035 ANALYZER)、脂質( 電子天
秤、メトラー社製AE-240) 、固形分( 電子天秤、ザルト
リウス社製1702MP8)、BOD(BOD 自動測定装置、ラボ
テック社製KI-100-B3AおよびDO-300-40A) 、COD(用
手法)。The free sugar was analyzed by the "Somogyi-Nelson method". That is, a copper reagent was added to a sample diluted appropriately, heated, quenched, and Nelson's reagent was added. The blue-green color generated at that time was measured for the absorbance at a wavelength of 660 nm with a photoelectric colorimeter, and the free sugar was determined from the standard curve. The amount was determined. In addition, the analytical methods or analytical instruments for other components are as follows: starch (method for use), protein (automated protein analyzer, 1035 ANALYZER manufactured by TECATOR), lipid (electronic balance, METTLER AE-240) , Solid content (electronic balance, 1702MP8 manufactured by Sartorius), BOD (BOD automatic measuring device, KI-100-B3A and DO-300-40A manufactured by Labotech), COD (method for use).
【0015】培養結果を表1に示す。この表から理解さ
れるように、洗米廃水がそのまま乳酸菌の培地として利
用されて乳酸発酵が進行しており、培養12時間でpH
が4.0に低下し殆どの雑菌の増殖を阻害することができ
た。Table 1 shows the results of the culture. As can be understood from this table, rice washing wastewater is used as it is as a medium for lactic acid bacteria, and lactic acid fermentation is progressing.
Was reduced to 4.0, and the growth of most bacteria could be inhibited.
【0016】[0016]
【表1】 [Table 1]
【0017】実施例2:デンプン資化性乳酸菌による乳
酸発酵 デンプン資化性乳酸菌Lactobacillus amylovorusの保
存菌〔Japan Collection of Microorganism, Institute
of Physical and Chemical Research (Hirosawa, Wak
o) からJCM1126として入手〕を前培養培地(酵
母エキス:0.25%、ポリペプトン:0.5%、ブドウ
糖:0.1%)で培養した後、前培養液1リットルを実施
例1で用いたのと同じ洗米廃水20リットルに接種し、
30〜35℃で静置培養した。 Example 2 Milk with starch-utilizing lactic acid bacteria
Preservative bacteria of Lactobacillus amylovorus, a lactic acid bacterium that can utilize acid-fermented starch (Japan Collection of Microorganism, Institute
of Physical and Chemical Research (Hirosawa, Wak
o) as JCM1126] in a pre-culture medium (yeast extract: 0.25%, polypeptone: 0.5%, glucose: 0.1%), and 1 liter of the pre-cultured solution was used in Example 1. Inoculate the same 20 liters of rice washing wastewater
Stationary culture was performed at 30 to 35 ° C.
【0018】その培養結果を表2に示す。デンプン資化
性乳酸菌を用いると、乳酸生成や菌体増殖の速度は少し
遅くなるが、実施例1のようなサイレージ用乳酸菌を用
いる場合に比べてpHを低くすることができ且つ乳酸生
成量も増大し、保存性や活性の優れた液状生成物を得る
ことができることが理解される。Table 2 shows the results of the culture. The use of starch-utilizing lactic acid bacteria slightly lowers the rate of lactic acid generation and cell growth, but can lower the pH and the amount of lactic acid production as compared with the case of using lactic acid bacteria for silage as in Example 1. It is understood that a liquid product with increased preservability and activity can be obtained.
【0019】[0019]
【表2】 [Table 2]
【0020】なお、実施例1および実施例2における乳
酸生成量および菌体量の分析は、以下のように行った。 乳酸生成量:「Berker & Summerson法」によって分析し
た。すなわち、濃硫酸で試料中の乳酸をアセトアルデヒ
ドに酸化し、第一銅イオンの存在下で、ρ−ヒドロキシ
ジフェニルを加えて、その際に発する青紫色を光電比色
計で波長570nmの吸光度を測定し、標準曲線から乳
酸量を求めた。 菌体量:培養液を10倍に稀釈し、その濁度を光電比色
計で波長660nmの吸光度を測定し、予め作成した濁
度と菌体の乾燥重量との相関関係を示す標準曲線から菌
体量を算出した。The analysis of the amount of lactic acid produced and the amount of cells in Examples 1 and 2 were performed as follows. Lactic acid production: analyzed by "Berker & Summerson method". That is, lactic acid in the sample is oxidized to acetaldehyde with concentrated sulfuric acid, ρ-hydroxydiphenyl is added in the presence of cuprous ion, and the blue-violet color emitted at that time is measured for absorbance at a wavelength of 570 nm with a photoelectric colorimeter. Then, the amount of lactic acid was determined from the standard curve. Cell quantity: The culture solution was diluted 10-fold, and the turbidity was measured for absorbance at a wavelength of 660 nm using a photoelectric colorimeter. Based on a standard curve showing the correlation between the previously prepared turbidity and the dry weight of the cells. The cell mass was calculated.
【0021】実施例3:家畜への投与試験 実施例2の乳酸発酵によって得られた液状生成物を飼料
として乳牛(3才)に毎日、朝夕に1.5リットルずつを
与え、半年間観察した。対照として、該液体飼料を与え
ない同じ年齢の乳牛についても観察を行った。 Example 3: Dosage test on livestock The dairy cow (3 years old) was fed with the liquid product obtained by the lactic acid fermentation of Example 2 as a feed daily, 1.5 liters in the morning and evening, and observed for half a year. . As a control, observations were also made on dairy cows of the same age who did not receive the liquid feed.
【0022】その結果、洗米廃水を乳酸発酵させた液体
飼料を与えられた乳牛の方が明らかに、下痢が少なく、
夏場における乳量の低下がなく、さらに、フンの匂いも
少ないことが認められた。このように本発明の液体飼料
は、乳酸および乳酸菌によりウシの消化を助け腸の働き
を活性化しているものと推測される。As a result, the dairy cows fed the liquid feed obtained by lactic acid fermentation of the rice washing wastewater clearly had less diarrhea,
It was confirmed that there was no decrease in milk yield in summer and that the smell of feces was small. Thus, it is presumed that the liquid feed of the present invention assists digestion of cattle by lactic acid and lactic acid bacteria and activates the function of the intestine.
Claims (5)
米廃水を用いて乳酸菌を培養してpHが4.0以下になる
まで乳酸発酵を行わせることにより、乳酸を生産させ且
つ乳酸菌を増殖させることを特徴とする液体飼料の製造
方法。1. Lactic acid bacteria are cultivated using rice washing wastewater containing useful components on the surface of polished rice, and lactic acid fermentation is carried out until the pH becomes 4.0 or less, whereby lactic acid is produced and lactic acid bacteria are produced. A method for producing a liquid feed, wherein the method comprises growing.
廃水であることを特徴とする請求項1の液体飼料の製造
方法。2. The method for producing a liquid feed according to claim 1, wherein the rice washing wastewater is wastewater obtained by subjecting milled rice to non-washing treatment.
を特徴とする請求項1または請求項2の液体飼料の製造
方法。3. The method for producing a liquid feed according to claim 1, wherein the lactic acid bacteria are lactic acid bacteria for silage.
とを特徴とする請求項1または請求項2の液体飼料の製
造方法。4. The method for producing a liquid feed according to claim 1, wherein the lactic acid bacterium is a starch-assimilating lactic acid bacterium.
て得られ、pHが4.0以下であり、少なくとも約3.0g
/lの乳酸および少なくとも約1.0g/lの乳酸菌を含
有することを特徴とする液体飼料。5. A lactic acid bacterium obtained by culturing lactic acid bacteria in rice washing wastewater, wherein the pH is 4.0 or less and at least about 3.0 g.
/ L lactic acid and at least about 1.0 g / l lactic acid bacteria.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10316872A JP2000125776A (en) | 1998-10-20 | 1998-10-20 | Liquid feed using wastewater of rice washing and its production |
Applications Claiming Priority (1)
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JP10316872A JP2000125776A (en) | 1998-10-20 | 1998-10-20 | Liquid feed using wastewater of rice washing and its production |
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Cited By (6)
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JP2005074423A (en) * | 2003-09-03 | 2005-03-24 | Hide Kume | Method for biologically treating high concentration organic waste liquid |
JP2008005833A (en) * | 2006-05-29 | 2008-01-17 | Masaaki Tsugeta | Silage and method for preparing the same |
JP2009543545A (en) * | 2006-07-14 | 2009-12-10 | ローン レガース | Homo fermentation product |
CN103109973A (en) * | 2013-02-28 | 2013-05-22 | 广西出入境检验检疫局检验检疫技术中心 | Method for preparing lactic acid bacteria feed additive by using eleocharis tuberosa starch wastewater |
JP5314421B2 (en) * | 2006-05-31 | 2013-10-16 | 株式会社明治 | Culture method of lactic acid bacteria with high immunomodulating activity |
CN113215051A (en) * | 2021-05-26 | 2021-08-06 | 南宁市拜欧生物工程有限责任公司 | Method for preparing feed probiotics by using lactobacillus through rice flour wastewater and passion fruit peel |
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1998
- 1998-10-20 JP JP10316872A patent/JP2000125776A/en active Pending
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2005074423A (en) * | 2003-09-03 | 2005-03-24 | Hide Kume | Method for biologically treating high concentration organic waste liquid |
JP2008005833A (en) * | 2006-05-29 | 2008-01-17 | Masaaki Tsugeta | Silage and method for preparing the same |
JP5314421B2 (en) * | 2006-05-31 | 2013-10-16 | 株式会社明治 | Culture method of lactic acid bacteria with high immunomodulating activity |
JP2009543545A (en) * | 2006-07-14 | 2009-12-10 | ローン レガース | Homo fermentation product |
US10098369B2 (en) | 2006-07-14 | 2018-10-16 | Lone Legarth | Homofermented products |
CN103109973A (en) * | 2013-02-28 | 2013-05-22 | 广西出入境检验检疫局检验检疫技术中心 | Method for preparing lactic acid bacteria feed additive by using eleocharis tuberosa starch wastewater |
CN113215051A (en) * | 2021-05-26 | 2021-08-06 | 南宁市拜欧生物工程有限责任公司 | Method for preparing feed probiotics by using lactobacillus through rice flour wastewater and passion fruit peel |
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