IT201700094210A1 - Metodo per produrre lembi cellulari - Google Patents

Metodo per produrre lembi cellulari

Info

Publication number
IT201700094210A1
IT201700094210A1 IT102017000094210A IT201700094210A IT201700094210A1 IT 201700094210 A1 IT201700094210 A1 IT 201700094210A1 IT 102017000094210 A IT102017000094210 A IT 102017000094210A IT 201700094210 A IT201700094210 A IT 201700094210A IT 201700094210 A1 IT201700094210 A1 IT 201700094210A1
Authority
IT
Italy
Prior art keywords
lembi
cellulars
produce
produce lembi
lembi cellulars
Prior art date
Application number
IT102017000094210A
Other languages
English (en)
Inventor
Graziella Pellegrini
Andrea Alessandrini
Luca Michele De
Original Assignee
Holostem Terapie Avanzate S R L
Univ Degli Studi Di Modena E Reggio Emilia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Holostem Terapie Avanzate S R L, Univ Degli Studi Di Modena E Reggio Emilia filed Critical Holostem Terapie Avanzate S R L
Priority to IT102017000094210A priority Critical patent/IT201700094210A1/it
Priority to PCT/EP2018/071736 priority patent/WO2019034550A1/en
Priority to EP18750219.0A priority patent/EP3668967A1/en
Priority to US16/639,955 priority patent/US20200291353A1/en
Publication of IT201700094210A1 publication Critical patent/IT201700094210A1/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/56—Fibrin; Thrombin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IT102017000094210A 2017-08-17 2017-08-17 Metodo per produrre lembi cellulari IT201700094210A1 (it)

Priority Applications (4)

Application Number Priority Date Filing Date Title
IT102017000094210A IT201700094210A1 (it) 2017-08-17 2017-08-17 Metodo per produrre lembi cellulari
PCT/EP2018/071736 WO2019034550A1 (en) 2017-08-17 2018-08-10 PROCESS FOR PRODUCING CELL FLAPS
EP18750219.0A EP3668967A1 (en) 2017-08-17 2018-08-10 Method for producing cell flaps
US16/639,955 US20200291353A1 (en) 2017-08-17 2018-08-10 Method for producing cell flaps

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT102017000094210A IT201700094210A1 (it) 2017-08-17 2017-08-17 Metodo per produrre lembi cellulari

Publications (1)

Publication Number Publication Date
IT201700094210A1 true IT201700094210A1 (it) 2019-02-17

Family

ID=60991186

Family Applications (1)

Application Number Title Priority Date Filing Date
IT102017000094210A IT201700094210A1 (it) 2017-08-17 2017-08-17 Metodo per produrre lembi cellulari

Country Status (4)

Country Link
US (1) US20200291353A1 (it)
EP (1) EP3668967A1 (it)
IT (1) IT201700094210A1 (it)
WO (1) WO2019034550A1 (it)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134093A1 (en) * 2002-09-06 2006-06-22 Dfb Pharmaceuticals, Inc. Fibrin cell supports and methods of use thereof
WO2017120147A1 (en) * 2016-01-04 2017-07-13 The Board Of Trustees Of The Leland Stanford Junior University Gene therapy for recessive dystrophic epidermolysis bullosa using genetically corrected autologous keratinocytes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2539613A1 (en) 2003-09-19 2005-03-31 Keio University Compositions for use in coating of substrate for cell sheet preparation, substrates for cell sheet preparation, and method for manufacturing cell sheets
WO2008082821A1 (en) * 2006-11-28 2008-07-10 University Of Southern California Method for promoting wound healing
EP2151248A1 (en) * 2008-07-30 2010-02-10 Johann Bauer Improved pre-mRNA trans-splicing molecule (RTM) molecules and their uses

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134093A1 (en) * 2002-09-06 2006-06-22 Dfb Pharmaceuticals, Inc. Fibrin cell supports and methods of use thereof
WO2017120147A1 (en) * 2016-01-04 2017-07-13 The Board Of Trustees Of The Leland Stanford Junior University Gene therapy for recessive dystrophic epidermolysis bullosa using genetically corrected autologous keratinocytes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
FULVIO MAVILIO ET AL: "Correction of junctional epidermolysis bullosa by transplantation of genetically modified epidermal stem cells", NATURE MEDICINE, vol. 12, no. 12, 1 January 2007 (2007-01-01), pages 1397 - 1402, XP055355723, ISSN: 1078-8956, DOI: 10.1038/nm1504 *
JOHANN W. BAUER ET AL: "Closure of a Large Chronic Wound through Transplantation of Gene-Corrected Epidermal Stem Cells", THE JOURNAL OF INVESTIGATIVE DERMATOLOGY : OFFICIAL JOURNAL OF THE SOCIETY FOR INVESTIGATIVE DERMATOLOGY AND THE EUROPEAN SOCIETY FOR DERMATOLOGICAL RESEARCH, vol. 137, no. 3, 1 March 2017 (2017-03-01), US, pages 778 - 781, XP055472361, ISSN: 0022-202X, DOI: 10.1016/j.jid.2016.10.038 *
PELLEGRINI G ET AL: "THE CONTROL OF EPIDERMAL STEM CELLS (HOLOCLONES) IN THE TREATMENT OF MASSIVE FULL-THICKNESS BURNS WITH AUTOLOGOUS KERATINOCYTES CULTURED ON FIBRIN", TRANSPLANTA, WILLIAMS AND WILKINS, GB, vol. 68, no. 6, 27 September 1999 (1999-09-27), pages 868 - 879, XP008062490, ISSN: 0041-1337, DOI: 10.1097/00007890-199909270-00021 *
STEFANO FERRARI ET AL: "Gene therapy in combination with tissue engineering to treat epidermolysis bullosa", EXPERT OPINION ON BIOLOGICAL THERAPY, vol. 6, no. 4, 21 March 2006 (2006-03-21), ASHLEY, LONDON; GB, pages 367 - 378, XP055472684, ISSN: 1471-2598, DOI: 10.1517/14712598.6.4.367 *
TOBIAS HIRSCH ET AL: "Regeneration of the entire human epidermis using transgenic stem cells", NATURE, vol. 551, no. 7680, 8 November 2017 (2017-11-08), London, pages 327 - 332, XP055472440, ISSN: 0028-0836, DOI: 10.1038/nature24487 *

Also Published As

Publication number Publication date
WO2019034550A1 (en) 2019-02-21
US20200291353A1 (en) 2020-09-17
EP3668967A1 (en) 2020-06-24

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