IT201700035315A1 - Dispositivo e metodo di produzione e purificazione di esosomi - Google Patents

Dispositivo e metodo di produzione e purificazione di esosomi

Info

Publication number
IT201700035315A1
IT201700035315A1 IT102017000035315A IT201700035315A IT201700035315A1 IT 201700035315 A1 IT201700035315 A1 IT 201700035315A1 IT 102017000035315 A IT102017000035315 A IT 102017000035315A IT 201700035315 A IT201700035315 A IT 201700035315A IT 201700035315 A1 IT201700035315 A1 IT 201700035315A1
Authority
IT
Italy
Prior art keywords
exosomes
purification
production
Prior art date
Application number
IT102017000035315A
Other languages
English (en)
Inventor
Gabriella Andriolo
Elena Provasi
Andrea Brambilla
Cicero Viviana Giovanna Lo
Sabrina Soncin
Lucio Barile
Giuseppe Vassalli
Lucia Turchetto
Marina Radrizzani
Original Assignee
Foundation For Cardiological Res And Education Fcre
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Foundation For Cardiological Res And Education Fcre filed Critical Foundation For Cardiological Res And Education Fcre
Priority to IT102017000035315A priority Critical patent/IT201700035315A1/it
Priority to US16/499,532 priority patent/US11639490B2/en
Priority to PCT/EP2017/084747 priority patent/WO2018177583A1/en
Publication of IT201700035315A1 publication Critical patent/IT201700035315A1/it

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1329—Cardiomyocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1315—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from cardiomyocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cardiology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Rheumatology (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Vascular Medicine (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
IT102017000035315A 2017-03-30 2017-03-30 Dispositivo e metodo di produzione e purificazione di esosomi IT201700035315A1 (it)

Priority Applications (3)

Application Number Priority Date Filing Date Title
IT102017000035315A IT201700035315A1 (it) 2017-03-30 2017-03-30 Dispositivo e metodo di produzione e purificazione di esosomi
US16/499,532 US11639490B2 (en) 2017-03-30 2017-12-28 Device and method for producing and purifying exosomes
PCT/EP2017/084747 WO2018177583A1 (en) 2017-03-30 2017-12-28 Device and method for producing and purifying exosomes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT102017000035315A IT201700035315A1 (it) 2017-03-30 2017-03-30 Dispositivo e metodo di produzione e purificazione di esosomi

Publications (1)

Publication Number Publication Date
IT201700035315A1 true IT201700035315A1 (it) 2018-09-30

Family

ID=59700048

Family Applications (1)

Application Number Title Priority Date Filing Date
IT102017000035315A IT201700035315A1 (it) 2017-03-30 2017-03-30 Dispositivo e metodo di produzione e purificazione di esosomi

Country Status (1)

Country Link
IT (1) IT201700035315A1 (it)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6812023B1 (en) * 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
WO2014028493A2 (en) * 2012-08-13 2014-02-20 Cedars-Sinai Medical Center Exosomes and micro-ribonucleic acids for tissue regeneration
WO2016090183A1 (en) * 2014-12-03 2016-06-09 Capricor Therapeutics, Inc. Processes for producing stable exosome formulations

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6812023B1 (en) * 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
WO2014028493A2 (en) * 2012-08-13 2014-02-20 Cedars-Sinai Medical Center Exosomes and micro-ribonucleic acids for tissue regeneration
WO2016090183A1 (en) * 2014-12-03 2016-06-09 Capricor Therapeutics, Inc. Processes for producing stable exosome formulations

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
AHMED GAMAL-ELDIN IBRAHIM ET AL: "Exosomes as Critical Agents of Cardiac Regeneration Triggered by Cell Therapy", STEM CELL REPORTS, vol. 2, no. 5, 1 May 2014 (2014-05-01), United States, pages 606 - 619, XP055335389, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2014.04.006 *
CHEN LIJUAN ET AL: "Cardiac progenitor-derived exosomes protect ischemic myocardium from acute ischemia/reperfusion injury", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 431, no. 3, 11 January 2013 (2013-01-11), pages 566 - 571, XP028980130, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2013.01.015 *
EMILY ZERINGER ET AL: "Strategies for Isolation of Exosomes", COLD SPRING HARBOR PROTOCOL, vol. 2015, no. 4, 1 April 2015 (2015-04-01), United States, XP055417515, ISSN: 1940-3402, DOI: 10.1101/pdb.top074476 *
FRANCESCO ANGELINI ET AL: "Exosomes isolation protocols facts and artifacts for cardiac regeneration", FRONTIERS IN BIOSCIENCE (SCHOLAR EDITION), vol. 8, no. 2, 1 June 2016 (2016-06-01), Searington, US, pages 303 - 311, XP055417663, ISSN: 1945-0516, DOI: 10.2741/s464 *
L. BARILE ET AL: "Extracellular vesicles from human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction", CARDIOVASCULAR RESEARCH, vol. 103, no. 4, 11 July 2014 (2014-07-11), GB, pages 530 - 541, XP055417520, ISSN: 0008-6363, DOI: 10.1093/cvr/cvu167 *
LUCIO BARILE ET AL: "Roles of exosomes in cardioprotection", EUROPEAN HEART JOURNAL, 21 July 2016 (2016-07-21), GB, pages ehw304, XP055417503, ISSN: 0195-668X, DOI: 10.1093/eurheartj/ehw304 *
MITJA L. HEINEMANN ET AL: "Benchtop isolation and characterization of functional exosomes by sequential filtration", JOURNAL OF CHROMATOGRAPHY A, vol. 1371, 1 December 2014 (2014-12-01), AMSTERDAM, NL, pages 125 - 135, XP055372708, ISSN: 0021-9673, DOI: 10.1016/j.chroma.2014.10.026 *
PIN LI ET AL: "Progress in Exosome Isolation Techniques", THERANOSTICS, vol. 7, no. 3, 1 January 2017 (2017-01-01), AU, pages 789 - 804, XP055417509, ISSN: 1838-7640, DOI: 10.7150/thno.18133 *
RAFAL SZATANEK ET AL: "Isolation of extracellular vesicles: Determining the correct approach (Review)", INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, vol. 36, no. 1, 1 July 2015 (2015-07-01), GR, pages 11 - 17, XP055409099, ISSN: 1107-3756, DOI: 10.3892/ijmm.2015.2194 *

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