IT1262937B - Selezione genica in vitro mediante vettori ad rna - Google Patents

Selezione genica in vitro mediante vettori ad rna

Info

Publication number
IT1262937B
IT1262937B ITMI920892A ITMI920892A IT1262937B IT 1262937 B IT1262937 B IT 1262937B IT MI920892 A ITMI920892 A IT MI920892A IT MI920892 A ITMI920892 A IT MI920892A IT 1262937 B IT1262937 B IT 1262937B
Authority
IT
Italy
Prior art keywords
gene selection
vitro gene
rna carriers
rna
carriers
Prior art date
Application number
ITMI920892A
Other languages
English (en)
Original Assignee
Enichem Spa
Donegani Guido Ist
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enichem Spa, Donegani Guido Ist filed Critical Enichem Spa
Priority to ITMI920892A priority Critical patent/IT1262937B/it
Publication of ITMI920892A0 publication Critical patent/ITMI920892A0/it
Priority to EP93201054A priority patent/EP0566198A2/en
Priority to JP5085871A priority patent/JPH06233681A/ja
Publication of ITMI920892A1 publication Critical patent/ITMI920892A1/it
Application granted granted Critical
Publication of IT1262937B publication Critical patent/IT1262937B/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Abstract

Viene, descritto un metodo per l'identificazione e/o isolamento di trascritti genici rari basato sull'utilizzazione di un particolare vettore, per l'inserimento di sequenze geniche, costituito dal prodotto della trascrizione del gene (formula) di lievito posto sotto il controllo del promotore della RNA polimerasi del fago T7.
ITMI920892A 1992-04-13 1992-04-13 Selezione genica in vitro mediante vettori ad rna IT1262937B (it)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ITMI920892A IT1262937B (it) 1992-04-13 1992-04-13 Selezione genica in vitro mediante vettori ad rna
EP93201054A EP0566198A2 (en) 1992-04-13 1993-04-13 In vitro gene selection by means of RNA carriers
JP5085871A JPH06233681A (ja) 1992-04-13 1993-04-13 Ｒｎａ担体によるインビトロ遺伝子選択方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI920892A IT1262937B (it) 1992-04-13 1992-04-13 Selezione genica in vitro mediante vettori ad rna

Publications (3)

Publication Number Publication Date
ITMI920892A0 ITMI920892A0 (it) 1992-04-13
ITMI920892A1 ITMI920892A1 (it) 1993-10-13
IT1262937B true IT1262937B (it) 1996-07-22

Family

ID=11362906

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI920892A IT1262937B (it) 1992-04-13 1992-04-13 Selezione genica in vitro mediante vettori ad rna

Country Status (3)

Country Link
EP (1) EP0566198A2 (it)
JP (1) JPH06233681A (it)
IT (1) IT1262937B (it)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
EP0461155B1 (en) * 1989-03-03 1994-07-27 Genentech, Inc. Improved methods for in vitro dna amplification and genomic cloning and mapping
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences

Also Published As

Publication number Publication date
ITMI920892A0 (it) 1992-04-13
EP0566198A2 (en) 1993-10-20
EP0566198A3 (it) 1994-08-03
ITMI920892A1 (it) 1993-10-13
JPH06233681A (ja) 1994-08-23

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Legal Events

Date Code Title Description
0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19971028