IT1248999B - Labelling of double helix DNA by transamination - Google Patents

Labelling of double helix DNA by transamination

Info

Publication number
IT1248999B
IT1248999B IT02076790A IT2076790A IT1248999B IT 1248999 B IT1248999 B IT 1248999B IT 02076790 A IT02076790 A IT 02076790A IT 2076790 A IT2076790 A IT 2076790A IT 1248999 B IT1248999 B IT 1248999B
Authority
IT
Italy
Prior art keywords
dna
double helix
transamination
labelling
helix dna
Prior art date
Application number
IT02076790A
Other languages
Italian (it)
Other versions
IT9020767A1 (en
IT9020767A0 (en
Inventor
Franco Armando Bignone
Original Assignee
Ist Naz Ric Sul Cancro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ist Naz Ric Sul Cancro filed Critical Ist Naz Ric Sul Cancro
Priority to IT02076790A priority Critical patent/IT1248999B/en
Publication of IT9020767A0 publication Critical patent/IT9020767A0/it
Publication of IT9020767A1 publication Critical patent/IT9020767A1/en
Application granted granted Critical
Publication of IT1248999B publication Critical patent/IT1248999B/en

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  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

A method for the preparation of double helix DNA molecules based on the creation of molecules containing N4-aminoethyl-cytosine (4-AEC) is described. After this initial pass the 4-AEC residues can be conjugated with the ester of hydrosuccinimide-biotin, giving rise to biotinylated DNA. The labelled DNA so obtained can be useful as a molecular weight standard, or as a control in Southern Blots. It may also be useful in the investigation of interactions between proteins and DNA for their purification through chromatography procedures.
IT02076790A 1990-06-26 1990-06-26 Labelling of double helix DNA by transamination IT1248999B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
IT02076790A IT1248999B (en) 1990-06-26 1990-06-26 Labelling of double helix DNA by transamination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT02076790A IT1248999B (en) 1990-06-26 1990-06-26 Labelling of double helix DNA by transamination

Publications (3)

Publication Number Publication Date
IT9020767A0 IT9020767A0 (en) 1990-06-26
IT9020767A1 IT9020767A1 (en) 1991-12-26
IT1248999B true IT1248999B (en) 1995-02-11

Family

ID=11171774

Family Applications (1)

Application Number Title Priority Date Filing Date
IT02076790A IT1248999B (en) 1990-06-26 1990-06-26 Labelling of double helix DNA by transamination

Country Status (1)

Country Link
IT (1) IT1248999B (en)

Also Published As

Publication number Publication date
IT9020767A1 (en) 1991-12-26
IT9020767A0 (en) 1990-06-26

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Legal Events

Date Code Title Description
0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19970626