IT1018621B - DEVICE FOR THE QUANTITATIVE DETERMINATION OF MICROBIAL PATHOGENS - Google Patents

DEVICE FOR THE QUANTITATIVE DETERMINATION OF MICROBIAL PATHOGENS

Info

Publication number
IT1018621B
IT1018621B IT48234/74A IT4823474A IT1018621B IT 1018621 B IT1018621 B IT 1018621B IT 48234/74 A IT48234/74 A IT 48234/74A IT 4823474 A IT4823474 A IT 4823474A IT 1018621 B IT1018621 B IT 1018621B
Authority
IT
Italy
Prior art keywords
pathogens
sample
filter medium
concentrated
liquid filter
Prior art date
Application number
IT48234/74A
Other languages
Italian (it)
Original Assignee
Wadley Res Inst & Blood Bank
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wadley Res Inst & Blood Bank filed Critical Wadley Res Inst & Blood Bank
Application granted granted Critical
Publication of IT1018621B publication Critical patent/IT1018621B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/852—Klebsiella
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/873—Proteus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/874—Pseudomonas
    • Y10S435/875—Pseudomonas aeruginosa
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/882—Staphylococcus
    • Y10S435/883—Staphylococcus aureus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/885—Streptococcus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y10S435/922—Candida albicans

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method and apparatus are disclosed which provide for the rapid quantitative detection of microbial pathogens in a sample fluid such as blood. Initially the pathogens are concentrated by depositing the sample fluid on a liquid filter medium such as a concentrated aqueous solution of sucrose or a microporous aqueous solution of a crosslinked polymer having a greater density than the sample, and microporous openings throughout its solubilized network (which range in size between about 1 micron and about 7 microns) and then the material is subjected to centrifugation to cause the pathogens in the sample to selectively pass into the liquid filter medium. This step not only concentrates the pathogens but also separates them from the other components of the sample such as antimicrobial constituents of blood and medicants present in blood samples, such as antibiotics. The liquid filter medium containing the concentrated pathogens is then added to nutrient media for culturing and quantitative counting.
IT48234/74A 1973-02-12 1974-02-08 DEVICE FOR THE QUANTITATIVE DETERMINATION OF MICROBIAL PATHOGENS IT1018621B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33169373A 1973-02-12 1973-02-12
US428135*[A US3928139A (en) 1973-02-12 1974-01-09 Detection of microbial pathogens

Publications (1)

Publication Number Publication Date
IT1018621B true IT1018621B (en) 1977-10-20

Family

ID=26987871

Family Applications (1)

Application Number Title Priority Date Filing Date
IT48234/74A IT1018621B (en) 1973-02-12 1974-02-08 DEVICE FOR THE QUANTITATIVE DETERMINATION OF MICROBIAL PATHOGENS

Country Status (7)

Country Link
US (1) US3928139A (en)
JP (1) JPS5642280B2 (en)
CA (1) CA1021238A (en)
DE (1) DE2406362A1 (en)
FR (1) FR2217419B1 (en)
GB (1) GB1458147A (en)
IT (1) IT1018621B (en)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
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US3875012A (en) * 1974-01-30 1975-04-01 Wadley Res Inst & Blood Bank Apparatus and method for the detection of microbial pathogens
US3932222A (en) * 1974-12-20 1976-01-13 J. K. & Susie L. Wadley Research Institute And Blood Bank For isolating pathogenic microorganisms
GB1566098A (en) * 1975-11-14 1980-04-30 Nat Res Dev Separation of solid and liquid components of mixtures
US4038150A (en) * 1976-03-24 1977-07-26 J. K. And Susie L. Wadley Research Institute And Blood Bank Sample mixing and centrifugation apparatus
US4131512A (en) * 1976-11-05 1978-12-26 J. K. And Susie L. Wadley Research Institute And Blood Bank Method for detecting microbial pathogens employing a cushioning agent
US4206282A (en) * 1977-08-29 1980-06-03 Pfizer Inc. Hypertonic culture media
US4164449A (en) * 1977-11-03 1979-08-14 J. K. And Susie L. Wadley Research Institute And Blood Bank Surface separation technique for the detection of microbial pathogens
SE452336B (en) * 1978-06-16 1987-11-23 Boehringer Mannheim Gmbh PROCEDURE FOR DISPOSAL OF DISEASE-BREATHING MICROORGANISMS WHICH ADSORBATED EXTRACORPORAL ON A SELECTIVE BINDING ADSORBENT
DK366778A (en) * 1978-08-18 1980-02-19 Foss Electric As METHOD OF COUNTING BACTERIES
US4212948A (en) * 1978-10-18 1980-07-15 J. K. And Susie L. Wadley Research Institute And Blood Bank Apparatus for detecting microbial pathogens employing a cushioning agent
US4218534A (en) * 1979-02-21 1980-08-19 Dairyland Food Laboratories, Inc. Phage detection
US4634417A (en) * 1982-12-06 1987-01-06 Georgetown University Process for treatment of tumors and apparatus therefor
US4610961A (en) * 1982-12-27 1986-09-09 Eastman Kodak Company Inhibition of reduction activities of leukocytes
US4503149A (en) * 1983-06-06 1985-03-05 Conoco Inc. Method of microbial assay
USRE34405E (en) * 1983-08-01 1993-10-12 Abbott Laboratories Determination of analytes in particle-containing medium
US4581331A (en) * 1983-09-29 1986-04-08 E. I. Du Pont De Nemours And Company Method for the rapid detection of virus and viral antigens
US5171669A (en) * 1987-05-04 1992-12-15 Eastman Kodak Company Cobalt(III) reagents in combination with water soluble polymers
US4912035A (en) * 1987-06-11 1990-03-27 Eastman Kodak Company Method for minimizing interference by reductants when detecting cells in biological fluids
US5141855A (en) * 1986-07-28 1992-08-25 Eastman Kodak Company Signal amplifying cobalt (III) redox reagents and methods for the determination of analytes in aqueous fluids
ATE147790T1 (en) * 1987-11-05 1997-02-15 James D Berg DIRECT METHOD FOR DETECTING VERY LOW LEVELS OF COLIFORM CONTAMINATION
US5518894A (en) * 1987-11-05 1996-05-21 Berg; James D. Rapid coliform detection system
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US7107824B1 (en) * 1998-01-14 2006-09-19 Johns Hopkins University Apparatus for the separation of cystic parasite forms from water
US6511819B2 (en) 1998-08-28 2003-01-28 Nye Colifast As Rapid coliform detection system
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US20030138906A1 (en) * 2001-11-05 2003-07-24 Ingun Tryland Fluorescence test for measuring heterotrophic bacteria in water
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CA2741036C (en) * 2008-10-31 2018-01-09 Biomerieux, Inc. Methods for separation, characterization, and/or identification of microorganisms using raman spectroscopy
WO2010062356A1 (en) * 2008-10-31 2010-06-03 Biomerieux, Inc. Methods for separation, characterization and/or identification of microorganisms using spectroscopy
US8647835B2 (en) 2008-10-31 2014-02-11 BIO MéRIEUX, INC. Methods for separation, characterization and/or identification of microorganisms using spectroscopy
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US3072538A (en) * 1960-12-30 1963-01-08 Grace W R & Co Isolation of bacteria

Also Published As

Publication number Publication date
DE2406362C2 (en) 1988-02-18
DE2406362A1 (en) 1974-08-15
JPS49110822A (en) 1974-10-22
GB1458147A (en) 1976-12-08
CA1021238A (en) 1977-11-22
FR2217419B1 (en) 1978-01-06
FR2217419A1 (en) 1974-09-06
AU6522974A (en) 1975-08-07
JPS5642280B2 (en) 1981-10-03
US3928139A (en) 1975-12-23

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