IN2014DN09920A - - Google Patents

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Publication number
IN2014DN09920A
IN2014DN09920A IN9920DEN2014A IN2014DN09920A IN 2014DN09920 A IN2014DN09920 A IN 2014DN09920A IN 9920DEN2014 A IN9920DEN2014 A IN 9920DEN2014A IN 2014DN09920 A IN2014DN09920 A IN 2014DN09920A
Authority
IN
India
Prior art keywords
fragments
plants
promoter
expression
nucleic acid
Prior art date
Application number
Inventor
Andrew Mark Cigan
Erica Unger-Wallace
Original Assignee
Pioneer Hi Bred Int
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Du Pont filed Critical Pioneer Hi Bred Int
Publication of IN2014DN09920A publication Critical patent/IN2014DN09920A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology

Abstract

The plant promoter of a CBSU- Anther_Subtraction library (CAS1) gene encoding a mannitol dehydrogenase , and fragments thereof , and their use in promoting the expression of one or more heterologous nucleic acid fragments in an inducible manner in plants are described. These promoter fragments are also useful in creating recombinant DNA constructs comprising nucleic acid sequences encoding a desired gene product operably linked to such promoter fragments which can be utilized to transform plants and bring the expression of the gene product under external chemical and/ or heat control in monocotyledonous and dicotyledonous plants.
IN9920DEN2014 2012-05-18 2013-05-16 IN2014DN09920A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261648758P 2012-05-18 2012-05-18
PCT/US2013/041267 WO2013173535A2 (en) 2012-05-18 2013-05-16 Inducible promoter sequences for regulated expression and methods of use

Publications (1)

Publication Number Publication Date
IN2014DN09920A true IN2014DN09920A (en) 2015-08-14

Family

ID=48577883

Family Applications (1)

Application Number Title Priority Date Filing Date
IN9920DEN2014 IN2014DN09920A (en) 2012-05-18 2013-05-16

Country Status (7)

Country Link
US (2) US20150101077A1 (en)
CN (1) CN104487577A (en)
BR (1) BR112014028615A2 (en)
CA (1) CA2873518A1 (en)
IN (1) IN2014DN09920A (en)
RU (1) RU2014151361A (en)
WO (1) WO2013173535A2 (en)

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AU2015288157A1 (en) 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
CN108064129A (en) 2014-09-12 2018-05-22 纳幕尔杜邦公司 The generation in the site-specific integration site of complex character locus and application method in corn and soybean
EP3262176A1 (en) * 2015-02-25 2018-01-03 Pioneer Hi-Bred International, Inc. Composition and methods for regulated expression of a guide rna/cas endonuclease complex
CN107567499A (en) 2015-03-27 2018-01-09 纳幕尔杜邦公司 Soybean U6 small nuclear RNAs gene promoter and its purposes in the constitutive expression of plant MicroRNA gene
KR20180008572A (en) 2015-05-15 2018-01-24 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 Rapid characterization of CAS endonuclease systems, PAM sequences and guide RNA elements
CN104894134A (en) * 2015-06-26 2015-09-09 南通大学 Diploid Urartu wheat phosphate mannose mutase gene promoter TuPMM-Al and application thereof
KR20180059535A (en) 2015-10-20 2018-06-04 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 Methods and compositions for marker-free genome modification
CN106916814B (en) * 2015-12-25 2021-05-25 丰益(上海)生物技术研发中心有限公司 Sodium citrate-inducible promoter
US20190161742A1 (en) 2016-03-11 2019-05-30 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
WO2017155717A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
WO2017155715A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
MX2018014993A (en) 2016-06-14 2019-09-06 Pioneer Hi Bred Int Use of cpf1 endonuclease for plant genome modifications.
US20190100745A1 (en) 2016-06-20 2019-04-04 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
BR112019022201A2 (en) 2017-04-24 2020-05-12 Dupont Nutrition Biosciences Aps METHODS FOR MODULATING THE ACTIVITY OF A CAS ENDONUCLEASE, TO INCREASE THE SPECIFICITY OF A CAS ENDONUCLEASE AND POLYNUCLEOTIDE COMPLEX GUIDE, TO INCREASE THE FREQUENCY OF HOMOLOGICAL RECOMBINATION AND FOR GENE ACTIVATION OR REPRESSION, CELL AND CELL CELL
JP2022514493A (en) 2018-12-14 2022-02-14 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド A novel CRISPR-CAS system for genome editing

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Also Published As

Publication number Publication date
US9493782B2 (en) 2016-11-15
US20150101077A1 (en) 2015-04-09
WO2013173535A3 (en) 2014-02-27
WO2013173535A2 (en) 2013-11-21
CA2873518A1 (en) 2013-11-21
US20130312137A1 (en) 2013-11-21
RU2014151361A (en) 2016-07-10
CN104487577A (en) 2015-04-01
BR112014028615A2 (en) 2017-06-27

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