IN2014DN08957A - - Google Patents
Info
- Publication number
- IN2014DN08957A IN2014DN08957A IN8957DEN2014A IN2014DN08957A IN 2014DN08957 A IN2014DN08957 A IN 2014DN08957A IN 8957DEN2014 A IN8957DEN2014 A IN 8957DEN2014A IN 2014DN08957 A IN2014DN08957 A IN 2014DN08957A
- Authority
- IN
- India
- Prior art keywords
- sample
- analyzing
- nucleic acid
- background signal
- acid stain
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Plural Heterocyclic Compounds (AREA)
Abstract
Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261620823P | 2012-04-05 | 2012-04-05 | |
PCT/US2013/035291 WO2013152203A1 (en) | 2012-04-05 | 2013-04-04 | Sample preparation for flow cytometry |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2014DN08957A true IN2014DN08957A (en) | 2015-05-22 |
Family
ID=49301050
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN8957DEN2014 IN2014DN08957A (en) | 2012-04-05 | 2013-04-04 |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP2834367A4 (en) |
JP (1) | JP6401146B2 (en) |
CN (1) | CN104379760B (en) |
AU (1) | AU2013243378B2 (en) |
CA (1) | CA2869472C (en) |
IN (1) | IN2014DN08957A (en) |
WO (1) | WO2013152203A1 (en) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
WO2008014485A2 (en) | 2006-07-28 | 2008-01-31 | California Institute Of Technology | Multiplex q-pcr arrays |
US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
US9645057B2 (en) | 2012-04-05 | 2017-05-09 | Becton, Dickiinson and Company | Method for improving analysis of microorganisms in complex matrices |
CN105189774A (en) * | 2013-03-13 | 2015-12-23 | 贝克顿·迪金森公司 | A method for improving analysis of microorganisms in complex matrices |
CN104677809B (en) * | 2013-11-29 | 2018-01-02 | 中国人民解放军第二军医大学 | Cryptococcus viability detection kit and detection method |
US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
CN107643238A (en) * | 2017-09-19 | 2018-01-30 | 中山大学附属第医院 | A kind of method for quantitatively detecting blood circulation particle concentration |
ES2967515T3 (en) * | 2018-08-01 | 2024-04-30 | Sartorius Bioanalytical Instr Inc | Methods, kits and staining compositions for flow cytometric evaluation of unassociated virus-sized particles using multiple fluorogenic dyes |
CN113924041A (en) | 2019-03-14 | 2022-01-11 | 因斯利克萨公司 | Method and system for fluorescence detection based on time gating |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3215428B2 (en) * | 1995-01-16 | 2001-10-09 | ソイニ,エルッキ | Biospecific multi-parameter assays |
US20030013849A1 (en) * | 1999-10-29 | 2003-01-16 | Ward William W. | Renilla reniformis green fluorescent protein |
RU2172512C1 (en) * | 2000-02-01 | 2001-08-20 | Закрытое акционерное общество Научно-производственное объединение "ФоМос" | Colored spectrum-zone silver halide photographic material |
US6323337B1 (en) * | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
CA2429526C (en) * | 2000-11-17 | 2011-03-15 | Biomoda, Inc. | Compositions and methods for detecting pre-cancerous conditions in cell and tissue samples using 5, 10, 15, 20-tetrakis (carboxyphenyl) porphine |
US7205100B2 (en) * | 2002-10-10 | 2007-04-17 | Advanced Analytical Technologies, Inc. | Methods for reducing background fluorescence |
US8481302B2 (en) * | 2008-11-03 | 2013-07-09 | General Electric Company | Total bacteria monitoring system |
GB201005939D0 (en) * | 2010-04-09 | 2010-05-26 | Biostatus Ltd | Method of analysing a cell or other biological material containing nucleic acid |
WO2011160887A1 (en) * | 2010-06-21 | 2011-12-29 | Siemens Healthcare Diagnostics Inc. | Soluble quencher to reduce background in qpcr assays |
-
2013
- 2013-04-04 IN IN8957DEN2014 patent/IN2014DN08957A/en unknown
- 2013-04-04 CN CN201380029083.0A patent/CN104379760B/en active Active
- 2013-04-04 AU AU2013243378A patent/AU2013243378B2/en active Active
- 2013-04-04 WO PCT/US2013/035291 patent/WO2013152203A1/en active Application Filing
- 2013-04-04 CA CA2869472A patent/CA2869472C/en active Active
- 2013-04-04 EP EP13773119.6A patent/EP2834367A4/en not_active Withdrawn
- 2013-04-04 JP JP2015504727A patent/JP6401146B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2013152203A1 (en) | 2013-10-10 |
EP2834367A4 (en) | 2016-03-23 |
CA2869472A1 (en) | 2013-10-10 |
CA2869472C (en) | 2017-07-11 |
EP2834367A1 (en) | 2015-02-11 |
CN104379760A (en) | 2015-02-25 |
JP2015514403A (en) | 2015-05-21 |
CN104379760B (en) | 2018-03-16 |
AU2013243378B2 (en) | 2019-04-11 |
AU2013243378A1 (en) | 2014-10-30 |
JP6401146B2 (en) | 2018-10-03 |
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